201013183 九、發明說明： 【發明所屬之技術領域】 本發明侧於-種檢職片，_是—種有關於翻於流體檢測的試 片。 【先前技術】 以流體檢測試片進行生化檢測與免疫檢測的習知技術中，流體檢測試片 在其基板絲材上設計核道或織構及表硫疏水性處理，而因流道 〇 腳並麵水㈣，且制流體彡騎杨蛋自質或是_等輯度高之組 成物’所以當待測流體流過後’會在流道上殘留，使得待測流體無法完全反 應，如此-來，不僅造成制流體的浪費，更可能造成最制試結果的誤差。 此外’習知技術的流體檢測試片在流體傳送方面，可設計有微流道結 構，並係利用微流道結構產生的毛細現象，將流體經過流道被動傳送至反應 偵測區域；另一種方式則是在注入待測流體時即利用加壓或真空負壓等方 式，給予流體一驅動力，又或於流道中設置一個或以上之微閥門 (micro-actuator or valve)等設計，使得流體可主動並依序通過流道，到達反應 偵測區域。但是無論是上述任一種方式，待測流體注入流道後常常產生或捲 ® 入大小不一的氣泡使得流道阻塞，造成實際測量上之誤差，甚至致使測試失 敗，而微閥門(micro-actuator or valve)增設又增加整體設計困難度與試片成 本。 又，習知技術的檢測試片在製作上多使用模鑄、射出成型或壓印(imprint) 的方式在基板上做出流道或微流道結構，所以必須使用聚乙稀(PE)、聚氣乙 烯(PVC)或聚丙烯(PP)等價格較高之塑膠聚合物作為材質且模具耗損較快， 進而造成試片之總體成本的提高。 此外，由於進行的反應不同，亦須有不同的反應材料或試劑。習知技術 的檢測試片往往設計成只具有單一試劑或反應材料，故只適用於單一反應及 201013183 樣本同時進行多種測試。若是要進行不同的 ，如此一來，將造成使用上的不便及測試時 單一種類的測試，無法針對單— 測試，則需使用不同的檢測試片 間的耗費。 【發明内容】 域服上賴點’本_提供—種二合—碰蝴制可同時用於生 化檢測與免疫_。主要包含—基板，基板自其上表面向下凹設供生化檢測 的第-流道與供免疫檢測的第二流道，且第一流道與第二流道各自包含依序 連接之第一流體區、第二流體區與第三流體區，第-流體區係供流體之注 Ό 入。^合一流體檢測試片的特徵在於，在第-流道與第二流道的第二流體區 與第二流體區之底部各自戦有槪纖維層，概_層包含有巾空網狀構 型’其中第二流體區係供流體之傳送，第三流體區係供流體之反應。又，第 -流體區的硝化纖維層平均厚度不大鱗三紐區雜_層厚度。在確化 纖維層之中空網狀構型中形成有反應材料。此外，基板具有縱向抽線，使第 一流道與第二流道的第三流體區同時位於縱向轴線上。 因此本發明之主要目的，係提供一種二合一流體檢測試片，可同時對 單一樣本進行生化及免疫檢測。 ❹本發明之另一目的，係提供一種二合一流體檢測試片，因具有可吸收液 體的硝化纖維層，故可避免流道之液體殘留。 本發明之另一目的，係提供一種二合一流體檢測試片，其具有可吸收液 體的确化纖維層；由於單位艘積的頌化纖維吸收液體量係為定值，故可經由 設定基板上硝化纖維層的體積，而提供待測流體的定量檢測。 本發明之又一目的，係提供一種二合一流體檢測試片，具有中空網狀構 型的硝化纖維層，由於流體流經中空網狀構型時，流體中的氣泡會被破壞， 故可消彌較大的氣泡’避免微流道技術中氣泡阻塞流道的狀況發生，進而影 響定量分析結果。 201013183 【實施方式】 由於本發明係揭露一種二合一流體檢測試片，其中所利用物理化學原 理及/谷液塗佈技術，已為相關技術領域具有通常知識者所能明瞭，故以下文 巾之朗再作完整描述。同時，以下文巾所賴之圖式，絲達與本發 明特徵有關之示意，並未亦不需要依據實際情形完整繪製，合先敛明。 請參考第1圖，為本發明所提出之較佳實施例，為一種二合一流體檢測 試片，可同時用於生化檢測與免疫檢測。二合一流體檢測試片丨主要包含有 基板10及支揮件19。基板川自其上表面100向下凹設供生化檢測的第一 流道11與供免疫檢測的第二流道12。第一流道u包含依序連接之第一流體 區m、第二流體區112與第三流體區113。第二流道12則包含有依序連接 之第一流體區121、第二流體區122及第三流體區123。第一流道u的第一 流體區111與第二流道12的第一流體區121互相連通，供流體之注入。當 流體在注入互相通連的第一流體區11丨與12ι後，分別經由第一流道u與 第一流道12的第二流體區112與122的傳送，各自到達第一流道u與第二 流道12的第三流體區113與123 ;而流往第一流道η的流體，流至其第= 流體區113時’流體中的待測成份會在此處進行生化反應，產生訊號以供债 〇 測。同樣地，流向第二流道12的流體，在流至其第三流體區123時，流體 中的待測成份則會在此處進行反應，產生訊號以供偵測。在較佳的實施狀雜 中’基板10為生物相容(biocompatible)材料。 凊繼續參考第2圖’為本發明的二合一流體檢測試片俯視圖。為了便於 偵測，在二合一流體檢測試片1上的第一流道u的第三流體區113與第二 流道12的第三流體區123在設置上是位於基板1〇的同一縱向轴線14上。 如此一來，配合偵測的偵測器只要在同一縱向軸線14上移動即可偵測到第 一流道11的第三流體區113與第二流道12的第三流體區123所發出之反應 訊號。 ’ 請繼續參考第3圖，為第1圖中第一流道U沿八八連線的剖面圖。在 7 201013183 第一流道11的第二流體區112與第三流體區ii3之底部，分別形成有中空 網狀構型的硝化纖維層1121與1131。其中，第一流道^的第二流體區112 的硝化纖維層1121平均厚度Da小於第一流道u的第三流體區113硝化纖 維層1131厚度Db。又在硝化纖維層1121與1131的中空網狀構型中，包含 有反應材料’反應材料的組成係與流體中所含有的待測成份的種類有關。此 外’由於硝化纖維層1121與1131具有多孔性的中空網狀結構，所以可以吸 收由第一流體區111流入的流艎，且流體中的待測成份與存在於硝化纖維層 1131中的反應材料進行反應。 ❹ 請繼續參考第4圖，為第1圖中第二流道12沿BB連線的剖面圖。第 二流道12亦與第一流道11相同，在其第二流體區122、及第三流體區123 的底部分別形成有中空網狀構型的硝化纖維層1221及1231。又在硝化纖維 層1221與1231的中空網狀構型中’與上述第一流道中的硝化纖維層1121 及1131相同，均包含有反應材料，且亦因具有多孔性的中空網狀結構，所 以可以吸收由第一流體區121流入的流體’且流體中的待測成份與存在於硝 化纖維層1231中的反應材料進行反應。 由於第一流道11與第二流道12因具有可吸收液體的硝化纖維層ι121、 113卜1221及1231，故可避免流體殘留於第一流道η與第二流道12之中。 ® 此外，當流體流經具有中空網狀構型的硝化纖維層1121、1131、1221及1231 時，流體中的氣泡會被破壞，故可避免氣泡阻塞第一流道U與第二流道12。 此外，為了降低流道與流體之間的毛細作用所造成的影響，本發明所提 出之第一流道與第二流道並非習知技術所謂的微流道，在設計上，第1圖所 示，第一流道11的第二流體區112寬度Wa、第一流道11的第三流體區113 寬度Wb '第二流道12的第二流體區122寬度Wc，以及第二流道12的第 二流體區123寬度Wd，則是均以至少〇.3mm為佳。 在製作上，硝化纖維層1121、1131、1221及1231的形成方式如下所述。 