CN112535239B - Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof - Google Patents
Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof Download PDFInfo
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- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P39/06—Free radical scavengers or antioxidants
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and a preparation method thereof, which is prepared from raw materials of 10-20 parts of isatis root enzymolysis polysaccharide, 1-2 parts of sophora alopecuroide alkaloid, 5-15 parts of salvia miltiorrhiza residue fermentation polysaccharide, 5-10 parts of shaddock peel polysaccharide and 5-15 parts of honeysuckle stem polysaccharide. Experiments show that the compound Chinese herbal medicine feed additive can enhance the appetite of animals, promote the growth of the animals and improve the immunity of the animal bodies. The invention has the characteristics of environment-friendly materials, low cost, innovative process, good practicability and the like, and can improve the autoimmune function of animals, balance the microecology of gastrointestinal tracts and form excellent health care effect when being added into daily feed for breeding, thereby reducing the morbidity of the animals, reducing the medication and even avoiding the use of the animals in the breeding process, improving the feed rewarding, reducing the feed conversion ratio, improving the growth speed of the animals, ensuring the quality of the products, enhancing the competitiveness and improving the economic benefit of farmers (farms).
Description
Technical Field
The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a compound Chinese herbal medicine feed additive for promoting growth and enhancing immunity and daily health care of livestock and a preparation method thereof.
Background
In the livestock industry, antibiotics are used as feed additives, and are abused in feed for a long time and in a large quantity, so that excessive residues of the antibiotics in animal foods are caused, and the food safety and human health are greatly damaged. From 7 months and 1 day in 2020, the feed producer stops producing commercial feeds containing growth-promoting medicinal feed additives other than traditional Chinese medicines. The traditional Chinese veterinary medicine has the advantages of resisting viruses, improving immunity, resisting stress, promoting growth and development and the like, so that the traditional Chinese veterinary medicine is widely paid attention to as a medicine for preventing and treating diseases and promoting animal growth.
The current market demand for veterinary drugs is mainly focused on the following categories: first, traditional Chinese medicines with antibacterial effect, such as radix Scutellariae, rhizoma Coptidis, herba Houttuyniae, herba Andrographitis, etc.; secondly, the traditional Chinese medicine for promoting animal growth is mainly used for helping digestion and strengthening spleen, such as hawthorn, malt, dried orange peel and the like, and tonifying middle-jiao and Qi of the formula of the traditional Chinese medicine, such as bighead atractylodes rhizome, liquorice, rhizoma polygonati and the like; thirdly, the traditional Chinese medicines with the function of expelling parasites, such as rangooncreeper fruit, cyrtomium rhizome, betel nut and the like. At present, the main problems in the research and development of traditional Chinese veterinary medicines are as follows: firstly, the prescription is based on the theory of traditional Chinese medicine, and the compatibility of medicines is little; secondly, the new dosage forms are few, most of the new dosage forms are water extraction or crude drug crushing is directly used, the new preparation is few, and the research on bioavailability of the effective components of the traditional Chinese medicine is few; thirdly, in the research and development of traditional Chinese veterinary medicines, the vast majority of the traditional Chinese medicines are directly prepared by adopting raw medicines, the preparation process is simple, the preparation cost is high, and the waste of the traditional Chinese herbal medicine resources is easily caused. Therefore, a feed additive product with low cost, good effect and refinement is needed in the market.
The radix isatidis is a large variety of traditional Chinese medicinal materials commonly used in China, and the radix isatidis polysaccharide has the effects of resisting virus, resisting oxidation, resisting inflammation, inhibiting bacteria, resisting tumor, regulating immunity and the like. The traditional isatis root preparation process adopts a water extraction and alcohol precipitation method, and the isatis root polysaccharide accounting for more than one fourth of the total mass is discarded as residue, so that the isatis root polysaccharide after alcohol precipitation has larger molecular weight and extremely poor solubility, and polysaccharide fragments with smaller molecular weight are obtained by carrying out enzymolysis on the isatis root polysaccharide fragments through biological enzymes, so that the water solubility and bioavailability of the isatis root polysaccharide fragments are increased, and the isatis root polysaccharide can be used as a high-quality animal feed additive.
The sophora alopecuroide is a leguminous plant, has the characteristic of high nutritive value, and is a potential pasture resource. The analysis result shows that the sophora alopecuroide alkaloid component has pharmacological effects of resisting tumor, inhibiting bacteria, resisting virus, easing pain, resisting convulsion, resisting arrhythmia, improving myocardial ischemia, reducing blood fat and the like. Therefore, the sophora alopecuroide and the sophora alopecuroide residues can improve the immunity of animals, and are high-quality supplementary feed.
Modern pharmacological researches show that the radix salviae miltiorrhizae has stronger pharmacological effects in various aspects of bacteriostasis, anti-inflammation, antioxidation, anti-tumor, anti-liver cirrhosis, anti-fibrosis, heart protection, neuroprotection, immunoregulation and the like, and the pressure of the dregs of the radix salviae miltiorrhizae on the ecological environment is increasingly severe due to the large-scale use of the radix salviae miltiorrhizae, and the dregs of the radix salviae miltiorrhizae which is secondarily recovered by the effective components are subjected to multi-strain mixed fermentation again, so that abundant components which are difficult to be utilized such as cellulose, lignin and the like can be converted into soluble fermentation polysaccharide for producing biological feed.
The pomelo is one of the most commonly eaten fruits in daily life, the pomelo peel is the outermost dry peel of the pomelo which is not fully mature or is fully mature, the ratio of the pomelo peel to the whole pomelo is 30% -40%, and the pomelo peel polysaccharide can enhance the immunity of organisms, resist inflammation, viruses and oxidization, has a certain preventive effect on tumors, and can also regulate the blood sugar and blood fat of organisms and the like. The discarded shaddock peel is reprocessed and utilized, and added into animal feed, so that the shaddock peel has good palatability, and can promote growth and improve animal immunity.
