CN102869782B - 细菌产生软骨素的组合物和方法 - Google Patents

细菌产生软骨素的组合物和方法 Download PDF

Info

Publication number
CN102869782B
CN102869782B CN201180022154.5A CN201180022154A CN102869782B CN 102869782 B CN102869782 B CN 102869782B CN 201180022154 A CN201180022154 A CN 201180022154A CN 102869782 B CN102869782 B CN 102869782B
Authority
CN
China
Prior art keywords
gene
chrondroitin
cell
seqidno
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201180022154.5A
Other languages
English (en)
Chinese (zh)
Other versions
CN102869782A (zh
Inventor
D.H.多尔蒂
C.A.韦弗
宫本健太郎
南泽俊和
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Publication of CN102869782A publication Critical patent/CN102869782A/zh
Application granted granted Critical
Publication of CN102869782B publication Critical patent/CN102869782B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C07K16/1228—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K16/1232—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia from Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dermatology (AREA)
  • Materials Engineering (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
CN201180022154.5A 2010-03-01 2011-03-01 细菌产生软骨素的组合物和方法 Active CN102869782B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30940710P 2010-03-01 2010-03-01
US61/309,407 2010-03-01
PCT/US2011/026748 WO2011109438A1 (en) 2010-03-01 2011-03-01 Compositions and methods for bacterial production of chondroitin

Publications (2)

Publication Number Publication Date
CN102869782A CN102869782A (zh) 2013-01-09
CN102869782B true CN102869782B (zh) 2015-11-25

Family

ID=44542545

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180022154.5A Active CN102869782B (zh) 2010-03-01 2011-03-01 细菌产生软骨素的组合物和方法

Country Status (8)

Country Link
US (2) US8697398B2 (ko)
EP (1) EP2542686B1 (ko)
JP (1) JP5875531B2 (ko)
KR (1) KR101848102B1 (ko)
CN (1) CN102869782B (ko)
BR (1) BR112012021995A8 (ko)
ES (1) ES2661593T3 (ko)
WO (1) WO2011109438A1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104974972A (zh) * 2010-07-09 2015-10-14 灵知股份公司 软骨素的生物技术制备

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012021995A8 (pt) 2010-03-01 2018-01-02 Dsm Ip Assets Bv Composição,célula hospedeira bacteriana não patogênica, método para a produção de um sulfato de condroitina,condroitina, composição e anticorpo ou fragmento de anticorpo
JP5588578B2 (ja) * 2012-05-17 2014-09-10 東洋紡株式会社 担子菌酵母変異体
ITMI20120896A1 (it) 2012-05-23 2013-11-24 Bongulielmi Reto Condroitina per uso in medicina
ITMI20121316A1 (it) 2012-07-27 2014-01-28 Altergon Sa Complessi di condroitina ad assorbimento transcutaneo
EP2919806A4 (en) * 2012-11-16 2016-06-22 Jackson H M Found Military Med PEPTIDES GONORRHEA MOTHER AND VACCINES
JP2016503650A (ja) * 2012-12-28 2016-02-08 アンホエ ファホン バイオエンジニアリング カンパニー リミテッド Ｄｌ−アラニンを生産する遺伝子操作細菌、及び前記遺伝子操作細菌を用いることによってｄｌ−アラニンを生産する方法
WO2015046602A1 (ja) 2013-09-30 2015-04-02 生化学工業株式会社 タンパク質の血中滞留性の増強方法
FR3016637B1 (fr) * 2014-01-23 2020-11-27 Centre Nat Rech Scient Procede de production in vivo de glycosaminoglycane
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
EP3050973A1 (en) * 2015-01-30 2016-08-03 Jennewein Biotechnologie GmbH Fermentation process for producing monosaccharides in free form from nucleotide-activated sugars
EP3279220A4 (en) 2015-03-31 2018-12-19 Seikagaku Corporation Method for sulfating glycosaminoglycan
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
IT202000013633A1 (it) * 2020-06-08 2021-12-08 Vivatis Pharma Gmbh Processo di estrazione di una condroitina solfato da un fungo, una condroitina solfato di origine vegetale e suo uso
EP4067487A1 (en) 2021-04-01 2022-10-05 Givaudan SA Chondroitin-producing recombinant cell

