CN111587846A - Egg laying device special for bean hawkmoth and method for improving egg laying amount of bean hawkmoth based on egg laying device - Google Patents
Egg laying device special for bean hawkmoth and method for improving egg laying amount of bean hawkmoth based on egg laying device Download PDFInfo
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- CN111587846A CN111587846A CN202010667347.7A CN202010667347A CN111587846A CN 111587846 A CN111587846 A CN 111587846A CN 202010667347 A CN202010667347 A CN 202010667347A CN 111587846 A CN111587846 A CN 111587846A
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- 230000017448 oviposition Effects 0.000 title claims abstract description 99
- 241000256011 Sphingidae Species 0.000 title claims abstract description 74
- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 68
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title claims abstract description 19
- 235000013601 eggs Nutrition 0.000 claims abstract description 48
- 235000012907 honey Nutrition 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 244000068988 Glycine max Species 0.000 claims description 8
- 235000010469 Glycine max Nutrition 0.000 claims description 8
- 241000981121 Leguminivora glycinivorella Species 0.000 claims description 8
- 230000001680 brushing effect Effects 0.000 claims description 8
- 230000013011 mating Effects 0.000 claims description 8
- 239000004677 Nylon Substances 0.000 claims description 7
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- 229910001220 stainless steel Inorganic materials 0.000 claims description 6
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- 210000001015 abdomen Anatomy 0.000 claims description 4
- 238000003825 pressing Methods 0.000 claims description 3
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- 239000003365 glass fiber Substances 0.000 claims description 2
- 239000013589 supplement Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 241001586734 Clanis bilineata Species 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000003416 Asparagus officinalis Species 0.000 description 2
- 235000005340 Asparagus officinalis Nutrition 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001363650 Thysanoplusia orichalcea Species 0.000 description 2
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- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 241000254137 Cicadidae Species 0.000 description 1
- 241000272184 Falconiformes Species 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000219977 Vigna Species 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a special spawning device for a bean hawkmoth, which comprises a fixed frame and a spawning net cage, wherein the spawning net cage is fixed in the fixed frame through a connecting piece; an opening is formed in the side face or the top face of the spawning net cage, and the opening is opened and closed through a zipper; the aperture of the spawning net cage is 50-80 meshes; and a honey water box is also arranged in the spawning net cage. The invention also discloses a method for improving the egg laying amount of the bean hawkmoth based on the egg laying device, which comprises the three steps of obtaining mated female adult bean hawkmoth, creating an egg laying environment and collecting eggs. The spawning device is simple to manufacture, simple in structure, low in cost, small in occupied space, convenient to move and easy to observe the spawning condition of the female bean hawkmoth adults. The invention obviously improves the egg laying rate of the female bean hawkmoth adults and provides powerful support for large-scale industrial culture of the bean hawkmoths.
Description
Technical Field
The invention belongs to the field of insect feeding, and particularly relates to a special egg laying device for a bean hawkmoth and a method for improving the egg laying amount of the bean hawkmoth based on the special egg laying device.
Background
Bean hawkmoth (Clanis bilineata) belonging to Lepidopetera of Insecta, Sphingideae, and fed with soybean leaf, mung bean leaf, cowpea leaf and locust leaf. The larvae are commonly called bean worms, bean hawks and bean cicadas. The clanis bilineata tsingtauica is rich in 18 amino acids and 8 fatty acids, is a high-quality complete protein insect, is rich in polysaccharide substances with strong antioxidant activity, and has the effects of resisting aging and reducing blood fat. The clanis bilineata tsingtauica can be used as a safe and reliable protein source for human, meets the requirements of people on health, and has important economic value.
The adult bean hawkmoth has the body length of 40-45 mm and the wing spread of 100-200 mm, the body and the wings are yellow brown, the central line of the back of the head and the chest is dark brown, the back of each section of the back of the abdomen is dark black and constant in temperature, the front wing is long and narrow, the back wing is small, and the part near the back corner is yellow brown. Adults have the habit of going out at night in the daytime and start to move at night, and have strong tendency to black light. The female and male adults lay eggs in 3 hours after mating, each female insect lays eggs about 200-380 grains, and the service life of the adult is about 4-7 days.
