CN104479010A - Preparation process of wall broken spirulina protein powder - Google Patents
Preparation process of wall broken spirulina protein powder Download PDFInfo
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- CN104479010A CN104479010A CN201410793528.9A CN201410793528A CN104479010A CN 104479010 A CN104479010 A CN 104479010A CN 201410793528 A CN201410793528 A CN 201410793528A CN 104479010 A CN104479010 A CN 104479010A
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
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Abstract
The invention discloses a preparation process of wall broken spirulina protein powder. The preparation process of the wall broken spirulina protein powder comprises the following steps of: pulverizing wall broken spirulina with a pulverizer, soaking wall broken spirulina powder in water for 5-10 hours, taking out the soaked wall broken spirulina powder, and pulverizing under ultrasonic waves; soaking spirulina cells with distilled water or a low-salt solution directly so that the spirulina cells swell and break to release wall broken spirulina protein, wherein the wall broken spirulina protein is soaked in distilled water for 10 days, or the wall broken spirulina protein is soaked in the low-salt solution for 3-4 days; weighing 5.99g of wall broken spirulina protein powder, and soaking in distilled water for 12 days for swelling fully; filtering with a piece of filtering cloth of 74mu m, centrifuging a filtrate at the temperature of 4 DEG C and 5500g for 20 minutes, removing spirulina residues, and taking a purplish red supernatant which is the crude extract of wall broken spirulina protein. The preparation process of the wall broken spirulina protein powder is simple, and the prepared wall broken spirulina protein powder has a high nutritional value.
Description
Technical field
The present invention relates to a kind of preparation technology of broken wall spirulina protein powder.
Background technology
Due to the distinctive medicinal and nutritional values of spirulina, the parent of people is enjoyed to look at.At present, the food of people is varied, meets needs to a certain extent, but along with the development of society, standard of living improves, the dietetic requirement of people also there occurs change, and the wheaten food that the existing trophic function of people's an urgent demand has again diet function is satisfied the demand.
Spirulina is also that a kind of photosynthetic autotrophs are biological, containing photosynthetic synusia (thylakoid), VITAMIN, crude protein, crude fat, carbohydrate, carotene etc. in its frustule.Spirulina contains rich in protein, up to 60-70%.Spirulina, containing abundant beta carotene, is 15 times of Radix Dauci Sativae, is 40-60 times of spinach.Spirulina contains abundant VITMAIN B1, B2, B5, B6, B11, B12, C, E.Vitamin B group is of a great variety, and the physiological function that each tool is special, and any one lacked wherein all may cause disease.Spirulina contains the trace element of multiple needed by human, calcium, magnesium, sodium, potassium, phosphorus, iodine, selenium, iron, copper, zinc etc., and iron deficiency can cause anaemia, and scarce zinc can cause dysplasia; Selenium can activate DNA repair enzyme, and the generation of immune stimulatory sphaeroprotein and antibody, catches free radical, and the toxicity of reduction or opposed body some metal interior, suppresses the carcinogenesis of some carcinogenic substances.Selenium can also prevent puna.Chlorophyll A contained in spirulina, has the clean blood function of the hematopoiesis of its uniqueness.
Phycobiliprotein (comprising Phycocyanins, C-, allophycocyanin etc.), is present in spirulina, is active substance important in spirulina, has the special property transmitted light energy absorption, has been used as the photosensitizers of cancer laser therapy.Research display, phycobiliprotein also has a series of important physiologically active, and it receives much concern to the restraining effect of tumour cell, and in order to improve anti-tumor activity, the research of rich selenium phycobiliprotein also becomes increasingly active.Recent study proves, Se-enriched phycocyanin is obviously better than selenium or Phycocyanins, C-to the antagonistic action of Experimental Hepatic Injuries; Selenium enriched Spirulina extracting solution can significantly improve experiment mice immunizing power; Utilize the Se-enriched phycocyanin of purifying from selenium enriched Spirulina, preparation photofixation biomaterial, has remarkable restraining effect to vitro culture liver cancer, Growth of Gastric.In selenium enriched Spirulina, the content of seleno-protein is hundred times of common algae, and be the organoselenium supplement of human body highly effective and safe, human body can be digested and assimilated and reach more than 95%.In theory, selenium enriched Spirulina has selenium and spirulina-active material double effects concurrently, and existing result of study progressively proves this inference.So selenium enriched Spirulina is the food proteins source of ideals of human being and organic selenium source, press down cancer and the radioprotective research of selenium enriched Spirulina also obtain encouraging progress.
