CN112807264A

CN112807264A - Hyaluronic acid-copper peptide composition and preparation method and application thereof

Info

Publication number: CN112807264A
Application number: CN202110267445.6A
Authority: CN
Inventors: 卢宇鸿; 陈锡武
Original assignee: Guangzhou Peiyan Medical Technology Co ltd
Current assignee: Guangzhou Peiyan Medical Technology Co ltd
Priority date: 2021-03-11
Filing date: 2021-03-11
Publication date: 2021-05-18
Anticipated expiration: 2041-03-11
Also published as: CN112807264B

Abstract

The invention discloses a hyaluronic acid blue copper peptide composition, a preparation method and application thereof, wherein the hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.02-0.2 part of disodium ethylene diamine tetraacetate, 0.05-0.2 part of phenoxyethanol, 0.05-0.5 part of sodium hyaluronate, 0.05-0.5 part of ceruloplasmin, 0.1-0.5 part of dipotassium glycyrrhizinate, 0.2-1 part of ethylhexyl glycerol, 0.5-2 parts of honeysuckle extract, 1-3 parts of tremella polysaccharide, 1-5 parts of 1, 2-pentanediol, 1-8 parts of beta-glucan and 35-50 parts of deionized water. The hyaluronic acid-copper peptide composition can effectively repair skin, delay skin aging, accelerate skin regeneration and eliminate wrinkles; the honeysuckle extract and the bluecopper peptide prepared by the invention have obvious synergistic effect on the aspect of repairing skin.

Description

Hyaluronic acid-copper peptide composition and preparation method and application thereof

Technical Field

The invention relates to the technical field of cosmetics, and in particular relates to a hyaluronic acid blue copper peptide composition and a preparation method and application thereof.

Background

The skin care product is applied on skin, and has effects of moistening and keeping moisture. Especially in dry autumn and winter, the skin care product can increase oil content and moisture of skin, prevent dryness and crack, and moisten skin with luster. However, the components and additives of skin care products vary widely, and the functionality varies with the added components.

The skin is the tissue which covers the largest part of the body surface, accounts for about 16 percent of the body weight, has luster, relatively moist and good toughness and elasticity, and plays a plurality of physiological roles of protecting, absorbing, secreting, excreting, sensing stimulation, regulating the body temperature, maintaining water and salt balance, participating in substance metabolism and the like in the physiological functions of the body.

With the rapid development of society, the living standard of people is continuously improved, the skin care is more and more popularized, and the skin care and repair are a vital step.

Disclosure of Invention

The invention provides a hyaluronic acid blue copper peptide composition, a preparation method and application thereof.

The invention adopts the following technical scheme for solving the technical problems:

the hyaluronic acid-copper peptide composition is prepared from the following raw materials in parts by weight: 0.02-0.2 part of disodium ethylene diamine tetraacetate, 0.05-0.2 part of phenoxyethanol, 0.05-0.5 part of sodium hyaluronate, 0.05-0.5 part of ceruloplasmin, 0.1-0.5 part of dipotassium glycyrrhizinate, 0.2-1 part of ethylhexyl glycerol, 0.5-2 parts of honeysuckle extract, 1-3 parts of tremella polysaccharide, 1-5 parts of 1, 2-pentanediol, 1-8 parts of beta-glucan and 35-50 parts of deionized water.

As a preferable scheme, the hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.02-0.1 part of disodium ethylene diamine tetraacetate, 0.08-0.2 part of phenoxyethanol, 0.05-0.4 part of sodium hyaluronate, 0.05-0.4 part of ceruloplasmin, 0.2-0.5 part of dipotassium glycyrrhizinate, 0.3-1 part of ethylhexyl glycerol, 0.5-1.2 parts of honeysuckle extract, 1-2 parts of tremella polysaccharide, 1-4 parts of 1, 2-pentanediol, 1-5 parts of beta-glucan and 38-50 parts of deionized water.

