CN106913670B - Chinese medicinal biological preparation for promoting growth and enhancing immunity of poultry and preparation method thereof - Google Patents
Chinese medicinal biological preparation for promoting growth and enhancing immunity of poultry and preparation method thereof Download PDFInfo
- Publication number
- CN106913670B CN106913670B CN201710288598.2A CN201710288598A CN106913670B CN 106913670 B CN106913670 B CN 106913670B CN 201710288598 A CN201710288598 A CN 201710288598A CN 106913670 B CN106913670 B CN 106913670B
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- traditional chinese
- parts
- culture medium
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 67
- 244000144977 poultry Species 0.000 title claims abstract description 28
- 230000036039 immunity Effects 0.000 title claims abstract description 11
- 230000001737 promoting effect Effects 0.000 title claims abstract description 10
- 230000002708 enhancing effect Effects 0.000 title abstract description 7
- 239000003814 drug Substances 0.000 claims abstract description 106
- 238000000034 method Methods 0.000 claims abstract description 17
- 244000303040 Glycyrrhiza glabra Species 0.000 claims abstract description 13
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims abstract description 13
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims abstract description 13
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims abstract description 12
- 235000011477 liquorice Nutrition 0.000 claims abstract description 12
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract 4
- 239000001963 growth medium Substances 0.000 claims description 57
- 244000005700 microbiome Species 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 27
- 238000009630 liquid culture Methods 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 24
- 235000015097 nutrients Nutrition 0.000 claims description 22
- 238000012258 culturing Methods 0.000 claims description 20
- 230000001580 bacterial effect Effects 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000012153 distilled water Substances 0.000 claims description 13
- 241000245665 Taraxacum Species 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 241000411851 herbal medicine Species 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 9
- 239000003124 biologic agent Substances 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 7
- 241000589902 Leptospira Species 0.000 claims description 6
- 230000003213 activating effect Effects 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000006916 nutrient agar Substances 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 241001673966 Magnolia officinalis Species 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 238000005728 strengthening Methods 0.000 claims description 2
- 241000271566 Aves Species 0.000 claims 2
- 229940079593 drug Drugs 0.000 claims 1
- 238000000855 fermentation Methods 0.000 abstract description 16
- 230000004151 fermentation Effects 0.000 abstract description 16
- 230000000813 microbial effect Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 9
- 238000000605 extraction Methods 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 206010059866 Drug resistance Diseases 0.000 abstract description 4
- 231100000331 toxic Toxicity 0.000 abstract description 4
- 230000002588 toxic effect Effects 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 230000006978 adaptation Effects 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 230000007721 medicinal effect Effects 0.000 abstract description 2
- 240000001949 Taraxacum officinale Species 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 30
- 239000000243 solution Substances 0.000 description 26
- 241000287828 Gallus gallus Species 0.000 description 24
- 230000002354 daily effect Effects 0.000 description 19
- 235000013594 poultry meat Nutrition 0.000 description 19
- 235000021050 feed intake Nutrition 0.000 description 14
- 210000000952 spleen Anatomy 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 9
- 241001570521 Lonicera periclymenum Species 0.000 description 8
- 235000013330 chicken meat Nutrition 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 210000004072 lung Anatomy 0.000 description 6
- 210000000056 organ Anatomy 0.000 description 6
- 210000002784 stomach Anatomy 0.000 description 6
- 206010011224 Cough Diseases 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 206010062717 Increased upper airway secretion Diseases 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 5
- 208000026435 phlegm Diseases 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 239000005017 polysaccharide Substances 0.000 description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 210000001669 bursa of fabricius Anatomy 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 210000002421 cell wall Anatomy 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002212 flavone derivatives Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 229930003935 flavonoid Natural products 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 235000017173 flavonoids Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 210000002216 heart Anatomy 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 206010000060 Abdominal distension Diseases 0.000 description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 2
- 241000555712 Forsythia Species 0.000 description 2
- 240000008917 Glycyrrhiza uralensis Species 0.000 description 2
- 235000000554 Glycyrrhiza uralensis Nutrition 0.000 description 2
- 102000018997 Growth Hormone Human genes 0.000 description 2
- 108010051696 Growth Hormone Proteins 0.000 description 2
- 244000167230 Lonicera japonica Species 0.000 description 2
- 235000017617 Lonicera japonica Nutrition 0.000 description 2
- 206010068319 Oropharyngeal pain Diseases 0.000 description 2
- 201000007100 Pharyngitis Diseases 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 229940079919 digestives enzyme preparation Drugs 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 235000008216 herbs Nutrition 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 2
- 235000005493 rutin Nutrition 0.000 description 2
- 229960004555 rutoside Drugs 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 229940126680 traditional chinese medicines Drugs 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- OFQCQIGMURIECL-UHFFFAOYSA-N 2-[2-(diethylamino)ethyl]-2',6'-dimethylspiro[isoquinoline-4,4'-oxane]-1,3-dione;phosphoric acid Chemical compound OP(O)(O)=O.O=C1N(CCN(CC)CC)C(=O)C2=CC=CC=C2C21CC(C)OC(C)C2 OFQCQIGMURIECL-UHFFFAOYSA-N 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 206010006298 Breast pain Diseases 0.000 description 1
- 206010006312 Breast swelling Diseases 0.000 description 1
- 208000027312 Bursal disease Diseases 0.000 description 1
- 241000208828 Caprifoliaceae Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 208000015220 Febrile disease Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241000218378 Magnolia Species 0.000 description 1
- 241000218377 Magnoliaceae Species 0.000 description 1
- 208000006662 Mastodynia Diseases 0.000 description 1
- 241000207834 Oleaceae Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 206010070863 Toxicity to various agents Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000021316 daily nutritional intake Nutrition 0.000 description 1
- 230000025507 digestive system development Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000006196 drop Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 201000003453 lung abscess Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 201000009240 nasopharyngitis Diseases 0.000 description 1
- 230000024121 nodulation Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000013613 poultry product Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 229940126673 western medicines Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
- A61K36/575—Magnolia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a poultry traditional Chinese medicine biological preparation for promoting growth and enhancing immunity and a preparation method thereof. Compared with the prior art, the poultry Chinese medicinal biological preparation contains dandelion, honeysuckle, fructus forsythiae, dried orange peel, mangnolia officinalis and liquorice, and the extraction rate of the effective components of the medicaments is increased after microbial fermentation, so that the poultry Chinese medicinal biological preparation is easier to absorb and has more sufficient medicinal effect; the invention is a pure Chinese medicinal preparation, has no toxic or side effect, and can not generate drug resistance after long-term use; the microbial fermentation method adopted by the invention has mild reaction conditions, can protect the effective components of the traditional Chinese medicine from being damaged to the maximum extent, and can increase the content of certain effective components and generate new medicinal components, thereby improving the curative effect of the medicine, enlarging the adaptation diseases and reducing the administration dosage.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine biological preparation for promoting growth and enhancing immunity of poultry and a preparation method thereof.
