CN102171364A - 流体检测试片及其测试方法 - Google Patents
流体检测试片及其测试方法 Download PDFInfo
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- CN102171364A CN102171364A CN2008801313576A CN200880131357A CN102171364A CN 102171364 A CN102171364 A CN 102171364A CN 2008801313576 A CN2008801313576 A CN 2008801313576A CN 200880131357 A CN200880131357 A CN 200880131357A CN 102171364 A CN102171364 A CN 102171364A
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- Prior art keywords
- fluid
- antibody
- nitrocellulose
- area
- test strip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
- G01N33/541—Double or second antibody, i.e. precipitating antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/817—Enzyme or microbe electrode
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/97—Test strip or test slide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/823—Immunogenic carrier or carrier per se
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
本发明提供一种流体检测试片及其测试方法。流体检测试片包含有具有流道的基板,流道上设置有依序连接的第一流体区、第二流体区与第三流体区。此外,流道具有位于第一流体区的第一抗体、位于第一流体区或第二流体区的醣类材料及过氧化物酶、固定于第二流体区的第二抗体,并与第一抗体辨识同一待测物上相异的抗原决定位置、位于第三流体区中包含有醣氧化酶的受质反应试剂。当流体注入流道后,利用醣氧化酶催化流至第三流体区的醣类材料,并产生出过氧化氢,过氧化氢会受到流至第三流体区的过氧化物酶催化进行反应,产生电学信号。
Description
PCT国内申请,说明书已公开。
Claims (1)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2008/001747 WO2010043074A1 (zh) | 2008-10-17 | 2008-10-17 | 流体检测试片及其测试方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN102171364A true CN102171364A (zh) | 2011-08-31 |
Family
ID=42106191
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2008801313576A Pending CN102171364A (zh) | 2008-10-17 | 2008-10-17 | 流体检测试片及其测试方法 |
Country Status (11)
Country | Link |
---|---|
US (1) | US8133718B2 (zh) |
EP (1) | EP2339019B1 (zh) |
JP (1) | JP2012505417A (zh) |
KR (1) | KR101252953B1 (zh) |
CN (1) | CN102171364A (zh) |
AU (1) | AU2008362975B2 (zh) |
BR (1) | BRPI0823169A2 (zh) |
CA (1) | CA2740514A1 (zh) |
ES (1) | ES2429113T3 (zh) |
RU (1) | RU2477754C2 (zh) |
WO (1) | WO2010043074A1 (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102187218B (zh) | 2008-10-09 | 2014-02-12 | 红电医学科技股份有限公司 | 流体检测方法 |
KR101178009B1 (ko) | 2008-10-17 | 2012-08-28 | 액텀 아이엔씨. | 액체 시험 스트립 및 방법 |
US8301684B2 (en) * | 2009-02-26 | 2012-10-30 | Google Inc. | User challenge using information based on geography or user identity |
US10001449B2 (en) * | 2014-12-15 | 2018-06-19 | Church & Dwight Co., Inc. | Systems, devices and methods for a hydroscopic based lateral flow assay |
Citations (4)
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CN101303358A (zh) * | 2007-05-10 | 2008-11-12 | 胡军 | 虹吸式血糖测试试纸 |
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TWM350706U (en) | 2008-09-23 | 2009-02-11 | Actherm Inc | Testing strip |
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TWM359693U (en) | 2008-10-09 | 2009-06-21 | Actherm Inc | Combinatory testing strip |
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-
2008
- 2008-10-17 EP EP08877351.0A patent/EP2339019B1/en not_active Not-in-force
- 2008-10-17 WO PCT/CN2008/001747 patent/WO2010043074A1/zh active Application Filing
- 2008-10-17 KR KR1020117008111A patent/KR101252953B1/ko not_active IP Right Cessation
- 2008-10-17 JP JP2011531320A patent/JP2012505417A/ja not_active Ceased
- 2008-10-17 ES ES08877351T patent/ES2429113T3/es active Active
- 2008-10-17 BR BRPI0823169-9A patent/BRPI0823169A2/pt not_active IP Right Cessation
- 2008-10-17 RU RU2011118609/10A patent/RU2477754C2/ru not_active IP Right Cessation
- 2008-10-17 CA CA2740514A patent/CA2740514A1/en not_active Abandoned
- 2008-10-17 AU AU2008362975A patent/AU2008362975B2/en not_active Ceased
- 2008-10-17 CN CN2008801313576A patent/CN102171364A/zh active Pending
-
2009
- 2009-04-29 US US12/431,915 patent/US8133718B2/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1334924A (zh) * | 1998-11-17 | 2002-02-06 | 恩特里克斯公司 | 一种检测生物样品中分析物存在的方法 |
CN1309294A (zh) * | 2000-01-21 | 2001-08-22 | 松下电器产业株式会社 | 生物传感器 |
WO2007128286A1 (de) * | 2006-05-08 | 2007-11-15 | 8Sens.Biognostic Gmbh | Verfahren zum gekoppelten enzym-immunchemischen nachweis von analyten mittels endogener kalibratoren |
CN101303358A (zh) * | 2007-05-10 | 2008-11-12 | 胡军 | 虹吸式血糖测试试纸 |
Also Published As
Publication number | Publication date |
---|---|
EP2339019A1 (en) | 2011-06-29 |
WO2010043074A1 (zh) | 2010-04-22 |
BRPI0823169A2 (pt) | 2015-06-23 |
RU2477754C2 (ru) | 2013-03-20 |
US8133718B2 (en) | 2012-03-13 |
ES2429113T3 (es) | 2013-11-13 |
KR20110057217A (ko) | 2011-05-31 |
KR101252953B1 (ko) | 2013-04-15 |
US20100099114A1 (en) | 2010-04-22 |
RU2011118609A (ru) | 2012-11-27 |
EP2339019B1 (en) | 2013-08-14 |
EP2339019A4 (en) | 2012-03-28 |
AU2008362975B2 (en) | 2013-03-28 |
AU2008362975A1 (en) | 2010-04-22 |
JP2012505417A (ja) | 2012-03-01 |
CA2740514A1 (en) | 2010-04-22 |
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