CN102302116A - Calcium supplementing and calcium locking health care food and preparation method thereof - Google Patents
Calcium supplementing and calcium locking health care food and preparation method thereof Download PDFInfo
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Abstract
The invention provides a calcium supplementing and calcium locking health care food. The calcium supplementing and calcium locking health care food comprises calcium, colostrum basic protein, chondroitin sulfate, D-glucosamine, vitamin D, casein phosphopeptides and the like, wherein the colostrum basic protein is obtained by degreasing, separating, concentrating, removing casein, alpha-lactoalbumin, beta-lactoglobulin and substances with molecular weights of more than 30 kDa and less than 1 kDa and drying at a low temperature; the biggest difference between the calcium supplementing and calcium locking health care food prepared by the method provided by the invention and the prior art is that the capacity of the bone tissue in actively absorbing osteoblast nutrition like calcium, ossein and the like can be effectively improved and effects of activating osteoblasts and inhibiting osteoclast activity to enhance bone mineral density and length and promote the health of the bone are obtained. The calcium supplementing and calcium locking health care food provided by the invention, which is a latest generation of domestic osteoarthrosis rehabilitation product, obtains breakthrough in the calcium supplementing effect, can be also used for helping the bone to preferably absorb the calcium in the food, has no toxic and side effect after being taken for a long time.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof.Be particularly related to a kind of colostrum basic protein composition that contains, be used to the Ricipe for health care food of lock calcium and preparation method thereof of replenishing the calcium.
Background technology
Because one of nutrition problem that the influence of dietary structure, calcium deficiency are Chinese to be faced has for a long time formed huge market for the product of replenishing the calcium thus.But for many years; The product of replenishing the calcium centers on passive additional various calcium agent basically to be developed; And effective absorptivity problem of various calcium agent also becomes the focus of paying close attention in recent years with the negative effect that excessively additional calcium agent brings; For example: excessive interpolation is calcareous or add calcareously carelessly, and now the replenish the calcium calcium content of product of part surpasses suggestion dose every day of U.S. RDI even surpasses 1000 milligrams high level on the market; Or coveting to replenish the calcium to contain at some adds calcareously carelessly in the oxalic acid food with high, declares high-calcium formula.Long-term edible these high calcium products can combine to generate calcium oxalate with the oxalic acid in the food and deposit in vivo, form renal lesionses such as kidney stone, ephritis even chronic renal failure.
Outer state-ownedly discover that much skeleton bulking disease patient surpasses five to ten years as taking anti-bone-loss medicine such as bis phosphoric acid salt medicine, just may cause the atypia fracture.The researcher studies this sick pathology, finds that after microscopic examination the firm degree of atypia fracture patient sclerotin is as good as with old bone.Originally; Anti-bone-loss medicine can reduce bone-loss; Only suppress broken bone function, have no idea to make new bone, the long-term use down; In a disguised form with in the residual body of old bone; Be that old bone originally needed to run off through metabolism, but medicine keeps old bone, even so that the interior sclerotin density of patient's body high again; But bone is still unbearably fragile, and risk of bone fracture goes up not down.Make a general survey of the bone health care course in the whole world, roughly can be divided into three phases: the phase I is to replenish the calcium merely; Second stage is to replenish the calcium to add to replenish the function factor stage that promotes that calcium absorbs, and as adding the absorption that vitamin D promotes calcium, the phase III is the comprehensive reparation and the regeneration phase of bone.Nearly all strong bone product all is in second stage at present.
Calcareous and bone collagen all is a main material of building bone, and calcareous meeting is attached to the firm bone of formation on the bone collagen.The gelatine proper energy helps to absorb calcareous, defends calcium loss simultaneously, and human body need absorb enough calcareous and bone collagens and make bone.In people's children and adolescence, osteoblast is more active than osteoclast, belongs to the skeletal growth phase, and the people constantly grows tall; After entering was grown up, osteoblast and osteoclast kept balance, and bone stops growing; And after getting into old age, osteoclast is more active than osteoblast, causes a large amount of calcium loss, thereby forms osteoporosis.At microscopically, the bone of osteoporosis appears, and the obviously normal bone of its bone trabecula is few, and it is big that hole becomes, and the density of bone descends, and it is fragile that sclerotin becomes.
