CN102499181A - Novel method for breeding Clanis bilineata Walkers - Google Patents
Novel method for breeding Clanis bilineata Walkers Download PDFInfo
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Abstract
The invention discloses a novel method for breeding Clanis bilineata Walkers, comprising the following steps of: (1) selecting larvae; (2) preserving the larvae; (3) warming the larvae; (4) airing and catching one couple of moths; (5) mating and separating the moths; (6) visually inspecting oviposition and carrying out microscopic examination; (7) disinfecting and degumming ovum surfaces; (8) hatching and stocking. The method provided by the invention has the advantages that: 1, the indoor larvae-preserving mortality rate of the Clanis bilineata Walkers is low; 2, the larvae are warmed by raising a temperature through an artificial procedure, the pupation rate of the Clanis bilineata Walkers is high, the eclosion and mating rate is high, the ovum laying amount is great and the hatching rate is high; and 3, the larvae are warmed by batches and Clanis bilineata Walker products are put into the market by batches, so as to be good for the stability of a market price and ensure the income of raisers.
Description
Technical field
The present invention relates to the cultured insect technical field, be specifically related to the red breeding new method of a kind of beans.
Background technology
Greenish brown hawk moth (Clanis Bilineata Walker) is that Lepidoptera Sphingidae greenish brown hawk moth belongs to insect, is distributed in provinces such as Hebei, Henan, Shandong, peace are little, Jiangsu, Hubei, Sichuan, Shaanxi.Greenish brown hawk moth comprises ovum, larva, pupa, 4 worm attitudes of adult throughout one's life.The beans pellet is that larval phase had for five ages, survived the winter with the form of mature larva, was commonly called as the beans pellet of going into hibernation to being commonly called as of killing bean hawkmoth larva.The beans pellet is the primary pest of soybean crops, also can endanger mung bean, cowpea etc.The beans pellet is got the food soybean leaves, can blade eaten to become to incise, and hairworm can eat into the light stalk with the beans strain for a long time.A beans pellet can be eaten up 2～3 strain beans leaves.
Because the red abundant nutrients of beans, there is the red custom of edible beans in the many areas of China.Especially there is more than the 300 year red history of edible beans Guanyun County, Jiangsu Province according to examining, and having developed hundreds of is the dish of major ingredient with the beans pellet, and various deep processed products such as can, quick-frozen beans pellet.Simultaneously, the Guanyun County has developed into the red distribution centre of beans of national maximum, and purchase is from Hunan, Hubei, Anhui, Henan, Hebei, even the beans on ground such as northeast are red, is sold to the counties and cities of periphery.It is reported that the broker that the Guanyun County, Lianyungang is engaged in the red marketing of beans now has people more than 400,8000 tons of annual turnovers, a year sale benefit reaches 1.8 hundred million yuan, makes more than 10,000 women realize seasonal employment.According to the statistics made by the departments concerned, the red annual turnover of Guanyun County beans was 8000 tons in 2005, and the whole county relies on the red related industry benefit of beans to reach 1.8 hundred million yuan.At the beginning of 2007 red 7 months listing just the time price per jin up to 300 yuan, at the bottom of August, the beginning of September peaks the phase, even also want one jin of seven, eight yuan this moment.At present, to the red gaps in market of fresh beans in spring, the red artificial greenhouse of beans is cultured and is begun to take shape, and area reaches more than 1000 mu.What adopt is greenhouse canopy breeding, the mode that field planting soybean and cover net are cultured, and flow process is: choose the beans pellet of going into hibernation on (1) market and plant as beans are red, imbed breeding in greenhouse by solar heat or the bow canopy soil; (2) after next spring, the greenish brown hawk moth adult flew out, self-copulation, lay eggs, hatch, the 1-2 instar larvae is won together with the beans leaf, put the breed field that soybean is arranged to prior kind in a suitable place to breed; Or the female moth that catches post-coitum is put into the breed Tanaka who is covered with fly net; (4) catch the red list marketing of of the right age beans.The breeding work of this process; Except the temperature of booth is omited high than outdoor temperature; Outside effective accumulated temperature arrives in advance; Pupate, emergence, mating, lay eggs, hatching etc. all is abiogenesis, almost do not have manual intervention, beans are red plants problems such as worm keeping lethality is high, pupation rate is low, the emergence mating rate is low, egg laying amount is few, incubation rate is low, disease is serious so exist
Summary of the invention
The present invention provides a kind of beans red breeding new method; Through the red breeding overall process of beans is carried out manual intervention; Have the red advantages such as worm keeping lethality is low, pupation rate is high, emergence mating rate height, egg laying amount is many, incubation rate is high, disease is few of planting of beans; And convenient centralized management helps the expansion of the red breed scale of beans, helps the raising of red output of beans and culture benefit.
