CN101664456A - Chinese medicinal biological preparation of Shuanghuanglian oral liquid and preparation method thereof - Google Patents

Abstract

The invention relates to a Chinese medicinal biological preparation of Shuanghuanglian oral liquid and a preparation method thereof. The Chinese medicinal biological preparation of Shuanghuanglian oral liquid is prepared by combining biotechnology with conventional Chinese medicinal preparations, introducing EM original liquid into the Shuanghuanglian oral liquid for fermentation and metabolism and utilizing the biological metabolic transformation function and own nutritional value of the EM original liquid. The preparation method comprises: decocting and concentrating medicinal water of the Shuanghuanglian oral liquid to a concentration between 0.2 and 2 g/ml; inoculating the Shuanghuanglian oral liquid with the EM original liquid; fermenting the obtained product for 1 to 5 months at a temperature between 10 and 30 DEG C in light; and performing separation, extraction, refining and sterilization on obtained medicinal liquid and preparing the obtained product into various preparations.The Chinese medicinal biological preparation prepared by metabolizing the Shuanghuanglian oral liquid through the EM original liquid is enhanced in the aspects of enhancing immunity, resisting aging,treating model mice with kidney yin deficiency and the like, and is obviously superior to the conventional Shuanghuanglian oral liquid.

Description

Traditional Chinese biological preparation of SHUANGHUANGLIAN KOUFUYE and preparation method thereof

Technical field

The present invention relates to a kind of Chinese medicine preparation, particularly relate to traditional Chinese biological preparation of a kind of SHUANGHUANGLIAN KOUFUYE and preparation method thereof.

Background technology

Be planted in the Chinese medicine of traditional Chinese culture, developing history in several thousand has been arranged, and the history of Western medicine have only more than 100 year, in the modern medical service system, still Western medicine in the highest flight.Trace it to its cause, Western medicine is the monomer medicine, and molecular weight is little, is easy to enter human body cell, therefore, instant effect more direct to the treatment of focus; And Chinese medicine is compound recipe (colony) medicine, and molecular weight is big, is the mixture of various chemical constituents, do not understand wherein function and the mechanism of action of each composition on cell and molecular level so far as yet, so it takes effect in treatment of diseases slowly.

Yet, adopt the antibiotic therapy avian viral diseases, so not only toxic and side effects is bigger, and particularly the side effect that the hepatic and renal function of birds is produced easily causes the birds drug disposition residual; Simultaneously also with environmental pollution, and medical expense is higher.

Impurity such as the pesticide that traditional SHUANGHUANGLIAN KOUFUYE exists, heavy metal are residual.The residual problems that exceed standard of harmful substance such as the heavy metal in the Chinese crude drug, pesticide, carcinogen are not resolved always, influence taking for a long time of SHUANGHUANGLIAN KOUFUYE.

Therefore, how providing that a kind of preparation technology is simple, pollution abatement is residual, the traditional Chinese biological preparation of the SHUANGHUANGLIAN KOUFUYE of good effect, instant effect and preparation method thereof, is one of this technical field scientific research personnel new problem of being badly in need of developing.

Summary of the invention

The objective of the invention is to overcome the above-mentioned shortcoming of pure Chinese medicinal preparation, a kind of Traditional Chinese Herb active component that has is provided, molecular weight is little, is easy to enter human body cell, the traditional Chinese biological preparation of the SHUANGHUANGLIAN KOUFUYE of instant effect.

Another object of the present invention is to provide the preparation method of the traditional Chinese biological preparation of this SHUANGHUANGLIAN KOUFUYE.

The present invention is achieved through the following technical solutions: a kind of traditional Chinese biological preparation of SHUANGHUANGLIAN KOUFUYE is characterized in that microbial inoculant is carried out fermenting process of preparing in the SHUANGHUANGLIAN KOUFUYE of being made by Chinese crude drug to form.

Described SHUANGHUANGLIAN KOUFUYE comprises the composition of following proportioning by weight: Rhizoma Coptidis 10～42, Flos Lonicerae 10～50, Fructus Forsythiae 10～40, Radix Scutellariae 10～38.

Described microorganism is an EM original solution.

Described EM original solution is for containing bacillus cereus, lactobacillus, yeast, light and antibacterial.

A kind of preparation method of the traditional Chinese biological preparation according to the described SHUANGHUANGLIAN KOUFUYE of claim 1 is characterized in that comprising the steps:

(1) above-mentioned Chinese crude drug decocting being boiled and be concentrated into concentration is 0.2～2.0g/ml, makes SHUANGHUANGLIAN KOUFUYE;

(2) EM original solution is inoculated in the SHUANGHUANGLIAN KOUFUYE, 10～30 ℃ of temperature and illumination condition bottom fermentation 1～5 month, the gained medicinal liquid separated, extracts, makes with extra care, sterilizes, and makes oral formulations; Or spray drying or lyophilization make the graininess capsule, or solid preparation.