Background
With the rapid development of the chicken industry in large scale and intensification, various diseases often occur, and veterinary drugs are used increasingly frequently. Antibiotics are widely used in animal production as feed additives, but simultaneously bring about a plurality of problems, such as the generation of drug resistance, antibiotic residues in livestock and poultry products, and the negative effects of imbalance of normal flora in livestock and poultry bodies. Chicks are generally chickens below 7 weeks old and have the physiological characteristics of incomplete body temperature regulation mechanism, incomplete digestive system development, weak resistance, strong sensitivity and the like. The weak resistance makes the chicks easy to invade the body by exogenous pathogenic factors, thereby causing the diseases. Common bacterial diseases such as colibacillosis, pullorum disease and the like seriously affect the growth and development of chicks and bring great economic loss to the poultry industry. In order to meet the requirements of consumers, the traditional Chinese medicine chicken raising technology is produced. The traditional Chinese medicine not only has the effects of preventing and treating diseases, improving immunity, promoting growth and development and the like, but also has small toxic and side effects compared with western medicines, is not easy to generate drug resistance, has no residue and is low in cost. Relevant researches show that Chinese herbal medicine resources are reasonably developed and utilized, and the traditional Chinese medicines are used for gradually replacing conventional antibiotics, somatotropin and chemical medicine additives, so that the Chinese herbal medicine composition has a wide prospect for reducing veterinary medicine residues, developing the antibiotics, somatotropin and chemical medicine additives of the green chicken raising industry, reducing the veterinary medicine residues and developing the green chicken raising industry. The use of Chinese herbal medicines, enzyme preparations and probiotics in animal production is predicted to be an inevitable way to replace antibiotics. If a product integrating Chinese herbal medicines, enzyme preparations and probiotics is prepared by fermenting the Chinese herbal medicines by utilizing some beneficial microorganisms, the development of animal husbandry can be more powerfully promoted.
The Chinese medicine has a long fermentation history, as early as more than four thousand years, Chinese people have learned to use fermentation technology to brew wine and then are used for producing foods such as sauce, vinegar, fermented soya beans and the like in sequence. Modern biotechnology started to be applied to fermentation in the 80 th 20 th century, and the process and mechanism of fermentation processing were explored from the perspective of modern science, which is a typical biological conversion process. The modern Chinese medicine fermentation technology, also called Chinese medicine biotransformation technology, is a new Chinese medicine modern pharmacy technology formed by combining modern micro-ecology, bioengineering, fermentation engineering technology and other subjects on the basis of inheriting the traditional Chinese medicine fermentation processing method. The cell of the plant Chinese herbal medicine has cell walls, the main components of the cell walls comprise fibers, proteins and the like, the cell walls are difficult to damage by some conventional extraction methods, and active ingredients in the cell are difficult to dissolve out, so that the effective ingredients of the Chinese herbal medicine are not fully extracted, and the extraction rate is low. The microbial fermentation can produce various enzyme substances, such as cellulase, amylase, protease and the like, and can destroy plant cell walls, so that active ingredients in cells are separated out in a large amount, and the extraction rate of effective ingredients of Chinese herbal medicines is improved.
Disclosure of Invention
In order to solve the technical problems, the invention provides the traditional Chinese medicine biological preparation for promoting growth and strengthening immunity and the preparation method thereof, which aim to solve the problems that the extraction rate of the effective components of the Chinese herbal medicine is improved by microbial fermentation, the growth and development of livestock and poultry are promoted, the immunity is enhanced, and the organic combination of the traditional Chinese medicine formula and the modern biotechnology is realized.
The technical scheme adopted by the invention is as follows: a Chinese medicinal biological preparation for promoting growth and enhancing immunity of fowl is prepared by fermenting Chinese medicinal composition with microorganism;
the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 2-6 parts of dandelion, 2-5 parts of honeysuckle, 2-5 parts of fructus forsythiae, 1-5 parts of dried orange peel, 1-5 parts of cortex magnoliae officinalis and 1-5 parts of liquorice.
Preferably, the raw materials comprise the following components in parts by weight: 4 parts of dandelion, 3 parts of honeysuckle, 3 parts of fructus forsythiae, 2 parts of dried orange peel, 2 parts of magnolia officinalis and 3 parts of liquorice.
Preferably, the microorganism is bacillus subtilis.