As a preferable scheme, the hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.05 part of disodium ethylene diamine tetraacetate, 0.1 part of phenoxyethanol, 0.1 part of sodium hyaluronate, 0.2 part of ceruloplasmin, 0.4 part of dipotassium glycyrrhizinate, 0.8 part of ethylhexyl glycerin, 1 part of honeysuckle extract, 1.4 parts of tremella polysaccharide, 2 parts of 1, 2-pentanediol, 3 parts of beta-glucan and 40.95 parts of deionized water.

As a preferred scheme, the preparation method of the honeysuckle extract comprises the following steps:

s1, cleaning honeysuckle, drying, and crushing to 60-100 meshes to obtain honeysuckle powder;

s2, mixing honeysuckle powder, CTAB, complex enzyme and deionized water according to the weight ratio of 10: (0.05-0.15): (0.1-0.4): (30-60), reacting for 4-10 hours at the temperature of 70-80 ℃, and performing ultrasonic treatment to obtain a mixture;

s3, adding the mixed solution into an absolute ethyl alcohol solution, standing for 4-10 hours, filtering, centrifuging, and taking supernatant;

s4, adjusting the pH value of the supernatant to 6.2-6.8, loading the supernatant onto macroporous adsorption resin at a rate of 0.5-1.5 BV/h, eluting at a rate of 1-2 BV/h with deionized water, eluting at a rate of 1-2 BV with 40-60 wt% ethanol solution, eluting at a rate of 1-3 BV with 40-60 wt% ethanol solution, collecting ethanol washing solution, and drying to obtain the honeysuckle extract.

As a preferable scheme, the complex enzyme is prepared from cellulase, glucoamylase and neutral protease according to the weight ratio of 1: (0.4-0.8): (0.2-0.5).

As a preferable scheme, the complex enzyme is prepared from cellulase, glucoamylase and neutral protease according to the weight ratio of 1: 0.6: 0.4.

As a preferable scheme, the ultrasonic treatment power is 400-800W, and the treatment time is 25-60 min.

Preferably, the weight ratio of the mixed solution to the absolute ethyl alcohol is 1: (3-6).

As a preferred scheme, the macroporous adsorption resin is AB-8 macroporous adsorption resin.

The invention also provides a preparation method of the hyaluronic acid blue copper peptide composition, which comprises the following steps:

s11, adding 1, 2-pentanediol, sodium hyaluronate, tremella polysaccharide and ethylene diamine tetraacetic acid into deionized water, heating to 80-85 ℃, stirring at 50-150 rpm for 30-60 min, and homogenizing at 1800-2500 rpm for 4-10 min;

s12, cooling to 60-65 ℃, adding beta-glucan, and homogenizing at the rotating speed of 800-1200 rpm for 1-4 min;

s13, cooling to 35-40 ℃, adding phenoxyethanol, ceruloplasmin, dipotassium glycyrrhizinate, ethylhexyl glycerol and honeysuckle extract, and uniformly stirring to obtain the hyaluronic acid ceruloplasmin composition.

The invention also provides application of the hyaluronic acid blue copper peptide composition in cosmetics.

1.2-pentanediol: 1.2-pentanediol is a high-purity odorless product specially used for cosmetics, has good moisture-retaining ability and self-preservation performance, and has a bacteriostatic function.

Sodium hyaluronate: the sodium hyaluronate molecule is a double-spiral structure and can lock and combine with water molecules, so that the sodium hyaluronate has extremely high water retention capacity. The water retention capacity can theoretically reach 100ml/g, and is therefore called Natural Moisturizing Factor (NMF). The macromolecular sodium hyaluronate can form a breathable film on the surface of the skin, helps the skin to lock water, prevents external bacteria, dust and ultraviolet rays from entering, and protects the skin from being damaged. The hyaluronic acid can rapidly penetrate into dermis, and has effects of eliminating wrinkle, improving metabolism, promoting skin cell nutrition absorption, enhancing skin elasticity, and delaying skin aging. Plays a certain role in the proliferation and differentiation of epidermal cells, the elimination of oxygen free radicals and the prevention and repair of skin injury.

