CN102302116B - Calcium supplementing and calcium locking health care food and preparation method thereof - Google Patents
Calcium supplementing and calcium locking health care food and preparation method thereof Download PDFInfo
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Abstract
The invention provides a calcium supplementing and calcium locking health care food. The calcium supplementing and calcium locking health care food comprises calcium, colostrum basic protein, chondroitin sulfate, D-glucosamine, vitamin D, casein phosphopeptides and the like, wherein the colostrum basic protein is obtained by degreasing, separating, concentrating, removing casein, alpha-lactoalbumin, beta-lactoglobulin and substances with molecular weights of more than 30 kDa and less than 1 kDa and drying at a low temperature; the biggest difference between the calcium supplementing and calcium locking health care food prepared by the method provided by the invention and the prior art is that the capacity of the bone tissue in actively absorbing osteoblast nutrition like calcium, ossein and the like can be effectively improved and effects of activating osteoblasts and inhibiting osteoclast activity to enhance bone mineral density and length and promote the health of the bone are obtained. The calcium supplementing and calcium locking health care food provided by the invention, which is a latest generation of domestic osteoarthrosis rehabilitation product, obtains breakthrough in the calcium supplementing effect, can be also used for helping the bone to preferably absorb the calcium in the food, has no toxic and side effect after being taken for a long time.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof.Be particularly related to a kind of just milk basic protein composition that contains, be used to the Ricipe for health care food of lock calcium and preparation method thereof of replenishing the calcium.
Background technology
Due to the impact of dietary structure, calcium deficiency is one of nutrition problem of facing for a long time of Chinese, for calcium supplementing product, has formed huge market thus.But for many years, calcium supplementing product develops around passive supplementary various calcium agent substantially, and the negative effect that effective absorptivity problem of various calcium agent and excessively supplementary calcium agent bring also becomes the focus of paying close attention in recent years, for example: excessive interpolation is calcareous or carelessly add calcareously, and now the calcium content of part calcium supplementing product suggestion dose every day that surpasses U.S. RDI even surpasses the high level of 1000 milligrams on the market; Or coveting to replenish the calcium contains and in the food that oxalic acid is higher, carelessly add calcareously at some, declare high-calcium formula.Long-term edible these high calcium products can the oxalic acid in food be combined oxalic calcium and be deposited in vivo, form the even renal lesions such as chronic renal failure of kidney stone, ephritis.
Outer state-owned many research discoveries, skeleton bulking disease patient surpasses five to ten years if taken anti-bone-loss medicine such as bisphosphonates, just may cause the atypia fracture.The researcher studies this sick pathology, after microscopic examination, finds that the firmness degree of atypia fracture patient sclerotin is as good as with old bone.Originally, anti-bone-loss medicine can reduce bone-loss, only suppress broken bone function, have no idea to manufacture new bone, under long-term the use, in a disguised form by the residual body of old bone, be that old bone need run off through metabolism originally, but medicine retains old bone, even so that in patient body sclerotin density high again, but bone is still unbearably fragile, and risk of bone fracture goes up not down.Make a general survey of global bone health care course, roughly can be divided into three phases: the first stage is to replenish the calcium merely; Second stage is to replenish the calcium to add to supplement the function factor stage that promotes that calcium absorbs, and if added vitamin D, promotes the absorption of calcium, and the phase III is comprehensive reparation and the regeneration stage of bone.Nearly all strong bone product all is in second stage at present.
Calcareous and bone collagen is all the main material of building bone, and calcareous meeting is attached to the firm bone of formation on bone collagen.The gelatine proper energy helps to absorb calcareous, defends simultaneously calcium loss, and human body need to absorb enough calcareous and bone collagens and manufacture bone.In people's children and adolescence, osteoblast is more active than osteoclast, belongs to the growth period of bone, and the people constantly grows tall; After entering and growing up, osteoblast and osteoclast keep balance, and bone stops growing; And after entering old age, osteoclast is more active than osteoblast, causes a large amount of calcium loss, thereby form osteoporosis.Under the microscope, the bone of osteoporosis occurs, the bone of the obvious compared with normal of its bone trabecula is few, and it is large that hole becomes, and the density of bone descends, and it is fragile that sclerotin becomes.
