CN105747231A - Technology for extracting and preparing starch inhibitor from white kidney beans - Google Patents
Technology for extracting and preparing starch inhibitor from white kidney beans Download PDFInfo
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- CN105747231A CN105747231A CN201610150957.3A CN201610150957A CN105747231A CN 105747231 A CN105747231 A CN 105747231A CN 201610150957 A CN201610150957 A CN 201610150957A CN 105747231 A CN105747231 A CN 105747231A
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- 229920002472 Starch Polymers 0.000 title claims abstract description 65
- 235000019698 starch Nutrition 0.000 title claims abstract description 65
- 239000008107 starch Substances 0.000 title claims abstract description 65
- 239000003112 inhibitor Substances 0.000 title claims abstract description 37
- 244000046052 Phaseolus vulgaris Species 0.000 title abstract description 16
- 238000005516 engineering process Methods 0.000 title abstract description 12
- 235000021332 kidney beans Nutrition 0.000 title abstract 6
- 239000000284 extract Substances 0.000 claims abstract description 37
- 239000006228 supernatant Substances 0.000 claims abstract description 36
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 26
- 239000004375 Dextrin Substances 0.000 claims abstract description 23
- 229920001353 Dextrin Polymers 0.000 claims abstract description 23
- 235000019425 dextrin Nutrition 0.000 claims abstract description 23
- 229920002678 cellulose Polymers 0.000 claims abstract description 21
- 239000001913 cellulose Substances 0.000 claims abstract description 21
- 102000004190 Enzymes Human genes 0.000 claims abstract description 20
- 108090000790 Enzymes Proteins 0.000 claims abstract description 20
- 238000001694 spray drying Methods 0.000 claims abstract description 19
- 239000011259 mixed solution Substances 0.000 claims abstract description 17
- 108010019077 beta-Amylase Proteins 0.000 claims abstract description 14
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 244000013123 dwarf bean Species 0.000 claims description 93
- 235000021278 navy bean Nutrition 0.000 claims description 93
- 238000000034 method Methods 0.000 claims description 50
- 239000007789 gas Substances 0.000 claims description 37
- 238000002360 preparation method Methods 0.000 claims description 34
- 238000000605 extraction Methods 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000012141 concentrate Substances 0.000 claims description 23
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- 230000033228 biological regulation Effects 0.000 claims description 11
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- 238000003809 water extraction Methods 0.000 abstract description 3
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- 244000005700 microbiome Species 0.000 abstract description 2
- 230000000087 stabilizing effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- 238000000227 grinding Methods 0.000 abstract 1
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
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- 206010020772 Hypertension Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
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- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 201000002451 Overnutrition Diseases 0.000 description 1
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- 241001119522 Paullinia pinnata Species 0.000 description 1
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- 108090000284 Pepsin A Proteins 0.000 description 1
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- FRYDSOYOHWGSMD-UHFFFAOYSA-N [C].O Chemical group [C].O FRYDSOYOHWGSMD-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a technology for extracting and preparing a starch inhibitor from white kidney beans.The technology includes subjecting the white kidney beans to microwave sterilization, grinding prior to sieving and water extraction, and filtering filter residues to obtain a liquid supernatant; on a basis of total content of cellulose and starch in the liquid supernatant, adding 1-2% of cellulase, 1.5-2.5% of alpha-amylase and 3-5% of beta-amylase to react; conducting vacuum concentration to obtain a concentrated solution and regulating a pH (potential of hydrogen) value; adding resistant dextrin into the concentrated solution and uniformly mixing so as to obtain a mixed solution; subjecting the mixed solution to low-temperature spray drying so as to obtain the starch inhibitor.The technology for extracting and preparing the starch inhibitor from the white kidney beans combines enzyme treatment with microwave treatment to effectively remove impurities and microorganisms of the selected raw materials and the ingredients such as the cellulose and the starch and extracts the starch inhibitor from the white kidney beans through the technologies such as microwave sterilization, combination of water extraction and enzyme treatment and low-temperature spray drying.Meanwhile, the resistant dextrin is combined to enhance blood sugar stabilizing effect, and white kidney beans extract is developed and utilized.
Description
Technical field
The present invention relates to food and extract preparation field.More particularly, to from raw material select and pre-treatment,
Extract, be dried the extraction and preparation technique of starch Inhibitors in a kind of navy bean of totally three links.
