201035551 六、發明說明： 【發明所屬之技術領域】 本發明係關於-種檢測試片，特別是一種有關於生化檢測與免疫檢 ' 測所使用之流體檢測試片。 【先前技術】 以流體檢測試片進行生化檢測與免疫檢測的習知技術中，流體檢測 試片在其基板或底材上設計有凹陷的流道或微流道結構，並配合表面親 〇 疏水性處理，使待測流體可在流道中流動而不至溢出於流道外，但因流 道周圍並非吸水材t ’且待測流體多為含有如蛋自冑或是膽類等黏滞度 高之組成物，所以當待測流體流過後，會在流道周圍殘留，使得待測流 體無法完全反應，如此一來，不僅造成待測流體的浪費，更可能造成最 終測試結果的誤差。 此外，習知技術的流體檢測試片在流體傳送方面，可設計有微流道 結構’並係利用微流道結構產生的毛細現象，將流體經過流道被動傳送 至反應偵測區域；另一種方式則是在注入待測流體時即利用加壓或真空 ^ 負壓等方式’給予流體驅動力，又或於流道中設置一個或以上之微閥門 (micro-actuator or valve)等設計，使得流體可主動並依序通過流道，到達 反應偵測區域。但是無論是上述任一種方式，待測流體注入流道後常常 產生或捲入大小不一的氣泡使得流道阻塞，造成實際測量上之誤差，甚 至致使測試失敗’而微閥門(micro-actuator or valve)增設又增加整體設計 困難度與試片成本。 最後’習知技術的檢測試片，在製作上多使用模禱、射出成型、壓 印(imprint)或光刻電铸模造（LIGA、Lithographie GalVanoformung201035551 VI. Description of the Invention: [Technical Field of the Invention] The present invention relates to a test strip, and more particularly to a fluid test strip for use in biochemical detection and immunoassay. [Prior Art] In the conventional technique of performing biochemical detection and immunodetection using a fluid detecting test piece, the fluid detecting test piece is designed with a concave flow path or a micro flow path structure on the substrate or the substrate, and is matched with the surface relative hydrophobicity. Sexual treatment, so that the fluid to be tested can flow in the flow channel without overflowing outside the flow channel, but because the flow path is not around the water absorbing material t 'and the fluid to be tested is mostly containing such as egg self-tanning or bile The composition, so when the fluid to be tested flows, it will remain around the flow channel, so that the fluid to be tested cannot be completely reacted, which not only causes waste of the fluid to be tested, but is more likely to cause errors in the final test result. In addition, the fluid detecting test piece of the prior art can be designed with a microchannel structure in fluid transfer, and utilizes the capillary phenomenon generated by the microchannel structure to passively transfer the fluid through the flow path to the reaction detecting area; The method is to give a fluid driving force by using a pressure or a vacuum or a negative pressure when injecting a fluid to be tested, or to design one or more micro-actuator or valves in the flow channel to make the fluid It can actively and sequentially pass through the flow path to reach the reaction detection area. However, in either of the above manners, the fluid to be tested is often injected or entangled in the flow path to cause the flow path to be blocked, causing an error in the actual measurement, and even causing the test to fail, and the micro-actuator or valve The addition of additional design increases the difficulty of design and the cost of test strips. Finally, the test strips of the prior art are used in the production of mold, injection molding, imprinting or photolithography (LIGA, Lithographie GalVanoformung).
Abformung、Lithography Electroforming Micro Molding)的方式在基板上 做出流道或微流道結構，而這種方式需要高精度的模具，但模具非常容 201035551 易耗損，且此種製程對於模具耗損所造成誤差值的容忍度較低，因此使 得造成試片整體的生產成本大量增加。 因此，亟需提出一種流體檢測試片，以解決流體在流道上殘留，以 及試片製作成本過高的問題。 【發明内容】 為克服上述缺點，本發明提供一種流體檢測試片，主要包含有基板 及流道結構。絲具有平硫之第—表面，且親結構係設圖案形 成於該基板n Φ上，使得贿輯之表面高度不低於基板之第一 表面高度。流道結構具有中空網狀構型，且流道結構之親水性高於基板 第-表面之親雜。此外，频檢測刻進—步可包含反赌料形成於 中空網狀構型中。 因此’本發明之主要目的，係提供一種流體檢測試片，因流道結構 具有中空結構，故可敎雜前於流道周圍。 本發明之另—目的’係提供一種流體檢測試片，具有親水性之中空 網狀構型，由於單位體積財空網狀構型吸收液體量係為定值，故可經 由設定流道的軸，*提供侧紐缺量檢測。 本發明之X-目的，係提供—種流雜職片，由於_流經流道 之中空網狀構型時，流體中的氣泡會被破壞，故可消彌較大的氣泡，並 可避免微流道技術中氣泡阻塞流道的狀況發生，進而影響定量分析结 果。 '° ，本發明之再-目的，係提供一種流體檢測試片，利用預設流道圖 案形=於基板之表面上，故不需使職鑄、射出成型、壓印或光刻電 、、的方式在基板上做出流道或微流道結構，可節省大量製造流體 檢測試片時所需的成本。 