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101120013A (zh) * 2004-12-16 2008-02-06 食品和营养托浦研究所基金会 通过在非病原性、非侵入性革兰氏阳性菌中异源表达和分泌复合多糖而有效产生病原性革兰氏阳性菌荚膜多糖的新方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104601A1 (en) 1999-04-01 2003-06-05 Deangelis Paul L. Chondroitin synthase gene and methods of making and using same
US20060105431A1 (en) 1998-11-11 2006-05-18 Deangelis Paul L Polymer grafting by polysaccharide synthases using artificial sugar acceptors
DE69940353D1 (en) 1998-11-11 2009-03-12 Univ Oklahoma Polymerpfropfung mittels polysaccharide-synthase
US6235510B1 (en) 1999-08-18 2001-05-22 Smithkline Beecham Corporation ppGaNTase-T6
EP1233072B1 (en) 1999-11-25 2006-08-02 Yamasa Corporation Novel use of uridine diphosphate glucose 4-epimerase
DE10019881A1 (de) * 2000-04-20 2001-11-15 Gerhard Miksch Verfahren zur Überexpression und extrazellulären Produktion bakterieller Phytasen in Escherichia coli
EP1470235A4 (en) 2001-07-13 2008-05-21 Univ Oklahoma GLYCOSAMINOGLYCAN POLYMERS AND METHODS OF PREPARING AND USING THE SAME
JP4702819B2 (ja) 2001-08-01 2011-06-15 生化学工業株式会社 コンドロイチン合成酵素
JP4101548B2 (ja) 2001-08-10 2008-06-18 生化学工業株式会社 コンドロイチン合成酵素及びそれをコードするｄｎａ
US7232676B2 (en) 2002-05-31 2007-06-19 Seikagaku Kogyo Kabushiki Kaisha Chondroitin synthase and nucleic acid encoding the enzyme
US7273729B2 (en) 2002-05-31 2007-09-25 Seikagaku Kogyo Kabushiki Kaisha Chondroitin synthase and DNA encoding the enzyme
WO2006033693A2 (en) 2004-06-30 2006-03-30 Deangelis Paul L Methods of selectively treating diseases with specific glycosaminoglycan polymers
ES2299284B1 (es) * 2004-11-04 2009-04-16 Universidad Pablo De Olavide Control de la expresion genica mediante el uso de un atenuador de la transcripcion.
BRPI0611112A2 (pt) * 2005-06-06 2010-08-10 Dow Global Technologies Inc sistemas promotores induzidos por manitol em células hospedeiras bacterianas
EP1964924B1 (en) 2005-12-15 2015-09-30 Seikagaku Corporation Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin
WO2008130373A2 (en) 2006-10-03 2008-10-30 The Board Of Regents Of The University Of Oklahoma Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same
CN101679955B (zh) 2007-04-24 2015-06-17 生化学工业株式会社 生产软骨素的细菌及生产软骨素的方法
BR112012021995A8 (pt) 2010-03-01 2018-01-02 Dsm Ip Assets Bv Composição,célula hospedeira bacteriana não patogênica, método para a produção de um sulfato de condroitina,condroitina, composição e anticorpo ou fragmento de anticorpo
WO2012004063A1 (en) 2010-07-09 2012-01-12 Gnosis S.P.A. Biotechnological production of chondroitin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101120013A (zh) * 2004-12-16 2008-02-06 食品和营养托浦研究所基金会 通过在非病原性、非侵入性革兰氏阳性菌中异源表达和分泌复合多糖而有效产生病原性革兰氏阳性菌荚膜多糖的新方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Molecular Cloning and Characterization of Chondroitin Polymerase from Escherichia coli Strain K4;Toshio Ninomiya et al.;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20020409;第277卷(第24期);第21573页表II *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104974972A (zh) * 2010-07-09 2015-10-14 灵知股份公司 软骨素的生物技术制备