Background technology
In recent years along with the fast development of China's economic, national income gradually steps up, the meals water of resident
Put down and be also correspondingly improved, occurred in that the phenomenon of overnutrition.On the other hand, laboring form also exists
Being gradated as mechanization by craftization, pencil pusher's ratio is gradually increasing, and the work of worker is strong
Degree gradually decreases.Environment, pressure and bad life habits in addition on the basis of both the above principal element
Make etc. factor the diseases such as obesity, hypertension, high fat of blood, diabetes fall ill send out a rate rise the most year by year,
Cause the attention of society's various fields such as traditional Chinese medicine, food.But, the disease of these diseases sends out reason
It is a complicated mechanism, the most also interacts between various diseases.Human body prevents this
The most direct mode of class disease is from the beginning of diet, and in the food that the mankind daily intake, major part is carbon
Hydrate, lives in vivo biodistribution such as ptyalin, hydrochloric acid in gastric juice, pepsin, trypsase after eating
Under property composition effect, it is eventually converted into monose through series reaction, digested entrance blood,
Transport, to each organ sites generation redox reaction, releases energy, it is ensured that human body is the most metabolic
Required energy；Another part, is converted into muscle glycogen, is stored among human body.Required for human body every day
Sugar amount be limited, the amount of amount and consumption sugar that every day stores sugar is among dynamic equilibrium, if taken the photograph
Entering too much carbohydrate, monose unnecessary in health will increase and even cause some diseases.One side
Face, human body is taken in too much carbohydrate and is become monose, a part to provide people its enzymolysis through digestive system
Body sustains life required energy, is then converted into fat accumulation for monose remaining after human body requirements
Get off, cause the diseases such as obesity；On the other hand, internal pancreas islet will be caused in too much monose body
Element steeply rises, and causes internal insulin metabolism disorderly, even increases the probability suffering from the diseases such as diabetes.
It follows that keep blood sugar to be in stable state, then can to reduce the generation of the disease such as obesity, diabetes several
Rate, contributes to the mankind and has the health of health.
On the basis of ensureing human homergy, the most effective intake controlling monose, remain stable
Blood sugar level be human body maintain normal body level simple and effective way, how to maintain blood glucose balance
Being a underlying issue ensureing human body health status, in navy bean, extract is permissible as a kind of glycoprotein
The activity of suppression alpha amylase reduces the decomposition of carbohydrate, thus reduces human body to carbohydrate
Digestion and absorption, reduce the quantity of internal acquisition monose, reaches to a certain extent to control blood glucose balance
Effect.In recent years, the crowd of research navy bean extract gets more and more, as far back as on March 16th, 2011,
Navy bean extract is just approved as bread and cheese raw material by Ministry of Health of the People's Republic of China, the most domestic not
It is a series of main based on solid beverage that few enterprise has selected navy bean extract to be that primary raw material is developed
Want the weight management products of formulation.The research of external dialogue Semen Phaseoli Vulgaris extract is the most extensive, and product form is also
Not to be restricted to one pattern, from the pulvis of instant edible, tablet to delicious and nourishing energy stick again to good to eat pleasant
Coffee, the product form such as good to eat, extensively favored by consumer.In addition, dialogue Semen Phaseoli Vulgaris extract
The both effectiveness of product is also carried out checking, and Scranton university of the U.S. finds to take navy bean by human trial
The level of postprandial blood sugar of the experimenter of extract can decline 66%, the researcher of Kato Li Ka university of Italy
Find to continue within 30 days, to take the experimenter of 445mg navy bean extract in muscle groups again by human trial
Knitting on the basis of not changing, body weight can reduce 3kg, and waistline can reduce 3cm.Zhejiang University cures
After the effect of institute's also dialogue Semen Phaseoli Vulgaris extract carries out verifying and find to take navy bean extract 60 days, it is subject to
Body weight and the body-mass index of examination person are the most on a declining curve.
Navy bean extract can effectively stop starch to decompose, and belongs to a kind of glycoprotein, and sugar chain has height
Hydrophily, be highly soluble in water.In navy bean, this type of glycoprotein Content is limited, if only eating navy bean very
Difficulty plays the diastatic effect of suppression, it is impossible to reaches the effect controlling blood sugar with body weight, concentrates through extracting
After navy bean extract in this type of glycoprotein Content rise, have and significantly suppress amylase activity, control
The effect of blood sugar processed.But, non-refractory, necessarily avoid during extraction that temperature is too high to be caused obtaining
The reduction of extract activity even loses.