Beta-glucan: the beta-glucan has high moisturizing performance, can stimulate macrophages, activate an immune system to generate cytokines, promote wound healing, eliminate skin inflammation, accelerate the after-sun repair of skin, improve the immune function of damaged cells, promote the synthesis of collagen and the proliferation of skin cells, has a powerful moisturizing effect, improves the moisture of skin cells and improves the elasticity of skin.

Dipotassium glycyrrhizinate: dipotassium glycyrrhizinate has excellent anti-inflammatory activity, eliminates inflammatory symptoms, prevents allergic reaction of cosmetics after long-term use, and achieves the effects of resisting inflammation and resisting allergy by inhibiting histamine release, inhibiting the activity of hyaluronidase and inhibiting the generation of leukotriene and prostaglandin.

Disodium EDTA: the disodium EDTA can regulate the pH value of cosmetics in skin care products to protect the skin from strong stimulation.

Blue copper peptide: the tripeptide-1 copper is also called as the red blood removing and allergy relieving, has the functions of treating allergy, repairing red blood streak, removing acne and diminishing inflammation, nourishing skin cells, increasing the resistance of skin cells and improving the skin resistance, and can also be used for repairing and calming the skin before and after the operation of a photoelectric project instrument, repairing and nursing after tattooing operation, and removing red and relaxing allergy. As a powerful tissue protection and anti-inflammatory agent, the composition is suitable for anti-inflammation and repair, promoting wound healing, relieving wrinkles, improving skin elasticity, and adding tripeptide-1 copper can effectively promote the production of collagen and elastin, increase antioxidant function, stimulate production of glucose polyamine, and help skin recover self-repairing ability.

Phenoxyethanol: phenoxyethanol is a colorless, transparent, oily liquid that can be mixed with water and polar organic solvents such as ethanol, propanol, propylene glycol, and the like. Phenoxyethanol does not irritate the skin, does not induce mutations in the organism, has antimicrobial activity against common bacteria, and is suitable for use in a wide pH range.

Ethyl hexyl glycerol: the ethyl hexyl glycerol is a multifunctional humectant, can be used as a natural preservative and a bactericide, and is used in cosmetics without an additive preservative system. The phenethyl alcohol is one of the components of natural spices and has certain bacteriostatic effect. Ethyl hexyl glycerol can enhance the preservative function of phenethyl alcohol, neither is in the list of preservatives prescribed by the regulations nor is a substance prohibited for cosmetics, and the composition has a certain preservative function and is often called as 'no preservative' or 'zero preservative'.

White fungus polysaccharide: tremella polysaccharides are commonly used in various skin care cosmetics and have the effects of repairing skin and enhancing moisture and moisture of surface skin. The tremella polysaccharide skin care product can improve the moisture of the horny layer, enable the skin to be softer and more elastic, has good lubricating feeling, can form a layer of protective film on the surface layer of the skin, can promote the regrowth of cells and accelerate the regeneration of the skin, thereby eliminating wrinkles and repairing the skin damaged by ultraviolet irradiation.

The invention has the beneficial effects that: the hyaluronic acid-copper peptide composition disclosed by the invention is scientific and reasonable in formula and good in compatibility, and can effectively repair skin, delay skin aging, accelerate skin regeneration and eliminate wrinkles; the honeysuckle extract and the bluecopper peptide prepared by the invention have obvious synergistic effect on the aspect of repairing skin.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.05 part of disodium ethylene diamine tetraacetate, 0.1 part of phenoxyethanol, 0.1 part of sodium hyaluronate, 0.2 part of ceruloplasmin, 0.4 part of dipotassium glycyrrhizinate, 0.8 part of ethylhexyl glycerin, 1 part of honeysuckle extract, 1.4 parts of tremella polysaccharide, 2 parts of 1, 2-pentanediol, 3 parts of beta-glucan and 40.95 parts of deionized water.

