CN102579597B - Chinese herbal compound prescription for preventing and curing mammitis of mammalian livestock and method for preparing same - Google Patents

Chinese herbal compound prescription for preventing and curing mammitis of mammalian livestock and method for preparing same Download PDF

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CN102579597B
CN102579597B CN 201210086366 CN201210086366A CN102579597B CN 102579597 B CN102579597 B CN 102579597B CN 201210086366 CN201210086366 CN 201210086366 CN 201210086366 A CN201210086366 A CN 201210086366A CN 102579597 B CN102579597 B CN 102579597B
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cow
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CN102579597A (en
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伍清林
金兰梅
金保方
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Jinling Institute of Technology
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Abstract

The invention discloses a Chinese herbal compound prescription for preventing and curing mammitis of mammalian livestock, which comprises the following components in parts by weight: 30-60 parts of dandelion, 20-50 parts of semenvaccariae, 30-60 parts of astragali radix, 30-60 parts of akebia quinata, 10-30 parts of fructus forsythiae, 10-30 parts of spina gleditsiae, 10-30 parts of beautiful sweetgum fruit, 10-30 parts of radix bupleuri, 10-30 parts of uniflower swisscentaury root and 3-5 parts of liquorice. The invention also discloses a method for preparing the Chinese herbal compound prescription. The Chinese herbal compound prescription has an obvious therapeutic effect on curing the clinical mastitis of dairy cattle and goats, substitutes for antibiotic during the drug use process, and does not have residue in cow milk and goat milk, so the production of high quality milk is promoted. The Chinese herbal compound prescription cures the recessive mastitis of the dairy cattle and can reduce the somatic cell count in the cow milk, so the milk production is improved, and the milk quality is not influenced. The Chinese herbal compound prescription can prevent the mammitis of the dairy cattle or goats when being applied in healthy secretion dairy cattle or goats, so the milk production is improved.

Description

A kind of Chinese medicine compound of preventing and treating the mammals garget and preparation method thereof
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of Chinese medicine compound of preventing and treating the mammals garget and preparation method thereof.
Background technology
Mammitis of cow is that mammary gland is subjected to a kind of inflammatory variation that physics, chemistry and microorganism stimulation take place, and somatic cell content increases in the milk, and mammary gland tissue generation pathological change is one of regular incidence of cattle farm, causes enormous economic loss.Mammitis of cow divides 2 kinds of clinic mastitis and latent mammitises.The former occurring degree is serious, and sickness rate is low, though latent mammitis does not have clinical type performance, but can detect the positive with CMT, and antibacterial separates can find pathogen, and the sickness rate height is difficult for curing.It is reported that the sickness rate of the U.S., Japan is 62.11%, China is 73.19%, and latent mammitis can make milk yield descend 10%~15%, and newborn quality descends, and what have also changes into clinic mastitis.This disease not only causes heavy losses to aquaculture, and also brings no small harm for human beings'health, and control that therefore should disease seems particularly important.
The traditional treatment measure of mastitis is anti-inflammation, improves environment, strengthens the resistance of animal body, uses various chemicals to treat by breast part and whole body administration for a long time.Along with the long-term a large amount of use of antibiotic, cause problems such as the resistance of common concern and antibiotic remains.Select newtype drug to prevent and treat milch cow even other mammals gargets, become the important topic that the milk industry develops in a healthy way.Chinese medicine preparation treatment mastitis has short treating period, instant effect, characteristics such as economical and practical.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Chinese medicine compound of preventing and treating the mammals garget.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned Chinese medicine compound.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of Chinese medicine compound of preventing and treating the mammals garget, it comprises the component of following parts by weight: 30~60 parts of Herba Taraxacis, 20~50 parts of SEMEN VACCARIAE, 30~60 parts of Radix Astragali, 30~60 parts of Caulis Akebiaes, 10~30 parts of Fructus Forsythiaes, 10~30 parts of Spina Gleditsiaes, 10~30 parts of Fructus Liquidambaris, 10~30 parts of Radix Bupleuri, 10~30 parts of Radix Rhapontici, 3~5 parts in Radix Glycyrrhizae.
Wherein, described mammals domestic animal is cattle or sheep.
First kind of preparation method of above-mentioned Chinese medicine compound, Herba Taraxaci, SEMEN VACCARIAE, Radix Astragali, Caulis Akebiae, Fructus Forsythiae, Spina Gleditsiae, Fructus Liquidambaris, Radix Bupleuri, Radix Rhapontici and Radix Glycyrrhizae difference dryness in the sun with formula ratio, again respectively after oven dry under 50~60 ℃, all the other prescription mixing pulverizing except SEMEN VACCARIAE, cross 60 mesh sieves, to all fill a prescription again and pulverize again after mixing, cross 100 mesh sieves, namely.
