CN102173907A - Preparation method of algae amino acid microbial fertilizer - Google Patents
Preparation method of algae amino acid microbial fertilizer Download PDFInfo
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Abstract
The invention discloses a preparation method of an algae amino acid microbial fertilizer, which comprises the following steps: (1) crushing algae, adding water and a fermentation promoter, sterilizing for 20-40 minutes at the temperature of 120-125 DEG C, reducing the temperature to room temperature, adding a composite strain to ferment the mixed solution for 3-5 days at 25-35 DEG C, and adjusting the pH value to 7.2-8.0; and (2) sequentially adding urea and/or monopotassium phosphate and chelating-state trace elements into the solution obtained in the step (1) in a stirring state, and stirring for 20-50 minutes to obtain the liquid algae amino acid microbial fertilizer. According to the invention, algae is subjected to deep fermentation by use of a high-activity microbial agent, the natural active ingredients in algae are perfectly maintained; and due to compatibility with the chelating-state trace elements, the nutrient elements and utilization rate of the fertilizer are improved, and the algae amino acid microbial fertilizer is a novel algae microbial fertilizer for bioremediation and soil adjustment and conforming to ecological balance of animals, plants and microorganisms.
Description
Technical field
The present invention relates to the bio-fertilizer technical field, relate in particular to a kind of preparation method of seaweed amino acid microorganism fertilizer.
Background technology
In recent years, along with expanding economy, people are more and more higher to the attention degree of living environment and quality of life, and food-safety problem also more and more is subjected to social each side extensive concern.These variations have proposed challenge to original agriculture production pattern, seek the focus that agricultural measures new, that meet the agricultural sustainable development needs becomes current agriculture production research.
All the time, fertilizer practice is to ensure one of important means that agriculture production is carried out smoothly.Yet the life-time service of a large amount of mineral manure and unreasonable measures of fertilizer can cause the fertilizer pollution in the agricultural-food, make the farm crop growing way not good enough, the ability drop of opposing harmful organism, the usage quantity and the number of times of agricultural chemicals in the agricultural-food production process have been strengthened, further reduced the quality of agricultural-food, made that pesticide residue exceed standard in the farm crop, the accumulation of soil toxic substance, agricultural environment pollution aggravates, and human and livestock health safety is constituted a threat to etc.Studies on Sustainable Crop Protection (Sustainable Crop Protection in the world wide under this background; SCP) cry grows to even greater heights; organic, pollution-free food production becomes the direction of the future of agriculture development, correspondingly the exploitation of the new source of manure is had higher requirement.
Recovery technique for contaminate environment comprises three major types such as physical method, chemical process and biological method.Wherein biological restoration has become the important component part of environmental protection technology.Biological restoration (Bioremediation) is to utilize biological life Metabolic activity to reduce to be stored in the concentration of hazardous and noxious substances in the environment or to make it innoxious fully, makes polluted environment can partially or completely return to the process of virgin state.Therefore bioremediation agents that research and development is good or soil redeposition become the focus that people pay close attention to.
Marine alga is the low plant such as phototrophy such as grade that is grown in the ocean, is the organic original producer in ocean.In this special environment of ocean, it also is rich in trace elements such as potassium, iodine, manganese except the nutrition that contains land plant and have, and miscellaneous VITAMIN.The objectionable impuritiess such as heavy metal ion of special composition Sargassum polysaccharides in the marine alga in can not only chelating soil, and can also increase the venting capability of soil, soil is difficult for by erodsion losses such as wind, current.Seaweed fertilizer as fertilizer sources is the earliest used in kingdom of ancient Roman, apart from the modern history that nearly one thousand years are arranged, but just really obtains paying attention to and promoting up to the modern times.With marine alga or its extract is that the fertilizer of raw material is called as seaweed fertilizer, and market is called as trehalose, seaweed powder, varec abroad, has been widely used in aspects such as agricultural and gardening abroad, and domestic and abroad market prospect is very good.
People have carried out a large amount of research to marine alga, have developed a large amount of marine alga series fertilizer.The method of existing production marine alga generally is a chemical process, utilizes strong acid and strong base under the violent condition of High Temperature High Pressure marine alga to be handled exactly, and this method technology is comparatively complicated, the condition harshness, and destroyed a large amount of effective constituent in the marine alga.
Summary of the invention
Technical problem to be solved by this invention is: at the deficiency that prior art exists, provide a kind of production technique can not destroy simply, that nutrition in effective constituent in the marine alga, the fertilizer is complete, the preparation method of the seaweed amino acid microorganism fertilizer of environmental protection.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of preparation method of seaweed amino acid microorganism fertilizer may further comprise the steps:
(1) marine alga is pulverized, the water that adds 1～5 times of volume of described marine alga, reach and the fermentation accelerant of marine alga with volume, in temperature is 120～125 ℃ of sterilization 20～40min, drop to room temperature, add composite bacteria, fermentation accelerant and the composite bacteria content in mixing solutions is 0.5～5wt%, 25～35 ℃ of fermentations 3～5 days, regulating the pH value was 7.2～8.0 with mixing solutions.
