CN102845202A - Segmental-setting cultivation technique for Siraitia grosvenorii tissue culture seedlings - Google Patents

Segmental-setting cultivation technique for Siraitia grosvenorii tissue culture seedlings Download PDF

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CN102845202A
CN102845202A CN2012103302057A CN201210330205A CN102845202A CN 102845202 A CN102845202 A CN 102845202A CN 2012103302057 A CN2012103302057 A CN 2012103302057A CN 201210330205 A CN201210330205 A CN 201210330205A CN 102845202 A CN102845202 A CN 102845202A
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fruit bearing
bearing shoot
momordica grosvenori
tissue culture
fruit
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CN102845202B (en
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廖苠焰
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Deng Xueming
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GUILIN XINGAN YUANSHENGYUAN BIOTECHNOLOGY CO Ltd
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Abstract

The invention discloses a segmental-setting cultivation technique for Siraitia grosvenorii tissue culture seedlings. After each Siraitia grosvenorii bud grows to a shed and grows 6-15 sections, the buds are topped, and 2-6 lateral buds in the same direction are reserved as bearing shoots. The bearing shoots, which blossom, are used as first bearing segments. Each bearing segment is topped when growing 5-20 sections of flowers. 5-30 flowers are reserved on each bearing segment, and then the lateral buds are removed. The flowers and the lateral buds are removed from the terminal section of each bearing segment, and then the bearing segment is used as a second bearing segment. Four to six bearing segments are reserved in the same way, the terminal bearing segment is not subjected to topping and bud removal and is allowed to grow freely. At last, the bearing segments are subjected to segmental fruit setting. Each tissue culture seedling of Siraitia grosvenorii cultivated by the method can bear 150-250 fruits, the fruit production of single plants of Siraitia grosvenorii is increased greatly, the yield per unit area of Siraitia grosvenorii is increased, planting benefit per unit area is increased, and cultivate area is reduced.