A preparation method of a traditional Chinese medicine biological preparation for promoting growth and enhancing immunity of poultry is characterized by comprising the following steps:
step one, preparing a traditional Chinese medicine culture medium: respectively screening, cleaning and drying dandelion, honeysuckle, fructus forsythiae, dried orange peel, cortex magnoliae officinalis and liquorice, and mixing to obtain a traditional Chinese medicine mixture, crushing the traditional Chinese medicine mixture into medicinal material fine powder, and adding distilled water into the medicinal material fine powder to obtain a traditional Chinese medicine culture medium;
step two, activating microorganisms: placing the microorganism into a liquid culture medium for inoculation and culture, and then carrying out passage twice to obtain the activated microorganism;
step three, preparing a seed solution: inoculating the activated microorganism obtained in the step two into a liquid culture medium, and performing shake culture to obtain the seed liquid;
step four, preparing the traditional Chinese medicine biological preparation: adjusting the pH value of the traditional Chinese medicine culture medium obtained in the step one, sterilizing at high temperature, cooling to room temperature, inoculating the seed solution, and placing into a shaking table shaking incubator for shaking culture to obtain the traditional Chinese medicine biological preparation.
Preferably, the second step is specifically: inoculating microbial strain into nutrient broth liquid culture medium, culturing at 30-40 deg.C for 12-30 hr, and passaging twice; inoculating the bacterial liquid after two passages to a nutrient agar solid culture medium by using a leptospira, culturing for 12-30h at the temperature of 30-40 ℃, then selecting a single bacterial colony to a nutrient broth liquid culture medium, and culturing for 12-30h at the temperature of 30-40 ℃ to obtain the activated microorganism strain.
Preferably, the third step is specifically: inoculating 0.5-5% of the activated microorganism obtained in the step two into a nutrient broth liquid culture medium, and then placing the culture medium in a shaking incubator at the culture temperature of 30-40 ℃, the culture rotation speed of 100-200r/min and the culture time of 12-30h to obtain the seed liquid.
Preferably, the pH in step four is 4.0 to 7.0.
Preferably, the high-temperature sterilization conditions in the fourth step are as follows: the temperature is 105 deg.C, and the sterilization time is 20 min.
Preferably, the inoculation amount of the seed liquid in the fourth step is 0.5-8% of the weight of the traditional Chinese medicine culture medium.
Preferably, the shake culture process conditions in the fourth step are as follows: the temperature is 25-45 ℃, the rotating speed is 120-240 r/min, and the culture time is 12-84 h.
Has the advantages that: compared with the prior art, the poultry Chinese medicinal biological preparation for promoting growth and enhancing immunity and the preparation method thereof provided by the invention have the advantages that the raw materials comprise dandelion, honeysuckle, fructus forsythiae, dried orange peel, mangnolia officinalis and liquorice, the extraction rate of the effective ingredients of the medicaments is increased after microbial fermentation, the medicaments are easier to absorb, and the medicinal effect is more sufficient; the invention is a pure Chinese medicinal preparation, has wide raw material sources, low cost and no toxic or side effect, and can not generate drug resistance after long-term use; the traditional Chinese medicine preparation has poor palatability, effective components are not easy to dissociate from plant cells after oral administration and are fully absorbed by organisms, the bioavailability is low, and the decoction has great destructiveness on the effective components of the traditional Chinese medicine in the preparation process, so that the due curative effect of the medicine is reduced; the microbial fermentation method adopted by the invention has mild reaction conditions, can protect the effective components of the traditional Chinese medicine from being damaged to the maximum extent, and can increase the content of certain effective components and generate new medicinal components, thereby improving the curative effect of the medicine, enlarging the adaptation diseases and reducing the administration dosage.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the present invention will be described in detail below with reference to the accompanying tables and specific embodiments.
Example 1A biological preparation of Chinese traditional medicine for poultry
(1) Selecting the raw materials of the poultry Chinese medicinal biological preparation:
dandelion, alias herba violae, herba lysimachiae, HUALANG, etc., and belongs to perennial herb of Compositae. Bitter, sweet and cold in flavor. It enters liver and stomach meridians. The efficacy is as follows: clearing away heat and toxic material, dispersing swelling and dissipating stagnation, inducing diuresis and treating stranguria. The application comprises the following steps: sores and abscess, lung abscess, conjunctival congestion, pharyngalgia, jaundice due to damp-heat pathogen, and heat stranguria.
Lonicera japonica Thunb, Lonicera japonica Thunb of Caprifoliaceae. Sweet and cold in flavor. It enters lung, heart and stomach meridians. The efficacy is as follows: clear heat and remove toxicity, disperse wind-heat. The application comprises the following steps: the attack of damp-heat, wind-heat type common cold, cough due to lung heat, sore throat, heat-toxin bloody dysentery, breast swelling and pain, carbuncle, swelling and sore.
Forsythia suspense, deciduous shrubs, commonly known as a string of gold in hong Kong, is a plant of the genus Forsythia of the family Oleaceae. Bitter and slightly cold in taste. It enters lung, heart and small intestine meridians. The efficacy is as follows: clear heat and remove toxicity, resolve swelling and dissipate nodulation. The application comprises the following steps: fever due to epidemic febrile disease, sore, yellow and swollen poison.
Pericarpium Citri Tangerinae, also known as pericarpium Citri Tangerinae, is mature pericarp of Rutaceae plant fructus Citri Tangerinae and its cultivar. Bitter, pungent and warm in flavor. It enters lung and spleen meridians. The efficacy is as follows: regulate qi to invigorate spleen, dry dampness and resolve phlegm. The application comprises the following steps: appetite decrease, abdominal pain, abdominal distension, diarrhea, phlegm-dampness cough.
