CN102552376A - White kidney bean extract extracted by microwave as well as preparation method and application for same - Google Patents
White kidney bean extract extracted by microwave as well as preparation method and application for same Download PDFInfo
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- CN102552376A CN102552376A CN2010106227453A CN201010622745A CN102552376A CN 102552376 A CN102552376 A CN 102552376A CN 2010106227453 A CN2010106227453 A CN 2010106227453A CN 201010622745 A CN201010622745 A CN 201010622745A CN 102552376 A CN102552376 A CN 102552376A
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Abstract
A white kidney bean extract extracted by microwave is disclosed, wherein the bioactivity of an alpha-amylase inhibitor contained in the extract is equal to or higher than 4100 U/mg, and the HPLC (high-performance liquid chromatography) content is equal to or higher than 20% w/w. Preparation method for the extract is characterized by comprising the following steps of: crushing white kidney beans and passing through a 60-mesh sieve; preparing a mixture which is uniformly mixed by the crushed and sieved white kidney bean powder and purified water in a weight ratio of 1:5, extracting for 1-10 minutes at a temperature below 40 DEG C in microwave extraction equipment, then filtering to obtain a filtrate, and removing the filter residue; heating the filtrate to 70 DEG C in a water bath with stirring, keeping for 20 minutes, and collecting an effluent through a silica gel column; and drying the effluent to prepare the white kidney bean extract. The extract can be used for preparing an appetite inhibitor. The preparation method disclosed by the invention shortens extraction time and effectively increases extraction rate. The purified water is used as an extraction solvent, as well as is safe and environment-friendly.
Description
Technical field
The present invention relates to a kind of method for preparing of Semen Phaseoli Vulgaris extract, be specifically related to a kind of Semen Phaseoli Vulgaris extract with microwave extraction, belong to biological technical field.
Background technology
Semen Phaseoli Vulgaris is named as scarlet runner bean its biology, gains the name because of pattern is various.Belong to pulse family, Papillionoideae, Phaseoleae Phaseolus, formal name used at school are phaseolus coccineus L., the English Scarlet runner bean by name of P.multiflorus willd., Multiflora bean, white kidney bean.Semen Phaseoli Vulgaris originates in the torrid areas of South America, after introduce a fine variety in ground commerial growings such as Yunnan Province of China.Semen Phaseoli Vulgaris nutrition is very abundant, and protein content is higher than Carnis Gallus domesticus, and calcium content is more than 7 times of chicken, and ferrum is 4 times, and vitamin B group also is higher than Carnis Gallus domesticus.Semen Phaseoli Vulgaris still is a kind of rare high potassium, high magnesium, low-sodium diet simultaneously, and every hectogram contains 1520 milligrams in potassium, and 193.5 milligrams in magnesium, sodium are merely 0.8 milligram, especially is fit to heart disease, arteriosclerosis, hyperlipidemia, hypokalemia and avoids the salt edible for patients.The record of the ancient doctor of China mat, the Semen Phaseoli Vulgaris sweet in the mouth is flat, warm in nature, and have warming the middle warmer and descending qi, sharp the intestines and stomach, end functions such as singultus, kidney tonifying invigorating primordial QI, be a kind of nourishing dietetic therapy good merchantable brand.
Semen Phaseoli Vulgaris contains phaseolin (having another name called Semen Phaseoli Vulgaris albumen, English Phaseolin), is a kind of natural alpha-amylase inhibitor (α-amylase inhibitor, α-AI).Amylase inhibitor is a kind of pure-natural biological active substance, belongs to a kind of of glycosylhydrolase inhibitor, is present in the embryo of a plant seed Ruzhong, is referred to as " starch blocker " abroad.Alpha-amylase inhibitor can effectively suppress the decomposition of starch through to the inhibitory action of AMS, and then the heat that produces of blocking-up absorption of human body starch food and bring into play the fat-reducing effect; And excrete through gastrointestinal tract, do not get into blood circulation, do not act on big mesencephalic centre; In fat-reducing time, is appetite-suppressing not; Also have no side effect when using dosage is very high, very similar with the fat-reducing mechanism of Luo Shi orlistat, meet the fat-reducing principle of World Health Organization (WHO).Since the seventies in 20th century; Because of its at medically value alpha-amylase inhibitor by broad research; From plant seeds such as beans, Semen Tritici aestivi, wild Amaranthus, separated at present and obtained α-AI; Wherein active higher be the α-AI that from Semen Phaseoli Vulgaris, extracts, it has stronger inhibitory action to the mammal pancreatic, abroad it is used as slimming health food.