Summary of the invention
The defective of body malabsorption in order to overcome the unilateral accumulation calcium in human body of the existing product of replenishing the calcium; The invention provides a kind of contain active ingredients such as colostrum basic protein, calcium, chondroitin sulfate, D-Glucosamine, CPP replenish the calcium, lock calcium health food and preparation method thereof; Product of the present invention can effectively improve the ability that bone tissue active absorption calcium and ossein etc. are made osteotrophy, has the health care of replenishing the calcium, consolidate calcium, lock calcium.
It is of the present invention that to replenish the calcium, lock the calcium health food be formulated by weight percentage by following composition:
Calcium 10-35% colostrum basic protein 1-5%
Chondroitin sulfate 2-10% D-Glucosamine 10-40%
Vitamin D 0.05-0.5% CPP 2-8%
The pregelatinized starch surplus.
Of the present inventionly replenish the calcium, lock the calcium health food and fill a prescription preferably and be:
Calcium 25-35% colostrum basic protein 2-5%
Chondroitin sulfate 5-10% D-Glucosamine 20-40%
Vitamin D 0.1-0.3% CPP 5-8%
The pregelatinized starch surplus.
Calcium described in the prescription can be one or more of substantial equivalence compositions such as calcium lactate, milk calcium, calcium pantothenate, calcium carbonate, calcium citrate, calcium acetate, amino acid chelated calcium, calcium gluconae, citric acid and calcium malate; Wherein, Calcium lactate is white particle or powder; Free from extraneous odour; Little have a weathering property; Be soluble in hot water, have solubility height, dissolution velocity is fast, bioavailability is high, mouthfeel is good advantage; Chondroitin sulfate is the acid mucopolysaccharide of extraction, purifying gained from the animal cartilaginous tissue; It is a kind of macromolecular compound; Can strengthen cellular immune function, humoral immune function and non-specific immune function; Growth that simultaneously can the induced osteogenesis cell; Be adjusted to the osteocyte calcification; Promote the calcium deposition, have enhance immunity power and the effect that increases bone density; D-Glucosamine of the present invention can be the D-aminoglucose hydrochloride, also can be the D-aminoglucose sulfate, and the D-Glucosamine is the composition of poly glucosamine in cartilage matrix and the synovia.Humans and animals is synthesizing amino glucose in vivo, but along with the increase at age, the speed that biosynthetic speed is unable to catch up with degrading will lack Glucosamine in the body, and then influences cartilage cell's metabolism.Glucosamine all has tangible humidification to body specific cellular immunity function, humoral immune function and non-specific immune function; Glucosamine can be active through promoting Gegenbaur's cell simultaneously; Increase the expression of bone morphogenetic protein; Promote trace elements absorbed such as calcium, phosphorus; Increase the fixation of bone tissue to calcium; Avoid calcium loss, thereby effectively increase bone density.Exogenous Glucosamine specific effect is in articular cartilage; Can stimulate cartilage cell's synthetic proteins polysaccharide, replenish the missing element of cartilage matrix, recover cartilage cell's eubolism function; Enable the proteoglycan of synthetic normal configuration, safeguard the morphosis of cartilage matrix; Vitamin D mainly contains following physiological function: improve the absorption of human body to calcium, phosphorus; Make the level of plasma calcium and the blood plasma phosphorus degree that reaches capacity; Promote growth and bone calcification; Promote tooth sound; Increase the absorption of phosphorus through the intestines wall; And pass through the absorption again that renal tubule increases phosphorus, keep the normal level of citrate in the blood; CPP can promote small intestine to the absorption of calcium, promote the utilization of bone and tooth to calcium; Colostrum basic protein among the present invention is from normal raising; The healthy cow of non-communicable disease and mammitis divides the milk of being extruded in 72 hours puerperiums to separate through degreasing; Concentrate; Remove casein; α-lactoalbumin; β-lactoglobulin matter and molecular wt more than 30 kDa with material below 1 kDa; Through frozen drying or spray-dried after; Refine the low-molecular-weight activated protein that forms through ultrafiltration; Colostrum is used above 50 years as a kind of nutrition treasure; Colostrum not only protein content is high; And contain the rare albumen that does not have in many other dairy products; The present invention adds the colostrum basic protein in Ricipe for health care food; With chondroitin sulfate; CPP; Synergies such as vitamin D; Can make it directly act on bone cells; It is movable to suppress osteoclast; Promote osteoblast quantity to increase and liveness; And the activity of coordination Gegenbaur's cell and osteoclast; The dynamic equilibrium that keeps both; Promote bone metabolism, and then reach the promotion bone growth; Repair the purpose of sclerotin.