Second kind of preparation method of above-mentioned Chinese medicine compound, Herba Taraxaci, SEMEN VACCARIAE, Radix Astragali, Caulis Akebiae, Fructus Forsythiae, Spina Gleditsiae, Fructus Liquidambaris, Radix Bupleuri, Radix Rhapontici and Radix Glycyrrhizae dryness in the sun with formula ratio, add the water logging bubble of 10 times of weight, decoct 2 times merging filtrate, make decoct.
The application of above-mentioned Chinese medicine compound in preparation control mammals domestic animal acute mastitis medicine.
The application of above-mentioned Chinese medicine compound in preparation control mammals domestic animal latent mammitis medicine.
The Chinese medicine compound using dosage and the usage that adopt first kind of preparation method to obtain are: lactating cow 100~200g/ days/head, suffer from 200-300g/ days/head of latent mammitis milch cow, and sneak in the feedstuff, search for food voluntarily.30~80g/ days/head of lactogenic goat is sneaked in the feedstuff, searches for food voluntarily.
The Chinese medicine compound using dosage and the usage that adopt second kind of preparation method to obtain are: the former medicine of ill milch cow 300~500g Chinese medicine/sky/head, make decoct, and throw something and feed.The former medicine of ill goat 100~150g Chinese medicine/sky/head is made decoct, throws something and feeds.
Chinese veterinarian thinks that mastitis is acute mastitis, be ache, wet, gas, blood stagnation knot do not loose, change and be scorching.Because of the damp and hot ground of lying on bed for a long time, steam on the damp and hot poison, encroach on breast, or because of excessiveness of stomach-heat, stagnation of QI due to depression of the liver, newborn network lose smooth down to the breast coagulation of QI-blood, the stasis of blood is tied and living carbuncle; Or the nipple that bites when inhaling breast because of calf, evil poison invasion causes carbuncle; Or suffer from the cattle refusal and milk milk is stagnated, breast overflows etc. are former thereby cause primary disease, thus the prescription of Chinese medicinal formulae should be with heat-clearing and toxic substances removing, anti-inflammation, restore menstrual flow and invigorate blood circulation, active lactogenesis is principle.With heat and toxic materials clearing away medicine, blood circulation and stasis-eliminating medicine, diaphoretic medicine and damp-excreting medicinal, medicine such as be aided with tonification simultaneously, regulate the flow of vital energy, thus reach the purpose for the treatment of mammitis of cow with tonifying Qi and lifting yang, strengthening vital QI to eliminate pathogenic factors.Herba Taraxaci, Fructus Forsythiae, Radix Bupleuri have the merit of heat-clearing and toxic substances removing, antibiotic, antiinflammatory, dispersing swelling and dissipating binds in the Chinese medicinal formulae; Radix Astragali QI invigorating tonify deficiency can obviously improve hematid immunity function, neutrophilic granulocyte phagocytic power etc.; Spina Gleditsiae, Radix Rhapontici detumescence and apocenosis, lactogenesis; Caulis Clematidis Armandii, SEMEN VACCARIAE have the effect of the eliminating stagnation of regulating the flow of vital energy, active stimulating milk secretion; The Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription helps the Radix Astragali with the QI invigorating tonify deficiency.All medicines cooperate the merit with heat-clearing and toxic substances removing, anti-inflammation, detumescence stimulating milk secretion.
Beneficial effect:
Chinese medicine compound of the present invention takes place to have the obvious treatment effect when clinical mastitis is treated milch cow and goat, has substituted antibiotic usefulness in the medication process, and Lac Bovis seu Bubali and the noresidue of goat Ruzhong have promoted the production of high-quality milk.The treatment milch cow can reduce the somatic number in the Lac Bovis seu Bubali, improves milk yield, and does not influence the quality of breast.In healthy cow in milk or goat, use the mastitis that can prevent milch cow or goat, improve milk yield.
The specific embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that the described content of embodiment only is used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
A kind of Chinese medicine compound of preventing and treating the mammals garget, it comprises the component of following parts by weight:
30 parts of Herba Taraxacis, 20 parts of SEMEN VACCARIAE, 30 parts of Radix Astragali, Caulis Akebiae 30g part, Fructus Forsythiae 10g part, 10 parts of Spina Gleditsiaes, 10 parts of Fructus Liquidambaris, 10 parts of Radix Bupleuri, 10 parts of Radix Rhapontici, 3 parts in Radix Glycyrrhizae.
