CN104435359B - A kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid and preparation method thereof - Google Patents
A kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid and preparation method thereof Download PDFInfo
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- CN104435359B CN104435359B CN201410744941.6A CN201410744941A CN104435359B CN 104435359 B CN104435359 B CN 104435359B CN 201410744941 A CN201410744941 A CN 201410744941A CN 104435359 B CN104435359 B CN 104435359B
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- 241000965254 Apostichopus japonicus Species 0.000 title claims abstract description 127
- 239000007788 liquid Substances 0.000 title claims abstract description 110
- 239000003814 drug Substances 0.000 title claims abstract description 99
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- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 56
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000002994 raw material Substances 0.000 claims abstract description 44
- 239000000284 extract Substances 0.000 claims abstract description 41
- 239000000758 substrate Substances 0.000 claims abstract description 30
- 235000019441 ethanol Nutrition 0.000 claims abstract description 28
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- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/616—Echinodermata, e.g. starfish, sea cucumbers or sea urchins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/287—Chrysanthemum, e.g. daisy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
It is that control enzyme hydrolysis is successively carried out using compound protease, flavor protease by stichopus japonicus body wall after hot water blanching, homogenate, stichopus japonicus enzymolysis liquid is made after cooling in enzyme deactivation the present invention relates to a kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid and preparation method thereof；Schisandra chinensis, fructus lycii, wild chrysanthemum compatibility are chosen into substrate raw material, is removed and is precipitated with ethyl alcohol after obtaining Aqueous extracts with hot water decocting method, take supernatant that ethyl alcohol is evaporated off, pure water is added and deploys obtained Chinese medicine extract；Stichopus japonicus enzymolysis liquid is mixed with Chinese medicine extract, is deployed, and is concentrated, and centrifugation takes supernatant, is added auxiliary material, is stirred evenly, and successively through UHT sterilization, hot filling, re-pasteurization, protect liver stichopus japonicus traditional Chinese medicine oral liquid is made.Its protect liver stichopus japonicus traditional Chinese medicine oral liquid prescription science, sweet and sour taste have effects that dispersing stagnated hepatoqi, clearing heat and detoxicating, calming the liver are calmed the nerves, have the double action of health care and nutrition.Preparation method rational technology, operation is feasible, is suitble to industrialized production.
Description
Technical field
The present invention relates to a kind of pharmaceutical preparation for not determining structure of traditional herbal medicines, especially a kind of protect liver stichopus japonicus traditional Chinese medicine mouths
Take liquid and preparation method thereof.
Background technique
With the development of society, improvement of living standard, the crowd for facing hepar damnification is more and more huger.Excessive consumption of alcohol,
Drug abuse, diet is greasy, is all the inducement for causing hepar damnification.How liver protecting becomes urgently to be resolved at present ask
Topic.
Sea cucumber also known as stichopus japonicus, extra large mouse, sea gherkin etc. are a kind of rare aquatic/marine animals, because of its benefiting action, similar to people
Join and gains the name.Sea cucumber is not only precious food and rare medicinal material.According to being recorded in supplementary Amplifications of the Compendium of Materia Medica: sea cucumber, it is sweet in flavor
Salty, kidney tonifying, benefiting quintessence takes the photograph urine, and impotence, warm-natured benefit, sufficient enemy's ginseng, therefore named sea cucumber are treated in establishing-Yang.Sea cucumber said herein just refers to
Stichopus japonicus.It is verified through research: rich in various actives objects such as saponin(e, mucopolysaccharide, gangliosides, sterol, collagens in stichopus japonicus body
Matter, in terms of making it have various health-care effects, including liver protecting.A large number of studies show that the fucosan sulphur in stichopus japonicus
Acid esters (SC-FUC) is to the mouse liver function damage effect of being significantly improved caused by long-term alcohol；Cerebroside (SCC) is to tetrachloro
Change carbon (CCl4) cause the acute liver injury of rats effect of being significantly improved；Acidic mucopolysaccharide (SJAMP) is to diethylnitrosamine
(DEN) induced rat genesis of HCC has inhibition to make.In addition, stichopus japonicus can also significantly adjust the blood lipid level of body, effectively adjust high
Cholesterolemia lipid metabolism in rats.As it can be seen that magical effect of the stichopus japonicus in terms of protecting liver, is constantly found.And
And stichopus japonicus also has good trophism, is rich in 18 kinds of amino acid, a variety of macroelements and microelement needed by human, with
And multivitamin.
