CN109503228A - A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk - Google Patents

A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk Download PDF

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CN109503228A
CN109503228A CN201811455612.4A CN201811455612A CN109503228A CN 109503228 A CN109503228 A CN 109503228A CN 201811455612 A CN201811455612 A CN 201811455612A CN 109503228 A CN109503228 A CN 109503228A
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garlic stalk
ludwig
ludwig enterobacteria
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CN109503228B (en
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周方园
张新建
吴晓青
赵晓燕
周红姿
范素素
张广志
谢雪迎
王加宁
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Ecology Institute Shandong Academy Of Sciences
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The present invention relates to a kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk, liquid bacterial manure includes garlic stalk, potassium dihydrogen phosphate, soy meal, corn flour, glucose, yeast powder, water and Ludwig enterobacteria LB fermentation liquid；The Ludwig enterobacteria is Ludwig enterobacteriaEnterobacter ludwigiiB424, the deposit number of the bacterial strain are CGMCC No.14915.The depositary institution of the bacterial strain is China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: China, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Preservation date: on November 16th, 2017.It is produced by the invention that there is promotion plant growth, disease-resistant multifunctional liquid bacterial manure, reduce the use of farmland fertilizer pesticide, improves farmland ecological environment with great economic benefit, social benefit and ecological benefits.

Description

A kind of liquid bacterial manure and its preparation using Ludwig enterobacteria fermented garlic stalk Method
Technical field
The present invention relates to Straw manures production technical fields, are fermented more particularly to a kind of using Ludwig enterobacteria Liquid bacterial manure of garlic stalk and preparation method thereof.
Background technique
China is main producing region and the first large outlet state of world garlic, produces 11000000 tons of garlic or more per year, accounts for world's total output 66% or more of amount, Shandong is the main producing region of China garlic.The global annual garlic stalk generated more than 3,700,000 tons, such as processing are not In time, serious environmental pollution and other ecological problems can be brought.Garlic stalk is full of nutrition, do not recycle cause it is serious The wasting of resources.
Currently, garlic stalk is carried out returning to the field by existing research person, such as Publication No. CN103238445A provides a kind of straw Stalk returning to the field formula selenium-enriched garlic implantation methods, including choose seeds, select plantation time, Cultivate administration；Seed selection: selection garlic Greatly, garlic skin sheath is tight, and the pure white solid water content of garlic meat is high, and the garlic of a garlic 5-6 valve is planted；The selection plantation time: soil Freeze 10 centimetres of beginnings to complete between the Spring Equinox；Implantation methods: garlic stalk is crushed, and is sufficiently fermented using stover ferment agent, According to the application method of organic fertilizer, tiling or ditch spread when use.But garlic stalk returning to the field is difficult to avoid that because of returning to the field bring The problem of crop disease aggravates.
Also after thering is patent to be handled garlic stalk as the culture substrate of edible mushroom, arable farming matrix or directly It is used as feed.For example, Publication No. CN104402616A, CN104402617A, CN104402618A, which disclose one kind, to be made It is to be prepared by the raw material of following parts by weight: garlic stalk powder with the oyster mushroom of garlic stalk, pleurotus cornucopiae, planting almond abalone mushroom matrix Mince 30-60 parts, 30-60 parts of cotton seed hulls, 10-20 parts of wheat bran, 1-3 part of quick lime, and 1-2 part of gypsum, urea 0.5-1 Part, 0.5-1 part of calcium superphosphate.Publication No. CN102584354A is disclosed a kind of prepared using garlic stalk waste and eaten The method of bacterium culture medium matter is put into the garlic stalk through grinding in the steamer of steam distillation device, with contain big allinase Aqueous solution be sprayed on the garlic stalk through grinding, then using steam distillation method extract garlic oil, extract garlic oil Garlic stalk afterwards is used for compounding culture medium of edible fungus matter.Also patent is added to it on a small quantity for garlic stalk as one of component Arable farming matrix is made in his material.Publication No. CN108377882A provides a kind of capsicum soilless culture substrate and its system Preparation Method, the raw material comprising following parts by weight: 25-- 45 parts of peat, 30-50 parts of fermentation of biomass, carbonization