CN109503228B - Liquid bacterial fertilizer for fermenting garlic straw by virtue of enterobacter ludwigii and preparation method of liquid bacterial fertilizer - Google Patents

Liquid bacterial fertilizer for fermenting garlic straw by virtue of enterobacter ludwigii and preparation method of liquid bacterial fertilizer Download PDF

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CN109503228B
CN109503228B CN201811455612.4A CN201811455612A CN109503228B CN 109503228 B CN109503228 B CN 109503228B CN 201811455612 A CN201811455612 A CN 201811455612A CN 109503228 B CN109503228 B CN 109503228B
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garlic
enterobacter ludwigii
liquid bacterial
enterobacter
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CN109503228A (en
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周方园
张新建
吴晓青
赵晓燕
周红姿
范素素
张广志
谢雪迎
王加宁
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Ecology Institute Shandong Academy Of Sciences
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention relates to a liquid bacterial fertilizer for fermenting garlic straws by using enterobacter ludwigii and a preparation method thereof, wherein the liquid bacterial fertilizer comprises garlic straws, monopotassium phosphate, soybean meal, corn flour, glucose, yeast powder, water and an LB fermentation liquor of the enterobacter ludwigii; the Enterobacter ludwigii is Enterobacter ludwigiiEnterobacter ludwigiiB424, the preservation number of the strain is CGMCC No. 14915. The preservation unit of the strain is China general microbiological culture Collection center, and the address is as follows: west road No.1, north chen, north china, beijing, chaoyang district; the preservation date is as follows: 11 and 16 months in 2017. The multifunctional liquid bacterial fertilizer produced by the invention has the advantages of promoting the growth of crops and resisting diseases, reducing the use of chemical fertilizers and pesticides in farmlands, improving the ecological environment of farmlands, and having great economic, social and ecological benefits.

Description

Liquid bacterial fertilizer for fermenting garlic straw by virtue of enterobacter ludwigii and preparation method of liquid bacterial fertilizer
Technical Field
The invention relates to the technical field of straw organic fertilizer production, in particular to a liquid bacterial fertilizer for fermenting garlic straws by using enterobacter ludwigii and a preparation method thereof.
Background
China is the main production area and the first major export country of garlic in the world, more than 1100 million tons of garlic are produced annually, which accounts for more than 66% of the total production in the world, and Shandong is the main production area of garlic in China. More than 370 million tons of garlic straws are produced in the world every year, and if the garlic straws are not processed in time, serious environmental pollution and other ecological problems can be caused. The garlic straw is rich in nutrition and cannot be recycled, so that serious resource waste is caused.
At present, researchers return garlic straws to fields, for example, the publication number is CN103238445A, provides a straw returning type selenium-enriched garlic planting method, which comprises the steps of seed selection, planting time selection, planting management and the like; seed selection: selecting garlic bulbs and garlic skins to protect the garlic skins tightly, the garlic meats are white, solid and high in water content, and the garlic with 5-6 garlic cloves at one head is used as seeds; selecting planting time: the soil is thawed from 10 cm to the spring festival; the planting method comprises the following steps: the garlic straws are crushed and fully fermented by using a straw fermenting agent, and when the organic fertilizer is used, the garlic straws are spread flatly or applied in a ditch according to the use method of the organic fertilizer. But the problem of crop disease aggravation caused by returning the garlic straws to the field is difficult to avoid.
