CN102845202B - Segmental-setting cultivation technique for Siraitia grosvenorii tissue culture seedlings - Google Patents
Segmental-setting cultivation technique for Siraitia grosvenorii tissue culture seedlings Download PDFInfo
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- CN102845202B CN102845202B CN201210330205.7A CN201210330205A CN102845202B CN 102845202 B CN102845202 B CN 102845202B CN 201210330205 A CN201210330205 A CN 201210330205A CN 102845202 B CN102845202 B CN 102845202B
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Abstract
The invention discloses a segmental-setting cultivation technique for Siraitia grosvenorii tissue culture seedlings. After each Siraitia grosvenorii bud grows to a shed and grows 6-15 sections, the buds are topped, and 2-6 lateral buds in the same direction are reserved as bearing shoots. The bearing shoots, which blossom, are used as first bearing segments. Each bearing segment is topped when growing 5-20 sections of flowers. 5-30 flowers are reserved on each bearing segment, and then the lateral buds are removed. The flowers and the lateral buds are removed from the terminal section of each bearing segment, and then the bearing segment is used as a second bearing segment. Four to six bearing segments are reserved in the same way, the terminal bearing segment is not subjected to topping and bud removal and is allowed to grow freely. At last, the bearing segments are subjected to segmental fruit setting. Each tissue culture seedling of Siraitia grosvenorii cultivated by the method can bear 150-250 fruits, the fruit production of single plants of Siraitia grosvenorii is increased greatly, the yield per unit area of Siraitia grosvenorii is increased, planting benefit per unit area is increased, and cultivate area is reduced.
Description
Technical field
The present invention relates to fruit tree planting technique field, particularly a kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique.
Background technology
Momordica grosvenori is a kind of perennial liana deciduous plant, and Dicotyledoneae, Cucurbitales, Curcurbitaceae, Momordica grosvenori belong to, and originates in the torrid zone, the Subtropical mountains that economize in Chinese Guangxi, Guangdong, Jiangxi etc., after the most famous with Guangxi forest products Momordica grosvenori.Momordica grosvenori is except fruit energy is medicinal, and grosvenor momordica leaf, Momordica grosvenori root also can be medicinal.Momordica grosvenori is the first batch of medicine-food two-purpose rare traditional Chinese medicine of announcing of the Ministry of Public Health, and sweet 300 times than sucrose of its contained Momordica-Glycosides, do not produce heat, are the famous and precious raw materials of beverage, confectionery industry, are the best substitute of sucrose.Normal drink " Luohan " fruit tea, can anti-various diseases.Modern medicine proves, Momordica grosvenori has significant curative effect to the disease such as bronchitis, hypertension, can also play the effect that prevents and treats coronary heart disease, vascular sclerosis, obesity.
Conventionally, adopt Momordica grosvenori traditional cultivation method, the fruit yield of individual plant Momordica grosvenori is about 60～80, adopt Momordica grosvenori standard culture technique, the fruit yield of individual plant Momordica grosvenori is about 80～100, and adopts high-yield culture technique, and the fruit yield of individual plant Momordica grosvenori is about 100～120.Existing market is large to the demand of Momordica grosvenori, and land resources is less, and the output of individual plant Momordica grosvenori still has much room for improvement.
Summary of the invention
For above-mentioned defect, the feature that the present invention takes full advantage of plant can term sorption nutrient, manufacture nutrient by stages, supply nutrient by stages, be used for cultivating Luohanguo With Plantlets of Tissue Culture, the fruit yield of every strain Momordica grosvenori can reach 150～250, increase substantially Luohanguo With Plantlets of Tissue Culture per plant quantity, improve Momordica grosvenori yield per unit area, reduced unit Momordica grosvenori production cost simultaneously, improved unit are planting benefit.
The present invention is achieved by the following technical programs:
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique comprises the following steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, planting site without the soil of pathogeny as Luohanguo With Plantlets of Tissue Culture.