Also Published As

Publication number Publication date
US9175293B2 (en) 2015-11-03
KR101848102B1 (ko) 2018-04-11
US8697398B2 (en) 2014-04-15
ES2661593T3 (es) 2018-04-02
US20110244520A1 (en) 2011-10-06
EP2542686A4 (en) 2014-03-26
JP2013520995A (ja) 2013-06-10
WO2011109438A1 (en) 2011-09-09
BR112012021995A2 (pt) 2015-10-06
EP2542686A1 (en) 2013-01-09
JP5875531B2 (ja) 2016-03-02
KR20130055571A (ko) 2013-05-28
EP2542686B1 (en) 2018-01-03
BR112012021995A8 (pt) 2018-01-02
US20140296505A1 (en) 2014-10-02
CN102869782A (zh) 2013-01-09

Similar Documents

Publication Publication Date Title
CN102869782B (zh) 细菌产生软骨素的组合物和方法
JP2020127403A (ja) フコシル化オリゴ糖の生産における使用に適したアルファ（１，２）フコシルトランスフェラーゼ
US20160237465A1 (en) Process for the production of hyaluronic acid in escherichia coli or bacillus subtilis
US11278610B2 (en) Glycosylation method
US9163270B2 (en) Process for the production of hyaluronic acid in Escherichia coli or Bacillus megaterium
CN107267579A (zh) 微生物发酵生产n‑乙酰‑d‑氨基葡萄糖和/或d‑氨基葡萄糖盐的方法
JP2023511523A (ja) Ｈｍｏの産生
CN104974973A (zh) 生产软骨素的细菌及生产软骨素的方法
CN106086102B (zh) 一种生产反式-4-羟基-l-脯氨酸的工程菌及其构建方法与应用
CN103937734B (zh) 一种生产透明质酸的基因工程菌及其应用
CN104822839A (zh) 用于产生单糖的方法
CN102191208A (zh) 高产多杀菌素的基因工程菌及其制备方法
CN112280795A (zh) 糖基转移酶基因的用途
CN109486734B (zh) 一种生产软骨素的基因工程菌及其构建方法和应用
DK181242B1 (en) GENETICALLY ENGINEERED CELLS COMPRISING A RECOMBINANT NUCLEIC ACID SEQUNCE ENCODING AN α-1,2-FUCOSYLTRANSFERASE CAPABLE OF PRODUCING LNFP-I, NUCLEIC ACID SEQUENCES ENCODING SAME AND METHODS FOR USE OF SAME
CN113637620B (zh) 一种克拉酸高产菌株构建方法和应用
CN112725372B (zh) 提高威兰胶表达的多酶复合体载体及高产威兰胶的重组菌
CN112359007B (zh) 用于产杆菌肽的外源引入edd基因地衣芽孢杆菌及应用
CN114806991A (zh) 一种提高岩藻糖基乳糖产量的工程大肠杆菌及生产方法
CN103849591B (zh) 一种泰乐菌素产生菌、遗传改造方法及其应用
CN117051043B (zh) 一种基于环状rna编码耐甲氧西林金黄色葡萄球菌内溶素及其应用
CN114806997A (zh) 一株纳他霉素胞外高效转运的褐黄孢链霉菌构建及应用
JP2023511525A (ja) ＨＭＯ生産における新しい主要促進剤スーパーファミリー（ＭＦＳ）タンパク質（Ｆｒｅｄ）
EA046260B1 (ru) Получение огм
WO2023083977A1 (en) Combined fermentation process for producing one or more human milk oligosaccharide(s) (hmo(s))

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Free format text: FORMER OWNER: SEIKAGAKU KOGYO CO. LTD.

Effective date: 20140819

Owner name: SEIKAGAKU KOGYO CO. LTD.

Free format text: FORMER OWNER: DSM IP PROPERTY COMPANY

Effective date: 20140819

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20140819

Address after: Tokyo, Japan, Japan

Applicant after: Seikagaku Kogyo Co., Ltd.

Address before: Holland Heerlen

Applicant before: DSM IP asset company

Applicant before: Seikagaku Kogyo Co., Ltd.

C14 Grant of patent or utility model
GR01 Patent grant