Obtaining security navy bean extract the most efficiently is to carry for the more rich navy bean of exploitation further
Take the key point of thing.Accordingly, it is desirable to provide the extraction of starch Inhibitors in the navy bean of a kind of highly effective and safe
Preparation technology.
Summary of the invention
It is an object of the present invention to provide the extraction and preparation technique of starch Inhibitors in a kind of navy bean.
Starch inhibitor prepared by this method not only has the effect that suppression starch absorbs, and additionally assists in maintaining enteron aisle bacterium
Group's balance, the effect relaxed bowel, meanwhile, the present invention selects resistant dextrin to be auxiliary material, Synergistic intestines
Road colony balance is healthy.
For reaching above-mentioned purpose, the present invention uses following technical proposals:
1, the extraction and preparation technique of starch Inhibitors in a kind of navy bean, comprises the steps:
1) choose navy bean and carry out microwave disinfection；
2) being pulverized and sieved by the navy bean after microwave disinfection, addition water carries out water and carries, and takes after filtering filter residue
Clear liquid, repeats to extract filter residue repeatedly according to above-mentioned steps, merges supernatant；
3) take the supernatant after merging, wherein fiber content, content of starch are measured；
4) according to measurement result, in terms of the total content of cellulose in supernatant and starch, fine according to 1-2%
Dimension element enzyme, 1.5-2.5% AMS, the ratio of 3-5% beta amylase add three kinds of enzymes, react；
5) take reacted supernatant and be concentrated under reduced pressure to give concentrate, regulate pH；
6) in concentrate, add resistant dextrin, mix and obtain mixed solution；
7) above-mentioned mixed solution is carried out low temperature spray drying, i.e. obtain final products.
Preferably, step 1) in, the power of microwave disinfection is 200-250W, and the time of microwave disinfection is
30-45s, to ensure the cleannes of raw material.
Preferably, step 2) in, navy bean crosses 60 mesh sieves after pulverizing, and water puies forward the addition of process water and is
The navy bean of 15 times of volumes, the temperature that water carries is 55-60 DEG C, and the time that water carries is 11-12h.Carry at this
Under the conditions of taking, the material in navy bean can be substantially soluble among extract.
Preferably, step 4) in, once add fiber according to the ratio of 1-2%, 1.5-2.5%, 3-5%
Element enzyme, AMS, beta amylase, the temperature of reaction is 70 DEG C, and the time of reaction is 50-60min,
With the starch in removal extract, cellulose.Meanwhile, the amount of added enzyme is in strict accordance with in raw material
Fiber, content of starch determine, it is to avoid the enzyme operation of going out needed for adding excess because of enzyme, live in extract
Property composition has certain protective effect.
Preferably, step 5) in, reacted supernatant is evaporated to solid content and reaches 20-25%.
Selecting so to concentrate scope, be to consider both sides factor: on the one hand, in card extract, activity becomes
Point, be on the other hand conducive to subsequent spray drying process.
Preferably, step 5) in, the pH of regulation concentrate is 6.2-6.5.The selection of this pH scope is also
It is to consider both sides factor: most useful for the extraction of starch inhibitor, the most beneficially resistant dextrin
It is uniformly distributed.
Preferably, step 6) in, the addition of resistant dextrin is the 7% of concentrate.The choosing of this addition
Select the reason of three aspects: reach the purpose that assistant spray is dried, improve extract suppression alphalise starch simultaneously
The effect of enzymatic activity, and contribute to maintaining gut flora balance.
Preferably, step 7) in, the condition of low temperature spray drying is input gas temperature 70-80 DEG C, pressure
Contracting gas flow 400-450L/h, flows of dry gases 0.88-0.9m3/min.Through substantial amounts of experimental verification,
The loss of activity of navy bean extract can be reduced under the conditions of this.
