CN108794565A - A kind of preparation method of spirulina protein powder - Google Patents
A kind of preparation method of spirulina protein powder Download PDFInfo
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- CN108794565A CN108794565A CN201810396433.1A CN201810396433A CN108794565A CN 108794565 A CN108794565 A CN 108794565A CN 201810396433 A CN201810396433 A CN 201810396433A CN 108794565 A CN108794565 A CN 108794565A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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Abstract
The invention belongs to field of biochemical separation and purifying technology, are related to a kind of preparation method of spirulina protein powder, include the following steps：Water after spirulina is crushed first with 8~10 times stirs evenly, then spirulina powder fluid is placed in Homogenization Treatments in high pressure homogenizer, release the spirulina protein after broken wall, spirulina protein pressure after broken wall is pricked into out protein liquid, protein liquid is thawed to extract and is placed on dissolved air flotation purifying plant DNA purity, the protein liquid finally dried after purification obtains spirulina protein powder, the spirulina protein powder, preparation method thereof of the present invention solves spirulina protein powder method of purification in the prior art there are process routes long, extracting cycle is long, the problems such as high energy consumption and low production efficiency.
Description
Technical field
The invention belongs to field of biochemical separation and purifying technology, are related to a kind of preparation method of spirulina protein powder, especially
A kind of spirulina protein powder, preparation method thereof purified by dissolved air flotation purifying plant.
Background technology
Spirulina is a kind of photosynthetic autotrophs biology, contains photosynthetic synusia (thylakoid), vitamin, thick egg in frustule
In vain, crude fat, carbohydrate, carrotene etc..Spirulina is containing abundant protein, up to 60-70%.Spirulina is containing abundant
Bata-carotene, be 15 times of carrot, be 40-60 times of spinach.Spirulina containing abundant vitamin B1, B2, B5, B6, B11,
B12,C,E.B family vitamin type is various, and respectively has special physiological function, and lacking any type therein may all cause
Disease.Spirulina contains a variety of the essential trace elements of the human bodys, calcium, magnesium, sodium, potassium, phosphorus, iodine, selenium, iron, copper, zinc etc., and iron deficiency can be led
Cause anaemia, zinc-deficiency that can lead to depauperation；Selenium can activate DNA repair enzymes, stimulate the generation of immune globulin bletilla antibody, capture is certainly
By base, reduce or opposed body in certain metals toxicity, inhibit the carcinogenesis of some carcinogens.Selenium can also prevent high mountain
Disease.Chlorophyll A contained in spirulina has its unique net blood function of hematopoiesis.
Phycobniliprotein (including phycocyanin, allophycocyanin etc.) is present in spirulina, for work important in spirulina
Property substance, there is the special nature transmitted to light energy absorption, be used as the photosensitizer of cancer laser therapy.Research is aobvious
Show, phycobniliprotein also has a series of important physiological activity, is concerned to the inhibiting effect of tumour cell, in order to improve
The research of antitumor activity, selenium-rich phycobniliprotein also becomes increasingly active.Recent study proves that Se-enriched phycocyanin is to mouse experiment
The antagonism of property hepatic injury is significantly stronger than selenium or phycocyanin；It is immune that selenium enriched Spirulina extracting solution is remarkably improved experiment mice
Power；Using the Se-enriched phycocyanin purified from selenium enriched Spirulina, photofixation biomaterial is prepared, in vitro culture liver cancer, stomach
Growth of cancer cells, which has, significantly inhibits effect.The content of selenoprotein is hundred times of common algae in selenium enriched Spirulina, is that human body is efficient
Organic selenium supplement of safety, human body can be digested and assimilated up to 95% or more.Theoretically, selenium enriched Spirulina has both selenium and spirulina is lived
Property substance double effects, existing result of study gradually prove this inference.So selenium enriched Spirulina is the ideal food of the mankind
Protein sources and organic selenium source, the suppression cancer of selenium enriched Spirulina and radioresistance research also obtain encouraging progress.
