CN102352286B - Preparation method of holozoic sea cucumber biological wine - Google Patents
Preparation method of holozoic sea cucumber biological wine Download PDFInfo
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- CN102352286B CN102352286B CN201110246904A CN201110246904A CN102352286B CN 102352286 B CN102352286 B CN 102352286B CN 201110246904 A CN201110246904 A CN 201110246904A CN 201110246904 A CN201110246904 A CN 201110246904A CN 102352286 B CN102352286 B CN 102352286B
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Abstract
The invention discloses a preparation method of holozoic sea cucumber biological wine, characterized in that the method comprises the steps of: firstly soaking dry sea cucumber in warm water, filtering the sea cucumber, and leaching the aqueous solution to obtain sea cucumber soaking solution; cutting the soaked sea cucumber along the abdomen, clearing the internal organs and the limestone, flushing the sea cucumber through running water, and crushing the sea cucumber to obtain sea cucumber powder; and then, adding the sea cucumber powder in the sea cucumber soaking solution, adding neutral protease, stirring, regulating the pH to 7-8, heating to 45-48 DEG C, hydrolyzing at a constant temperature for 12-18 h, filtering, heating the filtrate to 80-90 DEG C, deactivating enzyme for 10-15 min, cooling, adding sugar substrate, common fermenting for 2-16 weeks, modulating through Chinese medicinal herb leaking solution and filtering to obtain the finished product. The prepared wine contains sea cucumber characteristic ingredients such as sea cucumber polysaccharide sulfate, sea cucumber saponin, sea cucumber amino acid and sea cucumber collagen peptide, and the wine has the functions of anti-tumor, immunity improvement, anti-coagulation, anti- thrombus, anti-radiation, anti-virus, liver protection and blood vessel protection.
Description
Technical field
The present invention relates to a kind of beverage article, particularly relate to the preparation method of the biological wine of a kind of full nutritious sea cucumber.
Background technology
Sea cucumber is exactly protective foods and nutritive food since ancient times, has the characteristics of protein-high, lower fat, low sugar, and is rich in amino acid, VITAMINs, indispensable fatty acid and constant and the trace element of various needed by human.In the sea cucumber dry product, contain high reactivity compositions such as abundant collagen protein, protein peptide, sea cucumber saponin, sea cucumber polysaccharide.Polysaccharide is a sea cucumber body wall important component; Mainly contain two kinds: a kind of is glycosaminoglycan extracted from sea cucumber or mucopolysaccharide, is branch's mixed polysaccharide of being made up of D-N-acetylamino galactosamine, D-glucuronic acid and L-Fucose, and another kind is the sea cucumber fucoidin; It is the even polysaccharide of straight chain that constitutes by the L-Fucose; Though both composition glycosyls are different, part of hydroxyl generation sulphating is all arranged on their sugar chains, and the sulfate group polysaccharide account for sea cucumber polysaccharide 60-70%; Have antitumor, improve functions such as immunizing power, anticoagulation, induced platelet aggregation, promotion fibrinolysis, antithrombotic, anti-inflammatory, radioprotective; Have the effect that suppresses hiv virus HIV, hepatitis B viruses (HBV), simple scar exanthema virus HSV, ability liver protecting, protection vascular injury function are a kind of multifunction activity compositions; Holothurin is also claimed the sea cucumber saponin, but anticancer is synthetic, has the body immunity of raising and anticancer germ-resistant effect, also apoplexy, spastic paralysis is had certain curative effect.The holothurian collagen peptide can improve immunity functions such as scavenger cell, lymph corpuscle, plasma cell and Tegeline, resistance of human body is improved, but anticancer shifts treatment of arthritis etc.; Sea cucumber is amino acid contained abundant; Have 18 kinds; Wherein glutamic is the highest; Aspartic acid takes second place, and contains abundant l-arginine, and l-arginine has vital role in the various physiological processs such as immunizing power, cell fission, wound recovery, hormone secretion, vascular tone, insulin sensitivity and endothelial function of enhancing body; The lipid acid kind is many in the sea cucumber, and content is high, has vital role for the function of enhancing immunity and human brain etc.; 8 kinds of VITAMINss are arranged in the sea cucumber,, keep healthyly, very important effect is arranged for growing of the metabolism that promotes cell, health.