Summary of the invention
The defect of body malabsorption in order to overcome the unilateral accumulation calcium in human body of existing calcium supplementing product, the invention provides a kind of contain first active ingredients such as milk basic protein, calcium, chondroitin sulfate, D-Glucosamine, CPP replenish the calcium, lock calcium health food and preparation method thereof, product of the present invention can effectively improve the ability that bone tissue active absorption calcium and ossein etc. are made osteotrophy, has the health care of replenishing the calcium, consolidate calcium, lock calcium.
It is of the present invention that to replenish the calcium, lock the calcium health food be formulated by weight percentage by following composition:
Calcium 10-35% is milk basic protein 1-5% just
Chondroitin sulfate 2-10% D-Glucosamine 10-40%
Vitamin D 0.05-0.5% CPP 2-8%
The pregelatinized starch surplus.
Of the present inventionly replenish the calcium, lock the calcium health food and fill a prescription preferably and be:
Calcium 25-35% is milk basic protein 2-5% just
Chondroitin sulfate 5-10% D-Glucosamine 20-40%
Vitamin D 0.1-0.3% CPP 5-8%
The pregelatinized starch surplus.
Calcium described in formula can be one or more of the substantial equivalence compositions such as calcium lactate, milk calcium, calcium pantothenate, calcium carbonate, calcium citrate, calcium acetate, amino acid chelated calcium, calcium gluconae, citric acid and calcium malate, wherein, calcium lactate is white particle or powder, free from extraneous odour, micro-have a weathering, be soluble in hot water, have advantages of that solubility is high, dissolution velocity is fast, bioavailability is high, mouthfeel is good; Chondroitin sulfate is the acid mucopolysaccharide from extraction, purifying gained the animal cartilaginous tissue, it is a kind of macromolecular compound, can strengthen cellular immune function, humoral immune function and non-specific immune function, growth that simultaneously can the induced osteogenesis cell, be adjusted to the osteocyte calcification, promote the calcium deposition, have the effect that strengthens immunity and increase bone density; D-Glucosamine of the present invention can be D-Glucosamine Hydrochloride, can be also the D-aminoglucose sulfate, and the D-Glucosamine is the composition of poly glucosamine in cartilage matrix and synovia.Humans and animals is synthesizing amino glucose in vivo, but along with the increase at age, the speed that biosynthetic speed is unable to catch up with degrading, will lack Glucosamine in body, and then affect cartilage cell's metabolism.Glucosamine has obvious humidification to body specific cellular immunity function, humoral immune function and non-specific immune function, Glucosamine can be by promoting osteoblast activity simultaneously, increase the expression of bone morphogenetic protein, promote the absorption of the trace elements such as calcium, phosphorus, increase the fixation of bone tissue to calcium, avoid calcium loss, thereby effectively increase bone density.Exogenous Glucosamine specific effect is in articular cartilage, can stimulate cartilage cell's synthetic proteins polysaccharide, supplement the missing element of cartilage matrix, recover cartilage cell's eubolism function, enable the proteoglycan of synthetic normal configuration, safeguard the morphosis of cartilage matrix, vitamin D mainly contains following physiological function: improve the absorption of human body to calcium, phosphorus, make the level of plasma calcium and the blood plasma phosphorus degree that reaches capacity, promote growth and bone calcification, promote tooth sound, by the intestines wall, increase the absorption of phosphorus, and increase absorbing again of phosphorus by renal tubule, maintain the normal level of citrate in blood, CPP can promote small intestine to the absorption of calcium, promote the utilization to calcium of bone and tooth, first milk basic protein in the present invention is from normal raising, the healthy cow of non-communicable disease and mammitis divides the milk of extruding in 72 hours puerperiums to separate through degreasing, concentrated, remove casein, α-lactoalbumin, β-lactoglobulin matter and molecular wt are at the material more than 30 kDa and below 1 kDa, through frozen drying or spray-dried after, through ultrafiltration, refine the low-molecular-weight activated protein formed, colostrum is applied over 50 years as a kind of nutrition treasure, colostrum not only protein content is high, and contain the rare albumen do not had in many other dairy products, the present invention adds just milk basic protein in Ricipe for health care food, with chondroitin sulfate, CPP, the synergies such as vitamin D, can make it directly act on bone cells, suppress the osteoclast activity, promote osteoblast quantity to increase and liveness, and the activity of coordination Gegenbaur's cell and osteoclast, the dynamic equilibrium that keeps both, promote bone metabolism, and then reach the promotion bone growth, repair the purpose of sclerotin.
