CN104435359A - Liver-protecting stichopus japonicus traditional Chinese medicine oral solution and preparation method thereof - Google Patents
Liver-protecting stichopus japonicus traditional Chinese medicine oral solution and preparation method thereof Download PDFInfo
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- CN104435359A CN104435359A CN201410744941.6A CN201410744941A CN104435359A CN 104435359 A CN104435359 A CN 104435359A CN 201410744941 A CN201410744941 A CN 201410744941A CN 104435359 A CN104435359 A CN 104435359A
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- morinae bulleyanae
- radix morinae
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Classifications
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/616—Echinodermata, e.g. starfish, sea cucumbers or sea urchins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/287—Chrysanthemum, e.g. daisy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
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- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
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- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
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- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a liver-protecting stichopus japonicus traditional Chinese medicine oral solution and a preparation method thereof. The method comprises the following steps: blanching and homogenating the wall of a stichopus japonicus body, carrying out controlled enzyme hydrolysis sequentially by compound protease and flavourzyme, deactivating enzyme, and cooling to prepare a stichopus japonicus enzymatic hydrolysate; preparing a substrate raw material from schisandra chinensis, fructus lycii and chrysanthemum indicum, obtaining a water extract by using a hot-water decoction method, removing precipitate with ethanol, distilling supernate to remove ethanol, and adding pure water to prepare a traditional Chinese medicine extract; mixing the stichopus japonicus enzymatic hydrolysate and the traditional Chinese medicine extract, preparing, concentrating, centrifuging to obtain supernate, adding auxiliaries, uniformly stirring, and sequentially carrying out UHT sterilization, hot filling and secondary sterilization to prepare the liver-protecting stichopus japonicus traditional Chinese medicine oral solution. The liver-protecting stichopus japonicus traditional Chinese medicine oral solution is scientific in formula, sour and sweet, tastes delicious, has the effects of soothing-liver, regulating qi, clearing heat, removing toxicity, calming the liver and tranquilizing, and has double functions of health protection and nourishing. The preparation method is reasonable in process and feasible in operation, and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of pharmaceutical preparation not determining structure of traditional herbal medicines, especially a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid and preparation method thereof.
Background technology
Along with the development of society, growth in the living standard, the crowd facing hepar damnification is more and more huger.Excessive consumption of alcohol, Drug abuse, diet is greasy, is all the inducement causing hepar damnification.How hepatoprotective becomes problem demanding prompt solution at present.
Stichopus japonicus has another name called Radix Morinae Bulleyanae, extra large Mus, sea gherkin etc., is a kind of famous and precious marine animal, because of its benefiting action, is similar to Radix Ginseng and gains the name.Stichopus japonicus is not only precious food, is also famous and precious medical material.Record according in supplementary Amplifications of the Compendium of Materia Medica: Stichopus japonicus, sweet-salty, the kidney invigorating, beneficial marrow, takes the photograph urine, and flaccidity is treated in tonifying YANG, its benefit warm in nature, sufficient enemy's ginseng, therefore named Stichopus japonicus.Here the Stichopus japonicus said just refers to Radix Morinae Bulleyanae.Verify after deliberation: be rich in the various active materials such as saponin, mucopolysaccharide, ganglioside, sterol, collagen protein in Radix Morinae Bulleyanae body, make it have many-sided health-care effect, comprise hepatoprotective aspect.Large quantity research shows, the mouse liver function damage effect of being significantly improved that the fucoidan (SC-FUC) in Radix Morinae Bulleyanae causes long-term alcohol; Cerebroside (SCC) is to carbon tetrachloride (CCl
4) cause the acute liver injury of rats effect of being significantly improved; Acidic mucopolysaccharide (SJAMP) has suppression to do to diethylnitrosamine (DEN) induced rat genesis of HCC.In addition, Radix Morinae Bulleyanae significantly can also regulate the blood lipid level of body, effectively regulates hypercholesterolemicrats rats lipid metabolism.Visible, the mystery effect of Radix Morinae Bulleyanae in the liver protecting, is constantly found.And Radix Morinae Bulleyanae also has good trophism, it is rich in 18 seed amino acids, the macroelement of multiple needed by human and trace element, and multivitamin.
