CN102086474A - 多重基因拷贝数检测试剂盒和方法 - Google Patents
多重基因拷贝数检测试剂盒和方法 Download PDFInfo
- Publication number
- CN102086474A CN102086474A CN2010101805512A CN201010180551A CN102086474A CN 102086474 A CN102086474 A CN 102086474A CN 2010101805512 A CN2010101805512 A CN 2010101805512A CN 201010180551 A CN201010180551 A CN 201010180551A CN 102086474 A CN102086474 A CN 102086474A
- Authority
- CN
- China
- Prior art keywords
- gene
- copy number
- internal reference
- dna
- amplified production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 155
- 238000000034 method Methods 0.000 title abstract description 19
- 239000012634 fragment Substances 0.000 claims abstract description 18
- 238000001514 detection method Methods 0.000 claims description 42
- 238000004519 manufacturing process Methods 0.000 claims description 27
- 238000006062 fragmentation reaction Methods 0.000 claims description 18
- 238000013467 fragmentation Methods 0.000 claims description 16
- 238000005251 capillar electrophoresis Methods 0.000 claims description 11
- 238000013461 design Methods 0.000 claims description 11
- 238000012408 PCR amplification Methods 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 230000008859 change Effects 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims description 7
- 230000037431 insertion Effects 0.000 claims description 7
- 238000012217 deletion Methods 0.000 claims description 6
- 230000037430 deletion Effects 0.000 claims description 6
- 230000008034 disappearance Effects 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 4
- 101500004783 Chlamydomonas moewusii Ceu clpP intein Proteins 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 238000013016 damping Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 108020004414 DNA Proteins 0.000 abstract description 71
- 230000003321 amplification Effects 0.000 abstract description 24
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 24
- 238000001962 electrophoresis Methods 0.000 abstract description 4
- 102000053602 DNA Human genes 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 108700026220 vif Genes Proteins 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 71
- 230000000875 corresponding effect Effects 0.000 description 16
- 238000005516 engineering process Methods 0.000 description 15
- 239000000047 product Substances 0.000 description 14
- 201000010374 Down Syndrome Diseases 0.000 description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- 206010044688 Trisomy 21 Diseases 0.000 description 7
- 230000002860 competitive effect Effects 0.000 description 7
- 238000007403 mPCR Methods 0.000 description 6
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000001819 mass spectrum Methods 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 238000011529 RT qPCR Methods 0.000 description 3
- 210000001766 X chromosome Anatomy 0.000 description 3
- 210000002593 Y chromosome Anatomy 0.000 description 3
- 238000012937 correction Methods 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000005477 standard model Effects 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 102100035475 Blood vessel epicardial substance Human genes 0.000 description 1
- 101150010856 CRT gene Proteins 0.000 description 1
- 102100027557 Calcipressin-1 Human genes 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 241001379910 Ephemera danica Species 0.000 description 1
- 101150007410 HDX gene Proteins 0.000 description 1
- 101001094636 Homo sapiens Blood vessel epicardial substance Proteins 0.000 description 1