本發明之再-目的’係提供一種流體檢測試片之 低成本且量敬加鮮驗驗職r , 201035551 【實施方式】 ，由於本發明係揭露一種流體檢測試片，其中所利用物理、化學原理 谷液塗佈技術，已為相關技術領域具有通常知識者所能明瞭，故以下 ‘ 文巾之制，*再作完整描述。啊，以下文巾賴照之s式，係表達 與本發明特徵有關之示意，並未亦不需要依據實際情形完整繪製，合先 敘明。 a清參考第ΙΑ ® ’為本發明之第―實施例紐制試片的示意圖。 流體檢測試片1包含有基板1〇與流道結構u。基板1〇具有平面狀之 〇 第一表* 100，而流道結構11係以預設随12形成於基板10之第一 ，面100上’且流道結構11之表面高度不低於基板10之第-表面100 间度’此外’流道結構u具有中空網狀構型，其親水性高於基板川 第表面100之親水性。流道結構丨1可由平版印刷、凹版印刷、凸 版印刷、網版印刷（screen printing)、劃線(line marking)、喷墨 (mkjet/spray)、洗注或浸染(dipping)等方式，將高親水性溶液塗布於基 板之帛表面1GG上’其成分可為硝化纖維或玻璃纖維，經乾燥固 化後具有多孔性的中空網狀構型，可以吸收待測流體，如此即可避免 待;體殘留於流道周圍的問題。而當流體流經流道結構η時，流體 巾的氣泡會被破壞’可避免造成氣泡阻塞。另夕卜，在本發明中更進一 步包3有成對的平面電極I3,形成於基板10之第一表面1〇〇與流道結 構11之HX檢測待測流體之電化學反應。在較佳的實施狀態中， 基板10為生物相容(bi〇eompatibie)材料且亦可為撓性材質。 接著請參閱第1B圖’為第1A圖所示的流體檢測試片1AA沿線剖 面圖。硝化纖維溶液在乾燥固化過程中會因内聚力使得流道結構之橫 截面具有凸起於第-表面励之構型，且因流道結構u係形成於基板 10之第一表面100上，故流道結構表面11〇與基板1〇之第一表面1〇〇 具有高度差h，亦即流道結構表面11〇高度不低於基板1〇之第一表面 100高度。再者，在流道結構u的中空網狀構型中包含有反應材料， 201035551 反應材料的組成係與流體中所含有的待測成份的種類有關。 用於形成流道結構11的溶液，其形成方式如下所述。先將硝化纖 維粉末（nitrocellulose powder)與含有醋類（ester)和綱類（ket〇ne) * 的有機溶劑混合後形成混合溶液，或將玻璃纖維溶於特定溶劑中形成混 合溶液，再將混合溶液依預設圖案塗布於基板10之第—表面上1〇〇， 經乾燥後，所形成之流道結構11具有中空網狀構型，具有吸液能力。 故當待測流體注入流體檢測試片1後，流道結構11即會已其中空網狀 結構所具有的吸液能力，將待測流體傳送至反應區域（未圖示）進行反 ❹ 應。 此外，由於本實施例中所使用的流道結構11全為中空網狀構型， 其單位體積的吸收液體量係為定值。當應用於定量檢測時，可由欲吸收 之待測流體的體積推算出對應的溶液體積；如此可以固定檢測所需液體 之體積量，並適用於微量檢測。 反應材料形成於流道結構11中空網狀構型的較佳方式則如下所 述。待上述混合溶液塗布於基板10上並乾燥形成流道結構u後，將含 有反應材料的反應溶液注入流道結構11，經過風乾或是冷凍乾燥 (lyophilization)後，反應材料則會以粉末狀的形式留存在流道結構u U 中。 由於待測成份不同，檢測所需進行之反應亦有所差異；進而依反應 種類的不同，產生出各種不同的訊號。例如進行生化檢測時，係用酵素 催化流體中的待測物質與冷光化學試劑，進而產生出特定波長的光訊號 以供偵測；或是利用特定酵素與待測物質進行反應（例如使用過氧化氫 腾來檢測血糖）’使其產生電流或電位的改變，藉以進行電化學的檢測。 所以要進行生化檢測，反應材料則會包含酵素及相對應的化學試劑。另 一方面，若要檢測檢體中的某些蛋白質一例如甲型胎兒蛋白 U-fetopmtein)或白蛋白（Aibumin)—是否存在，則是利用具有專— 201035551 人之禮與待概白質進行專一性結合，再利用其他化學試劑與已結 ^蛋白質的抗體進行反應’發出可供谓測的訊號。因此要進行免 紐、貞彳反雜料t騎包含有化學及抗體等免疫糊。故，本發明所 ， 提供之流體檢測試片，可躲各種生物檢體（如尿液、錢等流體）中 之各項待測成份的檢測。 明參考第1C圖’為本發明第-較佳實施例流體檢測試片之另一示 意圖。流道結構u可進-步包含有至少_個的分支流道ui及擴大區 域112。不同流體（例如：待測流體或反應所需試劑）可各自流經分支 〇 ",L道111 ’並在擴大區域112中充分混和。其中擴大區域112可為圓孤 形、矩形及島形等形狀，可使流道中的待測流體與反應材料有足夠的反 應時間，讓檢測結果更加準確。 另外，如第1D圖中所示，基板1〇之第一表面1〇〇上尚可形成堆 疊流道113，其中包含第-流道結構lm、第二流道結構1132及第三 流道節結構II33’堆疊流道⑴中之各流道結構可分別為相同流道圖案 所形成’或不同流道圖案所形成，依所需進行的測試種類及待測流體種 類而定。 本發明除和供上數第一實施例流體檢測試片之外，亦提供數種流 〇 體檢測試片的製造方法，如下列第二及第三較佳實施例中所述。 另外’請參考S2 ® ’林發明之第二較佳實補紐檢測試片的 製造方法流程圖，而本實施例中所提及之流體檢測試片的結構特徵如上 述第一較佳實施例中所述，且各元件編號請參考第1A圖至第1C圖， 以下不再重複贅述。流體檢測試片的製造方法主要包含下列步驟： 步驟21 .首先提供基板1 ’基板1具有平面狀的第一表面1〇〇。 步驟22 :提供一溶液，將溶液以塗覆方式沿預設圖案12形成於第 一表面100上。 步驟23 :乾燥塗布後的溶液，藉以形成具有預設圖案a之流道結 201035551 構1卜乾燥後的流道結構u之表面高度與基板1〇間有高度差h。在本 步驟中，溶液塗覆的方式可為平版印刷、凹版印刷、凸版印刷、網版印 — 刷(screen Printing)、劃線dine marking)、喷墨(inkjet/spray)。 ' 本發明第三較佳實施例為流體檢測試片的另-種製作方法，請參考 第3A圖及第3B圖。第3A圖，為本發明第三較佳實施例流體檢測試片 的製造方法流程圖；第3B圖則為依本實施例的的製造方法，在製造過 程中流體檢測試片的結構示意圖。 本實施例所提供的缝細抑的製造綠主要包含有下列步驟： 〇 步驟31 :首先提供基板30，基板30具有平面狀之第一表面3〇〇。 步驟32 ·提供遮帶33，遮帶33包含有鏤空之預設圖案32。 步驟33 .將遮帶33暫時性黏合於基板3〇上。 步驟34 :提供-雜’將溶液沾猶空之職圖案％。 步驟35 :乾燥沾滿預設圖案32上的溶液。 步驟36 :移除遮帶33，藉以形成具有預賴案32之流道結構心 乾燥成形後的流道輯31之表面之高度，不低於基板3()之第一表面 3⑻高度，且流道結構31具有中空網狀構型。而流道結構31的親水性 雨於基板30第一表面300之親水性。 ◎ 步驟37 :提供反應材料’使其形成於流道結構31的中空網狀構型 之中。 上述實施例之流道結構31形成方式、溶液之材質及反應材料形成 於中空網狀構型之方法皆與第-較佳實施财所述相同，在此不再加以 說明。 以上所述僅為本發明較佳實施例而已，並非用以限定本發明申請 專利權利；同日扣上_蘭於熟之本技術倾之和人士應可明瞭 與實施’鼠其他未齡本發明簡私精神下職較改變或 201035551 修飾，均應包含於下述之申請專利範圍。 【圖式簡單說明】 . 第1A圖’本發明之第一實施例流體檢測試片的示意圖。 第1B圖，第ία圖所示的流體檢測試片1 AA沿線剖面圖。 第1C圖’本發明第一較佳實施例流體檢測試片之另一示意圖。 第1D圖’本發明第一較佳實施例流體檢測試片之再一示意圖。 第2圖’本發明之第二較佳實施例流體檢測試片製造方法流程圖。 第3A圖’本發明第三較佳實施例流體檢測試片的製造方法流程圖。 〇 第3B圖’本發明第三較佳實施例流體檢測試片之示意圖。 