Magnolia officinalis, also known as Magnolia officinalis, etc., is a plant of Magnoliaceae, or Magnolia. Bitter, pungent and warm in flavor. It enters spleen, stomach, lung and large intestine meridians. The efficacy is as follows: descend qi and relieve distension, dry dampness and resolve phlegm. The application comprises the following steps: indigestion, food retention and qi stagnation, abdominal distension and constipation, phlegm retention and cough and asthma.
Radix Glycyrrhizae, also known as "Gulao", Glycyrrhiza uralensis, Glycyrrhiza glabra, Glycyrrhiza uralensis, Leguminosae, perennial herbs. Sweet and mild in taste. It enters heart, lung, spleen and stomach meridians. The efficacy is as follows: invigorating spleen and replenishing qi, eliminating phlegm and relieving cough, regulating the middle warmer and relieving urgency, removing toxic substance, harmonizing the recipe, and relieving drug toxicity and toxicity. The application comprises the following steps: weakness of the spleen and stomach, lassitude, weakness, cough and dyspnea, sore throat, poisoning, sores and ulcers.
(2) The preparation proportion of the poultry Chinese medicinal biological preparation is as follows:
the raw materials in the biological preparation of the traditional Chinese medicine for the poultry are respectively mixed according to the mass parts shown in the table 1 to obtain the biological preparations I to III of the traditional Chinese medicine for the poultry with different mixing ratios.
TABLE 1 biological preparation of poultry Chinese traditional medicine with different mixing ratio (by weight)
Example 2: preparation method of traditional Chinese medicine biological preparation for poultry
Step one, preparing a traditional Chinese medicine culture medium: respectively screening, cleaning and drying 2 parts of dandelion, 2 parts of honeysuckle, 2 parts of fructus forsythiae, 1 part of dried orange peel, 1 part of cortex magnoliae officinalis and 1 part of liquorice, and mixing to obtain a traditional Chinese medicine mixture, crushing the traditional Chinese medicine mixture into 30-mesh medicinal material fine powder, and adding distilled water which is 10 times of the medicinal material fine powder in weight into the medicinal material fine powder to obtain a traditional Chinese medicine culture medium;
step two, activating microorganisms: inoculating microbial strain into nutrient broth liquid culture medium, culturing at 30-40 deg.C for 12 hr, and passaging twice; inoculating the bacterial liquid after two passages to a nutrient agar solid culture medium by using a leptospira, culturing for 12h at the temperature of 30 ℃, then selecting a single bacterial colony to a nutrient broth liquid culture medium, and culturing for 12h at the temperature of 30 ℃ to obtain the activated microorganism.
Step three, preparing a seed solution: inoculating activated microorganisms into a nutrient broth liquid culture medium, wherein the inoculation amount of the activated microorganisms is 0.5 percent of the weight of the nutrient broth liquid culture medium, and then placing the activated microorganisms in a shaking incubator for 12 hours at the culture temperature of 30 ℃, the culture rotation speed of 100r/min and the culture time of 12 hours to obtain seed liquid;
step four, preparing the traditional Chinese medicine biological preparation: adjusting the pH value of the traditional Chinese medicine culture medium obtained in the step one to 4.0, sterilizing at 105 ℃ for 20min, cooling to room temperature, inoculating a seed solution accounting for 0.5% of the weight of the traditional Chinese medicine culture medium, putting the seed solution into a shaking incubator, and culturing at 25 ℃ for 12h at a culture speed of 120r/min to obtain the traditional Chinese medicine biological preparation I.
Example 3: preparation method of traditional Chinese medicine biological preparation for poultry
Step one, preparing a traditional Chinese medicine culture medium: screening, cleaning and drying 6 parts of dandelion, 5 parts of honeysuckle, 5 parts of fructus forsythiae, 5 parts of dried orange peel, 5 parts of cortex magnoliae officinalis and 5 parts of liquorice respectively, and mixing to obtain a traditional Chinese medicine mixture, crushing the traditional Chinese medicine mixture into 80-mesh medicinal material fine powder, and adding distilled water which is 5 times of the medicinal material fine powder in weight into the medicinal material fine powder to obtain a traditional Chinese medicine culture medium;
step two, activating microorganisms: inoculating microbial strains into a nutrient broth liquid culture medium, culturing at 40 ℃ for 30h, and carrying out passage twice; inoculating the bacterial liquid after two passages to a nutrient agar solid culture medium by using a leptospira, culturing for 30h at the temperature of 40 ℃, then selecting a single bacterial colony to a nutrient broth liquid culture medium, and culturing for 30h at the temperature of 40 ℃ to obtain an activated microorganism seed;
step three, preparing a seed solution: inoculating activated microorganisms into a nutrient broth liquid culture medium, wherein the inoculation amount of the activated microorganisms is equal to 5% of the weight of the nutrient broth liquid culture medium, and then placing the activated microorganisms in a shaking incubator at the culture temperature of 40 ℃, the culture rotation speed of 200r/min and the culture time of 30h to obtain a seed solution;
step four, preparing the traditional Chinese medicine biological preparation: adjusting the pH value of the traditional Chinese medicine culture medium obtained in the step one to 7.0, sterilizing at 105 ℃ for 20min, cooling to room temperature, inoculating a seed solution accounting for 8% of the weight of the traditional Chinese medicine culture medium, putting the seed solution into a shaking table shaking incubator, and culturing at 45 ℃ for 84h at a rotation speed of 240r/min to obtain the traditional Chinese medicine biological preparation II.
