CN102440329A - Preparation technology of porphyra haitanensis phycobiliprotein powder - Google Patents
Preparation technology of porphyra haitanensis phycobiliprotein powder Download PDFInfo
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- CN102440329A CN102440329A CN201010506644XA CN201010506644A CN102440329A CN 102440329 A CN102440329 A CN 102440329A CN 201010506644X A CN201010506644X A CN 201010506644XA CN 201010506644 A CN201010506644 A CN 201010506644A CN 102440329 A CN102440329 A CN 102440329A
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- phycobniliprotein
- porphyra haitanensis
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Abstract
A preparation technology of porphyra haitanensis phycobiliprotein powder belongs to the processing field of food supplement, and particularly relates to the preparation technology of the porphyra haitanensis phycobiliprotein powder. The invention provides the preparation technology of the porphyra haitanensis phycobiliprotein powder, which is simple and effective, and is applicable to mass production. The preparation steps comprise: porphyra haitanensis cell disruption technology; phycobiliprotein crude extract preparation; and phycobiliprotein purification.
Description
Technical field:
The invention belongs to the manufacture field of nutriment, more particularly, relate to the preparation technology of porphyra haitanensis phycobniliprotein powder.
Background technology:
Laver contains special chromoprotein---phycobniliprotein (phycobiliproteins; PBP); This is one type of important photosynthetic pigments albumen composition that is present in red algae, blue-green alge and the latent algae; It can give chlorophyll the luminous energy efficient transfer of catching, thereby makes the photosynthesis of marine alga be able to take place.The content of phycobniliprotein in algae can reach the 25%-28% of dry cell weight; It comprises phycoerythrin (phycoerythrin; PE), phycocyanin (phycocyanin, PC), allophycocyanin (allo-phycocyanin, APC) and phycoerythrocyanin (pec) (phycoery-throcyanin).Phycobniliprotein is a kind of raw material of very important photo-dynamical medicine, but distinctive light sensitive effect auxiliary laser is controlled cancer, in the lesions position enrichment, and efficient generation free radical and active attitude oxygen after the absorbing light, thus realize killing and wounding to tumour cell.Phototoxicity is arranged and do not have dark toxicity, fast the human body metabolism, phycobniliprotein also has oxidation and removing free radicals and the effect that improves immunity.The immunofluorescence label antibody of its making; Detection sensitivity is higher than traditional fluorescent marker far away; Can be used for particular molecule location and detect, comprise the immune molecule detection of SARS virus with important molecule, exactly diagnosing tumour, confirm diseased region and severe extent etc.Phycobniliprotein can be used as function factor and is used for health food and functional food and special feed, still is desirable natural colouring matter simultaneously, can be used for food industry and cosmetics industry etc. in a large number, have no side effect, and be safe additive.In addition, current research finds that aspects such as it is stored and handle in optical information, photodetection fast, artificial neural network also have potential application prospect.But because the difficulty of phycobniliprotein separation and purification, existing phycobniliprotein commodity price is expensive, makes its application receive certain restriction.Although there are many people to attempt phycobiliprotein gene is changed in the microorganism to realize the large-scale production of phycobniliprotein; Also have very long stretch to walk but produce phycobniliprotein, separate phycobniliprotein from extracting algae at present and be still the main path that obtains phycobniliprotein with transgenic method.
China is also very little to the dynamics and the degree of depth of algae development and use, and major part only limits to simple edible, and exported product also is only limited to early-products, does not also use high-tech and carries out profound processing and utilization.Some high-tech deep processed products of algae then mainly rely on import, and are very expensive as the drug use price.The production of phycoerythrin at present is basically by U.S. Sigma company monopolizing, and domestic have with Bangiales, purple ball algae and the spirulina report as raw material extraction phycoerythrin, still is in conceptual phase at present, do not have finished product to come into the market to sell.China marine site is broad, and algae resource is very abundant, is maximum in the world laver big producing country, and it is coastal that China's laver culture and processing mainly concentrate on Soviet Union, Zhejiang, Fujian again, and its output accounts for more than 95% of the whole nation.The market price that Rudong County, Jiangsu Province Porphyra yezoensis output accounts for 50%. lavers of national total amount is 1/10 of a Bangiales, 1/3 of chlorella and spirulina.Select for use laver to extract phycobniliprotein, it is low to have a cost, the advantage that yield is high.Its major part of phycobniliprotein in the laver mainly is phycoerythrin (PE).Because the phycobniliprotein isolation and purification method reported is complicated, cause that production cost is too high can't to be prepared to satisfy the market potential demand in a large number.