At present, domestic and international selenium enriched Spirulina product still mainly rests on the level of full algae powder, needs large dose oral administration just effective as protective foods, but selenium enriched Spirulina is as vegetable cell, cell walls is thicker, is not easy digested absorption, often take more than 10-30 gram day by day, even broken through cell walls, also waste is a lot, adds the own meta-alkali of frond (pH8.0-10), take in meeting in a large number and hydrochloric acid in gastric juice, affect the digestive function of stomach, selenium enriched Spirulina is with unpleasant fishy smell in addition, taste extreme difference.
Summary of the invention
The object of the present invention is to provide a kind of preparation technology of broken wall spirulina protein powder.
For achieving the above object, the present invention implements by the following technical solutions:
The preparation technology of broken wall spirulina protein powder, comprises the steps:
(1) spirulina pulverizer is carried out coarse reduction and obtain spirulina meal;
(2) spirulina meal is put into the feeding trough of twin-screw extruder with the input speed of 25 ~ 30kg/h, in extrusion machine auxiliary material ingress, water is carried out humidification with the form of spraying to spirulina meal simultaneously; The rotating speed that described twin-screw extruder squeezes setting is 100 ~ 140r/min, and temperature is 130 ~ 150 DEG C, and adding moisture is 2 ~ 3kg/h;
(3) by step 2) spirulina powder that obtains carries out micronizing by pulverizer and obtains spirulina super-fine powder; The inlet amount of described duplex algae powder is 20 ~ 30kg; The condition of described micronizing is: pulverizer rotating speed is 5000 ~ 140r/min, and grinding time is 5 ~ 10min;
(4) then the broken wall spirulina powder after micronizing is put into water to soak 5 ~ 10 hours, take out soak, pulverize by ultrasonic wave; The ultrasonic disruption working hour is 6 ~ 8s, intermittent time is 6 ~ 8s, the broken time is 10 ~ 100 minutes, in order to prevent, temperature is too high makes protein denaturation, time broken, the temperature of frond should control within 12 DEG C, filter-cloth filtering, and filtrate is at 7000 ~ 15000 rpm high speed centrifugation 30 ~ 50min, go precipitation, ultra-filtration membrane crossed by supernatant liquor;
(5) direct distilled water or low salt solutions soak frond cell, make that it is swelling, break, discharge broken wall spirulina protein; Distilled water immersion takes 10 days, and low salt solutions soaks and takes 3 ~ 4 days; Take 5.99g broken wall spirulina powder, with distilled water immersion, fully swelling; After 12 days, with the filter-cloth filtering of 74 microns, and by filtrate centrifugal 20min of 5500g at 4 DEG C, remove frond residue, get red-purple supernatant liquor and be broken wall spirulina protein crude extract;
(6) crude extract of broken wall spirulina protein is joined the saturated sulfate solution of 15% ~ 45% gradient and carry out experiment of saltouing, its saturated sulfate solution gradient arranges following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 DEG C are spent the night, and at 4 DEG C 20000g frozen centrifugation 15min, get precipitation, and precipitation is dissolved in the phosphate buffered saline buffer (pH 6.8) of lower concentration, survey its light absorption value, in order to carrying out next step purification work;
(7) by after above-mentioned purifying broken wall spirulina protein rotary vacuum evaporator steam to material be red-purple glue (temperature control is within 40 DEG C); The quality obtaining phycoerythrin after drying is respectively 0.643g, Phycocyanins, C-0.452g;
(8) add appropriate soybean protein isolate, obtain finished product.
The present invention prepares that extraction process is simple, cost is low, and be suitable for the Spirulina phycocyanin finished product that high purity, high yield are processed in intermittent and large-scale production, the broken wall spirulina protein powder of gained is of high nutritive value.
