BR112012014466A2 - método para projetar sonda em microarranjos de dna, e microarranjos de dna providos com sonda projetada desse modo. - Google Patents

método para projetar sonda em microarranjos de dna, e microarranjos de dna providos com sonda projetada desse modo.

Info

Publication number
BR112012014466A2
BR112012014466A2 BR112012014466A BR112012014466A BR112012014466A2 BR 112012014466 A2 BR112012014466 A2 BR 112012014466A2 BR 112012014466 A BR112012014466 A BR 112012014466A BR 112012014466 A BR112012014466 A BR 112012014466A BR 112012014466 A2 BR112012014466 A2 BR 112012014466A2
Authority
BR
Brazil
Prior art keywords
probe
dna microarrays
way
designing
microarray
Prior art date
Application number
BR112012014466A
Other languages
English (en)
Other versions
BR112012014466B1 (pt
Inventor
Aya Murakami
Hiroyuki Enoki
Satoru Nishimura
Original Assignee
Toyota Motor Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyota Motor Co Ltd filed Critical Toyota Motor Co Ltd
Publication of BR112012014466A2 publication Critical patent/BR112012014466A2/pt
Publication of BR112012014466B1 publication Critical patent/BR112012014466B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

método para projetar sonda em microarranjo de dna, e microarranjo de dna provido com sonda projetada deste modo. a presente invenção refere-se a uma sonda a ser usada em um microarranjo de dna tendo uma excelente taxa de detecção de um polimorfismo tal como snp contido em dna genômico. um método para projetar uma sonda de acordo com a invenção inclui as etapas de: especificar uma ou mais regiões que cobrem pelo menos uma parte de fragmentos flanqueados pelos locais de reconhecimento da enzima de restrição reconhecidos por uma enzima de restrição, contida em dna genômico derivado de um organismo a ser testado; e projetar uma sonda para a especificada uma ou mais regiões para detecção do fragmento no organismo a ser testado.
BR112012014466-9A 2009-12-14 2010-12-13 método para detectar uma mutação usando um microarranjo de dna apresentando uma pluralidade de sondas de polinucleotídeo imobilizadas no mesmo BR112012014466B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009-283430 2009-12-14
JP2009283430A JP5799484B2 (ja) 2009-12-14 2009-12-14 Ｄｎａマイクロアレイにおけるプローブ設計方法、当該方法により設計されたプローブを有するｄｎａマイクロアレイ
PCT/JP2010/072322 WO2011074510A1 (ja) 2009-12-14 2010-12-13 Dnaマイクロアレイにおけるプローブ設計方法、当該方法により設計されたプローブを有するdnaマイクロアレイ

Publications (2)

Publication Number Publication Date
BR112012014466A2 true BR112012014466A2 (pt) 2015-11-24
BR112012014466B1 BR112012014466B1 (pt) 2020-12-29

Family

ID=44167263

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012014466-9A BR112012014466B1 (pt) 2009-12-14 2010-12-13 método para detectar uma mutação usando um microarranjo de dna apresentando uma pluralidade de sondas de polinucleotídeo imobilizadas no mesmo

Country Status (7)

Country Link
US (2) US20120190582A1 (pt)
EP (1) EP2514820B1 (pt)
JP (1) JP5799484B2 (pt)
CN (1) CN102753686B (pt)
AU (1) AU2010331393B2 (pt)
BR (1) BR112012014466B1 (pt)
WO (1) WO2011074510A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5653957B2 (ja) * 2011-04-28 2015-01-14 トヨタ自動車株式会社 サトウキビ属植物の黒穂病抵抗性関連マーカーとその利用
JP6253132B2 (ja) 2013-09-09 2017-12-27 国立研究開発法人農業・食品産業技術総合研究機構 イチゴ属植物の炭疽病抵抗性関連マーカーとその利用
CN107018668B (zh) * 2016-01-12 2018-07-10 中国科学院生物物理研究所 一种针对东亚人群全基因组范围内的非编码区的SNPs的DNA芯片
JP6515884B2 (ja) * 2016-06-29 2019-05-22 トヨタ自動車株式会社 Ｄｎａプローブの作製方法及びｄｎａプローブを用いたゲノムｄｎａ解析方法
WO2018053573A1 (en) * 2016-09-22 2018-03-29 Garvan Institute Of Medical Research Device for presenting sequencing data

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AU2144000A (en) * 1998-10-27 2000-05-15 Affymetrix, Inc. Complexity management and analysis of genomic dna
WO2000034518A1 (en) * 1998-12-04 2000-06-15 Keygene N.V. Array and method for analysing nucleic acid sequences
WO2000079006A1 (en) * 1999-06-17 2000-12-28 Fred Hutchinson Cancer Research Center Oligonucleotide arrays for high resolution hla typing
US6958225B2 (en) * 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
WO2001073119A2 (en) 2000-03-29 2001-10-04 Cambia Methods for genotyping by hybridization analysis
US20030044791A1 (en) * 2001-06-13 2003-03-06 Flemington Erik Kolstad Adaptor kits and methods of use
US20030162181A1 (en) * 2002-02-28 2003-08-28 Eastman Kodak Company DNA sequencing and gene identification
EP1350853A1 (en) * 2002-04-05 2003-10-08 ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V. Detection of polymorphisms
JP2003330934A (ja) * 2002-05-10 2003-11-21 Celestar Lexico-Sciences Inc 変異体配列解析装置、変異体配列解析方法、プログラム、および、記録媒体
FR2853907B1 (fr) * 2003-04-18 2007-08-03 Commissariat Energie Atomique Procede de preparation de fragments d'adn par fragmentation selective d'acides nucleiques et ses applications
US7881875B2 (en) * 2004-01-16 2011-02-01 Affymetrix, Inc. Methods for selecting a collection of single nucleotide polymorphisms
ES2387878T3 (es) * 2005-06-23 2012-10-03 Keygene N.V. Estrategias para la identificación de alto rendimiento y la detección de polimorfismos
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CN101343667A (zh) * 2008-07-11 2009-01-14 中国水产科学研究院黄海水产研究所 一种水产动物snp标记筛选方法
CN101381724B (zh) * 2008-10-21 2010-09-01 中国科学院水生生物研究所 基于磁珠探针复合物分离短散在重复序列的方法
CN101550434B (zh) * 2009-04-17 2011-10-26 中国海洋大学 一种条斑紫菜微卫星标记的筛选方法及其应用

Also Published As

Publication number Publication date
CN102753686A (zh) 2012-10-24
AU2010331393A1 (en) 2012-07-12
US20120190582A1 (en) 2012-07-26
EP2514820A1 (en) 2012-10-24
JP5799484B2 (ja) 2015-10-28
CN102753686B (zh) 2015-09-09
US20170166951A1 (en) 2017-06-15
JP2011120558A (ja) 2011-06-23
US10214769B2 (en) 2019-02-26
BR112012014466B1 (pt) 2020-12-29
AU2010331393B2 (en) 2014-06-19
WO2011074510A1 (ja) 2011-06-23
EP2514820A4 (en) 2013-08-07
EP2514820B1 (en) 2020-05-06

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

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B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2753 DE 10-10-2023 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.