Description

A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique
Technical field
The present invention relates to the fruit tree planting technique field, particularly a kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique.
Background technology
Momordica grosvenori is a kind of perennial liana deciduous plant, and Dicotyledoneae, Cucurbitales, Curcurbitaceae, Momordica grosvenori belong to, and originate in the torrid zone, the Subtropical mountains that economize in Chinese Guangxi, Guangdong, Jiangxi etc., and be the most famous with Guangxi forest products Momordica grosvenori afterwards.Momordica grosvenori is except the fruit energy is medicinal, and grosvenor momordica leaf, Momordica grosvenori root also can be medicinal.Momordica grosvenori is the first batch of medicine-food two-purpose rare traditional Chinese medicine of announcing of the Ministry of Public Health, and sweet 300 times than sucrose of its contained Momordica-Glycosides do not produce heat, are the famous and precious raw materials of beverage, confectionery industry, are the best substitute of sucrose.Normal drink " Luohan " fruit tea can be prevented various diseases.Modern medicine proves that Momordica grosvenori has significant curative effect to diseases such as bronchitis, hypertension, can also play the effect that prevents and treats coronary heart disease, vascular sclerosis, obesity.
Usually, adopt the Momordica grosvenori traditional cultivation method, the fruit yield of individual plant Momordica grosvenori is about 60~80, adopt Momordica grosvenori standard culture technique, the fruit yield of individual plant Momordica grosvenori is about 80~100, and adopts high-yield culture technique, and the fruit yield of individual plant Momordica grosvenori is about 100~120.Existing market is large to the demand of Momordica grosvenori, and land resources is less, and the output of individual plant Momordica grosvenori still remains to be improved.
Summary of the invention
For defects, the characteristics that the present invention takes full advantage of plant can the term sorption nutrient, make nutrient by stages, supply nutrient by stages, be used for the cultivation Luohanguo With Plantlets of Tissue Culture, the fruit yield of every strain Momordica grosvenori can reach 150~250, increased substantially Luohanguo With Plantlets of Tissue Culture per plant quantity, improve Momordica grosvenori yield per unit area, reduced simultaneously unit Momordica grosvenori production cost, improved the unit are planting benefit.
The present invention is achieved by the following technical programs:
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique may further comprise the steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, without the soil of the pathogeny planting site as Luohanguo With Plantlets of Tissue Culture.
(2) in 3~April Luohanguo With Plantlets of Tissue Culture is colonizated in the planting site, spacing in the rows is 1.5~3 meters, and 3~6 jin base manure is executed in every strain, enriches 0.1~1 jin quick-acting composite fertilizers again; Base manure is pig, ox or the people's of becoming thoroughly decomposed ight soil.Can make the vegetative period maximization of nursery stock within year at field planting in 3~April Luohanguo With Plantlets of Tissue Culture.The growth of Momordica grosvenori seedling is higher to the demand of the fertilizer such as nitrogen, phosphorus, potassium early stage, enriches the demand that quick-acting composite fertilizers can satisfy the Momordica grosvenori seedling, and nursery stock is grown up healthy and sound quickly.
(3) at field scaffolding, when the Momordica grosvenori seedling grows to 15~25 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
When (4) treating that the Momordica grosvenori nursery stock grows to 50~150 centimetres, carry out lower pinch of top of the canopy first time, stay the upwards growth of a young shoot, execute 0.1~1 jin flower forcing fertilizer for simultaneously every strain Momordica grosvenori.Execute flower forcing fertilizer and can make that the secondary of nursery stock behind upper canopy is climing normally to be spent this moment.
(5) when the Momordica grosvenori nursery stock grows under the canopy 0~40 centimetre, carry out lower pinch of top of the canopy second time, stay canopy on two young shoots.Lower pinch of top of twice canopy can promote seedling root flourishing, and the blade face thickens, and the seedling stem increases slightly.
Behind the canopy, it is fixed into straight line toward left and right sides rightabout on (6) two young shoots, climing as one-level.
(7) every one-level is climing pinches the top when growing to 6~15 joint, and every climing 2~6 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 4~12 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as the first paragraph fruit bearing shoot, pinch the top when every fruit bearing shoot has 5~20 joint flower, every is stayed 5~30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the second segment fruit bearing shoot.
Pinch the top when (9) the second segment fruit bearing shoot has 5~20 joint flower, every is stayed 5~30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section fruit bearing shoot.
(10) reserve 4 sections~6 sections fruit bearing shoot by step (8), step (9), the final stage fruit bearing shoot is not pinched the top, is not removed bud, lets alone growth.
(11) at the setting of every section fruit bearing shoot during the phase, every strain Momordica grosvenori is used 0.1~1 jin of quick-acting composite fertilizer.The Momordica grosvenori fruiting period needs a large amount of liquid manure supplies, and the setting phase segmentation fertilising according to every section fruit bearing shoot can guarantee that Momordica grosvenori is to the demand of fertilizer.
(12) setting is during the phase, and final stage fruit bearing shoot fruit number is not controlled, and every section of other sections fruit bearing shoot stays 5~20 of fruits.
Beneficial effect of the present invention:
The present invention adopts the method for segmentation setting, make on every section fruit bearing shoot flowering time relatively concentrated, shorten that point on every section fruit bearing shoot is taken time and the time of Fruit, concentrate the fruit on liquid manure and each section of the nutrient supply fruit bearing shoot, effectively prolonged the setting phase of Momordica grosvenori, improved the fruiting quantities of individual plant Momordica grosvenori, increased substantially Momordica grosvenori yield per unit area, reduce simultaneously unit Momordica grosvenori production cost, improve the unit are planting benefit, reduce the cultivated land occupancy area.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited to the scope that embodiment represents.
Embodiment 1
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique may further comprise the steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, without the soil of the pathogeny planting site as Luohanguo With Plantlets of Tissue Culture.