In 5,000 years historical long rivers of the Chinese nation, wine and drinks culture are always in occupation of critical role, and wine is a kind of special food, but wine is melted into again among people's the cultural life.Spirits culture has its unique status as a kind of special form of culture in traditional Chinese culture.In civilized history in several thousand, wine almost is penetrated into the every field in the social life, yet; In recent years, liquor is becoming increasingly acute with healthy contradiction, and traditional liquor is losing increasing potential consumer; The understanding of " the many drinks of liquor are harmful to health " is affecting increasing people; New growth point and breach are sought in order to extricate oneself from a predicament by liquor enterprise, get into the health promoting wine industry one after another; Hope in the health promoting wine industry, to find very important one seat, also therefore activated health promoting wine market.Sea cucumber full-nutrients biological wine is described as the title of " polyanion food-drink ", and is nutritious, functional strong, but at present, also do not have a wine can keep complete nutrition ingredients and the multifunctionality of sea cucumber on the existing market.
Summary of the invention
The purpose of this invention is to provide the preparation method of the biological wine of a kind of full nutritious sea cucumber, it can remedy the above-mentioned deficiency of existing market.
The preparation method of the biological wine of a kind of full nutritious sea cucumber is characterized in that earlier dry Stichopus japonicus leaching sea cucumber with 25-80 ℃ of immersion, with aqueous solution suction filtration, obtains the sea cucumber soak solution; The sea cucumber of soaking is cut open along belly, removed internal organ and ls, rinse well, pulverize and obtain the broken end of sea cucumber with tap water; Then the broken end of sea cucumber is joined in the sea cucumber soak solution, add neutral protease, stir, regulate PH to 7-8; Be warming up to 45-48 ℃, constant temperature hydrolysis 12-18 hour is filtered, and makes gained filtrating be warming up to 80-90 ℃; Went out enzyme 10-15 minute, cooling adds sugared substrate again, conventional fermentation 2-16 week; With the modulation of herbal medicine water extract, filter then, promptly obtain the biological wine of full nutritious sea cucumber of the present invention.
The biological wine of full nutritious sea cucumber of the present invention; Be rich in the trace element of water-soluble sea cucumber sulfated polysaccharide, collagen peptide, sea cucumber saponin, extraordinary amino acid and needed by human that human body be prone to absorb, have liver protecting, anticancer, improve body immunity, protection vascular endothelial cell, remove effects such as interior free yl.It is amber that this nutriment wine is, the mouthfeel sweet-smelling, color and luster is penetrating, nutrition is unique, functional strong, security property is high, be a kind of drink also be a kind of tonic.
Embodiment
When the biological wine of full nutritious sea cucumber of the present invention prepares; Be earlier dry Stichopus japonicus to be steeped with 25-80 ℃ of water logging; Water-soluble sulfated polysaccharide polysaccharide, saponin, VITAMINs, trace element, amino acid, protein and lipid acid in the sea cucumber are dissolved in the water; Leach sea cucumber,, obtain the sea cucumber soak solution aqueous solution suction filtration; The sea cucumber of soaking is cut open along belly, removed internal organ and ls, rinse well, pulverize and obtain the broken end of sea cucumber with tap water; Then the broken end of sea cucumber is joined in the sea cucumber soak solution, add neutral protease, stir, regulate PH to 7-8; Be warming up to 45-48 ℃, constant temperature hydrolysis 12-18 hour is degraded into micromolecular collagen peptide or amino acid with macromolecular collagen protein, is beneficial to absorption of human body; Filter then, make gained filtrating be warming up to 80-90 ℃, went out protease activity 10-15 minute; Cooling adds sugared substrate again, conventional fermentation 2-16 week; With the modulation of herbal medicine water extract, filter then, promptly obtain the biological wine of full nutritious sea cucumber of the present invention.
The biological wine alcoholic strength of above-mentioned full nutritious sea cucumber is 0.5-35 ℃; The concentration of sea cucumber sulfated polysaccharide is 0.01-50mg/kg; The concentration of sea cucumber saponin is 0.05-100mg/kg, and the mass percent of holothurian collagen peptide is 0.01-3.5%, and sea cucumber amino acid masses percentage ratio is 0.01-2.5%.
