CN102630492A - Production method for cultivating gingko agaric by utilizing medicinal plant residues - Google Patents
Production method for cultivating gingko agaric by utilizing medicinal plant residues Download PDFInfo
- Publication number
- CN102630492A CN102630492A CN201210118510XA CN201210118510A CN102630492A CN 102630492 A CN102630492 A CN 102630492A CN 201210118510X A CN201210118510X A CN 201210118510XA CN 201210118510 A CN201210118510 A CN 201210118510A CN 102630492 A CN102630492 A CN 102630492A
- Authority
- CN
- China
- Prior art keywords
- leaf
- ginkgo
- stem
- root
- red sage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention provides a production method for cultivating gingko agaric by utilizing medicinal plant residues. The production method comprises the steps of firstly collecting ginkgo leaves, gingko branch wood, red sage root stem leaves, honeysuckle stalks, schisandra chinensis rhizome leaves and astragalus stem leaves at the right time; drying and smashing the collected raw materials for later use; finally preparing a culture medium for cultivating the agaric by using the smashed raw materials according to a certain proportion and managing according to conventional methods of bagging, sterilization, cooling, inoculation, cultivation, agaric accelerating and harvesting, wherein an extract liquid of the ginkgo leaves, the honeysuckle stalks, the red sage root stem leaves, the schisandra chinensis rhizome leaves and the astragalus stem leaves is added in the cultivating process. The production method provided by the invention has the advantages that residue precious resources of rare and valuable Chinese herbal medicines, such as the gingko branch wood, the ginkgo leaves, red sage root, schisandra chinensis, astragalus root, honeysuckle and the like, are fully utilized; a new way of comprehensively utilizing the residue resources of medicinal plants is developed; a novel green healthy food is provided for people; and the dependence to woods and the damage to forest of agaric production are reduced.
Description
Technical field:
The present invention relates to the production method of a kind of new function type edible mushroom; Particularly a kind of is main raw material(s) with medicinal plant residue ginkgo leaf, ginkgo brush wood, is a kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae of auxiliary material with red sage root cauline leaf, honeysuckle stalk, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf etc.
Background technology:
China has become Edible Fungi big country, and the bright article annual production of edible mushroom has reached more than 1,000 ten thousand tons, accounts for 2/3 of world's total amount.Edible Fungi has become the rural area three big industries of laying equal stress on plant husbandry, aquaculture, is the important channel of China mountain area or poverty-stricken area peasant programme.Yet, exist serious phenomenons such as forest tree resource consumption is big, product function is single, economic benefit is low, the reversal of the natural order of things of output ratio in China's Edible Fungi; Mushroom industry has stepped into the vicious circle of piecing together resource, assembly consumption, low output in fact.It is reported that the forest consumption that China in 2006 is used for " the livings bacterium of wood " production reaches 3,800 ten thousand m
3, caused serious forest tree resource felling, the destruction and the secondary disaster of ecotope to take place frequently, " bacterium woods contradiction " becomes increasingly conspicuous.Along with the mushroom industry development, utilize novel culture medium, creative development function type edible mushroom, the added value of raising edible fungus has become the problem of being badly in need of exploration and solving.
Cultivating in bag is the last great technological innovation of Edible Fungi history, and it has shortened the Edible Fungi cycle, has increased substantially the biology conversion ratio.Yet for a long time, edible fungus in bags production is many to be raw material with farming, forest by-product stalk, cotton seed hull, wood chip, vinasse, straw etc., and the edible mushroom gap that product quality and oak are produced is bigger.For addressing this problem, relevant scholar utilizes abundant pruning fruit tree brush wood to be resource, has carried out edible fungus culturing tests such as Bag Material mushroom, auricularia auriculajudae, has obtained remarkable economic efficiency.But, utilize the medicinal plant residue but to rarely have report for raw material production functional form edible mushroom.Medicinal plant is the important component part of China's traditional Chinese medicine, and China is medicinal plant plantation in the world and the maximum country of consumption, for China's traditional Chinese medicine industrial expansion and the development and use of medicinal plant residue, sufficient raw material is provided.Up to the present, these medicinal plant residues are the overwhelming majority go out of use, and reasonably do not developed, and caused huge waste; These medicinal plant residues contain the abundant active ingredient that helps health a bit.In recent years, the report of some residues that utilize medicinal plant for raw material production functional form auricularia auriculajudae also arranged.Like publication number CN101642030, name is called a kind of cultivation method and culture medium for cultivating thereof that is rich in the Hippophate flavone woodear, and it adds the sea-buckthorn slag in medium, produced a kind of woodear that is rich in Hippophate flavone, has increased the health-care efficacy of woodear.Publication No. CN102204473A; Name is called the method with the medium cultivation edibility mushroom that contains kiwi fruit branch/limb composition; It has cultivated auricularia auriculajudae etc. with the medium that contains the dry kiwi fruit branch wood pellet that accounts for nutrient component gross weight 10% ~ 90%; Make the discarded limb of resource value be able to utilize fully again, turn waste into wealth, increase substantially the output of edibility mushroom.Remaining some patent documentation also discloses and has utilized ramulus mori, red sage root dis-medicinal part, balloonflower root dis-medicinal part, chrysanthemum dis-medicinal part and honeysuckle rhizome etc. to cultivate auricularia auriculajudae for raw material.Ginkgo leaf is rich in the flavonoids that improves the cardiovascular system of human body function.The inventor was former, auxiliary material with ginkgo brush wood, ginkgo leaf, honeysuckle, the red sage root etc. once; Invented a kind of breeding method of ginkgo mushroom; The patent No. is ZL2011100562170; For the utilization of ginkgo medicine residue provides new evolutionary path, but owing to contained medicinal materials such as Schisandra sphenanthera Rehd.et WilS, the Radix Astragali in the former medium, make that to cultivate composition somewhat expensive.And their some effective compositions also can not get sufficient utilization.