Preparation method: with Herba Taraxaci, SEMEN VACCARIAE, Radix Astragali, Caulis Akebiae, Fructus Forsythiae, Spina Gleditsiae, Fructus Liquidambaris, Radix Bupleuri, Radix Rhapontici and the Radix Glycyrrhizae difference dryness in the sun of formula ratio, again respectively after oven dry under 50~60 ℃, all the other prescription mixing pulverizing except SEMEN VACCARIAE, cross 60 mesh sieves, to all fill a prescription again and pulverize again after mixing, cross 100 mesh sieves, namely.
Embodiment 2:
A kind of Chinese medicine compound of preventing and treating the mammals garget, it comprises the component of following parts by weight:
60 parts of Herba Taraxacis, 50 parts of SEMEN VACCARIAE, 60 parts of Radix Astragali, Caulis Clematidis Armandii 60g part, Fructus Forsythiae 30g part, 30 parts of Spina Gleditsiaes, 30 parts of Fructus Liquidambaris, 30 parts of Radix Bupleuri, 30 parts of Radix Rhapontici, 5 parts in Radix Glycyrrhizae.
Preparation method: with Herba Taraxaci, SEMEN VACCARIAE, Radix Astragali, Caulis Akebiae, Fructus Forsythiae, Spina Gleditsiae, Fructus Liquidambaris, Radix Bupleuri, Radix Rhapontici and the Radix Glycyrrhizae dryness in the sun of formula ratio, add the water logging bubble of 10 times of weight, decoct 2 times merging filtrate, make decoct.
Embodiment 3:
A kind of Chinese medicine compound of preventing and treating the mammals garget, it comprises the component of following parts by weight:
40 parts of Herba Taraxacis, 35 parts of SEMEN VACCARIAE, 50 parts of Radix Astragali, Caulis Clematidis Armandii 40g part, Fructus Forsythiae 20g part, 15 parts of Spina Gleditsiaes, 20 parts of Fructus Liquidambaris, 25 parts of Radix Bupleuri, 20 parts of Radix Rhapontici, 4 parts in Radix Glycyrrhizae.
Preparation method is with embodiment 1.
Embodiment 4: compound Chinese medicinal preparation is to the observation of curative effect of bovine subclinical mastitis.
1 experiment cattle is selected and grouping:
From Nanjing Wei Gang dairy industry company limited Xi Gang joint operation pasture lactogenic cows, select no mastitis clinical symptoms, somatic number is greater than 500,000 in the milk, it is positive that CAM detects, and antibacterial is separated to 60 of the milch cows of pathogen, identical according to age, parity, lactogenic day similar (calving date is in 5d), close principle was matched and was divided into groups milk yield and lactation period, and 30 are the treatment group, and 30 is matched group, the treatment group is fed at twice with Chinese medicinal formulae (embodiment 1 prescription) 200g/d/ head.Not medication of matched group.It is standby to each group oxtail radicular vein blood sampling separation of serum respectively to test forward and backward 7d, 14d, 21d, 30d, tests forward and backward 7d, 14d, 21d, 30d, 60d, 90d, 120d and gathers newborn sample for three times to mix the back standby the test cattle is early, middle and late respectively.
The feeding and management of 2 test cattle:
Tested on April 6th, 2011 and carried out to May 6, preliminary trial period is 7d, and experimental period is 30d, and 60 cow heads concentrate on centralized and unified feeding and management in the double row cowshed.The test cattle is identical other cows of feeding and management method and pasture of duration of test.The feeding manner of this milch cow is for tying formula, the mixed fodder of throughout the year feeding (silagecorn, distiller's grains of beer, sugar are poor, apple pomace, Herba Medicaginis grass piece, Herba Medicaginis granule, Herba Medicaginis bale, Semen Gossypii, corn, soybean cake, wheat bran, bone meal etc.).Groove is three times on every day, and the time is respectively 8:00,14:00,20:00, and Chinese medicine compound (embodiment 1) is placed in the concentrate at 8:00 and 20:00.Every day, the machine milking was 3 times, and the time is respectively 8:00,16:00,22:00.The milk yield of each each time of computer record.
3 test events and method of testing:
Production target is measured: the milk yield of every cattle of record every day.Each group is gathered newborn sample, uses somatic cell, butterfat and lactoprotein in the FOSS milk infrared analysis scanner mensuration milk at Wei Gang dairy industry company limited quality inspection center, Nanjing.