(2) in 3～April, Luohanguo With Plantlets of Tissue Culture is colonizated in planting site, spacing in the rows is 1.5～3 meters, and the base manure of 3～6 jin is executed in every strain, then enriches the quick-acting composite fertilizers of 0.1～1 jin; Base manure is pig, ox or the people's of becoming thoroughly decomposed ight soil.Can make nursery stock maximize the vegetative period within year at field planting in 3～April Luohanguo With Plantlets of Tissue Culture.Momordica grosvenori seedling is higher to the demand of the fertilizer such as nitrogen, phosphorus, potassium growth early stage, enriches quick-acting composite fertilizers and can meet the demand of Momordica grosvenori seedling, and nursery stock is grown up healthy and sound quickly.
(3) at field scaffolding, in the time that Momordica grosvenori seedling grows to 15～25 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
(4) in the time that Momordica grosvenori nursery stock grows to 50～150 centimetres, carry out lower pinch of top of canopy for the first time, leave a young shoot and upwards grow, execute the flower forcing fertilizer of 0.1～1 jin to every strain Momordica grosvenori simultaneously.Now execute flower forcing fertilizer and can make that the secondary of nursery stock after upper canopy is climing normally to be spent.
(5) in the time that Momordica grosvenori nursery stock grows under canopy 0～40 centimetre, carry out lower pinch of top of canopy for the second time, leave canopy on two young shoots.Lower pinch of top of twice canopy, can promote seedling root prosperity, and blade face thickens, and seedling stem increases thick.
On (6) two young shoots, after canopy, it is fixed into straight line toward left and right rightabout, climing as one-level.
(7) every one-level is climing pinches top while growing to 6～15 joint, and every climing 2～6 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 4～12 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as first paragraph fruit bearing shoot, when every fruit bearing shoot has 5～20 joint flower, pinch top, every is stayed 5～30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as second segment fruit bearing shoot.
(9) when second segment fruit bearing shoot has 5～20 joint flower, pinch top, every is stayed 5～30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section of fruit bearing shoot.
(10) reserve 4 sections～6 sections fruit bearing shoot by step (8), step (9), final stage fruit bearing shoot is not pinched top, is not removed bud, lets alone growth.
(11) in the time of the setting phase of every section of fruit bearing shoot, every strain Momordica grosvenori is used 0.1～1 jin of quick-acting composite fertilizer.Momordica grosvenori fruiting period needs a large amount of liquid manure supplies, according to the setting phase segmentation fertilising of every section of fruit bearing shoot, can ensure the demand of Momordica grosvenori to fertilizer.
(12) when the setting phase, final stage fruit bearing shoot fruit number is not controlled, and every section of other section of fruit bearing shoot stays 5～20 of fruits.
Beneficial effect of the present invention:
The present invention adopts the method for segmentation setting, make on every section of fruit bearing shoot flowering time relatively concentrated, shorten that point on every section of fruit bearing shoot is taken time and the time of Fruit, concentrate the fruit on liquid manure and each section of fruit bearing shoot of nutrient supply, effectively extended the setting phase of Momordica grosvenori, improved the fruiting quantities of individual plant Momordica grosvenori, increase substantially Momordica grosvenori yield per unit area, reduce unit Momordica grosvenori production cost simultaneously, improve unit are planting benefit, reduce cultivated land occupancy area.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to the scope that embodiment represents.
Embodiment 1
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique comprises the following steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, planting site without the soil of pathogeny as Luohanguo With Plantlets of Tissue Culture.
(2) in March, Luohanguo With Plantlets of Tissue Culture is colonizated in planting site, spacing in the rows is 1.5 meters, and the base manure of 3 jin is executed in every strain, and base manure is the swine excrement becoming thoroughly decomposed, then enriches the quick-acting composite fertilizers of 0.1 jin.
(3) at field scaffolding, in the time that Momordica grosvenori seedling grows to 15 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
(4) in the time that Momordica grosvenori nursery stock grows to 50 centimetres, carry out lower pinch of top of canopy for the first time, leave a young shoot and upwards grow, execute the flower forcing fertilizer of 0.1 jin to every strain Momordica grosvenori simultaneously.
(5) in the time that Momordica grosvenori nursery stock grows to lower 0 centimetre of canopy, carry out lower pinch of top of canopy for the second time, leave canopy on two young shoots.
On (6) two young shoots, after canopy, it is fixed into straight line toward left and right rightabout, climing as one-level.
(7) every one-level is climing pinches top while growing to 6 joint, and every climing 2 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 4 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as first paragraph fruit bearing shoot, when every fruit bearing shoot has 5 joint flower, pinch top, every is stayed 5 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as second segment fruit bearing shoot.