The dried flower bud of the honeysuckle stem is directly used as a raw material for industrially extracting active ingredients, so the demand is quite large, and especially in the period of epidemic outbreak, the situation of supply shortage often occurs. Compared with the honeysuckle, the development and utilization of the honeysuckle stem are not as extensive as that of the honeysuckle, the pharmacological action of the honeysuckle stem polysaccharide is similar to that of the honeysuckle, and the honeysuckle stem polysaccharide has the effects of resisting bacteria, resisting viruses, resisting inflammation, resisting oxidation, protecting liver, benefiting gall, resisting tumor and the like, has certain influence on the immune system and the cardiovascular system, and can achieve 9.05 percent of polysaccharide yield by utilizing ultrasonic wave for auxiliary extraction of the polysaccharide in the honeysuckle stem, thereby having great feeding value.
The feed additive prepared by the invention is a compound composed of a plurality of components, has various functions, can inhibit or kill pathogens of infectious diseases, and can enhance the nonspecific immunity of animal organisms, thereby reducing the incidence of certain infectious diseases, improving and regulating intestinal flora of animals and promoting appetite of animals. And has wide sources and low cost. Compared with the traditional antibiotic antibacterial antiviral drugs, the drug has small toxic and side effects and no drug resistance, can not increase the tolerance of bacteria to the drugs after long-term use, and can prevent the multi-resistance bacteria generated by abuse of antibiotics from being transferred through food chains, thereby bringing potential harm to human beings.
Disclosure of Invention
The invention provides a compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock.
The invention provides a compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock, which is prepared from the following raw materials in parts by weight:
10-20 parts of isatis root enzymolysis polysaccharide; 1-2 parts of sophora alopecuroide alkaloid; 5-15 parts of salvia miltiorrhiza residue fermentation polysaccharide; 5-10 parts of shaddock peel polysaccharide; 5-15 parts of honeysuckle stem polysaccharide.
As a preferable technical scheme of the invention, each traditional Chinese medicine of the compound Chinese herbal medicine feed additive (formula one) for daily health care and growth promotion of livestock is specifically as follows: 10 parts of isatis root enzymolysis polysaccharide; 1 part of sophora alopecuroide alkaloid; 10 parts of salvia miltiorrhiza residue fermentation polysaccharide; 5 parts of shaddock peel polysaccharide; 5 parts of honeysuckle stem polysaccharide, and the total polysaccharide content is 78.30 percent.
As a preferable technical scheme of the invention, each traditional Chinese medicine of the compound Chinese herbal medicine feed additive (formula II) for daily health care and growth promotion of livestock is specifically as follows: 15 parts of isatis root enzymolysis polysaccharide; 2 parts of sophora alopecuroide alkaloid; 15 parts of salvia miltiorrhiza residue fermentation polysaccharide; 10 parts of shaddock peel polysaccharide; 10 parts of honeysuckle stem polysaccharide, and the total polysaccharide content is measured to be 81.73 percent.
As a preferable technical scheme of the invention, each traditional Chinese medicine of the compound Chinese herbal medicine feed additive (formula III) for daily health care and growth promotion of livestock is specifically as follows: 20 parts of isatis root enzymolysis polysaccharide; 1 part of sophora alopecuroide alkaloid; 15 parts of salvia miltiorrhiza residue fermentation polysaccharide; 10 parts of shaddock peel polysaccharide; 10 parts of honeysuckle stem polysaccharide, and the total polysaccharide content is 89.41 percent.
Three compound Chinese herbal medicine feed additives are prepared, and the total polysaccharide content obtained by the third formula is highest.
The compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock is prepared by a method comprising the following steps:
(1) Extracting radix Isatidis crude polysaccharide with ethanol, collecting precipitate, volatilizing ethanol in the precipitate, and lyophilizing to obtain radix Isatidis refined polysaccharide; dissolving the purified polysaccharide in water, performing enzymolysis, cooling, centrifuging, collecting supernatant, concentrating under reduced pressure to obtain thick paste extract, and vacuum drying to obtain radix Isatidis enzymolysis polysaccharide;
(2) Removing impurities from herba Sophorae Alopecuroidis, pulverizing, ultrasonic extracting, filtering, and concentrating the filtrate under reduced pressure; dissolving the extract with appropriate amount of acid water to obtain acid water solution, adsorbing with macroporous adsorbent resin, collecting effluent, concentrating under reduced pressure, and vacuum drying to obtain herba Sophorae Alopecuroidis alkaloid extract;
(3) Collecting Saviae Miltiorrhizae radix water extract residue, recovering liposoluble components with ethanol, oven drying, placing in culture medium, fermenting with medicinal fungus, and drying with low temperature air blast to obtain Saviae Miltiorrhizae radix residue fermentation polysaccharide;
(4) Taking coarse powder of shaddock peel, carrying out enzymolysis by using compound enzyme, carrying out constant-temperature shaking, carrying out suction filtration, collecting filtrate, evaporating and concentrating, and cooling; adding absolute ethyl alcohol for dilution, standing, and carrying out suction filtration to obtain a filter cake, namely shaddock peel polysaccharide;
(5) Pulverizing caulis Lonicerae, sieving, adding distilled water, heating, ultrasonic extracting, vacuum filtering, concentrating the filtrate by rotary evaporation, precipitating with ethanol, standing overnight, centrifuging the filtrate, dissolving the precipitate with ultrapure water, separating with ceramic membrane, collecting the permeate, concentrating, and vacuum drying to obtain caulis Lonicerae polysaccharide;
(6) Mixing radix Isatidis enzymolysis polysaccharide, herba Sophorae Alopecuroidis alkaloid, saviae Miltiorrhizae radix residue fermentation polysaccharide, pericarpium Citri Grandis polysaccharide and caulis Lonicerae polysaccharide with sucrose and dextrin, and grinding into fine powder to obtain feed additive.