- 101000580357 Homo sapiens Calcipressin-1 Proteins 0.000 description 1
- 101000608194 Homo sapiens Pyrin domain-containing protein 1 Proteins 0.000 description 1
- 101000595404 Homo sapiens Ribonucleases P/MRP protein subunit POP1 Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 101150025129 POP1 gene Proteins 0.000 description 1
- 101150084615 RCAN1 gene Proteins 0.000 description 1
- 108090000621 Ribonuclease P Proteins 0.000 description 1
- 102000004167 Ribonuclease P Human genes 0.000 description 1
- 101150059736 SRY gene Proteins 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 238000007630 basic procedure Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 108700010045 sry Genes Proteins 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (8)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201010180551 CN102086474B (zh) | 2010-05-24 | 2010-05-24 | 多重基因拷贝数检测试剂盒和方法 |
PCT/CN2011/074613 WO2011147309A1 (zh) | 2010-05-24 | 2011-05-24 | 多重基因拷贝数检测试剂盒和方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201010180551 CN102086474B (zh) | 2010-05-24 | 2010-05-24 | 多重基因拷贝数检测试剂盒和方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102086474A true CN102086474A (zh) | 2011-06-08 |
CN102086474B CN102086474B (zh) | 2013-08-28 |
Family
ID=44098479
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201010180551 Active CN102086474B (zh) | 2010-05-24 | 2010-05-24 | 多重基因拷贝数检测试剂盒和方法 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN102086474B (zh) |
WO (1) | WO2011147309A1 (zh) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102399900A (zh) * | 2011-12-16 | 2012-04-04 | 上海天昊生物科技有限公司 | 基因多态检测方法及试剂盒 |
CN106282356A (zh) * | 2016-08-30 | 2017-01-04 | 天津诺禾医学检验所有限公司 | 一种基于扩增子二代测序点突变检测的方法及装置 |
CN106929592A (zh) * | 2017-04-21 | 2017-07-07 | 上海杰傲奉生医学检验所有限公司 | X‑性连锁鱼鳞病相关sts基因拷贝数变异检测试剂盒 |
CN107326064A (zh) * | 2016-04-29 | 2017-11-07 | 天昊生物医药科技(苏州)有限公司 | 基因倒位突变检测方法 |
CN107475361A (zh) * | 2017-06-16 | 2017-12-15 | 上海兰卫医学检验所股份有限公司 | 一种检测临床疾病基因拷贝数改变的方法weca |
CN107529555A (zh) * | 2017-04-21 | 2018-01-02 | 上海杰傲奉生医学检验所有限公司 | 腓骨肌萎缩症相关pmp22基因拷贝数变异检测试剂盒 |
CN109609611A (zh) * | 2018-12-26 | 2019-04-12 | 上海优甲医疗科技有限公司 | 一种基于高通量测序技术的基因定量测序方法 |
CN111020023A (zh) * | 2019-09-11 | 2020-04-17 | 浙江中创生物医药有限公司 | 基因拷贝数定量分析 |
CN112852930A (zh) * | 2019-12-31 | 2021-05-28 | 湖北伯远合成生物科技有限公司 | 一种抗干扰pcr的方法 |
CN113957128A (zh) * | 2021-12-21 | 2022-01-21 | 迈杰转化医学研究(苏州)有限公司 | 一种核酸完整性评估试剂盒及评估方法 |
CN117344008A (zh) * | 2023-12-05 | 2024-01-05 | 北京华瀚基因科技有限公司 | 基于2-ΔΔCt法检测SMN1基因拷贝数的试剂盒 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110358815B (zh) * | 2019-07-18 | 2023-05-23 | 江苏宏微特斯医药科技有限公司 | 一种同时检测多个靶标核酸的方法及其试剂盒 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1693480A (zh) * | 2005-04-20 | 2005-11-09 | 浙江大学医学院附属妇产科医院 | 实时定量pcr技术检测人21号染色体数目的方法 |
WO2007081613A2 (en) * | 2005-11-28 | 2007-07-19 | Siemens Healthcare Diagnostics Inc. | A kinetic pcr assay for quantification of gene amplification on chromosome 17 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1557966B (zh) * | 2004-01-18 | 2010-05-05 | 上海市农业科学院生物技术研究中心 | 适合转基因棉花等外源基因检测的内标准基因及其应用 |
CN101613749A (zh) * | 2009-08-11 | 2009-12-30 | 中国人民解放军第二军医大学 | Papolb基因甲基化定量检测方法 |
-
2010
- 2010-05-24 CN CN 201010180551 patent/CN102086474B/zh active Active
-
2011
- 2011-05-24 WO PCT/CN2011/074613 patent/WO2011147309A1/zh active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1693480A (zh) * | 2005-04-20 | 2005-11-09 | 浙江大学医学院附属妇产科医院 | 实时定量pcr技术检测人21号染色体数目的方法 |