The preparation method of the honeysuckle extract comprises the following steps:

s1, cleaning honeysuckle, drying, and crushing to 90 meshes to obtain honeysuckle powder;

s2, mixing honeysuckle powder, CTAB, complex enzyme and deionized water according to the weight ratio of 10: 0.1: 0.3: 40, reacting for 6 hours at 75 ℃, and carrying out ultrasonic treatment for 35min at 500W to obtain a mixture; the compound enzyme is prepared from cellulase, glucoamylase and neutral protease according to the weight ratio of 1: 0.6: 0.4;

s3, adding the mixed solution into an absolute ethyl alcohol solution, standing for 8 hours, filtering, centrifuging, and taking supernatant; the weight ratio of the mixed solution to the absolute ethyl alcohol is 1: 4;

s4, adjusting the pH value of the supernatant to 6.6, loading the supernatant on macroporous adsorption resin at the rate of 1BV/h, washing the supernatant with deionized water at the rate of 1BV/h and washing the supernatant with 50 wt% of ethanol solution at the rate of 2BV, collecting ethanol washing liquid, and drying to obtain the honeysuckle extract.

The macroporous adsorption resin is AB-8 macroporous adsorption resin.

The preparation method of the hyaluronic acid blue copper peptide composition comprises the following steps:

s11, adding 1, 2-pentanediol, sodium hyaluronate, tremella polysaccharide and ethylene diamine tetraacetic acid into deionized water, heating to 82 ℃, stirring at 100rpm for 40min, and homogenizing at 2000rpm for 8 min;

s12, cooling to 63 ℃, adding beta-glucan, and homogenizing for 2min at the rotating speed of 1000 rpm;

s13, cooling to 38 ℃, adding phenoxyethanol, ceruloplasmin, dipotassium glycyrrhizinate, ethylhexyl glycerol and honeysuckle extract, and uniformly stirring to obtain the hyaluronic acid ceruloplasmin composition.

Example 2

The hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.02 part of disodium ethylene diamine tetraacetate, 0.05 part of phenoxyethanol, 0.05 part of sodium hyaluronate, 0.05 part of ceruloplasmin, 0.1 part of dipotassium glycyrrhizinate, 0.2 part of ethylhexyl glycerol, 0.5 part of honeysuckle extract, 1 part of tremella polysaccharide, 1 part of 1, 2-pentanediol, 1 part of beta-glucan and 35 parts of deionized water.

The macroporous adsorption resin is AB-8 macroporous adsorption resin.

Example 3

The hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.06 part of disodium ethylene diamine tetraacetate, 0.2 part of phenoxyethanol, 0.15 part of sodium hyaluronate, 0.1 part of ceruloplasmin, 0.4 part of dipotassium glycyrrhizinate, 1 part of ethylhexyl glycerin, 0.8 part of honeysuckle extract, 3 parts of tremella polysaccharide, 3 parts of 1, 2-pentanediol, 4 parts of beta-glucan and 50 parts of deionized water.

The macroporous adsorption resin is AB-8 macroporous adsorption resin.

Example 4

The hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.02 part of disodium ethylene diamine tetraacetate, 0.08 part of phenoxyethanol, 0.05 part of sodium hyaluronate, 0.05 part of ceruloplasmin, 0.2 part of dipotassium glycyrrhizinate, 0.3 part of ethylhexyl glycerol, 0.5 part of honeysuckle extract, 1 part of tremella polysaccharide, 1 part of 1, 2-pentanediol, 1 part of beta-glucan and 38 parts of deionized water.

The macroporous adsorption resin is AB-8 macroporous adsorption resin.

Comparative example 1

Comparative example 1 is different from example 1 in that comparative example 1 replaces the honeysuckle extract with the same amount of bluecopper peptide, and the rest is the same.

The hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.05 part of disodium ethylene diamine tetraacetate, 0.1 part of phenoxyethanol, 0.1 part of sodium hyaluronate, 1.2 parts of ceruloplasmin, 0.4 part of dipotassium glycyrrhizinate, 0.8 part of ethylhexyl glycerol, 1.4 parts of tremella polysaccharide, 2 parts of 1, 2-pentanediol, 3 parts of beta-glucan and 40.95 parts of deionized water.