And prepare drug using stichopus japonicus for raw material and reported in open file, for example, disclosed number of patent application are as follows:
200810238717.4 Chinese invention patent application discloses " a kind of Apostichopus japonicus nourishing granules, capsule and Apostichopus japonicus nourishing granules
Processing method ", be using fresh stichopus japonicus be primary raw material, by enzymatic hydrolysis obtain be rich in protein, peptides, amino acid and soap
The enzymolysis liquid of glucoside and stichopus japonicus mucopolysaccharide isoreactivity ingredient, it is dry after cyclodextrin bioencapsulation technical treatment, then with wilsonii, Huang
The mixing of the Chinese medical extracts such as stilbene, Radix Glycyrrhizae is made after the working processes such as pelletized, dried, and has that nutritious tonifying, anti-ageing liter is white, mentions
The health cares such as high immunity and therapeutic effect.
Currently, not finding the report for preparing liver-care preparations for raw material and other Chinese medicine collocation using stichopus japonicus also.
Summary of the invention
The object of the present invention is to provide a kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid and preparation method thereof, the protect liver stichopus japonicus traditional Chinese medicine mouths
Liquid prescription science is taken, protecting and nourishing liver effect is good, non-evident effect；Preparation method rational technology, operation is feasible, is suitble to industry
Metaplasia produces.
The technical solution adopted by the present invention to solve the technical problems are as follows: a kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid, feature
Be: it includes stichopus japonicus enzymolysis liquid, Chinese medicine extract and auxiliary material, wherein the weight percent of each component is respectively as follows: stichopus japonicus enzymatic hydrolysis
Liquid 50~65%, Chinese medicine extract 25~45% and auxiliary material 5~10%；The stichopus japonicus enzymolysis liquid is to float stichopus japonicus body wall through hot water
It scalds, after homogenate, control enzyme hydrolysis is successively carried out using compound protease, flavor protease, enzyme deactivation is made after cooling；In described
Medicament extracting solution is to choose Chinese medicine Schisandra chinensis, fructus lycii, wild chrysanthemum compatibility into substrate raw material, is extracted after obtaining Aqueous extracts using hot water
It is removed and is precipitated using ethyl alcohol, after taking supernatant to remove ethyl alcohol, added pure water adjusting solid content and be made for 8~10%；
The weight percent when Schisandra chinensis, fructus lycii, wild chrysanthemum compatibility be respectively as follows: Schisandra chinensis 30~40%, fructus lycii 30~
40% and wild chrysanthemum 20~40%.
Priority scheme of the invention: the weight percentage of each component of the protect liver stichopus japonicus traditional Chinese medicine oral liquid is respectively as follows: thorn
Join enzymolysis liquid 60%, Chinese medicine extract 33% and auxiliary material 7%.
The weight percent when Schisandra chinensis, fructus lycii, wild chrysanthemum compatibility is respectively as follows: Schisandra chinensis 35%, fructus lycii
35% and wild chrysanthemum 30%.
The auxiliary material is xylitol.