Calcium lactate of the present invention is the auspicious general board calcium lactate of Zhengzhou Ruipu Bioengineering Co., Ltd.; Vitamin D is the Zhejiang new synthetic board vitamin D of synthetic limited company newly; Glucosamine is the fengrun board D-aminoglucose hydrochloride of Taizhou City Fengrun Biochemical Co., Ltd; Chondroitin sulfate is the extra large treasure board chondroitin sulfate of sea, Shandong treasure Bioisystech Co., Ltd; CPP is the cosen board CPP that Jiangxi can biochemical Co., Ltd; Pregelatinized starch is the merely medicine board pregelatinized starch of Ahua Pharmaceutical Co., Ltd., Liaocheng, Shandong.
The preparation method who replenishes the calcium, locks the calcium health food of the present invention is following:
1) liquid bovine colostrum is adopted centrifugation 20-40 minute under the condition of 2～5 ℃ of temperature, rotating speed 3000-5000 rev/min of cryogenic freezing centrifuge, removes the upper strata fat deposit, fat content less than 0.08% degreasing liquid bovine colostrum.Temperature is too high to be unfavorable for centrifugally, and destroys the immune globulin activity factor in Ruzhong just easily, so temperature is controlled at below 5 ℃ centrifugal effective can't exerting an influence to immunoglobulin (Ig).Afterwards, adopt flash pasteurization to sterilize to the colostrum after the degreasing, sterilising temp 60-63 ℃, sterilization time 10-20 second;
2) to be adjusted to the pH value be 4.6 to the liquid bovine colostrum after using 1 N hydrochloric acid with degreasing, sterilization; Make casein from colostrum, solidify deposition; In industrial centrifuge be with the rotating speed 3000-5000 rev/min the condition casein that will solidify through 30-50 minute centrifugal treating, thereby separate with lactalbumin, nonprotein, lactose, mineral matter etc. in the solution.It is that product contains and the original identical colloid calcium phosphate of level that centrifugal process is separated caseic advantage, can in water, the form with micella disperse, its character also with original micella similar performance;
3) pH value to 3.9 removal α-lactoalbumin: with the trisodium citric acid set-up procedure 2 of 1.5M); And be heated to 30-35 ℃ and reach 120-150 minute; Treat the α-lactoalbumin of α-lactoalbumin post precipitation, centrifugal rotational speed 3000-5000 rev/min through centrifugal filtration removal deposition;
4) remove β-lactoglobulin matter: the acid-base value of step 3) is adjusted to pH4.5 with the 1N sodium hydroxide solution; Made the beta lactoglobulin polymerization be able to remove in 30 minutes with 18000 rev/mins speed centrifugal filtration then, this step also can be removed the casein of remainder;
5) ultrafiltration: with the ultrafiltration membrance filter of 100 kDa and 30 kDa, remove the material of molecular wt unit successively continuously greater than 100 kDa and 30 kDa.Again with milipore filter with part molecular wt unit other scintillas less than 1 kDa; Like salt and lactose removal; Promptly getting molecular wt is the material of 1 kDa～30 kDa, carries out ultrafiltration with ultrafiltration membrane, and the range of structures in the hole of milipore filter is 0.001-0.02 μ m; Can be with macromolecular substances such as protein; Fat globule, polysaccharide etc. are held back, part lactose and moisture; Through film, operating pressure is generally 0.3-1.0 Mpa to ash content as seeing through thing;
6) adopt existing hypothermic lyophilization technology or spray drying technology that step 5) is carried out drying; With for use behind the dried colostrum basic protein particle grinding; Should concentrate colostrum basic protein Stability Analysis of Structures, biologically active does not have to change basically, and protein content is greater than 80%.