Calcium lactate of the present invention is the auspicious general board calcium lactate of Zhengzhou Ruipu Bioengineering Co., Ltd.; Vitamin D is the newly new synthetic board vitamin D of synthetic limited company of Zhejiang; Glucosamine is the fengrun board D-Glucosamine Hydrochloride of Taizhou City Fengrun Biochemical Co., Ltd; Chondroitin sulfate is the extra large treasure board chondroitin sulfate of Shandong Hai Yu Bioisystech Co., Ltd; CPP is the cosen board CPP that Jiangxi can biochemical Co., Ltd; Pregelatinized starch is the merely medicine board pregelatinized starch of Ahua Pharmaceutical Co., Ltd., Liaocheng, Shandong.
The preparation method who replenishes the calcium, locks the calcium health food of the present invention is as follows:
1) liquid bovine colostrum is adopted to cryogenic freezing centrifuge centrifugation 20-40 minute under the condition of 2～5 ℃ of temperature, rotating speed 3000-5000 rev/min, remove the upper strata fat deposit, obtain the degreasing liquid bovine colostrum that fat content is less than 0.08%.Excess Temperature is unfavorable for centrifugal, and easily destroys the just immune globulin activity factor in Ruzhong, thus temperature be controlled at below 5 ℃, centrifugal effective can't exerting an influence to immunoglobulin (Ig).Afterwards, to the colostrum after degreasing, adopt flash pasteurization to carry out sterilizing, sterilising temp 60-63 ℃, sterilization time 10-20 second;
2) with 1 N hydrochloric acid, degreasing, liquid bovine colostrum after sterilizing being adjusted to the pH value is 4.6, make casein from colostrum, solidifying precipitation, the casein that the rotating speed of take in industrial centrifuge will solidify as the condition of 3000-5000 rev/min is through 30-50 minute centrifugal treating, thereby separates with lactalbumin, nonprotein, lactose, mineral matter etc. in solution.It is that product contains the colloid calcium phosphate identical with original level that centrifugal process is separated caseic advantage, can in water, the form with micella disperse, and its character is also similar to original Micellar properties;
3) remove α-lactoalbumin: pH value to the 3.9 trisodium citric acid set-up procedure 2 of using 1.5M), and be heated to 30-35 ℃ and reach 120-150 minute, after α-lactoalbumin precipitation, remove the α-lactoalbumin of precipitation, centrifugal rotational speed 3000-5000 rev/min by centrifugal filtration;
4) remove β-lactoglobulin matter: with the 1N sodium hydroxide solution, the acid-base value of step 3) is adjusted to pH4.5, then with the speed centrifugal filtration of 18000 rev/mins, the beta lactoglobulin polymerization is removed, this step also can be removed remaining casein;
5) ultrafiltration: with the ultrafiltration membrance filter of 100 kDa and 30 kDa, remove successively the material that molecular wt unit is greater than 100 kDa and 30 kDa continuously.With milipore filter, part molecular wt unit is less than again to other scintillas of 1 kDa, as salt and lactose, remove, obtaining molecular wt is the material of 1 kDa～30 kDa, carries out ultrafiltration with ultrafiltration membrane, and the range of structures in the hole of milipore filter is 0.001-0.02 μ m, can be by macromolecular substances such as protein, fat globule, polysaccharide etc. are held back, part lactose and moisture, ash content is as seeing through thing by film, and operating pressure is generally 0.3-1.0 Mpa;
6) adopt existing hypothermic lyophilization technology or spray drying technology to carry out drying to step 5), by stand-by after dried just milk basic protein particle grinding, should concentrate just milk basic protein Stability Analysis of Structures, biologically active does not have to change substantially, and protein content is greater than 80%.