rice husk 5-10 Part, 5-10 parts of fishbone dust, 5-10 parts of bentonite, 1-5 part of Celastrus angulatus straw powder, 1-5 part of oyster shell powder.The fermenting organism Matter includes the raw material of following parts by weight: 20-40 parts of sugarcane bagasse, 10-20 parts of rapeseed dregs, 10-20 parts of animal wastes, peanut shell 10-20 parts of powder, 5-10 parts of toosendanin straw powder, 5-10 parts of garlic stalk powder, 5-10 parts of agricultural residues powder, microbial bacteria 1-5 part of agent.Publication No. CN107912266A discloses a kind of seedling medium for enhancing tomato disease resistance, by following raw material Be made: the Tomato Seed dregs of rice, garlic stalk, mushroom waste material, grape skin, sheep dung, sulphur powder, pond sludge, Galangin, in Herba Andrographitis Ester, Rhizoma Arisaematis extract, manganese chloride.Publication No. CN108142229A discloses a kind of nursery base for improving rice nutrition quality Matter is prepared from the following raw materials: dregs of beans, pig manure, citrus peel residue, diatomite, river silt, garlic stalk, fresh pine needle, fresh Herba Andrographitis, corruption POTASSIUM PHYTATE, polyacrylamide.Publication No. CN107176864A disclose it is a kind of first grinding stirring after heat-preservation fermentation biology training Nutriment preparation method, include the following steps: S1, configuration biological culture material: the raw material of material A includes cotton seed hulls, bagasse, wine Slot, rice bran, pine tree sawdust, soybean powder, palm shell, rotted pig manure, sucrose, precipitated calcium carbonate, calcium superphosphate, potassium dihydrogen phosphate, Magnesium sulfate and vitamin B；The raw material of material B include Prunella vulgaris slag, coconut shell powder, walnut shell powder, Chinese chestnut bud husk powder, lotus shell powder, Banana stem leaves, honeysuckle cauline leaf, root of Dahurain angelica stalk, ginkgo leaf, garlic stalk, Cassava stalk bits, rice grass meal, Risleya atropurpurea pool, cus-cus Grass, awns beanstalk, napier grass, Poplar leaves, ganoderma lucidum fruitbody, apple pomace, mushroom bran, the wooden chaff slag and cyanobacteria；S2, the configured biology of S1 is trained Nutriment is placed in grinding agitating device and obtains.Also there is patent using garlic stalk as greenfeed, such as Publication No. CN103844002B discloses a kind of production method of whole strain garlic stalk ensilage.
In the patent using garlic stalk production organic fertilizer, add on a small quantity after mainly crushing garlic stalk as auxiliary material It is added in organic fertilizer.If Publication No. CN107686396A discloses a kind of tea tree special slow release potash fertilizer and preparation method thereof, relate to And fertilizer more particularly to slow-release potassium fertilizer；The slow-release potassium fertilizer by bur clover, garlic stalk, selenium dioxide, pine needle meal, wormcast, Potassium chloride, nano-sized carbon, nanoscale potassium feldspar, nano-kaoline, nanoscale montmorillonite, cellulase, lactic acid bacteria, wine brewing ferment Mother, carboxymethyl cellulose, rosin, soybean lecithin is fermented, be granulated, coating is prepared.Publication No. CN107892623A is public A kind of biological organic fertilizer for improving rice content of mineral substances has been opened, has been prepared from the following raw materials: is sheep dung, pig manure, compound biological bacterium, big Garlic stalk, the Tomato Seed dregs of rice, diatomite, borax, copper acetate, humic acid potassium, additive.Publication No. CN108002934A is disclosed A kind of soil-repairing agent with disinsection efficiency, the raw material including following parts by weight: 40-80 parts of organic fertilizer, crotons powder 10-15 Part, 10-20 parts of durian peel powder, 8-15 parts of black bean powder, 8-15 parts of offal powder, 10-20 parts of garlic stalk, 10-15 parts of honeycomb, great Ye Grow tea 6-8 parts, it is 4-8 parts of fourth pot leaf, 3-7 parts of fructus cannabis, 1-3 parts of zineb, 2-5 parts of Baeckea frutescens L., 2-5 parts of nano aluminium oxide, peppery 1-3 parts of root, 20-50 parts of sugar-cane juice, 2-5 parts of selenium-enriched protein, 2-5 parts of bitooth multi-ant, 50-100 parts of water.Publication No. CN106866194A provides a kind of preparation method of biogas slurry organic fertilizer, comprising: S1. mixed raw material；S2. it ferments for the first time；S3. Second of fermentation；S4. dry powder processed.The mixed raw material includes: according to mass percent, animal wastes 50-80%, stalk (its In include garlic stalk) 5-30%, water hyacinth 5-30%.The method of fermentation for the first time are as follows: methane-generating pit is added in mixed raw material In ferment；The biogas residue and biogas slurry in methane-generating pit are then taken out, and is separated by solid-liquid separation.The method of second of fermentation are as follows: take Liquid product after fermenting for the first time；Garlic stalk is