Summary of the invention:
In sum, in order to alleviate " the bacterium woods contradiction " that becomes increasingly conspicuous, science, reasonably utilize the medicinal plant residue, solve the technical problem that exists in the actual production.The present invention has developed a kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae.It is to the outstanding problem that exists in the Edible Fungi; With ginkgo brush wood, ginkgo leaf is main raw material(s); With red sage root cauline leaf, honeysuckle stalk, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf etc. is auxiliary material, a kind of new function type edible mushroom " ginkgo auricularia auriculajudae " that human body is had more health of cultivating.For the research and development that utilize the medicinal plant residue to cultivate the functional form edible mushroom provide highly effective approach and research thinking.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae, this method may further comprise the steps:
The first step, culture medium raw material collection: the late October ~ collect ginkgo brush wood and the ginkgo leaf prune mid-January; Pluck ginkgo leaf in mid-June ~ mid-September; The early June ~ early August collects the honeysuckle stalk; Collect red sage root cauline leaf in early August ~ mid-September, collect the Schisandra sphenanthera Rehd.et WilS root, stem and leaf in September, the late August ~ early October collects membranous milkvetch stem and leaf;
Second step, culture medium raw material preparation, storage: ginkgo leaf, ginkgo brush wood, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, the membranous milkvetch stem and leaf gathered are dried to moisture content 6.0% ~ 14.5%; Be ground into the particle of 0.5mm ~ 2.5mm respectively, place drying, shady and cool place to store;
The 3rd step, preparing culture medium: will pass through the culture medium raw material of pulverizing, according to weight percent meter, ginkgo leaf 10%～30%; Ginkgo brush wood 10% ~ 45%, red sage root cauline leaf 5%～10%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5% ~ 10%, membranous milkvetch stem and leaf 5% ~ 10%; Honeysuckle stalk 5% ~ 10%, wheat bran 15～25%, sucrose 1～1.5%; Land plaster 1～2%, broadleaf wood chip 5% ~ 30% is configured to medium; Then with the medium that configures according to weight ratio; Medium: water: ethanol=1: (1.0～1.5): (0.003～0.05), medium, clear water and ethanol are mixed, manage according to the conventional method that packs, sterilizes, cools off, inoculates, cultivates, urges ear, gather at last;
The 4th step, leaching liquor preparation: before urging ear 3 ~ 15 days; Particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf pulverized is with (2 ~ 7): (0.5～2): (0.5～2): (0.5～2): the weight ratio of (0.5～2) mixes, mixture, the weight ratio according to mixture and clear water is (1:3) ~ (1:40) mixed then; Mix back by clear water: the weight ratio of ethanol is 1: (0.001 ~ 0.05); Add ethanol and stir, be heated to 65～78 ℃ immediately, continue insulation after 30 ~ 120 minutes; Naturally cooling is crossed and is filtered leaching liquor;
The 5th step, leaching liquor inject: will filter and cooled leaching liquor injected the bacterium bag in 12 hours, every bag at every turn the amount of injection leaching liquor be 0.15 ~ 1.5kg; Or when urging ear between picking time, to need to replenish moisture content, substitute clear water with leaching liquor and replenish.
Further, described ethanol is that 95% food is used ethanol.
Further, described leaching liquor preparation is that the particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf are pulverized is (2 ~ 7) according to weight ratio: (0.5～2): (0.5～2): (0.5～2): (0.5～2), each raw material is mixed; Mixture, then with the clear water of mixture and 40 ℃ ~ 60 ℃ according to weight ratio (1:3) ~ (1:40) mixed, and by clear water: the weight ratio of ethanol is 1: (0.001 ~ 0.05); Adding ethanol stirs; Soak after 30～60 minutes, be heated to 65～78 ℃ immediately, continue insulation after 30 ~ 120 minutes; To cooling naturally, cross and filter leaching liquor.