Be many methods that have that raw material extracts α-AI at present with the plant seed.Patent " purifying starch enzyme inhibitor and be used for obtaining the novel method of this amylase inhibitor " (CN1665521A) discloses a kind of impurity that utilizes supercritical carbon dioxide extraction to grind bean or pea; Be further purified the method for amylase inhibitor; Though the method step is simple, the supercritical extraction equipment investment is big, and condition is harsh; Heat time heating time is longer, can damage the activity of amylase inhibitor.Patent " a kind of method of from Kidney bean, extracting alpha-amylase inhibitor " (CN101269111A) discloses a kind of buffer ultrasonic extraction of using; Obtain the method for high-load alpha-amylase inhibitor through filtration, heating, centrifugal, dialysis, precipitate with ethanol, drying and other steps; The shortcoming of this method is that the production cycle is long; Process procedure is many, and heat time heating time, long resulting protease inhibitor product yield and activity were all lower.Patent " with the Semen Phaseoli Vulgaris extract of ethanol precipitation; its application and preparation " (CN101340924A) discloses and has a kind ofly made impurity and amylase inhibitor sedimentary method respectively with buffer extraction water and ethanol variable concentrations; The shortcoming of the method is the bad control of technology, and the content of product and activity are not high.
The alpha-amylase inhibitor of bibliographical information is to utilize traditional heating to extract mostly at present; Methods such as organic solvent deposit or inorganic salt deposition, these methods have the production cycle long, and energy consumption is high; Shortcomings such as contaminative is big, and product quality is low, cost is high, chemical residual is many.High-load alpha-amylase inhibitor product price is high in the industry Semen Phaseoli Vulgaris product at present; Do not take into account other nutritional labeling, the low content Semen Phaseoli Vulgaris extract of selling now on the market, phaseolin content has only about 1%; Mostly make through simple extraction concentrate drying method; Biological activity is poor, and taking dose is big, and health-care effect is not obvious.
Summary of the invention
The objective of the invention is in order to overcome weak point of the prior art, a kind of method that the microwave extraction technology prepares Semen Phaseoli Vulgaris extract of passing through is provided, the method preparation technology is simple, and cost is low, and is with short production cycle.This invention is applicable to the preparation appetite suppressant.
The technical problem that will solve required for the present invention, can realize through following technical scheme:
As first aspect of the present invention, a kind of Semen Phaseoli Vulgaris extract with microwave extraction is characterized in that the biological activity of the alpha-amylase inhibitor that said extract has is equal to or higher than 4100U/mg, and HPLC content is equal to or higher than 20%w/w.
As second portion of the present invention, a kind of method for preparing of the Semen Phaseoli Vulgaris extract with microwave extraction is characterized in that, may further comprise the steps:
(5) Semen Phaseoli Vulgaris was pulverized 60 mesh sieves;
(6) be the mixture that 1: 5 ratio is processed mix homogeneously with the Semen Phaseoli Vulgaris powder behind the crushing screening and pure water according to weight ratio, place Microwave Extraction Equipment to extract 1-10 minute below 40 ℃, filter then and obtain filtrating, filtering residue discards;
(7) above-mentioned filtrating is stirred heating in water bath to 70 ℃ maintenance 20 minutes, cross silicagel column and collect effluent;
(8) dry effluent makes Semen Phaseoli Vulgaris extract.
The power of the said Microwave Extraction Equipment of step (2) is: 1000-2000W, frequency: 2450MHz.