And utilize Radix Morinae Bulleyanae to report to some extent for raw material prepares medicine in open file, such as, disclosed number of patent application is: the Chinese invention patent application of 200810238717.4, disclose " a kind of Apostichopus japonicus nourishing granules, the processing method of capsule and Apostichopus japonicus nourishing granules ", it utilizes fresh Radix Morinae Bulleyanae for primary raw material, rich in proteins is obtained by enzymolysis, peptide class, the enzymolysis solution of aminoacid and Saponin and Radix Morinae Bulleyanae mucopolysaccharide isoreactivity composition, dry after the technical finesse of cyclodextrin bioencapsulation, again with Radix Et Caulis Acanthopanacis Senticosi, the Radix Astragali, the Chinese medicine extract mixing such as Radix Glycyrrhizae, through granulating, obtained after the processed such as drying, there is nutritious tonifying, anti-ageing liter is white, improve health care and the therapeutical effect such as immunity.
At present, also do not find that utilizing Radix Morinae Bulleyanae to arrange in pairs or groups for raw material and other Chinese medicine prepares the report of liver-care preparations.
Summary of the invention
The object of this invention is to provide a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid and preparation method thereof, this hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid scientific formula, protecting and nourishing liver is effective, non-evident effect; Its preparation method rational technology, operation feasible, applicable suitability for industrialized production.
The technical solution adopted for the present invention to solve the technical problems is: a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, it is characterized in that: it comprises Radix Morinae Bulleyanae enzymolysis solution, Chinese medicine extraction liquid and adjuvant, wherein, the percentage by weight of each component is respectively: Radix Morinae Bulleyanae enzymolysis solution 50 ~ 65%, Chinese medicine extraction liquid 25 ~ 45% and adjuvant 5 ~ 10%; Described Radix Morinae Bulleyanae enzymolysis solution is by Radix Morinae Bulleyanae body wall after hot water blanching, homogenate, adopts compound protease, flavor protease to carry out control enzyme hydrolysis, enzyme denaturing successively, obtained after cooling; Described Chinese medicine extraction liquid be choose Chinese medicine Schisandra chinensis, Fructus Lycii, Flos Chrysanthemi Indici compatibility become substrate raw material, ethanol is adopted to remove precipitation after adopting hot water extraction to obtain Aqueous extracts, get supernatant to remove after ethanol, then add pure water and regulate solid content to be 8 ~ 10% to obtain; Percentage by weight when described Fructus Schisandrae Chinensis, Fructus Lycii, Flos Chrysanthemi Indici compatibility is respectively: Fructus Schisandrae Chinensis 30 ~ 40%, Fructus Lycii 30 ~ 40% and Flos Chrysanthemi Indici 20 ~ 40%.
Priority scheme of the present invention: each weight percentages of components of described hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid is respectively: Radix Morinae Bulleyanae enzymolysis solution 60%, Chinese medicine extraction liquid 33% and adjuvant 7%.
Percentage by weight when described Fructus Schisandrae Chinensis, Fructus Lycii, Flos Chrysanthemi Indici compatibility is respectively: Fructus Schisandrae Chinensis 35%, Fructus Lycii 35% and Flos Chrysanthemi Indici 30%.
Described adjuvant is xylitol.