先將破化纖維粉末(nitrocellulose powder)與含有酯類(ester)和酮類(ketone)的 8 201013183 有機溶劑混合後形成硝化纖維溶液；再將硝化纖維溶液澆注(casting)於第一 流道11的第二流體區112與第三流體區113的底部以及第二流道12的第二 流體區122及第三流體區丨23的底部。經乾燥後，於第一流道n的第二流 體區112底部則會形成頌化纖維層112卜第一流道11的第三流體區113的 底部則形成硝化纖維層1131，第二流道12的第二流體區122的底部則形成 硝化纖維層1221，而於第二流道12的第三流體區123的底部則形成硝化纖 維層123h為達較佳的澆注效果，第一流道u及第二流道12之表面粗糙 度(Ra值)以介於3微米至50微米之間為佳。 瑞化纖維粉末與含有酯類和酮類的有機溶劑混合的較佳體積比例為1 : 9。由於單位體積的硝化纖維吸收液體量係為定值’故可由欲吸收之待測流 體的體積推算出對應的硝化纖維溶液的體積，之後再行澆注，藉此可固定檢 測所需液體之體積量，並適用於微量檢測。 反應材料形成於硝化纖維層1121、1131、1221及1231中的方式則如下 所述。待硝化纖維層1121、1131、1221及1231分別乾燥成形後，將含有反 應材料的反應溶液注入’經過風乾或是冷柬乾燥(ly〇philizati〇n)後，反應材 料則會以粉末狀的形式留存在硝化纖維層1121、1131、1221及1231之中。 反應材料形成於其中的方式除可以先形成硝化纖維層之後再注入反應材料 φ 後的順序形成方式外’亦可將含有反應材料的反應溶液，加入由硝化纖維粉 末(nitrocellulose powder)與含有酯類(ester)和酮類(ketone)的有機溶劑組成的 石肖化纖維溶液中，混合完畢之後’再將混合好的溶液洗注(casting)於第一流 道11的第二流體區112與第三流體區113的底部以及第二流道12的第二流 體區122、第三流體區123的底部，經過風乾或冷凍乾燥程序，同時將硝化 纖維溶液形成硝化纖維層1121、1131、1221及1231，以及將反應材料形成 粉末狀留存在硝化纖維層1121、1131、1221及1231之中。 如上所述’第一流道11係供生化檢測，而第二流道12係供免疫檢測。 由於生化與免疫檢測所需進行之反應有所差異，故形成於第一流道U的硝 9 201013183 化纖維層1121與1131中的反應材料之組成，與形成於第二流道12的硝化 纖維層1221與1231中的反應材料組成亦有所不同。例如，進行生化檢測時， 係用酵素催化流體中的待測物質與化學試劑，進而產生出訊號以供偵測。所 以要進行生化檢測，為了進行生化檢驗，第一流道U的硝化纖維層1121與 U31中的反應材料會包含有酵素與化學試劑。另一方面，若要檢測檢體中 的某些蛋白質’例如：胎兒蛋白（-fetoprotein)是否存在，則是利用具有 專一性之抗體，與待測蛋白質進行專一性結合，再利用其他化學試劑與已結 合上待測蛋白質的抗體進行反應，發出可供偵測的訊號。所以供免疫檢測的201013183 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention is directed to a type of inspection film, which is a test piece for turning over fluid detection. [Prior Art] In the conventional technique of performing biochemical detection and immunodetection using a fluid detecting test piece, the fluid detecting test piece is designed to have a nuclear or texture and a sulfur-hydrophobic treatment on the substrate wire, and the flow path is stomped. Conjoined water (4), and the fluid is riding on the Yang egg or the composition of the high-level composition, so when the fluid to be tested flows, it will remain on the flow path, making the fluid to be tested unable to fully react, so - come Not only causes waste of the fluid, but is more likely to cause errors in the most test results. In addition, the fluid detecting test piece of the prior art can be designed with a micro-flow channel structure, and utilizes the capillary phenomenon generated by the micro-flow channel structure to passively transfer the fluid through the flow channel to the reaction detecting area; The method is to give a driving force to the fluid by using a pressurized or vacuum negative pressure when injecting the fluid to be tested, or to design one or more micro-actuator or valve in the flow channel to make the fluid It can actively and sequentially pass through the flow path to reach the reaction detection area. However, in either of the above modes, the fluid to be tested is often injected or wound into a bubble of a different size to cause the flow path to clog, causing an error in actual measurement, or even causing the test to fail, and the micro-actuator or micro-actuator or The addition of valve increases the overall design difficulty and test piece cost. Moreover, the test strips of the prior art use a die casting, an injection molding or an imprint to make a flow path or a micro flow path structure on the substrate, so it is necessary to use polyethylene (PE), Higher-priced plastic polymers such as polyethylene (PVC) or polypropylene (PP) are used as materials and the molds are worn faster, which in turn leads to an increase in the overall cost of the test piece. In addition, different reaction materials or reagents are required due to the different reactions carried out. Conventional test strips are often designed to have only a single reagent or reactive material, so they are only suitable for single reactions and multiple tests at the same time in 201013183 samples. If it is to be different, this will cause inconvenience in use and a single type of test during testing. If it is not for single-test, it will cost different test strips. [Summary of the Invention] The domain service is based on the fact that the _ provides a kind of two-in-one-touch system can be used for both biochemical detection and immunization. Mainly comprising a substrate, the substrate is recessed downwardly from the upper surface thereof for the first channel of the biochemical detection and the second channel for the immunoassay, and the first channel and the second channel respectively comprise the first fluid sequentially connected The zone, the second fluid zone and the third fluid zone, the first fluid zone is provided for fluid injection. The integrated fluid detecting test piece is characterized in that each of the second fluid zone and the second fluid zone of the first flow channel and the second flow channel has a fiber layer, and the layer contains a hollow mesh structure. 'The second fluid zone is for fluid transfer and the third fluid zone is for fluid reaction. Further, the average thickness of the nitrocellulose layer in the first fluid region is not as large as the thickness of the ternary layer. A reactive material is formed in the hollow network configuration of the confirmed fiber layer. In addition, the substrate has longitudinal draw lines such that the third fluid zone of the first flow path and the second flow path are simultaneously located on the longitudinal axis. Therefore, the main object of the present invention is to provide a two-in-one fluid test strip capable of simultaneously performing biochemical and immunological tests on a single sample. Another object of the present invention is to provide a two-in-one fluid detecting test piece which has a nitrocellulose layer which can absorb liquid, thereby avoiding liquid residue in the flow path. Another object of the present invention is to provide a two-in-one fluid detecting test piece having a liquid-reducing fiber layer; since the liquid amount of the deuterated fiber per unit volume is constant, it can be nitrated through the setting substrate. The volume of the fibrous layer provides a quantitative detection of the fluid to be tested. Another object of the present invention is to provide a two-in-one fluid detecting test piece having a hollow mesh structure of a nitrocellulose layer, and the bubbles in the fluid are destroyed due to the fluid flowing through the hollow network configuration, so that it can be eliminated. The larger bubbles 'avoid the occurrence of bubbles blocking the flow path in the micro-channel technology, which in turn affects the quantitative analysis results. 