(9) when second segment fruit bearing shoot has 5 joint flower, pinch top, every is stayed 5 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section of fruit bearing shoot.
(10) reserve 4 sections of fruit bearing shoot by step (8), step (9), final stage fruit bearing shoot is not pinched top, is not removed bud, lets alone growth.
(11) in the time of the setting phase of every section of fruit bearing shoot, every strain Momordica grosvenori is used 0.1 jin of quick-acting composite fertilizer.
(12) when the setting phase, final stage fruit bearing shoot fruit number is not controlled, and every section of other section of fruit bearing shoot stays 5 of fruits.
By this technology, when Momordica grosvenori results, the fruit yield of every strain Momordica grosvenori is about 150.
Embodiment 2
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique comprises the following steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, planting site without the soil of pathogeny as Luohanguo With Plantlets of Tissue Culture.
(2) in April, Luohanguo With Plantlets of Tissue Culture is colonizated in planting site, spacing in the rows is 3 meters, and the base manure of 6 jin is executed in every strain, and base manure is the ight soil of the ox of becoming thoroughly decomposed, then enriches the quick-acting composite fertilizers of 1 jin.
(3) at field scaffolding, in the time that Momordica grosvenori seedling grows to 25 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
(4) in the time that Momordica grosvenori nursery stock grows to 150 centimetres, carry out lower pinch of top of canopy for the first time, leave a young shoot and upwards grow, execute the flower forcing fertilizer of 1 jin to every strain Momordica grosvenori simultaneously.
(5) in the time that Momordica grosvenori nursery stock grows to lower 40 centimetres of canopy, carry out lower pinch of top of canopy for the second time, leave canopy on two young shoots.
On (6) two young shoots, after canopy, it is fixed into straight line toward left and right rightabout, climing as one-level.
(7) every one-level is climing pinches top while growing to 15 joint, and every climing 6 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 12 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as first paragraph fruit bearing shoot, when every fruit bearing shoot has 20 joint flower, pinch top, every is stayed 30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as second segment fruit bearing shoot.
(9) when second segment fruit bearing shoot has 20 joint flower, pinch top, every is stayed 30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section of fruit bearing shoot.
(10) reserve 6 sections of fruit bearing shoot by step (8), step (9), final stage fruit bearing shoot is not pinched top, is not removed bud, lets alone growth.
(11) in the time of the setting phase of every section of fruit bearing shoot, every strain Momordica grosvenori is used 1 jin of quick-acting composite fertilizer.
(12) when the setting phase, final stage fruit bearing shoot fruit number is not controlled, and every section of other section of fruit bearing shoot stays 20 of fruits.
By this technology, when Momordica grosvenori results, the fruit yield of every strain Momordica grosvenori is about 250.
Embodiment 3
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique comprises the following steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, planting site without the soil of pathogeny as Luohanguo With Plantlets of Tissue Culture.
(2) in March, Luohanguo With Plantlets of Tissue Culture is colonizated in planting site, spacing in the rows is 2 meters, and the base manure of 4 jin is executed in every strain, and base manure is the people's of becoming thoroughly decomposed ight soil, then enriches the quick-acting composite fertilizers of 0.5 jin.
(3) at field scaffolding, in the time that Momordica grosvenori seedling grows to 20 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
(4) in the time that Momordica grosvenori nursery stock grows to 100 centimetres, carry out lower pinch of top of canopy for the first time, leave a young shoot and upwards grow, execute the flower forcing fertilizer of 0.5 jin to every strain Momordica grosvenori simultaneously.
(5) in the time that Momordica grosvenori nursery stock grows to lower 20 centimetres of canopy, carry out lower pinch of top of canopy for the second time, leave canopy on two young shoots.
On (6) two young shoots, after canopy, it is fixed into straight line toward left and right rightabout, climing as one-level.
(7) every one-level is climing pinches top while growing to 10 joint, and every climing 4 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 8 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as first paragraph fruit bearing shoot, when every fruit bearing shoot has 10 joint flower, pinch top, every is stayed 20 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as second segment fruit bearing shoot.
(9) when second segment fruit bearing shoot has 10 joint flower, pinch top, every is stayed 20 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section of fruit bearing shoot.
