CN102304168A - Low-temperature ultrahigh-pressure preparation method of chlorella protein - Google Patents
Low-temperature ultrahigh-pressure preparation method of chlorella protein Download PDFInfo
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- CN102304168A CN102304168A CN201110234941A CN201110234941A CN102304168A CN 102304168 A CN102304168 A CN 102304168A CN 201110234941 A CN201110234941 A CN 201110234941A CN 201110234941 A CN201110234941 A CN 201110234941A CN 102304168 A CN102304168 A CN 102304168A
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Abstract
The invention provides a low-temperature ultrahigh-pressure preparation method of chlorella protein, which comprises: mixing chlorella power and pure water in a mass ratio of 1:10 to 1:30, and stirring for 30 to 90 minutes to obtain mixed solution; extracting chlorella protein from the mixed solution at 4 to 8 DEG C and under 100 to 200 MPa to obtain coarse extract solution; and subjecting the coarse extract solution to centrifugal separation, concentrating and drying supernate, and obtaining chlorella protein powder. In the invention, the application of the ultrahigh pressure makes the walls of the chlorella broken completely, the need of heating is obviated, and low-temperature operation is favorable for protecting the bioactivity of protein; the need of adding enzyme is obviated, and cost is expected to be reduced; and acid and alkali are not added, so pollution of acid and alkali is avoided. The method is simple in preparation process, quick and suitable for large-batch production, and is a safe and high-efficiency method for industrial preparation of chlorella protein.
Description
Technical field
The present invention relates to bio-pharmaceuticals engineering and the proteinic preparation of healthy food material chlorella, particularly relate to the proteinic low-temperature ultrahigh-pressure preparation method of chlorella.
Background technology
Chlorella is that one-celled plants can breed fast and grow is uniquely in the plant on the earth can increase by 4 times species at 20 h.Chlorella is of high nutritive value to have and presses down that knurl, anticancer, enhancing immunity, anti-infection ability, detoxifcation protect the liver, the protein content of its dry powder of hypotensive effect is about 50% and quality is also fine so chlorella is a kind of source of good protein.
Yet since chlorella have more complete cell wall structure therefore its intracellular protein extraction relatively difficulty generally all need carry out broken wall treatment and could effectively extract.At present traditional chlorella protein extracting method adopts still mostly that to extract the little energy consumption of extraction yield low processing capacity behind multigelation, ultrasonic, enzyme process, acid system and the alkaline process broken wall high.Particularly to use high temperature (100 ℃) protein active to be destroyed easily usually.
Summary of the invention
Being defective and the deficiency that solves above-mentioned correlation technique, is the mass-producing development and use of chlorella albumen at bio-pharmaceuticals engineering and protective foods production field simultaneously, and the present invention provides chlorella proteinic low-temperature ultrahigh-pressure preparation method.
For realizing the object of the invention, adopt following technical scheme:
(1) with after chlorella powder and the mass ratio mixing of pure water, stirs and got mixing solutions in 30 minutes～90 minutes with 1:10～1:30;
(2) be that 4 ℃～8 ℃, pressure are to extract chlorella protein under the condition of 100MPa～200MPa with above-mentioned mixing solutions in temperature, obtain crude extract;
(3) above-mentioned crude extract is carried out centrifugation, with the supernatant liquor after separating concentrate, drying, obtain the chlorella protein powder.
As preferably, said step (1) chlorella powder and pure water mix with the mass ratio of 1:15～1:25.
As preferably, said step (2) pressure is 120MPa～180MPa.
For further realizing the object of the invention, the solution of collecting after said step (2) is extracted extracts as solution to be extracted next time again, and the number of times of extraction is 1 time～5 times, and each time of extracting is 10min～30min.
Above operation steps if no special instructions, all by this area routine operation.
Compared with prior art the present invention has following advantage:
(1) the present invention uses ultra-high voltage 100MPa～200MPa to extract chlorella albumen, and albumen mass-energy snap-out release comes out in the thorough back of broken wall to make chlorella;
(2) the present invention uses low temperature to extract chlorella albumen for 4 ℃～8 ℃, helps protecting the proteic biological activity of chlorella;
(3) but simple, the consuming time weak point preparation of industrialization of preparing method's technology of the present invention.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail, but embodiment to should not be construed as be the qualification to protection domain of the present invention.