The bean hawkmoth develops through four stages of egg, larva, pupa and imago, and belongs to a completely metamorphotic insect. Wherein, the key is to obtain a large batch of eggs which are well hatched. Therefore, how to create an environment suitable for the female adult soybean hornworm to lay eggs in the limited feeding space is very important for improving the egg laying rate. The traditional method is to make the soybean hornworm oviposit by using a closed oviposition box. The egg laying rate of the bean hawkmoth is low, the time and the labor are consumed for collecting the eggs, the efficiency is low, sufficient egg laying rate of the bean hawkmoth cannot be provided for the market, and the market demand cannot be met.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the technical defects existing in the artificial feeding and oviposition process of the female adult soybean pod moths, the application provides a special oviposition device for the soybean pod moths and a method for improving the oviposition amount of the soybean pod moths based on the special oviposition device.
The technical scheme is as follows: a spawning device special for a bean hawkmoth comprises a fixed frame and a spawning net cage, wherein the spawning net cage is fixed in the fixed frame through a connecting piece; an opening is formed in the side face or the top face of the spawning net cage; the aperture of the spawning net cage is 50-80 meshes; and a honey water box is also arranged in the spawning net cage.
The opening is opened and closed through a zipper.
The aperture of the spawning cylinder mould is 60 meshes. On one hand, the air circulation is convenient, and on the other hand, the dust pollution is avoided, because if the aperture is too large, the scales of the adult soybean hornworm easily fly out of the cage, so that the dust pollution is caused. The fixed frame and the spawning net cage are both rectangular, and the length, the width and the height are preferably 100cm, 50cm and 50 cm; eight corners of the spawning net cage are fixed on the eight corners of the fixed frame through connecting pieces, so that the spawning net cage is integrally fixed in the fixed frame. The fixed frame and the spawning net cage can also adopt other suitable shapes according to actual conditions, and the sizes of the fixed frame and the spawning net cage can also be properly adjusted according to the conditions.
Preferably, the honey water box is directly placed at the bottom of the spawning net cage, so that nutrition is supplemented for the spawning bean hawkmoth conveniently. Furthermore, the edge height of the honey water box is less than or equal to 5cm (preferably 3-4cm), and the depth of the honey water in the open container is more than or equal to 2 cm.
The fixed frame is made of hard materials, can be made of plastics, aluminum alloy, iron or stainless steel and the like, and is preferably made of stainless steel.
The spawning net cage is made of nylon or glass fiber, and preferably is made of nylon.
The connecting piece is the ribbon, can be nylon ribbon, stainless steel ribbon or spraying plastics stainless steel ribbon, preferred nylon ribbon.
The opening is arranged on the top surface of the spawning net cage. The opening is provided for the convenience of placing the mated female soybean looper and brushing the eggs of the soybean looper, and is preferably provided on the top surface of the spawning net cage for the convenience of handling.
The invention also discloses a method for improving the egg laying amount of the bean hawkmoth based on the egg laying device, which comprises the following steps:
(1) obtaining mated female bean hawkmoth adults: selecting male bean hawkmoth adults and female bean hawkmoth adults to mate and then split, and selecting robust female hawkmoths with complete wings and regular scale hair;
(2) constructing a spawning environment: placing an oviposition device in an oviposition chamber, opening an oviposition net cage opening, placing 5-15% honey water in a honey water box, and placing 300-450 mated female croakers in the oviposition net cage; adjusting the environment of the oviposition chamber as follows: the temperature is 25-30 ℃, the relative humidity is 70-80%, and the ratio of light to dark cycles is 16: 8;
(3) collecting worm eggs: sending mated female bean hawkmoth adults to an oviposition chamber for 16h to oviposit, opening an opening of the oviposition device, taking out the female bean hawkmoth adults in the oviposition device, brushing eggs attached to an oviposition cage with a brush, and finishing primary egg collection; and then putting the female adult soybean pod moths back to the oviposition device, keeping the oviposition environment, opening the oviposition device again after 16 hours, brushing eggs attached to the oviposition cage with a brush, and completing secondary egg collection.