(2) in March Luohanguo With Plantlets of Tissue Culture is colonizated in the planting site, spacing in the rows is 1.5 meters, and 3 jin base manure is executed in every strain, and base manure is the swine excrement that becomes thoroughly decomposed, and enriches 0.1 jin quick-acting composite fertilizers again.
(3) at field scaffolding, when the Momordica grosvenori seedling grows to 15 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
When (4) treating that the Momordica grosvenori nursery stock grows to 50 centimetres, carry out lower pinch of top of the canopy first time, stay the upwards growth of a young shoot, execute 0.1 jin flower forcing fertilizer for simultaneously every strain Momordica grosvenori.
(5) when the Momordica grosvenori nursery stock grows to lower 0 centimetre of canopy, carry out lower pinch of top of the canopy second time, stay canopy on two young shoots.
Behind the canopy, it is fixed into straight line toward left and right sides rightabout on (6) two young shoots, climing as one-level.
(7) every one-level is climing pinches the top when growing to 6 joint, and every climing 2 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 4 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as the first paragraph fruit bearing shoot, pinch the top when every fruit bearing shoot has 5 joint flower, every is stayed 5 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the second segment fruit bearing shoot.
Pinch the top when (9) the second segment fruit bearing shoot has 5 joint flower, every is stayed 5 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section fruit bearing shoot.
(10) reserve 4 sections fruit bearing shoot by step (8), step (9), the final stage fruit bearing shoot is not pinched the top, is not removed bud, lets alone growth.
(11) at the setting of every section fruit bearing shoot during the phase, every strain Momordica grosvenori is used 0.1 jin of quick-acting composite fertilizer.
(12) setting is during the phase, and final stage fruit bearing shoot fruit number is not controlled, and every section of other sections fruit bearing shoot stays 5 of fruits.
By this technology, during the Momordica grosvenori results, the fruit yield of every strain Momordica grosvenori is about 150.
Embodiment 2
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique may further comprise the steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, without the soil of the pathogeny planting site as Luohanguo With Plantlets of Tissue Culture.
(2) in April Luohanguo With Plantlets of Tissue Culture is colonizated in the planting site, spacing in the rows is 3 meters, and 6 jin base manure is executed in every strain, and base manure is the ight soil of the ox of becoming thoroughly decomposed, enriches 1 jin quick-acting composite fertilizers again.
(3) at field scaffolding, when the Momordica grosvenori seedling grows to 25 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
When (4) treating that the Momordica grosvenori nursery stock grows to 150 centimetres, carry out lower pinch of top of the canopy first time, stay the upwards growth of a young shoot, execute 1 jin flower forcing fertilizer for simultaneously every strain Momordica grosvenori.
(5) when the Momordica grosvenori nursery stock grows to lower 40 centimetres of canopy, carry out lower pinch of top of the canopy second time, stay canopy on two young shoots.
Behind the canopy, it is fixed into straight line toward left and right sides rightabout on (6) two young shoots, climing as one-level.
(7) every one-level is climing pinches the top when growing to 15 joint, and every climing 6 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 12 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as the first paragraph fruit bearing shoot, pinch the top when every fruit bearing shoot has 20 joint flower, every is stayed 30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the second segment fruit bearing shoot.
Pinch the top when (9) the second segment fruit bearing shoot has 20 joint flower, every is stayed 30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section fruit bearing shoot.
(10) reserve 6 sections fruit bearing shoot by step (8), step (9), the final stage fruit bearing shoot is not pinched the top, is not removed bud, lets alone growth.
(11) at the setting of every section fruit bearing shoot during the phase, every strain Momordica grosvenori is used 1 jin of quick-acting composite fertilizer.
(12) setting is during the phase, and final stage fruit bearing shoot fruit number is not controlled, and every section of other sections fruit bearing shoot stays 20 of fruits.
By this technology, during the Momordica grosvenori results, the fruit yield of every strain Momordica grosvenori is about 250.
Embodiment 3
A kind of Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique may further comprise the steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, without the soil of the pathogeny planting site as Luohanguo With Plantlets of Tissue Culture.
(2) in March Luohanguo With Plantlets of Tissue Culture is colonizated in the planting site, spacing in the rows is 2 meters, and 4 jin base manure is executed in every strain, and base manure is the people's of becoming thoroughly decomposed ight soil, enriches 0.5 jin quick-acting composite fertilizers again.
(3) at field scaffolding, when the Momordica grosvenori seedling grows to 20 centimetres, at the fixing Momordica grosvenori seedling of field inserted link.
When (4) treating that the Momordica grosvenori nursery stock grows to 100 centimetres, carry out lower pinch of top of the canopy first time, stay the upwards growth of a young shoot, execute 0.5 jin flower forcing fertilizer for simultaneously every strain Momordica grosvenori.
(5) when the Momordica grosvenori nursery stock grows to lower 20 centimetres of canopy, carry out lower pinch of top of the canopy second time, stay canopy on two young shoots.
Behind the canopy, it is fixed into straight line toward left and right sides rightabout on (6) two young shoots, climing as one-level.
(7) every one-level is climing pinches the top when growing to 10 joint, and every climing 4 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 8 climing as a result of branches of secondary.
(8) after fruit bearing shoot is spent, as the first paragraph fruit bearing shoot, pinch the top when every fruit bearing shoot has 10 joint flower, every is stayed 20 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the second segment fruit bearing shoot.
Pinch the top when (9) the second segment fruit bearing shoot has 10 joint flower, every is stayed 20 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section fruit bearing shoot.
(10) reserve 5 sections fruit bearing shoot by step (8), step (9), the final stage fruit bearing shoot is not pinched the top, is not removed bud, lets alone growth.
(11) at the setting of every section fruit bearing shoot during the phase, every strain Momordica grosvenori is used 0.5 jin of quick-acting composite fertilizer.
(12) setting is during the phase, and final stage fruit bearing shoot fruit number is not controlled, and every section of other sections fruit bearing shoot stays 10 of fruits.
By this technology, during the Momordica grosvenori results, the fruit yield of every strain Momordica grosvenori is about 200.