For achieving the above object, the present invention provides a kind of beans red breeding new method, it is characterized in that, comprising:
(1) seed selection: select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.
(2) protect kind: protect kind of the indoor breeding frame of taking, plant very thin stall with goods spread out on the ground for sale and be placed in the plastic box beans are red, on cover sandy soil, plastic box is put in protects on kind of the frame then.Or directly plant very thin stall with goods spread out on the ground for sale and be placed on and protect on kind of the frame and cover sandy soil beans are red.Keep room temperature 0-10 ℃, humidity 70%-90%.
(3) warm seeds: in 2-8 month, the red kind of the beans of protecting kind of chamber moved to successively in batches that warm seeds are indoor heats up gradually, reach and keep behind the suitable temperature short its of flat temperature to pupate and sprout wings.
(4) dry in the air moth one to one: after the greenish brown hawk moth wing of emergence launches, lure it to fly on the curtain that gauze or stalk make with fluorescent lamp.Per hour inspection is once grasped the greenish brown hawk moth of mating in the mating basket gently, is transferred to copulation chamber.
(5) mating separation of copulating moth: the male and female moth is taken apart gently at the copulation chamber post-coitum.Necessary, male moth can carry out the secondary mating under cooling protection.
(6) the visual inspection microscopy of laying eggs: the female moth visual examination intersegmental membrane behind the separation of copulating moth, destroy have slag point for being with poison moth.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs, or has on the plastic film of frame around putting and lay eggs.Female moth after laying eggs is by only planting pin from the joint dorsal blood vessel with inspection; Adipose tissue in abdomen of picking, be put in grind in the 1% sodium hydroxide drop on the slide after, high-power microscope is inspection down; As find the viral inclusion body particle, then destroy together with the ovum that it produced.
(7) disinfection of egg surface is come unstuck: qualified ovum is put into solution and is rubbed rapidly, sloughs the colloid on oophyte surface, pulls the back out with 18 ℃ of clear water rinsings twice, floats the moth hair and the bad egg that float simultaneously.Then with antiseptic solution sterilization, put into 18 ℃ of clear water rinsings till do not have a medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.
(8) hatch and put in a suitable place to breed: 30cm * 20cm wooden frame, gauze is coverd with in the bottom, and two dual-purpose hinges link to each other then, make the breeding box.Sterilization and come unstuck after the red ovum of beans be put in the breeding box and hatch, temperature 15-30 ℃, relative moisture 75%-90%.Draw the red larva of beans of collection hatching just with withy, argy wormwood or cypress branch, before at 10 in the morning or after at 4 in afternoon, directly put in a suitable place to breed to leaves of plants.When outside air temperature is lower or rainy, also can be indoor or the booth heatable adobe sleeping platform in put in a suitable place to breed again behind artificial feeding's to two molting in age.
Advantage of the present invention: 1. the red indoor guarantor of beans to plant lethality low.2. manual program heats up and carries out warm seeds, and the red pupation rate of beans is high, the emergence mating rate is high, egg laying amount is many, incubation rate is high.3. warm seeds in batches, the red product of control beans goes on the market in batches, helps the stable of the market price, guarantees raiser's income.4. through visual inspection, microscopy, three steps of the disinfection of egg surface, got rid of the generation of disease during larva is cultured basically.5. except that Soybean Leaves, select for use Folium sophorae, kudzu leaf as the red feed of culturing of beans, plant is high, and the leaf amount is big.Above measure can significantly improve the red per mu yield of culturing of beans, helps enlarging the red breed scale of beans.
Embodiment
The red breeding new method of a kind of beans is characterized in that, comprising:
(1) seed selection: select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.
(2) protect kind: protect kind of the indoor breeding frame of taking, plant very thin stall with goods spread out on the ground for sale and be placed in the plastic box beans are red, on cover the 10-15cm sandy soil, plastic box is put in protects on kind of the frame then.Or directly plant very thin stall with goods spread out on the ground for sale and be placed on and protect on kind of the frame and cover the 10-15cm sandy soil beans are red.Keep room temperature 0-10 ℃, humidity 70%-90%.