During concrete operations, take by weighing 10 kilograms of dry Stichopus japonicuss earlier, add 50 ℃ of 400 kg of water, soaked 12 hours, leach sea cucumber,, obtain 340 kilograms of sea cucumber soak solutions aqueous solution suction filtration; The sea cucumber of soaking is cut open along belly, removed internal organ and ls, rinse well with tap water; Pulverizing obtains the broken end of sea cucumber, joins then in above-mentioned 340 kilograms of sea cucumber soak solutions, adds 2 kilograms of bromelines; Stir, regulate PH=7, be warming up to 45 ℃ of constant temperature hydrolysis 12 hours; Filter, filtrating is warming up to 80-90 ℃, and enzyme 10 minutes goes out; Cooling adds sugared substrate again, conventional 8 weeks of fermentation; With herbal medicine water extract modulation, filter then, the concentration that promptly obtains the spirituosity degree and be 18 ℃, the concentration of sea cucumber sulfated polysaccharide and be 12.7mg/kg, sea cucumber saponin be the mass percent of 32.1mg/kg, collagen peptide be 0.32% and the amino acid whose mass percent of sea cucumber be 300 kilograms of 0.25% the biological wine of full nutritious sea cucumber.
Described bromeline can be used papoid, trypsinase, stomach en-instead; Described herbal medicine water extract is made up of matrimony vine, Chinese yam, Radix Glycyrrhizae, Chinese cassia tree, date, mulberries, the root of kudzu vine and water; Their mass percent scope is respectively 7-9,4-6,1-3,3-5,6-8,7-9,1-3 and 51-77, and used each component of herbal medicine water extract of present embodiment is respectively 8,5,2,4,7,8,2 and 64; Described water logging bubble is that water-soluble active ingredient sea cucumber sulfated polysaccharide, sea cucumber saponin, sea cucumber amino acid, VITAMINs and mineral substance in the sea cucumber are dipped in the solution; Described enzymolysis is collagen peptide or the amino acid that water-insoluble macromole holothurian collagen is degraded into soluble small molecular; Described sea cucumber is Japanese stichopus japonicus, Hokkaido stichopus japonicus, Northwest ginseng, Dalian stichopus japonicus, Russian red ginseng, smoked jujube ginseng, white anus Haiti melon, Black Sea ginseng, white abdomen sea cucumber, Red sea ginseng or little Thelenota ananas (Jaeger).
Full nutritious sea cucumber wine of the present invention contains sea cucumber characteristic component sea cucumber sulfated polysaccharide, sea cucumber saponin, sea cucumber amino acid, holothurian collagen peptide and mineral substance.Have antitumor, improve immunizing power, anticoagulation, induced platelet aggregation, promotion fibrinolysis, antithrombotic, anti-inflammatory, radioprotective, inhibition virus, liver protecting and protection vascular injury function.
Claims (3)
1. the preparation method of the biological wine of full nutritious sea cucumber is characterized in that earlier dry Stichopus japonicus being used warm water soaking, leaches sea cucumber, with aqueous solution suction filtration, obtains the sea cucumber soak solution; The sea cucumber of soaking is cut open along belly, removed internal organ and ls, rinse well, pulverize and obtain the broken end of sea cucumber with tap water; Then the broken end of sea cucumber is joined in the sea cucumber soak solution, add neutral protease, stir, regulate PH to 7-8; Be warming up to 45-48 ℃, constant temperature hydrolysis 12-18 hour is filtered, and makes gained filtrating be warming up to 80-90 ℃; Went out enzyme 10-15 minute, cooling adds sugared substrate again, conventional fermentation 2-16 week; With the modulation of herbal medicine water extract, filter then, promptly obtain the biological wine of full nutritious sea cucumber; Wherein said neutral protease is bromeline, papoid, trypsinase or stomach en-; Described herbal medicine water extract is made up of matrimony vine, Chinese yam, Radix Glycyrrhizae, Chinese cassia tree, date, mulberries, the root of kudzu vine and water, and their mass percent scope is respectively 7-9,4-6,1-3,3-5,6-8,7-9,1-3 and 51-77.