Further, described preparation technology comprises the following specific steps that:
1) choose navy bean and carry out microwave disinfection；
2) navy bean after microwave disinfection was pulverized 60 mesh sieves, added the water of 15 times of volumes at 55-60 DEG C
Carry out water to carry, extract 12h, after filtering filter residue, take supernatant, repeat to extract filter residue 2 according to above-mentioned steps
Secondary, merge supernatant；
3) take the supernatant after merging, wherein fiber content, content of starch are measured；
4) according to measurement result, in terms of the total content of cellulose in supernatant and starch, fine according to 1-2%
Dimension element enzyme, 1.5-2.5% AMS, the ratio of 3-5% beta amylase add three kinds of enzymes, react at 70 DEG C
50-60min；
5) take reacted supernatant to be evaporated to solid content and reach 20-25% and obtain concentrate,
PH is between 6.2-6.5 in regulation；
6) in concentrate, add the resistant dextrin of 7%, mix and obtain mixed solution；
7) to above-mentioned mixed solution input gas temperature 70-80 DEG C, compression gas flow 400-450L/h,
Flows of dry gases 0.88-0.9m3It is spray-dried under the conditions of/min, i.e. obtains final products.
In prior art, it is mainly water extraction and organic for the extracting method of starch Inhibitors in navy bean
Extraction method.Such as patent CN103638089A is announced one and is utilized microwave-complex enzyme coupled method from navy bean
The method of middle extraction alpha-amylase inhibitor, but its shortcoming is to operate the most loaded down with trivial details, process conditions requirement
Strictly, enzyme concentration determines according to navy bean sum, and enzyme addition is difficult to moderate needs to be carried out going out ferment treatment,
Active component in extract is had a certain impact；And for example patent CN201510135921 is announced a kind of white
The extracting method of kidney bean alpha-amylase inhibitor, but it does not carries out sterilization treatment to navy bean, and raw material cleans
Spending the most secure, and introduce ethanol in alcohol precipitation process, the security on extract has certain impact.
Ferment treatment is mainly combined by the inventive method with microwave treatment method, effectively removes the miscellaneous of selected raw material
Matter, microorganism and the composition such as cellulose, starch, use microwave disinfection (200-250W microwave disinfection 30-45s
(add 15 long-pending water at 55-60 DEG C, extract 11-12h), ferment treatment (1-2% cellulase, 1.5-2.5%
AMS, 3-5% beta amylase, at 70 DEG C process 50-60min), low temperature spray drying (input gas
Temperature 70-80 DEG C, compressed air require 400-450L/h, flows of dry gases 0.88-0.9m3/min)
Extracting starch inhibitor in navy bean etc. technique, in combination with resistant dextrin, (addition is concentrate
7%), increase effect of this product stabilizing blood sugar, the beneficially development and utilization of navy bean extract.
Beneficial effects of the present invention is as follows:
1. use microwave disinfection (200-250 microwave disinfection 30-45s), it is ensured that navy bean extract sanitary index
Qualified.
2. determine enzyme addition (1-2% cellulase, 1.5-2.5% α-shallow lake according to cellulose, content of starch
Powder enzyme, 3-5% beta amylase, process 50-60min at 70 DEG C), it is to avoid high temperature goes out and causes in enzyme process forming sediment
Powder inhibitor activity weakens.
3. select in weak acid environment and extract (pH controls at 6.2-6.5), the beneficially extraction of starch inhibitor,
Be conducive to being uniformly distributed of resistant dextrin simultaneously.
4. select resistant dextrin (addition is the 7% of concentrate) to carry out auxiliary to be dried, increase extract dimension
The effect hold gut flora balance, relaxing bowel.
5. (low temperature spray drying condition is input gas temperature 70-80 DEG C, pressure to select low temperature spray drying method
Contracting gas flow 400-450L/h, flows of dry gases is 0.88-0.9m3/ min, reduces navy bean extract
Loss of activity.
Accompanying drawing explanation
Below in conjunction with the accompanying drawings the detailed description of the invention of the present invention is described in further detail.
Fig. 1 illustrates that according to the method for testing of checking one, preparation technology of the present invention being extracted prepared navy bean carries
Take the experimental result that thing carries out suppressing amylase activity to measure.
Fig. 2 illustrates that according to the method for testing of checking two, preparation technology of the present invention being extracted prepared navy bean carries
Take the experimental result that thing carries out suppressing amylase activity to measure.
Detailed description of the invention
In order to be illustrated more clearly that the present invention, below in conjunction with preferred embodiments and drawings, the present invention is done into one
The explanation of step.Parts similar in accompanying drawing are indicated with identical reference.Those skilled in the art
Should be appreciated that following specifically described content is illustrative and be not restrictive, should not limit with this
Protection scope of the present invention.
Embodiment 1
In a kind of navy bean, the extraction and preparation technique of starch Inhibitors, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 200W power, microwave 45s.