7) calcium, chondroitin sulfate, Glucosamine, vitamin D, CPP, pregelatinized starch are crossed respectively to 80 mesh sieves, standby;
8) chondroitin sulfate of formula rate, CPP are mixed in three-dimensional mixer; The mode of progressively increasing by equivalent again adds calcium, Glucosamine, the pregelatinized starch of formula rate successively, fully mixes, and obtains mixed powder; This mixed powder is put in trough type mixing machine, adding percentage by weight is that 20-40%, concentration are 50% ethanolic solution mixing, make softwood, softwood is put into to oscillating granulator 16-30 mesh sieve granulates, obtain wet granular, and, by wet granular dry 2-4 hour under 50-60 ℃, cross the 16-25 mesh sieve, obtain dry particle;
9) vitamin D of formula rate is mixed in three-dimensional mixer with the colostrum basic protein fraction powder of equivalent, the mode increased progressively by equivalent successively again adds remaining colostrum basic protein fraction powder and dry particle, fully mix, obtain total mixture, for avoiding vitamin D and the just change of milk basic protein character in granulation, dry run, therefore take additional mode in the end always in mixed operation, to add;
10) above-mentioned total mixture is pressed to the agent of prior art granulation, tablet or capsule according to the market demand, pack and obtain product of the present invention.
Of the present inventionly replenish the calcium, lock not being both of calcium health food and current other bone health product maximum and can effectively improve the ability that bone tissue active absorption calcium and ossein etc. are made osteotrophy, but not simple supplementary calcium food, thereby first milk basic protein add can make product of the present invention have the activation osteoblast and suppress the osteoclast activity, make calcareous quilt effectively absorb, reduce to run off enhancing bone density and length, the effect of promotion bone health; Calcium supplement health care food of the present invention has not only been obtained breakthrough on the effect of replenishing the calcium, can also help calcareous, long-term edible without any toxic and side effect in assimilating food better of bone, is the latest domestic generation osteoarthropathy good merchantable brand of recuperating.