There are also patents that the garlic straw is used as culture medium of edible fungi, crop cultivation medium or directly used as feed after being treated. For example, publication nos. CN104402616A, CN104402617A, and CN104402618A disclose a cultivation medium for oyster mushroom, pleurotus cornucopiae, and pleurotus eryngii using garlic straw, which is prepared from the following raw materials in parts by weight: 30-60 parts of garlic straw crushed material, 30-60 parts of cottonseed hull, 10-20 parts of wheat bran, 1-3 parts of quicklime, 1-2 parts of gypsum, 0.5-1 part of urea and 0.5-1 part of calcium superphosphate. Publication No. CN102584354A discloses a method for preparing edible fungus culture medium from garlic straw waste, which comprises placing ground garlic straw into a steamer of a steam distillation device, spraying aqueous solution containing alliinase on the ground garlic straw, extracting garlic oil by steam distillation, and using the garlic straw after extracting garlic oil to prepare the edible fungus culture medium. There are also patents in which garlic straw is added in small amounts as one of the components to other materials to make crop cultivation substrates. Publication No. CN108377882A provides a pepper soilless culture substrate and a preparation method thereof, which comprises the following raw materials in parts by weight: 25-45 parts of peat, 30-50 parts of fermented biomass, 5-10 parts of carbonized rice hulls, 5-10 parts of fishbone meal, 5-10 parts of bentonite, 1-5 parts of celastrus angulatus straw powder and 1-5 parts of oyster shell powder. The fermented biomass comprises the following raw materials in parts by weight: 20-40 parts of bagasse, 10-20 parts of rapeseed dregs, 10-20 parts of animal wastes, 10-20 parts of peanut shell powder, 5-10 parts of chinaberry straw powder, 5-10 parts of garlic straw powder, 5-10 parts of crop straw powder and 1-5 parts of microbial agent. Publication No. CN107912266A discloses a seedling raising substrate for enhancing disease resistance of tomatoes, which is prepared from the following raw materials: tomato seed meal, garlic straws, mushroom waste, grape skin residues, sheep manure, sulfur powder, pond sludge, galangin, andrographolide, rhizoma arisaematis extract and manganese chloride. Publication No. CN108142229A discloses a seedling raising substrate for improving the nutritional quality of rice, which is prepared from the following raw materials: bean pulp, pig manure, citrus peel residues, diatomite, river mud, garlic straws, fresh pine needles, fresh andrographis paniculata, potassium humate and polyacrylamide. Publication No. CN107176864A discloses a preparation method of a biological culture material by grinding, stirring and then fermenting under heat preservation, which comprises the following steps: s1, preparing a biological culture material: the raw materials of the material A comprise cottonseed hulls, bagasse, wine lees, rice bran, pine sawdust, soybean meal, palm hulls, decomposed pig manure, cane sugar, light calcium carbonate, calcium superphosphate, monopotassium phosphate, magnesium sulfate and vitamin B; the raw materials of the material B comprise selfheal residues, coconut shell powder, walnut shell powder, chestnut shell powder, lotus shell powder, banana stems and leaves, honeysuckle stems and leaves, angelica dahurica straws, ginkgo leaves, garlic straws, cassava stalk chips, rice grass powder, lacca, vetiver, dicranopteris pedata, elephant grass, poplar leaves, lucid ganoderma sporocarp, apple pomace, mushroom bran, wood bran residues and blue algae; s2, placing the prepared biological culture material of S1 in a grinding and stirring device to obtain the biological culture material. There are also patents which use garlic straw as silage, for example publication No. CN103844002B discloses a method for preparing whole plant garlic straw silage.