Crushing efficiency of the present invention is high, and the time is short, and in algae, the extraction yield of active substance is high, material function vital preservation is intact, and automated operation, can be used for industrialization scale operation; Have that rate of drying is fast, the time is short, temperature of charge is low, the advantage such as color, smell and taste and nutritive ingredient are retained;
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The preparation technology of a kind of broken wall spirulina protein powder of the present invention, comprises the steps:
(1) spirulina pulverizer is carried out coarse reduction and obtain spirulina meal;
(2) spirulina meal is put into the feeding trough of twin-screw extruder with the input speed of 25 ~ 30kg/h, in extrusion machine auxiliary material ingress, water is carried out humidification with the form of spraying to spirulina meal simultaneously; The rotating speed that described twin-screw extruder squeezes setting is 100 ~ 140r/min, and temperature is 130 ~ 150 DEG C, and adding moisture is 2 ~ 3kg/h;
(3) by step 2) spirulina powder that obtains carries out micronizing by pulverizer and obtains spirulina super-fine powder; The inlet amount of described duplex algae powder is 20 ~ 30kg; The condition of described micronizing is: pulverizer rotating speed is 5000 ~ 140r/min, and grinding time is 5 ~ 10min;
(4) then the broken wall spirulina powder after micronizing is put into water to soak 5 ~ 10 hours, take out soak, pulverize by ultrasonic wave; The ultrasonic disruption working hour is 6 ~ 8s, intermittent time is 6 ~ 8s, the broken time is 10 ~ 100 minutes, in order to prevent, temperature is too high makes protein denaturation, time broken, the temperature of frond should control within 12 DEG C, filter-cloth filtering, and filtrate is at 7000 ~ 15000 rpm high speed centrifugation 30 ~ 50min, go precipitation, ultra-filtration membrane crossed by supernatant liquor;
(5) direct distilled water or low salt solutions soak frond cell, make that it is swelling, break, discharge broken wall spirulina protein; Distilled water immersion takes 10 days, and low salt solutions soaks and takes 3 ~ 4 days; Take 5.99g broken wall spirulina powder, with distilled water immersion, fully swelling; After 12 days, with the filter-cloth filtering of 74 microns, and by filtrate centrifugal 20min of 5500g at 4 DEG C, remove frond residue, get red-purple supernatant liquor and be broken wall spirulina protein crude extract;
(6) crude extract of broken wall spirulina protein is joined the saturated sulfate solution of 15% ~ 45% gradient and carry out experiment of saltouing, its saturated sulfate solution gradient arranges following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 DEG C are spent the night, and at 4 DEG C 20000g frozen centrifugation 15min, get precipitation, and precipitation is dissolved in the phosphate buffered saline buffer (pH 6.8) of lower concentration, survey its light absorption value, in order to carrying out next step purification work;
(7) by after above-mentioned purifying broken wall spirulina protein rotary vacuum evaporator steam to material be red-purple glue (temperature control is within 40 DEG C); The quality obtaining phycoerythrin after drying is respectively 0.643g, Phycocyanins, C-0.452g;
(8) add appropriate soybean protein isolate, obtain finished product.
The present invention prepares that extraction process is simple, cost is low, and be suitable for the Spirulina phycocyanin finished product that high purity, high yield are processed in intermittent and large-scale production, the broken wall spirulina protein powder of gained is of high nutritive value.
Crushing efficiency of the present invention is high, and the time is short, and in algae, the extraction yield of active substance is high, material function vital preservation is intact, and automated operation, can be used for industrialization scale operation; Have that rate of drying is fast, the time is short, temperature of charge is low, the advantage such as color, smell and taste and nutritive ingredient are retained
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (1)
1. the preparation technology of broken wall spirulina protein powder, is characterized in that, comprises the steps:
(1) spirulina pulverizer is carried out coarse reduction and obtain spirulina meal;
(2) spirulina meal is put into the feeding trough of twin-screw extruder with the input speed of 25 ~ 30kg/h, in extrusion machine auxiliary material ingress, water is carried out humidification with the form of spraying to spirulina meal simultaneously; The rotating speed that described twin-screw extruder squeezes setting is 100 ~ 140r/min, and temperature is 130 ~ 150 DEG C, and adding moisture is 2 ~ 3kg/h;
(3) by step 2) spirulina powder that obtains carries out micronizing by pulverizer and obtains spirulina super-fine powder; The inlet amount of described duplex algae powder is 20 ~ 30kg; The condition of described micronizing is: pulverizer rotating speed is 5000 ~ 140r/min, and grinding time is 5 ~ 10min;
(4) then the broken wall spirulina powder after micronizing is put into water to soak 5 ~ 10 hours, take out soak, pulverize by ultrasonic wave; The ultrasonic disruption working hour is 6 ~ 8s, intermittent time is 6 ~ 8s, the broken time is 10 ~ 100 minutes, in order to prevent, temperature is too high makes protein denaturation, time broken, the temperature of frond should control within 12 DEG C, filter-cloth filtering, and filtrate is at 7000 ~ 15000 rpm high speed centrifugation 30 ~ 50min, go precipitation, ultra-filtration membrane crossed by supernatant liquor;
(5) direct distilled water or low salt solutions soak frond cell, make that it is swelling, break, discharge broken wall spirulina protein; Distilled water immersion takes 10 days, and low salt solutions soaks and takes 3 ~ 4 days; Take 5.99g broken wall spirulina powder, with distilled water immersion, fully swelling; After 12 days, with the filter-cloth filtering of 74 microns, and by filtrate centrifugal 20min of 5500g at 4 DEG C, remove frond residue, get red-purple supernatant liquor and be broken wall spirulina protein crude extract;
(6) crude extract of broken wall spirulina protein is joined the saturated sulfate solution of 15% ~ 45% gradient and carry out experiment of saltouing, its saturated sulfate solution gradient arranges following 15%, 20%, 25%, 30%, 35%, 40%, 45%, 4 DEG C are spent the night, and at 4 DEG C 20000g frozen centrifugation 15min, get precipitation, and precipitation is dissolved in the phosphate buffered saline buffer (pH 6.8) of lower concentration, survey its light absorption value, in order to carrying out next step purification work;
(7) by after above-mentioned purifying broken wall spirulina protein rotary vacuum evaporator steam to material be red-purple glue (temperature control is within 40 DEG C); The quality obtaining phycoerythrin after drying is respectively 0.643g, Phycocyanins, C-0.452g;
(8) add appropriate soybean protein isolate, obtain finished product.