The specific embodiment
Embodiment 1:
This enforcement is described to be comprised of following ingredients by weight percentage be used to the health food of replenishing the calcium, consolidate calcium, lock calcium:
Calcium lactate 10 kg calcium citrate 15kg
Milk calcium 10kg is milk basic protein 4 kg just
Chondroitin sulfate 10 kg D-aminoglucose sulfate 20 kg
Vitamin D (0.25%) 0.2 kg CPP 8 kg
Pregelatinized starch 22.8 kg
Preparation process is as follows:
1) liquid bovine colostrum is adopted to the centrifugation 30 minutes under the condition of 2～5 ℃ of temperature, 4000 rev/mins of rotating speeds of cryogenic freezing centrifuge, remove the upper strata fat deposit, obtain the degreasing liquid bovine colostrum that fat content is less than 0.08%.Afterwards, to the colostrum after degreasing, adopt flash pasteurization to carry out sterilizing, sterilising temp 60-63 ℃, sterilization time 15 seconds;
2) with 1 N hydrochloric acid, degreasing, liquid bovine colostrum after sterilizing being adjusted to the pH value is 4.6, makes casein from colostrum, solidifying precipitation, and the casein that the rotating speed of take in industrial centrifuge will solidify as the condition of 4000 rev/mins was through 40 minutes centrifugal treating;
3) remove α-lactoalbumin: pH value to the 3.9 trisodium citric acid set-up procedure 2 of using 1.5M), and be heated to 30-35 ℃ and reach 120 minutes, after α-lactoalbumin precipitation, remove the α-lactoalbumin of precipitation, 4000 rev/mins of centrifugal rotational speeds by centrifugal filtration;
4) remove β-lactoglobulin matter: with the 1N sodium hydroxide solution, the acid-base value of step 3) is adjusted to pH4.5, then with the speed centrifugal filtration of 18000 rev/mins, the beta lactoglobulin polymerization is removed;
5) ultrafiltration: with the ultrafiltration membrance filter of 100 kDa and 30 kDa, remove successively the material that molecular wt unit is greater than 100 kDa and 30 kDa continuously.With milipore filter, part molecular wt unit is less than to other scintillas of 1 kDa again, removes as salt and lactose, obtaining molecular wt is the material of 1 kDa～30 kDa.Operating pressure is controlled at the 0.5-0.8Mpa scope;
6) adopt existing hypothermic lyophilization technology to carry out drying to step 5), and by stand-by after dried just milk basic protein particle grinding;
7) calcium lactate, milk calcium, calcium citrate, chondroitin sulfate, D-Glucosamine Hydrochloride, vitamin D, CPP, pregelatinized starch are crossed respectively to 80 mesh sieves, standby;
8) chondroitin sulfate of formula rate, CPP are mixed in three-dimensional mixer; The mode of progressively increasing by equivalent again adds calcium lactate, milk calcium, calcium citrate, D-Glucosamine Hydrochloride, the pregelatinized starch of formula rate successively, fully mixes, and obtains mixed powder; This mixed powder is put in trough type mixing machine, added that percentage by weight is 30%, concentration is that 50% ethanolic solution mixes, and makes softwood, softwood is put into to oscillating granulator 16 mesh sieves and granulate, obtain wet granular, and by wet granular under 50 ℃ dry 4 hours, cross 16 mesh sieves, obtain dry particle;
9) vitamin D of formula rate is mixed in three-dimensional mixer with the colostrum basic protein fraction powder of equivalent, then the mode increased progressively by equivalent successively adds remaining colostrum basic protein fraction powder and dry particle, fully mix, obtain total mixture;
10) total mixture is put to compressing tablet in rotary tablet machine, selected, reject defective work, pack and obtain tablet product of the present invention.
Embodiment 2:
The described functional food of replenishing the calcium, consolidate calcium, lock calcium of this enforcement consists of by weight percentage following ingredients:
Calcium carbonate 25 kg are milk basic protein 5kg just
Chondroitin sulfate 5 kg D-Glucosamine Hydrochloride 40 kg
Vitamin D (0.25%) 0.3 kg CPP 5 kg
Pregelatinized starch 19.7 kg
Preparation process is as follows:
1) liquid bovine colostrum is adopted to the centrifugation 20 minutes under the condition of 2～5 ℃ of temperature, 5000 rev/mins of rotating speeds of cryogenic freezing centrifuge, remove the upper strata fat deposit, obtain the degreasing liquid bovine colostrum that fat content is less than 0.08%.Afterwards, to the colostrum after degreasing, adopt flash pasteurization to carry out sterilizing, sterilising temp 60-63 ℃, sterilization time 20 seconds;