added in the liquid product；Then zymophyte is added to ferment；Most Afterwards to take liquid phase part therein stand-by after fermentation.Publication No. CN106748184A discloses a kind of tea of pre- insect-pest Tree planting organic fertilizer and preparation method thereof, is made of raw material from the following weight: zeolite, 2mol/L Na2HPO4Solution, chlorination Calcium, the leaf of bamboo, sugarcane top, maize leaves, ginger slag, garlic stalk, EM bacteria agent, the perillaseed dregs of rice, kuh-seng, agar powder, chitin, corn Face, peat soil and suitable water.Publication No. CN106396921A discloses a kind of insect prevention disease-resistant organic fertilizer and preparation method thereof； The organic fertilizer is formulated according to parts by weight by following raw materials according: 20-40 parts of tobacco leaf stalk, 10-15 parts of garlic stalk, onion straw 10-15 parts of stalk, 20-40 parts of fowl and animal excrement, 20-51 parts of straw, 0.15-0.25 parts of microbial inoculum, 10-15 parts of kuh-seng.Publication number A kind of strawberry continuous cropping soil ecology organic fertilizer and preparation method thereof is disclosed for CN105837292A, which is by that will mix Conjunction has ground phosphate rock, potassium silicate powder, Radix Isatidis stalk powder, garlic stalk powder, methane-oxidizing bacteria, nitrogen-fixing bacteria, phosphobacteria and potassium Uniformly addition bacillus subtilis, bacillus amyloliquefaciens and streptomycete mixing again after the feces of livestock and poultry of bacterium is banked up fermentation It forms.Publication No. CN105198607A discloses a kind of preparation method of more nutrition sterilization and disinsection Blending Fertilizer auxiliary materials, including with Lower step: quassia, garlic stalk, chinaberry root, turmeric, Fructus Forsythiae are dried for the first time, crushed, sieving obtains material A；By material A It puts into prefabricated extracting solution, is ultrasonically treated after mixing evenly, then filtered, be concentrated to get feed liquid B；By feed liquid B, gelatin, Chitosan, attapulgite, dicyclohexyl phthalate, low molecular poly, ethyl alcohol are uniformly mixed, and are dried to obtain medium-height grass Medicine activity sterilizing insecticide；After mixing by Chinese herbal medicine activity sterilizing insecticide and decomposed manure, it is granulated and obtains more nutrition Sterilization and disinsection Blending Fertilizer auxiliary material.Publication No. CN104761406B discloses a kind of shallot special fertilizer material and preparation method thereof, institute It states organic fertilizer to be made of raw material from the following weight: fowl and animal excrement 40-65, peanut shell 10-30, wheat bran 5-20, fresh garlic straw Stalk 5-20, fresh garlic garlic 3-10, discarded Chinese medicine slag 5-20, traditional Chinese medicinal components 3-10.Publication No. CN102134169A is disclosed A kind of manufacturing method of biological organic fertilizer, the weight ratio of ingredient are as follows: cow dung content is 20% -50%, straw 20% - 50%, garlic stalk 0-50%, land plaster 1%, pulverized limestone 1%-2%.Publication No. CN108002955A discloses one kind Can the salt affected soil improving fertilizer and preparation method thereof of pre- preventing disease and pest be formulated according to parts by weight by following raw materials according: tobacco leaf 10-20 parts, 10-20 parts of garlic stalk, 20-50 parts of excrement, straw (including garlic stalk) 10-30 parts, seawood meal 10-15 Part, 15-30 parts of zeolite, 15-20 parts of kuh-seng, 10-15 parts of potassium dihydrogen phosphate, 5-10 parts of magnesium sulfate, 0.5-1 parts of saccharomycete, gemma 0.3-1 parts of bacterium, 50-100 parts of domestic sludge.Publication No. CN107892623A, which is disclosed, a kind of improves rice content of mineral substances Biological organic fertilizer is prepared from the following raw materials: sheep dung, pig manure, compound biological bacterium, garlic stalk, the Tomato Seed dregs of rice, diatomite, borax, Copper acetate, humic acid potassium, additive.Publication No. CN107698342A, which is disclosed, a kind of improves rose bioactive ingredients The preparation method of biogas slurry nutritional agents is biogas slurry, grape skin, scarlet specifically includes the following steps: (1) weighs raw material by weight Potato cane leaves, fresh mulberries leaf, garlic stalk, Morinda officinalis, glutinous rehmannia, Pediococcus pentosaceus, water；(2) fermentation slurry is obtained；(3) fermentation liquid is obtained； (4) fermentation liquid is subjected to magnetization treatment, obtains biogas slurry nutritional agents.It is true that Publication No. CN106977249A discloses a kind of selenium-rich grape Bacterial manure must be fermented by garlic stalk, chicken manure, astragalus root dregs, furfural dregs, soft pin, eggshell, brown sugar progress microbial fermentation Bio-fertilizer, and fermenting organism fertilizer is obtained after fungi plantation conversion.