7) calcium, chondroitin sulfate, Glucosamine, vitamin D, CPP, pregelatinized starch are crossed 80 mesh sieves respectively, subsequent use;
8) chondroitin sulfate, the CPP with formula rate mixes in three-dimensional mixer; The mode of progressively increasing by equivalent adds calcium, Glucosamine, the pregelatinized starch of formula rate successively again, fully mixes, and gets mixed powder; This mixed powder is put in the trough type mixing machine; The adding percentage by weight is that 20-40%, concentration are 50% ethanolic solution mixing; Make softwood; Softwood is put into oscillating granulator 16-30 mesh sieve granulates; Get wet granular; And with wet granular 50-60 ℃ dry 2-4 hour down, cross the 16-25 mesh sieve, must do particle;
9) vitamin D of formula rate and the colostrum basic protein fraction powder of equivalent are mixed in three-dimensional mixer; The mode that increases progressively by equivalent successively adds remaining colostrum basic protein fraction powder and dried particle again; Fully mix; Get total mixture; For avoiding the change of vitamin D and colostrum basic protein character in granulation, dry run, so add in the in the end always mixed operation of taking to add of mode;
10) above-mentioned total mixture is processed granule, tablet or capsule according to the market demand by prior art, packing promptly gets product of the present invention.
Of the present inventionly replenish the calcium, lock the calcium health food and maximum not being both of current other bone health product can effectively improve the ability that bone tissue active absorption calcium and ossein etc. are made osteotrophy; But not simple supplementary calcium food, thereby the adding of colostrum basic protein can make product of the present invention have the activation osteoblast and suppress the osteoclast activity, makes calcareous quilt effectively absorb, reduce loss enhance bone density and length, the effect of promotion bone health; Calcium supplement health care food of the present invention has not only been obtained breakthrough replenishing the calcium to render a service, and can also help bone calcareous in assimilating food better, and long-term ediblely have no toxic and side effect, is the latest domestic generation osteoarthropathy good merchantable brand of recuperating.
The specific embodiment
Embodiment 1:
This enforcement is described to be used to replenish the calcium, the health food of solid calcium, lock calcium is made up of following ingredients by weight percentage:
Calcium lactate 10 kg calcium citrate 15kg
Milk calcium 10kg colostrum basic protein 4 kg
Chondroitin sulfate 10 kg D-aminoglucose sulfates 20 kg
Vitamin D (0.25%) 0.2 kg CPP 8 kg
Pregelatinized starch 22.8 kg
The preparation process is following:
1) liquid bovine colostrum is adopted the cryogenic freezing centrifuge under the condition of 2～5 ℃ of temperature, 4000 rev/mins of rotating speeds, centrifugalize 30 minutes, removes the upper strata fat deposit, fat content less than 0.08% degreasing liquid bovine colostrum.Afterwards, adopt flash pasteurization to sterilize to the colostrum after the degreasing, sterilising temp 60-63 ℃, sterilization time 15 seconds;
2) to be adjusted to the pH value be 4.6 to the liquid bovine colostrum after using 1 N hydrochloric acid with degreasing, sterilization, makes casein from colostrum, solidify deposition, in industrial centrifuge, is that 4000 rev/mins the condition casein that will solidify was through 40 minutes centrifugal treating with rotating speed;
3) pH value to 3.9 removal α-lactoalbumin: with the trisodium citric acid set-up procedure 2 of 1.5M); And be heated to 30-35 ℃ and reach 120 minutes; Treat the α-lactoalbumin of α-lactoalbumin post precipitation, 4000 rev/mins of centrifugal rotational speeds through centrifugal filtration removal deposition;
4) remove β-lactoglobulin matter: with the 1N sodium hydroxide solution acid-base value of step 3) is adjusted to pH4.5, made the beta lactoglobulin polymerization be able to removal in 30 minutes with 18000 rev/mins speed centrifugal filtration then;
5) ultrafiltration: with the ultrafiltration membrance filter of 100 kDa and 30 kDa, remove the material of molecular wt unit successively continuously greater than 100 kDa and 30 kDa.Again with milipore filter with part molecular wt unit other scintillas less than 1 kDa, like salt and lactose removal, promptly getting molecular wt is the material of 1 kDa～30 kDa.Operating pressure is controlled at the 0.5-0.8Mpa scope;