Mixture can extract at twice described in the productive rate step (2) in order to improve, and extracts 1-3 minute at every turn.
Filter press or centrifugal filtration are adopted in the said filtration of step (2).
In the said dry employing spray drying of step (4), decompression oven dry and the lyophilization one or more.
As third part of the present invention, a kind of application of the Semen Phaseoli Vulgaris extract with microwave extraction, said extract is used to prepare the food inhibitor.
Beneficial effect of the present invention:
(1) adopt the microwave extraction technology can extraction time of Semen Phaseoli Vulgaris be shortened to 1-10 minute from 5-8 hour of routine.
(2) because this technology extraction conditions is gentle; Heat time heating time is short; Compare commercially available Semen Phaseoli Vulgaris extract and kept the Semen Phaseoli Vulgaris original nutrient as far as possible, phaseolin HPLC content is greater than 20%, and biological activity is greater than 4100U/mg; Effect is much larger than the Semen Phaseoli Vulgaris extract of commercially available phaseolin 1% specification, and dose also reduces thereupon.
(3) this technology has only used pure water as extracting solvent in extracting rules, avoided in the existing Technology artificial introduce inorganic salt or organic solvent with problems such as the environmental pollution that causes, product quality and safety in utilization reductions.
(4) the phaseolin product that the present invention extracted has important use to be worth in medicine and field of health care products, is showing good prospects for application and economic worth aspect obesity, the diabetes especially, for being developed further into medicine and the health food raw material that provides the foundation.
Description of drawings
Further specify the present invention below in conjunction with the accompanying drawing and the specific embodiment.
Fig. 1 is a process chart of the present invention.
The drawing explanation:
Crushing screening 1, microwave extraction 2, filter 23, heat treated 4, mistake silicagel column 5 and dry 6 steps.
Semen Phaseoli Vulgaris 11, Semen Phaseoli Vulgaris powder 12, extracting solution 13, filtrating 14, treatment fluid 15, effluent 16, product 17.
The specific embodiment
In order to make technological means of the present invention, creation characteristic, to reach purpose and effect and be easy to understand and understand,, further set forth the present invention below in conjunction with specific embodiment.
A kind of Semen Phaseoli Vulgaris extract with microwave extraction, the biological activity of the alpha-amylase inhibitor that said extract has is equal to or higher than 4100U/mg, and HPLC content is equal to or higher than 20%w/w.The inhibition active testing of alpha-amylase inhibitor adopts iodine to be color method:
Utilize starch solution under the I-KI effect, to show blue; In certain starch concentration scope; The light absorption value at 660nm place and the linear characteristics of the amount of starch, the drawing standard curve can calculate the activity of inhibitor according to adding the difference of AMS starch-splitting amount before and after the inhibitor again.
The mensuration process is carried out in test tube.Getting 0.25mL concentration is the AMS of 1mg/mL with the phosphate buffer preparation, adds 0.25mL liquid to be measured, and 37 ℃ combine 30min.Add 1% soluble starch solution 1mL, add the iodine colour developing behind 37 ℃ of isothermal reaction 15min, measure absorbance in the 660nm place.Blank pipe does not add amylase and inhibitor, unconstrained dose of control tube.Difference according to absorbance is calculated the amount that suppresses to consume starch with standard curve, calculates the activity of inhibitor.
The inhibition of a unit active (U/mg) is that every milligram of inhibitor suppresses the amount (mg) that AMS consumes starch in 15min under these conditions.
Technological process of the present invention; As shown in Figure 1; Pass through crushing screening 1, microwave extraction 2, filter 23, heat treated 4, cross silicagel column 5 and dry 6 steps, Semen Phaseoli Vulgaris 11 → Semen Phaseoli Vulgaris powder 12 → extracting solution 13 → filtrating 14 → treatment fluid 15 → effluent 16 → product 17.