A kind of preparation method of protect liver stichopus japonicus traditional Chinese medicine oral liquid, it is characterised in that: pass through following process steps:
(1) preparation of stichopus japonicus enzymolysis liquid
Choosing fresh stichopus japonicus is raw material, and stichopus japonicus is removed internal organs, and is cleaned, and after taking stichopus japonicus body wall hot water blanching, is added
Distilled water grinds pulp using colloid mill, is deployed into the stichopus japonicus solution that protein concentration is 10~20%；Stichopus japonicus solution is heated
To 90~100 DEG C, 10~30min is kept, is subsequently cooled to 40~55 DEG C, the compound protease water of solution weight 4~7 ‰ is added
1~3h is solved, the flavor protease for then adding solution weight 4~7 ‰ hydrolyzes 1~3h；90~100 are heated to after hydrolysis
DEG C, keep 20~30min enzyme deactivation；It is rapidly cooled to 3~5 DEG C after enzyme deactivation, stichopus japonicus enzymolysis liquid is made；
(2) preparation of Chinese medicine extract
Chinese medicine Schisandra chinensis, fructus lycii and wild chrysanthemum are chosen, by weight percentage are as follows: Schisandra chinensis 30~40%, fructus lycii 30~
40% and 20~40% compatibility of wild chrysanthemum at substrate raw material；Substrate raw material is changed water using hot water decocting method to extract three times, is made respectively
Obtain Aqueous extracts；It Aqueous extracts will be merged after filtering respectively three times, and obtain amalgamation liquid；By amalgamation liquid using Rotary Evaporators in 80 DEG C
After lower concentration, 30~50 DEG C are cooled to, it is 95% ethyl alcohol that concentration is slowly added under stirring, contains ethyl alcohol in whole system
Amount reaches 70~75%, and sealing stands 24~36h, and filtering takes supernatant using Rotary Evaporators at 80 DEG C, and second is evaporated off in concentration
Alcohol, adding pure water and adjusting solid content is 8~10%, and Chinese medicine extract is made；
(3) raw material mixing is prepared with finished product
Stichopus japonicus enzymolysis liquid made from weighing step (1), Chinese medicine extract made from step (2) mix in proportion, and stirring is equal
After even, it is concentrated, centrifugation takes supernatant, auxiliary material is added, after mixing evenly, successively after UHT sterilization, hot filling, re-pasteurization,
Protect liver stichopus japonicus traditional Chinese medicine oral liquid is made；Wherein, in the protect liver stichopus japonicus traditional Chinese medicine oral liquid, the weight percent of each component is distinguished
Are as follows: stichopus japonicus enzymolysis liquid 50~65%, Chinese medicine extract 25~45% and auxiliary material 5~10%.
Described substrate raw material is changed water and extracted using hot water decocting method is to decoct substrate raw material boiling water three times three times,
8~10 times of substrate raw material weight of water is wherein added for the first time, boils extraction 2 hours, is separated by solid-liquid separation；Second of addition substrate is former
The water of 5~8 times of weight of material boils extraction 1 hour, is separated by solid-liquid separation；3~6 times of substrate raw material weight of water is added in third time, boils
It extracts 1 hour, is separated by solid-liquid separation.
Protect liver stichopus japonicus traditional Chinese medicine oral liquid of the invention includes that stichopus japonicus enzymolysis liquid, Schisandra chinensis, fructus lycii and wild chrysanthemum are produced
Chinese medicine extract and auxiliary material, wherein stichopus japonicus enzymolysis liquid is the amino acid, polypeptide, polysaccharide for producing stichopus japonicus body wall after double enzymolysis
Extract is conducive to the absorption of human body, and having reduces transaminase, improves the effect of immunity of organisms.Produce Chinese medicine extract
Chinese medicine Schisandra chinensis and wild chrysanthemum are the drugs that Ministry of Public Health's approval can be used for health food, and fructus lycii is then the food of dual-purpose of drug and food.
Wherein, the main component in Schisandra chinensis is lignanoids substance, has the function of significant protecting liver, lowering enzymes, facilitates liver cell egg
The synthesis of white matter can be substantially reduced serum transaminase, effectively repair damaged liver；The substantially reduced large dosage of fructus lycii energy is drunk
With hepatic injury caused by carbon tetrachloride (CCl4)；The aqueous extract of wild chrysanthemum has clearing heat and detoxicating, dispelling wind calming the liver, improves immunity
The effect of.The common compatibility of Chinese medicine extract that stichopus japonicus enzymatic hydrolysis is produced with three taste Chinese medicines, generates synergistic effect, have dispersing stagnated hepatoqi,
The effect of clearing heat and detoxicating, calming the liver is calmed the nerves.Through animal experiments show that, liver protection function is prominent.The protect liver stichopus japonicus extract oral
Liquid prescription science, sweet and sour taste, non-evident effect can be used for preventing the daily of fatty liver, alcoholic liver, drug induced hepatic injury etc.
It health care and solves after the meal greasy, is particularly suitable for the greasy crowd of long-term excessive consumption of alcohol, drug abuse, diet.Preparation method of the invention
Rational technology, operation is feasible, is suitble to industrialized production.