6) adopt existing hypothermic lyophilization technology that step 5) is carried out drying, and with for use behind the dried colostrum basic protein particle grinding;
7) calcium lactate, milk calcium, calcium citrate, chondroitin sulfate, D-aminoglucose hydrochloride, vitamin D, CPP, pregelatinized starch are crossed 80 mesh sieves respectively, subsequent use;
8) chondroitin sulfate, the CPP with formula rate mixes in three-dimensional mixer; The mode of progressively increasing by equivalent adds calcium lactate, milk calcium, calcium citrate, D-aminoglucose hydrochloride, the pregelatinized starch of formula rate successively again, fully mixes, and gets mixed powder; This mixed powder is put in the trough type mixing machine, added percentage by weight and be 30%, concentration is that 50% ethanolic solution mixes, and makes softwood; Softwood is put into oscillating granulator 16 mesh sieves granulates, wet granular, and with wet granular 50 ℃ dry 4 hours down; Cross 16 mesh sieves, must do particle;
9) vitamin D of formula rate and the colostrum basic protein fraction powder of equivalent are mixed in three-dimensional mixer, the mode that increases progressively by equivalent successively adds remaining colostrum basic protein fraction powder and dried particle again, fully mixes, and gets total mixture;
10) total mixture is put compressing tablet in the rotary tablet machine, selected, reject defective work, packing promptly gets tablet product of the present invention.
Embodiment 2:
The described functional food of replenishing the calcium, consolidate calcium, lock calcium of this enforcement is made up of following ingredients by weight percentage:
Calcium carbonate 25 kg colostrum basic protein 5kg
Chondroitin sulfate 5 kg D-aminoglucose hydrochlorides 40 kg
Vitamin D (0.25%) 0.3 kg CPP 5 kg
Pregelatinized starch 19.7 kg
The preparation process is following:
1) liquid bovine colostrum is adopted the cryogenic freezing centrifuge under the condition of 2～5 ℃ of temperature, 5000 rev/mins of rotating speeds, centrifugalize 20 minutes, removes the upper strata fat deposit, fat content less than 0.08% degreasing liquid bovine colostrum.Afterwards, adopt flash pasteurization to sterilize to the colostrum after the degreasing, sterilising temp 60-63 ℃, sterilization time 20 seconds;
2) to be adjusted to the pH value be 4.6 to the liquid bovine colostrum after using 1 N hydrochloric acid with degreasing, sterilization, makes casein from colostrum, solidify deposition, in industrial centrifuge, is that 5000 rev/mins the condition casein that will solidify was through 30 minutes centrifugal treating with rotating speed;
3) pH value to 3.9 removal α-lactoalbumin: with the trisodium citric acid set-up procedure 2 of 1.5M); And be heated to 30-35 ℃ and reach 150 minutes; Treat the α-lactoalbumin of α-lactoalbumin post precipitation, 3000 rev/mins of centrifugal rotational speeds through centrifugal filtration removal deposition;
4) with embodiment 1;
5) with embodiment 1;
6) adopt existing spray drying technology that step 5) is carried out drying, and with for use behind the dried colostrum basic protein particle grinding;
7) calcium carbonate, chondroitin sulfate, D-aminoglucose hydrochloride, vitamin D, CPP, the pregelatinized starch of formula rate are crossed 80 mesh sieves respectively, subsequent use;
8) chondroitin sulfate, the CPP with formula rate mixes in three-dimensional mixer; The mode of progressively increasing by equivalent adds pregelatinized starch, calcium carbonate, the D-aminoglucose hydrochloride of formula rate successively again, fully mixes, and gets mixed powder; This mixed powder is put in the trough type mixing machine, added percentage by weight and be 20%, concentration is that 50% ethanolic solution mixes, and makes softwood; Softwood is put into oscillating granulator 25 mesh sieves granulates, wet granular, and with wet granular 60 ℃ dry 2 hours down; Cross 20 mesh sieves, must do particle;
9) with embodiment 1;
10) with the total mixture filled capsules of step 9), polish, select the removal defective work, packing promptly gets capsule product of the present invention.