In the step (1), the crude polysaccharide of the isatis root is taken, and the liquid medicine ratio is 1: 5-6, adding ethanol with the volume concentration of 60-80%, carrying out cold leaching extraction for 4-8 hours at the temperature of 4 ℃, collecting precipitate, volatilizing the ethanol in the obtained precipitate, and freeze-drying to obtain the isatis root refined polysaccharide; dissolving isatis root refined polysaccharide in water, adding cellulase, pectase or alpha-amylase (bacillus subtilis) for enzymolysis, wherein the enzyme activity is 100-500U/mL, carrying out enzymolysis for 3-7 hours at 40-60 ℃, heating with boiling water for 15-30 min, cooling and centrifuging, collecting supernatant, concentrating under reduced pressure to obtain thick paste extract, and carrying out vacuum drying to obtain isatis root enzymolysis polysaccharide;
in the step (2), the bitter beans are takenRemoving impurities and crushing seeds, wherein the liquid medicine ratio is 1: 4-6, adding ethanol with the volume concentration of 50-70%, performing ultrasonic extraction for 1.5-3 hours, filtering, and concentrating the filtrate under reduced pressure to obtain an extract; dissolving the extract with an appropriate amount of acid water to obtain an acid water solution, and regulating the pH to 10-13 with 50g/L sodium hydroxide solution, wherein the pH is 4500-5000 r.min -1 Centrifuging, taking supernatant, and adding 0.5-1.0 mol/LNaCl solution with equal volume to obtain adsorption liquid; the macroporous adsorption resin is selected from NKA-12 resin, X-5 resin, DF01 resin or DA101 resin, the resin is fully swelled and then is filled into a glass column, the adsorption liquid passes through the adsorption resin column from top to bottom, the adsorption temperature is 15-25 ℃, the ethanol eluent with the volume concentration of 70% is taken as the eluent, and the adsorption flow rate is 2-8 BV/h; collecting effluent, concentrating under reduced pressure, and vacuum drying to obtain herba Sophorae Alopecuroidis alkaloid;
in the step (3), after the fat-soluble components are recovered by using ethanol with the volume concentration of 70-100%, the liquid medicine ratio is 1: 8-10, and refluxing for 3-3.5 hours at a temperature of 55-70 ℃; oven drying, placing in culture medium, fermenting with medicinal fungus, wherein the fermentation strain can be Ganoderma lucidum G1, ganoderma lucidum G8 and Poria cocos P01, the inoculation amount is 10-20%, the temperature is 20-35deg.C, and the ratio of Saviae Miltiorrhizae radix to testa Tritici is 3: 1-1.2; air-drying at 50-60 ℃ to obtain salvia miltiorrhiza residue fermentation polysaccharide;
in the step (4), the coarse powder of the shaddock peel is taken, and the mass ratio of the coarse powder to the shaddock peel is 1: 0.5-1.5 of cellulase and pectinase, wherein the enzyme dosage is 1.0-5.0%, and disodium hydrogen phosphate citric acid buffer solution with the pH value of 4.0-6.0 is added, and the feed liquid ratio is 1: 25-35, wherein the extraction temperature is 40-60 ℃ and the extraction time is 60-80 min; shaking at constant temperature, filtering, collecting filtrate, evaporating, concentrating, and cooling; adding absolute ethyl alcohol for dilution, wherein the addition amount is 1.5-3.5 times of that of the concentrated solution, standing for 30-50 min, and carrying out suction filtration to obtain a filter cake, namely shaddock peel polysaccharide;
in the step (5), the honeysuckle stem is crushed and sieved, the granularity is 50-65 meshes, distilled water is added, and the liquid-material ratio is 30: 1-1.2, heating and ultrasonic extraction, wherein the extraction time is 30-50 min, the ultrasonic power is 180-220W, and the extraction temperature is 60-70 ℃; filtering, concentrating the filtrate by rotary evaporation, precipitating with ethanol overnight, centrifuging the filtrate, dissolving the precipitate with ultrapure water, separating by a ceramic membrane, wherein the ceramic membrane can be UF-100, UF-50, UF-10 or UF-5, the feed flow is 25-40 mL/min, the ultrafiltration temperature is 40-50 ℃, and the operation pressure is 0.15-0.2 MPa; collecting the permeate, concentrating, and vacuum drying to obtain caulis Lonicerae polysaccharide;
the mass ratio of the isatis root enzymolysis polysaccharide, the sophora alopecuroide alkaloid, the salvia miltiorrhiza residue fermentation polysaccharide, the shaddock peel polysaccharide, the honeysuckle stem polysaccharide, the sucrose and the dextrin in the step (6) is 10-20: 1-2: 5-15: 5-10: 5-15: 10:10.
the application of the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock serving as a traditional Chinese veterinary medicine in promoting animal growth, enhancing immunity and improving antibacterial and antiviral capabilities also belongs to the protection scope of the invention.
The research shows that the isatis root enzymolysis polysaccharide, the sophora alopecuroide alkaloid, the salvia miltiorrhiza residue fermentation polysaccharide and the shaddock peel polysaccharide are used for preparing the animal feed additive, so that the animal growth can be promoted and the immunity can be enhanced. The compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock, provided by the invention, has the effects of promoting growth, enhancing immunity and improving antibacterial and antiviral capabilities, and creates more economic and social values.
The compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock is prepared from Chinese herbal medicine residue extracts of isatis root enzymolysis polysaccharide, sophora alopecuroide alkaloid, salvia miltiorrhiza residue fermentation polysaccharide, shaddock peel polysaccharide, honeysuckle stem polysaccharide and the like serving as raw materials. The invention has the advantages of low cost, growth promotion, feed utilization rate improvement, organism immunity improvement, oxidation stress resistance enhancement and the like.
The invention adopts a method of combining traditional Chinese medicine and livestock and poultry industry, combines medication experience under the guidance of traditional Chinese medicine theory, combines isatis root enzymolysis polysaccharide, sophora alopecuroide alkaloid, red-rooted salvia root residue fermentation polysaccharide, shaddock peel polysaccharide and honeysuckle stem polysaccharide into a compound, is applied to livestock and poultry feed industry, produces functional feed additives, can improve the immunity of animals, has the effects of promoting growth, enhancing immunity and improving feed utilization rate, and has no toxic or side effect. The development of the traditional Chinese medicine compound feed expands the application field of traditional Chinese medicine compound and provides a safe and reliable traditional Chinese medicine compound feed additive product for the market.
The invention is based on the traditional Chinese medicine theory to carry out the formulation, the radix isatidis is used as a monarch drug in the feed medicine, can enhance the immunity, is matched with other drugs, and is prepared from a plurality of effects of promoting growth, improving the immunity and the like, and the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock is developed, the naturalness and the biological activity of the effective components of the traditional Chinese medicine are reserved, and the effective components are easy to absorb and utilize, so that the nonspecific immunity of an organism is mainly improved and promoted, the immunity and the disease resistance of the organism of an animal are enhanced, and the forward regulation of the oxidation resistance and the like is enhanced. The effective components are enriched to prepare granules, which is favorable for storage and compound mixing with feed. The effective extraction process is selected to improve the utilization rate of the effective components and achieve the aim of synergy.
