CN102871133A - Preparation technology for laver phycobiliprotein powder - Google Patents
Preparation technology for laver phycobiliprotein powder Download PDFInfo
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- CN102871133A CN102871133A CN2011101947908A CN201110194790A CN102871133A CN 102871133 A CN102871133 A CN 102871133A CN 2011101947908 A CN2011101947908 A CN 2011101947908A CN 201110194790 A CN201110194790 A CN 201110194790A CN 102871133 A CN102871133 A CN 102871133A
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- phycobniliprotein
- laver
- powder
- agar
- phycobiliprotein
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Abstract
The invention belongs to the nutriment processing field, and more specifically relates to a preparation technology for laver phycobiliprotein powder. The preparation technology which is simple, effective and suitable for large-scale production for the laver phycobiliprotein powder is provided by the invention. The preparation technology comprises the steps of a laver cell disruption process, preparation of crude extracts of the phycobiliprotein and purification of the phycobiliprotein.
Description
Technical field:
The invention belongs to the manufacture field of nutriment, more particularly, relate to the preparation technology of agar-agar phycobiliprotein powder.
Background technology:
Laver contains special chromoprotein---phycobniliprotein (phycobiliproteins, PBP), this is that a class is present in the important photosynthetic pigments albumen composition in red algae, blue-green alge and the hidden algae, it can pass chlorophyll the luminous energy efficient transmission of catching, thereby the photosynthesis of marine alga is occured.The content of phycobniliprotein in algae can reach the 25%-28% of dry cell weight, it comprises phycoerythrin (phycoerythrin, PE), phycocyanin (phycocyanin, PC), allophycocyanin (allo-phycocyanin, APC) and phycoerythrocyanin (pec) (phycoery-throcyanin).Phycobniliprotein is a kind of raw material of very important photo-dynamical medicine, but distinctive light sensitive effect auxiliary laser is for cancer, in the lesions position enrichment, and efficient generation free radical and active attitude oxygen behind the absorption light, thereby realization killing and wounding tumour cell.Phototoxicity is arranged and do not have dark toxicity, fast the human body metabolism, phycobniliprotein also has oxidation and removing free radicals and improves the effect of immunity.The immunofluorescence label antibody of its making, detection sensitivity is higher than traditional fluorescent marker far away, can be used for particular molecule location and important molecule and detect, comprise the immune molecule detection of SARS virus, exactly diagnosing tumour, determine diseased region and severe extent etc.Phycobniliprotein can be used as function factor and is used for health food and functional food and special feed, and while or desirable natural colouring matter can be used for food industry and cosmetics industry etc. in a large number, have no side effect, and be safe additive.In addition, current research finds that it also has potential application prospect at aspects such as optical information storage and processing, Quick photoelectric detection, artificial neural networks.But because the difficulty of phycobniliprotein separation and purification, existing phycobniliprotein commodity price is expensive, makes its application be subject to certain restriction.Although there are many people to attempt phycobiliprotein gene is changed in the microorganism to realize the large-scale production of phycobniliprotein, also have very long stretch to walk but produce phycobniliprotein with transgenic method, from marine alga, extract at present the separation phycobniliprotein and be still the main path that obtains phycobniliprotein.
China is also very little to dynamics and the degree of depth of algae development and use, and major part only limits to simple edible, and exported product also is only limited to early-products, does not also use high-tech and carries out profound processing and utilization.Some high-tech deep processed products of algae then mainly rely on import, and are very expensive as the drug use price.The at present production of phycoerythrin is substantially by U.S. Sigma company monopolizing, and domestic have with Bangiales, purple ball algae and the spirulina report as raw material extraction phycoerythrin, still is at present conceptual phase, do not have finished product to come into the market to sell.China marine site is broad, and algae resource is very abundant, is maximum in the world laver big producing country, and it is coastal that China's laver culture and processing mainly concentrate on again Soviet Union, Zhejiang, Fujian, and its output accounts for more than 95% of the whole nation.The market price that Rudong County, Jiangsu Province Porphyra yezoensis output accounts for 50%. lavers of national total amount is 1/10 of Bangiales, 1/3 of chlorella and spirulina.Select laver to extract phycobniliprotein, have a cost low, the advantage that yield is high.Its major part of phycobniliprotein in the laver mainly is phycoerythrin (PE).Because the phycobniliprotein isolation and purification method reported is complicated, cause that production cost is too high can't to prepare to satisfy the market potential demand in a large number.
Summary of the invention:
The present invention is exactly for the problems referred to above, and a kind of preparation technology of simple and effective, suitable mass-produced agar-agar phycobiliprotein powder is provided.
For realizing above-mentioned purpose of the present invention, processing step of the present invention is:
1. laver cell crushing process
With pulverizer laver is pulverized first, the laver powder after then will pulverizing is put into water and was soaked 5～10 hours, takes out soak, pulverizes with ultrasonic wave.
2. the preparation of phycobniliprotein crude extract
Directly soak the frond cell with distilled water or low salting liquid, make its swelling, break, discharge phycobniliprotein.Distilled water immersion takes 10 days, and low salting liquid soaks and takes 3～4 days.
Take by weighing the 5.99g laver powder, use distilled water immersion, fully swelling.After 12 days, with 74 microns filter-cloth filtering, and with filtrate at 4 ℃ of centrifugal 20min of lower 5500g, remove the frond residue, get the aubergine supernatant and be the phycobniliprotein crude extract.