Example 4: preparation method of traditional Chinese medicine biological preparation for poultry
Step one, preparing a traditional Chinese medicine culture medium: respectively screening, cleaning and drying 4 parts of dandelion, 3 parts of honeysuckle, 3 parts of fructus forsythiae, 2 parts of dried orange peel, 2 parts of cortex magnoliae officinalis and 3 parts of liquorice, and mixing to obtain a traditional Chinese medicine mixture, crushing the traditional Chinese medicine mixture into 40-mesh medicinal material fine powder, and adding distilled water which is 10 times of the medicinal material fine powder in weight into the medicinal material fine powder to obtain a traditional Chinese medicine culture medium;
step two, activating microorganisms: inoculating microbial strains into a nutrient broth liquid culture medium, culturing at 37 ℃ for 24h, and carrying out passage twice; inoculating the bacterial liquid after two passages to a nutrient agar solid culture medium by using a leptospira loop, culturing for 24h at the temperature of 37 ℃, then selecting a single bacterial colony to a nutrient broth liquid culture medium, and culturing for 24h at the temperature of 37 ℃ to obtain an activated microorganism seed;
step three, preparing a seed solution: inoculating activated microorganisms into a nutrient broth liquid culture medium, wherein the inoculation amount of the activated microorganisms is 1% of the weight of the nutrient broth liquid culture medium, and then placing the activated microorganisms in a shaking incubator at the culture temperature of 37 ℃, the culture rotation speed of 180r/min and the culture time of 24h to obtain a seed solution;
step four, preparing the traditional Chinese medicine biological preparation: adjusting the pH value of the traditional Chinese medicine culture medium obtained in the step one to 5.0, sterilizing at 105 ℃ for 20min, cooling to room temperature, inoculating a seed solution accounting for 2% of the weight of the traditional Chinese medicine culture medium, and then putting the seed solution into a shaking table shaking incubator, wherein the culture temperature is 35 ℃, the culture rotation speed is 210r/min, and the culture time is 24h, so as to obtain the traditional Chinese medicine biological preparation III.
Example 5: preparation method of traditional Chinese medicine biological preparation for poultry
Step one, preparing a traditional Chinese medicine culture medium: respectively screening, cleaning and drying 4 parts of dandelion, 3 parts of honeysuckle, 3 parts of fructus forsythiae, 2 parts of dried orange peel, 2 parts of cortex magnoliae officinalis and 3 parts of liquorice, and mixing to obtain a traditional Chinese medicine mixture, crushing the traditional Chinese medicine mixture into 40-mesh medicinal material fine powder, and adding distilled water which is 10 times of the medicinal material fine powder in weight into the medicinal material fine powder to obtain a traditional Chinese medicine culture medium;
step two, activating microorganisms: inoculating microbial strains into a nutrient broth liquid culture medium, culturing at 37 ℃ for 24h, and carrying out passage twice; inoculating the bacterial liquid after two passages to a nutrient agar solid culture medium by using a leptospira loop, culturing for 24h at the temperature of 37 ℃, then selecting a single bacterial colony to a nutrient broth liquid culture medium, and culturing for 24h at the temperature of 37 ℃ to obtain an activated microorganism seed;
step three, preparing a seed solution: inoculating activated microorganisms into a nutrient broth liquid culture medium, wherein the inoculation amount of the activated microorganisms is 1% of the weight of the nutrient broth liquid culture medium, and then placing the activated microorganisms in a shaking incubator at the culture temperature of 37 ℃, the culture rotation speed of 180r/min and the culture time of 24h to obtain a seed solution;
step four, preparing the traditional Chinese medicine biological preparation: adjusting the pH value of the traditional Chinese medicine culture medium obtained in the step one to 7.0, sterilizing at 105 ℃ for 20min, cooling to room temperature, inoculating a seed solution with the weight being 2% of that of the traditional Chinese medicine culture medium, and then putting the seed solution into a shaking table shaking incubator, wherein the culture temperature is 37 ℃, the culture rotation speed is 150r/min, and the culture time is 48h, so as to obtain the traditional Chinese medicine biological preparation IV.
Secondly, respectively carrying out drug effect determination on the poultry Chinese medicinal biological preparations I to IV prepared by the invention, taking the poultry Chinese medicinal biological preparation III as an example:
(1) determination of total flavone content
a. Preparation of control solutions
Taking 9.7mg of rutin control, precisely weighing, placing in a 50mL measuring flask, adding appropriate amount of methanol for dissolving, adding distilled water to scale, and shaking to obtain control solution (containing 0.194mg of rutin per 1m L).
b. Drawing of standard curve
Precisely measuring reference substance solutions 0.0 mL, 0.2 mL, 0.5 mL, 1.0mL, 1.5 mL, 2.0 mL, 2.5 mL and 3mL, respectively placing in 10mL volumetric flasks, adding distilled water to 3mL, adding 5% sodium nitrite solution 0.4mL, mixing, and standing for 6 min; then 0.4mL of 10 percent aluminum nitrate solution is added, shaken up and placed for 6 min; adding 4.0mL of 4% sodium hydroxide solution, adding distilled water to the scale, shaking, standing for 15min, and taking 0.0 as blank. And (4) measuring the absorbance at 510nm by using an ultraviolet spectrophotometer, and calculating to obtain a regression equation by taking the absorbance as a vertical coordinate and the mass concentration as a horizontal coordinate.
c. 3 samples were tested in parallel and the arithmetic mean was taken.
(2) Total polysaccharide assay
a. Preparation of glucose standard solution and phenol solution
Taking a glucose standard product dried to constant weight at 105 ℃, precisely weighing 10.0mg in a 100mL volumetric flask, adding distilled water to scale, and preparing into a glucose standard solution with the concentration of 0.1 mg/mL.