(2) add urea, potassium primary phosphate and chelated microelements successively under the whipped state in the solution that step (1) obtains, the content that adds the back three is followed successively by 5～20wt%, 5～30wt% and 0.1～5wt%; Or adding urea or potassium primary phosphate and chelated microelements successively, the content that adds urea or potassium primary phosphate and chelated microelements is followed successively by 5～20wt% or 5～30wt% and 0.1～5wt%; Stir 20～50min then, make liquid seaweed amino acid microorganism fertilizer.
Perhaps, after the solution that step (1) is obtained filters, add urea, potassium primary phosphate and chelated microelements successively in the solution that step (1) obtains again under whipped state, the content that adds the back three is followed successively by 5～20wt%, 5～30wt% and 0.1～5wt%; Or adding urea or potassium primary phosphate and chelated microelements successively, the content that adds urea or potassium primary phosphate and chelated microelements is followed successively by 5～20wt% or 5～30wt% and 0.1～5wt%; Stir 20～50min then, with plant ash, the peat composed of rotten mosses or peat soil absorption, make solid seaweed amino acid microorganism fertilizer again.
Be ground into 100 orders when wherein, described marine alga is pulverized.
Wherein, described fermentation accelerant is at least a in extractum carnis, peptone, Semen Maydis powder, soybean cake powder, purple perilla juice and the bamboo leachate.
Preferably, described fermentation accelerant is extractum carnis, peptone, Semen Maydis powder, soybean cake powder, purple perilla juice and bamboo leachate, wherein extractum carnis: peptone: Semen Maydis powder: soybean cake powder: purple perilla juice: the weight ratio of bamboo leachate is 1～2: 0.2～0.5: 5～8: 1～3: 0.1～0.2: 0.5～1.When fermentation accelerant is wherein two kinds or when two or more, the adding weight percent of each fermentation accelerant component is suitable for it and is all adding fashionable corresponding adding ratio.
Described composite bacteria comprises volume ratio 1: 1: 1～2: 1～2 actinomycetes, photosynthetic bacteria, enzymatic microorganism and genus bacillus.
Described chelated microelements comprises at least a in ferric sulfate, manganous sulfate, copper sulfate, zinc sulfate, boric acid and the ammonium molybdate.
Preferably, described chelated microelements comprises ferric sulfate, manganous sulfate, copper sulfate, zinc sulfate, boric acid and ammonium molybdate, wherein ferric sulfate: manganous sulfate: copper sulfate: zinc sulfate: boric acid: the weight ratio of ammonium molybdate is 2～3: 0.5～1: 1～2: 2～3: 1～2: 0.5～1.When chelated microelements is wherein two kinds or when two or more, the adding weight percent of each chelated microelements is suitable for it and is all adding fashionable corresponding adding ratio.
Wherein, described marine alga is at least a in sea-tangle, sargassun, black wrack, the Enteromorpha.
Owing to adopted technique scheme, the invention has the beneficial effects as follows:
The present invention is raw material with the marine alga, utilize the high-activity microorganism microbial inoculum that marine alga is carried out submerged fermentation, effective constituent in the marine alga is dissolved out fully, do not add any strong acid and strong base, kept the natural radioactivity effective constituent in the marine alga greatly, as amino acid, Sargassum polysaccharides, N.F,USP MANNITOL and iodine, potassium, magnesium, manganese, trace elements such as titanium, the genus bacillus that the present invention adds, thalline such as enzymatic microorganism self synthesize α-Dian Fenmei, proteolytic enzyme, lipase, enzymes such as cellulase, the alginic cell wall is broken, dissolving, effective constituent in the marine alga is discharged fully, subtilis can also secrete antimicrobial substance, suppress the growth of harmful bacterium, and then the promotion plant-growth, photosynthetic bacterium can increase photosynthesis of plants, improves the cold-resistant of crop, resistance such as drought resisting; The present invention and chelated microelements compatibility, the nutritive element and the utilization ratio of fertilizer have been significantly improved, nutrition is complete in the seaweed amino acid microorganism fertilizer of making thus, the material that contains multiple promotion plant-growth, not only can strengthen the photosynthesis of crop, it is cold-resistant to strengthen crop, resistance such as drought resisting, promote root system development, make soil ventilative, soft, improve crop yield and quality greatly, the more important thing is farm crop, environment and human no any detrimentally affect and pollution are the new green environment protection fertilizer that adapts to modern agriculture, are to meet animal, plant, the marine alga microbial fertilizer that microbial ecological equilibrated new bio is repaired and soil is regulated.