2) with 1 N hydrochloric acid, degreasing, liquid bovine colostrum after sterilizing being adjusted to the pH value is 4.6, makes casein from colostrum, solidifying precipitation, and the casein that the rotating speed of take in industrial centrifuge will solidify as the condition of 5000 rev/mins was through 30 minutes centrifugal treating;
3) remove α-lactoalbumin: pH value to the 3.9 trisodium citric acid set-up procedure 2 of using 1.5M), and be heated to 30-35 ℃ and reach 150 minutes, after α-lactoalbumin precipitation, remove the α-lactoalbumin of precipitation, 3000 rev/mins of centrifugal rotational speeds by centrifugal filtration;
4) with embodiment 1;
5) with embodiment 1;
6) adopt existing spray drying technology to carry out drying to step 5), and by stand-by after dried just milk basic protein particle grinding;
7) calcium carbonate of formula rate, chondroitin sulfate, D-Glucosamine Hydrochloride, vitamin D, CPP, pregelatinized starch are crossed respectively to 80 mesh sieves, standby;
8) chondroitin sulfate of formula rate, CPP are mixed in three-dimensional mixer; The mode of progressively increasing by equivalent again adds pregelatinized starch, calcium carbonate, the D-Glucosamine Hydrochloride of formula rate successively, fully mixes, and obtains mixed powder; This mixed powder is put in trough type mixing machine, added that percentage by weight is 20%, concentration is that 50% ethanolic solution mixes, and makes softwood, softwood is put into to oscillating granulator 25 mesh sieves and granulate, obtain wet granular, and by wet granular under 60 ℃ dry 2 hours, cross 20 mesh sieves, obtain dry particle;
9) with embodiment 1;
10) by total mixture filled capsules, the polishing of step 9), select the removal defective work, pack and obtain capsule product of the present invention.
Embodiment 3:
The described functional food of replenishing the calcium, consolidate calcium, lock calcium of this enforcement consists of by weight percentage following ingredients:
Amino acid chelated calcium 10 kg calcium gluconae 10kg
Calcium acetate 10 kg are milk basic protein 2 kg just
Chondroitin sulfate 8 kg D-Glucosamine Hydrochloride 30 kg vitamin D (0.25%) 0.1 kg CPP 5 kg pregelatinized starch 24.9 kg
Preparation process is as follows:
1) liquid bovine colostrum is adopted to the centrifugation 40 minutes under the condition of 2～5 ℃ of temperature, 3000 rev/mins of rotating speeds of cryogenic freezing centrifuge, remove the upper strata fat deposit, obtain the degreasing liquid bovine colostrum that fat content is less than 0.08%.Afterwards, to the colostrum after degreasing, adopt flash pasteurization to carry out sterilizing, sterilising temp 60-63 ℃, sterilization time 20 seconds.
2) with 1 N hydrochloric acid, degreasing, liquid bovine colostrum after sterilizing being adjusted to the pH value is 4.6, makes casein from colostrum, solidifying precipitation, and the casein that the rotating speed of take in industrial centrifuge will solidify as the condition of 3000 rev/mins was through 50 minutes centrifugal treating;
3) remove α-lactoalbumin: pH value to the 3.9 trisodium citric acid set-up procedure 2 of using 1.5M), and be heated to 30-35 ℃ and reach 130 minutes, after α-lactoalbumin precipitation, remove the α-lactoalbumin of precipitation, 5000 rev/mins of centrifugal rotational speeds by centrifugal filtration;
4), 5), 6) all with embodiment 1;
7) amino acid chelated calcium of formula rate, calcium gluconae, calcium acetate, chondroitin sulfate, D-Glucosamine Hydrochloride, vitamin D, CPP, pregelatinized starch are crossed respectively to 80 mesh sieves, standby;
8) chondroitin sulfate of formula rate, CPP are mixed in three-dimensional mixer; The mode of progressively increasing by equivalent again adds amino acid chelated calcium, calcium gluconae, calcium acetate, pregelatinized starch, the D-Glucosamine Hydrochloride of formula rate successively, fully mixes, and obtains mixed powder; This mixed powder is put in trough type mixing machine, added that percentage by weight is 40%, concentration is that 50% ethanolic solution mixes, and makes softwood, softwood is put into to oscillating granulator 30 mesh sieves and granulate, obtain wet granular, and by wet granular under 55 ℃ dry 3 hours, cross 25 mesh sieves, obtain dry particle;
9) with embodiment 1;
10) by the total mixture of step 9) packing, obtain granule product of the present invention.