The ginkgo auricularia auriculajudae that the present invention cultivates has made full use of precious resources such as rare Chinese medicine plant residue ginkgo brush wood, ginkgo leaf, red sage root cauline leaf, honeysuckle stalk, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf; Opened up the new way of ginkgo comprehensive utilization; To people a kind of novel green health food is provided; Promoted the beneficial functions of the auricularia auriculajudae of traditional cultivation mode to the health guarantee; The cultivating and growing that has reduced auricularia auriculajudae is new road of market development of China's pollution-free food, healthy food to the dependence of forest with to the destruction of forest; Ginkgo brush wood, ginkgo leaf contain flavonoid glycoside; The Schisandra sphenanthera Rehd.et WilS root, stem and leaf is warm in nature, and flavor acid, sweet has convergence astringent or styptic treatment for spontaneous sweating, nourishing generate fluid, the function of kidney calming; Membranous milkvetch stem and leaf is warm in nature, and it is sweet to distinguish the flavor of, and invigorating qi for strengthening superficies is arranged, diuresis detoxification, apocenosis, the function of expelling pus and promoting granulation; The efficacy component of honeysuckle stalk mainly is a chlorogenic acid, and chlorogenic acid contents is up to 2.33 in the honeysuckle, and honeysuckle is one of definite rare traditional Chinese medicine of State Council, is the choice drug of treatment bird flu and Influenza A H1N1; The functional form ginkgo auricularia auriculajudae that the present invention utilizes ginkgo plant residue to cultivate for primary raw material contains the active component that helps health---the GINKGO BILOBA EXTRACT class.
Good effect of the present invention is:
1,, effectively utilizes famous and precious medicinal plant residues such as ginkgo leaf, ginkgo brush wood, red sage root cauline leaf, honeysuckle stalk, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf, for the exploitation of medicinal plant residue provides scientific basis through enforcement of the present invention.
2, the present invention cultivates the succeeding in developing of production method of ginkgo auricularia auriculajudae; Residue precious resources such as rare traditional Chinese medicine ginkgo brush wood, ginkgo leaf, the red sage root, Schisandra sphenanthera Rehd.et WilS, astragalus root, honeysuckle have been made full use of; Opened up the new way of medicinal plant residue resource comprehensive utilization; To people a kind of novel green health food is provided, has reduced auricularia auriculajudae and produced to the dependence of forest with to the destruction of forest.New road of market development for China's pollution-free food, healthy food.
3, to cultivate the production method of ginkgo auricularia auriculajudae be in medium and leaching liquor thereof, to have added ethanol in the present invention; The more affine ethanol of GINKGO BILOBA EXTRACT class; Beneficiating ingredient in the medicine residues such as the ginkgo leaf that makes dissolves in water more easily; Absorbed by auricularia auriculajudae, the active component that helps health in the ginkgo auricularia auriculajudae of therefore cultivating is more, and for example dry measure used in former times skin content is (mass fraction) 0.0083～0.049 ‰; The kaempferia galamga cellulose content is (mass fraction) 0.011～0.049 ‰; Total aglycon content content is (mass fraction) 0.019～0.087 ‰; Total flavonoids content is (mass fraction) 0.048～0.22 ‰, for the research and development of functional edible mushroom provide highly effective approach and research thinking.
Embodiment:
Below in conjunction with embodiment the present invention is done further detailed description.
Embodiment 1:
A kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae, this method may further comprise the steps:
The first step, culture medium raw material collection: at ginkgo brush wood and the ginkgo leaf on annual November, under the collection pruning in the middle ten days; In 6 months, pluck ginkgo leaf the last ten-days period; Collected the honeysuckle stalk on June 10; Collected red sage root cauline leaf on September 5, collected the Schisandra sphenanthera Rehd.et WilS root, stem and leaf on September 10, September 2, part was collected membranous milkvetch stem and leaf;
Second step, culture medium raw material preparation, storage: ginkgo leaf, ginkgo brush wood, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, the membranous milkvetch stem and leaf gathered are dried to moisture content 8.5%; Be ground into the particle of 0.5mm respectively, place drying, shady and cool place to store;
The 3rd step, preparing culture medium: according to weight percent meter, will pass through the culture medium raw material of pulverizing, ginkgo leaf 10%, ginkgo brush wood 43%; Red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%, membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%; Broadleaf wood chip 5%, wheat bran 20%, sucrose 1%; Land plaster 1% is configured to medium, then with the medium that configures according to weight ratio; Medium: water: ethanol (95% edible ethanol)=1:1.1:0.003, medium is mixed, manage according to the conventional method that packs, sterilizes, cools off, inoculates, cultivates, urges ear, gather;
The 4th step, leaching liquor preparation: urging ear preceding 3 days; The part by weight of particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, the membranous milkvetch stem and leaf pulverizing with 6:1:1:1:1 mixed; Get mixture, the weight ratio according to mixture and clear water is the 1:15 mixed then, and mix back by clear water: the weight ratio of 95% edible ethanol is 1:0.02; Add edible ethanol and be heated to 65～78 ℃ immediately; Continue insulation after 60 minutes, cooling is naturally crossed and is filtered leaching liquor;
The 5th step, leaching liquor inject: will filter and cooled leaching liquor injected the bacterium bag in 12 hours, every bag at every turn the amount of injection leaching liquor be 0.5kg; Afterwards, from urging ear, whenever injected the 0.5kg leaching liquor to the bacterium bag once more at a distance from 5 days to picking time.