Detailed Description
The experimental methods used in the following examples are all conventional methods unless otherwise specified; the reagents, materials, etc. used in the examples described below are commercially available unless otherwise specified.
Example 1
1. The compound Chinese herbal medicine feed additive comprises the following components: 20 parts of isatis root enzymolysis polysaccharide, 1 part of sophora alopecuroide alkaloid, 15 parts of salvia miltiorrhiza residue fermentation polysaccharide, 10 parts of shaddock peel polysaccharide and 10 parts of honeysuckle stem polysaccharide.
2. The preparation process comprises the following steps:
(1) Taking the isatis root crude polysaccharide, wherein the liquid medicine ratio is 1:5, adding ethanol with the volume concentration of 60%, cold soaking and extracting at 4 ℃ for 6 h, collecting precipitate, volatilizing the ethanol in the obtained precipitate, and freeze-drying to obtain the isatis root refined polysaccharide; dissolving radix Isatidis refined polysaccharide in water, adding cellulase for enzymolysis, performing enzymolysis at 40deg.C for 4 h at 200U/mL, heating with boiling water for 15 min, cooling, centrifuging, collecting supernatant, concentrating under reduced pressure to obtain soft extract, and vacuum drying to obtain radix Isatidis enzymolysis polysaccharide;
(2) Taking sophora alopecuroide, removing impurities and crushing, wherein the medicine liquid ratio is 1:5, adding ethanol with the volume concentration of 70% for ultrasonic extraction of 1.5 and h, filtering, and concentrating the filtrate under reduced pressure to obtain extract; by usingDissolving the extract with appropriate amount of acid water to obtain acid water solution, adjusting pH to 10 with 50g/L sodium hydroxide solution, and adjusting pH to 5000 r min -1 Centrifuging, taking supernatant, and adding an equal volume of 1.0mol/L NaCl solution to obtain adsorption liquid; preparing macroporous adsorption resin, namely selecting DF01 resin, fully swelling the resin, filling the resin into a glass column, enabling the resin to pass through an adsorption liquid from top to bottom, eluting by using ethanol with the volume concentration of 70% as an eluent, wherein the elution adsorption flow rate is 4 BV/h; collecting effluent, concentrating under reduced pressure, and vacuum drying to obtain herba Sophorae Alopecuroidis alkaloid;
(3) Taking the water extraction residues of the red sage root according to the liquid medicine ratio of 1:8, recovering fat-soluble components by refluxing 3 h at 55 ℃ with 70% ethanol, drying, placing in a culture medium, fermenting with medicinal fungus, wherein the fermentation strain is Ganoderma lucidum G1, the inoculation amount is 10%, the temperature is 20 ℃, and the ratio of radix Salviae Miltiorrhizae to testa Tritici is 3:1, a step of; air drying at 60deg.C to obtain Saviae Miltiorrhizae radix residue fermented polysaccharide;
(4) Taking coarse powder of shaddock peel, and adding the following components in percentage by mass: 1.5 cellulase and pectase, the enzyme dosage is 3.0%, and disodium hydrogen phosphate citric acid buffer solution with pH value of 4.0 is added, the feed liquid ratio is 1:30, the extraction temperature is 40 ℃, and the extraction time is 60 min; shaking at constant temperature, filtering, collecting filtrate, evaporating, concentrating, and cooling; adding absolute ethyl alcohol for dilution, wherein the adding amount is 1.5 times of that of the concentrated solution, standing for 30 min, and performing suction filtration to obtain a filter cake, namely shaddock peel polysaccharide;
(5) Pulverizing caulis Lonicerae, sieving with 65 mesh sieve, adding distilled water, and mixing with liquid material at a ratio of 30:1, heating ultrasonic extraction for 30 min, wherein the ultrasonic power is 180W, and the extraction temperature is 60 ℃; suction filtering, rotary evaporating and concentrating the filtrate, precipitating with ethanol overnight, centrifuging the filtrate, dissolving the precipitate with ultrapure water, separating with ceramic membrane UF-50 with feed flow of 25 mL/min, ultrafiltration temperature of 50deg.C and operation pressure of 0.2 MPa; collecting the permeate, concentrating, and vacuum drying to obtain caulis Lonicerae polysaccharide;
(6) Mixing and grinding 20 parts of isatis root enzymolysis polysaccharide, 1 part of sophora alopecuroide alkaloid, 15 parts of salvia miltiorrhiza residue fermentation polysaccharide, 10 parts of shaddock peel polysaccharide, 10 parts of honeysuckle stem polysaccharide, 10 parts of sucrose and 10 parts of dextrin into uniform fine powder to obtain the feed additive.
Example 2
1. The compound Chinese herbal medicine feed additive comprises the following components:
10 parts of isatis root enzymolysis polysaccharide; 1 part of sophora alopecuroide alkaloid; 10 parts of salvia miltiorrhiza residue fermentation polysaccharide; 5 parts of shaddock peel polysaccharide; 5 parts of honeysuckle stem polysaccharide.
2. The preparation process comprises the following steps:
step (1) to step (5) are the same as in example 1;
step (6): mixing and grinding 10 parts of isatis root enzymolysis polysaccharide, 1 part of sophora alopecuroide alkaloid, 10 parts of salvia miltiorrhiza residue fermentation polysaccharide, 5 parts of shaddock peel polysaccharide, 5 parts of honeysuckle stem polysaccharide, 10 parts of sucrose and 10 parts of dextrin into uniform fine powder to obtain the feed additive.
Example 3
1. The compound Chinese herbal medicine feed additive comprises the following components:
15 parts of isatis root enzymolysis polysaccharide; 2 parts of sophora alopecuroide alkaloid; 15 parts of salvia miltiorrhiza residue fermentation polysaccharide; 10 parts of shaddock peel polysaccharide; 10 parts of honeysuckle stem polysaccharide.