Currently, selenium enriched Spirulina product still predominantly stays in the level of full algae powder both at home and abroad, reported about spirulina
The extracting method of albumen powder includes mainly that the techniques such as nature swelling method, multigelation method, supercritical ultrasonics technology and enzymatic isolation method are former from algae
Spirulina protein is extracted in material, but it exists in the actual production process that process route is long, extracting cycle is long, high energy consumption, efficiency
Low disadvantage causes the spirulina protein market price of high-purity expensive, constrains spirulina protein and circulate in the market.
Invention content
In view of this, the present invention is in order to solve spirulina protein powder method of purification in the prior art, there are process routes
The problems such as long, extracting cycle length, high energy consumption and low production efficiency, provide a kind of preparation method of spirulina protein powder.
In order to achieve the above objectives, the present invention provides a kind of preparation method of spirulina protein powder, includes the following steps：
A, spirulina is crushed by pulverizer, obtains the spirulina powder that mesh number is 1200~1500 mesh, wherein powder
Broken machine rotating speed is 5000~1000r/min, and grinding time is 10~15min；
B, the water that 8~10 times are added in the spirulina powder after crushing being stirred evenly, whipping temp is controlled at 15~20 DEG C,
Mixing time is 8~10h, and the spirulina powder fluid after uniform stirring, which is placed in high pressure homogenizer, carries out homogenize process, makes spiral shell
Spirulina powder is swollen, ruptures, releasing the spirulina protein after broken wall；
C, 3% calcium chloride will be added in the spirulina protein after broken wall, broken wall spirulina is squeezed out by briquetting apparatus
Protein liquid in albumen, briquetting apparatus temperature are controlled at 0 DEG C or less；
D, 0~5 DEG C of defrosting of protein liquid that will be pricked out, thawing time is 5~7h, and albumen is extracted in 5 DEG C of environment below
Liquid；
E, the protein liquid of extraction is extracted by dissolved air flotation purifying plant again after the filtering of Ultra filtration membrane system pure
Degree, purity reach 60% or more, and wherein dissolved air flotation purifying plant includes columned biochemical container, barostat, speed
The feed pipe with inlet valve is installed in adjuster and recycling bin, the center at the top of biochemical container, is arranged and returns outside biochemical container
The impurity collection that receipts bucket communicates is enclosed, and four groups are uniformly distributed on impurity collection circle and is in bending and is communicated with biochemical container top miscellaneous
Matter separating pipe, opens up on the inner wall of biochemical container and the matched diversion trench of impurity separating pipe, installation connection speed in biochemical container
Spend the agitating device of adjuster, barostat connect with biochemical container pipeline, wherein the pressure limit of barostat for 3~
The velocity interval of 5 kilograms of pressure, speed regulator is 2300~2500r/min；
F, protein liquid after purification is obtained into spirulina protein powder by high speed centrifugation drying machine drying.
Further, the temperature of step B mesohighs homogenizer processing spirulina powder is controlled at 12 DEG C hereinafter, preventing temperature excessively high
Make the albuminous degeneration in spirulina powder.
Further, the briquetting apparatus in step C is pneumatic cylinder, and pneumatic cylinder is automatically to the protein liquid in spirulina protein
Substep squeezes.
Further, agitating device is the revolving vane in biochemical container in step E, and the number of revolving vane is strange
The deflection angle of number, revolving vane is 5 °.
Further, first rotating shaft is installed in the middle part of revolving vane in step E, second turn is equipped on speed regulator
Axis is equipped with driven wheel below first rotating shaft, driving wheel is equipped with below the second shaft, driving wheel and driven wheel pass through skin
Band connection.
Further, first rotating shaft is in hollow form in step E, and barostat pipeline is connected in first rotating shaft inner cavity.