The embodiment of the present invention 1, embodiment 2, embodiment 3 have been carried out respectively to Toxicological evaluation, and result of the test shows: the test of SD rat acute per os shows that the three is nontoxic level formula; Salmonella reversion test, mouse marrow cell micro nuclear test and three genetic toxicity tests of mouse sperm deformity test are negative findings, show that three Example formulations are all without mutagenesis, at maximum dose level, in 30 days feeding trials of the rat of 100 times of human body recommended intakes, do not cause the ANOMALOUS VARIATIONS of rat holistic health, biochemical functions and the various indexs of organ-tissue morphology.
The embodiment of the present invention 1, embodiment 2, embodiment 3 have been carried out respectively to the animal functional trial, and experimental animal adopts the clean level female sd inbred rats of having excised ovary.Three embodiment press respectively the gavage liquid of 10 times of amounts of human body RD by distilled water preparation respective concentration, as experimental group, model control group and sham-operation group (not spay group) give respectively isopyknic distilled water, the positive controls per os gives the Allan sodium phosphate of 1.0mg/kgbw, give respectively the animal subject gavage, every day gavage once, the gavage volume is 1.0ml/100gbw.The Allan sodium phosphate is the bone metabolism regulators, can suppress osteoclast activity, thereby plays the effect that bone absorbs that suppresses.For the treatment of postmenopausal osteoporosis, its main adverse reaction is gastrointestinal reaction such as nausea,vomiting,diarrhea etc. clinically.
From on-test, each treated animal all single cage is raised, and feeds and raises identical feed, and gavage gives the test solution that is subjected to separately, drinks deionized water, records food-intake.3 totally months.During off-test, rat is put to death in the cervical vertebra dislocation, through 105 ℃ of bakings 8 hours, after being cooled to room temperature, with the bone mineral measuring instrument, measures the bone density (BMD) of femur midpoint and distal end.After the gross weight of weighing femur, put into the triangular flask through salt acid treatment dry cleansing, add nitric acid+perchloric acid (4:1) mixed acid 15ml, after digestion, use the deionized water constant volume, measure calcium content of bone with atomic absorption spectrophotometer.
Result of the test shows: each organizes the femur length no significant difference (P > 0.05) of rat; Rats in sham-operated group femur distal end bone density, femur center bone density are significantly higher than oophorectomize+group of solvents (P<0.05) (model control group), show the modeling success; Three embodiment group femur center bone densities, femur distal end bone density, femur dry weight, femur calcium contents all are significantly higher than model control group (P<0.05), the results are shown in Table 1.Result shows that three embodiment groups all have preferably and replenishes the calcium, bone calcium, increases the function of rat bone density.
The impact of table 1 on rat femur length and bone density
Claims (1)
1. replenish the calcium, lock the calcium health food for one kind, formulated by weight percentage by following composition:
Calcium 25-35% is milk basic protein 2-5% just
Chondroitin sulfate 5-10% D-Glucosamine 20-40%
Vitamin D 0.1-0.3% CPP 5-8%
The pregelatinized starch surplus
Wherein: described calcium is selected from one or more of calcium lactate, milk calcium, calcium pantothenate, calcium carbonate, calcium citrate, calcium acetate, amino acid chelated calcium, calcium gluconae, citric acid and calcium malate, described D-Glucosamine is D-Glucosamine Hydrochloride or D-aminoglucose sulfate, described just milk basic protein is the 1-30kDa low-molecular-weight activated protein from refining the colostrum whey.
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