(3) warm seeds: in 2-8 month, the red kind of the beans of protecting kind of chamber moved to successively in batches that warm seeds are indoor heats up gradually, every day, the temperature rising was 1-2 ℃, kept after rising to 15-30 ℃ short its of flat temperature to pupate and sprout wings.
(4) dry in the air moth one to one: after the greenish brown hawk moth wing of emergence launches, lure it to fly on the curtain that vertically suspended gauze or stalk make with black light lamp or ultraviolet light.Per hour inspection is once grasped the greenish brown hawk moth of mating in the mating basket gently, is transferred to copulation chamber.
(5) mating separation of copulating moth: 15-30 ℃ of copulation chamber temperature, ventilate dark.After mating 8-20 hour the male and female moth is taken apart gently.Necessary, male moth can be at 10 ± 1 ℃ of cooling protections, to carry out the secondary mating.
(6) the visual inspection microscopy of laying eggs: the female moth visual examination 2-6 intersegmental membrane behind the separation of copulating moth, destroy have slag point for being with poison moth.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs, or cuts off about 2/3 the wing and the digitus of 3 pairs of pereiopoda, has around putting on the plastic film of frame to lay eggs.Female moth after laying eggs is by only planting pin from 2-4 joint dorsal blood vessel with inspection; Adipose tissue in abdomen of picking, be put in grind in the 1% sodium hydroxide drop on the slide after, high-power microscope is inspection down; As find the viral inclusion body particle, then destroy together with the ovum that it produced.
(7) disinfection of egg surface is come unstuck: qualified ovum is put into the caustic soda of 1%-10% or the sodium carbonate liquor of 0.1%-2.0% was rubbed rapidly 1-10 minute; Slough the colloid on oophyte surface; Pull the back out with 15-30 ℃ of clear water rinsing twice, float the moth hair and the bad egg that float simultaneously.Use concentration to be the formalin of 1-10% sterilization 10-30 minute then, put into 15-30 ℃ of clear water rinsing till do not have a medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.
(8) hatch and put in a suitable place to breed: 30cm * 20cm wooden frame, gauze is coverd with in the bottom, and two dual-purpose hinges link to each other then, make the breeding box.Sterilization and come unstuck after the red ovum of beans be put in the breeding box and hatch, temperature 15-30 ± 1 ℃, relative moisture 75%-90%.Draw the red larva of beans of collection hatching just with withy, argy wormwood or cypress branch, before at 10 in the morning or after at 4 in afternoon, directly put in a suitable place to breed to Soybean Leaves, Folium sophorae or kudzu leaf.When outside air temperature is lower or rainy, also can be indoor or the booth heatable adobe sleeping platform in put in a suitable place to breed again behind artificial feeding's to two molting in age.
Situation such as the growth cycle of greenish brown hawk moth, characteristics under the following brief account are so that those skilled in the art better understands this technical scheme.
Greenish brown hawk moth comprises ovum, larva, pupa, 4 forms of adult throughout one's life.
1. adult: the long 40-45 millimeter of body, health and wing yellowish-brown, many fine hair.Cephalothorax dorsal line crineous, belly respectively saves back side trailing edge has the brownish black band.Fore wing is long and narrow, and there is bigger semicircle crineous in the nearly central authorities of leading edge, and there is reddish brown foxiness the base portion top, near the yellowish-brown relief angle.
2. ovum: nearly spheroidal, diameter 2-3 millimeter.Light green color during primiparity, the gradual change yellow-white darkens before the hatching.
3. larva: body is a cylindrical shape, and belly is made up of 10 joints.Pore has on preceding 8 joints.4 pairs of abdominal foots are given birth on the 3-6 joint.One caudal horn is arranged on the octoon, and length is different because of the length of time.The red larva of beans is totally 5 ages.The identification in the length of time can nose shape and the caudal horn characteristic carry out.1 age and 5 ages are rounded, and 5 age the head breadth bigger about 9 times than 1 age.2,3,4 ages triangular in shape, crown point, but width is different, 3 ages with 4 age the caudal horn shape different, the caudal horn in 4 ages is curved slightly.