2. The preparation process comprises the following steps:
step (1) to step (5) are the same as in example 1;
step (6): 15 parts of isatis root enzymolysis polysaccharide; 2 parts of sophora alopecuroide alkaloid; 15 parts of salvia miltiorrhiza residue fermentation polysaccharide; 10 parts of shaddock peel polysaccharide; 10 parts of honeysuckle stem polysaccharide, 10 parts of sucrose and 10 parts of dextrin are mixed and ground into uniform fine powder, and the feed additive is obtained.
Example 4 safety evaluation acute toxicity test
1. Experimental animals and reagents: healthy SPF-grade BALB/c mice, male and female halves, body mass (18.0.+ -. 2.0) g, purchased from Shanghai Jie laboratory animal Co., ltd., quality eligibility certificate: SCXK 2018-0004. The conventional cage-separating feeding is carried out, the free drinking water is used for feeding, the indoor temperature is controlled at 21-24 ℃, the humidity is 30-50%, and the air is circulated. Test drug: the feed additive prepared in example 1.
2. The implementation process comprises the following steps: the male and female animals were grouped by body weight, and were fasted for 12 h in the evening before the day, and were not forbidden to be watered, and the administration concentration was the maximum (2.98 g/mL), (which means that the crude drug contained in 1mL was 2.98 g, namely 298 g was extracted and concentrated to 100 mL,1mL was 2.98 g crude drug), and the administration volume (0.3 mL/10 g) was lavaged 3 times a day, and after the completion of the lavage, 1 h was restored to food, and the condition of the mice was observed, as shown in Table 1.
The results show that: after 3 times of gastric lavage a day, animals do not die, and no abnormality is observed to external reaction, appearance, behavior, respiration and the like, and no obvious poisoning symptom is observed. No obvious abnormal symptoms (fur, respiration, eyes, mucous membrane, behavior) were observed from day one to day 14 after administration. After 14 days, the results show that: no obvious lesions are found in heart, liver, spleen, lung, kidney, brain, thymus, sexual organs, etc.
TABLE 1 weight change in mice [ (]
）
| Group of | Day 1 | Day 2 | Day 5 | Day 8 | Day 11 | Day 14 |
| Female group | 22.28±2.27 | 22.54±2.34 | 23.51±2.21 | 24.31±1.93 | 25.84±2.20 | 25.81±2.56 |
| Male group | 22.61±2.01 | 22.97±2.11 | 23.87±2.00 | 25.70±1.97 | 27.4±1.20 | 27.66±1.42 |
EXAMPLE 5 quality Standard study
Test drug: the feed additive prepared in example 1.
Reagent: maltodextrin (Shandong West Wang Tangye Co., ltd., GB/I20884), ethanol (analytical grade, tianjin Yongda chemical Co., ltd.), phenol, concentrated sulfuric acid (analytical grade, guangzhou chemical Co., ltd.), vanillin (analytical grade, nanjing regular triangle chemical Co., ltd.).
Instrument: pharmacopoeia sieves, dryers, weighing bottles, UV-6100S ultraviolet visible spectrophotometers (Shanghai Yuan analytical instruments Co., ltd.), AX523ZH analytical balance (Orhaus instruments Co., ltd.), EX225DZH analytical balance (Orhaus instruments Co., ltd.), BSG-12 timing constant temperature water bath (Kingjie JieRuer electric Co., ltd.), DHG-9145A vertical electric constant temperature blast drying oven (Shanghai ring race test Equipment Co., ltd.), JIDI-20D bench type multipurpose high speed centrifuge (Guangzhou Ji Di instruments Co., ltd.), JIDI-5D bench type high capacity low speed centrifuge (Guangzhou Ji Di instruments Co., ltd.).
Results of trait analysis: the product is a kind of soluble granule which is yellow brown, has no special smell, and has the advantages of dry and uniform granule, and no phenomena of moisture absorption, caking, deliquescence, etc.
Granularity inspection results: 6.22g of compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock is weighed, after sieving, 0.0 g of No. 1 sieve cannot pass, 1.07 g of No. 5 sieve cannot pass, and the result shows that the total percentage of the No. 1 sieve to the No. five sieve cannot pass is 17.20%.
Solubility check results: the compound Chinese herbal medicine feed additive 10 g for daily health care and growth promotion of livestock is taken, heated water is 200 mL, stirred for 5 minutes, and immediately observed, the soluble particles should be completely dissolved or slightly turbid. The compound Chinese herbal medicine feed additive 14.9. 14.9 g for daily health care and growth promotion of livestock is weighed, water is heated and stirred, slight turbidity is generated, and the solubility meets the regulations.
Moisture measurement results: the water content is 3.24%, 2.73% and 3.46% respectively, and is less than 8% by weight for three times.
Example 6 efficacy experiment on broiler chickens
1. Influence on the growth of broiler chickens
The compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock obtained in the examples 1-3 is adopted for carrying out relevant experimental comparison.
100 feather Ross 308 broilers with 1 day old are selected, male and female chickens are mixed, and after 7d of adaptive feeding, the broilers are randomly divided into 4 treatments according to the principle of no difference of repeated weights, each group is divided into 5 repeats, and each repeat is divided into 5 feathers. The experimental animals were kept in three cages with infrared lamps, and the individual groups were kept separately, fed ad libitum and were given water, and vaccinated with new bivalent multivalent vaccine on day 20. Experiments 1-21 d all used meat chicken granules (trade name: meat chicken granules, xuzhou Yisheng poultry Co., ltd.). Test 22-42 d, wherein a control group is fed with a chicken in meat batch (Xuzhou Yisheng poultry Co., ltd., trade name: chicken in meat batch), 0.95%, 1.90% and 3.80% of the feed additive of examples 1-3 are added into the chicken in meat batch (Xuzhou Yisheng poultry Co., ltd., trade name: chicken in meat batch) respectively, and after the feed additive is mixed uniformly, the feed is fed for daily health care and growth promotion of livestock, and the test period is 21 d.
Analysis of variance was performed on each data using SPSS22.0 statistical software and the results were expressed as mean ± standard error (or standard deviation).
The broiler is fed for 42 days, the weight of all the tested chickens is weighed by taking the repetition as a unit, and the feed consumption (kg), the average feed intake (g), the weight gain (kg), the average daily weight gain (g) and the feed consumption weight gain ratio (g/g) are calculated. 3-5 data of 3 different individuals are measured for each group, and each index is averaged for analysis of variance to be used as measurement data.