4th step, the navy bean after sterilization being completed grinds, and crosses 60 mesh sieves, adds 15 times of 55 DEG C of water, carry
Take 12h, by 3 layers of filtered through gauze, take supernatant, according to above method, filter residue is carried out 2 times and extract, by 3
Secondary filtrate mixing.
5th step, takes above-mentioned filtrate 10mL, with reference to standard GB/T/T5515-2008, GB/T5515-2008
Cellulose, starch in filtrate are measured.
6th step, according to cellulose, the content of starch in above-mentioned surveyed filtrate, add 1% cellulase,
1.5% AMS and 3% beta amylase, react 60min under the conditions of 70 DEG C.
7th step, is evaporated to solid content by above-mentioned mixing supernatant under the conditions of 30 DEG C and reaches 20%,
PH is between 6.2-6.5 in regulation；
8th step, the ratio according to 7% adds resistant dextrin in above-mentioned concentrate, mixing；
9th step, is 80 DEG C in input gas temperature, and compression gas flow is 400L/h, is dried gas stream
Amount is 0.88m3Under the conditions of/min, above-mentioned mixed solution is carried out low temperature spray drying.
Embodiment 2
In a kind of navy bean, the extraction and preparation technique of starch Inhibitors, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 250W power, microwave 30s.
4th step, the navy bean after sterilization being completed grinds, and crosses 60 mesh sieves, adds 15 times of 60 DEG C of water, carry
Take 11h, by 3 layers of filtered through gauze, take supernatant, according to above method, filter residue is carried out 2 times and extract, by 3
Secondary filtrate mixing.
5th step, takes above-mentioned filtrate 10mL, with reference to standard GB/T/T5515-2008, GB/T5515-2008
Cellulose, starch in filtrate are measured.
6th step, according to cellulose, the content of starch in above-mentioned surveyed filtrate, add 2% cellulase,
2.5% AMS and 5% beta amylase, react 50min under the conditions of 70 DEG C.
7th step, is evaporated to solid content by above-mentioned mixing supernatant under the conditions of 30 DEG C and reaches 20%,
PH is between 6.2-6.5 in regulation；
8th step, the ratio according to 7% adds resistant dextrin in above-mentioned concentrate, mixing；
9th step, is 70 DEG C in input gas temperature, and compression gas flow is 420L/h, is dried gas stream
Amount is 0.9m3Under the conditions of/min, above-mentioned mixed solution is carried out low temperature spray drying.
Embodiment 3
In a kind of navy bean, the extraction and preparation technique of starch Inhibitors, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 200W power, microwave 45s.
4th step, the navy bean after sterilization being completed grinds, and crosses 60 mesh sieves, adds 15 times of 60 DEG C of water, carry
Take 12h, by 3 layers of filtered through gauze, take supernatant, according to above method, filter residue is carried out 2 times and extract, by 3
Secondary filtrate mixing.
5th step, takes above-mentioned filtrate 10mL, with reference to standard GB/T/T5515-2008, GB/T5515-2008
Cellulose, starch in filtrate are measured.
6th step, according to cellulose, the content of starch in above-mentioned surveyed filtrate, add 1.5% cellulase,
2% AMS and 4% beta amylase, react 50min under the conditions of 70 DEG C.
7th step, is evaporated to solid content by above-mentioned mixing supernatant under the conditions of 30 DEG C and reaches 20%,
PH is between 6.2-6.5 in regulation；
8th step, the ratio according to 7% adds resistant dextrin in above-mentioned concentrate, mixing；
9th step, is 80 DEG C in input gas temperature, and compression gas flow is 400L/h, is dried gas stream
Amount is 0.88m3Under the conditions of/min, above-mentioned mixed solution is carried out low temperature spray drying.
Embodiment 4
In a kind of navy bean, the extraction and preparation technique of starch Inhibitors, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 200W power, microwave 45s.
4th step, the navy bean after sterilization being completed grinds, and crosses 60 mesh sieves, adds 15 times of 55 DEG C of water, carry
Take 12h, by 3 layers of filtered through gauze, take supernatant, according to above method, filter residue is carried out 2 times and extract, by 3
Secondary filtrate mixing.
5th step, takes above-mentioned filtrate 10mL, with reference to standard GB/T/T5515-2008, GB/T5515-2008
Cellulose, starch in filtrate are measured.