Embodiment 1:
The 100kg Semen Phaseoli Vulgaris is pulverized with pulverizer, cross behind 60 mesh sieves with pure water be that 1: 5 mixed is even according to weight ratio, place Microwave Extraction Equipment to extract twice below 40 ℃; Power: 1000W, frequency: 2450MHz, each 3 minutes; Filter, merging filtrate, filtering residue discards.To filtrate and stir heating in water bath to 70 ℃ maintenance 20 minutes, and at 30 ℃ of chromatographic columns of crossing the filling 200L silica gel of Φ 50 * 600cm, collect effluent, reuse 200L purified rinse water is collected effluent.Merge effluent, vacuum drying below 50 ℃ makes Semen Phaseoli Vulgaris extract 2.03kg, and the phaseolin activity is 4500U/mg, HPLC content: 23.2%W/W.The HPLC testing conditions is:
Instrument: SHIMADZU 20A series of high efficiency chromatograph of liquid; Post model: TSKgel, the full porous hydrophobic resin of Octadecyl-2PW (5 μ m, aperture 20nm), φ 4.6 * 150mm; Mobile phase: A (0.013mol/L HClO
4Acetonitrile solution), B (acetonitrile); Detector: UVD (280nm); Flow velocity: 1ml/min; The linear gradient elution program: in 15min, the mobile phase acetonitrile increases to 68% by 20%.
Embodiment 2:
The 100kg Semen Phaseoli Vulgaris is pulverized with pulverizer, cross behind 60 mesh sieves with pure water be that 1: 5 mixed is even according to weight ratio, place Microwave Extraction Equipment to extract twice below 40 ℃; Power: 2000W, each 1 minute of frequency: 2450MHz filters; Merging filtrate, filtering residue discards.To filtrate and stir heating in water bath to 70 ℃ maintenance 20 minutes, and at 30 ℃ of chromatographic columns of crossing the filling 200L silica gel of Φ 50 * 600cm, collect effluent, reuse 200L purified rinse water is collected effluent.Merge effluent, frozen drying makes Semen Phaseoli Vulgaris extract 1.82kg, and the phaseolin activity is 4100U/mg, HPLC content: 20.4%W/W.
More than show and described ultimate principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; The present invention is not restricted to the described embodiments; That describes in the foregoing description and the description just explains principle of the present invention; The present invention also has various changes and modifications under the prerequisite that does not break away from spirit and scope of the invention, and these variations and improvement all fall in the scope of the invention that requires protection.
Claims (7)
1. the Semen Phaseoli Vulgaris extract with microwave extraction is characterized in that, the biological activity that said extract has active alpha-amylase inhibitor is equal to or higher than 4100U/mg, and HPLC content is equal to or higher than 20%w/w.
2. the method for preparing of the Semen Phaseoli Vulgaris extract of the usefulness microwave extraction shown in claim 1 is characterized in that, may further comprise the steps:
(1) Semen Phaseoli Vulgaris was pulverized 60 mesh sieves;
(2) be the mixture that 1: 5 ratio is processed mix homogeneously with the Semen Phaseoli Vulgaris powder behind the crushing screening and pure water according to weight ratio, place Microwave Extraction Equipment to extract 1-10 minute below 40 ℃, filter then and obtain filtrating, filtering residue discards;
(3) above-mentioned filtrating is stirred heating in water bath to 70 ℃ maintenance 20 minutes, cross silicagel column and collect effluent;
(4) dry effluent makes Semen Phaseoli Vulgaris extract.
3. method for preparing according to claim 2 is characterized in that, in the step (2), the power of said Microwave Extraction Equipment is: 1000-2000W, frequency: 2450MHz.
4. method for preparing according to claim 2 is characterized in that, in the step (2), said mixture extracts at twice, extracts 1-3 minute at every turn.
5. method for preparing according to claim 2 is characterized in that, in the step (2), filter press or centrifugal filtration are adopted in said filtration.
6. method for preparing according to claim 2 is characterized in that, in the step (4), and one or more in said dry employing spray drying, decompression oven dry and the lyophilization.