3. the purifying of phycobniliprotein
The crude extract of phycobniliprotein is joined the experiment of saltouing of the saturated sulfate of ammoniac solution of 15%～45% gradient, its saturated sulfate of ammoniac solution gradient arranges following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 ℃ are spent the night, and at 4 ℃ of lower 20000g refrigerated centrifuge 15min, get precipitation, and precipitation is dissolved in the phosphate buffer (pH 6.8) of low concentration, survey its light absorption value, in order to carrying out next step purifying work.
4. the concentrate drying of phycobniliprotein solution
Phycobniliprotein behind the above-mentioned purifying steamed to material with rotation vacuum evaporation instrument be aubergine glue (temperature control is in 40 ℃).
The quality that obtains respectively phycoerythrin after the drying is 0.643g, phycocyanin 0.452g.
The specific embodiment:
The present invention prepares the agar-agar phycobiliprotein powder take laver as raw material, and the preparation process of this invention is:
1. laver cell crushing process
With pulverizer laver is pulverized first, the laver powder after then will pulverizing is put into water and was soaked 5～10 hours, takes out soak, pulverizes with ultrasonic wave.
2. the preparation of phycobniliprotein crude extract
Directly soak the frond cell with distilled water or low salting liquid, make its swelling, break, discharge phycobniliprotein.Distilled water immersion takes 10 days, and low salting liquid soaks and takes 3～4 days.
Take by weighing the 5.99g laver powder, use distilled water immersion, fully swelling.After 12 days, with 74 microns filter-cloth filtering, and with filtrate at 4 ℃ of centrifugal 20min of lower 5500g, remove the frond residue, get the aubergine supernatant and be the phycobniliprotein crude extract.
3. the purifying of phycobniliprotein
The crude extract of phycobniliprotein is joined the experiment of saltouing of the saturated sulfate of ammoniac solution of 15%～45% gradient, its saturated sulfate of ammoniac solution gradient arranges following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 ℃ are spent the night, and at 4 ℃ of lower 20000g refrigerated centrifuge 15min, get precipitation, and precipitation is dissolved in the phosphate buffer (pH 6.8) of low concentration, survey its light absorption value, in order to carrying out next step purifying work.
4. the concentrate drying of phycobniliprotein solution
Phycobniliprotein behind the above-mentioned purifying steamed to material with rotation vacuum evaporation instrument be aubergine glue (temperature control is in 40 ℃).
The quality that obtains respectively phycoerythrin after the drying is 0.643g, phycocyanin 0.452g.
Claims (1)
1. the preparation technology of agar-agar phycobiliprotein powder prepares the agar-agar phycobiliprotein powder take laver as raw material, it is characterized in that preparation process of the present invention is:
(1) laver cell crushing process
With pulverizer laver is pulverized first, the laver powder after then will pulverizing is put into water and was soaked 5～10 hours, takes out soak, pulverizes with ultrasonic wave;
(2) preparation of phycobniliprotein crude extract
Directly soak the frond cell with distilled water or low salting liquid, make its swelling, break, discharge phycobniliprotein.Distilled water immersion takes 10 days, and low salting liquid soaks and takes 3～4 days;
Take by weighing the 5.99g laver powder, use distilled water immersion, fully swelling.After 12 days, with 74 microns filter-cloth filtering, and with filtrate at 4 ℃ of centrifugal 20min of lower 5500g, remove the frond residue, get the aubergine supernatant and be the phycobniliprotein crude extract;
(3) purifying of phycobniliprotein
The crude extract of phycobniliprotein is joined the experiment of saltouing of the saturated sulfate of ammoniac solution of 15%～45% gradient, its saturated sulfate of ammoniac solution gradient arranges following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 ℃ are spent the night, and at 4 ℃ of lower 20000g refrigerated centrifuge 15min, get precipitation, and precipitation is dissolved in the phosphate buffer (pH 6.8) of low concentration, survey its light absorption value, in order to carrying out next step purifying work;
(4) concentrate drying of phycobniliprotein solution
Phycobniliprotein behind the above-mentioned purifying steamed to material with rotation vacuum evaporation instrument be aubergine glue (temperature control is in 40 ℃);
The quality that obtains respectively phycoerythrin after the drying is 0.643g, phycocyanin 0.452g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101947908A CN102871133A (en) | 2011-07-13 | 2011-07-13 | Preparation technology for laver phycobiliprotein powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101947908A CN102871133A (en) | 2011-07-13 | 2011-07-13 | Preparation technology for laver phycobiliprotein powder |
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| CN102871133A true CN102871133A (en) | 2013-01-16 |
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| CN2011101947908A Pending CN102871133A (en) | 2011-07-13 | 2011-07-13 | Preparation technology for laver phycobiliprotein powder |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104687101A (en) * | 2015-02-09 | 2015-06-10 | 福州大学 | Food rich in laver phycobiliprotein |
| CN105146470A (en) * | 2015-08-10 | 2015-12-16 | 中盐榆林盐化有限公司 | Seaweed amino acid salt and preparation method thereof |
| CN106432480A (en) * | 2016-09-07 | 2017-02-22 | 常德炎帝生物科技有限公司 | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing |
-
2011
- 2011-07-13 CN CN2011101947908A patent/CN102871133A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104687101A (en) * | 2015-02-09 | 2015-06-10 | 福州大学 | Food rich in laver phycobiliprotein |
| CN105146470A (en) * | 2015-08-10 | 2015-12-16 | 中盐榆林盐化有限公司 | Seaweed amino acid salt and preparation method thereof |
| CN106432480A (en) * | 2016-09-07 | 2017-02-22 | 常德炎帝生物科技有限公司 | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing |
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Application publication date: 20130116 |