Index determinings such as enzyme, free radical, pro-inflammatory cytokine in the serum: lactic acid dehydrogenase (LDH), serum alkaline phosphatase (ALP), glutamate pyruvate transaminase (GPT), superoxide dismutase (SOD), catalase (CAT); Malonaldehyde (MDA), nitric oxide (NO), Total antioxidant capacity (T-AOC); Interleukin 8 (IL-8), tumor necrosis factor-alpha (TNF-α) etc., each test event is all by the strict operation of test kit explanation.
The test kit box all builds up bio-engineering research institute available from Nanjing.
4, Chinese medicine compound is to the influence of latent mammitis milk production of cow and newborn quality:
Use Chinese medicinal formulae treatment bovine subclinical mastitis, before test and when test back 14,21d, the matched group milk production of cow reduces 2.5kg/ head/d, 7.3kg/ head/d respectively before testing, compare with the treatment group and to have reduced 0.9kg/ head/d, 4.4kg/ head/d respectively, the treatment group then keeps relative stability.Comparing difference remarkable (P<0.05) before medication the 21st, 30d matched group and treatment group and test; Poor heteropole remarkable (P<0.01) before test back 60,90,120d matched group and treatment group and the test, the latent mammitis milch cow is used compound of Chinese medicine of the present invention, and milk yield improves 12.01% during medication.Illustrate that Chinese medicinal formulae has therapeutical effect to bovine subclinical mastitis, have the milch cow of enhancing milk performance simultaneously, improve milk yield, and have long after effect.The results are shown in Table 1.
Table 1 Chinese medicine compound of the present invention is to kg/ the n=30 that influence of latent mammitis milk production of cow
Figure BDA0000147757440000051
Annotate: the different capitalizations of same row, column data shoulder mark are represented difference extremely significantly (P<0.01) in the table, the different lowercase alphabet differentials of same column data shoulder mark different significantly (P<0.05)
As shown in Table 2, butterfat percnetage before and after test in the dairy cow milk and lactoprotein do not have significance to change, the treatment group is before 60d, 90d, 120d lactoprotein are higher than medication after medication, butterfat percnetage is (P<0.05) before 90d is higher than medication, illustrate that Chinese medicinal formulae can improve milk yield, and do not influence the quality of breast.
Butterfat, content of milk protein measurement result (%) n=30 in table 2 dairy cow milk
Annotate: same delegation in the table, the different lowercase alphabet differentials of same column data shoulder mark different significantly (P<0.05)
5, Chinese medicine compound is to the somatic influence of latent mammitis dairy cow milk:
As shown in Table 3, before test, the somatic cell in 2 groups of dairy cow milks does not have difference, and the somatic cell in the 14d milk after medication for the treatment of group significantly descends (P<0.01), drops to 500,000/ml during 21d, maintains in the normal level during to 120d always.Matched group keeps higher level always, and the trend of rising is arranged behind 90d.It can be said that bright Chinese medicinal formulae can make somatic number minimizing in the lactating cow milk, effectively treats bovine subclinical mastitis.
Somatic cell assay result in table 3 dairy cow milk
Annotate: the different capitalizations of same row, column data shoulder mark are represented difference extremely significantly (P<0.01) in the table.
6, Chinese medicine compound influences indexs such as the enzyme in the latent mammitis serum, free radicals:
LDH can be used as the index of mastitis early diagnosis.In tentative mastitis pathogenic process, time, term, lactogenic stage and milk yield before and after the diagnosis all influence the LDH activity significantly.As shown in Table 4, the active indifference opposite sex (P>0.05) of LDH before two groups of milch cow tests, 7 days LDH activity decrease after the medication for the treatment of group, but compare difference with matched group not remarkable, reduced 756.95U/L after the medication in 14 days, compare with matched group and to have reduced 557.07U/L, reduced 1597.89U/L in 21 days, compare with matched group and reduced 1632.71U/L; Significant difference (P<0.05).And the no significant change (P>0.05) before and after test of matched group LDH activity.Prompting has had tangible reduction with LDH activity behind the compound of Chinese medicine mastitis of the present invention.Show the trend that mastitis take a favorable turn.