201013183 [Embodiment] Since the present invention discloses a two-in-one fluid detecting test piece, the physical chemistry principle and/or the liquid-liquid coating technique used by the present invention have been known to those having ordinary knowledge in the related art, and therefore, the following Long made a full description. At the same time, the following diagrams of the texts, the indications related to the characteristics of the invention, are not required to be completely drawn according to the actual situation. Please refer to FIG. 1 for a preferred embodiment of the present invention, which is a two-in-one fluid detection test piece, which can be used for both biochemical detection and immunodetection. The two-in-one fluid detecting test piece 丨 mainly includes a substrate 10 and a support member 19. The substrate tube is recessed downward from its upper surface 100 with a first flow path 11 for biochemical detection and a second flow path 12 for immunodetection. The first flow path u includes a first fluid zone m, a second fluid zone 112 and a third fluid zone 113 which are sequentially connected. The second flow path 12 includes a first fluid zone 121, a second fluid zone 122, and a third fluid zone 123 that are sequentially connected. The first fluid zone 111 of the first flow path u and the first fluid zone 121 of the second flow path 12 communicate with each other for fluid injection. When the fluids are injected into the first fluid regions 11丨 and 12i that are connected to each other, respectively, the first flow channel u and the second flow flow are respectively transmitted via the first flow channel u and the second fluid regions 112 and 122 of the first flow channel 12, respectively. The third fluid zone 113 and 123 of the track 12; and the fluid flowing to the first flow channel η, when flowing to the first fluid zone 113, the component to be tested in the fluid will biochemically react here to generate a signal for debt Speculation. Similarly, the fluid flowing to the second flow path 12, when flowing to its third fluid zone 123, the component to be tested in the fluid will react there to generate a signal for detection. In a preferred embodiment, the substrate 10 is a biocompatible material.凊Continuing to refer to Fig. 2' is a plan view of the two-in-one fluid detecting test piece of the present invention. For ease of detection, the third fluid zone 113 of the first flow path u and the third fluid zone 123 of the second flow path 12 on the two-in-one fluid detecting test strip 1 are disposed on the same longitudinal axis of the substrate 1〇. 14 on. In this way, the detector coupled with the detection can detect the reaction of the third fluid region 113 of the first flow channel 11 and the third fluid region 123 of the second flow channel 12 as long as it moves on the same longitudinal axis 14. Signal. Please continue to refer to Figure 3, which is a cross-sectional view of the first flow path U along the eight-eighth line in Figure 1. At 7 201013183, at the bottom of the second fluid zone 112 and the third fluid zone ii3 of the first flow path 11, nitrocellulose layers 1121 and 1131 of a hollow network