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| CN201410793528.9A CN104479010A (en) | 2014-12-20 | 2014-12-20 | Preparation process of wall broken spirulina protein powder |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105110909A (en) * | 2015-08-05 | 2015-12-02 | 国家海洋局第一海洋研究所 | Preparation method for marine biological calcium magnesium fertilizer |
| CN105146470A (en) * | 2015-08-10 | 2015-12-16 | 中盐榆林盐化有限公司 | Seaweed amino acid salt and preparation method thereof |
| CN108794565A (en) * | 2018-04-27 | 2018-11-13 | 胡梦丽 | A kind of preparation method of spirulina protein powder |
| CN111728210A (en) * | 2020-06-17 | 2020-10-02 | 福建省神六保健食品有限公司 | Spirulina camel colostrum protein powder and processing technology thereof |
| CN111748478A (en) * | 2020-06-28 | 2020-10-09 | 深圳大学 | Microalgae wall-breaking processing method and application thereof |
| CN114341165A (en) * | 2019-07-03 | 2022-04-12 | 鲁门生物科学股份有限公司 | Non-parenteral therapy delivery platform for arthrospira platensis |
| CN114478813A (en) * | 2022-01-24 | 2022-05-13 | 徐州工程学院 | Method for extracting polysaccharide from hericium erinaceus spore powder |
| CN115109146A (en) * | 2022-08-04 | 2022-09-27 | 北京岳达生物科技有限公司 | Algae protein extracted from algae and preparation method thereof |
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| CN101942014A (en) * | 2010-08-23 | 2011-01-12 | 中国科学院烟台海岸带研究所 | Food grade phycocyanin and preparation method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105110909A (en) * | 2015-08-05 | 2015-12-02 | 国家海洋局第一海洋研究所 | Preparation method for marine biological calcium magnesium fertilizer |
| CN105146470A (en) * | 2015-08-10 | 2015-12-16 | 中盐榆林盐化有限公司 | Seaweed amino acid salt and preparation method thereof |
| CN108794565A (en) * | 2018-04-27 | 2018-11-13 | 胡梦丽 | A kind of preparation method of spirulina protein powder |
| CN114341165A (en) * | 2019-07-03 | 2022-04-12 | 鲁门生物科学股份有限公司 | Non-parenteral therapy delivery platform for arthrospira platensis |
| CN111728210A (en) * | 2020-06-17 | 2020-10-02 | 福建省神六保健食品有限公司 | Spirulina camel colostrum protein powder and processing technology thereof |
| CN111748478A (en) * | 2020-06-28 | 2020-10-09 | 深圳大学 | Microalgae wall-breaking processing method and application thereof |
| CN111748478B (en) * | 2020-06-28 | 2023-02-03 | 深圳大学 | Microalgae wall-breaking processing method and application thereof |
| CN114478813A (en) * | 2022-01-24 | 2022-05-13 | 徐州工程学院 | Method for extracting polysaccharide from hericium erinaceus spore powder |
| CN114478813B (en) * | 2022-01-24 | 2023-03-10 | 徐州工程学院 | Method for extracting polysaccharide from hericium erinaceus spore powder |
| CN115109146A (en) * | 2022-08-04 | 2022-09-27 | 北京岳达生物科技有限公司 | Algae protein extracted from algae and preparation method thereof |
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Application publication date: 20150401 |