The activity of ALP has obvious change in numerous disease, the ALP activity increases during bacterial infection, therefore, often assists to differentiate and infect character that there is bacterial infection in the activity of ALP rising indication mastitis milch cow in the mastitis cow serum clinically with the activity of ALP.Treatment group milch cow 14 days ALP activity after medication have reduced by 9.61 Jin Shi U/mL, compare with matched group and have reduced by 13.42 Jin Shi U/mL, reduce by 11.61 Jin Shi U/Ml in 21 days, compare with matched group and have reduced by 18.32 Jin Shi U/mL, significant difference (P<0.05).No significant change (P>0.05) before and after the test of matched group milch cow.Illustrate that Chinese medicine compound of the present invention has inhibitory action to the pathogenic bacterium in the mastitis dairy cow milk.
GPT is the important amino acid transaminase that extensively is present in the animal mitochondria, plays an important role in the protein metabolism in vivo, is hepatocyte the sensitiveest impaired index, can be so as to estimating the toxic and side effects of medicine.Result of the test shows that after the latent mammitis milch cow was used Chinese medicine compound of the present invention, serum GPT did not have significant change (P>0.05), illustrates that Chinese medicine compound of the present invention has no side effect to the milch cow body.
SOD almost is present in all biologies by the oxygen breathing; it can remove superfluous oxygen-derived free radicals; the chain reaction of blocking-up Mediated by Free Radicals; because mastitis intracellular reactive oxygen has participated in the cell injury effect; the oxidative stress status of cell changes; cell is in order to protect the infringement of oneself avoiding free radical, then increase antioxidase SOD etc., thus reduce degree of tissue damage [6]This test shows, treatment group milch cow is the 7th day active rising 10.3U/mL of SOD after the medication, compare the 9.41U/mL that raise (P<0.05) with matched group, the 14th day SOD activity 18.91U/mL that raise after the medication, compare the 18.77U/mL that raise with matched group, 21 days 24.67U/mL that raise compare the 23.11U/mL that raise with matched group, (P<0.05), the no significant change (P>0.05) before and after test of matched group milch cow.Illustrate that Chinese medicine compound of the present invention can significantly improve mastitis cow serum SOD vigor, improve antioxidant ability of organism, the immunity of enhancing body.
CAT is the very important anti-oxidative defense sexual function enzyme that exists in the organism, in the anti-oxidative defense system of organism, occupy critical role, mainly be combined with the mistake oxysome, energy is the harmful substance hydrogen peroxide of scavenger cell metabolism generation rapidly, form to reduce free radical, the CAT activity increases the explanation antioxidant ability of organism and increases.This test shows, treatment group milch cow CAT 1.98U/mL that raise the 14th day serum after medication compares the 1.28U/mL that raise with matched group, and 21 days 3.28U/mL that raise compare the 2.91U/mL that raise with matched group, significant difference (P<0.05).The no significant change (P>0.05) before and after test of matched group milch cow.Show that Chinese medicine compound of the present invention has the trend that improves mastitis cow serum CAT vigor, the ability of the anti-damage of enhancing body.
MDA is intensity and the speed of reflection oxygen-derived free radicals level and lipid peroxidation, free radical metabolic alterations in vivo, and free radical causes the important indicator of body tissue cell injury.The size that how much has shown the lipid peroxidation degree of MDA content in the body can be used as what indicant of free-radical contents, also can the biomembranous extent of damage of indirect reaction.As can be seen from Table 4 in the mastitis cow serum content of MDA than healthy cow height, originally studies show that, the content of MDA descends after the latent mammitis milch cow use Chinese medicine compound of the present invention, 1.91U/mL has descended before the ratio test in 14 days after the medication, than 2 groups of 1.15U/mL that descended of test, 21 days than the 3.27U/mL that descended before the test, than 2 groups of 2.9U/mL that descended of test, significant difference (P<0.05), and the content of testing 2 groups of MDA does not have significant change (P>0.05) all the time.Show that Chinese medicine compound of the present invention can reduce free radical and cause the body tissue cells injury.
Table 4 Chinese medicine compound is to the n=30 that influences of related enzyme activity and free radical in the latent mammitis cow serum
Figure BDA0000147757440000071
Annotate: represent significant difference (P<0.05) with the different capitalizations of data line shoulder mark in the table, the different minuscules of same column data shoulder mark are represented significant difference (P<0.05).
Many result of the tests confirm that the inflammatory phase pro-inflammatory cytokine stimulates induction type NO synthase (iNOS), and synthetic a large amount of NO promotes inflammatory reaction.The overexpression of iNOS causes NO and produces and often inspire the immunopathogenesis process when excessive and cause tissue injury.NO generates the important promoting factor that too much becomes numerous disease.The content of NO is than healthy cow height in the mastitis cow serum, can learn two groups of preceding NO content zero differences of latent mammitis milch cow test by table 6,7 days NO content descends to some extent after the medication for the treatment of group, but difference is remarkable (P>0.05) not, prolongation along with the time, medication group NO content significantly reduces, 14 days 1.84 μ moL/mL that descend, 21 days 3.16 μ moL/mL (P<0.05) that descend.There is not significant change and test 2 groups of milch cow NO content.The decline of NO numerical value further illustrates Chinese medicine compound of the present invention and has played certain effect to removing the free radical aspect.