Comparative example 2

Comparative example 2 is different from example 1 in that comparative example 2 replaces the copper peptide with the same amount of honeysuckle extract, and the others are the same.

The hyaluronic acid blue copper peptide composition is prepared from the following raw materials in parts by weight: 0.05 part of disodium ethylene diamine tetraacetate, 0.1 part of phenoxyethanol, 0.1 part of sodium hyaluronate, 0.4 part of dipotassium glycyrrhizinate, 0.8 part of ethylhexyl glycerol, 1.2 parts of honeysuckle extract, 1.4 parts of tremella polysaccharide, 2 parts of 1, 2-pentanediol, 3 parts of beta-glucan and 40.95 parts of deionized water.

Comparative example 3

Comparative example 3 is different from example 1 in that the honeysuckle extract of comparative example 3 is prepared by a method different from that of example 1, and the rest is the same.

s3, adding the mixed solution into an absolute ethyl alcohol solution, standing for 8 hours, filtering, centrifuging, taking supernatant, and drying to obtain a honeysuckle extract; the weight ratio of the mixed solution to the absolute ethyl alcohol is 1: 4.

comparative example 4

Comparative example 4 is different from example 1 in that the preparation method of the honeysuckle extract described in comparative example 4 is different from example 1, and the rest is the same.

s2, adding honeysuckle powder into deionized water, leaching for 100min at 75 ℃, filtering and drying to obtain a honeysuckle extract, wherein the weight ratio of the honeysuckle powder to the deionized water is 1: 9.

comparative example 5

s2, adding honeysuckle powder into 60wt% ethanol solution, extracting for 90min at 75 ℃, filtering and drying to obtain honeysuckle extract, wherein the weight ratio of the honeysuckle powder to the ethanol solution is 1: 9.

to further demonstrate the effect of the present invention, the following test methods were provided:

1. the 27 healthy white big-ear rabbits are raised in a single cage, water and diet are freely taken, the room temperature of a raising room is kept at 28 ℃, and the light and shade period is 24 hours. After the white rabbits with big ears are adapted to the environment for one week, 0.4% phenobarbital sodium (15 mL/kg) is injected into the abdominal cavity to anaesthetize the white rabbits, and the backs of the white rabbits are unhaired by a shaver with the area of 5cm multiplied by 5 cm. Then, the water in the kettle is boiled by an induction cooker and kept in a continuous boiling state, a kettle opening is connected to the bottom opening of a 50mL bottoming centrifuge tube through a heat insulation guide tube, the opening (the area is 6 cm) of the centrifuge tube is tightly pressed and contacted with the back (the wound surface at 2 parts of each big ear white rabbit, and the wound surfaces at 1 part of each left back and the right back) of the tested big ear white rabbit to remove hair, and the steam ironing lasts for 12 seconds each time, so that a deep II-degree scald model is established. The white rabbits with the scalded big ears are randomly divided into 9 groups, each group comprises 3 rabbits, the compositions prepared in the examples 1-4 and the comparative examples 1-5 are respectively smeared on the groups, and a single layer of sterile gauze covers the wound surface. The product is applied 3 times daily. Wound surface changes are observed and recorded every day after operation, then wound surface healing area is calculated through software, healing percentage is calculated after 7 days, and test results are shown in table 1.

2. 45 women with facial red blood streak of 20-30 years old age are selected as trial objects, the trial objects are divided into 9 groups at random, 5 persons in each group are respectively smeared on the parts with the facial red blood streak by the compositions prepared in the examples 1-4 and the comparative examples 1-5. Twice daily for 14 d. The using effects of the ingredients are divided into 5 points: the score of 5 (0.1-5) is the highest score, each score of 0.1 is one grade, the total score is calculated, and the result is shown in table 1.

TABLE 1 test results

As can be seen from table 1, the composition according to the invention has a good healing effect.

As can be seen from the comparison of examples 1 to 4, the ratio of different raw materials can affect the repairing effect of the composition, wherein example 1 is the best ratio.