In the patent of using garlic straws to prepare the organic fertilizer, the garlic straws are mainly crushed and then used as auxiliary materials to be added into the organic fertilizer in a small amount. For example, the publication No. CN107686396A discloses a special slow-release potash fertilizer for tea trees and a preparation method thereof, relating to fertilizers, in particular to fertilizersIt relates to slow release potash fertilizer; the slow-release potassium fertilizer is prepared from medicago falcata, garlic straws, selenium dioxide, pine needle powder, wormcast, potassium chloride, nano-carbon, nano-scale potassium feldspar, nano-scale kaolin, nano-scale montmorillonite, cellulase, lactic acid bacteria, saccharomyces cerevisiae, carboxymethyl cellulose, rosin and soybean phospholipid through fermentation, granulation and coating. Publication No. CN107892623A discloses a bio-organic fertilizer for increasing the mineral content of rice, which is prepared from the following raw materials: sheep manure, pig manure, composite biological bacteria, garlic straw, tomato seed meal, diatomite, borax, copper acetate, potassium humate and an additive. Publication No. CN108002934A discloses a soil remediation agent with insecticidal effect, which comprises the following raw materials in parts by weight: 40-80 parts of organic fertilizer, 10-15 parts of croton powder, 10-20 parts of durian peel powder, 8-15 parts of black bean powder, 8-15 parts of tobacco stalk powder, 10-20 parts of garlic straw, 10-15 parts of honeycomb, 6-8 parts of big-leaf tea, 4-8 parts of leaf of a clove pot, 3-7 parts of fructus cannabis, 1-3 parts of zineb, 2-5 parts of baeckea frutescens, 2-5 parts of nano aluminum oxide, 1-3 parts of horseradish, 20-50 parts of sugarcane juice, 2-5 parts of selenium-enriched protein, 2-5 parts of polyrhachis dives, and 50-100 parts of water. Publication No. CN106866194A provides a preparation method of a biogas slurry organic fertilizer, which comprises the following steps: s1, mixing raw materials; s2, fermenting for the first time; s3, fermenting for the second time; and S4, drying to prepare powder. The mixed raw materials comprise: according to the mass percentage, the animal manure is 50-80%, the straws (including garlic straws) are 5-30%, and the water hyacinth is 5-30%. The method for the first fermentation comprises the following steps: adding the mixed raw materials into a methane tank for fermentation; then taking out biogas residues and biogas slurry in the biogas digester, and carrying out solid-liquid separation. The method for the second fermentation comprises the following steps: taking a liquid phase product after the first fermentation; adding garlic straw to the liquid phase product; then adding zymophyte for fermentation; and finally, taking the liquid phase part for later use after the fermentation is finished. Publication No. CN106748184A discloses a tea tree planting organic fertilizer for preventing insect pests and a preparation method thereof, wherein the tea tree planting organic fertilizer is prepared from the following raw materials in parts by weight: zeolite, 2mol/L Na2HPO4The fertilizer comprises a solution, calcium chloride, bamboo leaves, sugarcane leaves, corn leaves, ginger residues, garlic straws, EM microbial inoculum, perilla seed meal, radix sophorae flavescentis, agar powder, chitin, corn flour, peat soil and a proper amount of water. Publication No. CN106396921A discloses an insect-proof disease-resistant organic fertilizer and a preparation method thereof; the organic fertilizer is prepared from the following raw materials in parts by weight: 20-40 parts of tobacco leaf straw, 10-15 parts of garlic straw, 10-15 parts of onion straw, 20-40 parts of livestock manure, 20-51 parts of plant straw, 0.15-0.25 part of microbial inoculum and 10-15 parts of radix sophorae flavescentis. Publication No. CN105837292A discloses a strawberry continuous cropping soil ecological organic fertilizer and a preparation method thereof, the organic fertilizer is prepared by stacking and fermenting livestock and poultry manure mixed with phosphate rock powder, potassium silicate powder, isatis root straw powder, garlic straw powder, methane-oxidizing bacteria, nitrogen-fixing bacteria, phosphorus bacteria and potassium bacteria, and then uniformly adding bacillus subtilis, bacillus amyloliquefaciens and streptomyces and mixing. The publication No. CN105198607A discloses a preparation method of a multi-nutrient sterilizing and insecticidal bulk blending fertilizer auxiliary material, which comprises the following steps: drying ramulus Et folium Picrasmae, Bulbus Allii straw, Melia azedarach root, Curcuma rhizome, and fructus forsythiae for the first time, pulverizing, and sieving to obtain material A; putting the material A into the prefabricated extracting solution, uniformly stirring, carrying