Summary of the invention:
The present invention is exactly to the problems referred to above, and a kind of preparation technology of simple and effective, suitable mass-produced porphyra haitanensis phycobniliprotein powder is provided.
For realizing above-mentioned purpose of the present invention, processing step of the present invention is:
1, porphyra haitanensis clasmatosis technology
With pulverizer laver is pulverized earlier, the laver powder after will pulverizing is then put into water and was soaked 5～10 hours, takes out soak, pulverizes with ultrasonic wave.
2, the preparation of phycobniliprotein crude extract
Directly, make its swelling, break, discharge phycobniliprotein with distilled water or less salt solution soaking frond cell.Distilled water immersion takes 10 days, and the less salt solution soaking takes 3～4 days.
Take by weighing the 5.99g laver powder, use distilled water immersion, fully swelling.After 12 days, filter with 74 microns filter clothes, and will filtrate at 4 ℃ of centrifugal 20min of following 5500g, remove the frond residue, get the aubergine supernatant and be the phycobniliprotein crude extract.
3, the purifying of phycobniliprotein
The crude extract of phycobniliprotein is joined the experiment of saltouing of the saturated sulfate of ammoniac solution of 15%～45% gradient; Its saturated sulfate of ammoniac solution gradient is provided with following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 ℃ and spends the night, and at 4 ℃ of following 20000g refrigerated centrifuge 15min; Get deposition; And deposition is dissolved in the phosphate buffer (pH 6.8) of low concentration, survey its light absorption value, in order to carrying out next step purifying work.
4, the concentrate drying of phycobniliprotein solution
Phycobniliprotein behind the above-mentioned purifying steamed to material with rotation vacuum evaporation appearance be aubergine glue (temperature control is in 40 ℃).
The quality that obtains phycoerythrin after the drying respectively is 0.643g, phycocyanin 0.452g.
Description of drawings:
Fig. 1 is preparation technology's flow chart of the present invention.
The specific embodiment:
The present invention is raw material with the porphyra haitanensis, and porphyra haitanensis phycobniliprotein powder is prepared, and the preparation process of this invention is:
1, porphyra haitanensis clasmatosis technology
With pulverizer laver is pulverized earlier, the laver powder after will pulverizing is then put into water and was soaked 5～10 hours, takes out soak, pulverizes with ultrasonic wave.
2, the preparation of phycobniliprotein crude extract
Directly, make its swelling, break, discharge phycobniliprotein with distilled water or less salt solution soaking frond cell.Distilled water immersion takes 10 days, and the less salt solution soaking takes 3～4 days.
Take by weighing the 5.99g laver powder, use distilled water immersion, fully swelling.After 12 days, filter with 74 microns filter clothes, and will filtrate at 4 ℃ of centrifugal 20min of following 5500g, remove the frond residue, get the aubergine supernatant and be the phycobniliprotein crude extract.
3, the purifying of phycobniliprotein
The crude extract of phycobniliprotein is joined the experiment of saltouing of the saturated sulfate of ammoniac solution of 15%～45% gradient; Its saturated sulfate of ammoniac solution gradient is provided with following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 ℃ and spends the night, and at 4 ℃ of following 20000g refrigerated centrifuge 15min; Get deposition; And deposition is dissolved in the phosphate buffer (pH 6.8) of low concentration, survey its light absorption value, in order to carrying out next step purifying work.
4, the concentrate drying of phycobniliprotein solution
Phycobniliprotein behind the above-mentioned purifying steamed to material with rotation vacuum evaporation appearance be aubergine glue (temperature control is in 40 ℃).