40g of phenol, 0.5g of zinc powder and NaHC 0320 mg were weighed out and distilled (air-condensed), and the fraction at 182 ℃ was collected. 5.0g of the fraction was weighed into a 100mL volumetric flask, and distilled water was added to the scale to prepare a 5% phenol solution.
b. Drawing of glucose standard curve
0.2 mL, 0.4mL, 0.6 mL, 0.8 mL, 1.0mL, 1.2 mL, 1.4 mL, 1.6mL of glucose standard solution were respectively pipetted into a test tube, water was added to 1.6mL, then 6% phenol 1.0mL and concentrated sulfuric acid 5.0mL were added, shaken well, 2mL of distilled water was used as a reference, the absorbance value was measured at 490nm after standing at room temperature for 25min, a glucose standard curve was drawn, and a regression equation was calculated.
c. 3 samples were tested in parallel and the arithmetic mean was taken.
TABLE 2 measurement results of the drug effect of the biological preparation of Chinese herbs
| Group of | Total flavone mg/g | Total polysaccharide mg/g |
| Chinese medicine biological preparation III | 83.15 | 26.11 |
| Chinese medicine biological preparation IV | 69.03 | - |
| Unfermented traditional Chinese medicine | - | 20.76 |
As can be seen from the above table, the content of total flavonoids in the biological traditional Chinese medicine preparation III is increased by 20.46% compared with the content of total flavonoids in the biological traditional Chinese medicine preparation IV, and the extraction of the total flavonoids as the active ingredients of the biological traditional Chinese medicine preparation III obtained under the fermentation conditions of the embodiment 4 is more sufficient than that of the biological traditional Chinese medicine preparation IV obtained under the fermentation conditions of the embodiment 5; the polysaccharide content of the traditional Chinese medicine biological preparation III measured by adopting a phenol-sulfuric acid method is increased by 25.77 percent compared with the polysaccharide content of the traditional Chinese medicine which is not fermented, the polysaccharide content of the traditional Chinese medicine composition is obviously increased by the fermentation treatment, and the total flavone of the effective components of the traditional Chinese medicine composition is more fully extracted.
(3) Clinical efficacy study
a. Study subjects: 180 healthy red feather broilers of 1 day old are taken and randomly divided into 6 groups, namely a blank control group (blank group), a bacterial liquid control group (bacterial liquid group), a traditional Chinese medicine biological agent test group (namely, non-sterilized group) I, II and III and a traditional Chinese medicine biological agent sterilization test group (IV), wherein each group is provided with 3 repetitions, and each repetition is 10. Cage culture is adopted, a trough and a water fountain are sufficient, food and water are taken freely, 24 hours of illumination are realized, the whole test time is 46 days, and the test time is divided into 2 stages (0-21 days old and 21-46 days old). Inoculating new-fluid dual vaccine at 8 days old, inoculating infectious bursal disease live vaccine at 21 days old, and inoculating by eye drop and nose drop.
The treatment of each group is as follows: the blank group is fed by adding 0.5% of distilled water into the word material, the bacteria liquid group is fed by adding 0.5% of bacteria liquid into the word material, the fermented traditional Chinese medicine sterilization group is fed by adding 0.5% of sterilized traditional Chinese medicine biological agent III into the word material, and the traditional Chinese medicine biological agent test groups I, II and III are respectively fed by adding 0.25%, 0.5% and 1.0% of traditional Chinese medicine biological agent II into the word material.
b. And (3) measuring growth performance indexes: and recording the feed amount, the residual feed amount and the scattered feed amount every day, and calculating the actual feed intake every day. The weight was measured on empty stomach in the morning of 1 day, 21 days and 46 days, respectively.
And calculating the average daily feed intake, average daily gain and material-weight ratio of each stage.
Actual feed intake is the amount of feed fed, the amount of residual feed and the amount of feed scattered
Average daily food intake is total actual food intake/test days
Average daily gain (end stage weight-initial stage weight)/days in stage
Material-to-weight ratio (actual total feed intake/total weight gain in stage)
TABLE 3 influence of Chinese medicinal biological preparation on growth performance index of broiler chicken
Note: the difference of different lower case letters is significant (0.01 < P < 0.05), and the difference of different upper case letters is significant (P < 0.01)
The results in the table show that the differences of the total feed intake, the average daily feed intake and the average daily gain of the broilers in each test group at the age of 0-21 days are extremely obvious (P is less than 0.01), the feed-weight ratios of the test groups are all lower than those of the blank group, and the differences are extremely obvious (P is less than 0.01); the average daily gain and feed weight of the broiler chickens in the test group II are extremely different from those of other test groups (P is less than 0.01), the average daily gain is increased by 27.3 percent compared with a blank group, and the feed weight ratio is reduced by 12.1 percent compared with the blank group. Compared with the bacterial liquid group, the total feed intake and the average daily feed intake of the test group II and the test group III have very obvious difference (P is less than 0.01). The average daily gain and the material-weight ratio of the test group II are very obvious in difference (P is less than 0.01) compared with the bacteria liquid group. In the age period of 21-46 days, the total feed intake and the average daily feed intake of the broilers of all groups except the group I are compared with those of the blank group, and the difference is very obvious (P is less than 0.01). Compared with the blank group, the average daily gain of the broilers in each test group is very obvious in difference (P is less than 0.01); compared with the blank group, the average daily gain of the broilers in the test I group is increased by 8.7 percent, the average daily gain of the broilers in the test II group is increased by 7.0 percent, the average daily gain of the broilers in the test III group is increased by 5.7 percent, and the average daily gain of the broilers in the test IV group is increased by 19.. The material weight ratio of each test group is obviously different (P is more than 0.01 and less than 0.05) compared with that of a blank group, wherein the material weight ratio of the test group II is minimum and is reduced by 18.2 percent compared with that of the blank group. Compared with the bacterial liquid group, the total feed intake, the average daily feed intake and the average daily gain of the groups I, II and III have very obvious difference (P is less than 0.01). The total feed intake, average daily feed intake, and average daily gain of the test groups I, II, and III were lower than those of the bacterial suspension group. Compared with the bacteria liquid group, the material weight ratio of the experiments II and III has obvious difference (P is more than 0.01 and less than 0.05), and the difference is lower than that of the bacteria liquid group.
c. Immune organ index: and (3) collecting blood of 46-day-old chickens, killing the chickens, weighing spleen and bursa of Fabricius, and calculating immune organ indexes.