out ultrasonic treatment, filtering, and concentrating to obtain a material liquid B; uniformly mixing the feed liquid B, gelatin, chitosan, attapulgite, dicyclohexyl phthalate, low-molecular-weight polyethylene glycol and ethanol, and drying to obtain the Chinese herbal medicine active bactericidal insecticide; the multi-nutrition sterilizing and insecticidal blended fertilizer auxiliary material is obtained by uniformly mixing the Chinese herbal medicine active sterilizing insecticide and the decomposed organic fertilizer and granulating. The publication No. CN104761406B discloses a special fertilizer for scallion and a preparation method thereof, wherein the organic fertilizer is prepared from the following raw materials in parts by weight: 40-65 parts of livestock manure, 10-30 parts of peanut shell, 5-20 parts of bran, 5-20 parts of fresh garlic straw, 3-10 parts of fresh garlic bulb, 5-20 parts of waste Chinese medicine residue and 3-10 parts of traditional Chinese medicine components. Publication No. CN102134169A discloses a method for preparing a bio-organic fertilizer, which comprises the following components in percentage by weight: 20-50% of cow dung, 20-50% of rice straw, 0-50% of garlic straw, 1% of gypsum powder and 1-2% of lime powder. The publication No. CN108002955A discloses a saline-alkali soil improving fertilizer capable of preventing diseases and insect pests and a preparation method thereof, wherein the saline-alkali soil improving fertilizer is prepared from the following raw materials in parts by weight: 10-20 parts of tobacco leaves, 10-20 parts of garlic straws, 20-50 parts of excrement, 10-30 parts of plant straws (containing garlic straws), 10-15 parts of seaweed powder, 15-30 parts of zeolite and 15-20 parts of radix sophorae flavescentis10-15 parts of monopotassium phosphate, 5-10 parts of magnesium sulfate, 0.5-1 part of yeast, 0.3-1 part of bacillus and 50-100 parts of domestic sludge. Publication No. CN107892623A discloses a bio-organic fertilizer for increasing the mineral content of rice, which is prepared from the following raw materials: sheep manure, pig manure, composite biological bacteria, garlic straw, tomato seed meal, diatomite, borax, copper acetate, potassium humate and an additive. Publication No. CN107698342A discloses a preparation method of a biogas slurry nutritional agent for improving bioactive components of roses, which specifically comprises the following steps: (1) weighing raw materials, namely biogas slurry, grape skin residues, fresh sweet potato vine leaves, fresh mulberry leaves, garlic straws, morinda officinalis, rehmannia, pediococcus pentosaceus and water in parts by weight; (2) obtaining fermented pulp; (3) obtaining fermentation liquor; (4) and carrying out magnetization treatment on the fermentation liquor to obtain the biogas slurry nutrient. Publication No. CN106977249A discloses a selenium-rich grape fungal fertilizer, which is prepared by performing microbial fermentation on garlic straws, chicken manure, astragalus mongholicus dregs, furfural residues, dried pine needles, eggshells and brown sugar to obtain a fermented biological fertilizer, and performing fungal planting conversion on the fermented biological fertilizer.
Through retrieval, no report related to bacterial fertilizer development is found, wherein garlic straws are used as a main fermentation substrate, and the Enterobacter ludwigii with antifungal activity is used as a fermentation strain.
Disclosure of Invention
In order to solve the problems of low-efficiency utilization of garlic straws and environmental pollution, the invention provides a liquid bacterial fertilizer which takes the garlic straws as a main fermentation substrate and uses Enterobacter ludwigii with antifungal activity and plant growth promotion as a fermentation strain and a preparation method thereof. The present invention uses Enterobacter ludwigiiEnterobacter ludwigiiB424, the preservation number of the strain is CGMCC No. 14915. The preservation unit of the strain is China general microbiological culture Collection center, and the address is as follows: west road No.1, north chen, north china, beijing, chaoyang district; the preservation date is as follows: 11 and 16 months in 2017.
Enterobacter ludwigii of the present inventionEnterobacter ludwigiiB424 is isolated from the rhizosphere soil of garlic field and the body surface and intestinal tract of garlic maggots, said Enterobacter ludwigiiEnterobacterludwigiiB424, the 16S rDNA sequence has a full length of 1444bp,
the technical scheme of the invention is as follows:
a liquid bacterial fertilizer for fermenting garlic straw by utilizing Enterobacter ludwigii comprises the following raw materials in parts by weight:
garlic straw, monopotassium phosphate, soybean meal, corn flour, glucose, yeast powder, water and Enterobacter ludwigii LB fermentation liquor.