6th step, according to cellulose, the content of starch in above-mentioned surveyed filtrate, add 1.5% cellulase,
2.5% AMS and 3.5% beta amylase, react 50min under the conditions of 70 DEG C.
7th step, is evaporated to solid content by above-mentioned mixing supernatant under the conditions of 30 DEG C and reaches 25%,
PH is between 6.2-6.5 in regulation；
8th step, the ratio according to 7% adds resistant dextrin in above-mentioned concentrate, mixing；
9th step, is 80 DEG C in input gas temperature, and compression gas flow is 450L/h, is dried gas stream
Amount is 0.88m3Under the conditions of/min, above-mentioned mixed solution is carried out low temperature spray drying.
Comparative example 1
The preparation technology of a kind of navy bean powder, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 200W power, microwave 45s.
4th step, the navy bean after cleaning grinds, and 60 mesh sieves excessively obtain navy bean powder and are designated as contrasting extract
1。
Comparative example 2
In a kind of navy bean, the extraction and preparation technique of starch Inhibitors, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 200W power, microwave 45s.
4th step, the navy bean after sterilization being completed grinds, and crosses 60 mesh sieves, adds 15 times of 55 DEG C of water, carry
Take 12h, by 3 layers of filtered through gauze, take supernatant, according to above method, filter residue is carried out 2 times and extract, by 3
Secondary filtrate mixing.
5th step, is evaporated to solid content by above-mentioned mixing supernatant under the conditions of 30 DEG C and reaches 20%,
PH is between 6.2-6.5 in regulation；
6th step, the ratio according to 7% adds resistant dextrin in above-mentioned concentrate, mixing；
7th step, is 80 DEG C in input gas temperature, and compression gas flow is 400L/h, is dried gas stream
Amount is 0.88m3Under the conditions of/min, above-mentioned mixed solution is carried out low temperature spray drying.Obtained by without
The navy bean starch Inhibitors extract of ferment treatment is designated as contrasting extract 2.
Comparative example 3
In a kind of navy bean, the extraction and preparation technique of starch Inhibitors, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 200W power, microwave 45s.
4th step, the navy bean after sterilization being completed grinds, and crosses 60 mesh sieves, adds 15 times of 55 DEG C of water, carry
Take 12h, by 3 layers of filtered through gauze, take supernatant, according to above method, filter residue is carried out 2 times and extract, by 3
Secondary filtrate mixing.
5th step, takes above-mentioned filtrate 10mL, with reference to standard GB/T/T5515-2008, GB/T5515-2008
Cellulose, starch in filtrate are measured.
6th step, according to cellulose, the content of starch in above-mentioned surveyed filtrate, add 1% cellulase,
1.5% AMS and 3% beta amylase, react 60min under the conditions of 70 DEG C.
7th step, is evaporated to solid content by above-mentioned mixing supernatant under the conditions of 30 DEG C and reaches 20%,
PH is between 6.2-6.5 in regulation；
8th step, the ratio according to 7% adds common dextrin in above-mentioned concentrate, mixing；
9th step, is 80 DEG C in input gas temperature, and compression gas flow is 400L/h, is dried gas stream
Amount is 0.88m3Under the conditions of/min, above-mentioned mixed solution is carried out low temperature spray drying.Obtained by not with
The navy bean starch Inhibitors extract that resistant dextrin is combined is designated as contrasting extract 3.
Comparative example 4
In a kind of navy bean, the extraction and preparation technique of starch Inhibitors, comprises the steps:
1st step, chooses the high quality white kidney bean 200 grams of North America import, with the sodium hydrogensulfite of 0.2%, incites somebody to action
It is cleaned.
2nd step, dries it under the conditions of 60 DEG C to moisture less than 1%.
3rd step, is placed in micro-wave oven, with 200W power, microwave 45s.
4th step, the navy bean after sterilization being completed grinds, and crosses 60 mesh sieves, adds 15 times of 55 DEG C of water, carry
Take 12h, by 3 layers of filtered through gauze, take supernatant, according to above method, filter residue is carried out 2 times and extract, by 3
Secondary filtrate mixing.
5th step, takes above-mentioned filtrate 10mL, with reference to standard GB/T/T5515-2008, GB/T5515-2008
Cellulose, starch in filtrate are measured.
6th step, according to cellulose, the content of starch in above-mentioned surveyed filtrate, add 1% cellulase,
1.5% AMS and 3% beta amylase, react 60min under the conditions of 70 DEG C.