Chinese medicine mainly relies on macromole performance curative effect, the present invention combines modern biotechnology and Chinese medicine prescription, microorganism is incorporated into carries out fermentating metabolism in the SHUANGHUANGLIAN KOUFUYE, the utilization that biotransformation function by microorganism and idiotrophic are worth, in the mode of combination among the strong ones, the traditional Chinese biological preparation of preparation SHUANGHUANGLIAN KOUFUYE.

Primary raw material of the present invention is Rhizoma Coptidis, Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae.Radix Scutellariae both can be used as medicine separately, also can unite use with other medical material, to reach clinical required curative effect.Flos Lonicerae is exactly a Flos Lonicerae, but sweet in the mouth cold in nature, abnormal smells from the patient awns perfume (or spice), both fresh breeze using warming therapy heat can be separated the poison in the blood again, are applicable to epidemic febrile disease heating, macule, pharyngalgia.The Rhizoma Coptidis bitter in the mouth is cold in nature, in the removing pathogen in the heart after the fire, damp and hot in the bowel relieving, be applicable to excessive fire in heart, damp and hot, the damp and hot pestilent toxicity of the intestines and stomach.Fructus Forsythiae bitter in the mouth cool in nature, clearing heart fire goes the various heat symptom-complexs of the part of the body cavity above the diaphragm housing the heart and lungs, is applicable to epidemic febrile disease from the beginning of, headache, heating, excessive thirst, and the carbuncle skin ulcer that pyretic toxicity pent-up causes swells and ache, erysipelas, macule etc.Particularly, drug effect is brought into play more fully by the EM original solution fermentation.

Compared with the prior art, the present invention has the following advantages and effect:

1, impurity such as the pesticide of traditional SHUANGHUANGLIAN KOUFUYE existence, heavy metal are residual.The residual problems that exceed standard of harmful substance such as the heavy metal in the Chinese crude drug, pesticide, carcinogen are not resolved always, influence taking for a long time of SHUANGHUANGLIAN KOUFUYE.The present invention is incorporated into EM original solution and carries out fermentating metabolism in the SHUANGHUANGLIAN KOUFUYE, biotransformation function by EM original solution is with the heavy metal in the medical material, pesticide, carcinogen reduce or remove, and it is particularly important as the consumer of health purpose that this takes Liuwei Dihuang preparation for a long time for need.

2, owing to production technology, conventional formulation need add the molding of a large amount of adjuvants ability, so dose is big, bioavailability is low.Preparation method of the present invention is handled through microbial fermentation, and will go into the molecular substance metabolism is small-molecule active substance, has improved bioavailability, and absorption is rapid, complete, and has reduced dose.

3, the traditional Chinese biological preparation for preparing after the EM original solution metabolism of SHUANGHUANGLIAN KOUFUYE, the effect at aspects such as enhance immunity obviously strengthens, and is better than traditional SHUANGHUANGLIAN KOUFUYE.

The specific embodiment

Below in conjunction with preferred embodiment, to details are as follows according to the specific embodiment provided by the invention:

Embodiment 1

The preparation of SHUANGHUANGLIAN KOUFUYE traditional Chinese biological preparation

With Rhizoma Coptidis 20g, Flos Lonicerae 15g, Fructus Forsythiae 28g, Radix Scutellariae 10g, water decocts and is concentrated into concentration is 0.2～2.0g/ml, makes SHUANGHUANGLIAN KOUFUYE; EM original solution is inoculated in the SHUANGHUANGLIAN KOUFUYE, and 30 ℃ of temperature and illumination condition bottom fermentation 2 months, the gained medicinal liquid separates, and extracts, and was refining, and oral formulations is made in sterilization.Used EM original solution is for containing bacillus cereus, lactobacillus, yeast, light and antibacterial etc.; The preferred purple nonsulfur bacteria of EM original solution section antibacterial.

Embodiment 2

The preparation of SHUANGHUANGLIAN KOUFUYE traditional Chinese biological preparation

With Rhizoma Coptidis 15g, Flos Lonicerae 48g, Fructus Forsythiae 22g, Radix Scutellariae 25g, water decocts and is concentrated into concentration is 0.2～2.0g/ml, makes SHUANGHUANGLIAN KOUFUYE; EM original solution is inoculated in the SHUANGHUANGLIAN KOUFUYE, and 28 ℃ of temperature and illumination condition bottom fermentation 5 months, the gained medicinal liquid separates, and extracts, and was refining, sterilization, and the spray drying capsule, or solid tablet is made in lyophilization.

Other is with embodiment 1.

