CN114539349A - Extraction process of nostoc proteins - Google Patents
Extraction process of nostoc proteins Download PDFInfo
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- CN114539349A CN114539349A CN202210235699.4A CN202210235699A CN114539349A CN 114539349 A CN114539349 A CN 114539349A CN 202210235699 A CN202210235699 A CN 202210235699A CN 114539349 A CN114539349 A CN 114539349A
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- Prior art keywords
- nostoc
- time
- proteins
- extraction process
- protein
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Abstract
The invention discloses an extraction process of nostoc proteins, which relates to the technical field of biological processing and comprises the following steps: pre-treating; drying and grinding; stirring; homogenizing; centrifuging; vacuum freeze drying; and (5) storing. Simplifies the operation difficulty, overcomes the harsh conditions, has far better extraction rate than the prior art, and can extract about 70 percent of protein in the nostoc. The process has the advantages of mild conditions, no phase change, low energy consumption, no need of introducing inorganic salt ions, simple and convenient operation, short time consumption, easy storage and large-scale production. The protein obtained by adopting the freeze drying method is easier to be actually stored, and a convenient, quick and easy-to-store method is provided for industrially applying the nostoc proteins; homogenizing for multiple times to break cell wall of Nostoc, adding purified water as solvent to extract Nostoc protein, increasing protein extraction efficiency, and greatly improving the retention of Nostoc holoprotein.
Description
Technical Field
The invention relates to the technical field of biological processing, in particular to a nostoc protein extraction process.
Background
The common nostoc has a wide living range, is rich in resources in China, has extremely strong vitality, can restore the vitality immediately after meeting water even if being dormant for decades, and has high nutritional value, and the content of crude fat is 0.2-4.28%; the total sugar content is 0.53-23.8%; also contains various nutritional components such as trehalose, rhamnose, pectic polysaccharides, glucoside, carotene, phycocyanin and exopolysaccharide; the protein content generally accounts for 14.6-21.81% of the dry weight, the protein content of some types even accounts for 22.8-28.7% of the dry weight, the protein content is higher than that of the Lentinus edodes, the black fungus and the tremella, the protein content also contains 18 amino acids, the total amino acid content is higher than that of the agaric and the hericium erinaceus, the protein content is similar to that of the hair weeds and the lentinus edodes, and the protein has a very high development and utilization prospect. At present, few research reports on the function of nostoc proteins are reported.
Disclosure of Invention
The invention aims to provide an extraction process of nostoc proteins, which is simple in process and low in cost, has the function of promoting plant growth and has multiple application fields.
In order to achieve the purpose, the invention provides the following technical scheme: an extraction process of nostoc proteins comprises the following steps:
(1) pretreatment: taking 3 parts of nostoc, and putting the nostoc into water to clean and remove impurities for later use;
(2) drying and grinding: draining the pretreated nostoc for 3-4 h, and drying in a dryer for 30-50 min at 55-70 ℃; grinding and crushing the dried nostoc and sieving the crushed nostoc by a 70-mesh sieve; grinding, adding purified water, and mixing at a ratio of 1: 30;
(3) stirring: the mixed solution is placed into a stirrer to be stirred at room temperature, the stirring power is 0.70-0.90 kw/m3, and the stirring time is 10-20 min;
(4) homogenizing: placing the mixed solution into a homogenizer, homogenizing for 1-3 times under 30MPa, wherein the time for homogenizing for each time is 2-4 minutes, and obtaining a homogenized solution;
(5) centrifuging: putting the homogeneous liquid into a centrifuge, wherein the centrifugal speed is 6000-10000 r/min, and the centrifugal time is 15-30 min; centrifuging and filtering to obtain protein supernatant;
(6) vacuum freeze drying: carrying out vacuum freeze drying on the protein supernatant under the conditions that the freezing temperature is-30 to-65 ℃, the vacuum degree is 100Pa, the vacuum freeze drying is carried out for 24 hours, and the water content is less than 5 percent; candida parapsilosis protein;
(7) and (3) storage: putting the nostoc proteins into a container, and preserving at the temperature of-20 to-40 ℃.
Preferably, the draining time in step (2) is 3.5h, the drying time is 40min, and the drying temperature is 60 ℃.
Preferably, in step (3), the stirring power is 0.85kw/m3 and the stirring time is 12 min.