Through retrieving, not yet finds that with garlic stalk be main fermentation substrate, utilizes the Ludwig with antifungal activity Enterobacteria develops relevant report as the bacterial manure of fermenting microbe.
Summary of the invention
In order to solve the inefficient utilization of garlic stalk and the pollution problem to environment, the present invention provides one kind with garlic Stalk is main fermentation substrate, uses with antifungal activity, promotes the Ludwig enterobacteria of plant growth as zymophyte The liquid bacterial manure and preparation method thereof of strain.The present invention uses Ludwig enterobacteriaEnterobacter ludwigiiB424, The deposit number of the bacterial strain is CGMCC No.14915.The depositary institution of the bacterial strain is Chinese microorganism strain preservation conservator Meeting common micro-organisms center, address: China, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Preservation date: November 16 in 2017 Day.
Ludwig enterobacteria in the present inventionEnterobacter ludwigiiB424 is isolated from garlic field rhizosphere soil And garlic maggot body surface and enteron aisle, the Ludwig enterobacteriaEnterobacterludwigiiB424,16S rDNA sequence Column overall length is 1444bp,
Technical scheme is as follows:
A kind of liquid bacterial manure using Ludwig enterobacteria fermented garlic stalk, the raw material including following parts by weight:
Garlic stalk, potassium dihydrogen phosphate, soy meal, corn flour, glucose, yeast powder, water and Ludwig enterobacteria LB fermentation Liquid.
The preparation method of the Ludwig enterobacteria LB fermentation liquid uses LB culture medium culture Ludwig enterobacteriaEnterobacterludwigiiB424 works as OD600Up to Ludwig enterobacteria LB fermentation liquid when for 0.6-0.8.
Further, described to use Ludwig enterobacteria fermented garlic stalk when garlic stalk is fresh garlic stalk Liquid bacterial manure, the raw material including following parts by weight:
30-40 parts of fresh garlic stalk, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast 0.6 part of powder, 50 parts, 230 parts of water of Ludwig enterobacteria LB fermentation liquid.
When garlic stalk is garlic stalk dry powder, the liquid bacterium with Ludwig enterobacteria fermented garlic stalk Fertilizer, the raw material including following parts by weight:
15-20 parts of garlic stalk dry powder, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast 0.6 portion of powder, 50 parts, 250 parts of water of Ludwig enterobacteria LB fermentation liquid.
In the present invention, garlic stalk is the part that garlic and garlic stems are removed in the whole strain of garlic.
The preparation method of the above-mentioned liquid bacterial manure with Ludwig enterobacteria fermented garlic stalk, the specific steps are as follows:
(1) LB culture medium culture Ludwig enterobacteria, OD are used600To terminate to cultivate when 0.6-0.8, Ludwig intestines are obtained Bacillus LB fermentation liquid；
(2) grinding of fresh garlic stalk is slurried or directly using garlic stalk is dry plus the parts by weight water such as powder, then with soy meal, Corn flour, glucose, yeast powder and remaining water after mixing, obtain mixture；
(3) mixture obtained in (2) is added in Ludwig enterobacteria LB fermentation liquid, 30 DEG C, 180 rpm/min continuation Fermentation 15 days；
(4) potassium dihydrogen phosphate is added in the tunning in (3).