Embodiment 2:
A kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae, this method may further comprise the steps:
The first step, culture medium raw material collection: collect ginkgo brush wood and ginkgo leaf under pruning in annual late October; Pluck ginkgo leaf mid-August, collected the honeysuckle stalk on July 10, collected red sage root cauline leaf on August 5; Collect the Schisandra sphenanthera Rehd.et WilS root, stem and leaf on September 15, collected membranous milkvetch stem and leaf on August 28;
Second step, culture medium raw material preparation, storage: ginkgo leaf, ginkgo brush wood, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, the membranous milkvetch stem and leaf gathered are dried to moisture content 9.0%; Be ground into the particle of 0.8mm respectively, place drying, shady and cool place to store;
The 3rd step, preparing culture medium: according to weight percent meter, will pass through the culture medium raw material of pulverizing, ginkgo leaf 20%, ginkgo brush wood 30%; Red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%, membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%; Broadleaf wood chip 13%, wheat bran 15%, sucrose 1%; Land plaster 1% is configured to medium, then with the medium that configures according to weight ratio; Medium: water: ethanol (95% edible ethanol)=1:1.2:0.01, medium is mixed, manage according to the conventional method that packs, sterilizes, cools off, inoculates, cultivates, urges ear, gather;
The 4th step, leaching liquor preparation: urging ear preceding 5 days; The part by weight of particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, the membranous milkvetch stem and leaf pulverizing with 7:0.5:1:1:0.5 mixed, get mixture, the weight ratio according to mixture and clear water is the 1:10 mixed then; Mix back by clear water: the weight ratio of 95% edible ethanol is 1:0.01; Add 95% edible ethanol and stir, be heated to 65～78 ℃ immediately, continue insulation after 90 minutes; Naturally cooling is crossed and is filtered leaching liquor;
The 5th step, leaching liquor inject: will filter and cooled leaching liquor injected the bacterium bag in 6 hours, every bag at every turn the amount of injection leaching liquor be 0.8kg; Afterwards, from urging ear, whenever injected the 0.8kg leaching liquor to the bacterium bag once more at a distance from 8 days to picking time.
Embodiment 3:
A kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae, this method may further comprise the steps:
The first step, culture medium raw material collection: collect ginkgo brush wood and ginkgo leaf under pruning in annual mid-December; Pluck ginkgo leaf in mid-July, collected the honeysuckle stalk on June 1, collected red sage root cauline leaf on August 25; Collect the Schisandra sphenanthera Rehd.et WilS root, stem and leaf on September 25, collected membranous milkvetch stem and leaf on September 15;
Second step, culture medium raw material preparation, storage: ginkgo leaf, ginkgo brush wood, honeysuckle stalk, Schisandra sphenanthera Rehd.et WilS, the astragalus root gathered are dried to moisture content 11.0%, be ground into the particle of 1.2mm respectively; Place drying, shady and cool place to store;
The 3rd step, preparing culture medium: according to weight percent meter, will pass through the culture medium raw material of pulverizing, ginkgo leaf 30%, ginkgo brush wood 16.5%; Red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%, membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%; Broadleaf wood chip 5%, wheat bran 25%, sucrose 1.5%; Land plaster 2% is configured to medium, then with the medium that configures according to weight ratio; Medium: water: the ratio of ethanol (95% edible ethanol)=1:1.3:0.02, medium is mixed, manage according to the conventional method that packs, sterilizes, cools off, inoculates, cultivates, urges ear, gather;
The 4th step, leaching liquor preparation: urging ear preceding 10 days, the particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf are pulverized mixes with the part by weight of 5:1.5:1:1:1.5, gets mixture; Weight ratio according to mixture and 40 ℃ ~ 60 ℃ of clear water is the 1:20 mixed then; Mix back by clear water: the weight ratio of 95% edible ethanol is 1:0.02, adds 95% edible ethanol and stirs, and soaks after 60 minutes; Be heated to 65～78 ℃ immediately; Continue insulation after 60 minutes, cooling is naturally crossed and is filtered leaching liquor;
The 5th step, leaching liquor inject: will filter and cooled leaching liquor injected the bacterium bag in 3 hours, every bag at every turn the amount of injection leaching liquor be 1.5kg; Afterwards, from urging ear, whenever injected the 1.0kg leaching liquor to the bacterium bag once more at a distance from 10 days to picking time.