Production extraction process of the present invention refining, and cost is low can be handled marine alga in the short period of time on a large scale, as ocean and environment are all had very big pollution, people are called the Enteromorpha of " green evil spirit ".And utilize the microbiological deterioration technology to make seaweed fertilizer and also have advantages such as mild condition, facility investment be little.
Embodiment
Further set forth the present invention below in conjunction with specific embodiment.
Embodiment 1
Seaweed powder is broken into 100 orders, the water that adds 2 times of volumes of described marine alga, reach and the fermentation accelerant of marine alga with volume, described fermentation accelerant is that weight ratio is 1: 0.3: 6: 1: 0.1: 0.5 extractum carnis, peptone, Semen Maydis powder, soybean cake powder, purple perilla juice and bamboo leachate, in temperature is 120 ℃ of sterilization 40min, drop to room temperature, add composite bacteria, described composite bacteria is that volume ratio is 1: 1: 1: 1 actinomycetes, photosynthetic bacteria, enzymatic microorganism and genus bacillus, add secondary fermentation promotor and the content of composite bacteria in mixing solutions and be respectively 1.5wt% and 5wt%, 28 ℃ of fermentations 5 days, regulating the pH value was 7.2 with mixing solutions.
In above-mentioned solution, add urea, potassium primary phosphate and chelated microelements under the whipped state successively, described chelated microelements is that weight ratio is 3: 1: 2: 3: 2: 1 ferric sulfate, manganous sulfate, copper sulfate, zinc sulfate, boric acid and ammonium molybdate, the content that adds the back three is followed successively by 15wt%, 8wt% and 0.5wt%, stir 30min then, make liquid seaweed amino acid microorganism fertilizer.
Embodiment 2
Seaweed powder is broken into 100 orders, the water that adds 4 times of volumes of described marine alga, reach and the fermentation accelerant of marine alga with volume, described fermentation accelerant is that weight ratio is 2: 0.5: 5: 3 extractum carnis, peptone, Semen Maydis powder and soybean cake powder, in temperature is 125 ℃ of sterilization 20min, drop to room temperature, add composite bacteria, described composite bacteria is that volume ratio is 1: 1: 2: 2 actinomycetes, photosynthetic bacteria, enzymatic microorganism and genus bacillus, add secondary fermentation promotor and the content of composite bacteria in mixing solutions and be respectively 4.5wt% and 1wt%, 35 ℃ of fermentations 3 days, regulating the pH value was 7.5 with mixing solutions.
In above-mentioned solution, add urea and chelated microelements under the whipped state successively, described chelated microelements is that weight ratio is 2: 0.5: 1: 2: 1: 0.5 ferric sulfate, manganous sulfate, copper sulfate, zinc sulfate, boric acid and ammonium molybdate, add both content of back and be respectively 10wt%, 4.5wt%, stir 40min then, make liquid seaweed amino acid microorganism fertilizer.
Embodiment 3
Seaweed powder is broken into 100 orders, the water that adds 3 times of volumes of described marine alga, reach and the fermentation accelerant of marine alga with volume, described fermentation accelerant is weight ratio 1: 0.3: 7: 2: 0.15: 0.8 extractum carnis, peptone, Semen Maydis powder, soybean cake powder, purple perilla juice and bamboo leachate, in temperature is 122 ℃ of sterilization 30min, drop to room temperature, add composite bacteria, described composite bacteria is that volume ratio is 1: 1: 1.5: 1 actinomycetes, photosynthetic bacteria, enzymatic microorganism and genus bacillus, add secondary fermentation promotor and the content of composite bacteria in mixing solutions and be respectively 2.5wt% and 3wt%, 30 ℃ of fermentations 4 days, regulating the pH value was 8.0 with mixing solutions.
To in the solution that step (1) obtains, add urea and potassium primary phosphate and chelated microelements under the whipped state successively again after the above-mentioned solution filtration, described chelated microelements is that weight ratio is 2: 0.5: 2: 3: 1 ferric sulfate, manganous sulfate, copper sulfate, zinc sulfate, the content that adds the back three is followed successively by 15wt%, 20wt% and 0.1wt%, stir 20min then, with plant ash absorption, make solid seaweed amino acid microorganism fertilizer again.
Embodiment 4
Seaweed powder is broken into 100 orders, the water that adds 5 times of volumes of described marine alga, reach and the fermentation accelerant of marine alga with volume, described fermentation accelerant is that weight ratio is 1: 0.5 extractum carnis and a peptone, in temperature is 124 ℃ of sterilization 30min, drop to room temperature, add composite bacteria, described composite bacteria is that volume ratio is 1: 1: 1: 1 actinomycetes, photosynthetic bacteria, enzymatic microorganism and genus bacillus, add secondary fermentation promotor and the content of composite bacteria in mixing solutions and be respectively 5wt% and 2wt%, 32 ℃ of fermentations 4 days, regulating the pH value was 7.6 with mixing solutions.