The preparation method of the Enterobacter ludwigii LB fermentation liquor uses LB culture medium to culture the Enterobacter ludwigiiEnterobacterludwigiiB424, when OD600When the content is 0.6-0.8, the fermentation liquor of the Enterobacter ludwigii LB is obtained.
Further, when the garlic straws are fresh garlic straws, the liquid bacterial fertilizer for fermenting the garlic straws by using the enterobacter ludwigii comprises the following raw materials in parts by weight:
30-40 parts of fresh garlic straw, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn flour, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of enterobacter ludwigii LB fermentation liquor and 230 parts of water.
When the garlic straws are garlic straw dry powder, the liquid bacterial fertilizer for fermenting the garlic straws by using the enterobacter lodesignei comprises the following raw materials in parts by weight:
15-20 parts of garlic straw dry powder, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn flour, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of Lord Weixi enterobacter LB fermentation liquor and 250 parts of water.
In the invention, the garlic straw is the part of the whole garlic plant without garlic bulb and garlic sprout.
The preparation method of the liquid bacterial fertilizer for fermenting garlic straw by using the enterobacter ludwigii comprises the following specific steps:
(1) cultivation of Enterobacter ludwigii, OD using LB Medium600Ending the culture when the culture time is 0.6-0.8 to obtain the LB fermentation liquor of the Enterobacter ludwigii;
(2) grinding fresh garlic straws into slurry or directly adding water with equal weight parts of dry garlic straw powder, and then uniformly mixing the slurry with soybean meal, corn meal, glucose, yeast powder and the rest water to obtain a mixture;
(3) adding the mixture obtained in the step (2) into LB fermentation liquor of the Enterobacter ludwigii, and continuing fermentation for 15 days at 30 ℃ and 180 rpm/min;
(4) adding potassium dihydrogen phosphate into the fermentation product obtained in the step (3).
The invention has the following beneficial effects:
enterobacter ludwigii of the present inventionEnterobacterludwigiiB424 is separated from the rhizosphere soil of the garlic field and the body surface and intestinal tract of garlic maggots; the strain can adapt to the growth environment of garlic plants, degrade antibiotic substances in the garlic plants, produce antibacterial active compounds and secrete auxin. According to the invention, garlic maggot associated bacteria are used for fermenting the garlic straws, allelochemicals inhibiting the growth of other crops in the straws are degraded in the process, and compounds with antibacterial activity to various plant pathogenic bacteria and active compounds promoting the growth of plants are generated; meanwhile, the multifunctional liquid bacterial fertilizer with the functions of promoting crop growth and resisting diseases, which is produced by the invention, reduces the use of chemical fertilizers and pesticides in farmlands, improves the ecological environment of farmlands, and has great economic, social and ecological benefits.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
Embodiment 1 liquid bacterial fertilizer for fermenting garlic straw by using enterobacter ludwigii and preparation method thereof
The liquid bacterial fertilizer comprises the following raw materials in parts by weight:
30 parts of fresh garlic straw, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn meal, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of Enterobacter ludwigii LB fermentation liquor and 230 parts of water; the preparation method of the Enterobacter ludwigii LB fermentation liquor uses LB culture medium to culture the Enterobacter ludwigiiEnterobacter ludwigiiB424, when OD600When the fermentation liquor is 0.7, the LB fermentation liquor of the Enterobacter ludwigii is obtained;
the preparation method comprises the following steps:
(1) cultivation of Enterobacter ludwigii, OD using LB Medium600Ending the culture when the culture time is 0.7 to obtain the LB fermentation liquor of the Enterobacter ludwigii;
(2) grinding fresh garlic straws into slurry or directly adding water with equal weight parts of dry garlic straw powder, and then uniformly mixing the slurry with soybean meal, corn meal, glucose, yeast powder and the rest water to obtain a mixture;
(3) adding the mixture obtained in the step (2) into LB fermentation liquor of the Enterobacter ludwigii, and continuing fermentation for 15 days at 30 ℃ and 180 rpm/min;
(4) adding potassium dihydrogen phosphate into the fermentation product obtained in the step (3).