Embodiment 3:
The described functional food of replenishing the calcium, consolidate calcium, lock calcium of this enforcement is made up of following ingredients by weight percentage:
Amino acid chelated calcium 10 kg calcium gluconae 10kg
Calcium acetate 10 kg colostrum basic proteins 2 kg
Chondroitin sulfate 8 kg D-aminoglucose hydrochlorides 30 kg vitamin Ds (0.25%) 0.1 kg CPP 5 kg pregelatinized starch 24.9 kg
The preparation process is following:
1) liquid bovine colostrum is adopted the cryogenic freezing centrifuge under the condition of 2～5 ℃ of temperature, 3000 rev/mins of rotating speeds, centrifugalize 40 minutes, removes the upper strata fat deposit, fat content less than 0.08% degreasing liquid bovine colostrum.Afterwards, adopt flash pasteurization to sterilize to the colostrum after the degreasing, sterilising temp 60-63 ℃, sterilization time 20 seconds.
2) to be adjusted to the pH value be 4.6 to the liquid bovine colostrum after using 1 N hydrochloric acid with degreasing, sterilization, makes casein from colostrum, solidify deposition, in industrial centrifuge, is that 3000 rev/mins the condition casein that will solidify was through 50 minutes centrifugal treating with rotating speed;
3) pH value to 3.9 removal α-lactoalbumin: with the trisodium citric acid set-up procedure 2 of 1.5M); And be heated to 30-35 ℃ and reach 130 minutes; Treat the α-lactoalbumin of α-lactoalbumin post precipitation, 5000 rev/mins of centrifugal rotational speeds through centrifugal filtration removal deposition;
4), 5), 6) all with embodiment 1;
7) amino acid chelated calcium, calcium gluconae, calcium acetate, chondroitin sulfate, D-aminoglucose hydrochloride, vitamin D, CPP, the pregelatinized starch of formula rate are crossed 80 mesh sieves respectively, subsequent use;
8) chondroitin sulfate, the CPP with formula rate mixes in three-dimensional mixer; The mode of progressively increasing by equivalent adds amino acid chelated calcium, calcium gluconae, calcium acetate, pregelatinized starch, the D-aminoglucose hydrochloride of formula rate successively again, fully mixes, and gets mixed powder; This mixed powder is put in the trough type mixing machine, added percentage by weight and be 40%, concentration is that 50% ethanolic solution mixes, and makes softwood; Softwood is put into oscillating granulator 30 mesh sieves granulates, wet granular, and with wet granular 55 ℃ dry 3 hours down; Cross 25 mesh sieves, must do particle;
9) with embodiment 1;
10) with the total mixture packing of step 9), promptly get granule product of the present invention.