Claims (3)

1. Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique, it is characterized in that: this culture technique may further comprise the steps:
(1) select fertile soil, draining well, not ponding, have irrigation conditions, not arid, without the soil of the pathogeny planting site as Luohanguo With Plantlets of Tissue Culture;
(2) Luohanguo With Plantlets of Tissue Culture is colonizated in the planting site, spacing in the rows is 1.5~3 meters, and 3~6 jin base manure is executed in every strain, enriches 0.1~1 jin quick-acting composite fertilizers again;
(3) at field scaffolding, when the Momordica grosvenori seedling grows to 15~25 centimetres, at the fixing Momordica grosvenori seedling of field inserted link;
When (4) treating that the Momordica grosvenori nursery stock grows to 50~150 centimetres, carry out lower pinch of top of the canopy first time, stay the upwards growth of a young shoot, execute 0.1~1 jin flower forcing fertilizer for simultaneously every strain Momordica grosvenori;
(5) when the Momordica grosvenori nursery stock grows under the canopy 0~40 centimetre, carry out lower pinch of top of the canopy second time, stay canopy on two young shoots;
Behind the canopy, it is fixed into straight line toward left and right sides rightabout on (6) two young shoots, climing as one-level;
(7) every one-level is climing pinches the top when growing to 6~15 joint, and every climing 2~6 lateral buds of equidirectional that stay are climing as secondary, remove rightabout lateral bud; Every strain Momordica grosvenori stays 4~12 climing as a result of branches of secondary;
(8) after fruit bearing shoot is spent, as the first paragraph fruit bearing shoot, pinch the top when every fruit bearing shoot has 5~20 joint flower, every is stayed 5~30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the second segment fruit bearing shoot;
Pinch the top when (9) the second segment fruit bearing shoot has 5~20 joint flower, every is stayed 5~30 flowers, then removes lateral bud, and every fruit bearing shoot final section is deflorated and stayed lateral bud as the 3rd section fruit bearing shoot;
(10) reserve 4 sections~6 sections fruit bearing shoot by step (8), step (9), the final stage fruit bearing shoot is not pinched the top, is not removed bud, lets alone growth;
(11) at the setting of every section fruit bearing shoot during the phase, every strain Momordica grosvenori is used 0.1~1 jin of quick-acting composite fertilizer;
(12) setting is during the phase, and final stage fruit bearing shoot fruit number is not controlled, and every section of other sections fruit bearing shoot stays 5~20 of fruits;
2. Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique according to claim 1, it is characterized in that: the described Luohanguo With Plantlets of Tissue Culture definite value of step (2) was carried out in 3~April.
3. Luohanguo With Plantlets of Tissue Culture segmentation setting culture technique according to claim 2 is characterized in that: the described base manure of step (2) is pig, ox or the people's of becoming thoroughly decomposed ight soil.
CN201210330205.7A 2012-09-07 2012-09-07 Segmental-setting cultivation technique for Siraitia grosvenorii tissue culture seedlings Expired - Fee Related CN102845202B (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104322268A (en) * 2014-11-28 2015-02-04 陶进科 Momordica grosvenori mosaic virus avoiding method
CN104641858A (en) * 2014-06-10 2015-05-27 蒙柳宁 Fructus momordicae tissue culture seedling transplanting method
CN104904544A (en) * 2015-06-02 2015-09-16 广西大学 Segmented fruit-setting cultivation technique for passion fruit tissue culture seedlings
CN105103845A (en) * 2015-07-27 2015-12-02 广西鹿寨方田贸易有限公司 Momordica grosvenori cultivation method
CN106258736A (en) * 2016-08-12 2017-01-04 柳州市勤瑞农副产品有限公司 A kind of cultural method of Passifolra edulis tissue cultured seedling segmentation setting
CN109156285A (en) * 2018-08-24 2019-01-08 永福县农业局 Method for out-of-season cultivation of momordica grosvenori
CN111373996A (en) * 2020-04-20 2020-07-07 广西壮族自治区中国科学院广西植物研究所 High-yield cultivation method for momordica grosvenori

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CN101273691A (en) * 2008-05-19 2008-10-01 广西大学 Method for grafting Momordica grosvenori Swingle on gourd rootstock and cultivating seedling

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104641858A (en) * 2014-06-10 2015-05-27 蒙柳宁 Fructus momordicae tissue culture seedling transplanting method
CN104322268A (en) * 2014-11-28 2015-02-04 陶进科 Momordica grosvenori mosaic virus avoiding method
CN104904544A (en) * 2015-06-02 2015-09-16 广西大学 Segmented fruit-setting cultivation technique for passion fruit tissue culture seedlings
CN105103845A (en) * 2015-07-27 2015-12-02 广西鹿寨方田贸易有限公司 Momordica grosvenori cultivation method
CN106258736A (en) * 2016-08-12 2017-01-04 柳州市勤瑞农副产品有限公司 A kind of cultural method of Passifolra edulis tissue cultured seedling segmentation setting
CN109156285A (en) * 2018-08-24 2019-01-08 永福县农业局 Method for out-of-season cultivation of momordica grosvenori
CN111373996A (en) * 2020-04-20 2020-07-07 广西壮族自治区中国科学院广西植物研究所 High-yield cultivation method for momordica grosvenori

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