A preparation method for hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, is characterized in that: through following process steps:
(1) preparation of Radix Morinae Bulleyanae enzymolysis solution
Choosing fresh Radix Morinae Bulleyanae is raw material, is gilled by Radix Morinae Bulleyanae, and cleaning, after getting the blanching of Radix Morinae Bulleyanae body wall hot water, adds distilled water, utilize colloid mill to grind to form pulpous state, be deployed into the Radix Morinae Bulleyanae solution that protein concentration is 10 ~ 20%; Radix Morinae Bulleyanae solution is heated to 90 ~ 100 DEG C, keeps 10 ~ 30min, be then cooled to 40 ~ 55 DEG C, add the composite protease hydrolysis 1 ~ 3h of solution weight 4 ~ 7 ‰, and then add the flavor protease hydrolysis 1 ~ 3h of solution weight 4 ~ 7 ‰; Be heated to 90 ~ 100 DEG C after hydrolysis, keep 20 ~ 30min enzyme denaturing; 3 ~ 5 DEG C are cooled to rapidly, obtained Radix Morinae Bulleyanae enzymolysis solution after enzyme denaturing;
(2) preparation of Chinese medicine extraction liquid
Choose Chinese medicine Schisandra chinensis, Fructus Lycii and Flos Chrysanthemi Indici, be by weight percentage: Fructus Schisandrae Chinensis 30 ~ 40%, Fructus Lycii 30 ~ 40% become substrate raw material with Flos Chrysanthemi Indici 20 ~ 40% compatibility; Hot water decocting method is adopted to change water extraction three times substrate raw material, respectively obtained Aqueous extracts; Merge after three Aqueous extracts are filtered respectively, obtain amalgamation liquid; After being adopted by amalgamation liquid Rotary Evaporators concentrated at 80 DEG C, be cooled to 30 ~ 50 DEG C, under stirring, slowly add concentration is 95% ethanol, makes ethanol content in whole system reach 70 ~ 75%, sealing, leave standstill 24 ~ 36h, filter, get supernatant and adopt Rotary Evaporators at 80 DEG C, concentrated steaming removes ethanol, adding pure water again regulates solid content to be 8 ~ 10%, obtained Chinese medicine extraction liquid;
(3) raw material mixing is prepared with finished product
The Chinese medicine extraction liquid that the Radix Morinae Bulleyanae enzymolysis solution that weighing step (1) obtains, step (2) obtain is mixed in proportion, after stirring, concentrated, centrifugal, get supernatant, add adjuvant, after stirring, successively after UHT sterilization, hot filling, re-pasteurization, obtained hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid; Wherein, in this hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, the percentage by weight of each component is respectively: Radix Morinae Bulleyanae enzymolysis solution 50 ~ 65%, Chinese medicine extraction liquid 25 ~ 45% and adjuvant 5 ~ 10%.
Described to adopt hot water decocting method to change water extraction substrate raw material be for three times that substrate raw material boiling water is decocted three times, and wherein first time adds the water of substrate raw material weight 8 ~ 10 times, boils extraction 2 hours, solid-liquid separation; Second time adds the water of substrate raw material weight 5 ~ 8 times, boils extraction 1 hour, solid-liquid separation; The water that third time adds substrate raw material weight 3 ~ 6 times, boils extraction 1 hour, solid-liquid separation.
Hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid of the present invention comprises the Chinese medicine extraction liquid and adjuvant that Radix Morinae Bulleyanae enzymolysis solution, Fructus Schisandrae Chinensis, Fructus Lycii and Flos Chrysanthemi Indici produce, wherein, Radix Morinae Bulleyanae enzymolysis solution is aminoacid, polypeptide, the polyoses extract produced after double enzymolysis by Radix Morinae Bulleyanae body wall, be conducive to the absorption of human body, there is reduction transaminase, improve the effect of immunity of organisms.Chinese medicine Schisandra chinensis and the Flos Chrysanthemi Indici of producing Chinese medicine extraction liquid are the medicines that Ministry of Public Health ratifies can be used for health food, and Fructus Lycii is then the food of medicine-food two-purpose.Wherein, the main component in Fructus Schisandrae Chinensis is lignanoids material, has the effect of significant the liver protecting and ALT lowering, contributes to the synthesis of Hepatocyte matter, obviously can reduce serum transaminase, effectively repair damaged liver; Fructus Lycii obviously can alleviate heavy dose and drink