Average feed intake (g) =feed consumption/day
Weight gain (kg) =end weight-start weight
Average daily gain (g) =weight gain/day
Feed consumption weight gain ratio (g/g) =feed amount/weight gain
Table 2 Compound Chinese herbal medicine feed for daily health care and growth promotion of livestockInfluence of additives on the productivity of broiler chickens
| Group of | Feed consumption/kg | Average feed intake/g | Weight gain/kg | Average daily gain/g | Ratio of material to weight |
| Control group | 4.70±0.01 | 111.86±0.31 | 2.72±0.07 B | 64.82±1.55 B | 1.73±0.04 A |
| Example 1 0.95% group | 4.70±0.02 | 111.95±0.39 | 2.81±0.06 B | 66.81±1.33 B | 1.68±0.03 A |
| Example 1.90% group | 4.72±0.01 | 112.43±0.18 | 2.88±0.14 A | 68.62±3.32 A | 1.64±0.08 B |
| Example 1.80% group | 4.73±0.04 | 112.57±1.00 | 2.93±0.14 A | 69.85±3.24 A | 1.61±0.06 B |
| Example 2 0.95% group | 4.65±0.07 | 110.81±1.64 | 2.76±0.06 B | 65.72±1.37 B | 1.69±0.04 A |
| Example 2.90% group | 4.71±0.01 | 112.14±0.34 | 2.80±0.11 A | 66.70±2.61 A | 1.68±0.06 B |
| Example 2.80% group | 4.72±0.05 | 112.33±1.21 | 2.90±0.11 A | 68.95±2.54 A | 1.63±0.06 B |
| Example 3 0.95% group | 4.64±0.06 | 110.38±1.54 | 2.76±0.06 B | 65.74±1.40 B | 1.68±0.05 A |
| Example 3 1.90% group | 4.69±0.03 | 111.67±0.75 | 2.81±0.10 A | 66.88±2.48 A | 1.67±0.06 B |
| Example 3.80% group | 4.72±0.04 | 112.38±1.06 | 2.91±0.13 A | 69.40±3.11 A | 1.62±0.07 B |
Note that: the same column of data shoulder marks different lowercase letters represent that the difference is significant (P < 0.05), different uppercase letters represent that the difference is significant (P < 0.01), no letters or the same letters represent that the difference is not significant (P > 0.05).
The whole course of the broiler feeding test has no morbidity and mortality. As can be seen from table 2, the feed consumption and average feed intake were higher for each test group compared to the control group, but there was no significant difference (P > 0.05); the weight gain and average daily weight gain of each test group are improved, and compared with a control group, the weight gain of a 1.90% group and a 4.19% group of compound Chinese herbal medicine feed additive group for daily health care and growth promotion of livestock is increased by 5.88%, 7.72% (P < 0.01), and the material consumption weight gain ratio is reduced by 5.20%, 6.93% (P < 0.01); the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock is shown to have a certain promotion effect on the growth performance of broiler chickens.
TABLE 3 influence of Compound Chinese herbal medicine feed additive for daily health promotion and growth promotion of livestock on duodenal structure of broiler chickens
Note that: the same column of data shoulder marks different lowercase letters represent that the difference is significant (P < 0.05), different uppercase letters represent that the difference is significant (P < 0.01), no letters or the same letters represent that the difference is not significant (P > 0.05).
As can be seen from table 3, the duodenal wall thickness was significantly increased (P < 0.05) for each of the test group chickens compared to the control group; the fluff height is extremely increased (P < 0.01); the crypt depth was reduced, 0.95% of the group crypt depths were very significantly reduced (P < 0.01).
2. Influence on immune function of broiler chickens
Reagents and instrumentation: superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit (lot 20191016) was purchased from beijiegui biotechnology ltd. Test instrument: multiskan Sky full wave microplate reader (Simer Feishier technologies (China) Inc., 51119570), fully automatic tissue dehydrator, fully automatic rotary microtome, research grade fluorescence microscopy imaging system (Lycra, germany).
The implementation process comprises the following steps: 10 broiler chickens were randomly selected from each group of trial 42d, collected from the subpterygoid veins, taken from the spleen, bilateral thymus and bursa, stripped of fat and connective tissue, weighed and the immune organ index (g/kg) calculated. Collecting blood from the pterygoid vein, standing for 30 min, and centrifuging at 2000 r/min for 15 min to obtain serum.
Organ index (g/kg) =visceral organ weight/living body weight.
TABLE 4 Effect of Compound Chinese herbal medicine feed additive for daily health promotion and growth promotion of livestock on immune organ index of broiler chickens
Note that: the same column of data shoulder marks different lowercase letters represent that the difference is significant (P < 0.05), different uppercase letters represent that the difference is significant (P < 0.01), no letters or the same letters represent that the difference is not significant (P > 0.05).
The individual groups of broilers were fed to 42d, spleen, bilateral thymus, bursa of Fabricius were weighed and the immune organ index (g/kg) calculated, and the results are shown in Table 4, and the spleen index and thymus index of each test group were improved but not significantly different (P > 0.05) compared to the control group. The bursa fabricius index of each test group is improved, wherein 0.95% and 3.80% of the bursa fabricius index are obviously higher than that of the control group (P is less than 0.05), and the bursa fabricius index is respectively improved by 52.68% and 48.21%.
The influence of the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock on the bursa of Fabricius tissue structure of broiler chickens is shown in table 5, and the area of bursa Fabricius lymph follicles of each test group is larger than that of a control group and is remarkably increased (P is less than 0.01).
Table 5 effect of compound chinese herbal medicine feed additive for daily health care and growth promotion of livestock on bursa of Fabricius tissue structure of broiler chickens
Note that: the same column of data shoulder marks different lowercase letters represent that the difference is significant (P < 0.05), different uppercase letters represent that the difference is significant (P < 0.01), no letters or the same letters represent that the difference is not significant (P > 0.05).
As shown in table 6, the spleen nodule area of each test group chicken was significantly increased (P < 0.01) compared to the control group; periarterial lymphatic sheaths were all significantly increased (P < 0.05), with 1.90% and 3.80% being very significantly increased (P < 0.01), and 0.95% being significantly increased (P < 0.05).