Embodiment 4:
A kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae, this method may further comprise the steps:
The first step, culture medium raw material collection: collect ginkgo brush wood and ginkgo leaf under pruning in annual late December; Early September is plucked ginkgo leaf, and early July is collected the honeysuckle stalk, and late August is collected red sage root cauline leaf; Collect the Schisandra sphenanthera Rehd.et WilS root, stem and leaf mid-September, late September is collected membranous milkvetch stem and leaf;
Second step, culture medium raw material preparation, storage: ginkgo leaf, ginkgo brush wood, honeysuckle stalk, Schisandra sphenanthera Rehd.et WilS, the astragalus root gathered are dried to moisture content 13.0%, be ground into the particle of 2.0mm respectively; Place drying, shady and cool place to store;
The 3rd step, preparing culture medium: according to weight percent meter, will pass through the culture medium raw material of pulverizing, ginkgo leaf 11.5%, ginkgo brush wood 10%; Red sage root cauline leaf 10%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 10%, membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%; Broadleaf wood chip 30%, wheat bran 15%, sucrose 1.5%; Land plaster 2% is configured to medium, then with the medium that configures according to weight ratio; Medium: water: ethanol (95% edible ethanol)=1:1.4:0.03, medium is mixed, manage according to the conventional method that packs, sterilizes, cools off, inoculates, cultivates, urges ear, gather;
The 4th step, leaching liquor preparation: urging ear preceding 10 days, the particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf are pulverized mixes with the weight ratio of 4:1.5:1.5:1.5:1.5, gets mixture; Be that 1:30 mixes according to mixture with the weight ratio of 40 ℃ ~ 60 ℃ of clear water then; Mix back by clear water: the weight ratio of 95% edible ethanol is 1:0.03, adds 95% edible ethanol and stirs, and soaks after 60 minutes; Be heated to 65～78 ℃ immediately; Continue insulation after 60 minutes, cooling is naturally crossed and is filtered leaching liquor;
The 5th step, leaching liquor inject: will filter and cooled leaching liquor injected the bacterium bag in 6 hours, every bag at every turn the amount of injection leaching liquor be 1.5kg; Afterwards, from urging ear, whenever injected the 1.5kg leaching liquor to the bacterium bag once more at a distance from 8 days to picking time.
Embodiment 5:
A kind of production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae, this method may further comprise the steps:
The first step, culture medium raw material collection: collect ginkgo brush wood and ginkgo leaf under pruning in annual mid-January; Pluck ginkgo leaf mid-September, early August is collected the honeysuckle stalk, collects the red sage root cauline leaf mid-September; Late September is collected the Schisandra sphenanthera Rehd.et WilS root, stem and leaf, and early October is collected membranous milkvetch stem and leaf;
Second step, culture medium raw material preparation, storage: ginkgo leaf, ginkgo brush wood, honeysuckle stalk, Schisandra sphenanthera Rehd.et WilS, the astragalus root gathered are dried to moisture content 14.0%, be ground into the particle of 2.5mm respectively; Place drying, shady and cool place to store;
The 3rd step, preparing culture medium: according to weight percent meter, will pass through the culture medium raw material of pulverizing, ginkgo leaf 11.5%, ginkgo brush wood 20%; Red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%, membranous milkvetch stem and leaf 10%, honeysuckle stalk 10%; Broadleaf wood chip 20%, wheat bran 15%, sucrose 1.5%; Land plaster 2% is configured to medium, then with the medium that configures according to weight ratio; Medium: water: ethanol (95% edible ethanol)=1:1.5:0.05, medium is mixed, manage according to the conventional method that packs, sterilizes, cools off, inoculates, cultivates, urges ear, gather;
The 4th step, leaching liquor preparation: urging ear preceding 10 days, the particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf are pulverized mixes with the weight ratio of 4:1:2:1:2, gets mixture; Mix according to the weight ratio 1:50 of mixture then with 40 ℃ ~ 60 ℃ of clear water; Mix back by clear water: the weight ratio of 95% edible ethanol is 1:0.03, adds 95% edible ethanol and stirs, and soaks after 60 minutes; Be heated to 65～78 ℃ immediately; Continue insulation after 60 minutes, cooling is naturally crossed and is filtered leaching liquor;
The 5th step, leaching liquor inject: will filter and cooled leaching liquor injected the bacterium bag in 6 hours, every bag at every turn the amount of injection leaching liquor be 1.5kg; Afterwards, when urging ear to picking time, need to replenish moisture content, substitute clear water with leaching liquor and replenish.