As can be seen from the comparison of example 1 with comparative examples 1 and 2, the honeysuckle extract and the copper-coated peptide prepared by the invention have good synergistic effect in the aspect of repairing effect.

Comparing example 1 with comparative example 3, the invention adopts macroporous absorption resin treatment to improve the repairing effect.

Compared with comparative examples 4 and 5, the honeysuckle extract prepared by the preparation method disclosed by the invention has better repairing effect compared with the honeysuckle extract obtained by water extraction and alcohol extraction.

In light of the foregoing description of preferred embodiments according to the invention, it is clear that many changes and modifications can be made by the person skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.

Claims

1. The hyaluronic acid-copper peptide composition is characterized by being prepared from the following raw materials in parts by weight: 0.02-0.2 part of disodium ethylene diamine tetraacetate, 0.05-0.2 part of phenoxyethanol, 0.05-0.5 part of sodium hyaluronate, 0.05-0.5 part of ceruloplasmin, 0.1-0.5 part of dipotassium glycyrrhizinate, 0.2-1 part of ethylhexyl glycerol, 0.5-2 parts of honeysuckle extract, 1-3 parts of tremella polysaccharide, 1-5 parts of 1, 2-pentanediol, 1-8 parts of beta-glucan and 35-50 parts of deionized water.

2. The hyaluronic acid blue copper peptide composition according to claim 1, which is prepared from the following raw materials in parts by weight: 0.02-0.1 part of disodium ethylene diamine tetraacetate, 0.08-0.2 part of phenoxyethanol, 0.05-0.4 part of sodium hyaluronate, 0.05-0.4 part of ceruloplasmin, 0.2-0.5 part of dipotassium glycyrrhizinate, 0.3-1 part of ethylhexyl glycerol, 0.5-1.2 parts of honeysuckle extract, 1-2 parts of tremella polysaccharide, 1-4 parts of 1, 2-pentanediol, 1-5 parts of beta-glucan and 38-50 parts of deionized water.

3. The hyaluronic acid blue copper peptide composition according to claim 1, which is prepared from the following raw materials in parts by weight: 0.05 part of disodium ethylene diamine tetraacetate, 0.1 part of phenoxyethanol, 0.1 part of sodium hyaluronate, 0.2 part of ceruloplasmin, 0.4 part of dipotassium glycyrrhizinate, 0.8 part of ethylhexyl glycerin, 1 part of honeysuckle extract, 1.4 parts of tremella polysaccharide, 2 parts of 1, 2-pentanediol, 3 parts of beta-glucan and 40.95 parts of deionized water.

4. The hyaluronic acid blue copper peptide composition according to claim 1, wherein the preparation method of the honeysuckle extract comprises:

s2, mixing honeysuckle powder, CTAB, complex enzyme and deionized water according to the weight ratio of 10: (0.05-0.15): (0.1-0.4): (30-60), reacting for 4-10 h at 70-80 ℃, and carrying out ultrasonic treatment at 400-800W for 25-60 min to obtain a mixture;

5. The hyaluronic acid blue copper peptide composition according to claim 4, wherein the complex enzyme is prepared from cellulase, glucoamylase and neutral protease according to the weight ratio of 1: (0.4-0.8): (0.2-0.5).

6. The hyaluronic acid blue copper peptide composition according to claim 4, wherein the complex enzyme is prepared from cellulase, glucoamylase and neutral protease according to the weight ratio of 1: 0.6: 0.4.

7. The hyaluronic acid blue copper peptide composition of claim 4, wherein the weight ratio of the mixed solution to the absolute ethyl alcohol is 1: (3-6).

8. The hyaluronic acid blue copper peptide composition according to claim 4, wherein said macroporous adsorbent resin is AB-8 macroporous adsorbent resin.

9. The method for preparing hyaluronic acid blue copper peptide composition according to any of claims 1-8, wherein the method comprises the following steps:

10. Use of the hyaluronic acid blue copper peptide composition prepared according to claim 9 in cosmetics.