T-AOC is the fly up and down main system of oxygen-derived free radicals of body, restriction and remove body and produce too much free radical, and protection cell normal function is kept the homergy of body.As can be seen from Table 4, T-AOC began to raise 7 days 0.95U/mL that raise, 14 days 1.7U/mL that raise, 21 days 2.61U/mL that raise, significant difference (P<0.05) after the mastitis milch cow was drunk Chinese medicine compound of the present invention.Compare 7 days 1.08U/mL that raise, 14 days 2.1U/mL that raise, 21 days 2.71U/mL that raise, significant difference (P<0.05) for 2 groups with test.Illustrate that Chinese medicine compound of the present invention can improve the Total antioxidant capacity of body, make the interior free radical of body be eliminated, the mastitis symptom is alleviated also disappearance.
7, Chinese medicinal formulae is to the influence of pro-inflammatory cytokine in the latent mammitis serum:
IL-8 polymorphonuclear leukocyte (PMN), basophilic granulocyte and the isocellular chemotactic factor of T cell have important function in inflammatory reaction.This test shows, IL-8 also increases gradually with the aggravation content of inflammation, this mainly is because when mammary gland is inflamed, level of endotoxin increases and stimulates cytokines such as inflammatory cell release IL-1, TNF-α in the serum, induce down in the stimulation of these cytokines, various kinds of cell such as mononuclear cell and PMN all can produce IL-8, and this result shows that the IL-8 in the serum helps the development of body inflammatory.TNF-α participates in the generation of inflammatory reaction and aggravates inflammation.
IL-8, the rising of TNF-alpha content in blood when mastitis takes place milch cow, 14d begins to descend after using Chinese medicinal formulae, with before the test and matched group than difference remarkable (P<0.05), the treatment group is before medication 21d and 30d and test and matched group ratio heteropole significantly remarkable (P<0.01).See Table 5.
Table 5 Chinese medicine compound is to the n=30 that influences of pro-inflammatory cytokine in the latent mammitis cow serum
Figure BDA0000147757440000081
Annotate: represent difference extremely significantly (P<0.01) with the different capitalizations of data line shoulder mark in the table, the different minuscules of same column data shoulder mark are represented significant difference (P<0.05)
8, therapeutic effect and Economic and Efficiency Analysis:
Clinical mastitis constantly takes place in the lactational cows in this cattle farm of duration of test, matched group 30 cow heads had 2 cow head generation mastitiss from May 2, milk yield is sharply descended, 5, June, the DHI testing result showed, Ruzhong somatic cell number average is more than 500,000/ml, in 3 months after drug withdrawal, there are 3 hairs to give birth to clinical mastitis again, the sickness rate of 3 months matched group mammitis of cow is 16.67% (5/30) after from the on-test to the drug withdrawal, treatment is organized has 26 somatic numbers to drop in 500,000/ml in 30 cow heads, clinical mastitis does not take place in 500,000/ml in 4 head heights.Illustrate that treatment has effect preferably to Chinese medicinal formulae to bovine subclinical mastitis.
The mixed fodder that this milch cow is fed, lactation period, the mixed fodder total amount of every cow head was approximately 29kg, and unit price is 2.20 yuan/kg, 33.6 yuan of kg of Chinese medicinal formulae, the fresh milk price is 3.90 yuan/kg.
Calculate according to table 1, duration of test treatment group and matched group milk yield are 26.58kg/ head/d, 23.73kg/ head/d, the treatment group is than the voluminous milk of matched group 2.85kg head/d, increase by 11.12 yuan/head/d of milk income, deduct 6.72 yuan of the cost prices of Chinese medicine, increase by 4.40 yuan/head/d of net income, increase economic efficiency 12.97%, the Economic and Efficiency Analysis during the medication sees Table 6.3 months treatment groups increase by 41.07 yuan/head/sky of net income than the voluminous milk of matched group 10.53kg/ head/sky after the drug withdrawal.About 620 yuan of the medical expense of matched group 2 cow head generation mastitiss.Have 5 hairs to give birth to clinical mastitis in the matched group, treatment cost is lost above 1000 yuan.