Table 6 influence of compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock on spleen tissue structure of broiler chickens
Note that: the same column of data shoulder marks different lowercase letters represent that the difference is significant (P < 0.05), different uppercase letters represent that the difference is significant (P < 0.01), no letters or the same letters represent that the difference is not significant (P > 0.05).
3. Influence on the antioxidant capacity of broiler chickens
And (3) analyzing antioxidant indexes of the broiler serum, and measuring absorbance at a position of 450 nm by adopting a WST-1 method to calculate SOD activity. The MDA assay uses TBA to measure absorbance at 532 nm.
Table 7 effect of compound chinese herbal medicine feed additive for daily health care and growth promotion of livestock on SOD and MDA of broiler serum
Note that: the same column of data shoulder marks different lowercase letters represent that the difference is significant (P < 0.05), different uppercase letters represent that the difference is significant (P < 0.01), no letters or the same letters represent that the difference is not significant (P > 0.05).
As can be seen from table 7, the SOD content of each test group was higher than that of the control group, and the SOD content of the 3.80% dose group was significantly improved (P < 0.05); although there was no significant difference in MDA, the levels were lower than in the control group.
The embodiment shows that the traditional Chinese medicine residue compound extract granule can effectively improve the feed utilization rate, promote the growth of broiler chickens, promote the development of immune organs, enhance the immune function and improve the oxidation capability. And compared with the embodiment 2 and the embodiment 3, the embodiment 1 has the best effect and achieves good technical effect.
The compound Chinese herbal medicine feed additive which is used for daily health care and growth promotion of livestock is added into daily ration, so that the development of immune organs such as spleen, thymus, bursa of Fabricius and the like can be promoted; promoting lymphocyte proliferation; promoting the microbial environment balance of intestinal tracts; enhancement of the small intestine's ability to absorb nutrients; the capability of resisting bacteria and viruses of organisms is enhanced, and experimental basis is provided for the Chinese herbal medicines as immunopotentiators to be added into feeds.
Example 7 Effect on pig growth
A subject: the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock prepared in the example 1. The effect of the feed additive of this example was examined by feeding test as follows.
The single factor design is adopted, 100 parts (male and female halves) of the external ternary growing-finishing pigs with health conditions and close weight are selected, and the external ternary growing-finishing pigs are randomly divided into 4 treatment groups (each treatment group comprises 5 repeated groups, and 5 pigs in each repeated group) which are respectively a control group and 3 test groups (0.95% group, 1.90% group and 3.80% group). The 4 treatment groups were fed with basal diet (see us NRC (2012) pig nutritional needs). Wherein, 3 different contents of the feed additives prepared in the example 1 are added into the basic ration adopted by the 3 test groups (the addition proportion of the feed additives is 0.95%, 1.90% and 3.80% of the basic ration). Feeding tests were performed according to the above requirements, pre-feeding period 7d, test period 60d. The feeding management program and the immunization program in the test period are carried out by referring to a conventional commercial pig feeding management manual, the consumption of materials in the test period is recorded, the materials are weighed, the materials are fed for 60 days, and after the test is finished, all indexes of the production performance of the pigs are measured. The analysis of variance was performed on each data using SPSS22.0 statistical software, and the results were expressed as mean.+ -. Standard error (or standard deviation), and each index of the productivity of pigs was shown in Table 8.
TABLE 8 pig production performance index
| Group of | Initial weight (kg) | Last weight (kg) | Weight gain (kg) | Average daily gain (kg) | Ratio of weight (kg/kg) |
| Control group | 82.18±2.56 | 134.46±3.43 | 52.28±5.67 B | 0.87±0.09 B | 3.58±0.39 A |
| 0.95% group | 81.25±1.27 | 139.73±2.62 | 58.48±2.58 B | 0.97±0.04 B | 3.18±0.13 A |
| 1.90% group | 80.87±1.45 | 140.58±1.74 | 59.71±2.25 A | 1.00±0.04 A | 3.11±0.14 B |
| 3.80% group | 80.46±1.14 | 143.20±3.14 | 62.74±3.64 A | 1.05±0.06 A | 2.91±0.20 B |
Note that: the same column of data shoulder marks different lowercase letters represent that the difference is significant (P < 0.05), different uppercase letters represent that the difference is significant (P < 0.01), no letters or the same letters represent that the difference is not significant (P > 0.05).
As can be seen from Table 8, the feed consumption and average feed intake of each test group were higher than those of the control group, but there was no significant difference (P > 0.05); the weight gain and average daily weight gain of each test group are improved, and compared with a control group, the weight gain of a 1.90% group and a 3.80% group of compound Chinese herbal medicine feed additive group for daily health care and growth promotion of livestock is increased by 14.21% and 20.01% (P is less than 0.01), and the material consumption weight gain ratio is reduced by 13.13% and 18.72% (P is less than 0.01); the different proportion feed additives have different influences on pig production performance, wherein 3.80% of the group has the highest average weight gain, the highest growth speed, the lowest feed weight ratio and the highest feed conversion efficiency, and the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock has the effects of promoting pig growth and improving feed utilization rate.
While the foregoing description illustrates and describes the preferred embodiments of the present application, it is to be understood that this application is not limited to the disclosed versions thereof, but is not to be construed as an admission that the application is not entitled to antedate such additional embodiments, but is susceptible of various other combinations, modifications and environments, and is susceptible of modification within the scope of the application herein, by way of the foregoing teachings or by way of skill or knowledge in the relevant art. And that modifications and variations which do not depart from the spirit and scope of the present invention are intended to be within the scope of the appended claims.