Claims (8)
1. production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae is characterized in that this method may further comprise the steps:
The first step, culture medium raw material collection: the late October～ginkgo brush wood and ginkgo leaf under collect pruning mid-January; Pluck ginkgo leaf in mid-June～mid-September; The early June～early August collects the honeysuckle stalk; Collect red sage root cauline leaf in early August～mid-September, collect the Schisandra sphenanthera Rehd.et WilS root, stem and leaf in September, the late August～early October collects membranous milkvetch stem and leaf;
Second step, culture medium raw material preparation, storage: ginkgo leaf, ginkgo brush wood, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, the membranous milkvetch stem and leaf gathered are dried to moisture content 6.0%～14.5%; Be ground into the particle of 0.5mm～2.5mm respectively, place drying, shady and cool place to store;
The 3rd step, preparing culture medium: will pass through the culture medium raw material of pulverizing, according to weight percent meter, ginkgo leaf 10%～30%; Ginkgo brush wood 10%～45%, red sage root cauline leaf 5%～10%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%～10%, membranous milkvetch stem and leaf 5%～10%; Honeysuckle stalk 5%～10%, wheat bran 15～25%, sucrose 1～1.5%; Land plaster 1～2%, broadleaf wood chip 5%～30% is configured to medium; Then with the medium that configures according to weight ratio; Medium: water: ethanol=1:1.0～1.5:0.003～0.05 mixes medium, clear water and ethanol, manages according to the conventional method that packs, sterilizes, cools off, inoculates, cultivates, urges ear, gather at last;
The 4th step, leaching liquor preparation: before urging ear 3～15 days; The weight ratio of particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, the membranous milkvetch stem and leaf pulverizing with 2～7:0.5～2:0.5～2:0.5～2:0.5～2 mixed, get mixture, the weight ratio according to mixture and clear water is 1:3～1:40 mixed then; Mix back by clear water: the weight ratio of ethanol is 1:0.001～0.05; Add ethanol and stir, be heated to 65～78 ℃ immediately, continue insulation after 30～120 minutes; Naturally cooling is crossed and is filtered leaching liquor;
The 5th step, leaching liquor inject: will filter and cooled leaching liquor injected the bacterium bag in 12 hours, every bag at every turn the amount of injection leaching liquor be 0.15～1.5kg; Or when urging ear between picking time, to need to replenish moisture content, substitute clear water with leaching liquor and replenish.
2. the production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae according to claim 1 is characterized in that: described ethanol is that 95% food is used ethanol.
3. the production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae according to claim 1; It is characterized in that: described leaching liquor preparation is that the particle after ginkgo leaf, honeysuckle stalk, red sage root cauline leaf, Schisandra sphenanthera Rehd.et WilS root, stem and leaf, membranous milkvetch stem and leaf are pulverized mixes each raw material according to weight ratio 2～7:0.5～2:0.5～2:0.5～2:0.5～2; Mixture, then with the clear water of mixture and 40 ℃～60 ℃ according to weight ratio 1:3～1:40 mixed, and by clear water: the weight ratio of ethanol is 1:0.001～0.05; Adding ethanol stirs; Soak after 30～60 minutes, be heated to 65～78 ℃ immediately, continue insulation after 30～120 minutes; To cooling naturally, cross and filter leaching liquor.
4. the production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae according to claim 1 is characterized in that: the medium in described the 3rd step is according to the weight ratio meter, by ginkgo leaf 10%; Ginkgo brush wood 43%, red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%; Membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%, broadleaf wood chip 5%; Wheat bran 20%, sucrose 1%, land plaster 1% is formed.
5. the production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae according to claim 1 is characterized in that: the medium in described the 3rd step is according to the weight ratio meter, by ginkgo leaf 20%; Ginkgo brush wood 30%, red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%; Membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%, broadleaf wood chip 13%; Wheat bran 15%, sucrose 1%, land plaster 1% is formed.
6. the production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae according to claim 1 is characterized in that: the medium in described the 3rd step is according to the weight ratio meter, by ginkgo leaf 30%; Ginkgo brush wood 16.5%, red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%; Membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%, broadleaf wood chip 5%; Wheat bran 25%, sucrose 1.5%, land plaster 2% is formed.
7. the production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae according to claim 1 is characterized in that: the medium in described the 3rd step is according to the weight ratio meter, by ginkgo leaf 11.5%; Ginkgo brush wood 10%, red sage root cauline leaf 10%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 10%; Membranous milkvetch stem and leaf 5%, honeysuckle stalk 5%, broadleaf wood chip 30%; Wheat bran 15%, sucrose 1.5%, land plaster 2% is formed.