Further, the temperature in step F drying processes controls within 40 DEG C.
The beneficial effects of the present invention are：
1, the preparation method of spirulina protein powder of the present invention uniformly mixes the spirulina after crushing with 8~10 times of water
Afterwards, the homogenize process in high pressure homogenizer makes spirulina powder be swollen, rupture, releases the spirulina protein after broken wall.Wherein
High pressure homogenizer is mainly made of high-pressure homogeneous chamber and booster body, and the inside of high-pressure homogeneous chamber has specially designed geometric form
Shape, under the action of booster body, quickly by homogeneous chamber, material can be shaken by high speed shear, high frequency high-pressure solution simultaneously
Swing, cavitation and convection current hit etc. mechanical forces and corresponding fuel factor, the mechanical force and chemical effect thus caused can
Physics, chemistry and the structural property of induction material macromolecular change, and are finally reached the effect of homogeneous so that spirulina powder
Cell wall rupture releases spirulina protein.3% calcium chloride is added in spirulina protein after broken wall, calcium chloride is by spiral
Algae albumen is washed, and prevents spirulina protein from remaining on spirulina shell.The spirulina protein liquid extracted is in air supporting point
From being purified in purifying plant so that protein liquid DNA purity reaches 60% or more, the extraction spirulina protein it is simple for process, at
This is low, is suitable for the spirulina protein powder of intermittent and large-scale production processing high-purity, high concentration, the spirulina protein of gained
Powder nutritive value is high.
2, the dissolved air flotation purifying plant in spirulina protein powder, preparation method thereof of the present invention, the feed pipe at the top of biochemical container
For throwing spirulina protein powder, biochemical container is used for placing spirulina protein powder, and the agitating device in biochemical container is used for stirring
Spirulina protein powder is mixed, the barostat of biochemical container side is used for controlling the air pressure in biochemical container so that spirulina egg
Impurity in white powder is separated after reaching certain critical point from spirulina protein powder, while making spirulina protein and water
Detached, under the high speed rotation of agitating device, by the light impurities in spirulina protein powder by physics mode throw away to
In impurity separating pipe, and then circle is collected by impurity and is collected in special recycling bin, reduces pollution of the waste to environment.Together
When the dissolved air flotation purifying plant, the extraction of spirulina protein powder and the separation of light impurities are combined into one, technique is simplified
Flow improves the service efficiency and disintegrate-quality of product.In addition, in production process by the way of physical apparatus, reducing
The use for learning substance, maintains the original physical attribute of raw material so that finished product is more pure natural to greatest extent.
3, the preparation method of spirulina protein powder of the present invention, crushing efficiency is high, and the broken time is short, active material in spirulina
Recovery rate is high, material function vital preservation is intact, and automation mechanized operation is suitable for industrialization large-scale production, has drying
The advantages that speed is fast, the time is short, temperature of charge is low, color, smell and taste and nutritional ingredient are retained.
4, the preparation method of spirulina protein powder of the present invention is a kind of physical method based on mechanical equipment, by this
Spirulina protein powder prepared by preparation method, ensure that natural sex, the safety of product, improves spirulina protein in product
Content reduces production cost and period, reduces the waste of resource.
Description of the drawings
In order to keep the purpose of the present invention, technical solution and advantageous effect clearer, the present invention provides following attached drawing and carries out
Explanation：
Fig. 1 is the structural schematic diagram of the dissolved air flotation purifying plant of spirulina protein powder of the present invention.
Specific implementation mode
The preferred embodiment of the present invention will be described in detail below.
Reference numeral in Figure of description includes：
Biochemical container 1, feed pipe 2, impurity separating pipe 3, recycling bin 4, impurity collect circle 5, barostat 6, speed tune
Save device 7, belt 8, revolving vane 9, inlet valve 10.