and the hepatic injury caused by carbon tetrachloride (CCl4); The aqueous extract of Flos Chrysanthemi Indici has heat-clearing and toxic substances removing, dispelling wind suppressing the hyperactive liver, improves effect of immunity.The common compatibility of Chinese medicine extraction liquid that Radix Morinae Bulleyanae enzymolysis and three taste Chinese medicines are produced, produces synergism, has depressed liver-energy dispersing and QI regulating, effect that heat-clearing and toxic substances removing, suppressing the hyperactive liver are calmed the nerves.Show through zoopery, its liver protection function is given prominence to.This hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid scientific formula, sweet and sour taste, non-evident effect, can be used for preventing the daily health caring of fatty liver, alcoholic liver, drug induced hepatic injury etc. and separating greasy after the meal, especially suitable long-term excessive consumption of alcohol, Drug abuse, crowd that diet is greasy.Preparation method rational technology of the present invention, operation feasible, applicable suitability for industrialized production.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
embodiment 1
A preparation method for hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, through following process steps:
(1) preparation of Radix Morinae Bulleyanae enzymolysis solution
Choosing fresh Radix Morinae Bulleyanae is raw material, is gilled by Radix Morinae Bulleyanae, and cleaning, gets Radix Morinae Bulleyanae body wall with after 80 DEG C of hot water blanchings, add distilled water, utilize colloid mill to grind to form pulpous state, be deployed into the Radix Morinae Bulleyanae solution that protein concentration is 15%; Radix Morinae Bulleyanae solution is heated to 95 DEG C, keeps 20min, be then cooled to 50 DEG C, add the composite protease hydrolysis 2h of solution weight 5 ‰, and then add the flavor protease hydrolysis 2h of solution weight 6 ‰; Be heated to 95 DEG C after hydrolysis, keep 25min enzyme denaturing; 4 DEG C are cooled to rapidly, obtained Radix Morinae Bulleyanae enzymolysis solution after enzyme denaturing; Wherein, compound protease adopts commercially available Novi's letter compound protease; Flavor protease adopts commercially available Novi's letter flavor protease;
(2) preparation of Chinese medicine extraction liquid
Choose Chinese medicine Schisandra chinensis, Fructus Lycii and Flos Chrysanthemi Indici, be by weight percentage: Fructus Schisandrae Chinensis 35%, Fructus Lycii 35% become substrate raw material with Flos Chrysanthemi Indici 30% compatibility; Adopt hot water decocting method to change water extraction three times substrate raw material, wherein, the water that first time adds substrate raw material weight 9 times, boils extraction 2 hours, solid-liquid separation, obtains first time Aqueous extracts; Second time adds the water of substrate raw material weight 6 times, boils extraction 1 hour, solid-liquid separation, obtains second time Aqueous extracts; The water that third time adds substrate raw material weight 4 times, boils extraction 1 hour, solid-liquid separation, obtains third time Aqueous extracts; Merge after first, second and third Aqueous extracts is filtered respectively, obtain amalgamation liquid; After being adopted by amalgamation liquid Rotary Evaporators concentrated at 80 DEG C, be cooled to 40 DEG C, under stirring, slowly add concentration is 95% ethanol, makes ethanol content in whole system reach 72%, sealing, leave standstill 30h, filter, get supernatant and adopt Rotary Evaporators at 80 DEG C, concentrated steaming removes ethanol, adding pure water again regulates solid content to be 9%, obtained Chinese medicine extraction liquid;
(3) raw material mixing is prepared with finished product
The Chinese medicine extraction liquid that the Radix Morinae Bulleyanae enzymolysis solution that weighing step (1) obtains, step (2) obtain is mixed in proportion, after stirring, concentrated, centrifugal, get supernatant, add adjuvant xylitol, after stirring, successively after UHT sterilization, hot filling, re-pasteurization, obtained hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid; Wherein, in this hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, the percentage by weight of each component is respectively: Radix Morinae Bulleyanae enzymolysis solution 60%, Chinese medicine extraction liquid 33% and adjuvant 7%.
In hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid prepared by the present embodiment, Radix Morinae Bulleyanae enzymolysis solution with there is the common compatibility of Chinese medicine extraction liquid that synergistic Fructus Schisandrae Chinensis, Fructus Lycii and Flos Chrysanthemi Indici Chinese medicine produces, there is depressed liver-energy dispersing and QI regulating, effect that heat-clearing and toxic substances removing, suppressing the hyperactive liver are calmed the nerves, possess the dual function of health care and nutrition.This hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid scientific formula, sweet and sour taste, protecting and nourishing liver is effective, has no side effect, and can be used for separating the daily health carings such as greasy, prevention fatty liver, alcoholic liver, drug induced hepatic injury after the meal.Its preparation method rational technology, operation feasible, applicable suitability for industrialized production.
embodiment 2
A preparation method for hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, through following process steps:
(1) preparation of Radix Morinae Bulleyanae enzymolysis solution
Choosing fresh Radix Morinae Bulleyanae is raw material, is gilled by Radix Morinae Bulleyanae, and cleaning, gets Radix Morinae Bulleyanae body wall with after 90 DEG C of hot water blanchings, add distilled water, utilize colloid mill to grind to form pulpous state, be deployed into the Radix Morinae Bulleyanae solution that protein concentration is 20%; Radix Morinae Bulleyanae solution is heated to 100 DEG C, keeps 10min, be then cooled to 55 DEG C, add the composite protease hydrolysis 3h of solution weight 4 ‰, and then add the flavor protease hydrolysis 1h of solution weight 7 ‰; Be heated to 100 DEG C after hydrolysis, keep 20min enzyme denaturing; 5 DEG C are cooled to rapidly, obtained Radix Morinae Bulleyanae enzymolysis solution after enzyme denaturing; Wherein, compound protease adopts the compound protease that Hua Qi bio tech ltd, Guangzhou sells; The flavor protease that flavor protease adopts Shanghai enzyme connection biological study to sell;
(2) preparation of Chinese medicine extraction liquid
Choose Chinese medicine Schisandra chinensis, Fructus Lycii and Flos Chrysanthemi Indici, be by weight percentage: Fructus Schisandrae Chinensis 40%, Fructus Lycii 40% become substrate raw material with Flos Chrysanthemi Indici 20% compatibility; Adopt hot water decocting method to change water extraction three times substrate raw material, wherein first time adds the water of substrate raw material weight 8 times, boils extraction 2 hours, solid-liquid separation, obtains first time Aqueous extracts; Second time adds the water of substrate raw material weight 5 times, boils extraction 1 hour, solid-liquid separation, obtains second time Aqueous extracts; The water that third time adds substrate raw material weight 3 times, boils extraction 1 hour, solid-liquid separation, obtains third time; Merge after first, second and third Aqueous extracts is filtered respectively, obtain amalgamation liquid; After being adopted by amalgamation liquid Rotary Evaporators concentrated at 80 DEG C, be cooled to 50 DEG C, under stirring, slowly add concentration is 95% ethanol, makes ethanol content in whole system reach 75%, sealing, leave standstill 24h, filter, get supernatant and adopt Rotary Evaporators at 80 DEG C, concentrated steaming removes ethanol, adding pure water again regulates solid content to be 10%, obtained Chinese medicine extraction liquid;
(3) raw material mixing is prepared with finished product
The Chinese medicine extraction liquid that the Radix Morinae Bulleyanae enzymolysis solution that weighing step (1) obtains, step (2) obtain is mixed in proportion, after stirring, concentrated, centrifugal, get supernatant, add adjuvant xylitol, after stirring, successively after UHT sterilization, hot filling, re-pasteurization, obtained hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid; Wherein, in this hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, the percentage by weight of each component is respectively: Radix Morinae Bulleyanae enzymolysis solution 65%, Chinese medicine extraction liquid 25% and adjuvant 10%.
Radix Morinae Bulleyanae enzymolysis solution and Chinese medicine extraction liquid combined effect in hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid prepared by the present embodiment, its protecting and nourishing liver is effective, this oral liquid scientific formula, sweet and sour taste, non-evident effect, can be used for the daily health carings such as prevention fatty liver, alcoholic liver, drug induced hepatic injury.Its preparation method rational technology, operation feasible, applicable suitability for industrialized production.