8. the production method of utilizing the medicinal plant residue to cultivate the ginkgo auricularia auriculajudae according to claim 1 is characterized in that: the medium in described the 3rd step is according to the weight ratio meter, by ginkgo leaf 11.5%; Ginkgo brush wood 20%, red sage root cauline leaf 5%, Schisandra sphenanthera Rehd.et WilS root, stem and leaf 5%; Membranous milkvetch stem and leaf 10%, honeysuckle stalk 10%, broadleaf wood chip 20%; Wheat bran 15%, sucrose 1.5%, land plaster 2% is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210118510 CN102630492B (en) | 2012-04-23 | 2012-04-23 | Production method for cultivating gingko agaric by utilizing medicinal plant residues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210118510 CN102630492B (en) | 2012-04-23 | 2012-04-23 | Production method for cultivating gingko agaric by utilizing medicinal plant residues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102630492A true CN102630492A (en) | 2012-08-15 |
| CN102630492B CN102630492B (en) | 2013-05-22 |
Family
ID=46615210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210118510 Expired - Fee Related CN102630492B (en) | 2012-04-23 | 2012-04-23 | Production method for cultivating gingko agaric by utilizing medicinal plant residues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102630492B (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104115671A (en) * | 2014-07-18 | 2014-10-29 | 国家林业局泡桐研究开发中心 | Dogwood lentinula edodes cultivating method |
| CN104311284A (en) * | 2014-09-26 | 2015-01-28 | 侯中仁 | Preparation method of black fungus culture medium |
| CN105123401A (en) * | 2015-08-25 | 2015-12-09 | 全椒林农生态农业科技有限公司 | Method for interplanting apocarya with salvia miltiorrhiza |
| CN105766377A (en) * | 2016-04-19 | 2016-07-20 | 牡丹江师范学院 | Culture method capable of increasing the flavonoid content and varieties of auricularia auricula |
| CN106365831A (en) * | 2016-08-30 | 2017-02-01 | 杭州云开生态农业开发有限公司 | Auricularia auricula culture medium and preparation method thereof |
| CN106818116A (en) * | 2016-12-20 | 2017-06-13 | 长兴众化生物科技有限公司 | A kind of method that FLOS CHRYSANTHEMI ALBA from Haizhou of China is planted in Zhejiang area salt-soda soil |
| CN107176872A (en) * | 2017-02-10 | 2017-09-19 | 邢建国 | A kind of production method of feature agaric |
| CN107347452A (en) * | 2017-08-16 | 2017-11-17 | 长沙湘资生物科技有限公司 | Utilize the method for Pueraria lobota slag for cultivating Phellinus |
| CN107567956A (en) * | 2017-08-16 | 2018-01-12 | 吉首大学 | Utilize the method for folium cortex eucommiae cultivation handle mushroom |
| CN108293587A (en) * | 2017-08-16 | 2018-07-20 | 江西农业大学 | Utilize the method for Pueraria lobota slag for cultivating elm mushroom mushroom |
| CN109661974A (en) * | 2019-02-20 | 2019-04-23 | 文山学院 | A method of white ginseng bacterium is cultivated using Radix Notoginseng undesirable root |
| CN111937672A (en) * | 2020-07-31 | 2020-11-17 | 湖南科技学院 | Agaric cultivation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766094A (en) * | 2010-01-26 | 2010-07-07 | 黑龙江省珍宝岛制药有限公司 | Black fungus and cultural method and application thereof |
| CN102113462A (en) * | 2011-03-09 | 2011-07-06 | 国家林业局泡桐研究开发中心 | Functional type gingko champignon cultivated by using medicinal plant residues and production method thereof |
| CN102177811A (en) * | 2011-01-18 | 2011-09-14 | 国家林业局泡桐研究开发中心 | Functional eucommia lentinus edodes cultivated by using residua of medicinal plants and production method thereof |
| CN102190512A (en) * | 2010-03-10 | 2011-09-21 | 四川省中医药科学院 | Fungus plant culture medium containing non-medicinal parts of Chinese medicinal materials and application thereof to culture of auricularia polytricha (mont) sacl |
-
2012
- 2012-04-23 CN CN 201210118510 patent/CN102630492B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766094A (en) * | 2010-01-26 | 2010-07-07 | 黑龙江省珍宝岛制药有限公司 | Black fungus and cultural method and application thereof |
| CN102190512A (en) * | 2010-03-10 | 2011-09-21 | 四川省中医药科学院 | Fungus plant culture medium containing non-medicinal parts of Chinese medicinal materials and application thereof to culture of auricularia polytricha (mont) sacl |
| CN102177811A (en) * | 2011-01-18 | 2011-09-14 | 国家林业局泡桐研究开发中心 | Functional eucommia lentinus edodes cultivated by using residua of medicinal plants and production method thereof |
| CN102113462A (en) * | 2011-03-09 | 2011-07-06 | 国家林业局泡桐研究开发中心 | Functional type gingko champignon cultivated by using medicinal plant residues and production method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 孙笑槐: "银杏叶中有效成分的研究进展", 《中国科技信息》 * |