configuration are respectively formed. The average thickness Da of the nitrocellulose layer 1121 of the second fluid region 112 of the first flow channel is smaller than the thickness Db of the nitration fiber layer 1131 of the third fluid region 113 of the first flow channel u. Further, in the hollow network configuration of the nitrocellulose layers 1121 and 1131, the composition of the reaction material containing the reaction material is related to the type of the component to be tested contained in the fluid. Further, since the nitrocellulose layers 1121 and 1131 have a porous hollow network structure, the flow flowing in from the first fluid region 111 can be absorbed, and the components to be tested in the fluid and the reaction material present in the nitrocellulose layer 1131 can be absorbed. Carry out the reaction. ❹ Please continue to refer to Fig. 4, which is a cross-sectional view of the second flow path 12 along line BB in Fig. 1. The second flow path 12 is also the same as the first flow path 11, and nitrocellulose layers 1221 and 1231 of a hollow network configuration are formed at the bottoms of the second fluid region 122 and the third fluid region 123, respectively. Further, in the hollow network configuration of the nitrocellulose layers 1221 and 1231, 'the same as the nitrocellulose layers 1121 and 1131 in the first flow path, both of which contain a reactive material, and also have a porous hollow network structure, so The fluid flowing in from the first fluid zone 121 is absorbed and the component to be tested in the fluid reacts with the reactive material present in the nitrocellulose layer 1231. Since the first flow path 11 and the second flow path 12 have liquid nitrocellulose layers ι121, 113, 1221 and 1231, fluid can be prevented from remaining in the first flow path η and the second flow path 12. In addition, when the fluid flows through the nitrocellulose layers 1121, 1131, 1221, and 1231 having a hollow network configuration, the bubbles in the fluid are destroyed, so that the bubbles can be prevented from blocking the first flow path U and the second flow path 12. In addition, in order to reduce the influence of the capillary action between the flow path and the fluid, the first flow path and the second flow path proposed by the present invention are not so-called micro flow paths of the prior art, and are designed as shown in FIG. The second fluid region 112 width Wa of the first flow path 11, the third fluid region 113 width Wb of the first flow channel 11, the second fluid region 122 width Wc of the second flow channel 12, and the second flow channel 12 second The width Wd of the fluid region 123 is preferably at least 〇3 mm. In the production, the manner in which the nitrocellulose layers 1121, 1131, 1221, and 1231 are formed is as follows. First, a nitrocellulose powder is mixed with an 8 201013183 organic solvent containing an ester and a ketone to form a nitrocellulose solution; and the nitrocellulose solution is cast on the first flow path 11 The bottom of the second fluid zone 112 and the third fluid zone 113 and the second fluid zone 122 of the second flow channel 12 and the bottom of the third fluid zone 丨23. After