The embodiment of the invention 1, embodiment 2, embodiment 3 have been carried out the security toxicological evaluation respectively, and result of the test shows: the test of SD rat acute per os shows that the three is nontoxic level prescription; Salmonella reversion test, mouse marrow cell micro nuclear test and three genetic toxicity tests of mouse sperm deformity test are negative findings; Show that three Example formulations all do not have mutagenesis; In 30 days feeding trials of the rat of 100 times of human body recommended intakes, do not cause the ANOMALOUS VARIATIONS of rat holistic health, biochemical functions and the various indexs of organ-tissue morphology at maximum dose level.
The embodiment of the invention 1, embodiment 2, embodiment 3 have been carried out the animal functional trial respectively, and experimental animal adopts the cleaning level female sd inbred rats of having excised ovary.Three embodiment press the filling gastric juice of 10 times of amounts of human body RD with distilled water preparation respective concentration respectively; As experimental group; Model control group and sham operated rats (not spay group) give isopyknic distilled water respectively; The positive controls per os gives the Allan sodium phosphate of 1.0mg/kgbw; Give animal subject respectively and irritate stomach; Irritate stomach every day once, irritate the long-pending 1.0ml/100gbw of being of body of stomach.The Allan sodium phosphate is the bone metabolism regulators, can suppress osteoclast activity, thereby plays the effect that bone absorbs that suppresses.Be used for the treatment of postmenopausal women's osteoporosis clinically, its main adverse reaction is gastrointestinal reaction such as nausea,vomiting,diarrhea etc.
From on-test, all single cage of each treated animal is raised, and feeds and raises identical feed, irritates stomach and gives the test solution that is subjected to separately, drinks deionized water, the record food-intake.3 totally months.During off-test, rat is put to death in cervical vertebra dislocation, through 105 ℃ of bakings 8 hours, be cooled to room temperature after, with the bone density (BMD) of bone mineral measuring instrument measurement femur midpoint and distal end.After the gross weight of weighing femur, put into triangular flask, add nitric acid+perchloric acid (4:1) mixed acid 15ml, after the digestion, use the deionized water constant volume, use the atomic absorption spectrophotometer calcium content of bone through salt acid treatment dry cleansing.
Result of the test shows: each femur length difference of organizing rat does not have conspicuousness (P>0.05); Sham operated rats rat femur distal end bone density, femur center bone density are significantly higher than oophorectomize+group of solvents (P < 0.05) (model control group), show the modeling success; Three embodiment group femur center bone densities, femur distal end bone density, femur dry weight, femur calcium contents all are significantly higher than model control group (P < 0.05), and the result sees table 1.The result shows that three embodiment groups all have preferably and replenishes the calcium, bone calcium, increases the function of rat bone density.
The influence of table 1 pair rat femur length and bone density
Claims (10)
1. replenish the calcium, lock the calcium health food for one kind, formulated by weight percentage by following composition:
Calcium 10-35% colostrum basic protein 1-5%
Chondroitin sulfate 2-10% D-Glucosamine 10-40%
Vitamin D 0.05-0.5% CPP 2-8%
The pregelatinized starch surplus.
2. as claimed in claim 1ly replenish the calcium, lock the calcium health food, formulated by weight percentage by following composition:
Calcium 25-35% colostrum basic protein 2-5%
Chondroitin sulfate 5-10% D-Glucosamine 20-40%
Vitamin D 0.1-0.3% CPP 5-8%
The pregelatinized starch surplus.
3. as claimed in claim 1 or 2ly replenish the calcium, lock the calcium health food, it is characterized in that described calcium is selected from one or more of calcium lactate, milk calcium, calcium pantothenate, calcium carbonate, calcium citrate, calcium acetate, amino acid chelated calcium, calcium gluconae, citric acid and calcium malate.
4. as claimed in claim 1 or 2ly replenish the calcium, lock the calcium health food, it is characterized in that described D-Glucosamine is D-aminoglucose hydrochloride or D-aminoglucose sulfate.
5. as claimed in claim 1 or 2ly replenishing the calcium, lock the calcium health food, it is characterized in that the colostrum basic protein, is the 1-30kDa low-molecular-weight activated protein that from the colostrum whey, refines.