Specific as follows: be the caudal horn pitchy 1 age, be about 2 millimeters, be oblique craspedodrome, body is long; In 2 ages, caudal horn is filbert, is about 3 millimeters, becomes tiltedly to keep straight on; In 3 ages, caudal horn is khaki, is about 4 millimeters, is oblique craspedodrome; In 4 ages, the caudal horn pistac is about 6 millimeters, and base portion is thick, and the end is downwarping slightly; In 5 ages, head is green, the health yellow green; The yellow granule of close life, from belly the 1st joint, there are 7 pairs of yellow-white twills the health both sides; The caudal horn yellow green is about 6 millimeters, and the caudal horn base portion is thick; The end downwarping, 1-5 average body in age length is respectively 1 centimetre, 1.4 centimetres, 2.0 centimetres, 3.6 centimetres, 6.8 centimetres.
4. pupa: the long 40-50 millimeter of body, spindle, bronzing.One band ditch is respectively arranged before the 5-7 podomere valve.Cremaster triangle, surface have many particle projections, and end is bifurcated not.
The enforcement of present technique scheme is described with specific embodiment below.
Embodiment one:
Select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.Protect kind of the indoor breeding frame of taking, plant very thin stall with goods spread out on the ground for sale and be placed in the plastic box beans are red, on cover the 10cm sandy soil, plastic box is put in protects on kind of the frame then.Keep 0 ℃ of room temperature, humidity 70%.February, to shift a collection of beans pellet and plant to warm seeds are indoor and heat up gradually, every day, temperature rose 1 ℃, kept after rising to 15 ℃ short its of flat temperature to pupate and sprout wings.The greenish brown hawk moth wing of sprouting wings lures it to fly on the vertically suspended gauze with black light lamp after launching.Per hour inspection is once grasped the greenish brown hawk moth of mating in the mating basket gently, is transferred to copulation chamber.15 ℃ of copulation chamber temperature are ventilated dark.Mating is taken the male and female moth apart after 8 hours gently.Female moth visual examination 2 intersegmental membranes behind the separation of copulating moth are destroyed the band poison moth that slag point is arranged.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs.Female moth after laying eggs by only with inspection kind of pin from 2 joint dorsal blood vessels, adipose tissue in abdomen of picking, be put in grind in the 1% sodium hydroxide drop on the slide after, high-power microscope is inspection down, destroys to have the female moth of viral inclusion body particle and the ovum that is produced thereof.Qualified ovum is put into 1% soda lye and was rubbed rapidly 3 minutes, sloughs the colloid on oophyte surface, pulls the back out with 15 ℃ of clear water rinsings twice, floats the moth hair and the bad egg that float simultaneously.Using concentration then is 3% formalin sterilization 10 minutes, puts into 15 ℃ of clear water rinsings till do not have a medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.Sterilization and come unstuck after the red ovum of beans be put in the breeding box and hatch 15 ℃ of temperature, relative moisture 75%.Draw the red larva of beans of collection hatching just with argy wormwood, before at 10 in the morning, put in a suitable place to breed to Soybean Leaves, continue to get the food growth.
Embodiment two:
Select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.Protect kind of the indoor breeding frame of taking, just the very thin stall with goods spread out on the ground for sale of the red kind of beans is placed on guarantor's kind of frame and covers the 10cm sandy soil.Keep 5 ℃ of room temperatures, humidity 80%.April, to shift a collection of beans pellet and plant to warm seeds are indoor and heat up gradually, every day, temperature rose 2 ℃, kept after rising to 25 ℃ short its of flat temperature to pupate and sprout wings.After the greenish brown hawk moth wing of sprouting wings launches, lure it to fly on the curtain that vertically suspended stalk makes with ultraviolet light.Per hour inspection is once grasped the greenish brown hawk moth of mating in the mating basket gently, is transferred to copulation chamber.25 ℃ of copulation chamber temperature are ventilated dark.Mating is taken the male and female moth apart after 10 hours gently.Male moth is at 10 ℃ of cooling protections, and is used for the secondary mating.Female moth visual examination 4 intersegmental membranes behind the separation of copulating moth are destroyed the band poison moth that slag point is arranged.The qualified female moth of visual inspection cuts off about 2/3 the wing and the digitus of 3 pairs of pereiopoda, has around putting on the plastic film of frame to lay eggs.Female moth after laying eggs by only with inspection kind of pin from 3 joint dorsal blood vessels, adipose tissue in abdomen of picking, be put in grind in the 1% sodium hydroxide drop on the slide after, high-power microscope is inspection down, destroys to have the female moth of viral inclusion body particle and the ovum that is produced thereof.Qualified ovum is put into 0.5% sodium carbonate liquor and was rubbed rapidly 3 minutes, sloughs the colloid on oophyte surface, pulls the back out with 25 ℃ of clear water rinsings twice, floats the moth hair and the bad egg that float simultaneously.Using concentration then is 3% formalin sterilization 25 minutes, puts into 25 ℃ of clear water rinsings till do not have a medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.Sterilization and come unstuck after the red ovum of beans be put in the breeding box and hatch 25 ℃ of temperature, relative moisture 80%.Draw the red larva of beans of collection hatching just with the cypress branch, in indoor or booth heatable adobe sleeping platform,, after at 4 in afternoon, directly put in a suitable place to breed and continue to get food to the kudzu leaf and grow with behind kudzu leaf artificial feeding to two molting in age.
