CN102302427B - Production and extraction method of nostoc nourishing and moisturizing material - Google Patents
Production and extraction method of nostoc nourishing and moisturizing material Download PDFInfo
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- CN102302427B CN102302427B CN 201110284380 CN201110284380A CN102302427B CN 102302427 B CN102302427 B CN 102302427B CN 201110284380 CN201110284380 CN 201110284380 CN 201110284380 A CN201110284380 A CN 201110284380A CN 102302427 B CN102302427 B CN 102302427B
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- nostoc
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- 241000192656 Nostoc Species 0.000 title claims abstract description 94
- 230000003020 moisturizing effect Effects 0.000 title claims abstract description 28
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 8
- 239000000463 material Substances 0.000 title abstract description 4
- 238000000605 extraction Methods 0.000 title abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000000284 extract Substances 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 23
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 6
- 230000008030 elimination Effects 0.000 claims description 4
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- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 2
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- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
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- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a production and extraction method of a nostoc nourishing and moisturizing material. In the method, red light LED (light emitting diode) irradiation is added based on natural light or fluorescent lamp irradiation in the process of culturing nostoc to change nostoc polysaccharide molecules, so that hardness of the sphaeroid colony of fresh nostoc is increased, water content is reduced to 96 percent, and the biomass dry weight of the fresh product is increased to 4 percent; after being inactivated at a high temperature, the harvested nostoc colony is irradiated for 48 hours with strong blue light to fade pigments, and the nostoc is turned into a colorless sphere; the nostoc is homogenized and filtered, and then boiled with water and extracted for three times; and trichloroacetic acid accounting for 5 percent of the weight of the nostoc is added, and the mixture is stirred and filtered to remove protein. The method has the advantages that: the dry matters of the nostoc are increased by using the method, so that the production cost is greatly reduced, and the economical benefit is improved; and the nostoc nourishing and moisturizing extract has the beautifying functions of nourishing and moisturizing, and can be used as a raw material of cosmetics and skin-care products to play effects of maintaining skin moisture, nourishing skin to accelerate repairing.
Description
Technical field
The present invention relates to a kind of production and extracting method of Nostoc nutrient substance, belong to biological technical field.
Background technology
Nostoc (Nostoc sphaeroids Kutz), it is a kind of fresh water algae plant, it is a kind of many cells filamentous cyanobacteria, belong to Cyanophyta (Cyanophyta), Cyanophyceae (Cyanophyceae) together with Nostoc commune (Nostoc commune), deliver vegetables (Nostoc flagelliforme), section is grown Cutleriales (Hormogonales), nostocaceae (Nostocaceae) plant, formal name used at school is for intending ball nostoc (kind).Another name ground also claims beauty's rice, day rosetangle, " water Auricularia " or " field Auricularia ".The green grain circle of its color, exquisitely carved, profile is quite like Margarita, so have another name called Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis).The very thin algal filament of the diameter number of the not branch that this algae is linked to be by elliptical erythrocyte micron is entwined.All algal filaments all are embedded in the colloid of thick close poly-composition of Salvia polysaccharide, and form spherical colony not of uniform size.In natural environment, Nostoc is common on the small stream stone of river or in the rice terrace, is blackish green spherical, and it is extremely tight to growing environment and conditional request, and worldwide distributed pole is narrow.
Nostoc mainly contains (1) protein and fatty acid: contain 18 seed amino acids, in eight seed amino acids of needed by human, it contains seven kinds, especially the content of lysine and threonine is higher, and kind is more complete, and is rational in infrastructure, meet the mode standard that the FAO/WHO Committee of Experts was recommended in 1973 fully, the dry gross protein is up to about 56%, and it is again a kind of low-fat content food simultaneously, and crude fat content only is 8.11%, take middle carbochain as main, contain unsaturated fatty acid, can reduce the synthetic of body's cholesterol, with prevention and cure of cardiovascular disease, studies show that, lauric acid in the medium-chain fatty acid, tridecanoic acid, myristic acids etc. all have active anticancer in various degree, and to blood pressure lowering, blood fat reducing content has special effect, simultaneously, also can be used as overweight people and glycosuria patient's meals, to help digestion, prevent constipation.