To in the solution that step (1) obtains, add potassium primary phosphate and chelated microelements successively under the whipped state again after the above-mentioned solution filtration, described chelated microelements is that weight ratio is 2: 0.5: 2 ferric sulfate, manganous sulfate and a copper sulfate, add both content of back and be followed successively by 25wt% and 2wt%, stir 40min then, with peat composed of rotten mosses absorption, make solid seaweed amino acid microorganism fertilizer again.
Claims (9)
1. the preparation method of a seaweed amino acid microorganism fertilizer is characterized in that may further comprise the steps:
(1) marine alga is pulverized, the water that adds 1～5 times of volume of described marine alga, reach and the fermentation accelerant of marine alga with volume, in temperature is 120～125 ℃ of sterilization 20～40min, drop to room temperature, add composite bacteria, fermentation accelerant and the composite bacteria content in mixing solutions is 0.5～5wt%, 25～35 ℃ of fermentations 3～5 days, regulating the pH value was 7.2～8.0 with mixing solutions;
(2) add urea, potassium primary phosphate and chelated microelements successively under the whipped state in the solution that step (1) obtains, the content that adds the back three is followed successively by 5～20wt%, 5～30wt% and 0.1～5wt%; Or adding urea or potassium primary phosphate and chelated microelements successively, the content that adds back urea or potassium primary phosphate and chelated microelements is followed successively by 5～20wt% or 5～30wt% and 0.1～5wt%; Stir 20～50min then, make liquid seaweed amino acid microorganism fertilizer.
2. the preparation method of seaweed amino acid microorganism fertilizer as claimed in claim 1, it is characterized in that: after the solution that step (1) is obtained filters, add urea, potassium primary phosphate and chelated microelements successively in the solution that step (1) obtains again under whipped state, the content that adds the back three is followed successively by 5～20wt%, 5～30wt% and 0.1～5wt%; Or adding urea or potassium primary phosphate and chelated microelements successively, the content that adds back urea or potassium primary phosphate and chelated microelements is followed successively by 5～20wt% or 5～30wt% and 0.1～5wt%; Stir 20～50min then, with plant ash, the peat composed of rotten mosses or peat soil absorption, make solid seaweed amino acid microorganism fertilizer again.
3. the preparation method of seaweed amino acid microorganism fertilizer as claimed in claim 1 is characterized in that: described marine alga is ground into 100 orders when pulverizing.
4. the preparation method of seaweed amino acid microorganism fertilizer as claimed in claim 1 is characterized in that: described fermentation accelerant is at least a in extractum carnis, peptone, Semen Maydis powder, soybean cake powder, purple perilla juice and the bamboo leachate.
5. the preparation method of seaweed amino acid microorganism fertilizer as claimed in claim 4, it is characterized in that: described fermentation accelerant is extractum carnis, peptone, Semen Maydis powder, soybean cake powder, purple perilla juice and bamboo leachate, wherein extractum carnis: peptone: Semen Maydis powder: soybean cake powder: purple perilla juice: the weight ratio of bamboo leachate is for being 1～2: 0.2～0.5: 5～8: 1～3: 0.1～0.2: 0.5～1.
6. the preparation method of seaweed amino acid microorganism fertilizer as claimed in claim 1 is characterized in that: described composite bacteria comprises that volume ratio is 1: 1: 1～2: 1～2 actinomycetes, photosynthetic bacteria, enzymatic microorganism and genus bacillus.
7. the preparation method of seaweed amino acid microorganism fertilizer as claimed in claim 1 is characterized in that: described chelated microelements comprises at least a in ferric sulfate, manganous sulfate, copper sulfate, zinc sulfate, boric acid and the ammonium molybdate.
8. the preparation method of seaweed amino acid microorganism fertilizer as claimed in claim 7, it is characterized in that: described chelated microelements comprises ferric sulfate, manganous sulfate, copper sulfate, zinc sulfate, boric acid and ammonium molybdate, wherein ferric sulfate: manganous sulfate: copper sulfate: zinc sulfate: boric acid: the weight ratio of ammonium molybdate is 2～3: 0.5～1: 1～2: 2～3: 1～2: 0.5～1.
9. as the preparation method of described any seaweed amino acid microorganism fertilizer of claim 1 to 8, it is characterized in that: described marine alga is at least a in sea-tangle, sargassun, black wrack, the Enteromorpha.
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Application publication date: 20110907 |