| 陈丽华等: "8味中药对黑木耳发酵的影响", 《食品与生物技术学报》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104115671A (en) * | 2014-07-18 | 2014-10-29 | 国家林业局泡桐研究开发中心 | Dogwood lentinula edodes cultivating method |
| CN104115671B (en) * | 2014-07-18 | 2016-03-30 | 国家林业局泡桐研究开发中心 | A kind of production method of cultivating dogwood mushroom |
| CN104311284A (en) * | 2014-09-26 | 2015-01-28 | 侯中仁 | Preparation method of black fungus culture medium |
| CN105123401A (en) * | 2015-08-25 | 2015-12-09 | 全椒林农生态农业科技有限公司 | Method for interplanting apocarya with salvia miltiorrhiza |
| CN105766377A (en) * | 2016-04-19 | 2016-07-20 | 牡丹江师范学院 | Culture method capable of increasing the flavonoid content and varieties of auricularia auricula |
| CN106365831A (en) * | 2016-08-30 | 2017-02-01 | 杭州云开生态农业开发有限公司 | Auricularia auricula culture medium and preparation method thereof |
| CN106818116A (en) * | 2016-12-20 | 2017-06-13 | 长兴众化生物科技有限公司 | A kind of method that FLOS CHRYSANTHEMI ALBA from Haizhou of China is planted in Zhejiang area salt-soda soil |
| CN107176872A (en) * | 2017-02-10 | 2017-09-19 | 邢建国 | A kind of production method of feature agaric |
| CN107347452A (en) * | 2017-08-16 | 2017-11-17 | 长沙湘资生物科技有限公司 | Utilize the method for Pueraria lobota slag for cultivating Phellinus |
| CN107567956A (en) * | 2017-08-16 | 2018-01-12 | 吉首大学 | Utilize the method for folium cortex eucommiae cultivation handle mushroom |
| CN108293587A (en) * | 2017-08-16 | 2018-07-20 | 江西农业大学 | Utilize the method for Pueraria lobota slag for cultivating elm mushroom mushroom |
| CN109661974A (en) * | 2019-02-20 | 2019-04-23 | 文山学院 | A method of white ginseng bacterium is cultivated using Radix Notoginseng undesirable root |
| CN111937672A (en) * | 2020-07-31 | 2020-11-17 | 湖南科技学院 | Agaric cultivation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102630492B (en) | 2013-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102630492B (en) | Production method for cultivating gingko agaric by utilizing medicinal plant residues | |
| CN102113462B (en) | Functional type gingko champignon cultivated by using medicinal plant residues and production method thereof | |
| CN103553839B (en) | Special compound fertilizer for corn and preparation method thereof | |
| CN102523934B (en) | Production method for cultivating functional eucommia wood ear | |
| CN102177811B (en) | Functional eucommia lentinus edodes cultivated by using residua of medicinal plants and production method thereof | |
| CN103130532A (en) | Preparation method of special base fertilizer for plant honeysuckle | |
| CN106348852A (en) | Planting fertilizer and preparation method for red dragon fruit | |
| CN103420731A (en) | Compatibility of ganoderma lucidum cultivation material and preparation method of ganoderma lucidum cultivation material | |
| CN108055958A (en) | A kind of method that peppermint is interplanted in citrus orchard | |
| CN107652048A (en) | A kind of cultivation matrix of mushroom enhancing immunization and preparation method thereof | |
| CN104285772B (en) | Cultivating substrate and preparation method thereof and ginseng planting method | |
| CN103524192B (en) | Formula and preparing method of base fertilizer and leaf fertilizer for increasing apple production | |
| CN105418233A (en) | Special organic fertilizer for planting adinandra millettii | |
| CN107417414A (en) | A kind of citrus ecological fertilizer special | |
| CN106588367A (en) | Special fertilizer for promoting growth of grapes | |
| CN107417470A (en) | A kind of soybean ecological fertilizer special | |
| CN102303995A (en) | Organic vegetable insecticide-fertilizer and preparation method thereof | |
| CN108715571A (en) | A kind of corn planting dedicated fertilizer | |
| Bhutia et al. | Wild edible plant used by ethnic peoples on the boundary of subtropical & temperate natural forest, Mamlay watershed, South Sikkim | |
| CN109503228A (en) | A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk | |
| CN109006317A (en) | A kind of cane planting method | |
| CN107512947A (en) | A kind of cotton ecological fertilizer special | |
| CN104115671B (en) | A kind of production method of cultivating dogwood mushroom | |
| KR20060134247A (en) | Feed made of yellow earth and method for producing the same | |
| CN106588489A (en) | Special chemical fertilizer capable of improving grape fruit quality |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130522 Termination date: 20160423 |