(10) reserve 5 sections of fruit bearing shoot by step (8), step (9), final stage fruit bearing shoot is not pinched top, is not removed bud, lets alone growth.
(11) in the time of the setting phase of every section of fruit bearing shoot, every strain Momordica grosvenori is used 0.5 jin of quick-acting composite fertilizer.
(12) when the setting phase, final stage fruit bearing shoot fruit number is not controlled, and every section of other section of fruit bearing shoot stays 10 of fruits.
By this technology, when Momordica grosvenori results, the fruit yield of every strain Momordica grosvenori is about 200.
Claims (3)
1. a Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique, is characterized in that: this culture technique comprises the following steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, planting site without the soil of pathogeny as Luohanguo With Plantlets of Tissue Culture;
(2) Luohanguo With Plantlets of Tissue Culture is colonizated in planting site, spacing in the rows is 1.5～3 meters, and the base manure of 3～6 jin is executed in every strain, then enriches the quick-acting composite fertilizers of 0.1～1 jin;
(3) at field scaffolding, in the time that Momordica grosvenori seedling grows to 15～25 centimetres, at the fixing Momordica grosvenori seedling of field inserted link;
(4) in the time that Momordica grosvenori nursery stock grows to 50～150 centimetres, carry out lower pinch of top of canopy for the first time, leave a young shoot and upwards grow, execute the flower forcing fertilizer of 0.1～1 jin to every strain Momordica grosvenori simultaneously;
(5) in the time that Momordica grosvenori nursery stock grows under canopy 0～40 centimetre, carry out lower pinch of top of canopy for the second time, leave canopy on two young shoots;
On (6) two young shoots, after canopy, it is fixed into straight line toward left and right rightabout, climing as one-level;
(7) every one-level is climing pinches top while growing to 6～15 joint, and every climing 2～6 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 4～12 climing as a result of branches of secondary;
(8) after fruit bearing shoot is spent, as first paragraph fruit bearing shoot, when every fruit bearing shoot has 5～20 joint flower, pinch top, every is stayed 5～30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as second segment fruit bearing shoot;
(9) when second segment fruit bearing shoot has 5～20 joint flower, pinch top, every is stayed 5～30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section of fruit bearing shoot;
(10) reserve 4 sections～6 sections fruit bearing shoot by step (8), step (9), final stage fruit bearing shoot is not pinched top, is not removed bud, lets alone growth;
(11) in the time of the setting phase of every section of fruit bearing shoot, every strain Momordica grosvenori is used 0.1～1 jin of quick-acting composite fertilizer;
(12) when the setting phase, final stage fruit bearing shoot fruit number is not controlled, and every section of other section of fruit bearing shoot stays 5～20 of fruits.
2. Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique according to claim 1, is characterized in that: the described Luohanguo With Plantlets of Tissue Culture field planting of step (2) was carried out in 3～April.
3. Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique according to claim 2, is characterized in that: the described base manure of step (2) is pig, ox or the people's of becoming thoroughly decomposed ight soil.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104641858A (en) * | 2014-06-10 | 2015-05-27 | 蒙柳宁 | Fructus momordicae tissue culture seedling transplanting method |
| CN104322268A (en) * | 2014-11-28 | 2015-02-04 | 陶进科 | Momordica grosvenori mosaic virus avoiding method |
| CN104904544A (en) * | 2015-06-02 | 2015-09-16 | 广西大学 | Segmented fruit-setting cultivation technique for passion fruit tissue culture seedlings |
| CN105103845A (en) * | 2015-07-27 | 2015-12-02 | 广西鹿寨方田贸易有限公司 | Momordica grosvenori cultivation method |
| CN106258736A (en) * | 2016-08-12 | 2017-01-04 | 柳州市勤瑞农副产品有限公司 | A kind of cultural method of Passifolra edulis tissue cultured seedling segmentation setting |
| CN109156285A (en) * | 2018-08-24 | 2019-01-08 | 永福县农业局 | A kind of method of Siraitia grosvenorii off-season cultivation |
| CN111373996A (en) * | 2020-04-20 | 2020-07-07 | 广西壮族自治区中国科学院广西植物研究所 | High-yield cultivation method for momordica grosvenori |
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