(2) vitamin: Nostoc is rich in multivitamin, carotenoid and phycobilin, and contain chlorophyll 30.98mg/g, and ascorbic acid, Vitamin C content is high, higher more than 5 times than Fructus Crataegi, higher 15 times than mandarin orange, containing abundant vitamin B group is large characteristics of Nostoc, and vitamin B1, B2 are higher than general Homonemeae, the vitamin E that it is abundant makes Nostoc have aging resistance, antioxidation and anticancer function.Vitamin A content is especially high, and every hectogram contains 387 ius, finds for a long time that in history Nostoc is evident in efficacy to ophthalmics such as nyctalopia, conjunctival congestion swelling, and reason namely is this.
(3) trace element: Nostoc contains the important element of the needed by human such as calcium, potassium, ferrum, zinc, strontium, and its calcium content is higher than general plant, is a kind of fabulous Natural Rich Calcium nutraceutical.
(4) polysaccharide: the Nostoc polyoses content is abundant, and up to 20%, the poly-polysaccharose substance that it is abundant by rare in the plant kingdom, ranks first in the plant of reporting at present.
Nostoc has a large amount of historical data to be card as dish and Chinese medicine: beam is for TAO Hong-Jing work " Mingyi Bielu ", thereafter Compendium of Material Medica, " property of medicine is examined ", " national Chinese herbal medicine compilation " etc., the effect of summing up Nostoc is: QI invigorating makes eye bright, made us son, analgesic relieving inflammation or internal heat, sharp the intestines and stomach, phlegm-fire can be treated, for a long time clothes are prolonged life, and allaying tiredness, restrain, control nyctalopia.The yellow front yard of Song dynasty mountain valley Taoist heavily fortified point writes in " green dish money " poem, is described as delicacy from mountain; Ming Dynasty Wang Xin enrolls " wild herbs spectrum ", rear income " agricultural administration book "; The record of Qing Dynasty supplementary Amplifications of the Compendium of Materia Medica that ZHAO Xue-Min is write: " Nostoc is given birth in the Yan Xishan cave, the province of the Yuan Dynasty on the stone, meets heavy rain and washes the cave mouth open; this rice goes out with stream, and natives pull out, when just getting such as little bright Auricularia; be purple green, mixes it with vinegar, and delicious and crisp is edible; the time think thick soup; emerald green lovely just like blue or green spiral shell, the extremely sweet aquatic foods of distinguish the flavor of; sliding crisp and refreshing mouth enters vegetables and is advisable; With water logging it, with meat with boiling, make the Auricularia flavor." " Hefeng was the state originally " record: " and Nostoc, produce over one hundred apart from Zhoucheng (Hefeng was the state originally) in, in the paddy field, all living everywhere outside the Pass the large rock.”
Nostoc is as cuisines during the feast, and its color is dark green lovely, its pure and fresh sweet aquatic foods of distinguishing the flavor of, and vegetal pole horn of plenty not only, but also have the more efficient food therapy value, the Nostoc nature and flavor are sweet, light, cold, and the clearing away heat to improve acuity of vision effect is arranged, and can control conjunctival congestion redness, nyctalopia, scald.According to " property of medicine is examined ": " (Nostoc) is clear refreshing analgesic, and phlegm-fire can be treated, and of a specified duration the clothes prolonged life." " national Chinese herbal medicine compilation " record: Nostoc is cold in nature, lightly seasoned, can heat clearing away, convergence, QI invigorating, make eye bright and can treat nyctalopia, proctoptosis and external and can treat the diseases such as burn, scald.
Summary of the invention
The technical problem to be solved in the present invention is utilize Photobiology and modern technology to prepare nostoc sphaeroids kutz extractive, and take nostoc sphaeroids kutz extractive as functional component, preparation to have the cosmetics of permanent moisturizing water supplement function.
The production of a kind of Nostoc nutrient substance of the present invention and extracting method comprise the steps:
(1) in the Nostoc incubation, on the basis of natural light or daylight lamp irradiation, increase the red-light LED irradiation, the Nostoc polysaccharide molecule is changed, fresh goods Nostoc spherical colony hardness increases, water content reduces to 96% of Nostoc weight, and the Biomass dry weight of fresh goods is increased to 4% of Nostoc weight; (2) special physics is except the color technology: the strong blue light technology of fading, be about to the Nostoc colony high-temperature inactivation of results after, irradiation is 48 hours under strong blue light so that the pigments such as melanin, phycobilin, chlorophyll take off, Nostoc becomes colourless spheroid; (3) colourless Nostoc spheroid filters after homogenate, adds Nostoc weight 2-5 water doubly, boils and extracts three times, filter and collect filtrate, filtrate through be evaporated to add water before the weight of Nostoc; The trichloroacetic acid that adds Nostoc weight 5% stirs, and precipitating proteins removes by filter protein, repeats 3 times and processes; Filtrate becomes paste through concentrating under reduced pressure; The paste drying is ground into Nostoc nourishing and moisturizing extract.