embodiment 3
A preparation method for hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, through following process steps:
(1) preparation of Radix Morinae Bulleyanae enzymolysis solution
Choosing fresh Radix Morinae Bulleyanae is raw material, is gilled by Radix Morinae Bulleyanae, and cleaning, gets Radix Morinae Bulleyanae body wall with after 85 DEG C of hot water blanchings, add distilled water, utilize colloid mill to grind to form pulpous state, be deployed into the Radix Morinae Bulleyanae solution that protein concentration is 10%; Radix Morinae Bulleyanae solution is heated to 90 DEG C, keeps 30min, be then cooled to 40 DEG C, add the composite protease hydrolysis 1h of solution weight 7 ‰, and then add the flavor protease hydrolysis 3h of solution weight 4 ‰; Be heated to 90 DEG C after hydrolysis, keep 30min enzyme denaturing; 3 DEG C are cooled to rapidly, obtained Radix Morinae Bulleyanae enzymolysis solution after enzyme denaturing; Wherein, compound protease adopts commercially available Novi's letter compound protease; Flavor protease adopts commercially available Novi's letter flavor protease;
(2) preparation of Chinese medicine extraction liquid
Choose Chinese medicine Schisandra chinensis, Fructus Lycii and Flos Chrysanthemi Indici, be by weight percentage: Fructus Schisandrae Chinensis 30%, Fructus Lycii 30% become substrate raw material with Flos Chrysanthemi Indici 40% compatibility; Adopt hot water decocting method to change water extraction three times substrate raw material, wherein first time adds the water of substrate raw material weight 10 times, boils extraction 2 hours, solid-liquid separation, obtains first time Aqueous extracts; Second time adds the water of substrate raw material weight 8 times, boils extraction 1 hour, solid-liquid separation, obtains second time Aqueous extracts; The water that third time adds substrate raw material weight 6 times, boils extraction 1 hour, solid-liquid separation, obtains third time Aqueous extracts; Merge after first, second and third Aqueous extracts is filtered respectively, obtain amalgamation liquid; After being adopted by amalgamation liquid Rotary Evaporators concentrated at 80 DEG C, be cooled to 30 DEG C, under stirring, slowly add concentration is 95% ethanol, makes ethanol content in whole system reach 70%, sealing, leave standstill 36h, filter, get supernatant and adopt Rotary Evaporators at 80 DEG C, concentrated steaming removes ethanol, adding pure water again regulates solid content to be 8%, obtained Chinese medicine extraction liquid;
(3) raw material mixing is prepared with finished product
The Chinese medicine extraction liquid that the Radix Morinae Bulleyanae enzymolysis solution that weighing step (1) obtains, step (2) obtain is mixed in proportion, after stirring, concentrated, centrifugal, get supernatant, add adjuvant sucrose, after stirring, successively after UHT sterilization, hot filling, re-pasteurization, obtained hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid; Wherein, in this hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, the percentage by weight of each component is respectively: Radix Morinae Bulleyanae enzymolysis solution 50%, Chinese medicine extraction liquid 45% and adjuvant 5%.
Hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid prepared by the present embodiment is Chinese medicine extraction liquid compatibility Radix Morinae Bulleyanae enzymolysis solution and Fructus Schisandrae Chinensis, Fructus Lycii and Flos Chrysanthemi Indici produced, and wherein, Radix Morinae Bulleyanae enzymolysis solution has reduction transaminase, improves the effect of immunity of organisms; Fructus Schisandrae Chinensis has the effect of significant the liver protecting and ALT lowering, contributes to the synthesis of Hepatocyte matter; Fructus Lycii obviously can alleviate heavy dose and drink and the hepatic injury caused by carbon tetrachloride (CCl4); The aqueous extract of Flos Chrysanthemi Indici has heat-clearing and toxic substances removing, dispelling wind suppressing the hyperactive liver, improves effect of immunity.Each component compatibility plays synergism, and protecting and nourishing liver is effective, this oral liquid scientific formula, sweet and sour taste, non-evident effect.Its preparation method rational technology, operation feasible, applicable suitability for industrialized production.
Animal experiment is carried out below, to prove that Radix Morinae Bulleyanae Chinese medicine oral liquid of the present invention has effect of hepatoprotective further with the Radix Morinae Bulleyanae Chinese medicine oral liquid prepared by embodiment 1.
Zoopery 1: by mice CCl
4liver injury model, adopts the mode of oral administration, observes Radix Morinae Bulleyanae Chinese medicine oral liquid to CCl
4the serum alanine aminotransferase (ALT) of hepatic injury mice, the impact of aspartate amino transferase (AST) index.
