CN102753686B - Dna微阵列中的探针设计方法、具有利用该方法设计的探针的dna微阵列 - Google Patents

Dna微阵列中的探针设计方法、具有利用该方法设计的探针的dna微阵列 Download PDF

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Publication number
CN102753686B
CN102753686B CN201080063662.3A CN201080063662A CN102753686B CN 102753686 B CN102753686 B CN 102753686B CN 201080063662 A CN201080063662 A CN 201080063662A CN 102753686 B CN102753686 B CN 102753686B
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genomic dna
probe
restriction enzyme
dna fragment
design
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CN102753686A (zh
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榎宏征
西村哲
村上亚矢
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Toyota Motor Corp
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Toyota Motor Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201080063662.3A 2009-12-14 2010-12-13 Dna微阵列中的探针设计方法、具有利用该方法设计的探针的dna微阵列 Expired - Fee Related CN102753686B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009283430A JP5799484B2 (ja) 2009-12-14 2009-12-14 Ｄｎａマイクロアレイにおけるプローブ設計方法、当該方法により設計されたプローブを有するｄｎａマイクロアレイ
JP2009-283430 2009-12-14
PCT/JP2010/072322 WO2011074510A1 (ja) 2009-12-14 2010-12-13 Dnaマイクロアレイにおけるプローブ設計方法、当該方法により設計されたプローブを有するdnaマイクロアレイ

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CN102753686A CN102753686A (zh) 2012-10-24
CN102753686B true CN102753686B (zh) 2015-09-09

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US (2) US20120190582A1 (enExample)
EP (1) EP2514820B1 (enExample)
JP (1) JP5799484B2 (enExample)
CN (1) CN102753686B (enExample)
AU (1) AU2010331393B2 (enExample)
BR (1) BR112012014466B1 (enExample)
WO (1) WO2011074510A1 (enExample)

Families Citing this family (6)

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Publication number Priority date Publication date Assignee Title
JP5653957B2 (ja) * 2011-04-28 2015-01-14 トヨタ自動車株式会社 サトウキビ属植物の黒穂病抵抗性関連マーカーとその利用
JP6253132B2 (ja) 2013-09-09 2017-12-27 国立研究開発法人農業・食品産業技術総合研究機構 イチゴ属植物の炭疽病抵抗性関連マーカーとその利用
WO2017120750A1 (zh) * 2016-01-12 2017-07-20 中国科学院生物物理研究所 一种针对东亚人群全基因组范围内的非编码区的SNPs的DNA芯片
JP6515884B2 (ja) * 2016-06-29 2019-05-22 トヨタ自動車株式会社 Ｄｎａプローブの作製方法及びｄｎａプローブを用いたゲノムｄｎａ解析方法
WO2018053573A1 (en) * 2016-09-22 2018-03-29 Garvan Institute Of Medical Research Device for presenting sequencing data
JP7056012B2 (ja) 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1995384A (zh) * 2006-01-06 2007-07-11 王铸钢 一种快速方便转基因插入位点鉴定技术
CN101310024A (zh) * 2005-11-14 2008-11-19 科因股份有限公司 高通量筛选转座子标记群体和大量平行的插入位点的序列鉴定方法
CN101343667A (zh) * 2008-07-11 2009-01-14 中国水产科学研究院黄海水产研究所 一种水产动物snp标记筛选方法
CN101550434A (zh) * 2009-04-17 2009-10-07 中国海洋大学 一种条斑紫菜微卫星标记的筛选方法及其应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002528096A (ja) * 1998-10-27 2002-09-03 アフィメトリックス インコーポレイテッド ゲノムｄｎａの複雑性制御および分析
WO2000034518A1 (en) * 1998-12-04 2000-06-15 Keygene N.V. Array and method for analysing nucleic acid sequences
WO2000079006A1 (en) * 1999-06-17 2000-12-28 Fred Hutchinson Cancer Research Center Oligonucleotide arrays for high resolution hla typing
US6958225B2 (en) * 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
NZ521626A (en) 2000-03-29 2005-09-30 Cambia Methods for genotyping by hybridization analysis
US20030044791A1 (en) * 2001-06-13 2003-03-06 Flemington Erik Kolstad Adaptor kits and methods of use
US20030162181A1 (en) * 2002-02-28 2003-08-28 Eastman Kodak Company DNA sequencing and gene identification
EP1350853A1 (en) * 2002-04-05 2003-10-08 ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V. Detection of polymorphisms
JP2003330934A (ja) * 2002-05-10 2003-11-21 Celestar Lexico-Sciences Inc 変異体配列解析装置、変異体配列解析方法、プログラム、および、記録媒体
FR2853907B1 (fr) * 2003-04-18 2007-08-03 Commissariat Energie Atomique Procede de preparation de fragments d'adn par fragmentation selective d'acides nucleiques et ses applications
US7881875B2 (en) * 2004-01-16 2011-02-01 Affymetrix, Inc. Methods for selecting a collection of single nucleotide polymorphisms
DK2292788T3 (da) * 2005-06-23 2012-07-23 Keygene Nv Strategier til identifikation og detektion af polymorfismer med højt gennemløb
EP2363504A1 (en) * 2005-12-22 2011-09-07 Keygene N.V. Method for high-throughtput AFLP-based polymorphism detection
CN101096709B (zh) * 2006-06-28 2010-10-13 邓兴旺 利用可视薄膜感受器芯片检测特异核苷酸序列的方法
US8298768B2 (en) * 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
JP2009283430A (ja) 2008-05-19 2009-12-03 Dai Sasagawa バッテリー
CN101381724B (zh) * 2008-10-21 2010-09-01 中国科学院水生生物研究所 基于磁珠探针复合物分离短散在重复序列的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101310024A (zh) * 2005-11-14 2008-11-19 科因股份有限公司 高通量筛选转座子标记群体和大量平行的插入位点的序列鉴定方法
CN1995384A (zh) * 2006-01-06 2007-07-11 王铸钢 一种快速方便转基因插入位点鉴定技术
CN101343667A (zh) * 2008-07-11 2009-01-14 中国水产科学研究院黄海水产研究所 一种水产动物snp标记筛选方法
CN101550434A (zh) * 2009-04-17 2009-10-07 中国海洋大学 一种条斑紫菜微卫星标记的筛选方法及其应用

Also Published As

Publication number Publication date
US10214769B2 (en) 2019-02-26
AU2010331393B2 (en) 2014-06-19
JP2011120558A (ja) 2011-06-23
WO2011074510A1 (ja) 2011-06-23
EP2514820A1 (en) 2012-10-24
US20170166951A1 (en) 2017-06-15
BR112012014466B1 (pt) 2020-12-29
JP5799484B2 (ja) 2015-10-28
AU2010331393A1 (en) 2012-07-12
US20120190582A1 (en) 2012-07-26
CN102753686A (zh) 2012-10-24
EP2514820B1 (en) 2020-05-06
EP2514820A4 (en) 2013-08-07
BR112012014466A2 (pt) 2015-11-24

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