Embodiment 1
(1) chlorella powder and pure water are mixed by the mass ratio of 1:15 and stirred 60 minutes, mixing solutions;
(2) the gained mixing solutions is sent into low-temperature ultrahigh-pressure continuous flow cell crusher and is extracted chlorella protein,
Controlled temperature is that 5 ℃, pressure are 180MPa, extracts the solution collected as the solution to be extracted that extracts next time, repeats to extract operation 3 times, and each extraction time is 10min, obtains extract;
(3) with above-mentioned crude extract with the speed of 10000 r/min in 4 ℃ of centrifugal 20min down, with 30kDa ultra-filtration membrane ultrafiltration and concentration, the filtrating after concentrated obtains the chlorella protein powder-20 ℃ of lyophilizes with the supernatant liquor that obtains after separating.
Embodiment 2
(1) chlorella powder and pure water are mixed by the mass ratio of 1:25 and stirred 70 minutes, mixing solutions;
(2) the gained mixing solutions is sent into low-temperature ultrahigh-pressure continuous flow cell crusher and extracted chlorella protein, controlled temperature is that 4 ℃, pressure are 140MPa, and extraction time is 30min, obtains extract;
(3) with above-mentioned crude extract with the speed of 10000 r/min in 4 ℃ of centrifugal 30min down, the supernatant liquor that obtains after centrifugal is used 20kDa ultra-filtration membrane ultrafiltration and concentration again, the filtrating after concentrating obtains the chlorella protein powder-20 ℃ of lyophilizes.
Embodiment 3
(1) chlorella powder and pure water are mixed by the mass ratio of 1:30 and stirred 90 minutes, mixing solutions;
(2) the gained mixing solutions is sent into low-temperature ultrahigh-pressure continuous flow cell crusher and is extracted chlorella protein; Controlled temperature is that 8 ℃, pressure are that 150MPa will extract the solution collected as the solution to be extracted that extracts operation next time; Repeat to extract operation 4 times; Each extraction time is 25min, obtains crude extract;
(3) in 4 ℃ of centrifugal 20min down, the supernatant liquor that obtains after centrifugal is used 40kDa ultra-filtration membrane ultrafiltration and concentration to above-mentioned crude extract again with the speed of 12000 r/min, and the filtrating after concentrating obtains the chlorella protein powder-20 ℃ of lyophilizes.
Embodiment 4
(1) chlorella powder and pure water are mixed by the mass ratio of 1:25 and stirred 70 minutes, mixing solutions;
(2) the gained mixing solutions is sent into low-temperature ultrahigh-pressure continuous flow cell crusher and extracted chlorella protein; Controlled temperature is that 7 ℃, pressure are 130MPa; The to be extracted solution of the solution of collection as extraction operation next time will be extracted; Repeat to extract operation 5 times; Each extraction time is 15min, obtains crude extract;
(3) with the centrifugal back of above-mentioned crude extract with the speed of 12000 r/min in 8 ℃ of centrifugal 20min down, the supernatant liquor that obtains after centrifugal is used 50kDa ultra-filtration membrane ultrafiltration and concentration again, the filtrating after concentrating obtains the chlorella protein powder-20 ℃ of lyophilizes.
Claims (4)
1. the proteinic low-temperature ultrahigh-pressure preparation method of chlorella is characterized in that extraction step is following:
(1) got mixing solutions in 30 minutes～90 minutes with stirring after chlorella powder and the mass ratio mixing of pure water with 1:10～1:30;
(2) be that 4 ℃～8 ℃, pressure are to extract chlorella protein under the condition of 100 MPa～200MPa to obtain crude extract with above-mentioned mixing solutions in temperature;
(3) above-mentioned crude extract is carried out centrifugation, with the supernatant liquor after separating concentrate, drying obtains the chlorella protein powder.
2. low-temperature ultrahigh-pressure preparation method according to claim 1 is characterized in that said step (1) chlorella powder and pure water mix with the mass ratio of 1:15～1:25.
3. low-temperature ultrahigh-pressure preparation method according to claim 1 and 2 is characterized in that said step (2) pressure is 120 MPa～180 MPa.