【主要元件符號說明】 流體檢測試片 1 基板 10、20、30 第一表面 100、300 流道結構 11 ' 31 流道結構表面 110 預設圖案 12、32 平面電極 13 分支流道 111 擴大區域 112 堆疊流道 113 第一流道結構 1131 第二流道結構 1132 第二流道結構 1133 遮帶 33 流道結構表面與第一表面高度差 h 9Abformung, Lithography Electroforming Micro Molding) makes a flow path or micro flow path structure on the substrate, and this method requires a high-precision mold, but the mold is very easy to wear in 201035551, and this process causes errors in mold wear. The tolerance of the value is low, thus causing a large increase in the production cost of the entire test piece. Therefore, it is urgent to propose a fluid detecting test piece to solve the problem that the fluid remains on the flow path and the production cost of the test piece is too high. SUMMARY OF THE INVENTION In order to overcome the above disadvantages, the present invention provides a fluid detecting test piece, which mainly comprises a substrate and a flow channel structure. The filament has a first surface of flat sulfur, and the pro-structure is patterned on the substrate n Φ such that the surface height of the bribe is not lower than the first surface height of the substrate. The flow channel structure has a hollow network configuration, and the hydrophilicity of the flow channel structure is higher than that of the first surface of the substrate. In addition, the frequency detection engraving step can include the formation of an inverse gambling material in the hollow mesh configuration. Therefore, the main object of the present invention is to provide a fluid detecting test piece which has a hollow structure and can be noisy before the flow path. Another object of the present invention is to provide a fluid detecting test piece having a hydrophilic hollow mesh configuration. Since the liquid volume per unit volume of the network structure is constant, the axis of the flow path can be set. , * Provide side blank missing detection. The X-purpose of the present invention provides a kind of flow-in-the-life film. Since the bubble in the fluid will be destroyed when flowing through the hollow network configuration of the flow channel, large bubbles can be eliminated and can be avoided. In the micro-channel technology, the condition of the bubble blocking channel occurs, which in turn affects the quantitative analysis results. '°, a further object of the present invention is to provide a fluid detecting test piece which is formed on the surface of the substrate by using a predetermined flow path pattern, so that it is not required to be cast, injection molded, embossed or lithographically charged, The way to make a flow path or micro-channel structure on the substrate saves a lot of the cost of manufacturing a fluid test strip. The re-purpose of the present invention provides a low-cost and high-quality test for fluid test strips. 201035551 [Embodiment] Since the present invention discloses a fluid test strip, the physical and chemical utilized therein The principle of the liquid coating technology has been known to those of ordinary skill in the related art, so the following description will be made. Ah, the following swatches are based on the expressions of the features of the present invention, and are not required to be completely drawn according to the actual situation, which is described first. a clear reference to the first ® ' is a schematic diagram of the first embodiment of the invention. The fluid detecting test piece 1 includes a substrate 1 and a flow path structure u. The substrate 1 〇 has a planar first surface * 100, and the flow channel structure 11 is formed on the first surface of the substrate 10 with a preset 12, and the surface height of the flow path structure 11 is not lower than the substrate 10 The first-surface 100 degree 'further' flow channel structure u has a hollow network configuration which is more hydrophilic than the first surface 100 of the substrate. The flow path structure 丨1 can be high by lithography, gravure