Said Enterobacter ludwigiiEnterobacter ludwigiiThe preservation number of B424 is CGMCC No.14915, the preservation unit is China general microbiological culture Collection center, and the address is China, No. 3 Siro No.1 of Beijing, Chaoyang district, Beicheng; the preservation date is 11 months and 16 days in 2017.
The 20 kg liquid bacterial manure obtained in the embodiment is applied to tomato seedlings in 3-5 leaf stage along with watering, the application area is 1 mu of land (667 square meters), after the application, the tomato incidence rate is reduced by 23.2 percent due to root diseases, and the tomato yield is increased by 5.7 percent.
Embodiment 2 liquid bacterial fertilizer for fermenting garlic straw by using enterobacter ludwigii and preparation method thereof
The liquid bacterial fertilizer comprises the following raw materials in parts by weight:
35 parts of fresh garlic straw, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn meal, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of Enterobacter ludwigii LB fermentation liquor and 230 parts of water;
the preparation method of the Enterobacter ludwigii LB fermentation liquor uses LB culture medium to culture the Enterobacter ludwigiiEnterobacter ludwigiiB424, when OD600When the fermentation liquor is 0.6, the LB fermentation liquor of the Enterobacter ludwigii is obtained;
the preparation method comprises the following steps:
(1) cultivation of Enterobacter ludwigii, OD using LB Medium600Ending the culture when the culture time is 0.6 to obtain the LB fermentation liquor of the Enterobacter ludwigii;
(2) grinding fresh garlic straws into slurry or directly adding water with equal weight parts of dry garlic straw powder, and then uniformly mixing the slurry with soybean meal, corn meal, glucose, yeast powder and the rest water to obtain a mixture;
(3) adding the mixture obtained in the step (2) into LB fermentation liquor of the Enterobacter ludwigii, and continuing fermentation for 15 days at 30 ℃ and 180 rpm/min;
(4) adding potassium dihydrogen phosphate into the fermentation product obtained in the step (3).
Said Enterobacter ludwigiiEnterobacter ludwigiiThe preservation number of B424 is CGMCC No.14915, the preservation unit is China general microbiological culture Collection center, and the address is China, No. 3 Siro No.1 of Beijing, Chaoyang district, Beicheng; the preservation date is 11 months and 16 days in 2017.
The 20 kg liquid bacterial manure obtained in the embodiment is applied to tomato seedlings in 3-5 leaf stage along with watering, the application area is 1 mu of land (667 square meters), after the application, the tomato incidence rate is reduced by 21.1 percent due to root diseases, and the tomato yield is increased by 6.2 percent.
Embodiment 3 liquid bacterial fertilizer for fermenting garlic straw by using enterobacter ludwigii and preparation method thereof
The liquid bacterial fertilizer comprises the following raw materials in parts by weight:
40 parts of fresh garlic straw, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn meal, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of Enterobacter ludwigii LB fermentation liquor and 230 parts of water;
the preparation method of the Enterobacter ludwigii LB fermentation liquor uses LB culture medium to culture the Enterobacter ludwigiiEnterobacter ludwigiiB424, when OD600When the fermentation liquor is 0.8, the LB fermentation liquor of the Enterobacter ludwigii is obtained;
the preparation method comprises the following steps:
(1) cultivation of Enterobacter ludwigii, OD using LB Medium600Ending the culture when the culture time is 0.8 to obtain the LB fermentation liquor of the Enterobacter ludwigii;
(2) grinding fresh garlic straws into slurry or directly adding water with equal weight parts of dry garlic straw powder, and then uniformly mixing the slurry with soybean meal, corn meal, glucose, yeast powder and the rest water to obtain a mixture;
(3) adding the mixture obtained in the step (2) into LB fermentation liquor of the Enterobacter ludwigii, and continuing fermentation for 15 days at 30 ℃ and 180 rpm/min;
(4) adding potassium dihydrogen phosphate into the fermentation product obtained in the step (3).