Beneficial effects of the present invention are as follows:
Ludwig enterobacteria in the present inventionEnterobacterludwigiiB424 is isolated from garlic field rhizosphere soil and garlic Maggot body surface and enteron aisle；It is living that the bacterium can adapt to garlic plant strain growth environment, the antibiotics in degradation garlic plant, generation antibacterial Property compound, plant auxin secretion.The present invention uses garlic maggot concomitance bacterium fermented garlic stalk, in the process in degrading straw Inhibit the allelochemical of other plant growths, generates the compound that there is antibacterial activity to various plants pathogen and promotion plant The reactive compound of growth is used using this fermentation material as bacterial manure, is solved the problems, such as the resource utilization of garlic stalk, is relieved Garlic stalk to the pollution of farm environment, alleviate the carrying pressure of farmland ecosystem, produce high value added product, reduce The usage amount of farmland agriculture chemical；Meanwhile it is produced by the invention with promotion plant growth, disease-resistant multifunctional liquid bacterium Fertilizer reduces the use of farmland fertilizer pesticide, improves farmland ecological environment with great economic benefit, social benefit and life State benefit.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and It is apparent.But examples are merely exemplary, and it is not intended to limit the scope of the present invention in any way.Those skilled in the art answer It should be appreciated that without departing from the spirit and scope of the invention can details to technical solution of the present invention and form repair Change or replace, but these modifications and replacement are fallen within the protection scope of the present invention.
A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk of embodiment 1
Liquid bacterial manure includes the raw material of following parts by weight:
30 parts of fresh garlic stalk, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast powder 0.6 part, 50 parts, 230 parts of water of Ludwig enterobacteria LB fermentation liquid；The preparation side of the Ludwig enterobacteria LB fermentation liquid Method uses LB culture medium culture Ludwig enterobacteriaEnterobacter ludwigii B424 works as OD600When being 0.7 to obtain the final product Ludwig enterobacteria LB fermentation liquid；
Preparation method is as follows:
(1) LB culture medium culture Ludwig enterobacteria, OD are used600Terminate to cultivate when being 0.7, obtains Ludwig enterobacteria LB fermentation liquid；
(2) grinding of fresh garlic stalk is slurried or directly using garlic stalk is dry plus the parts by weight water such as powder, then with soy meal, Corn flour, glucose, yeast powder and remaining water after mixing, obtain mixture；
(3) mixture obtained in (2) is added in Ludwig enterobacteria LB fermentation liquid, 30 DEG C, 180 rpm/min continuation Fermentation 15 days；
(4) potassium dihydrogen phosphate is added in the tunning in (3).
The Ludwig enterobacteriaEnterobacter ludwigiiThe deposit number of B424 is CGMCC No.14915, depositary institution are China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China, Beijing The institute 3 of city, North Star West Road, Chaoyang District 1；The deposit date is on November 16th, 2017.
20 kilograms of liquid bacterial manure that the present embodiment obtains are administered to 3-5 leaf phase tomato seedling with watering, 1 mu of administration area Ground (667 square metres), after application, tomato is because of root disease incidence decline 23.2%, volume increase 5.7%.
A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk of embodiment 2
Liquid bacterial manure includes the raw material of following parts by weight:
35 parts of fresh garlic stalk, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast powder 0.6 part, 50 parts, 230 parts of water of Ludwig enterobacteria LB fermentation liquid；
The preparation method of the Ludwig enterobacteria LB fermentation liquid uses LB culture medium culture Ludwig enterobacteriaEnterobacter ludwigii B424 works as OD600Up to Ludwig enterobacteria LB fermentation liquid when being 0.6；
Preparation method is as follows:
(1) LB culture medium culture Ludwig enterobacteria, OD are used600Terminate to cultivate when being 0.6, obtains Ludwig enterobacteria LB fermentation liquid；
(2) grinding of fresh garlic stalk is slurried or directly using garlic stalk is dry plus the parts by weight water such as powder, then with soy meal, Corn flour, glucose, yeast powder and remaining water after mixing, obtain mixture；
(3) mixture obtained in (2) is added in Ludwig enterobacteria LB fermentation liquid, 30 DEG C, 180 rpm/min continuation Fermentation 15 days；
(4) potassium dihydrogen phosphate is added in the tunning in (3).
The Ludwig enterobacteriaEnterobacter ludwigiiThe deposit number of B424 is CGMCC No.14915, depositary institution are China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China, Beijing The institute 3 of city, North Star West Road, Chaoyang District 1；The deposit date is on November 16th, 2017.
