CN106432480A - Extraction method of phycobiliprotein of nostoc sphaeroides kutzing - Google Patents
Extraction method of phycobiliprotein of nostoc sphaeroides kutzing Download PDFInfo
- Publication number
- CN106432480A CN106432480A CN201610807802.2A CN201610807802A CN106432480A CN 106432480 A CN106432480 A CN 106432480A CN 201610807802 A CN201610807802 A CN 201610807802A CN 106432480 A CN106432480 A CN 106432480A
- Authority
- CN
- China
- Prior art keywords
- nostoc
- phycobiliprotein
- phycobniliprotein
- kutzing
- mesh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an extraction method of phycobiliprotein of nostoc sphaeroides kutzing. The extraction method comprises the following steps of crushing dry nostoc sphaeroides kutzing to be 20 to 80 meshes, adding purified water of which the weight is 40 to 60 times the weight of the crushed dry nostoc sphaeroides kutzing to extract the phycobiliprotein for 4h to 8h, filtering an obtained mixture, and carrying out freeze drying on a filtrate, so that the phycobiliprotein is obtained. According to the method provided by the invention, the phycobiliprotein is directly extracted by using 20 to 80 meshes of nostoc sphaeroides kutzing dry powder; the extraction process is simplified; compared with a method for extracting the phycobiliprotein by adopting multigelation, ultrasonic wall breaking, soaking by a phosphate buffer solution and salting out by ammonium sulfate, the extraction method is simple and is easy to operate; further, the yield of a phycobiliprotein crude extract of the nostoc sphaeroides kutzing is improved; the content of the crude extract phycobiliprotein is improved. By using the method provided by the invention, a simpler, more practical, cost-saving and safe technical process which is capable for industrialized mass production is provided for a production enterprise.
Description
Technical field
The present invention relates to a kind of method that convenient and efficient ground slightly carries Nostoc phycobniliprotein.
Background technology
Nostoc scientific name intends spherical nostoc (Nostoc sphaeroides Kutzing), also known as beauty's rice, sky rosetangle,
It is a kind of edible Azotica, be a kind of groups state algae.Its frond contains rich in protein, 8 kinds of needed by human body ammonia
Base acid, algae starch, Folic Acid, soluble fiber, multivitamin and 15 kinds of mineral, the abundantest has phosphorus, sulfur, calcium, potassium, ferrum
Deng trace element has zinc, copper, manganese, selenium, and the height of its calcium content is extremely rare in vegetable, is that a kind of high-calcium low-fat fat nutrition is equal
The food of weighing apparatus.According to《Compendium of Materia Medica》With《A Supplement to the Compendium of Materia Medica》Deng record, Nostoc can treat nyctalopia, proctoptosis, burn and scald etc..
Additionally, according to《National Chinese herbal medicine compilation》Introduce, Nostoc is cold in nature, lightly seasoned, has the medicinal healths such as heat clearing away, convergence, QI invigorating, improving eyesight
Effect.Nostoc has considerable edible, medical value and health care in sum.
Phycobniliprotein is that algae is distinctive catches photochromic fibroin, is a kind of important bioactive substance.Known algae gallbladder egg
Mainly comprise phycoerythrin (PE), phycocyanin (PC), phycoerythrocyanin (pec) (PEC), allophycocyanin (APC) in vain, it can conduct
Natural food colour, not only color is beautiful, and has radioprotective, antiinflammatory, promote wound healing, adjust immunity, promotion
The effects such as hemopoietic, anticancer.Phycobniliprotein is used for cosmetics, dyestuff, clinical diagnose and immunochemistry and biological engineering at present
Etc. in research field, food can also be made and medicine is used for health care.
There are some researches show that the phycobniliprotein in Nostoc comprises chromoprotein species entirely (PC, PE, PEC and APC), wherein algae
At most, this point is better than other unicellular algas and colony for Lactoferrin (PE), phycocyanin (PC) and phycoerythrocyanin (pec) (PEC) content
State algae, such as delivers vegetables, spirulina and Thallus Porphyrae etc..Therefore extracting phycobniliprotein with Nostoc is most to develop in algae
It is worth the research with potentiality.