Immune organ index (weight of immune organ (g)/weight of pre-slaughter fasting live (100g)
TABLE 4 influence of Chinese medicinal biological preparation on immune organ index of broiler chicken
| Group of | Spleen index | Bursal index |
| Group I | 0.157±0.032ab | 0.145±0.036 |
| Group II | 0.162±0.024a | 0.125±0.018 |
| Group III | 0.115±0.026b | 0.136±0.019 |
| Group IV | 0.166±0.031a | 0.157±0.058 |
| Bacterial liquid set | 0.127±0.028ab | 0.167±0.085 |
| Blank group | 0.117±0.051b | 0.129±0.018 |
Note: different lower case letters indicate significant differences (0.01 < P < 0.05)
The results in the table show that the spleen indexes of the broilers in the test group II and the test group IV are remarkably different (P is more than 0.01 and less than 0.05) compared with the blank group, and are not remarkably different (P is more than 0.05) compared with the bacteria liquid group; except group III, however, the spleen index of broilers in all test groups was higher than that of the blank group. The spleen indexes of the test groups I, II and IV are all higher than those of the bacteria liquid group. The bursa of Fabricius indexes of the broilers in each test group are not obvious in difference (P is more than 0.05) compared with those of a blank group and a bacteria liquid group, wherein the bursa of Fabricius indexes of the broilers in other test groups except the test II group are higher than those of the blank group, and the bursa of Fabricius indexes of the broilers in each test group are lower than those of the bacteria liquid group. The results are combined to obtain the traditional Chinese medicine biological preparation III which has certain promotion effect on the development of immune organs of the broiler chicken.
Finally, it should be noted that the above-mentioned description is only a preferred embodiment of the present invention, and those skilled in the art can make various similar representations without departing from the spirit and scope of the present invention.
Claims (7)
1. A preparation method of a traditional Chinese medicine biological preparation for promoting growth and strengthening immunity of poultry is characterized by comprising the following steps:
step one, preparing a traditional Chinese medicine culture medium: respectively screening, cleaning and drying dandelion, honeysuckle, fructus forsythiae, dried orange peel, mangnolia officinalis and liquorice, and then mixing to obtain a traditional Chinese medicine mixture, crushing the traditional Chinese medicine mixture into medicinal material fine powder, and adding distilled water into the medicinal material fine powder to obtain a traditional Chinese medicine culture medium; the raw material medicines comprise, by mass, 2-6 parts of dandelion, 2-5 parts of honeysuckle, 2-5 parts of fructus forsythiae, 1-5 parts of dried orange peel, 1-5 parts of mangnolia officinalis and 1-5 parts of liquorice;
step two, activating microorganisms: inoculating Bacillus subtilis strain into nutrient broth liquid culture medium, culturing at 30-40 deg.C for 12-30 hr, and passaging twice; inoculating the bacterial liquid after two passages to a nutrient agar solid culture medium by using a leptospira, culturing for 12-30h at the temperature of 30-40 ℃, then selecting a single bacterial colony to a nutrient broth liquid culture medium, and culturing for 12-30h at the temperature of 30-40 ℃ to obtain an activated microorganism seed;
step three, preparing a seed solution: inoculating the activated microorganism obtained in the step two into a liquid culture medium, and performing shake culture to obtain the seed liquid;
step four, preparing the traditional Chinese medicine biological preparation: adjusting the pH value of the traditional Chinese medicine culture medium obtained in the step one, sterilizing at high temperature, cooling to room temperature, inoculating the seed solution, and placing into a shaking table shaking incubator for shaking culture to obtain the traditional Chinese medicine biological preparation.
2. The method for preparing a growth-promoting and immunity-enhancing poultry traditional Chinese medicine biological preparation according to claim 1, which is characterized in that the raw materials comprise, by mass: 4 parts of dandelion, 3 parts of honeysuckle, 3 parts of fructus forsythiae, 2 parts of dried orange peel, 2 parts of magnolia officinalis and 3 parts of liquorice.
3. The method for preparing a growth-promoting and immunity-enhancing poultry Chinese medicinal biological agent according to claim 1, wherein the third step is specifically: inoculating 0.5-5% of the activated microorganism obtained in the step two into a nutrient broth liquid culture medium, and then placing the culture medium in a shaking incubator at the culture temperature of 30-40 ℃, the culture rotation speed of 100-200r/min and the culture time of 12-30h to obtain the seed liquid.
4. The method for preparing a growth-promoting and immunity-enhancing biological preparation of a Chinese herbal medicine for poultry according to claim 1 or 3, wherein the method comprises the following steps: the pH value in the fourth step is 4.0-7.0.
5. The method for preparing a growth-promoting and immunity-enhancing biological preparation of a Chinese herbal medicine for poultry according to claim 1 or 3, wherein the method comprises the following steps: the high-temperature sterilization conditions in the fourth step are as follows: the temperature is 105 deg.C, and the sterilization time is 20 min.
6. The method for preparing a growth-promoting and immunity-enhancing avian traditional Chinese medicine biological agent according to claim 4, wherein the method comprises the following steps: the inoculation amount of the seed liquid in the fourth step is 0.5 to 8 percent of the weight of the traditional Chinese medicine culture medium.