The present invention has following advantage: use this method that the dry of Nostoc is increased, reduced greatly production cost, improved economic benefit; Nostoc nourishing and moisturizing extract has the cosmetology function of nutrition and moisturizing, can be used as the raw material of cosmetics, skin care item, can play to keep moisture of skin and skin nutrition to accelerate the effect of repairing.
The specific embodiment
The following examples can illustrate in greater detail the present invention, but do not limit in any form the present invention.
Embodiment: the cultivation of (1), Nostoc: take by weighing the Nostoc seed of diameter 2mm, put into the Photoreactor that is added with the BG-110 culture fluid, take daylight lamp and LED as light source, total intensity of illumination is 4000lx, and wherein the light intensity of LED and daylight lamp is 2000lx; Illumination every day 16 hours is filled with air 24 hours every days, 24 ℃ of cultivation temperature.Matched group does not add led light source, only with daylight lamp as light source, total intensity of illumination also is 4000lx.Continuous culture was changed one time of nutrition liquid in 10 days.After 40-50 days, the Nostoc diameter reaches more than the 5mm, stops to cultivate.The results Nostoc, elimination moisture.(2), the dry matter content of Nostoc: get 10g Nostoc fresh goods and insert in the air dry oven, under 65 ℃, be dried to constant weight, triplicate.Calculate water content, use Nostoc spheroid water content that present technique (take daylight lamp and LED as light source) obtains as 96.2 ± 0.53%, dry matter content is 3.8%; And matched group (not adding led light source) Nostoc spheroid water content is up to 98.5 ± 1.66%, dry matter content only 1.5%.(3), the high light of Nostoc fades: get A, B, three samples of C, each sample Nostoc fresh weight 500g adds water 1000ml, be heated to boiling 10 minutes, cooling, irradiation is 48 hours under 20000lx high light or the strong blue light of 8000lx, blackish green Nostoc becomes colourless spheroid, and elimination moisture is for subsequent use.(4), Nostoc nourishing and moisturizing extract: with the homogenate 5 minutes in homogenizer of the colourless Nostoc spheroid after the high light fade treatment, add water 1000ml, heated and boiled, reflux, extract, 1 hour, sucking filtration.Filtering residue adds water 1500ml, heated and boiled, reflux, extract, 1 hour, sucking filtration; Repeat again said process once.(5), merging filtrate, be concentrated into 500ml, add 5% trichloroacetic acid, stir, leave standstill under 4 ℃, separate out protein precipitation, remove by filter protein; This processes triplicate.(6), except the extracting solution concentrating under reduced pressure behind the albumen becomes paste, paste is poured in the enamel basin, insert in the drying baker and under 65 ℃, be dried to water content below 7%.Dry thing is pulverized to get Nostoc nourishing and moisturizing extract finished product, and the A sample gets finished product 8.68g, and the B sample gets finished product 9.15g, the C sample gets finished product 8.03g.
One, Nostoc nourishing and moisturizing extract is detected, assay sees Table 1:
Table 1 Nostoc nourishing and moisturizing extract component content
| Title | The A sample | The B sample | The C sample | Meansigma methods |
| Total polysaccharides (%) | 39.78 | 38.80 | 40.02 | 39.53 |
| Crude fat (%) | 7.69 | 6.97 | 7.35 | 7.34 |
| Total protein (%) | 0 | 0 | 0 | 0 |
| Phosphorus P (%) | 1.08 | 0.98 | 0.95 | 1.00 |
| Potassium K (%) | 0.94 | 0.88 | 0.95 | 0.92 |
| Sodium Na (%) | 0.42 | 0.36 | 0.40 | 0.39 |
| Calcium Ca (%) | 3.14 | 2.80 | 3.09 | 3.01 |
| Magnesium Mg (%) | 1.02 | 0.97 | 1.04 | 1.01 |
| Ferrum Fe (%) | 0.92 | 0.74 | 0.87 | 0.84 |
| Zinc Zn (%) | 5.45 | 2.88 | 3.38 | 3.90 |
| Selenium Se (ppm) | 0.24 | 0.20 | 0.20 | 0.21 |
| Chromium Cr (ppm) | 1.17 | 0.75 | 0.92 | 0.95 |
Two, the extraction of Nostoc polysaccharide contrast
Get respectively each 500g of matched group Nostoc fresh goods that LED processes the Nostoc fresh goods and do not adopt LED illumination, homogenate is 5 minutes in homogenizer, adds water 1000ml, heated and boiled, reflux, extract, 1 hour, sucking filtration.Filtering residue adds water 1500ml, heated and boiled, reflux, extract, 1 hour, sucking filtration.Repeat again said process once.