6. the preparation method who replenishes the calcium, locks the calcium health food as claimed in claim 1 or 2 comprises the steps: that the preparation method who replenishes the calcium, locks the calcium health food of the present invention is following:
1. liquid bovine colostrum being adopted the cryogenic freezing centrifuge, is that 2～5 ℃, rotating speed are centrifugation 20-40 minute under 3000-5000 rev/min the condition in temperature, afterwards, the colostrum after the degreasing is sterilized;
2. it is 4.6 that the liquid bovine colostrum after using 1 N hydrochloric acid with degreasing, sterilization is adjusted to the pH value, and the casein that when rotating speed is 3000-5000 rev/min, will solidify was through 30-50 minute centrifugal treating;
3. with the trisodium citric acid set-up procedure pH value to 3.9 2. of 1.5M, and be heated to 30-35 ℃ and reach 120-150 minute, treat that α-lactoalbumin post precipitation removes the α-lactoalbumin of deposition through centrifugal filtration, centrifugal rotational speed is 3000-5000 rev/min;
4. with the 1N sodium hydroxide solution step acid-base value is 3. adjusted to pH4.5, removed beta lactoglobulin in 30 minutes with 18000 rev/mins speed centrifugal filtration then;
5. continuous ultrafiltration membrance filter with 100 kDa and 30 kDa; Remove the material of molecular wt unit successively greater than 100 kDa and 30 kDa; With milipore filter part molecular wt unit is removed less than other scintillas of 1 kDa, obtaining molecular wt is the material of 1 kDa～30 kDa again;
6. adopt hypothermic lyophilization technology or spray drying technology that 5. step is carried out drying, with for use behind the dried colostrum basic protein particle grinding;
7. calcium, chondroitin sulfate, Glucosamine, vitamin D, CPP, pregelatinized starch are crossed 80 mesh sieves respectively, subsequent use;
8. chondroitin sulfate, the CPP with formula rate mixes in three-dimensional mixer; The mode of progressively increasing by equivalent adds calcium, Glucosamine, the pregelatinized starch of formula rate successively again, fully mixes, and gets mixed powder; This mixed powder is put in the trough type mixing machine, and the adding percentage by weight is that 20-40%, concentration are that 50% ethanolic solution mixes, and makes softwood, softwood is put into oscillating granulator processes wet granular, and wet granular is dry, sieve, must do particle;
9. the vitamin D of formula rate and the colostrum basic protein fraction powder of equivalent are mixed in three-dimensional mixer, the mode that increases progressively by equivalent successively adds remaining colostrum basic protein fraction powder and dried particle again, fully mixes, and gets total mixture;
10. above-mentioned total mixture is processed health food of the present invention.
7. the preparation method who replenishes the calcium, locks the calcium health food as claimed in claim 6 is characterized in that the temperature the when colostrum after the degreasing sterilized is 60-63 ℃, and sterilization time is 10-20 second.
8. the preparation method who replenishes the calcium, locks the calcium health food as claimed in claim 6, when it is characterized in that carrying out ultrafiltration with ultrafiltration membrane, the aperture of milipore filter is 0.001-0.02 μ m, operating pressure is 0.3-1.0 Mpa.
9. the preparation method who replenishes the calcium, locks the calcium health food as claimed in claim 6 is characterized in that described softwood is put into oscillating granulator to be crossed the 16-30 mesh sieve and process wet granular, and 50-60 ℃ dry 2-4 hour down, cross the 16-25 mesh sieve and must do particle.
10. the preparation method who replenishes the calcium, locks the calcium health food as claimed in claim 6 is characterized in that described product is granule, tablet or capsule.
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| CN2011101556923A CN102302116B (en) | 2011-06-10 | 2011-06-10 | Calcium supplementing and calcium locking health care food and preparation method thereof |
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| CN2011101556923A CN102302116B (en) | 2011-06-10 | 2011-06-10 | Calcium supplementing and calcium locking health care food and preparation method thereof |
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