Chemical constitution study

SHUANGHUANGLIAN KOUFUYE added scans before and after the EM original solution, obtain mass spectrum, find: (m/z) in 50～1000 scopes, SHUANGHUANGLIAN KOUFUYE add light can antibacterial before with after fragment ion quantity and parent ion size obvious variation has taken place.Parent ion was dispersed in (m/z) 50～900 scopes before SHUANGHUANGLIAN KOUFUYE added EM original solution, it is more that parent ion often reaches fragment ion quantity: parent ion was dispersed in the scope of (m/z) 50～500 after SHUANGHUANGLIAN KOUFUYE added EM original solution, parent ion often reaches the fragment ion negligible amounts, and (m/z) does not almost have (m/z) big parent ion in 500～1000 model nets.After preliminary explanation SHUANGHUANGLIAN KOUFUYE adds EM original solution, make macromolecular complex be changed into small-molecule active substance by the metabolism of ten EM original solution

Application example

200 15 age in days broiler (aseptic through physical examination) are divided into totally 5 groups of A, B, C, D, E, test behind B, C, the strong malicious counteracting toxic substances of D, E group process artificial challenge's 0.6ml bursal disease virus of chickens.A group is healthy group, infective virus not, not administration; B organizes negative control group, infective virus, not administration; The C group is the pharmaceutical composition low dose group, presses 0.06g/ chicken/sky injection; The D group is the pharmaceutical composition high dose group, presses 0.12g/ chicken/sky injection; The E group is pressed 0.12g/ chicken/sky administration for tradition " SHUANGHUANGLIAN KOUFUYE " treatment group.The result shows, adopts the traditional Chinese biological preparation treatment chicken bursal disease viral disease of nano ' Shuang Hualian ' oral liquid of the present invention that tangible curative effect is arranged.

The traditional Chinese biological preparation contrast and experiment of SHUANGHUANGLIAN KOUFUYE of the present invention is as follows:

Numbering	Group	Mortality rate %	Cure rate %	Effective percentage %
					??A	Healthy group	??0	??97.5(39/40)	??100.0(40/40)
??B	Negative control group	??55.0(22/40)	??5.0(2/40)	??25.0(10/40)
					??C	Low dose therapy group of the present invention	??30.0(12/40)	??42.5(17/40)	??77.5(31/40)
??D	High-dose therapy group of the present invention	??20.0(8/40)	??62.5(25/40)	??87.5(35/40)
					??E	Tradition " SHUANGHUANGLIAN KOUFUYE " treatment group	??25.0(10/40)	??45.0(18/40)	??80.0(32/40)

This result of the test shows, pharmaceutical composition is low, high-dose therapy group and matched group difference highly significant (P＜0.01), illustrates that pharmaceutical composition of the present invention has significant therapeutical effect to the chicken bursal disease viral disease that virus causes; Particularly high dose is used and obviously is better than tradition " SHUANGHUANGLIAN KOUFUYE " treatment group curative effect (P＜0.01).

Above-mentioned detailed description of traditional Chinese biological preparation of this SHUANGHUANGLIAN KOUFUYE and preparation method thereof being carried out with reference to embodiment; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.

Claims (5)

1, a kind of traditional Chinese biological preparation of SHUANGHUANGLIAN KOUFUYE is characterized in that microbial inoculant is carried out fermenting process of preparing in the SHUANGHUANGLIAN KOUFUYE of being made by Chinese crude drug to form.

2, the traditional Chinese biological preparation of SHUANGHUANGLIAN KOUFUYE according to claim 1 is characterized in that described SHUANGHUANGLIAN KOUFUYE comprises the composition of following proportioning by weight: Rhizoma Coptidis 10～42, Flos Lonicerae 10～50, Fructus Forsythiae 10～40, Radix Scutellariae 10～38.

3, the traditional Chinese biological preparation of SHUANGHUANGLIAN KOUFUYE according to claim 1 is characterized in that described microorganism is an EM original solution.

4, the traditional Chinese biological preparation of SHUANGHUANGLIAN KOUFUYE according to claim 3 is characterized in that described EM original solution is for containing bacillus cereus, lactobacillus, yeast, light and antibacterial.

5, a kind of preparation method of the traditional Chinese biological preparation according to the described SHUANGHUANGLIAN KOUFUYE of claim 1 is characterized in that comprising the steps:

(1) above-mentioned Chinese crude drug decocting being boiled and be concentrated into concentration is 0.2～2.0g/ml, makes SHUANGHUANGLIAN KOUFUYE;

(2) EM original solution is inoculated in the SHUANGHUANGLIAN KOUFUYE, 10～30 ℃ of temperature and illumination condition bottom fermentation 1～5 month, the gained medicinal liquid separated, extracts, makes with extra care, sterilizes, and makes oral formulations; Or spray drying or lyophilization make the graininess capsule, or solid preparation.