4. low-temperature ultrahigh-pressure preparation method according to claim 1; It is characterized in that the solution conduct solution of collecting after said step (2) is extracted to be extracted next time extracts again; The number of times that extracts is 1 time～5 times, and each time of extracting is 10min～30min.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851345A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella antitumor polypeptide |
| CN102851343A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella antitumor polypeptide |
| CN102847134A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella polypeptide microcapsule |
| CN102846553A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella polypeptide-chitosan nanoparticle |
| WO2015007997A1 (en) * | 2013-07-19 | 2015-01-22 | Roquette Freres | Optimised method for breaking chlorella cell walls by means of very high pressure homogenisation |
| CN104688779A (en) * | 2013-12-09 | 2015-06-10 | 舒梅 | An ultra-high pressure extraction method of silkworm chrysalises |
| CN104973275A (en) * | 2014-04-14 | 2015-10-14 | 东营大振生物科技有限公司 | Chlorella split charging device |
| CN106398857A (en) * | 2016-11-29 | 2017-02-15 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Method for extracting Schizochytrium limacinum oil and application thereof |
| US10465159B2 (en) | 2013-07-04 | 2019-11-05 | Corbion Biotech, Inc. | Optimised method for breaking chlorella walls by mechanical crushing |
| CN114539349A (en) * | 2022-03-11 | 2022-05-27 | 青岛浩然海洋科技有限公司 | Extraction process of nostoc proteins |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367257A (en) * | 2002-02-08 | 2002-09-04 | 江苏省农业科学院土壤肥料研究所 | Extraction method of chlorella growth factor (chlorella extract and CGF) |
| CN101978974A (en) * | 2010-10-20 | 2011-02-23 | 汤永强 | Method for extracting bioactive substance by breaking walls of chlorella pyrenoidosa chick |
-
2011
- 2011-08-17 CN CN201110234941A patent/CN102304168A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367257A (en) * | 2002-02-08 | 2002-09-04 | 江苏省农业科学院土壤肥料研究所 | Extraction method of chlorella growth factor (chlorella extract and CGF) |
| CN101978974A (en) * | 2010-10-20 | 2011-02-23 | 汤永强 | Method for extracting bioactive substance by breaking walls of chlorella pyrenoidosa chick |
Non-Patent Citations (4)
| Title |
|---|
| 丁文杰: "发状念珠藻不同细胞破碎方法的研究", 《现代食品科技》, vol. 25, no. 11, 31 December 2009 (2009-12-31) * |
| 刘立闯: "螺旋藻蛋白提取方法比较研究", 《食品科学》, vol. 29, no. 11, 31 December 2008 (2008-12-31) * |
| 胡蓓娟等: "从微藻中分离提取生物活性物质", 《食品科学》, vol. 27, no. 7, 31 December 2006 (2006-12-31) * |
| 陈岩: "小球藻的高压诱变及生理生化响应", 《中国优秀硕博士学位论文全文数据库(硕士) 基础科学辑》, no. 5, 15 May 2007 (2007-05-15), pages 006 - 102 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851345A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella antitumor polypeptide |
| CN102851343A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella antitumor polypeptide |
| CN102847134A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella polypeptide microcapsule |
| CN102846553A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella polypeptide-chitosan nanoparticle |
| CN102846553B (en) * | 2012-08-31 | 2014-04-02 | 华南理工大学 | Preparation method for chlorella polypeptide-chitosan nanoparticle |
| CN102847134B (en) * | 2012-08-31 | 2014-07-02 | 华南理工大学 | Preparation method for chlorella polypeptide microcapsule |
| CN102851345B (en) * | 2012-08-31 | 2014-07-30 | 华南理工大学 | Preparation method for chlorella antitumor polypeptide |
| US10465159B2 (en) | 2013-07-04 | 2019-11-05 | Corbion Biotech, Inc. | Optimised method for breaking chlorella walls by mechanical crushing |
| FR3008712A1 (en) * | 2013-07-19 | 2015-01-23 | Roquette Freres | OPTIMIZED METHOD OF BREAKING CHLORELIC WALLS BY HOMOGENIZATION AT VERY HIGH PRESSURE |
| JP2016524908A (en) * | 2013-07-19 | 2016-08-22 | ロケット フレールＲｏｑｕｅｔｔｅ Ｆｒｅｒｅｓ | Optimal destruction method of cell wall of chlorella by ultra high pressure homogenization |
| WO2015007997A1 (en) * | 2013-07-19 | 2015-01-22 | Roquette Freres | Optimised method for breaking chlorella cell walls by means of very high pressure homogenisation |
| CN104688779A (en) * | 2013-12-09 | 2015-06-10 | 舒梅 | An ultra-high pressure extraction method of silkworm chrysalises |
| CN104688779B (en) * | 2013-12-09 | 2019-02-01 | 四川好医生中藏药业有限公司 | A kind of superhigh pressure extracting method of silkworm chrysalis |
| CN104973275A (en) * | 2014-04-14 | 2015-10-14 | 东营大振生物科技有限公司 | Chlorella split charging device |
| CN106398857A (en) * | 2016-11-29 | 2017-02-15 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Method for extracting Schizochytrium limacinum oil and application thereof |
| CN114539349A (en) * | 2022-03-11 | 2022-05-27 | 青岛浩然海洋科技有限公司 | Extraction process of nostoc proteins |
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Application publication date: 20120104 |