Further, in the step (1), the mating female soybean hornworm adult obtaining step comprises:
1) mating: according to the proportion of 1.2: 1, selecting male imagoes of the bean hawkmoths emerging from the 1 st to the 2 nd days and female imagoes of the bean hawkmoths emerging from the 1 st to the 2 nd days to mate for more than 16h in a mating room;
2) splitting: pressing the back of the abdomen of the female moth with the left hand, pinching the base of the male moth wing with the right hand, and lifting the female moth towards the head to open the male moth wing, so as to select the robust female moth with complete wings and regular scale hair.
Preferably, in the step (2), the honey water concentration is preferably 10%.
In the step (2), preferably, 300 to 400 mated female soybean hornworm adults are placed in an egg-laying net cage.
Further preferably, in step (2), the preferred temperature of the oviposition chamber is 28 ℃; the relative humidity is preferably 75%.
In the step (3), the time for collecting eggs is generally selected in the daytime, and the egg granules are convenient to brush in the daytime because the female bean hawkmoth adults have weak activity in the daytime.
By the method, 4-5 ten thousand eggs can be collected by one egg-laying device in the first egg-collecting process; one egg laying device can collect 1-2 ten thousand eggs. After twice egg collection, the egg laying amount of the female asparagus caterpillars in the later period is greatly reduced, and the quality is not high, so that the subsequent egg grains are not collected any more.
Has the advantages that: the air in the spawning device is easy to circulate, and the female adult can move sufficiently; a closed spawning cage is adopted, and the spawning cage with a zipper is fixed on a fixed frame, so that the operation and the collection of egg grains are convenient; honey water is placed at the bottom in the spawning cage, so that nutrition can be easily supplemented in the nutrition supplementing stage, and spawning is facilitated; the device has the advantages of simple structure, small occupied space, low cost, convenience in assembly, simplicity in operation and space saving; the female adult soybean pod moth egg laying situation can be easily observed, the movement is convenient, the egg grains are convenient to collect, and only the brush is needed to lightly brush the egg grains attached to the surface of the egg laying cage, so that the operation is simple. The invention creates a good environment suitable for the egg laying of the bean hawkmoth and facilitates the egg laying of the female adult bean hawkmoth; for artificially feeding and breeding the bean hawkmoth, the egg laying amount of the female adult is high and stable, and the egg laying amount of each female is maintained to be about 150-200; the hatching rate of the bean hawkmoth eggs is high and reaches more than 82-95 percent. The method for producing the bean hawkmoth eggs can be adopted, 30-50 egg-laying devices can be placed in one egg-laying chamber with 30 levels, 150-300 ten thousand egg granules can be produced, and the supply of the quantity of the bean hawkmoth eggs in the market can be stabilized.
Drawings
Fig. 1 is a schematic structural diagram of the device of the present application.
Detailed Description
The present application will now be described in detail with reference to the drawings and specific examples.