7th step, is evaporated to solid content by above-mentioned mixing supernatant under the conditions of 30 DEG C and reaches 20%,
PH is between 6.2-6.5 in regulation；
8th step, the ratio according to 7% adds common dextrin in above-mentioned concentrate, mixing；
9th step, is 160 DEG C in input gas temperature, and compression gas flow is 400L/h, is dried gas
Flow is 0.88m3Under the conditions of/min, above-mentioned mixed solution is spray-dried.Obtained is provided without
The navy bean starch Inhibitors extract that low temperature spray drying technique prepares is designated as contrasting extract 4.
Navy bean and navy bean extract suppression amylase activity measure
Carry out suppressing amylase activity to carry out to extracting, according to above-described embodiment 1, the navy bean extract prepared
Measuring, the navy bean extract extracted with navy bean and comparative example is as control group.
Checking one
The navy bean extract prepared using embodiment 1 is as test group 1；Prepare with embodiment 2
Navy bean extract as test group 2；The navy bean extract prepared using embodiment 3 is as test
Group 3；The navy bean extract prepared using embodiment 4 is as test group 4, to above four kinds of embodiment party
The starch inhibitor activity that method prepares is measured.
Checking two
The highest sample of suppression amylase activity is obtained for test group 1 in 4 embodiments；Comparative example
The navy bean powder that 1 prepares is as test group 2；It is provided without enzymolysis process with what comparative example 2 prepared
The navy bean extract prepared is as test group 3；It is provided without resistant dextrin with what comparative example 3 prepared
Navy bean extract is as test group 4；It is provided without low temperature spray drying technology with what comparative example 4 prepared
Obtained navy bean extract is as test group 5.The suppression amylase activity of the samples of above five kinds is entered
Row measures.
Test method:
Checking one
Choosing the Healthy People in 100 28-50 years, wherein 10 male 10 female are one group, are divided into five groups.
Test crowd takes in 100g rice in the case of not taking food simultaneously, and test group 1 is clothes half an hour before ingesting
With the navy bean extract of 2g embodiment 1 preparation, test group 2 takes the white rue of 2g embodiment 2 preparation
Beans extract, test group 3 take 2g embodiment 3 preparation navy bean extract, test group 4 takes 2g
The navy bean extract of embodiment 4 preparation, test group 5 is control group, does not eat any sample.To institute
Have experimenter postprandial blood sugar and in two hours after the meal the blood sugar in 20min be measured, statistical test
Result.
Checking two
Choosing the Healthy People in 120 28-50 years, wherein 10 male 10 female are one group, are divided into six groups.
Test crowd takes in 100g rice in the case of not taking food simultaneously, and test group 1 is clothes half an hour before ingesting
Suppressing, in 4 embodiments, the sample that amylase activity is the highest with in 2g checking one, test group 2 is taken
The navy bean extract of 2g comparative example 1 preparation, test group 3 is taken the navy bean of 2g comparative example 2 preparation and is carried
Taking thing, test group 4 takes the navy bean extract of 2g comparative example 3 preparation, and test group 5 takes 2g pair
The navy bean extract of ratio 4 preparation, test group 6 is control group, does not take any sample.To all
The postprandial blood sugar of experimenter and in two hours after the meal the blood sugar in 20min be measured, statistical test is tied
Really.
Result of the test:
The experimental result of checking one is as shown in Figure 1.From result, compared with test group 5, according to reality
Execute example 1-4 prepared starch inhibitor and test crowd's postprandial blood sugar change is had certain stabilization, its
In the sample for preparing according to embodiment 3 stabilization that postprandial blood sugar is changed optimal.
Verify two test results as shown in Figure 2.From result, compared with test group 6, test 2 groups
The crowd's postprandial blood sugar change i.e. using navy bean powder is little, and other four groups test crowd's postprandial blood sugars have
Certain change, fails to reach to control postprandial blood sugar rising it follows that only eat navy bean, slows down blood
The effect of sugar change, the amylase inhibitor prepared according to extracting method described in patent of the present invention can play
Suppression postprandial blood sugar changes to obtain effect.In addition, test group 1 gained sample suppression postprandial blood sugar change
Effect be better than the test group 3 without enzymolysis, the test group 4 not using resistant dextrin and be provided without low temperature
The test group 5 being spray-dried.