Preferably, the homogenization in step (4) is performed 2 times, and each time for 2 minutes.
Preferably, the centrifugal speed in the step (5) is 6500r/min, and the centrifugal time is 16 min.
Preferably, the freezing temperature in step (6) is-50 ℃.
Preferably, the temperature in step (7) is-20 ℃.
The beneficial effect of the invention is that,
1. simplifies the operation difficulty, overcomes the harsh conditions, has far better extraction rate than the prior art, and can extract about 70 percent of protein in the nostoc.
2. The process has the advantages of mild conditions, no phase change, low energy consumption, no need of introducing inorganic salt ions, simple and convenient operation, short time consumption, easy storage and large-scale production.
3. The protein obtained by adopting the freeze drying method is easier to be actually stored, and a convenient, quick and easy-to-store method is provided for industrially applying the nostoc proteins;
4. homogenizing for multiple times to break cell wall of Nostoc, adding purified water as solvent to extract Nostoc protein, increasing protein extraction efficiency, and greatly improving the retention of Nostoc holoprotein.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The invention is further illustrated by the following examples:
example 1:
an extraction process of nostoc proteins comprises the following steps:
(1) pretreatment: taking 3 parts of nostoc, and putting the nostoc into water to clean and remove impurities for later use;
(2) drying and grinding: draining the pretreated nostoc for 3.5h, and drying in a dryer for 40min at 60 deg.C; grinding and crushing the dried nostoc and sieving the crushed nostoc by a 70-mesh sieve; grinding, adding purified water, and mixing at a ratio of 1: 30;
(3) stirring: putting the mixed solution into a stirrer, and stirring at room temperature for 12min, wherein the stirring power is 0.85kw/m 3;
(4) homogenizing: placing the mixed solution into a homogenizer, homogenizing for 2 times under 30MPa, wherein the time for homogenizing is 2 minutes each time, and obtaining a homogenized solution;
(5) centrifuging: putting the homogenized solution into a centrifuge, and centrifuging at 6500r/min for 16 min; centrifuging and filtering to obtain protein supernatant;
(6) vacuum freeze drying: carrying out vacuum freeze drying on the protein supernatant under the conditions of freezing temperature of-50 ℃, vacuum degree of 100Pa, vacuum freeze drying for 24h and water content of less than 5%; candida parapsilosis protein;
(7) and (3) storage: putting the nostoc proteins into a container, and preserving at the temperature of minus 20 ℃.
Example 2:
an extraction process of nostoc proteins comprises the following steps:
(1) pretreatment: taking 3 parts of nostoc, and putting the nostoc into water to clean and remove impurities for later use;
(2) drying and grinding: draining the pretreated nostoc for 3h, and drying in a dryer for 30min at 55 ℃; grinding and crushing the dried nostoc and sieving the crushed nostoc by a 70-mesh sieve; grinding, adding purified water, and mixing at a ratio of 1: 30;
(3) stirring: putting the mixed solution into a stirrer, and stirring at room temperature for 10min, wherein the stirring power is 0.70kw/m 3;
(4) homogenizing: placing the mixed solution into a homogenizer, homogenizing for 1 time under 30MPa, wherein the homogenizing time is 2 minutes each time, and obtaining a homogenized solution;
(5) centrifuging: placing the homogeneous solution in a centrifuge at 6000r/min for 15 min; centrifuging and filtering to obtain protein supernatant;
(6) vacuum freeze drying: carrying out vacuum freeze drying on the protein supernatant under the conditions of freezing temperature of-30 ℃, vacuum degree of 100Pa, vacuum freeze drying for 24h and water content of less than 5%; candida parapsilosis protein;
(7) and (3) storage: putting the nostoc proteins into a container, and preserving at the temperature of minus 20 ℃.
Of course, the above description is not limited to the above examples, and the undescribed technical features of the present invention can be implemented by or using the prior art, and will not be described herein again; the above embodiments are merely for illustrating the technical solutions of the present invention and not for limiting the present invention, and the present invention has been described in detail with reference to the preferred embodiments, and those skilled in the art should understand that changes, modifications, additions or substitutions which are made by those skilled in the art within the spirit of the present invention are also within the scope of the claims of the present invention.