Example 1
As shown in fig. 1, a spawning device special for bean hawkmoth, including length x width x height dimension for 100cm x 50cm fixed frame 1 and spawning cylinder mould 2, spawning cylinder mould 2 passes through connecting piece 3 to be fixed in fixed frame 1, it is specific, eight corners of spawning cylinder mould 2 pass through connecting piece 3 to be fixed on eight corners of fixed frame 1, make spawning cylinder mould 2 fix in fixed frame 1 as a whole, form and have inside cavity, spawning device with external isolation, form the relatively independent space of female adult spawning of bean hawkmoth. Wherein the connecting piece 3 is a nylon cable tie; the fixed frame 1 is formed by welding stainless steel materials, the spawning net cage 2 is made of nylon materials, and the aperture is 60 meshes; the top surface of the spawning net cage 2 is provided with an opening 21 which is convenient for placing mated female bean hawkmoth adults and brushing the eggs of the bean hawkmoth, and the opening and the closing are realized through a zipper; the aperture of the spawning net cage 2 is 50-80 meshes; a honey water box (not shown) is also arranged at the bottom in the spawning net cage 2, so that nutrition can be supplemented for the spawning bean hawkmoth conveniently, the edge height of the honey water box is less than or equal to 5cm (preferably 3-4cm), and the depth of the honey water in an open container is more than or equal to 2 cm.
Example 2
A method for improving the egg laying amount of the Clanis bilineata tsingtauica based on the egg laying device in the embodiment 1 comprises the following steps:
(1) obtaining mated female bean hawkmoth adults:
1) mating: according to the proportion of 1.2: 1, selecting male imagoes of the bean hawkmoths emerging from the 1 st to the 2 nd days and female imagoes of the bean hawkmoths emerging from the 1 st to the 2 nd days to mate for more than 16h in a mating room;
2) splitting: slightly pressing the back of the abdomen of the female moth with the left hand, pinching the base of the male moth wing with the right hand, opening the male moth wing by lifting towards the head of the female moth, and selecting the robust female moth with complete wings and regular scale hair;
(2) constructing a spawning environment: placing a spawning device into a spawning chamber, placing 30 cuboid 100cm multiplied by 50cm spawning devices in the spawning chamber with the square of 30, opening a spawning net cage, placing 10% honey water in a honey water box, and placing 400 mated female bean hawkmoth adults into the spawning net cage; adjusting the environment of the oviposition chamber as follows: temperature 28 ℃, relative humidity 75%, light-dark period ratio 16: 8, interference of artificial activities in the egg laying process of the female bean hawkmoth adults is reduced;
(3) collecting worm eggs: sending mated female bean hawkmoth adults to an oviposition chamber for 16h to oviposit, opening an opening of the oviposition device, taking out the female bean hawkmoth adults in the oviposition device, brushing eggs attached to an oviposition cage with a brush, and finishing primary egg collection; and then putting the female adult soybean pod moths back to the spawning device, supplementing honey water and keeping the spawning environment, opening the spawning device again after 16 hours, brushing the eggs attached to the spawning cage with a brush to complete secondary spawn collection. As a result: in the first egg collecting process, one egg laying device can collect 5.4 ten thousand eggs on average; and 2.0 ten thousand eggs can be collected by one egg-laying device on average after eggs are collected for the second time. After twice egg collection, the egg laying amount of the female asparagus caterpillars in the later period is greatly reduced, and the quality is not high, so that the subsequent egg grains are not collected any more. Classifying the collected eggs according to the egg laying date, and respectively sterilizing.
Example 3
200, 250, 300, 350, 400, 450, 500 mated female soybean moths were placed in egg-laying cages according to the procedure described in example 2, respectively. Two days of egg collection were continued and the statistics are given in the table below, from which it can be seen that the total number of eggs increased first and then decreased. In order to maximize the utilization rate of the spawning cage and maximize the egg laying amount, 400 female bean hawkmoths in the spawning cage are selected.
Claims (10)
1. The egg laying device special for the bean hawkmoth is characterized by comprising a fixed frame and an egg laying net cage, wherein the egg laying net cage is fixed in the fixed frame through a connecting piece; an opening is formed in the side face or the top face of the spawning net cage; the aperture of the spawning net cage is 50-80 meshes; and a honey water box is also arranged in the spawning net cage.
2. The egg laying device special for the bean hawkmoth according to claim 1, wherein the opening is opened and closed through a zipper, and the aperture of the egg laying cylinder mould is 60 meshes.