Repeatedly frozen using Nostoc dry powder fine powder (100 mesh) low temperature more than the existing technology extracted for Nostoc phycobniliprotein
Melt, ultrasonication, the method for phosphate buffer extraction.Although this method can extract phycobniliprotein, yield compares
Low, only 7% about, and with the addition of organic reagent during using it is impossible to ensure the safety of final finished；Conventional extraction
Method carries out research in extraction process and uses instrument and equipment more, and complex operation does not consider in real enterprise production process
In can consume a large amount of human and material resources and energy consumption；How and great majority research all concentrates on phycocyanin from Nostoc algae gallbladder
Extract in albumen and separate, the phycobniliprotein without considering to be rich in multiple nutrient substance has more edibility.In consideration of it, having
The necessary a kind of method extracting phycobniliprotein from Nostoc developing quickness and high efficiency is beneficial to the big production of enterprise.
Content of the invention
It is an object of the invention to provide a kind of method that quickness and high efficiency ground slightly carries Nostoc phycobniliprotein.
For achieving the above object, the present invention provides a kind of extracting method of Nostoc phycobniliprotein, including the Pueraria lobota that will be dried
Celestial rice flour is broken to 20-80 mesh, adds the purified water extraction 4-8h of 40-60 times of weight, filters, and slightly carrying rich in phycobniliprotein is obtained
Liquid, lyophilization, obtain final product Nostoc phycobniliprotein crude extract.
Preferably, described Nostoc particle diameter is 60-80 mesh, more preferably 60 mesh or 80 mesh；Preferably 60 mesh.
Preferably, described Nostoc and the weight ratio of purified water are 1:50.
Preferably, described extraction time is 6h.
Preferably, described extraction is carried out at room temperature, general 20～25 DEG C.
Preferably, described Nostoc water content is 7%-13%, more preferably 11%.
Preferably, also include the step being centrifuged before described filtration, described centrifugal rotational speed is 5000-10000r/min, centrifugation
Time is 15-30min；It is further preferred that described centrifugal rotational speed is 8000r/min, centrifugation time is 15min.
Specifically, the extracting method of above-mentioned Nostoc phycobniliprotein, including the Nostoc being 11% to water content by drying
It is crushed to 60-80 mesh, add the purified water room temperature of 40-60 times of weight to extract 6h, be centrifuged under the conditions of rotating speed 8000r/min
15min, filters, and crude extract rich in phycobniliprotein, lyophilization is obtained, obtains final product Nostoc phycobniliprotein crude extract.
Compared with prior art, the present invention has advantages below：
1. the inventive method uses the Nostoc dry powder extracting directly phycobniliprotein of 20-80 mesh, fills up this research field
Blank；The inventive method simplifies extraction process, than using multigelation, ultrasonication, phosphate buffer immersion, sulphuric acid
The method that phycobniliprotein is extracted in ammonium salt analysis is simple to operation；
2. the inventive method improves the yield of Nostoc phycobniliprotein crude extract, higher by one than existing freeze-thaw method extracts yield
Times many, and also improve the Phycobiliprotein Content of crude extract, bring up to 12% from 7%；
3. the inventive method does not need using any organic reagent, does not destroy multiple important pigment eggs in phycobniliprotein
In vain, it really is able to accomplish safety zero interpolation, trust edible；
4. this method has also been innovated on Extracting temperature, and realizing extraction phycobniliprotein need not be carried out under cryogenic,
This breaches the restriction to Extracting temperature for the method commonly used at present；
5. the inventive method mainly adopts Nostoc dry, and it is easy to store than fresh Nostoc, is using, is transporting
During convenient, cost-effective, it is also high than phycobniliprotein yield after the pulverizing of fresh Nostoc simultaneously；There is provided one for manufacturing enterprise
Bar more simple and practical, cost-effective, safe can industrialized great production technical matters.