Get kunming mice 40, be divided into experiment high dose group at random, dosage group in experiment, experiment low dose group and blank group, totally 4 groups, often organize 10.The high, medium and low dosage component of experimental group does not give the Radix Morinae Bulleyanae Chinese medicine oral liquid gavage of every day 0.025ml/g, 0.050ml/g, 0.075ml/g; Blank group: the normal saline gavage giving 0.9% of same volume; The gavage cycle is surrounding.After surrounding, each experimental group group mice every day is by 1h, lumbar injection 0.1% CCl after former dosage Radix Morinae Bulleyanae Chinese medicine oral liquid gavage
4peanut oil solution, dosage is 10 ml/ kg; Matched group lumbar injection equivalent Oleum Arachidis hypogaeae semen.Continuous one week.Experiment terminates the rear indices detecting each experimental group and control group mice respectively, and comparing result obtains, high, medium and low dosage Radix Morinae Bulleyanae Chinese medicine oral liquid all can reduce in varying degrees serum alt, the activity of AST or content (
p< 0.05).
Pharmacodynamic experiment shows, this Radix Morinae Bulleyanae Chinese medicine oral liquid is to CCl
4hepatic injury mice has significant protective effect.
Zoopery 2: by alcohol-induced acute hepatic injury model, adopt the mode of oral administration, observe the impact of Radix Morinae Bulleyanae Chinese medicine oral liquid on the serum levels of triglyceride (TG) of acute alcohol-induced hepatic injury mice, malonaldehyde (MDA) and glutathion (GSH) content's index.
Get kunming mice 40, be divided into experiment high dose group at random, dosage group in experiment, experiment low dose group and blank group, totally 4 groups, often organize 10.The high, medium and low dosage component of experimental group does not give the Radix Morinae Bulleyanae Chinese medicine oral liquid gavage of every day 0.025ml/g, 0.050ml/g, 0.075ml/g; Blank group: the normal saline gavage giving 0.9% of same volume; The gavage cycle is surrounding.After surrounding, each experimental mice every day is by 1h after former dosage Radix Morinae Bulleyanae Chinese medicine oral liquid gavage, then gavage 50 ° of Chinese liquor; Matched group gavage normal saline.Continuous one week.Experiment terminate the rear indices detecting each experimental group and control group mice respectively, comparing result obtains, high, medium and low dosage Radix Morinae Bulleyanae Chinese medicine oral liquid all can reduce in varying degrees mice serum MDA, TG activity or index (
p<0.05), improve liver GSH content (
p<0.05).
Pharmacodynamic experiment shows, this Radix Morinae Bulleyanae Chinese medicine oral liquid has significant protective effect to acute alcohol hepatic injury mice.
Claims (6)
1. a hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, is characterized in that: it comprises Radix Morinae Bulleyanae enzymolysis solution, Chinese medicine extraction liquid and adjuvant, and wherein, the percentage by weight of each component is respectively: Radix Morinae Bulleyanae enzymolysis solution 50 ~ 65%, Chinese medicine extraction liquid 25 ~ 45% and adjuvant 5 ~ 10%; Described Radix Morinae Bulleyanae enzymolysis solution is by Radix Morinae Bulleyanae body wall after hot water blanching, homogenate, adopts compound protease, flavor protease to carry out control enzyme hydrolysis, enzyme denaturing successively, obtained after cooling; Described Chinese medicine extraction liquid be choose Chinese medicine Schisandra chinensis, Fructus Lycii, Flos Chrysanthemi Indici compatibility become substrate raw material, ethanol is adopted to remove precipitation after adopting hot water extraction to obtain Aqueous extracts, get supernatant to remove after ethanol, then add pure water and regulate solid content to be 8 ~ 10% to obtain; Percentage by weight when described Fructus Schisandrae Chinensis, Fructus Lycii, Flos Chrysanthemi Indici compatibility is respectively: Fructus Schisandrae Chinensis 30 ~ 40%, Fructus Lycii 30 ~ 40% and Flos Chrysanthemi Indici 20 ~ 40%.