drying, the bottom of the second fluid region 112 of the first flow channel n forms a layer of the bismuth fiber layer 112. The bottom of the third fluid region 113 of the first channel 11 forms a nitrocellulose layer 1131, and the second channel 12 The bottom of the second fluid region 122 forms a nitrocellulose layer 1221, and at the bottom of the third fluid region 123 of the second flow channel 12, a nitrocellulose layer 123h is formed for better pouring effect, the first flow channel u and the second flow path The surface roughness (Ra value) of the flow path 12 is preferably between 3 μm and 50 μm. A preferred volume ratio of the ruthenium fiber powder to the organic solvent containing the ester and the ketone is 1:9. Since the amount of liquid absorbed per unit volume of nitrocellulose is constant, the volume of the corresponding nitrocellulose solution can be derived from the volume of the fluid to be absorbed, and then cast, thereby fixing the volume of the liquid required for detection. And for micro-testing. The manner in which the reaction material is formed in the nitrocellulose layers 1121, 1131, 1221, and 1231 is as follows. After the nitrocellulose layers 1121, 1131, 1221 and 1231 are respectively dried and formed, the reaction solution containing the reaction material is injected into the air-dried or cold-dried (ly philizati〇n), and the reaction material is in the form of a powder. It remains in the nitrocellulose layers 1121, 1131, 1221 and 1231. The reaction material is formed in a manner other than the formation of the nitrocellulose layer and then the reaction material φ, and the reaction solution containing the reaction material may be added to the nitrocellulose powder and the ester-containing compound. (ester) and ketone (ketone) organic solvent composed of a stone solution fiber solution, after mixing is completed, 'the mixed solution is then cast in the second fluid zone 112 of the first flow channel 11 and the third The bottom of the fluid zone 113 and the bottom of the second fluid zone 122 and the third fluid zone 123 of the second flow channel 12 are subjected to an air drying or freeze drying process, and the nitrocellulose solution is formed into the nitrocellulose layers 1121, 1131, 1221 and 1231, And the reaction material is formed into a powder and remains in the nitrocellulose layers 1121, 1131, 1221, and 1231. As described above, the first flow path 11 is for biochemical detection, and the second flow path 12 is for immunodetection. Since the reactions required for biochemical and immunoassay are different, the composition of the reaction material in the nitrile 9 201013183 chemical fiber layers 1121 and 1131 formed in the first flow channel U, and the nitrocellulose layer formed in the second flow channel 12 The composition of the reaction materials in 1221 and 1231 is also different. For example, in the biochemical test, the enzyme is used to catalyze the test substance and the chemical reagent in the fluid, thereby generating a signal for detection. Therefore, biochemical tests are required. For biochemical tests, the reaction materials in the nitrocellulose layers 1121 and U31 of the first flow path U contain enzymes and chemical reagents. On the other hand, if it is necessary to detect the presence of certain proteins in the sample, such as the presence of a fetal protein (-fetoprotein), it is to use a specific antibody to specifically bind to the protein to be tested, and then to use other chemical reagents. The antibody that has been bound to the protein to be tested is reacted to emit a signal for detection. So for immunoassay