Specific embodiment
Below with reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of preparation method of protect liver stichopus japonicus traditional Chinese medicine oral liquid, by following process steps:
(1) preparation of stichopus japonicus enzymolysis liquid
Choosing fresh stichopus japonicus is raw material, and stichopus japonicus is removed internal organs, and is cleaned, after taking the 80 DEG C of hot water blanchings of stichopus japonicus body wall,
Distilled water is added, grinds pulp using colloid mill, is deployed into the stichopus japonicus solution that protein concentration is 15%；Stichopus japonicus solution is heated
To 95 DEG C, 20min is kept, is subsequently cooled to 50 DEG C, the composite protease hydrolysis 2h of solution weight 5 ‰ is added, then adds
The flavor protease of solution weight 6 ‰ hydrolyzes 2h；It is heated to 95 DEG C after hydrolysis, keeps 25min enzyme deactivation；It is cold rapidly after enzyme deactivation
But to 4 DEG C, stichopus japonicus enzymolysis liquid is made；Wherein, compound protease believes compound protease using commercially available Novi；Flavor protease is adopted
Flavor protease is believed with commercially available Novi；
(2) preparation of Chinese medicine extract
Chinese medicine Schisandra chinensis, fructus lycii and wild chrysanthemum are chosen, by weight percentage are as follows: Schisandra chinensis 35%, fructus lycii 35% and open country
30% compatibility of chrysanthemum is at substrate raw material；Substrate raw material is changed water using hot water decocting method to extract three times, wherein bottom is added for the first time
The water that 9 times of raw material weight boils extraction 2 hours, is separated by solid-liquid separation, obtains first time Aqueous extracts；Second of addition substrate raw material weight
The water of 6 times of amount boils extraction 1 hour, is separated by solid-liquid separation, obtains second of Aqueous extracts；Third time is added 4 times of substrate raw material weight
Water boils extraction 1 hour, is separated by solid-liquid separation, obtains third time Aqueous extracts；It is closed after first, second and third Aqueous extracts is filtered respectively
And obtain amalgamation liquid；After amalgamation liquid is concentrated at 80 DEG C using Rotary Evaporators, 40 DEG C are cooled to, under stirring slowly
Addition concentration is 95% ethyl alcohol, so that ethanol content in whole system is reached 72%, sealing stands 30h, and filtering takes supernatant to use
For Rotary Evaporators at 80 DEG C, ethyl alcohol is evaporated off in concentration, and adding pure water and adjusting solid content is 9%, and traditional Chinese medicine extraction is made
Liquid；
(3) raw material mixing is prepared with finished product
Stichopus japonicus enzymolysis liquid made from weighing step (1), Chinese medicine extract made from step (2) mix in proportion, and stirring is equal
After even, be concentrated, centrifugation takes supernatant, add auxiliary material xylitol, after mixing evenly, successively through UHT sterilization, hot filling, secondary kill
After bacterium, protect liver stichopus japonicus traditional Chinese medicine oral liquid is made；Wherein, in the protect liver stichopus japonicus traditional Chinese medicine oral liquid, the weight percent of each component
It is respectively as follows: stichopus japonicus enzymolysis liquid 60%, Chinese medicine extract 33% and auxiliary material 7%.
In protect liver stichopus japonicus traditional Chinese medicine oral liquid manufactured in the present embodiment, stichopus japonicus enzymolysis liquid and Schisandra chinensis with synergy,
The common compatibility of Chinese medicine extract that fructus lycii and wild chrysanthemum Chinese medicine are produced, the function calmed the nerves with dispersing stagnated hepatoqi, clearing heat and detoxicating, calming the liver
Effect, has the double action of health care and nutrition.The protect liver stichopus japonicus traditional Chinese medicine oral liquid prescription science, sweet and sour taste, protecting and nourishing liver effect
Fruit is good, has no toxic side effect, and can be used for solving greasy, the daily health carings such as prevention fatty liver, alcoholic liver, drug induced hepatic injury after the meal.It is made
Preparation Method rational technology, operation is feasible, is suitble to industrialized production.