Said Enterobacter ludwigiiEnterobacter ludwigiiThe preservation number of B424 is CGMCC No.14915, the preservation unit is China general microbiological culture Collection center, and the address is China, No. 3 Siro No.1 of Beijing, Chaoyang district, Beicheng; the preservation date is 11 months and 16 days in 2017.
The 20 kg liquid bacterial manure obtained in the embodiment is applied to tomato seedlings in 3-5 leaf stage along with watering, the application area is 1 mu of land (667 square meters), after the application, the tomato incidence rate is reduced by 22.4 percent due to root diseases, and the tomato yield is increased by 5.8 percent.
Embodiment 4 liquid bacterial fertilizer for fermenting garlic straw by using enterobacter ludwigii and preparation method thereof
The liquid bacterial fertilizer comprises the following raw materials in parts by weight:
15 parts of garlic straw dry powder, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn meal, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of Louweixiella LB fermentation liquor and 250 parts of water;
the preparation method of the Enterobacter ludwigii LB fermentation liquor uses LB culture medium to culture the Enterobacter ludwigiiEnterobacter ludwigiiB424, when OD600When the fermentation liquor is 0.6, the LB fermentation liquor of the Enterobacter ludwigii is obtained;
the preparation method comprises the following steps:
(1) cultivation of Enterobacter ludwigii, OD using LB Medium600Ending the culture when the culture time is 0.6 to obtain the LB fermentation liquor of the Enterobacter ludwigii;
(2) grinding fresh garlic straws into slurry or directly adding water with equal weight parts of dry garlic straw powder, and then uniformly mixing the slurry with soybean meal, corn meal, glucose, yeast powder and the rest water to obtain a mixture;
(3) adding the mixture obtained in the step (2) into LB fermentation liquor of the Enterobacter ludwigii, and continuing fermentation for 15 days at 30 ℃ and 180 rpm/min;
(4) adding potassium dihydrogen phosphate into the fermentation product obtained in the step (3).
Said Enterobacter ludwigiiEnterobacter ludwigiiThe preservation number of B424 is CGMCC No.14915, the preservation unit is China general microbiological culture Collection center, and the address is China, No. 3 Siro No.1 of Beijing, Chaoyang district, Beicheng; the preservation date is 11 months and 16 days in 2017.
The 20 kg liquid bacterial manure obtained in the embodiment is applied to 3-5 leaf stage corn seedlings along with watering, the application area is 1 mu of land (667 square meters), after the application, the occurrence rate of the corn diseases at the roots is reduced by 10.3%, and the yield is increased by 8.2%. Embodiment 5 liquid bacterial fertilizer for fermenting garlic straw by using enterobacter ludwigii and preparation method thereof
The liquid bacterial fertilizer comprises the following raw materials in parts by weight:
20 parts of garlic straw dry powder, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn meal, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of enterobacter ludwigii LB fermentation liquor and 250 parts of water;
the preparation method of the Enterobacter ludwigii LB fermentation liquor uses LB culture medium to culture the Enterobacter ludwigiiEnterobacter ludwigiiB424, when OD600When the fermentation liquor is 0.7, the LB fermentation liquor of the Enterobacter ludwigii is obtained;
the preparation method comprises the following steps:
(1) cultivation of Enterobacter ludwigii, OD using LB Medium600Ending the culture when the culture time is 0.7 to obtain the LB fermentation liquor of the Enterobacter ludwigii;
(2) grinding fresh garlic straws into slurry or directly adding water with equal weight parts of dry garlic straw powder, and then uniformly mixing the slurry with soybean meal, corn meal, glucose, yeast powder and the rest water to obtain a mixture;
(3) adding the mixture obtained in the step (2) into LB fermentation liquor of the Enterobacter ludwigii, and continuing fermentation for 15 days at 30 ℃ and 180 rpm/min;
(4) adding potassium dihydrogen phosphate into the fermentation product obtained in the step (3).