Embodiment three
Select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.Protect kind of the indoor breeding frame of taking, plant very thin stall with goods spread out on the ground for sale and be placed in the plastic box beans are red, on cover the 15cm sandy soil, plastic box is put in protects on kind of the frame then.Keep 10 ℃ of room temperatures, humidity 90%.August, to shift a collection of beans pellet and plant to warm seeds are indoor and heat up gradually, every day, temperature rose 2 ℃, kept after rising to 30 ℃ short its of flat temperature to pupate and sprout wings.After the greenish brown hawk moth wing of sprouting wings launches, lure it to fly on the curtain that vertically suspended gauze or stalk make with black light lamp or ultraviolet light.Per hour inspection is once grasped the greenish brown hawk moth of mating in the mating basket gently, is transferred to copulation chamber.30 ℃ of copulation chamber temperature are ventilated dark.Mating is taken the male and female moth apart after 20 hours gently.Female moth visual examination 6 intersegmental membranes behind the separation of copulating moth are destroyed the band poison moth that slag point is arranged.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs.Female moth after laying eggs by only with inspection kind of pin from 4 joint dorsal blood vessels, adipose tissue in abdomen of picking, be put in grind in the 1% sodium hydroxide drop on the slide after, high-power microscope is inspection down, destroys to have the female moth of viral inclusion body particle and the ovum that is produced thereof.Qualified ovum is put into 1% caustic soda or 0.5% sodium carbonate liquor was rubbed rapidly 3 minutes, sloughs the colloid on oophyte surface, pulls the back out with 30 ℃ of clear water rinsings twice, floats the moth hair and the bad egg that float simultaneously.Using concentration then is 3% formalin sterilization 30 minutes, puts into 30 ℃ of clear water rinsings till do not have a medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.Sterilization and come unstuck after the red ovum of beans be put in the breeding box and hatch 30 ℃ of temperature, relative moisture 90%.Draw the red larva of beans of collection hatching just with withy, in the booth heatable adobe sleeping platform,, after at 4 in afternoon, put in a suitable place to breed and continue to get food to the Soybean Leaves and grow with behind Soybean Leaves artificial feeding to two molting in age.
The above; Be merely embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technical staff who is familiar with the present technique field is in the technical scope that the present invention discloses; Can expect easily changing or replacement, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of said claim.
Claims (10)
1. the red breeding new method of beans is characterized in that, comprising:
(1) seed selection: select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.
(2) protect kind: protect kind of the indoor breeding frame of taking, plant very thin stall with goods spread out on the ground for sale and be placed in the plastic box beans are red, on cover sandy soil, plastic box is put in protects on kind of the frame then.Or directly plant very thin stall with goods spread out on the ground for sale and be placed on and protect on kind of the frame and cover sandy soil beans are red.Keep room temperature 0-10 ℃, humidity 70%-90%.
(3) warm seeds: in 2-8 month, the red kind of the beans of protecting kind of chamber moved to successively in batches that warm seeds are indoor heats up gradually, reach and keep behind the suitable temperature short its of flat temperature to pupate and sprout wings.
(4) dry in the air moth one to one: after the greenish brown hawk moth wing of emergence launches, lure it to fly on the curtain that gauze or stalk make with fluorescent lamp.Per hour inspection is once grasped the greenish brown hawk moth of mating in the mating basket gently, is transferred to copulation chamber.