3. The egg laying device special for the bean hawkmoth according to claim 1, wherein the fixed frame and the egg laying cage are rectangular, and the length, width and height dimensions are preferably 100cm, 50cm and 50 cm.
4. The egg laying device special for the bean hawkmoth according to claim 1, wherein the honey water box is placed at the bottom in the egg laying net cage so as to supplement nutrition for the egg laying bean hawkmoth; the edge height of the honey water box is less than or equal to 5cm, and the honey water depth is more than or equal to 2 cm.
5. The egg laying device special for the bean hawkmoth according to claim 1, wherein the fixed frame is made of stainless steel, and the egg laying net cage is made of nylon or glass fiber.
6. The egg laying device special for the bean hawkmoth according to claim 1, wherein the opening is arranged on the top surface of the egg laying cylinder.
7. The method for increasing the egg laying amount of the bean hawkmoth based on the egg laying device of any one of claims 1 to 6, comprising the steps of:
(1) obtaining mated female bean hawkmoth adults: selecting male bean hawkmoth adults and female bean hawkmoth adults to mate and then split, and selecting robust female hawkmoths with complete wings and regular scale hair;
(2) constructing a spawning environment: placing an oviposition device in an oviposition chamber, opening an oviposition net cage opening, placing 5-15% honey water in a honey water box, and placing 300-450 mated female croakers in the oviposition net cage; adjusting the environment of the oviposition chamber as follows: the temperature is 25-30 ℃, the relative humidity is 70-80%, and the ratio of light to dark cycles is 16: 8;
(3) collecting worm eggs: sending mated female bean hawkmoth adults to an oviposition chamber for 16h to oviposit, opening an opening of the oviposition device, taking out the female bean hawkmoth adults in the oviposition device, brushing eggs attached to an oviposition cage with a brush, and finishing primary egg collection; and then putting the female adult soybean pod moths back to the oviposition device, keeping the oviposition environment, opening the oviposition device again after 16 hours, brushing eggs attached to the oviposition cage with a brush, and completing secondary egg collection.
8. The method for increasing the egg laying amount of soybean hornworm according to claim 7, wherein in the step (1), the step of obtaining the mated female soybean hornworm comprises:
1) mating: according to the proportion of 1.2: 1, selecting male imagoes of the bean hawkmoths emerging from the 1 st to the 2 nd days and female imagoes of the bean hawkmoths emerging from the 1 st to the 2 nd days to mate for more than 16h in a mating room;
2) splitting: pressing the back of the abdomen of the female moth with the left hand, pinching the base of the male moth wing with the right hand, and lifting the female moth towards the head to open the male moth wing, so as to select the robust female moth with complete wings and regular scale hair.
9. The method for increasing the egg laying amount of the bean hawkmoth according to claim 7, wherein in the step (2), the honey water concentration is preferably 10%; placing 300-400 mated female bean hawkmoth adults in an egg-laying net cage; the preferred temperature of the oviposition chamber is 28 ℃; the relative humidity is preferably 75%.
10. The method for increasing the egg laying amount of the bean hawkmoth according to claim 7, wherein in the first egg collecting process, 4 to 5 ten thousand eggs can be collected by one egg laying device; one egg laying device can collect 1-2 ten thousand eggs.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112889758A (en) * | 2021-01-27 | 2021-06-04 | 连云港市农业科学院 | Environmental control condition for artificial indoor culture of bean hawkmoth |
CN112931419A (en) * | 2021-03-09 | 2021-06-11 | 扬州大学 | Method for in vitro breeding of soybean hornworm by using soybean leaves |
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2020
- 2020-07-13 CN CN202010667347.7A patent/CN111587846A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112889758A (en) * | 2021-01-27 | 2021-06-04 | 连云港市农业科学院 | Environmental control condition for artificial indoor culture of bean hawkmoth |
CN112931419A (en) * | 2021-03-09 | 2021-06-11 | 扬州大学 | Method for in vitro breeding of soybean hornworm by using soybean leaves |
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