Specific embodiment
Following examples are used for the present invention is described, but are not limited to the scope of the present invention.Unreceipted concrete in embodiment
Technology or condition person, according to the technology described by document in the art or condition, or are carried out according to product description.Used
Reagent or the unreceipted production firm person of instrument, are the conventional products being commercially available by regular distributor.
Following Phycobiliprotein Content detection method be UV-VIS spectrophotometry, measure wavelength 562nm, 615nm,
Light absorption value at 652nm, by document " Bennett A, Bogorad L.Complementary chromatic adaptation
In a filamentous blue-green-alga [J] .Journal of Cell Biology, 1973,58 (2)：419—
435 " method recorded calculates content.
The computational methods of described below yield are：
Yield %={ phycobniliprotein crude extract weight/Nostoc coarse powder weight × (1- moisture) } × 100
Embodiment 1 carries out laboratory lab scale using the inventive method and slightly carries phycobniliprotein
Particle diameter will be crushed to through drying or drying moisture Nostoc dry (water content 11%) with Universalpulverizer
60 mesh, take this Nostoc coarse powder 21.99g, 23.94g, 23.35g to be placed in the purified water of addition 1100g in beaker, under room temperature respectively
After 6 hours, 8000r/min is centrifuged 15min to standing, filters, obtains crude extract, crude extract lyophilization is obtained Nostoc algae gallbladder
Protein crude extract administration.Result see table 1：
Table 1
From upper table 1, extract that to obtain crude extract yield higher for three times, all have more than 20%, meansigma methodss are 22.5%, Pueraria lobota
In celestial rice phycobniliprotein crude extract, Phycobiliprotein Content meansigma methodss are 12.5%.Repeat experimental verifications side of the present invention through three times
Method has good repeatability.
Embodiment 2 carries out laboratory lab scale using the inventive method and slightly carries phycobniliprotein
Particle diameter will be crushed to through drying or drying moisture Nostoc dry (water content 11%) with Universalpulverizer
80 mesh, take this Nostoc coarse powder 25.12g, 25.30g, 25.60g to be placed in the purified water of addition 1260g in beaker, under room temperature respectively
After 6 hours, 8000r/min is centrifuged 15min to standing, filters, obtains crude extract, crude extract lyophilization is obtained Nostoc algae gallbladder
Protein crude extract administration.Result see table 2：
Table 2
From upper table 2, extract that to obtain crude extract yield higher for three times, all have more than 10%, meansigma methodss are 11.1%, Pueraria lobota
In celestial rice phycobniliprotein crude extract, Phycobiliprotein Content meansigma methodss are 10.1%.Repeat experimental verifications side of the present invention through three times
Method has good repeatability.
Embodiment 3 carries out laboratory lab scale using the inventive method and slightly carries phycobniliprotein
Particle diameter will be crushed to through drying or drying moisture Nostoc dry (water content 11%) with Universalpulverizer
20 mesh, take this Nostoc coarse powder 24.87g, 25.45g, 25.00g to be placed in the purified water of addition 1260g in beaker, under room temperature respectively
After 6 hours, 8000r/min is centrifuged 15min to standing, filters, obtains crude extract, crude extract lyophilization is obtained Nostoc algae gallbladder
Protein crude extract administration.Result see table 3：
Table 3
From upper table 3, extract that to obtain crude extract yield higher for three times, all have more than 10%, meansigma methodss are 13.2%, Pueraria lobota
In celestial rice phycobniliprotein crude extract, Phycobiliprotein Content meansigma methodss are 9.8%.Repeat experimental verifications side of the present invention through three times
Method has good repeatability.