Table 6 Economic and Efficiency Analysis
Figure BDA0000147757440000091
Embodiment 5: compound Chinese medicinal preparation is to the observation of curative effect of milch cow, goat acute mastitis.
1 compound Chinese medicinal preparation:
1.1 powder: embodiment 1 prescription.
1.2 decoct: embodiment 2 prescriptions.
2 experimental animals:
Be selected from and defend hilllock dairy industry company limited Chunhua, Xi Gang, 3 scale cattle farms of Tianyuan and 3 peasant household milch cow residential quarters, totally 6 pastures clinical symptoms of raising is significantly suffered from 180 of the lactating cows of mastitis.
Be selected from clinical symptoms that six directions bamboo town sheep stud raises and significantly suffer from 23 of the lactogenic goats of mastitis.
Choice criteria: breast and milk have significantly unusual, and the part swelling of breast infection is rubescent, and heat and pain are arranged, and thin slice, floccule, pus piece or blood are arranged in the milk, and milk is rare water sample or squeezes not get off.Serious breast shows unexpected swelling, heating, hardening, the breast district is responsive, in addition the performance fervescence, diet subtracts greatly or useless exhausted, spirit is tired etc.Milk production of cow descends, the suckling of lactogenic goat refusal lamb.
3 groupings and processing:
3.1 at random 180 ill milch cows are divided into 1 group of test, test 2 groups and matched group, every group is 60.Test 1 group and use powder, add an amount of warm water and stir, gavage (the N/R milch cow that searches for food is mixed allow it search for food voluntarily) with dispensing bottle in concentrate, 400g/ head/d (suitably adding and subtracting according to the milch cow body weight) is respectively 8; 00 and 16:00 submit from fear for twice, being used in conjunction 3d is a course for the treatment of.Test 2 groups and use decoct, 1 dose/d, usage is identical with 1 group.The matched group medication: light disease is used penicillin 300~8,000,000 units, streptomycin 3~5g, and intramuscular injection, every day 2 times, being used in conjunction 3 days is a course for the treatment of; Serious symptom adds the liquid angular vein with penicillin 8,000,000 units and instils, and is used in conjunction 3 days every day 1 time, changes intramuscular injection after the remission, is used in conjunction 3 days, and the streptomycin usage is with light disease.3 groups of cases are tight body temperature and the local lump situation that disappears of observing after medication all.
3.2 23 ill milk goats are divided into 2 groups, and test is organized 12,11 of matched groups.Test group powder adds an amount of warm water and stirs, and gavages (the N/R goat that searches for food is mixed allow it search for food voluntarily) with dispensing bottle in concentrate, and 80g/ head/d is respectively 8; 00 and 16:00 submit from fear for twice, being used in conjunction 3d is a course for the treatment of.Matched group penicillin 80~2,400,000 units, streptomycin 1~2g, intramuscular injection, every day 2 times, being used in conjunction 3 days is a course for the treatment of.
4 therapeutic effect criterion:
Judge after treating 1~2 course for the treatment of, mainly according to clinical symptoms.
Cure: the breast district is red, swollen, hot, pain disappears, and the normal and milk yield recovery of milk perusal is spiritual, appetite is normal.
Effectively: the breast district is red, swollen, hot, pain obviously alleviates, and the milk floccule disappears substantially or obviously reduces, and milk yield is gone up.
Invalid: after two courses for the treatment of, breast district is red, swollen, hot, pain does not have and obviously alleviates, and milk is still rare clearly or turn to be yellow, and flocculus is still more obvious, or the course of disease does not subtract and uses other method therapist instead in therapeutic process.
5 results and analysis:
As can be seen from Table 7, Chinese powder medicine treatment mammitis of cow, cure rate reaches 90%, and higher 1.67 percentage points than decoct treatment mammitis of cow cure rate, two groups therapeutic effect is close, no difference of science of statistics.Penicillin and streptomycin treatment milch cow Mammitis of cattle effect is lower than compound Chinese medicinal preparation, and reason is that the pathogenic bacterium that cause the generation of mastitis have produced drug resistance to penicillin and streptomycin.Effect did not show the author after more number mainly referred to a course of therapy in the table, in order not cause economic loss, used other antibiotic therapies instead.