The quality that obtains phycoerythrin after the drying respectively is 0.643g, phycocyanin 0.452g.
Claims (1)
1. porphyra haitanensis phycobniliprotein powder is characterized in that the present invention is raw material with the porphyra haitanensis, and porphyra haitanensis phycobniliprotein powder is prepared, and preparation process of the present invention is:
(1) porphyra haitanensis clasmatosis technology
With pulverizer laver is pulverized earlier, the laver powder after will pulverizing is then put into water and was soaked 5～10 hours, takes out soak, pulverizes with ultrasonic wave;
(2) preparation of phycobniliprotein crude extract
Directly, make its swelling, break, discharge phycobniliprotein with distilled water or less salt solution soaking frond cell; Distilled water immersion takes 10 days, and the less salt solution soaking takes 3～4 days;
Take by weighing the 5.99g laver powder, use distilled water immersion, fully swelling; After 12 days, filter with 74 microns filter clothes, and will filtrate at 4 ℃ of centrifugal 20min of following 5500g, remove the frond residue, get the aubergine supernatant and be the phycobniliprotein crude extract;
(3) purifying of phycobniliprotein
The crude extract of phycobniliprotein is joined the experiment of saltouing of the saturated sulfate of ammoniac solution of 15%～45% gradient; Its saturated sulfate of ammoniac solution gradient is provided with following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 ℃ and spends the night, and at 4 ℃ of following 20000g refrigerated centrifuge 15min; Get deposition; And deposition is dissolved in the phosphate buffer (pH 6.8) of low concentration, survey its light absorption value, in order to carrying out next step purifying work;
(4) concentrate drying of phycobniliprotein solution
Phycobniliprotein behind the above-mentioned purifying steamed to material with rotation vacuum evaporation appearance be aubergine glue (temperature control is in 40 ℃);
The quality that obtains phycoerythrin after the drying respectively is 0.643g, phycocyanin 0.452g.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010506644XA CN102440329A (en) | 2010-10-14 | 2010-10-14 | Preparation technology of porphyra haitanensis phycobiliprotein powder |
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| CN201010506644XA CN102440329A (en) | 2010-10-14 | 2010-10-14 | Preparation technology of porphyra haitanensis phycobiliprotein powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106432480A (en) * | 2016-09-07 | 2017-02-22 | 常德炎帝生物科技有限公司 | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing |
| CN109734772A (en) * | 2019-03-11 | 2019-05-10 | 中国水产科学研究院南海水产研究所 | A kind of extracting method of end water porphyra haitanensis protein |
| CN110483627A (en) * | 2019-08-09 | 2019-11-22 | 自然资源部第三海洋研究所 | The extracting method and its application of phycoerythrin extracting solution |
| CN112812176A (en) * | 2021-01-13 | 2021-05-18 | 江南大学 | Method for extracting phycocyanin from spirulina by low-salt flocculation method |
| CN114760849A (en) * | 2019-07-30 | 2022-07-15 | 特洛弗克有限责任公司 | Plant-based food |
-
2010
- 2010-10-14 CN CN201010506644XA patent/CN102440329A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106432480A (en) * | 2016-09-07 | 2017-02-22 | 常德炎帝生物科技有限公司 | Extraction method of phycobiliprotein of nostoc sphaeroides kutzing |
| CN109734772A (en) * | 2019-03-11 | 2019-05-10 | 中国水产科学研究院南海水产研究所 | A kind of extracting method of end water porphyra haitanensis protein |
| CN114760849A (en) * | 2019-07-30 | 2022-07-15 | 特洛弗克有限责任公司 | Plant-based food |
| CN110483627A (en) * | 2019-08-09 | 2019-11-22 | 自然资源部第三海洋研究所 | The extracting method and its application of phycoerythrin extracting solution |
| CN112812176A (en) * | 2021-01-13 | 2021-05-18 | 江南大学 | Method for extracting phycocyanin from spirulina by low-salt flocculation method |
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Application publication date: 20120509 |