Embodiment 2
A kind of preparation method of protect liver stichopus japonicus traditional Chinese medicine oral liquid, by following process steps:
(1) preparation of stichopus japonicus enzymolysis liquid
Choosing fresh stichopus japonicus is raw material, and stichopus japonicus is removed internal organs, and is cleaned, after taking the 90 DEG C of hot water blanchings of stichopus japonicus body wall,
Distilled water is added, grinds pulp using colloid mill, is deployed into the stichopus japonicus solution that protein concentration is 20%；Stichopus japonicus solution is heated
To 100 DEG C, 10min is kept, is subsequently cooled to 55 DEG C, the composite protease hydrolysis 3h of solution weight 4 ‰ is added, then adds
The flavor protease of solution weight 7 ‰ hydrolyzes 1h；It is heated to 100 DEG C after hydrolysis, keeps 20min enzyme deactivation；After enzyme deactivation rapidly
5 DEG C are cooled to, stichopus japonicus enzymolysis liquid is made；Wherein, compound protease is using the sale of Guangzhou Hua Qi Biotechnology Co., Ltd
Compound protease；The flavor protease that flavor protease uses the enzyme-linked biological study in Shanghai to be sold；
(2) preparation of Chinese medicine extract
Chinese medicine Schisandra chinensis, fructus lycii and wild chrysanthemum are chosen, by weight percentage are as follows: Schisandra chinensis 40%, fructus lycii 40% and open country
20% compatibility of chrysanthemum is at substrate raw material；Substrate raw material is changed water using hot water decocting method to extract three times, wherein substrate is added for the first time
The water that 8 times of raw material weight boils extraction 2 hours, is separated by solid-liquid separation, obtains first time Aqueous extracts；Second of addition substrate raw material weight 5
Water again boils extraction 1 hour, is separated by solid-liquid separation, obtains second of Aqueous extracts；3 times of substrate raw material weight of water is added in third time, boils
Boiling is extracted 1 hour, is separated by solid-liquid separation, and third time is obtained；It merges, must merge after first, second and third Aqueous extracts is filtered respectively
Liquid；After amalgamation liquid is concentrated at 80 DEG C using Rotary Evaporators, 50 DEG C are cooled to, being slowly added to concentration under stirring is
95% ethyl alcohol makes ethanol content in whole system reach 75%, and sealing is stood for 24 hours, and filtering takes supernatant using Rotary Evaporators
At 80 DEG C, ethyl alcohol is evaporated off in concentration, and adding pure water and adjusting solid content is 10%, and Chinese medicine extract is made；
(3) raw material mixing is prepared with finished product
Stichopus japonicus enzymolysis liquid made from weighing step (1), Chinese medicine extract made from step (2) mix in proportion, and stirring is equal
After even, be concentrated, centrifugation takes supernatant, add auxiliary material xylitol, after mixing evenly, successively through UHT sterilization, hot filling, secondary kill
After bacterium, protect liver stichopus japonicus traditional Chinese medicine oral liquid is made；Wherein, in the protect liver stichopus japonicus traditional Chinese medicine oral liquid, the weight percent of each component
It is respectively as follows: stichopus japonicus enzymolysis liquid 65%, Chinese medicine extract 25% and auxiliary material 10%.
Stichopus japonicus enzymolysis liquid and Chinese medicine extract collective effect in protect liver stichopus japonicus traditional Chinese medicine oral liquid manufactured in the present embodiment, shield
Nourishing liver effect is good, oral solution prescription science, sweet and sour taste, non-evident effect, can be used for preventing fatty liver, alcoholic liver,
The daily health carings such as drug induced hepatic injury.Preparation method rational technology, operation is feasible, is suitble to industrialized production.