Comparative example 1
Nostoc dry (water content 11%) is crushed to particle diameter 100 mesh, take this Nostoc fine powder 12.92g,
12.39g, 13.51g, the method recorded by document " Wang Xing equality, application on Nostoc wall breaking technology for the multigelation method ",
Nostoc phycobniliprotein, prepared Nostoc phycobniliprotein crude extract are extracted using thawing method.Result see table 4：
Table 4
From upper table 4, extracting the crude extract yield meansigma methodss obtaining for three times is 16.3%, and Nostoc phycobniliprotein slightly carries
In thing, Phycobiliprotein Content meansigma methodss are 7.1%, and in Nostoc phycobniliprotein crude extract, Phycobiliprotein Content is all substantially less than in fact
Apply example 1.
Multigelation not only increases number of operations, and can increase cost in production application, and side of the present invention
Method once-through operation, it is workable to demonstrate this law, easy and simple to handle, and use cost is far below multigelation.
Comparative example 2
Take fresh Nostoc (water content 98%) 1.78kg, 1.74kg, 1.80kg respectively, by with comparative example 1 identical side
Method extracts Nostoc phycobniliprotein, prepared Nostoc phycobniliprotein crude extract.Result see table 5：
Table 5
From upper table 5, three times freeze-thaw method extracts yield all below 10%, and meansigma methodss are 7.5%, Nostoc algae gallbladder egg
In white crude extract, Phycobiliprotein Content meansigma methodss are 2.2%.From table 1 and table 5, extracted from dry powder using the inventive method
Phycobniliprotein crude extract is higher more than 3 times than obtain from fresh Nostoc.This is also this method from the preparation of Nostoc dry product
The reason without fresh Nostoc.
Comparative example 3
Take Nostoc coarse powder (water content 11%) 33.88g, 33.49g, the 22.21g being crushed to particle diameter in 60 mesh respectively, adopt
Extract phycobniliprotein, prepared Nostoc phycobniliprotein crude extract with comparative example 1 multigelation method, result see table 6：
Table 6
From upper table 6, extract yield for three times all more than 10%, the crude extract yield meansigma methodss obtaining are 13.4%；
In Nostoc phycobniliprotein crude extract, Phycobiliprotein Content meansigma methodss are 5.5%.From table 1 and table 6, Nostoc phycobniliprotein
In crude extract, Phycobiliprotein Content is all substantially less than embodiment 1.
In addition, this comparative example 3 gained Nostoc phycobniliprotein crude extract finished appearance, color are all be not as good as embodiment 1.Real
Applying example 1 gained Nostoc phycobniliprotein crude extract color is purple, and comparative example 3 gained Nostoc phycobniliprotein crude extract color
For purplish grey.
The algae that the inventive method obtains than the thawing method extraction commonly using at present is demonstrated again by contrast
Biliprotein crude extract yield is high, and in Nostoc phycobniliprotein crude extract, Phycobiliprotein Content is high, demonstrates the high efficiency of this law.
Comparative example 4
Nostoc dry (water content 11%) is taken to be crushed to particle diameter in 10 mesh, 60 mesh, each 22.47g of 100 mesh respectively, point
Do not extracted using embodiment 1 method and comparative example 1 freeze-thaw method, prepared Nostoc phycobniliprotein crude extract.Its content is entered
Row contrast, result see table 7：
Table 7
From upper table 7, particle diameter excessive (10 mesh) Nostoc dry fails to pulverize completely it is impossible to extract phycobniliprotein；
Fine powder (100 mesh) content is extracted using embodiment 1 method and yield is not all high；Embodiment 1 method coarse powder (60 mesh) is extracted and is obtained
Crude extract Phycobiliprotein Content be higher than comparative example 1 fine powder (100 mesh) freeze-thaw method.
In sum, Phycobiliprotein Content can be obtained more than 10% from Nostoc using the inventive method, extract
Crude extract more than 20% for the yield, and the inventive method than in the research of existing report adopt preparation technology cycle is short,
Easy and simple to handle；Higher than using fresh Nostoc and Nostoc fine powder extraction yield, phycobniliprotein is also contained by the inventive method simultaneously
Measure report from existing document 7% brings up to more than 12%.Therefore the inventive method has obvious advantage than other extraction processes
And innovation.