20 kilograms of liquid bacterial manure that the present embodiment obtains are administered to 3-5 leaf phase tomato seedling with watering, 1 mu of administration area Ground (667 square metres), after application, tomato is because of root disease incidence decline 21.1%, volume increase 6.2%.
A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk of embodiment 3
Liquid bacterial manure includes the raw material of following parts by weight:
40 parts of fresh garlic stalk, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast powder 0.6 part, 50 parts, 230 parts of water of Ludwig enterobacteria LB fermentation liquid；
The preparation method of the Ludwig enterobacteria LB fermentation liquid uses LB culture medium culture Ludwig enterobacteriaEnterobacter ludwigii B424 works as OD600Up to Ludwig enterobacteria LB fermentation liquid when being 0.8；
Preparation method is as follows:
(1) LB culture medium culture Ludwig enterobacteria, OD are used600Terminate to cultivate when being 0.8, obtains Ludwig enterobacteria LB fermentation liquid；
(2) grinding of fresh garlic stalk is slurried or directly using garlic stalk is dry plus the parts by weight water such as powder, then with soy meal, Corn flour, glucose, yeast powder and remaining water after mixing, obtain mixture；
(3) mixture obtained in (2) is added in Ludwig enterobacteria LB fermentation liquid, 30 DEG C, 180 rpm/min continuation Fermentation 15 days；
(4) potassium dihydrogen phosphate is added in the tunning in (3).
The Ludwig enterobacteriaEnterobacter ludwigiiThe deposit number of B424 is CGMCC No.14915, depositary institution are China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China, Beijing The institute 3 of city, North Star West Road, Chaoyang District 1；The deposit date is on November 16th, 2017.
20 kilograms of liquid bacterial manure that the present embodiment obtains are administered to 3-5 leaf phase tomato seedling with watering, 1 mu of administration area Ground (667 square metres), after application, tomato is because of root disease incidence decline 22.4%, volume increase 5.8%.
A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk of embodiment 4
Liquid bacterial manure includes the raw material of following parts by weight:
15 parts of garlic stalk dry powder, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast powder 0.6 part, 50 parts, 250 parts of water of Ludwig enterobacteria LB fermentation liquid；
The preparation method of the Ludwig enterobacteria LB fermentation liquid uses LB culture medium culture Ludwig enterobacteriaEnterobacter ludwigii B424 works as OD600Up to Ludwig enterobacteria LB fermentation liquid when being 0.6；
Preparation method is as follows:
(1) LB culture medium culture Ludwig enterobacteria, OD are used600Terminate to cultivate when being 0.6, obtains Ludwig enterobacteria LB fermentation liquid；
(2) grinding of fresh garlic stalk is slurried or directly using garlic stalk is dry plus the parts by weight water such as powder, then with soy meal, Corn flour, glucose, yeast powder and remaining water after mixing, obtain mixture；
(3) mixture obtained in (2) is added in Ludwig enterobacteria LB fermentation liquid, 30 DEG C, 180 rpm/min continuation Fermentation 15 days；
(4) potassium dihydrogen phosphate is added in the tunning in (3).
The Ludwig enterobacteriaEnterobacter ludwigiiThe deposit number of B424 is CGMCC No.14915, depositary institution are China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China, Beijing The institute 3 of city, North Star West Road, Chaoyang District 1；The deposit date is on November 16th, 2017.
20 kilograms of liquid bacterial manure that the present embodiment obtains are administered to 3-5 leaf phase corn seedling with watering, 1 mu of administration area Ground (667 square metres), after application, corn is because of root disease incidence decline 10.3%, volume increase 8.2%.A kind of utilization of embodiment 5 The liquid bacterial manure and preparation method thereof of Ludwig enterobacteria fermented garlic stalk
Liquid bacterial manure includes the raw material of following parts by weight:
20 parts of garlic stalk dry powder, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast powder 0.6 part, 50 parts, 250 parts of water of Ludwig enterobacteria LB fermentation liquid；
The preparation method of the Ludwig enterobacteria LB fermentation liquid uses LB culture medium culture Ludwig enterobacteriaEnterobacter ludwigiiB424 works as OD600Up to Ludwig enterobacteria LB fermentation liquid when being 0.7；
Preparation method is as follows:
(1) LB culture medium culture Ludwig enterobacteria, OD are used600Terminate to cultivate when being 0.7, obtains Ludwig enterobacteria LB fermentation liquid；
(2) grinding of fresh garlic stalk is slurried or directly using garlic stalk is dry plus the parts by weight water such as powder, then with soy meal, Corn flour, glucose, yeast powder and remaining water after mixing, obtain mixture；
(3) mixture obtained in (2) is added in Ludwig enterobacteria LB fermentation liquid, 30 DEG C, 180 rpm/min continuation Fermentation 15 days；
(4) potassium dihydrogen phosphate is added in the tunning in (3).