The therapeutic effect contrast of table 7 milch cow test group and matched group
Group A treatment number The number of fully recovering for 1 course for the treatment of The number of fully recovering for 2 courses for the treatment of Cure number More do not count Cure rate (%)
Test 1 60 49 5 54 6 90
Test 2 60 48 5 53 7 88.33
Contrast 60 41 8 49 11 81.67
The therapeutic effect contrast of table 8 milk goat test group and matched group
Group A treatment number The number of fully recovering for 1 course for the treatment of The number of fully recovering for 2 courses for the treatment of Cure number More do not count Cure rate (%)
Test 1 12 8 3 11 1 91.67
Contrast 11 5 4 9 2 81.82
As shown in Table 8, lactogenic goat mastitis is better than antibiotic therapy with the treatment by Chinese herbs treatment.In process of the test, lactogenic goat of matched group uses antibiotic therapy after 2 days, and breast is red, swollen, hot, the pain transference cure is not obvious, use ciprofloxacin injection treatment instead after 2 days, also do not feel any better, gavage one day Chinese powder medicine after, the breast deliquescing, transference cure after the course for the treatment of.
6 brief summaries and discussion:
Antibiotic is listed in the choice drug for the treatment of acute mastitis in decades all over the world, owing to prolonged and repeated use, made pathogenic microorganism produce Drug resistance, and reduced curative effect, and the Ruzhong residues of antibiotics is increased.Directly endangering human beings'health.And the employing treatment by Chinese herbs, concerning most Chinese herbal medicine, it is residual not contain medicine, as long as reasonable recipe, uses properly, uses in addition and makes things convenient for, and just can obtain same curative effect.Treatment by Chinese herbs milch cow, goat mastitis are from all, not only take stopgap measures but also effect a permanent cure, and promote the raising of breast autoimmunity and milk yield simultaneously.The animal and veterinary worker had strengthened the research of Chinese herbal medicine aspect in recent years, had developed the prescription of many treatment mammitis of cow, replaced antibiotic treatment with the less Chinese herbal and crude drugs preparations of side effect, and its therapeutic effect is sure.

Claims (5)

1. Chinese medicine of preventing and treating the mammals garget, it is characterized in that it is made up of the component of following parts by weight: 30~60 parts of Herba Taraxacis, 20~50 parts of SEMEN VACCARIAE, 30~60 parts of Radix Astragali, 30~60 parts of Caulis Akebiaes, 10~30 parts of Fructus Forsythiaes, 10~30 parts of Spina Gleditsiaes, 10~30 parts of Fructus Liquidambaris, 10~30 parts of Radix Bupleuri, 10~30 parts of Radix Rhapontici, 3~5 parts in Radix Glycyrrhizae;
Wherein, described mammals domestic animal is cattle or sheep.
2. the described preparation method of Chinese medicine of claim 1, it is characterized in that, Herba Taraxaci, SEMEN VACCARIAE, Radix Astragali, Caulis Akebiae, Fructus Forsythiae, Spina Gleditsiae, Fructus Liquidambaris, Radix Bupleuri, Radix Rhapontici and Radix Glycyrrhizae difference dryness in the sun with formula ratio, again respectively after oven dry under 50~60 ℃, 60 mesh sieves are crossed in all the other prescription mixing pulverizing except SEMEN VACCARIAE, pulverize after the mixing of will all filling a prescription again again, cross 100 mesh sieves, namely.
3. the described preparation method of Chinese medicine of claim 1, it is characterized in that, with Herba Taraxaci, SEMEN VACCARIAE, Radix Astragali, Caulis Akebiae, Fructus Forsythiae, Spina Gleditsiae, Fructus Liquidambaris, Radix Bupleuri, Radix Rhapontici and the Radix Glycyrrhizae dryness in the sun of formula ratio, add the water logging bubble of 10 times of weight, decoct 2 times merging filtrate, make decoct.
4. the application of the described Chinese medicine of claim 1 in preparation control mammals domestic animal acute mastitis medicine.
5. the application of the described Chinese medicine of claim 1 in preparation control mammals domestic animal latent mammitis medicine.
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Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4388327A (en) * 1981-10-30 1983-06-14 E. I. Du Pont De Nemours & Co. Method of increasing milk production of dairy cattle
GB2137087B (en) * 1983-03-18 1987-02-18 American Cyanamid Co Improving milk production
CN101869224B (en) * 2010-06-11 2012-09-26 浙江大学 Feed additive for improving lactation performance of lactating sows and preparation method thereof
CN101912468A (en) * 2010-07-29 2010-12-15 重庆市南川区金佛山中药材品种培育研究所 Chinese herbal medicament for treating mastitis
CN102151325B (en) * 2011-04-13 2012-07-04 苑学 Traditional Chinese medicine for treating breast diseases

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JP昭58-81741A 1983.05.17
JP昭59-179039A 1984.10.11

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