Embodiment 3
A kind of preparation method of protect liver stichopus japonicus traditional Chinese medicine oral liquid, by following process steps:
(1) preparation of stichopus japonicus enzymolysis liquid
Choosing fresh stichopus japonicus is raw material, and stichopus japonicus is removed internal organs, and is cleaned, after taking the 85 DEG C of hot water blanchings of stichopus japonicus body wall,
Distilled water is added, grinds pulp using colloid mill, is deployed into the stichopus japonicus solution that protein concentration is 10%；Stichopus japonicus solution is heated
To 90 DEG C, 30min is kept, is subsequently cooled to 40 DEG C, the composite protease hydrolysis 1h of solution weight 7 ‰ is added, then adds
The flavor protease of solution weight 4 ‰ hydrolyzes 3h；It is heated to 90 DEG C after hydrolysis, keeps 30min enzyme deactivation；It is cold rapidly after enzyme deactivation
But to 3 DEG C, stichopus japonicus enzymolysis liquid is made；Wherein, compound protease believes compound protease using commercially available Novi；Flavor protease is adopted
Flavor protease is believed with commercially available Novi；
(2) preparation of Chinese medicine extract
Chinese medicine Schisandra chinensis, fructus lycii and wild chrysanthemum are chosen, by weight percentage are as follows: Schisandra chinensis 30%, fructus lycii 30% and open country
40% compatibility of chrysanthemum is at substrate raw material；Substrate raw material is changed water using hot water decocting method to extract three times, wherein substrate is added for the first time
The water that 10 times of raw material weight boils extraction 2 hours, is separated by solid-liquid separation, obtains first time Aqueous extracts；Second of addition substrate raw material weight
8 times of water boils extraction 1 hour, is separated by solid-liquid separation, obtains second of Aqueous extracts；6 times of substrate raw material weight of water is added in third time,
Extraction 1 hour is boiled, is separated by solid-liquid separation, obtains third time Aqueous extracts；It is merged after first, second and third Aqueous extracts is filtered respectively,
Obtain amalgamation liquid；After amalgamation liquid is concentrated at 80 DEG C using Rotary Evaporators, 30 DEG C are cooled to, is slowly added under stirring
Concentration is 95% ethyl alcohol, so that ethanol content in whole system is reached 70%, sealing stands 36h, and filtering takes supernatant using rotation
For evaporimeter at 80 DEG C, ethyl alcohol is evaporated off in concentration, and adding pure water and adjusting solid content is 8%, and Chinese medicine extract is made；
(3) raw material mixing is prepared with finished product
Stichopus japonicus enzymolysis liquid made from weighing step (1), Chinese medicine extract made from step (2) mix in proportion, and stirring is equal
It after even, is concentrated, centrifugation takes supernatant, auxiliary material sucrose is added, after mixing evenly, successively through UHT sterilization, hot filling, re-pasteurization
Afterwards, protect liver stichopus japonicus traditional Chinese medicine oral liquid is made；Wherein, in the protect liver stichopus japonicus traditional Chinese medicine oral liquid, the weight percent score of each component
Not are as follows: stichopus japonicus enzymolysis liquid 50%, Chinese medicine extract 45% and auxiliary material 5%.
Protect liver stichopus japonicus traditional Chinese medicine oral liquid manufactured in the present embodiment is by stichopus japonicus enzymolysis liquid and Schisandra chinensis, fructus lycii and wild chrysanthemum
The Chinese medicine extract compatibility produced, wherein stichopus japonicus enzymolysis liquid has reduction transaminase, improves the effect of immunity of organisms；The five tastes
Son plays the role of significant protecting liver, lowering enzymes, facilitates the synthesis of Hepatocyte matter；The substantially reduced large dosage of fructus lycii energy is drunk
With hepatic injury caused by carbon tetrachloride (CCl4)；The aqueous extract of wild chrysanthemum has clearing heat and detoxicating, dispelling wind calming the liver, improves immunity
The effect of.Each component compatibility plays synergistic effect, and protecting and nourishing liver effect is good, the oral solution prescription science, sweet and sour taste, without obvious
Side effect.Preparation method rational technology, operation is feasible, is suitble to industrialized production.
Animal experiment is carried out with stichopus japonicus traditional Chinese medicine oral liquid prepared by embodiment 1 below, it is of the invention further to prove
Stichopus japonicus traditional Chinese medicine oral liquid has effects that liver protecting.
Zoopery 1: pass through mouse CCl4Liver injury model observes stichopus japonicus extract oral by the way of oral administration
Liquid is to CCl4The serum alanine aminotransferase (ALT) of hepatic injury mouse, aspartate amino transferase (AST) index
Influence.