7. the application with the Semen Phaseoli Vulgaris extract of microwave extraction is characterized in that, said extract is used to prepare appetite suppressant.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169024A (en) * | 2013-04-17 | 2013-06-26 | 黑龙江八一农垦大学 | Method for extracting kidney bean starch and coproducing kidney bean protein powder and dietary fiber powder |
| CN103638089A (en) * | 2013-11-29 | 2014-03-19 | 广东省食品工业研究所 | Method for extracting alpha-amylase inhibitor from white kidney beans by use of microwave-compound enzyme coupling method |
| CN104770737A (en) * | 2015-03-25 | 2015-07-15 | 陕西鑫茂生物科技有限公司 | Phaseolus vulgaris alpha-amylase inhibitor extraction and separation method |
| CN105747231A (en) * | 2016-03-16 | 2016-07-13 | 宝健（北京）生物技术有限公司 | Technology for extracting and preparing starch inhibitor from white kidney beans |
| WO2017168045A1 (en) * | 2016-03-31 | 2017-10-05 | Kemira Oyj | A method and system for determination of starch in a sample |
| CN110250436A (en) * | 2019-06-13 | 2019-09-20 | 富民嘉友食品有限公司 | A kind of control energy mung bean cake |
| CN112971137A (en) * | 2019-12-13 | 2021-06-18 | 黔东南苗族侗族自治州农业科学院 | Extraction method of black kidney bean extract |
| CN115251373A (en) * | 2022-08-12 | 2022-11-01 | 中科爱伽(天津)医用食品有限公司 | Special dietary food for controlling weight, preparation process and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101269111A (en) * | 2008-05-09 | 2008-09-24 | 陕西卓瑞生物技术有限公司 | Method for extracting alpha-diastase restrainer from phaseolus limensis |
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2010
- 2010-12-31 CN CN2010106227453A patent/CN102552376A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101269111A (en) * | 2008-05-09 | 2008-09-24 | 陕西卓瑞生物技术有限公司 | Method for extracting alpha-diastase restrainer from phaseolus limensis |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169024A (en) * | 2013-04-17 | 2013-06-26 | 黑龙江八一农垦大学 | Method for extracting kidney bean starch and coproducing kidney bean protein powder and dietary fiber powder |
| CN103169024B (en) * | 2013-04-17 | 2014-03-26 | 黑龙江八一农垦大学 | Method for extracting kidney bean starch and coproducing kidney bean protein powder and dietary fiber powder |
| CN103638089A (en) * | 2013-11-29 | 2014-03-19 | 广东省食品工业研究所 | Method for extracting alpha-amylase inhibitor from white kidney beans by use of microwave-compound enzyme coupling method |
| CN103638089B (en) * | 2013-11-29 | 2016-01-27 | 广东省食品工业研究所 | A kind of method utilizing microwave-compound enzyme coupled method to extract alpha-amylase inhibitor from Semen Phaseoli Vulgaris |
| CN104770737A (en) * | 2015-03-25 | 2015-07-15 | 陕西鑫茂生物科技有限公司 | Phaseolus vulgaris alpha-amylase inhibitor extraction and separation method |
| CN105747231A (en) * | 2016-03-16 | 2016-07-13 | 宝健（北京）生物技术有限公司 | Technology for extracting and preparing starch inhibitor from white kidney beans |
| WO2017168045A1 (en) * | 2016-03-31 | 2017-10-05 | Kemira Oyj | A method and system for determination of starch in a sample |
| TWI740917B (en) * | 2016-03-31 | 2021-10-01 | 芬蘭商克密拉股份有限公司 | A method and system for determination of starch in a sample |
| CN110250436A (en) * | 2019-06-13 | 2019-09-20 | 富民嘉友食品有限公司 | A kind of control energy mung bean cake |
| CN112971137A (en) * | 2019-12-13 | 2021-06-18 | 黔东南苗族侗族自治州农业科学院 | Extraction method of black kidney bean extract |
| CN115251373A (en) * | 2022-08-12 | 2022-11-01 | 中科爱伽(天津)医用食品有限公司 | Special dietary food for controlling weight, preparation process and application thereof |
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Application publication date: 20120711 |