Although, above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. a kind of extracting method of Nostoc phycobniliprotein is it is characterised in that include for the Nostoc being dried being crushed to 20-80
Mesh, adds the purified water extraction 4-8h of 40-60 times of weight, filters, lyophilization, obtains final product.
2. method according to claim 1 it is characterised in that described Nostoc particle diameter be 60-80 mesh, preferably 60 mesh or
80 mesh.
3. method according to claim 1 and 2 is it is characterised in that described Nostoc is 1 with the weight ratio of purified water:50.
4. method according to claim 1 and 2 is it is characterised in that described extraction time is 6h.
5. method according to claim 1 and 2 is it is characterised in that described extraction is carried out at room temperature.
6. method according to claim 1 and 2 is it is characterised in that described Nostoc water content is 7%-13%；Preferably contain
The water yield is 11%.
7. method according to claim 1 and 2 it is characterised in that before described filtration also include be centrifuged step, described from
Heart rotating speed is 5000-10000r/min, and centrifugation time is 15-30min.
8. it is characterised in that described centrifugal rotational speed is 8000r/min, centrifugation time is method according to claim 7
15min.
9. method according to claim 1 is it is characterised in that include pulverizing the Nostoc being 11% to water content is dried
To 60-80 mesh, add the purified water room temperature of 40-60 times of weight to extract 6h, be centrifuged 15min, mistake under the conditions of rotating speed 8000r/min
Filter, lyophilization, obtain final product.
10. the Nostoc phycobniliprotein that any one of claim 1-9 methods described is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610807802.2A CN106432480A (en) | 2016-09-07 | 2016-09-07 | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610807802.2A CN106432480A (en) | 2016-09-07 | 2016-09-07 | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106432480A true CN106432480A (en) | 2017-02-22 |
Family
ID=58164218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610807802.2A Pending CN106432480A (en) | 2016-09-07 | 2016-09-07 | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106432480A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110403200A (en) * | 2018-08-07 | 2019-11-05 | 湖南炎帝生物工程有限公司 | Nostoc composition and its application in alleviation eye strain, improvement eye maculopathy |
| CN113025676A (en) * | 2021-03-12 | 2021-06-25 | 云南孤狼翼族生物科技有限公司 | Pure natural compound amino acid small molecule active peptide extracted from plants, and extraction method, preparation and application thereof |
| CN113292648A (en) * | 2021-07-02 | 2021-08-24 | 华东理工大学 | Method for separating and purifying high-purity phycobiliprotein from nitrogen-fixing blue algae and phycobiliprotein |
| CN114702557A (en) * | 2021-07-09 | 2022-07-05 | 杭州职业技术学院 | Preparation method of nostoc sphaeroides hemoglobin |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822630A (en) * | 2010-03-24 | 2010-09-08 | 湖南炎帝生物工程有限公司 | Nostoc sphaeroids kutz extractive and application thereof in cosmetics |
| CN102440329A (en) * | 2010-10-14 | 2012-05-09 | 吴乐秦 | Preparation technology of porphyra haitanensis phycobiliprotein powder |
| CN102871133A (en) * | 2011-07-13 | 2013-01-16 | 祁婉芸 | Preparation technology for laver phycobiliprotein powder |
| CN105254774A (en) * | 2015-11-09 | 2016-01-20 | 湖南炎帝生物工程有限公司 | Nostoc commune polysaccharide extraction method |
-
2016
- 2016-09-07 CN CN201610807802.2A patent/CN106432480A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822630A (en) * | 2010-03-24 | 2010-09-08 | 湖南炎帝生物工程有限公司 | Nostoc sphaeroids kutz extractive and application thereof in cosmetics |