Kunming mice 40 are taken, experiment high dose group is randomly divided into, tests middle dose group, experiment low dose group and blank pair
According to group, totally 4 groups, every group 10.The high, medium and low dosage group of experimental group give respectively daily 0.025ml/g, 0.050ml/g,
The stichopus japonicus traditional Chinese medicine oral liquid stomach-filling of 0.075ml/g；Blank control group: the physiological saline stomach-filling of the 0.9% of same volume is given；It fills
Gastric cycle is surrounding.After surrounding, each experimental group group mouse presses 1h after former dosage stichopus japonicus traditional Chinese medicine oral liquid stomach-filling, abdominal cavity daily
Inject 0.1% CCl4Peanut oil solution, dosage are 10 ml/ kg；Equivalent peanut oil is injected intraperitoneally in control group.Continuous one week.It is real
The indices of each experimental group and control group mice are detected after testing respectively, comparing result obtains, high, medium and low dosage stichopus japonicus
Traditional Chinese medicine oral liquid can reduce in varying degrees serum alt, the activity of AST or content (P< 0.05).
Pharmacodynamic experiment shows the stichopus japonicus traditional Chinese medicine oral liquid to CCl4Hepatic injury mouse has significant protective effect.
Zoopery 2: it by alcohol-induced acute hepatic injury model, by the way of oral administration, observes in stichopus japonicus
Medicine oral solution is to the serum levels of triglyceride (TG) of acute alcohol-induced hepatic injury mouse, malonaldehyde (MDA) and glutathione (GSH)
The influence of content's index.
Kunming mice 40 are taken, experiment high dose group is randomly divided into, tests middle dose group, experiment low dose group and blank pair
According to group, totally 4 groups, every group 10.The high, medium and low dosage group of experimental group give respectively daily 0.025ml/g, 0.050ml/g,
The stichopus japonicus traditional Chinese medicine oral liquid stomach-filling of 0.075ml/g；Blank control group: the physiological saline stomach-filling of the 0.9% of same volume is given；It fills
Gastric cycle is surrounding.After surrounding, each experimental mice presses 1h after former dosage stichopus japonicus traditional Chinese medicine oral liquid stomach-filling, then stomach-filling daily
50 ° of white wine；Control group stomach-filling same amount of normal saline.Continuous one week.It is small with control group that each experimental group is detected after experiment respectively
The indices of mouse, comparing result obtain, and high, medium and low dosage stichopus japonicus traditional Chinese medicine oral liquid can reduce mouse blood in varying degrees
Clear MDA, TG activity or index (P< 0.05), improve liver GSH content (P <0.05)。
Pharmacodynamic experiment shows that the stichopus japonicus traditional Chinese medicine oral liquid there is apparent protection to make acute alcohol hepatic injury mouse
With.
Claims (6)
1. a kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid, it is characterised in that: it is made of stichopus japonicus enzymolysis liquid, Chinese medicine extract and auxiliary material,
Wherein, the weight percent of each component be respectively as follows: stichopus japonicus enzymolysis liquid 50~65%, Chinese medicine extract 25~45% and auxiliary material 5~
10%；The stichopus japonicus enzymolysis liquid is successively to use compound protease, flavor albumen by stichopus japonicus body wall after hot water blanching, homogenate
Enzyme carries out control enzyme hydrolysis, and enzyme deactivation is made after cooling；The Chinese medicine extract is to choose Chinese medicine Schisandra chinensis, fructus lycii, wild chrysanthemum
Compatibility extracts to use ethyl alcohol removal precipitating after obtaining Aqueous extracts using hot water at substrate raw material, after taking supernatant to remove ethyl alcohol, then
Pure water adjusting solid content is added to be made for 8~10%；The weight when Schisandra chinensis, fructus lycii, wild chrysanthemum compatibility
Percentage is respectively as follows: Schisandra chinensis 30~40%, fructus lycii 30~40% and wild chrysanthemum 20~40%.
2. a kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid according to claim 1, it is characterised in that: the protect liver stichopus japonicus Chinese medicine
The weight percentage of each component of oral solution is respectively as follows: stichopus japonicus enzymolysis liquid 60%, Chinese medicine extract 33% and auxiliary material 7%.