2. the preparation method of the biological wine of full nutritious sea cucumber as claimed in claim 1 is characterized in that described sea cucumber full-nutrients biological wine contains sea cucumber characteristic component sea cucumber sulfated polysaccharide, sea cucumber saponin, sea cucumber amino acid, holothurian collagen peptide and mineral substance; The biological wine alcoholic strength of described full nutritious sea cucumber is 0.5-35 ℃; Described sea cucumber sulfated polysaccharide concentration is 0.01-50mg/kg; Described sea cucumber saponin concentration is 0.05-100mg/kg; Described holothurian collagen peptide mass percent is 0.01-3.5%; Described sea cucumber amino acid masses percentage ratio is 0.01-2.5%.
3. the preparation method of the biological wine of full nutritious sea cucumber as claimed in claim 1 is characterized in that described sea cucumber is Japanese stichopus japonicus, Hokkaido stichopus japonicus, Northwest ginseng, Dalian stichopus japonicus, Russian red ginseng, smoked jujube ginseng, white anus Haiti melon, Black Sea ginseng, white abdomen sea cucumber, Red sea ginseng or little Thelenota ananas (Jaeger).
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| CN201110246904A CN102352286B (en) | 2011-08-26 | 2011-08-26 | Preparation method of holozoic sea cucumber biological wine |
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| CN201110246904A CN102352286B (en) | 2011-08-26 | 2011-08-26 | Preparation method of holozoic sea cucumber biological wine |
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Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103805418A (en) * | 2013-10-28 | 2014-05-21 | 山东常生源食品科技有限公司 | Sea cucumber wine |
| CN104109614A (en) * | 2014-07-08 | 2014-10-22 | 青岛浩然生态海洋水产科技有限公司 | Production method of sea cucumber wine |
| CN104435359B (en) * | 2014-12-09 | 2019-03-08 | 山东好当家海洋发展股份有限公司 | A kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid and preparation method thereof |
| CN104893928A (en) * | 2015-06-30 | 2015-09-09 | 青岛浩大海洋保健食品有限公司 | Bushen Zhuangyang Baobei compound wine |
| CN104928136A (en) * | 2015-06-30 | 2015-09-23 | 青岛浩大海洋保健食品有限公司 | Baobei compound wine with blood lipid regulating function |
| CN104928137A (en) * | 2015-06-30 | 2015-09-23 | 青岛浩大海洋保健食品有限公司 | Sea cucumber and scallop compound wine |
| CN105039094A (en) * | 2015-06-30 | 2015-11-11 | 青岛浩大海洋保健食品有限公司 | Sea cucumber-scallop composite compound wine having blood-fat regulating function |
| CN104893935A (en) * | 2015-06-30 | 2015-09-09 | 青岛浩大海洋保健食品有限公司 | Abalone and sea cucumber compound wine with effect of delaying senescence |
| CN104946491A (en) * | 2015-06-30 | 2015-09-30 | 青岛浩大海洋保健食品有限公司 | Abalone-holothurian compound wine with blood sugar reducing function |
| CN104928135A (en) * | 2015-06-30 | 2015-09-23 | 青岛浩大海洋保健食品有限公司 | Abalone and sea cucumber compound prepared wine |
| CN107334097B (en) * | 2017-08-04 | 2020-09-08 | 青岛颐圣康和生物制药有限公司 | Preparation method and application of sea cucumber extract |
| CN107744587A (en) * | 2017-09-13 | 2018-03-02 | 山东圣洲海洋生物科技股份有限公司 | A kind of sea cucumber wine for improving cardiovascular function and preparation method thereof |
| CN107418852A (en) * | 2017-09-25 | 2017-12-01 | 五宴尊酒业股份有限公司 | A kind of preparation method of strengthen immunity new functional liquor |
| CN107647325A (en) * | 2017-11-20 | 2018-02-02 | 黑龙江美丽石岛科技开发有限公司 | A kind of processing method that instant holothurian is produced using unsalted dried sea cucumbers |
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| CN1458263A (en) * | 2002-05-15 | 2003-11-26 | 谭攸恒 | Sea cucumber nutritive wine and its producing method and use |
| CN101082020A (en) * | 2006-05-31 | 2007-12-05 | 程玉山 | Production method for ocean biology nutrition wine |
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