2. a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid according to claim 1, is characterized in that: each weight percentages of components of described hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid is respectively: Radix Morinae Bulleyanae enzymolysis solution 60%, Chinese medicine extraction liquid 33% and adjuvant 7%.
3. a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid according to claim 1, is characterized in that: percentage by weight when described Fructus Schisandrae Chinensis, Fructus Lycii, Flos Chrysanthemi Indici compatibility is respectively: Fructus Schisandrae Chinensis 35%, Fructus Lycii 35% and Flos Chrysanthemi Indici 30%.
4. a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid according to claim 1, is characterized in that: described adjuvant is xylitol.
5. the preparation method of a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid according to claim 1, is characterized in that: through following process steps:
(1) preparation of Radix Morinae Bulleyanae enzymolysis solution
Choosing fresh Radix Morinae Bulleyanae is raw material, is gilled by Radix Morinae Bulleyanae, and cleaning, after getting the blanching of Radix Morinae Bulleyanae body wall hot water, adds distilled water, utilize colloid mill to grind to form pulpous state, be deployed into the Radix Morinae Bulleyanae solution that protein concentration is 10 ~ 20%; Radix Morinae Bulleyanae solution is heated to 90 ~ 100 DEG C, keeps 10 ~ 30min, be then cooled to 40 ~ 55 DEG C, add the composite protease hydrolysis 1 ~ 3h of solution weight 4 ~ 7 ‰, and then add the flavor protease hydrolysis 1 ~ 3h of solution weight 4 ~ 7 ‰; Be heated to 90 ~ 100 DEG C after hydrolysis, keep 20 ~ 30min enzyme denaturing; 3 ~ 5 DEG C are cooled to rapidly, obtained Radix Morinae Bulleyanae enzymolysis solution after enzyme denaturing;
(2) preparation of Chinese medicine extraction liquid
Choose Chinese medicine Schisandra chinensis, Fructus Lycii and Flos Chrysanthemi Indici, be by weight percentage: Fructus Schisandrae Chinensis 30 ~ 40%, Fructus Lycii 30 ~ 40% become substrate raw material with Flos Chrysanthemi Indici 20 ~ 40% compatibility; Hot water decocting method is adopted to change water extraction three times substrate raw material, respectively obtained Aqueous extracts; Merge after three Aqueous extracts are filtered respectively, obtain amalgamation liquid; After being adopted by amalgamation liquid Rotary Evaporators concentrated at 80 DEG C, be cooled to 30 ~ 50 DEG C, under stirring, slowly add concentration is 95% ethanol, makes ethanol content in whole system reach 70 ~ 75%, sealing, leave standstill 24 ~ 36h, filter, get supernatant and adopt Rotary Evaporators at 80 DEG C, concentrated steaming removes ethanol, adding pure water again regulates solid content to be 8 ~ 10%, obtained Chinese medicine extraction liquid;
(3) raw material mixing is prepared with finished product
The Chinese medicine extraction liquid that the Radix Morinae Bulleyanae enzymolysis solution that weighing step (1) obtains, step (2) obtain is mixed in proportion, after stirring, concentrated, centrifugal, get supernatant, add adjuvant, after stirring, successively after UHT sterilization, hot filling, re-pasteurization, obtained hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid; Wherein, in this hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid, the percentage by weight of each component is respectively: Radix Morinae Bulleyanae enzymolysis solution 50 ~ 65%, Chinese medicine extraction liquid 25 ~ 45% and adjuvant 5 ~ 10%.
6. the preparation method of a kind of hepatoprotective Radix Morinae Bulleyanae Chinese medicine oral liquid according to claim 5, it is characterized in that: described to adopt hot water decocting method to change water extraction substrate raw material be for three times that substrate raw material boiling water is decocted three times, wherein first time adds the water of substrate raw material weight 8 ~ 10 times, boil extraction 2 hours, solid-liquid separation; Second time adds the water of substrate raw material weight 5 ~ 8 times, boils extraction 1 hour, solid-liquid separation; The water that third time adds substrate raw material weight 3 ~ 6 times, boils extraction 1 hour, solid-liquid separation.
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