Merging filtrate is concentrated into 1000ml, adds 5% trichloroacetic acid, stirs, and leaves standstill under 4 ℃, separates out protein precipitation, removes by filter protein; This processes triplicate.
Add the concentration of ethanol to 75%, separate out white polysaccharide precipitation; Centrifugal, abandoning supernatant; Add again water, and the heating for dissolving precipitation, filter.Filtrate adds 5% active carbon, and ebuillition of heated 5 minutes removes by filter active carbon, gets water white transparency filtrate.Add again the concentration of ethanol to 75%, separate out polysaccharide precipitation, centrifugal, abandoning supernatant.Taking out polysaccharide dries to constant weight in 65 ℃ of air dry ovens.Get LED and process polysaccharide sample 3.83g, control sample polysaccharide 1.45g.Measure polyoses content with the phenol sulfuric acid process, purity is respectively 94.63% and 96.12%.
Three, the performance of keeping humidity of Nostoc nourishing and moisturizing extract
The present invention estimates the moisture absorption/performance of keeping humidity of Nostoc nourishing and moisturizing extract with the following method:
Accurately take by weighing Nostoc nourishing and moisturizing extract 0.2500g with weighing botle, insert the bottom and fill in the crisper of 100ml water, cover seal cover, ambient temperature is 25-28oC; (moisture absorption 8h, 12h, 24h) weighs behind different time, calculates respectively hydroscopicity by following formula: the weight before hydroscopicity (%)=100 * (weight before the weight-moisture absorption after the moisture absorption)/moisture absorption.
Then the Nostoc nourishing and moisturizing extract after the moisture absorption is changed in the seal preservation box that is placed with dry silica gel, take out behind capable and experienced dry processing 8h, 12h, the 24h and weigh, weight difference according to before and after dry is calculated as follows the moisturizing rate: the weight-dry weight after moisturizing rate (%)=100(dehydration)/(weight-dry weight before the dehydration)
Experimental result sees the following form:
The hydroscopicity of table 2 Nostoc nourishing and moisturizing extract and moisturizing rate experimental result
As known from Table 2, although the content of polysaccharide is less than half of polysaccharide sample in the Nostoc nourishing and moisturizing extract, its moistening effect only slightly descends than holosaccharide.Water absorption rate and the moisturizing rate of the Nostoc polysaccharide of Nostoc nourishing and moisturizing extract and two kinds of method acquisitions are all very high.
Four, use the product of moisturizing extract
Nostoc moisture saver mask preparation: A phase: Arlacel-60 0.82%, hexadecanol 1.68%, vaseline 0.68%, dimethicone 2.03%, squalane 2.33%, NPGC-2 0.84%, elastic silica gel 0.17%.B phase: Tween-60 1.26%; 1,3 butylene glycol 1.68%, glycerol 12.76%; Acritamer 940 0.43%; Nostoc sphaeroids kutz extractive 10.32%; Nertralizer: TEA 0.43%; Deionized water 64.10%.C phase: extremely U.S. II 0.30%; Essence 0.10%.
Preparation technology is as follows: (1) adds container A successively with each component of A phase, is heated to 75 ℃, stirs; (2) each component of B phase is added container B successively, be heated to 75 ℃, stir; (3) while hot the solution of step 1 is poured in the solution of step 2 into 5000rpm homogenizing 3 minutes; (4) when temperature is down to 40 ℃, add the C phase, stir; (5) naturally cool to room temperature, cold preservation is for subsequent use.
The preparation of Nostoc moisturizing essence: A phase: Montanov 68 2.05%, dimethicone 0.68%, squalane 6.5%, vitamin E 0.68%, TT 0.05%.B phase: glycerol 6.85%, 1,3 butylene glycol 4.56%, HANSHENGJIAO 0.27%, EDTA-Na2 0.04%, S-165 2.05%, nostoc sphaeroids kutz extractive 19.67%, allantoin 0.10%, deionized water 55.26%.C phase: Geogard Ultra 1.14%.D phase: essence: DHT-4080 0.10%.