3. a kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid according to claim 1, it is characterised in that: the Schisandra chinensis, fructus lycii
Weight percent when son, wild chrysanthemum compatibility is respectively as follows: Schisandra chinensis 35%, fructus lycii 35% and wild chrysanthemum 30%.
4. a kind of protect liver stichopus japonicus traditional Chinese medicine oral liquid according to claim 1, it is characterised in that: the auxiliary material is xylose
Alcohol.
5. a kind of preparation method of protect liver stichopus japonicus traditional Chinese medicine oral liquid according to claim 1, it is characterised in that: by following
Processing step:
(1) preparation of stichopus japonicus enzymolysis liquid
Choosing fresh stichopus japonicus is raw material, and stichopus japonicus is removed internal organs, and is cleaned, and after taking stichopus japonicus body wall hot water blanching, distillation is added
Water grinds pulp using colloid mill, is deployed into the stichopus japonicus solution that protein concentration is 10~20%；Stichopus japonicus solution is heated to 90
~100 DEG C, 10~30min is kept, is subsequently cooled to 40~55 DEG C, the composite protease hydrolysis 1 of solution weight 4~7 ‰ is added
~3h, the flavor protease for then adding solution weight 4~7 ‰ hydrolyze 1~3h；90~100 DEG C are heated to after hydrolysis,
Keep 20~30min enzyme deactivation；It is rapidly cooled to 3~5 DEG C after enzyme deactivation, stichopus japonicus enzymolysis liquid is made；
(2) preparation of Chinese medicine extract
Chinese medicine Schisandra chinensis, fructus lycii and wild chrysanthemum are chosen, by weight percentage are as follows: Schisandra chinensis 30~40%, fructus lycii 30~40%
With 20~40% compatibility of wild chrysanthemum at substrate raw material；Substrate raw material is changed water using hot water decocting method to extract three times, water is made respectively
Extract；It Aqueous extracts will be merged after filtering respectively three times, and obtain amalgamation liquid；Amalgamation liquid is dense at 80 DEG C using Rotary Evaporators
After contracting, 30~50 DEG C are cooled to, it is 95% ethyl alcohol that concentration is slowly added under stirring, reaches ethanol content in whole system
To 70~75%, sealing stands 24~36h, and filtering takes supernatant using Rotary Evaporators at 80 DEG C, and ethyl alcohol is evaporated off in concentration,
Adding pure water and adjusting solid content is 8~10%, and Chinese medicine extract is made；
(3) raw material mixing is prepared with finished product
Stichopus japonicus enzymolysis liquid made from weighing step (1), Chinese medicine extract made from step (2) mix in proportion, after mixing evenly,
Concentration, centrifugation take supernatant, add auxiliary material, after mixing evenly, successively after UHT sterilization, hot filling, re-pasteurization, shield are made
Liver stichopus japonicus traditional Chinese medicine oral liquid；Wherein, in the protect liver stichopus japonicus traditional Chinese medicine oral liquid, the weight percent of each component is respectively as follows: stichopus japonicus
Enzymolysis liquid 50~65%, Chinese medicine extract 25~45% and auxiliary material 5~10%.
6. a kind of preparation method of protect liver stichopus japonicus traditional Chinese medicine oral liquid according to claim 5, it is characterised in that: the general
It is to decoct substrate raw material boiling water three times three times that substrate raw material, which changes water extraction using hot water decocting method, wherein bottom is added for the first time
The water that 8~10 times of raw material weight boils extraction 2 hours, is separated by solid-liquid separation；Second of 5~8 times of substrate raw material weight of addition
Water boils extraction 1 hour, is separated by solid-liquid separation；3~6 times of substrate raw material weight of water is added in third time, boils extraction 1 hour, solid-liquid
Separation.
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| "Three cerebrosides from the sea cucumber Cucumaria frondosa";Ming-PingLA，等;《Chinese Journal of Natural Medicines》;20120331;第10卷(第2期);Pages 105-109 |
| "刺参糖胺聚糖对HBV转基因小鼠肝组织HBV相关抗原表达的影响";周丽，等;《世界华人消化杂志 》;20101231(第12期) |
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