Said Enterobacter ludwigiiEnterobacter ludwigiiThe preservation number of B424 is CGMCC No.14915, the preservation unit is China general microbiological culture Collection center, and the address is China, No. 3 Siro No.1 of Beijing, Chaoyang district, Beicheng; the preservation date is 11 months and 16 days in 2017.
Said Enterobacter ludwigiiEnterobacter ludwigiiB424, the preservation number of the strain is CGMCC No.14915, and the strain is preserved in the China general microbiological culture Collection center.
The 20 kg liquid bacterial manure obtained in the embodiment is applied to a wheat field in a tillering stage along with watering, the application area is 1 mu (667 square meters), after the application, the disease incidence rate of the root in the later stage of the wheat is reduced by 13.7%, and the yield is increased by 6.2%.

Claims (7)

1. A liquid bacterial fertilizer for fermenting garlic straws by utilizing Enterobacter ludwigii is characterized by comprising the following raw materials:
garlic straw, monopotassium phosphate, soybean meal, corn flour, glucose, yeast powder, water and Enterobacter ludwigii LB fermentation liquor;
the Enterobacter ludwigii is Enterobacter ludwigii B424, the preservation number of the strain is CGMCC No.14915,
the preservation unit of the strain is China general microbiological culture Collection center, and the address is as follows: west road No.1, north chen, north china, beijing, chaoyang district; the preservation date is as follows: 11/2017 and 16/month;
the liquid bacterial manure is applied with watering.
2. The liquid bacterial fertilizer of claim 1, wherein the preparation method of the LB fermentation liquid of the Enterobacter ludwigii comprises the step of culturing Enterobacter ludwigii B424 by using LB culture medium, when OD is obtained600When the content is 0.6-0.8, the fermentation liquor of the Enterobacter ludwigii LB is obtained.
3. The liquid bacterial fertilizer of claim 2, wherein the preparation method of the LB fermentation liquid of the Enterobacter ludwigii comprises the step of culturing the Enterobacter ludwigii B424 by using an LB culture medium, when OD is obtained600When the fermentation time is 0.7, the LB fermentation liquor of the Enterobacter ludwigii is obtained.
4. The liquid bacterial fertilizer as claimed in claim 2, wherein when the garlic straw is fresh garlic straw, the liquid bacterial fertilizer for fermenting the garlic straw by using the enterobacter ludwigii comprises the following raw materials in parts by weight:
30-40 parts of fresh garlic straw, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn flour, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of enterobacter ludwigii LB fermentation liquor and 230 parts of water.
5. The liquid bacterial fertilizer of claim 2, wherein when the garlic straw is garlic straw dry powder, the liquid bacterial fertilizer for fermenting the garlic straw by using the enterobacter ludwigii comprises the following raw materials in parts by weight:
15-20 parts of garlic straw dry powder, 15 parts of monopotassium phosphate, 10 parts of soybean meal, 6 parts of corn flour, 1.5 parts of glucose, 0.6 part of yeast powder, 50 parts of Lord Weixi enterobacter LB fermentation liquor and 250 parts of water.
6. The liquid bacterial fertilizer as claimed in claim 1, wherein the garlic straw is a part of whole garlic plant with garlic bulb and garlic sprout removed.
7. A method for preparing the liquid bacterial manure as claimed in any one of claims 1-6, comprising the following steps:
(1) cultivation of Enterobacter ludwigii, OD using LB Medium600Ending the culture when the culture time is 0.6-0.8 to obtain the LB fermentation liquor of the Enterobacter ludwigii;
(2) grinding fresh garlic straws into slurry or directly adding water with equal weight parts of dry garlic straw powder, and then uniformly mixing the slurry with soybean meal, corn meal, glucose, yeast powder and the rest water to obtain a mixture;
(3) adding the mixture obtained in the step (2) into LB fermentation liquor of the Enterobacter ludwigii, and continuing fermentation for 15 days at 30 ℃ and 180 rpm/min;
(4) adding potassium dihydrogen phosphate into the fermentation product obtained in the step (3).
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