| CN102440329A (en) * | 2010-10-14 | 2012-05-09 | 吴乐秦 | Preparation technology of porphyra haitanensis phycobiliprotein powder |
| CN102871133A (en) * | 2011-07-13 | 2013-01-16 | 祁婉芸 | Preparation technology for laver phycobiliprotein powder |
| CN105254774A (en) * | 2015-11-09 | 2016-01-20 | 湖南炎帝生物工程有限公司 | Nostoc commune polysaccharide extraction method |
Non-Patent Citations (1)
| Title |
|---|
| 陈德文: "葛仙米藻红蛋白的分离、纯化与结构及生物活性研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110403200A (en) * | 2018-08-07 | 2019-11-05 | 湖南炎帝生物工程有限公司 | Nostoc composition and its application in alleviation eye strain, improvement eye maculopathy |
| CN113025676A (en) * | 2021-03-12 | 2021-06-25 | 云南孤狼翼族生物科技有限公司 | Pure natural compound amino acid small molecule active peptide extracted from plants, and extraction method, preparation and application thereof |
| CN113292648A (en) * | 2021-07-02 | 2021-08-24 | 华东理工大学 | Method for separating and purifying high-purity phycobiliprotein from nitrogen-fixing blue algae and phycobiliprotein |
| CN113292648B (en) * | 2021-07-02 | 2024-02-13 | 南通聚科生物科技有限公司 | Method for separating and purifying high-purity phycobiliprotein from nitrogen-fixing blue algae and phycobiliprotein |
| CN114702557A (en) * | 2021-07-09 | 2022-07-05 | 杭州职业技术学院 | Preparation method of nostoc sphaeroides hemoglobin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102302427B (en) | Production and extraction method of nostoc nourishing and moisturizing material | |
| CN101828744B (en) | Anti-fatigue total nutrition high activity seacucumber drinking liquid capable of improving immunity and preparation method thereof | |
| CN103141895B (en) | Natural marine animal peptide beautifying drink | |
| CN106432480A (en) | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing | |
| CN101978841A (en) | Protein allergy-resisting and nutritious infant formula milk powder | |
| CN104432318A (en) | Preparation method of chlorella beverage | |
| CN103082291A (en) | Industrial application technology of lycium ruthenicum fruit | |
| CN103392827B (en) | Moringa leaf preserved bean curd | |
| KR20020024129A (en) | Alcohol containing substances that become medicine | |
| CN103519035A (en) | Preparation method of chlorella fermented jelly | |
| CN102631662B (en) | Composition with functions of protecting liver and relieving fatigue and preparation method of composition | |
| CN105533740A (en) | Sea cucumber-traditional Chinese medicine extract with kidney-invigorating and essence-replenishing functions as well as preparation technology thereof | |
| KR20020024127A (en) | Drinks containing substances that become medicine | |
| CN104095897A (en) | Maca dunaliella salina compound preparation | |
| CN103349155B (en) | A kind of yellow eel nutrient fodder | |
| CN106176818A (en) | A kind of earthworm extract and its production and use | |
| CN103960692A (en) | Preparation method of specially-made sea cucumber powder | |
| CN103947857A (en) | Palatable sweet pig forage and preparation method thereof | |
| CN104126634B (en) | One is delivered vegetables biscuit and preparation method thereof | |
| CN106213130A (en) | Bacterium algae compound nutritional rice flour and production method thereof is kept fit without the additive-free brain-strengthening of sensitization | |
| CN105942124B (en) | A kind of beautifying face and moistering lotion solid beverage and preparation method thereof | |
| KR20030064430A (en) | Soy with substances that become medicine | |
| CN107593617A (en) | A kind of method for breeding for imitating wild Polyhachis vicina Roger | |
| CN104663585B (en) | Food toad return of spring worm breeding method and obtained pharmaceutical raw material | |
| CN107802726A (en) | A kind of raspberry health product and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170222 |
|
| RJ01 | Rejection of invention patent application after publication |