7. The method for preparing a growth-promoting and immunity-enhancing avian traditional Chinese medicine biological agent according to claim 5, wherein the method comprises the following steps: the shake culture process conditions in the fourth step are as follows: the temperature is 25-45 ℃, the rotating speed is 120-240 r/min, and the culture time is 12-84 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710288598.2A CN106913670B (en) | 2017-04-27 | 2017-04-27 | Chinese medicinal biological preparation for promoting growth and enhancing immunity of poultry and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710288598.2A CN106913670B (en) | 2017-04-27 | 2017-04-27 | Chinese medicinal biological preparation for promoting growth and enhancing immunity of poultry and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106913670A CN106913670A (en) | 2017-07-04 |
| CN106913670B true CN106913670B (en) | 2020-05-12 |
Family
ID=59568724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710288598.2A Active CN106913670B (en) | 2017-04-27 | 2017-04-27 | Chinese medicinal biological preparation for promoting growth and enhancing immunity of poultry and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106913670B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107625803A (en) * | 2017-10-19 | 2018-01-26 | 洛阳瑞华动物保健品有限公司 | A kind of Chinese medicine preparation preparation method and Traditional Chinese medicine extraction method for improving animal lactation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103504134A (en) * | 2012-06-15 | 2014-01-15 | 徐州金利饲料厂 | Meat duck feed additive |
| CN104922586A (en) * | 2015-01-19 | 2015-09-23 | 山东宝来利来生物工程股份有限公司 | Preparing and application of probiotics-fermented traditional Chinese medicinal preparation for preventing and treating coccidiosis in chicken |
-
2017
- 2017-04-27 CN CN201710288598.2A patent/CN106913670B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103504134A (en) * | 2012-06-15 | 2014-01-15 | 徐州金利饲料厂 | Meat duck feed additive |
| CN104922586A (en) * | 2015-01-19 | 2015-09-23 | 山东宝来利来生物工程股份有限公司 | Preparing and application of probiotics-fermented traditional Chinese medicinal preparation for preventing and treating coccidiosis in chicken |
Non-Patent Citations (1)
| Title |
|---|
| 中药乳膏剂对乳房炎奶牛免疫功能影响的研究;刘文等;《黑龙江畜牧兽医》;20081231(第05期);第89-91页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106913670A (en) | 2017-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103585433B (en) | Traditional Chinese medicine composition for treating oral ulcer | |
| CN112568324B (en) | Composite probiotics fermented traditional Chinese medicine feed additive and preparation method and application thereof | |
| CN114984114B (en) | Liquid fermentation traditional Chinese medicine microecological preparation and application thereof in aspect of resisting porcine epidemic diarrhea virus | |
| CN106165780A (en) | A kind of broiler Chinese medicine composite probiotics feed additive and preparation method thereof | |
| CN112156156B (en) | Veterinary traditional Chinese medicine antiviral oral liquid and preparation method thereof | |
| CN104208239B (en) | A kind of for preventing and treating the Chinese medicine composition of poultry bacillus coli, microbial ecological agent and preparation method | |
| CN114568602A (en) | Traditional Chinese medicine compound for improving growth performance and organism immunity of broiler chickens and preparation method and application thereof | |
| CN112426501B (en) | Traditional Chinese medicine microecological preparation for promoting growth and preventing diarrhea of calves and preparation method thereof | |
| CN112535239B (en) | Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof | |
| CN108887496A (en) | The preparation method of quick delactation's Chinese herbal medicine fermentation material after the wean of one broad sow | |
| CN106913670B (en) | Chinese medicinal biological preparation for promoting growth and enhancing immunity of poultry and preparation method thereof | |
| CN109717324A (en) | A kind of feed formula and its application for layer breeding | |
| CN110786414A (en) | Fermented traditional Chinese medicine additive for preventing African swine fever and preparation method thereof | |
| CN105943679A (en) | Traditional Chinese medicine probiotic compound preparation for poultry and preparation method thereof | |
| CN101664456A (en) | Chinese medicinal biological preparation of Shuanghuanglian oral liquid and preparation method thereof | |
| CN109646546B (en) | A Chinese medicinal composition for preventing and treating diarrhea of livestock, and its preparation method | |
| CN109432287B (en) | Traditional Chinese medicine oral liquid for preventing and treating excessive heat syndrome of poultry qi system and preparation method thereof | |
| CN102836212A (en) | Hepatoprotective composition | |
| CN111588801A (en) | Traditional Chinese medicine dispersible granule for preventing livestock and poultry epidemic diseases and preparation method thereof | |
| CN112544812A (en) | Mixed-strain fermentation type Chinese herbal medicine additive for laying hens in later period of laying, and preparation method and application thereof | |
| CN110200132A (en) | A kind of active flora herb fermenting object and preparation method thereof substituting feeding antibiotic | |
| CN110637934A (en) | Tea seed meal polysaccharide compound and application thereof in feed and treatment of porcine reproductive and respiratory syndrome | |
| CN116268196B (en) | Plant source composite additive and application thereof | |
| CN108531406A (en) | A kind of culture medium of Hericium erinaceus, lily biology conversion mycelium, lily biology convert mycelial extract and application thereof | |
| CN115006496B (en) | Chinese herbal medicine composition for treating pneumonia of foxes, raccoon dogs and martens |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220425 Address after: 402460 No. 2, Fifth Branch Road, Changzhou Street Park, Rongchang District, Chongqing Patentee after: CHONGQING XINONGDAKEXIN ANIMAL PHARMACEUTICAL Co.,Ltd. Address before: 400715 No. 1, natural road, Beibei District, Chongqing Patentee before: SOUTHWEST University |