Obviously, the above embodiment of the present invention is only for clearly demonstrating example of the present invention, and
It is not the restriction to embodiments of the present invention, for those of ordinary skill in the field,
Can also make other changes in different forms on the basis of described above, here cannot be to all
Embodiment give exhaustive, every belong to the obvious change that technical scheme extended out
Change or change the row still in protection scope of the present invention.
Claims (9)
1. the extraction and preparation technique of starch Inhibitors in a navy bean, it is characterised in that include walking as follows
Rapid:
1) choose navy bean and carry out microwave disinfection；
2) being pulverized and sieved by the navy bean after microwave disinfection, addition water carries out water and carries, and takes after filtering filter residue
Clear liquid, repeats to extract filter residue repeatedly according to above-mentioned steps, merges supernatant；
3) take the supernatant after merging, wherein fiber content, content of starch are measured；
4) according to measurement result, in terms of the total content of cellulose in supernatant and starch, fine according to 1-2%
Dimension element enzyme, 1.5-2.5% AMS, the ratio of 3-5% beta amylase add three kinds of enzymes, react；
5) take reacted supernatant and be concentrated under reduced pressure to give concentrate, regulate pH；
6) in concentrate, add resistant dextrin, mix and obtain mixed solution；
7) above-mentioned mixed solution is carried out low temperature spray drying, i.e. obtain final products.
The extraction and preparation technique of starch Inhibitors in navy bean the most according to claim 1, its feature
It is: step 1) in, the power of microwave disinfection is 200-250W, and the time of microwave disinfection is 30-45s.
The extraction and preparation technique of starch Inhibitors in navy bean the most according to claim 1, its feature
It is: step 2) in, navy bean crosses 60 mesh sieves after pulverizing, and it is 15 times of bodies that water puies forward the addition of process water
Long-pending navy bean, the temperature that water carries is 55-60 DEG C, and the time that water carries is 11-12h.
The extraction and preparation technique of starch Inhibitors in navy bean the most according to claim 1, its feature
It is: step 4) in, the temperature of reaction is 70 DEG C, and the time of reaction is 50-60min.
The extraction and preparation technique of starch Inhibitors in navy bean the most according to claim 1, its feature
It is: step 5) in, reacted supernatant is evaporated to solid content and reaches 20-25%.
The extraction and preparation technique of starch Inhibitors in navy bean the most according to claim 1, its feature
It is: step 5) in, the pH of regulation concentrate is 6.2-6.5.
The extraction and preparation technique of starch Inhibitors in navy bean the most according to claim 1, its feature
It is: step 6) in, the addition of resistant dextrin is the 7% of concentrate.
The extraction and preparation technique of starch Inhibitors in navy bean the most according to claim 1, its feature
It is: step 7) in, the condition of low temperature spray drying is input gas temperature 70-80 DEG C, compressed gas
Flow 400-450L/h, flows of dry gases 0.88-0.9m3/min。
9. according to the extraction and preparation technique of starch Inhibitors in the arbitrary described navy bean of claim 1-8,
It is characterized in that, comprise the following specific steps that:
1) choose navy bean and carry out microwave disinfection；
2) navy bean after microwave disinfection was pulverized 60 mesh sieves, added the water of 15 times of volumes at 55-60 DEG C
Carry out water to carry, extract 12h, after filtering filter residue, take supernatant, repeat to extract filter residue 2 according to above-mentioned steps
Secondary, merge supernatant；
3) take the supernatant after merging, wherein fiber content, content of starch are measured；
4) according to measurement result, in terms of the total content of cellulose in supernatant and starch, fine according to 1-2%
Dimension element enzyme, 1.5-2.5% AMS, the ratio of 3-5% beta amylase add three kinds of enzymes, react at 70 DEG C
50-60min；
5) take reacted supernatant to be evaporated to solid content and reach 20-25% and obtain concentrate,
PH is between 6.2-6.5 in regulation；
6) in concentrate, add the resistant dextrin of 7%, mix and obtain mixed solution；
7) to above-mentioned mixed solution in input gas temperature 70-80 DEG C, compressed air require 400-450L/h,
Flows of dry gases 0.88-0.9m3Carry out low temperature spray drying under the conditions of/min, i.e. obtain final products.
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| CN114306419A (en) * | 2021-12-27 | 2022-04-12 | 无锡江大百泰科技有限公司 | Method for extracting alpha-amylase inhibitor from white kidney beans |
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