Embodiment 1
A kind of preparation method of spirulina protein powder, includes the following steps：
A, the spirulina of 125kg is crushed by pulverizer, obtains the spirulina powder that mesh number is 1200~1500 mesh,
Wherein pulverizer rotating speed is 5000~1000r/min, and grinding time is 10~15min；
B, the water that 1000kg is added in the spirulina powder after crushing is stirred evenly, whipping temp is controlled at 20 DEG C, stirring
Time is 8h, and the spirulina powder fluid after uniform stirring, which is placed in high pressure homogenizer, carries out homogenize process, keeps spirulina powder molten
It is swollen, rupture, release the spirulina protein after broken wall, wherein the temperature control of high pressure homogenizer processing spirulina powder 12 DEG C with
Under, prevent the excessively high albuminous degeneration made in spirulina powder of temperature；
C, 3% calcium chloride will be added in the spirulina protein after broken wall, is squeezed out brokenly by pneumatic cylinder automatic step
Protein liquid in wall spirulina protein, briquetting apparatus temperature are controlled at 0 DEG C or less；
D, the protein liquid pricked out is thawed at 0 DEG C, thawing time is 5~7h, and albumen is extracted in 5 DEG C of environment below
Liquid；
E, the protein liquid of extraction is purified by dissolved air flotation as shown in Figure 1 again after the filtering of Ultra filtration membrane system
Device extracts purity, and purity reaches 60% or more, wherein dissolved air flotation purifying plant include columned biochemical container 1,
The center of barostat 6, speed regulator 7 and recycling bin 4,1 top of biochemical container is installed by the charging with inlet valve 10
Pipe 2, biochemical container 1 are arranged the impurity communicated with recycling bin 4 and collect circle 5 outside, and it is in bending that impurity, which is collected and is uniformly distributed four groups on circle 5,
Shape and the impurity separating pipe 3 communicated with the top of biochemical container 1 are opened up on the inner wall of biochemical container 1 and are matched with impurity separating pipe 3
Diversion trench, the agitating device of installation connection speed adjuster 7 in biochemical container 1, agitating device is in biochemical container 1
Revolving vane 9, the number of revolving vane 9 is odd number, and the deflection angle of revolving vane 9 is 5 °, and the middle part of revolving vane 9 is installed
First rotating shaft, first rotating shaft are in hollow form, and 6 pipeline of barostat is connected in first rotating shaft inner cavity, pacifies on speed regulator 7
The second shaft is filled, driven wheel is installed in the lower section of first rotating shaft, and driving wheel is installed in the lower section of the second shaft, and driving wheel and driven wheel are logical
The connection of belt 8 is crossed, barostat 6 is connect with 1 pipeline of biochemical container, and wherein the pressure limit of barostat 6 is 3~5 public
The velocity interval of the pressure of jin, speed regulator 7 is 2300~2500r/min；
F, protein liquid after purification is obtained into the spirulina protein powder of about 35kg by high speed centrifugation drying machine drying,
Wherein drying temperature control is within 40 DEG C.
Embodiment 2
Difference lies in whipping temp is 15 DEG C to embodiment 2 in step B, mixing time 10h with embodiment 1.