第一流道12，其硝化纖維層1221與1231中的反應材料則含有抗體及化學 等試劑。 此外，在較佳的實施狀態中，第二流道12上可增設第四流體區(未圖 示），其底部亦形成有硝化纖維層，藉以吸收多餘之流體。並且，如第4圖 所示，第二流道12的第二流體區122硝化纖維層1221的厚度De與第三流 體區123硝化纖維層1231的厚度Dd相同。 以上所述僅為本發明較佳實施例而已，並非用以限定本發明申請專利權 利；同時以上的描述對於熟之本技術領域之專門人士應可明瞭與實施，因此 其他未脫離本發明所揭示之精神下所完成的等效改變或修錦，均入 述之申請專利範圍。 … a 、 【圖式簡單說明】 第1圖，為本發明較佳實施例二合一流體檢測試片之示意圖。 第2圖，為本發明較佳實施例二合一流體檢測試片之俯視圖。 第3圖，為本發明較佳實施例二合一流體檢測試片第一流道剖面之示竟 圖0 第4圖，為本發明較佳實施例二合一流體檢測試片第二流道剖面之示意 201013183 【主要元件符號說明】 二合一流體檢測試片1 基板10 支撐件19 上表面100 第一流道11 第二流道12 縱向轴線14 第一流體區111、121 第二流體區112、122 第三流體區113、123 硝化纖維層 1121、1131、1221、1231 硝化纖維層1121平均厚度Da 硝化纖維層1131厚度Db 硝化纖維層1221平均厚度Dc 硝化纖維層1231厚度Dd 第二流體區112的寬度Wa 第三流體區113的寬度Wb 第二流體區122的寬度Wc 第三流體區123的寬度Wd 11In the first flow path 12, the reaction material in the nitrocellulose layers 1221 and 1231 contains reagents such as antibodies and chemicals. Further, in a preferred embodiment, a fourth fluid zone (not shown) may be added to the second flow path 12, and a nitrocellulose layer is formed at the bottom to absorb excess fluid. Further, as shown in Fig. 4, the thickness De of the nitrocellulose layer 1221 of the second fluid region 122 of the second flow path 12 is the same as the thickness Dd of the nitrocellulose layer 1231 of the third fluid region 123. The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention. The above description is to be understood by those skilled in the art, and thus the other embodiments are not disclosed. Equivalent changes or amendments made under the spirit of the company are included in the scope of the patent application. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic view showing a two-in-one fluid detecting test piece according to a preferred embodiment of the present invention. Fig. 2 is a plan view showing a two-in-one fluid detecting test piece according to a preferred embodiment of the present invention. FIG. 3 is a cross-sectional view showing a first flow path of a two-in-one fluid detecting test piece according to a preferred embodiment of the present invention. FIG. 4 is a schematic view showing a second flow path section of a two-in-one fluid detecting test piece according to a preferred embodiment of the present invention. 201013183 [Description of main component symbols] Two-in-one fluid detection test piece 1 Substrate 10 Support 19 Upper surface 100 First flow path 11 Second flow path 12 Longitudinal axis 14 First fluid zone 111, 121 Second fluid zone 112, 122 Three-fluid zone 113, 123 nitrocellulose layer 1121, 1131, 1221, 1231 nitrocellulose layer 1121 average thickness Da nitrocellulose layer 1131 thickness Db nitrocellulose layer 1221 average thickness Dc nitrocellulose layer 1231 thickness Dd width of second fluid zone 112 Wa Width Wb of third fluid zone 113 width Wc of second fluid zone 122 width Wd of third fluid zone 123 Wd 11