Preparation technology is as follows: (1) is got C and is dissolved in mutually in the B phase water, and the slight fever stirring and dissolving is (speed is slightly slow) evenly, and is for subsequent use; (2) with A mutually each component add in container A, heating is stirred to whole dissolvings, control temperature to 75 ℃; (3) each component that dewaters mutually the impregnating fluid of step 1 and B outer adds in container B, and heating is stirred to whole dissolvings, control temperature to 75 ℃; (4) under constantly stirring, the solution with step 2 slowly adds in the mixed liquor of step 3 while hot, and fast homogeneous stirred 2-3 minute, made into emulsion; (5) add D phase essence, stir; (6) naturally cool to room temperature, cold preservation is for subsequent use.
Claims (1)
1. the production of Nostoc nutrient substance and extracting method is characterized in that, step is as follows:
(1), the cultivation of Nostoc: take by weighing the Nostoc seed of diameter 2mm, put into the Photoreactor that is added with the BG-110 culture fluid, take daylight lamp and LED as light source, total intensity of illumination is 4000lx, and wherein the light intensity of LED and daylight lamp is 2000lx; Illumination every day 16 hours is filled with air 24 hours every days, 24 ℃ of cultivation temperature; Continuous culture was changed one time of nutrition liquid in 10 days; After 40-50 days, the Nostoc diameter reaches more than the 5mm, stops to cultivate; The results Nostoc, elimination moisture;
(2), the dry matter content of Nostoc: get 10g Nostoc fresh goods and insert in the air dry oven, under 65 ℃, be dried to constant weight, triplicate; Calculate water content, the Nostoc spheroid water content of acquisition is 96.2 ± 0.53%, and dry matter content is 3.8%;
(3), the high light of Nostoc fades: get A, B, three samples of C, each sample Nostoc fresh weight 500g adds water 1000ml, be heated to boiling 10 minutes, cooling, irradiation is 48 hours under 20000lx high light or the strong blue light of 8000lx, blackish green Nostoc becomes colourless spheroid, and elimination moisture is for subsequent use;
(4), Nostoc nourishing and moisturizing extract: with the homogenate 5 minutes in homogenizer of the colourless Nostoc spheroid after the high light fade treatment, add water 1000ml, heated and boiled, reflux, extract, 1 hour, sucking filtration; Filtering residue adds water 1500ml, heated and boiled, reflux, extract, 1 hour, sucking filtration; Repeat again said process once;
(5), merging filtrate, be concentrated into 500ml, add 5% trichloroacetic acid, stir, leave standstill under 4 ℃, separate out protein precipitation, remove by filter protein; This processes triplicate;
(6), except the extracting solution concentrating under reduced pressure behind the albumen becomes paste, paste is poured in the enamel basin, insert in the drying baker and under 65 ℃, be dried to water content below 7%; Dry thing is pulverized to get Nostoc nourishing and moisturizing extract finished product, and the A sample gets finished product 8.68g, and the B sample gets finished product 9.15g, the C sample gets finished product 8.03g.
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| CN104958247B (en) * | 2015-06-30 | 2018-02-09 | 湖南炎帝生物工程有限公司 | A kind of body lotion containing nostoc sphaeroids kutz extractive and preparation method thereof |
| CN105796427B (en) * | 2016-04-13 | 2018-07-03 | 梁春梅 | A kind of preparation method of ice plant extract |
| CN106539739B (en) * | 2016-09-29 | 2020-01-17 | 常德炎帝生物科技有限公司 | Nostoc sphaeroids kutz cotton mask and preparation method and application thereof |
| CN106309284B (en) * | 2016-09-29 | 2019-09-20 | 常德炎帝生物科技有限公司 | A kind of nostoc cotton pads and the preparation method and application thereof |
| CN106344420B (en) * | 2016-09-29 | 2019-01-29 | 常德炎帝生物科技有限公司 | A kind of nostoc Multifunctional cotton and the preparation method and application thereof |
| CN109511951A (en) * | 2017-09-20 | 2019-03-26 | 广安职业技术学院 | A kind of tasty and refreshing land for building dish and preparation method thereof |
| CN108611304B (en) * | 2018-05-16 | 2020-09-25 | 常德炎帝生物科技有限公司 | Ecological simulation preservation method for nostoc sphaeroides |
| CN110403200A (en) * | 2018-08-07 | 2019-11-05 | 湖南炎帝生物工程有限公司 | Nostoc composition and its application in alleviation eye strain, improvement eye maculopathy |
| CN114224819B (en) * | 2021-12-27 | 2023-04-25 | 湖南炎帝生物工程有限公司 | Nostoc sphaeroids kutz homogenate extracting solution and preparation method and application thereof |
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