Claims (6)
1. The extraction process of the nostoc proteins is characterized by comprising the following steps of:
(1) pretreatment: taking 3 parts of nostoc, and putting the nostoc into water to clean and remove impurities for later use;
(2) drying and grinding: draining the pretreated nostoc for 3-4 h, and drying in a dryer at the temperature of 55-70 ℃ for 30-50 min; grinding and crushing the dried nostoc and sieving the crushed nostoc by a 70-mesh sieve; grinding, adding purified water, and mixing at a ratio of 1: 30;
(3) stirring: putting the mixed solution into a stirrer, and stirring at room temperature, wherein the stirring power is 0.70-0.90 kw/m3, and the stirring time is 10-20 min;
(4) homogenizing: placing the mixed solution into a homogenizer, homogenizing for 1-3 times under 30MPa, wherein the time for homogenizing for each time is 2-4 minutes, and obtaining a homogenized solution;
(5) centrifuging: putting the homogeneous liquid into a centrifugal machine, wherein the centrifugal speed is 6000-10000 r/min, and the centrifugal time is 15-30 min; centrifuging and filtering to obtain protein supernatant;
(6) vacuum freeze drying: carrying out vacuum freeze drying on the protein supernatant under the conditions that the freezing temperature is-30 to-65 ℃, the vacuum degree is 100Pa, the vacuum freeze drying is carried out for 24 hours, and the water content is less than 5 percent; candida parapsilosis protein;
(7) and (3) storage: putting the nostoc proteins into a container, and preserving at the temperature of-20 to-40 ℃.
2. The extraction process of nostoc proteins according to claim 1, wherein the draining time in step (2) is 3.5h, the drying time is 40min, and the drying temperature is 60 ℃.
3. The extraction process of nostoc proteins according to claim 1, wherein the stirring power in step (3) is 0.85kw/m3 and the stirring time is 12 min.
4. The extraction process of nostoc proteins as claimed in claim 1, wherein the homogenization in step (4) is performed 2 times, each time for 2 minutes.
5. The extraction process of nostoc proteins according to claim 1, wherein the centrifugation speed in step (5) is 6500r/min and the centrifugation time is 16 min.
6. The extraction process of nostoc proteins according to claim 1, wherein the freezing temperature in step (6) is-50 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210235699.4A CN114539349A (en) | 2022-03-11 | 2022-03-11 | Extraction process of nostoc proteins |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210235699.4A CN114539349A (en) | 2022-03-11 | 2022-03-11 | Extraction process of nostoc proteins |
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| CN114539349A true CN114539349A (en) | 2022-05-27 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304168A (en) * | 2011-08-17 | 2012-01-04 | 华南理工大学 | Low-temperature ultrahigh-pressure preparation method of chlorella protein |
| CN103653122A (en) * | 2013-11-26 | 2014-03-26 | 刘芳圃 | Dandelion and Nostoc commune compound protein beverage process and preparation method thereof |
| CN107488695A (en) * | 2017-08-25 | 2017-12-19 | 兰溪市哥特生物技术有限公司 | A kind of preparation method of the nostoc albumen of promotion plant growth |
| CN108794565A (en) * | 2018-04-27 | 2018-11-13 | 胡梦丽 | A kind of preparation method of spirulina protein powder |
-
2022
- 2022-03-11 CN CN202210235699.4A patent/CN114539349A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304168A (en) * | 2011-08-17 | 2012-01-04 | 华南理工大学 | Low-temperature ultrahigh-pressure preparation method of chlorella protein |
| CN103653122A (en) * | 2013-11-26 | 2014-03-26 | 刘芳圃 | Dandelion and Nostoc commune compound protein beverage process and preparation method thereof |
| CN107488695A (en) * | 2017-08-25 | 2017-12-19 | 兰溪市哥特生物技术有限公司 | A kind of preparation method of the nostoc albumen of promotion plant growth |
| CN108794565A (en) * | 2018-04-27 | 2018-11-13 | 胡梦丽 | A kind of preparation method of spirulina protein powder |
Non-Patent Citations (2)
| Title |
|---|
| 丁文杰: "发状念珠藻不同细胞破碎方法的研究", 《现代食品科技》, vol. 25, no. 11, pages 1243 - 1245 * |
| 梁文裕: "普通念珠藻（ Nostoc commune Vauch．）藻蓝蛋白的提取", 《安徽农业科学》, vol. 36, no. 1, pages 1 * |
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