Spirulina protein powder purity prepared by embodiment 1 is 70%, a concentration of 12g/L, spirulina egg prepared by embodiment 2
White powder purity is 75%, a concentration of 13g/L, and the spirulina protein powder that through the invention prepared by spirulina protein powder, preparation method thereof is pure
Degree and concentration are greatly improved, and entire production and purification process use it is a kind of based on dissolved air flotation purifying plant
The extraction of spirulina protein powder and the separation of light impurities are combined into one by physical method, the dissolved air flotation purifying plant, are simplified
Technological process, improves the service efficiency and disintegrate-quality of product.The spirulina protein powder prepared simultaneously by the preparation method
Natural sex, the safety that ensure that product, improve the content of spirulina protein in product, reduce production cost and period,
Reduce the waste of resource.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (7)
1. a kind of preparation method of spirulina protein powder, which is characterized in that include the following steps：
A, spirulina is crushed by pulverizer, obtains the spirulina powder that mesh number is 1200~1500 mesh, wherein pulverizer
Rotating speed is 5000~1000r/min, and grinding time is 10~15min；
B, the water that 8~10 times are added in the spirulina powder after crushing is stirred evenly, whipping temp is controlled at 15~20 DEG C, stirring
Time is 8~10h, and the spirulina powder fluid after uniform stirring, which is placed in high pressure homogenizer, carries out homogenize process, makes spirulina
Powder is swollen, ruptures, releasing the spirulina protein after broken wall；
C, 3% calcium chloride will be added in the spirulina protein after broken wall, broken wall spirulina protein is squeezed out by briquetting apparatus
In protein liquid, briquetting apparatus temperature control at 0 DEG C or less；
D, the protein liquid pricked out is thawed at 0~5 DEG C, thawing time is 5~7h, and protein liquid is extracted in 5 DEG C of environment below；
E, by the protein liquid of extraction by Ultra filtration membrane system filtering after again by dissolved air flotation purifying plant DNA purity, it is pure
Degree reaches 60% or more, and wherein dissolved air flotation purifying plant includes columned biochemical container, barostat, speed regulator
And recycling bin, the center at the top of biochemical container are installed the feed pipe with inlet valve, are arranged and recycling bin phase outside biochemical container
Logical impurity collects circle, and be uniformly distributed four groups on impurity collection circle detaches in bending and with the impurity communicated at the top of biochemical container
It manages, is opened up on the inner wall of biochemical container and the matched diversion trench of impurity separating pipe, the interior installation connection speed of biochemical container are adjusted
The agitating device of device, barostat are connect with biochemical container pipeline, and wherein the pressure limit of barostat is 3~5 kilograms
Pressure, the velocity interval of speed regulator is 2300~2500r/min；
F, protein liquid after purification is obtained into spirulina protein powder by high speed centrifugation drying machine drying.
2. the preparation method of spirulina protein powder as described in claim 1, which is characterized in that the processing of step B mesohigh homogenizers
The temperature of spirulina powder controls at 12 DEG C the albuminous degeneration hereinafter, in preventing temperature is excessively high from making spirulina powder.
3. the preparation method of spirulina protein powder as claimed in claim 2, which is characterized in that the briquetting apparatus in step C is gas
It takes offence cylinder, pneumatic cylinder automatically squeezes the protein liquid substep in spirulina protein.
4. the preparation method of spirulina protein powder as claimed in claim 3, which is characterized in that agitating device is installation in step E
The number of revolving vane in biochemical container, revolving vane is odd number, and the deflection angle of revolving vane is 5 °.
5. the preparation method of spirulina protein powder as claimed in claim 4, which is characterized in that the middle part of revolving vane in step E
First rotating shaft is installed, the second shaft is installed on speed regulator, driven wheel, the second shaft are installed below first rotating shaft
Lower section driving wheel is installed, driving wheel is connected with driven wheel by belt.
6. the preparation method of spirulina protein powder as claimed in claim 5, which is characterized in that first rotating shaft is in hollow in step E
Shape, barostat pipeline are connected in first rotating shaft inner cavity.
7. the preparation method of spirulina protein powder as claimed in claim 6, which is characterized in that the temperature in step F drying processes
Control is within 40 DEG C.
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| CN201810396433.1A CN108794565A (en) | 2018-04-27 | 2018-04-27 | A kind of preparation method of spirulina protein powder |
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| CN111728210A (en) * | 2020-06-17 | 2020-10-02 | 福建省神六保健食品有限公司 | Spirulina camel colostrum protein powder and processing technology thereof |
| CN114539349A (en) * | 2022-03-11 | 2022-05-27 | 青岛浩然海洋科技有限公司 | Extraction process of nostoc proteins |
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