printing, letterpress printing, screen printing, line marking, inkjet (mkjet/spray), laundering or dipping. The hydrophilic solution is coated on the surface of the substrate 1GG. The composition of the substrate can be nitrocellulose or glass fiber. After drying and solidification, it has a porous hollow network configuration, which can absorb the fluid to be tested, thus avoiding the residue; Problems around the runner. When the fluid flows through the flow path structure η, the bubbles of the fluid towel are destroyed' to avoid bubble blockage. Further, in the present invention, further, the package 3 has a pair of planar electrodes I3 formed on the first surface 1 of the substrate 10 and the HX of the flow path structure 11 to detect the electrochemical reaction of the fluid to be tested. In a preferred embodiment, the substrate 10 is a biocompatible material and may also be a flexible material. Next, referring to Fig. 1B' is a cross-sectional view of the fluid detecting test piece 1AA shown in Fig. 1A. During the drying and solidification process, the nitrocellulose solution has a cohesive force such that the cross section of the flow channel structure has a convex surface in the first surface excitation configuration, and the flow channel structure u is formed on the first surface 100 of the substrate 10, so the flow The track surface 11〇 has a height difference h from the first surface 1〇〇 of the substrate 1,, that is, the height of the channel structure surface 11〇 is not lower than the height of the first surface 100 of the substrate 1〇. Furthermore, the reaction material is contained in the hollow network configuration of the flow path structure u, and the composition of the reaction material of 201035551 is related to the type of the component to be tested contained in the fluid. The solution for forming the flow path structure 11 is formed as follows. First, a nitrocellulose powder is mixed with an organic solvent containing ester and ket* to form a mixed solution, or the glass fiber is dissolved in a specific solvent to form a mixed solution, which is then mixed. The solution is applied to the first surface of the substrate 10 in a predetermined pattern, and after drying, the formed channel structure 11 has a hollow network configuration and has a liquid absorbing ability. Therefore, when the fluid to be tested is injected into the fluid detecting test piece 1, the flow path structure 11 has the liquid absorbing ability of the hollow mesh structure, and the fluid to be tested is sent to the reaction area (not shown) for reverse reaction. Further, since the flow path structure 11 used in the present embodiment is all in a hollow mesh configuration, the amount of absorbed liquid per unit volume is constant. When applied to quantitative detection, the corresponding solution volume can be deduced from the volume of the fluid to be absorbed; this can fix the volume of the liquid required for detection and is suitable for micro-detection. A preferred mode of forming the reaction material in the hollow network configuration of the flow path structure 11 is as follows. After the mixed solution is applied onto the substrate 10 and dried to form the flow channel structure u, the reaction solution containing the reaction material is injected into the flow channel structure 11, and after air drying or lyophilization, the reaction material is powdery. The form remains in the flow path structure u U . Due to the different components to be tested, the reactions required for the detection are also different; and depending on the type of reaction, various signals are generated. For