Claims (4)
1. A compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock is characterized by being prepared from the following raw materials in parts by weight:
10-20 parts of isatis root enzymolysis polysaccharide, 1-2 parts of sophora alopecuroide alkaloid, 5-15 parts of salvia miltiorrhiza residue fermentation polysaccharide, 5-10 parts of shaddock peel polysaccharide and 5-15 parts of honeysuckle stem polysaccharide;
the preparation method of the compound Chinese herbal medicine feed additive comprises the following steps:
(1) Taking the isatis root crude polysaccharide, wherein the liquid medicine ratio is 1:5, adding ethanol with the volume concentration of 60%, cold soaking and extracting at 4 ℃ for 6 h, collecting precipitate, volatilizing the ethanol in the obtained precipitate, and freeze-drying to obtain the isatis root refined polysaccharide; dissolving radix Isatidis refined polysaccharide in water, adding cellulase for enzymolysis, performing enzymolysis at 40deg.C for 4 h at 200U/mL, heating with boiling water for 15 min, cooling, centrifuging, collecting supernatant, concentrating under reduced pressure to obtain soft extract, and vacuum drying to obtain radix Isatidis enzymolysis polysaccharide;
(2) Taking herba Sophorae Alopecuroidis,removing impurities and crushing, wherein the liquid medicine ratio is 1:5, adding ethanol with the volume concentration of 70% for ultrasonic extraction of 1.5 and h, filtering, and concentrating the filtrate under reduced pressure to obtain extract; dissolving the extract with appropriate amount of acid water to obtain acid water solution, adjusting pH to 10 with 50g/L sodium hydroxide solution, and adjusting pH to 5000 r min -1 Centrifuging, taking supernatant, and adding an equal volume of 1.0mol/L NaCl solution to obtain adsorption liquid; preparing macroporous adsorption resin, namely selecting DF01 resin, fully swelling the resin, filling the resin into a glass column, enabling the resin to pass through an adsorption liquid from top to bottom, eluting by using ethanol with the volume concentration of 70% as an eluent, wherein the elution adsorption flow rate is 4 BV/h; collecting effluent, concentrating under reduced pressure, and vacuum drying to obtain herba Sophorae Alopecuroidis alkaloid;
(3) Taking the water extraction residues of the red sage root according to the liquid medicine ratio of 1:8, recovering fat-soluble components by refluxing 3 h at 55 ℃ with 70% ethanol, drying, placing in a culture medium, fermenting with medicinal fungus, wherein the fermentation strain is Ganoderma lucidum G1, the inoculation amount is 10%, the temperature is 20 ℃, and the ratio of radix Salviae Miltiorrhizae to testa Tritici is 3:1, a step of; air drying at 60deg.C to obtain Saviae Miltiorrhizae radix residue fermented polysaccharide;
(4) Taking coarse powder of shaddock peel, and adding the following components in percentage by mass: 1.5 cellulase and pectase, the enzyme dosage is 3.0%, and disodium hydrogen phosphate citric acid buffer solution with pH value of 4.0 is added, the feed liquid ratio is 1:30, the extraction temperature is 40 ℃, and the extraction time is 60 min; shaking at constant temperature, filtering, collecting filtrate, evaporating, concentrating, and cooling; adding absolute ethyl alcohol for dilution, wherein the adding amount is 1.5 times of that of the concentrated solution, standing for 30 min, and performing suction filtration to obtain a filter cake, namely shaddock peel polysaccharide;
(5) Pulverizing caulis Lonicerae, sieving with 65 mesh sieve, adding distilled water, and mixing with liquid material at a ratio of 30:1, heating ultrasonic extraction for 30 min, wherein the ultrasonic power is 180W, and the extraction temperature is 60 ℃; suction filtering, rotary evaporating and concentrating the filtrate, precipitating with ethanol overnight, centrifuging the filtrate, dissolving the precipitate with ultrapure water, separating with ceramic membrane UF-50 with feed flow of 25 mL/min, ultrafiltration temperature of 50deg.C and operation pressure of 0.2 MPa; collecting the permeate, concentrating, and vacuum drying to obtain the caulis Lonicerae polysaccharide.
2. The compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock according to claim 1 is characterized by being prepared from the following raw materials in parts by weight:
10 parts of isatis root enzymolysis polysaccharide, 1 part of sophora alopecuroide alkaloid, 10 parts of salvia miltiorrhiza residue fermentation polysaccharide, 5 parts of shaddock peel polysaccharide and 5 parts of honeysuckle stem polysaccharide;
or:
15 parts of isatis root enzymolysis polysaccharide, 2 parts of sophora alopecuroide alkaloid, 15 parts of salvia miltiorrhiza residue fermentation polysaccharide, 10 parts of shaddock peel polysaccharide and 10 parts of honeysuckle stem polysaccharide;
or:
20 parts of isatis root enzymolysis polysaccharide, 1 part of sophora alopecuroide alkaloid, 15 parts of salvia miltiorrhiza residue fermentation polysaccharide, 10 parts of shaddock peel polysaccharide and 10 parts of honeysuckle stem polysaccharide.
3. The use of the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock as claimed in claim 1 or 2 for preparing an animal feed additive for promoting animal growth, enhancing immunity and improving antibacterial and antiviral abilities.
4. An animal feed for promoting growth and enhancing immunity, which is characterized by comprising the compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock according to claim 1 or 2.
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| CN102603914A (en) * | 2012-04-20 | 2012-07-25 | 河南中医学院 | Preparation method of Caulis Lonicerae polysaccharide |
| CN104068284A (en) * | 2014-07-16 | 2014-10-01 | 山东新希望六和集团有限公司 | Compound feed used for curing gosling plague and preparation method of compound feed |
| CN107594132A (en) * | 2017-10-30 | 2018-01-19 | 赣州播恩生物技术股份有限公司 | The feed addictive of pigling immunity can be improved |
| CN110115319A (en) * | 2018-11-04 | 2019-08-13 | 团风开源饲料科技有限公司 | A kind of raising immunity of organisms composite Chinese herbal pig feed additive and preparation method |
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2020
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102603914A (en) * | 2012-04-20 | 2012-07-25 | 河南中医学院 | Preparation method of Caulis Lonicerae polysaccharide |
| CN104068284A (en) * | 2014-07-16 | 2014-10-01 | 山东新希望六和集团有限公司 | Compound feed used for curing gosling plague and preparation method of compound feed |
| CN107594132A (en) * | 2017-10-30 | 2018-01-19 | 赣州播恩生物技术股份有限公司 | The feed addictive of pigling immunity can be improved |
| CN110115319A (en) * | 2018-11-04 | 2019-08-13 | 团风开源饲料科技有限公司 | A kind of raising immunity of organisms composite Chinese herbal pig feed additive and preparation method |
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