The Ludwig enterobacteriaEnterobacter ludwigiiThe deposit number of B424 is CGMCC No.14915, depositary institution are China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China, Beijing The institute 3 of city, North Star West Road, Chaoyang District 1；The deposit date is on November 16th, 2017.
The Ludwig enterobacteriaEnterobacter ludwigii The deposit number of B424, the bacterial strain is CGMCC No. 14915, has been saved in China Committee for Culture Collection of Microorganisms's common micro-organisms center.
20 kilograms of liquid bacterial manure that the present embodiment obtains are administered to tillering stage wheat paddock, 1 mu of ground of administration area with watering (667 square metres), after application, wheat later period root disease incidence decline 13.7%, volume increase 6.2%.

Claims (8)

1. a kind of liquid bacterial manure using Ludwig enterobacteria fermented garlic stalk, which is characterized in that liquid bacterial manure include with Lower raw material:
Garlic stalk, potassium dihydrogen phosphate, soy meal, corn flour, glucose, yeast powder, water and Ludwig enterobacteria LB fermentation Liquid.
2. liquid bacterial manure according to claim 1, which is characterized in that the Ludwig enterobacteria is Ludwig intestines bar Bacterium Enterobacter ludwigii B424, the deposit number of the bacterial strain are CGMCC No.14915,
The depositary institution of the bacterial strain is China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: China, north The institute 3 of the Chaoyang District Jing Shi North Star West Road 1；Preservation date: on November 16th, 2017.
3. liquid bacterial manure according to claim 2, which is characterized in that the preparation of the Ludwig enterobacteria LB fermentation liquid Method works as OD using LB culture medium culture Ludwig enterobacteria Enterobacterludwigii B424600For 0.6-0.8 When up to Ludwig enterobacteria LB fermentation liquid.
4. liquid bacterial manure according to claim 3, which is characterized in that the preparation of the Ludwig enterobacteria LB fermentation liquid Method works as OD using LB culture medium culture Ludwig enterobacteria Enterobacterludwigii B424600When being 0.7 Obtain Ludwig enterobacteria LB fermentation liquid.
5. liquid bacterial manure according to claim 3, which is characterized in that described when garlic stalk is fresh garlic stalk With the liquid bacterial manure of Ludwig enterobacteria fermented garlic stalk, the raw material including following parts by weight:
30-40 parts of fresh garlic stalk, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast 0.6 part of powder, 50 parts, 230 parts of water of Ludwig enterobacteria LB fermentation liquid.
6. liquid bacterial manure according to claim 3, which is characterized in that described when garlic stalk is garlic stalk dry powder With the liquid bacterial manure of Ludwig enterobacteria fermented garlic stalk, the raw material including following parts by weight:
15-20 parts of garlic stalk dry powder, 15 parts of potassium dihydrogen phosphate, 10 parts of soy meal, 6 parts of corn flour, 1.5 parts of glucose, yeast 0.6 portion of powder, 50 parts, 250 parts of water of Ludwig enterobacteria LB fermentation liquid.
7. liquid bacterial manure according to claim 1, which is characterized in that the garlic stalk be the whole strain of garlic remove garlic and The part of garlic stems.
8. the method for preparing the liquid bacterial manure as described in claim any one of 1-7, the specific steps are as follows:
(1) LB culture medium culture Ludwig enterobacteria, OD are used600To terminate to cultivate when 0.6-0.8, Ludwig intestines are obtained Bacillus LB fermentation liquid；
(2) grinding of fresh garlic stalk is slurried or directly using garlic stalk is dry plus the parts by weight water such as powder, then with soy meal, Corn flour, glucose, yeast powder and remaining water after mixing, obtain mixture；
(3) mixture obtained in (2) is added in Ludwig enterobacteria LB fermentation liquid, 30 DEG C, 180 rpm/min continuation Fermentation 15 days；
(4) potassium dihydrogen phosphate is added in the tunning in (3).
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