example, in the biochemical test, an enzyme is used to catalyze a test substance in a fluid and a luminescent chemical to generate a specific wavelength of light for detection; or to use a specific enzyme to react with a substance to be tested (for example, using peroxidation) Hydrogen to detect blood sugar) 'make it produce a change in current or potential for electrochemical detection. Therefore, for biochemical testing, the reaction material will contain enzymes and corresponding chemical reagents. On the other hand, if it is necessary to detect the presence of certain proteins in the sample, such as U-fetopmtein or Aibumin, it is to use the special - 201035551 person's gift and the white matter to be specific. Sexual combination, and then use other chemical reagents to react with antibodies that have been blocked. Therefore, it is necessary to carry out the immune paste, which contains chemical and antibody, etc. Therefore, the fluid detecting test piece provided by the invention can hide the detection of each component to be tested in various biological samples (such as urine, money and the like). Referring to Fig. 1C, there is shown another schematic view of a fluid detecting test piece according to a first preferred embodiment of the present invention. The flow path structure u may further include at least one branch flow path ui and an enlarged area 112. Different fluids (e.g., the fluid to be tested or the reagent required for the reaction) may each flow through the branch quot ", the L channel 111' and be thoroughly mixed in the enlarged region 112. The enlarged region 112 may have a shape such as a circular shape, a rectangle, and an island shape, so that the fluid to be tested in the flow channel and the reaction material have sufficient reaction time, so that the detection result is more accurate. In addition, as shown in FIG. 1D, the stacking flow path 113 may be formed on the first surface 1 of the substrate 1 , including the first channel structure lm, the second channel structure 1132, and the third channel section. Each of the flow channel structures in the structure II33' stacking flow channel (1) may be formed by the same flow channel pattern or different flow channel patterns, depending on the type of test to be performed and the type of fluid to be tested. The present invention provides, in addition to the fluid detecting test piece of the first embodiment, a method of manufacturing a plurality of fluid detecting test pieces, as described in the following second and third preferred embodiments. In addition, please refer to the flow chart of the manufacturing method of the second preferred solid test strip of the S2 ® 'Lin invention, and the structural features of the fluid detecting test piece mentioned in the embodiment are as in the first preferred embodiment described above. For the description of each component number, refer to FIG. 1A to FIG. 1C, and the detailed description thereof will not be repeated below. The manufacturing method of the fluid detecting test piece mainly comprises the following steps: Step 21. First, the substrate 1 'the substrate 1 has a planar first surface 1 〇〇. Step 22: A solution is provided, and the solution is formed on the first surface 100 along the predetermined pattern 12 in a coating manner. Step 23: Drying the coated solution to form a channel junction having a predetermined pattern a. 201035551 The surface height of the dried channel structure u has a height difference h from the substrate 1〇. In this step, the solution coating may be by lithography, gravure printing, letterpress printing, screen printing, dine