(5) mating separation of copulating moth: the male and female moth is taken apart gently at the copulation chamber post-coitum.Necessary, male moth can carry out the secondary mating under cooling protection
(6) the visual inspection microscopy of laying eggs: the female moth visual examination intersegmental membrane behind the separation of copulating moth, destroy have slag point for being with poison moth.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs, or has on the plastic film of frame around putting and lay eggs.Female moth after laying eggs is by only planting pin from the joint dorsal blood vessel with inspection; Adipose tissue in abdomen of picking, be put in grind in the 1% sodium hydroxide drop on the slide after, high-power microscope is inspection down; As find the viral inclusion body particle, then destroy together with the ovum that it produced.
(7) disinfection of egg surface is come unstuck: qualified ovum is put into solution and is rubbed rapidly, sloughs the colloid on oophyte surface, pulls the back out with 18 ℃ of clear water rinsings twice, floats the moth hair and the bad egg that float simultaneously.Then with antiseptic solution sterilization, put into 18 ℃ of clear water rinsings till do not have a medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.
(8) hatch and put in a suitable place to breed: 30cm * 20cm wooden frame, gauze is coverd with in the bottom, and two dual-purpose hinges link to each other then, make the breeding box.Sterilization and come unstuck after the red ovum of beans be put in the breeding box and hatch, temperature 15-30 ℃, relative moisture 75%-90%.Draw the red larva of beans of collection hatching just with withy, argy wormwood or cypress branch, before at 10 in the morning or after at 4 in afternoon, directly put in a suitable place to breed to leaves of plants.When outside air temperature is lower or rainy, also can be indoor or the booth heatable adobe sleeping platform in put in a suitable place to breed again behind artificial feeding's to two molting in age.
2. the red breeding new method of a kind of beans according to claim 1 is characterized in that, in the said step (2), plant very thin stall with goods spread out on the ground for sale and be placed in the plastic box beans are red, on cover the 10cm-15cm sandy soil.
3. the red cultural method of a kind of beans according to claim 1; It is characterized in that, in the said step (3), the red kind of the beans of protecting kind of chamber moved to the indoor intensification gradually of warm seeds successively in batches; Every day, the temperature rising was 1-2 ℃, kept after rising to 15-30 ℃ short its of flat temperature to pupate and sprout wings.
4. the red cultural method of a kind of beans according to claim 1 is characterized in that in the said step (4), fluorescent lamp is black light lamp or ultraviolet light.
5. a kind of high yield breeding method of bean hawk moth according to claim 1 is characterized in that, in the said step (5), the copulation chamber condition is temperature 15-30 ℃, ventilate, and dark, mating time is 8-20 hour.
6. the red cultural method of a kind of beans according to claim 1 is characterized in that, in the said step (6), visual inspection be that female moth 2-6 intersegmental membrane has or not the slag point.
7. the red cultural method of a kind of beans according to claim 1; It is characterized in that in the said step (6), the qualified female moth of visual inspection is directly put into kraft envelope and lays eggs; Or cut off about 2/3 the wing and the digitus of 3 pairs of pereiopoda, have around putting on the plastic film of frame and lay eggs.
8. the red breeding new method of a kind of beans according to claim 1; It is characterized in that; In the said step (6); The female moth that fat is organized as after laying eggs in the abdomen of picking is obtained from 2-4 joint dorsal blood vessel with inspection kind of pin, be put in grind in the 1% sodium hydroxide drop on the slide after, high-power microscope is checked down.
9. the red breeding new method of a kind of beans according to claim 1 is characterized in that, in the said step (7), the used solution of sterilizing is 3% formalin sterilization, and the time is 10-30 minute.
10. the red breeding new method of a kind of beans according to claim 1 is characterized in that, in the said step (8), the leaves of plants that the red growth of beans is fed is Folium sophorae or kudzu leaf.
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| CN111587846A (en) * | 2020-07-13 | 2020-08-28 | 江苏省农业科学院 | Egg laying device special for bean hawkmoth and method for improving egg laying amount of bean hawkmoth based on egg laying device |
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| CN112931504A (en) * | 2021-01-27 | 2021-06-11 | 连云港市农业科学院 | Disinfectant for bean hawkmoth eggs |
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| CN112889758A (en) * | 2021-01-27 | 2021-06-04 | 连云港市农业科学院 | Environmental control condition for artificial indoor culture of bean hawkmoth |
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