marking, inkjet/spray. The third preferred embodiment of the present invention is another method for producing a fluid detecting test piece, please refer to Figs. 3A and 3B. Fig. 3A is a flow chart showing a method of manufacturing a fluid detecting test piece according to a third preferred embodiment of the present invention; and Fig. 3B is a view showing the structure of the fluid detecting test piece in the manufacturing process according to the manufacturing method of the present embodiment. The manufacturing process of the slit fineness provided in this embodiment mainly comprises the following steps: 〇 Step 31: First, a substrate 30 is provided, which has a planar first surface 3〇〇. Step 32 - A mask 33 is provided, and the mask 33 includes a predetermined pattern 32 that is hollowed out. Step 33. Temporarily adhere the mask 33 to the substrate 3〇. Step 34: Provide - Miscellaneous. Step 35: Drying the solution on the predetermined pattern 32. Step 36: removing the mask 33, thereby forming the height of the surface of the runner 31 after the drying of the runner structure having the pre-requisite 32, not lower than the height of the first surface 3 (8) of the substrate 3, and flowing The track structure 31 has a hollow mesh configuration. The hydrophilicity of the runner structure 31 is hydrophilic to the hydrophilicity of the first surface 300 of the substrate 30. ◎ Step 37: Providing a reaction material 'to be formed in the hollow network configuration of the flow path structure 31. The method of forming the flow path structure 31 of the above embodiment, the material of the solution, and the method of forming the reaction material in the hollow mesh configuration are the same as those described in the first preferred embodiment, and will not be described here. The above description is only for the preferred embodiment of the present invention, and is not intended to limit the patent right of the present invention; on the same day, the person who is attached to the technology of the blue is familiar with the implementation of the mouse. The private spirit of the post-employment change or the 201035551 modification shall be included in the scope of the patent application below. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1A is a schematic view showing a fluid detecting test piece according to a first embodiment of the present invention. Fig. 1B is a cross-sectional view of the fluid detecting test piece 1 AA shown in Fig. 1A. 1C is another schematic view of a fluid detecting test piece according to a first preferred embodiment of the present invention. Fig. 1D is a schematic view showing the fluid detecting test piece of the first preferred embodiment of the present invention. Fig. 2 is a flow chart showing a method of manufacturing a fluid detecting test piece according to a second preferred embodiment of the present invention. Fig. 3A is a flow chart showing a method of manufacturing a fluid detecting test piece according to a third preferred embodiment of the present invention. 〇 Figure 3B is a schematic view of a fluid detecting test piece according to a third preferred embodiment of the present invention. [Main component symbol description] Fluid detecting test piece 1 Substrate 10, 20, 30 First surface 100, 300 Flow path structure 11 ' 31 Flow path structure surface 110 Preset pattern 12, 32 Planar electrode 13 Branch flow path 111 Enlarged area 112 Stacking flow path 113 First flow path structure 1131 Second flow path structure 1132 Second flow path structure 1133 Masking 33 Flow path structure surface and first surface height difference h 9