WO2007081613A2 (en) * | 2005-11-28 | 2007-07-19 | Siemens Healthcare Diagnostics Inc. | A kinetic pcr assay for quantification of gene amplification on chromosome 17 |
Non-Patent Citations (9)
Title |
---|
《BMC Genomics》 20070702 K.Iwao-Koizumi et al A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction 第8卷, 第206期 * |
《中华医学遗传学杂志》 19991226 杜琴等 运用荧光定量多重PCR技术检测RB1基因突变 386-389 第16卷, 第06期 * |
《中国临床康复》 20061115 杨渝等 毛细管电泳定量聚合酶链反应法对杜氏/贝氏进行性肌营养不良携带者的评估价值 101-103 第10卷, 第42期 * |
《湖南医科大学学报》 20030430 文曙等 荧光多重PCR对alpha地中海贫血的基因诊断 123-126 第28卷, 第02期 * |
《遗传》 20070815 高斌等 多重实时荧光PCR相对定量法快速诊断唐氏综合征 934-938 第29卷, 第08期 * |
K.IWAO-KOIZUMI ET AL: "A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction", 《BMC GENOMICS》 * |
K.IWAO-KOIZUMI ET AL: "A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction", 《BMC GENOMICS》, vol. 8, no. 206, 2 July 2007 (2007-07-02) * |
L.ZENTILIN AND M.GIACCA: "Competitive PCR for precise nucleic acid quantification", 《NATURE PROTOCOLS》 * |
L.ZENTILIN AND M.GIACCA: "Competitive PCR for precise nucleic acid quantification", 《NATURE PROTOCOLS》, vol. 2, no. 9, 23 August 2007 (2007-08-23), XP055231861, DOI: doi:10.1038/nprot.2007.299 * |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102399900A (zh) * | 2011-12-16 | 2012-04-04 | 上海天昊生物科技有限公司 | 基因多态检测方法及试剂盒 |
CN102399900B (zh) * | 2011-12-16 | 2013-10-30 | 上海天昊生物科技有限公司 | 基因多态检测方法及试剂盒 |
CN107326064A (zh) * | 2016-04-29 | 2017-11-07 | 天昊生物医药科技(苏州)有限公司 | 基因倒位突变检测方法 |
CN106282356A (zh) * | 2016-08-30 | 2017-01-04 | 天津诺禾医学检验所有限公司 | 一种基于扩增子二代测序点突变检测的方法及装置 |
CN106282356B (zh) * | 2016-08-30 | 2019-11-26 | 天津诺禾医学检验所有限公司 | 一种基于扩增子二代测序点突变检测的方法及装置 |
CN107529555A (zh) * | 2017-04-21 | 2018-01-02 | 上海杰傲奉生医学检验所有限公司 | 腓骨肌萎缩症相关pmp22基因拷贝数变异检测试剂盒 |
CN106929592A (zh) * | 2017-04-21 | 2017-07-07 | 上海杰傲奉生医学检验所有限公司 | X‑性连锁鱼鳞病相关sts基因拷贝数变异检测试剂盒 |
CN107475361A (zh) * | 2017-06-16 | 2017-12-15 | 上海兰卫医学检验所股份有限公司 | 一种检测临床疾病基因拷贝数改变的方法weca |
CN109609611A (zh) * | 2018-12-26 | 2019-04-12 | 上海优甲医疗科技有限公司 | 一种基于高通量测序技术的基因定量测序方法 |
CN111020023A (zh) * | 2019-09-11 | 2020-04-17 | 浙江中创生物医药有限公司 | 基因拷贝数定量分析 |
CN112852930A (zh) * | 2019-12-31 | 2021-05-28 | 湖北伯远合成生物科技有限公司 | 一种抗干扰pcr的方法 |
CN113957128A (zh) * | 2021-12-21 | 2022-01-21 | 迈杰转化医学研究(苏州)有限公司 | 一种核酸完整性评估试剂盒及评估方法 |
CN117344008A (zh) * | 2023-12-05 | 2024-01-05 | 北京华瀚基因科技有限公司 | 基于2-ΔΔCt法检测SMN1基因拷贝数的试剂盒 |
CN117344008B (zh) * | 2023-12-05 | 2024-03-08 | 北京华瀚基因科技有限公司 | 基于2-ΔΔCt法检测SMN1基因拷贝数的试剂盒 |
Also Published As
Publication number | Publication date |
---|---|
CN102086474B (zh) | 2013-08-28 |
WO2011147309A1 (zh) | 2011-12-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102086474B (zh) | 多重基因拷贝数检测试剂盒和方法 | |
CN102625850B (zh) | 多重核酸检测方法和系统 | |
JP2020072674A (ja) | 核酸の標準化された配列決定のための方法およびその使用 | |
CN101921834B (zh) | 一种abo血型基因分型的pcr-sbt方法及试剂 | |
KR20130016010A (ko) | 보습 피부 타입 유전자 다형성 마커 및 이의 용도 | |
CN1981052A (zh) | 检测染色体病 | |
CN101845520B (zh) | 一种hpa等位基因分型检测试剂盒 | |
Pestoni et al. | The use of the STRs HUMTH01, HUMVWA31/A, HUMF13A1, HUMFES/FPS, HUMLPL in forensic application: validation studies and population data for Galicia (NW Spain) | |
CN103923973A (zh) | 一种基于数字pcr平台检测基因缺失突变的方法和试剂盒 | |
CN113614246A (zh) | 用于鉴别肿瘤模型的方法和组合物 | |
CN106701978A (zh) | 一种人类微卫星不稳定性msi检测扩增引物组合物及试剂盒 | |
CN106701987A (zh) | 人叶酸代谢相关的三个snp位点基因分型的pcr扩增系统和检测试剂盒 | |
CN105624296A (zh) | 基于arms荧光pcr法检测基因多态性的通用型荧光发夹引物 | |
CN110541033A (zh) | Egfr基因突变检测用组合物及检测方法 | |
CN110564861B (zh) | 人类Y染色体STR基因座和InDel位点的荧光标记复合扩增试剂盒及其应用 | |
Saelee et al. | Quantitative PCR–based method to assess cell-free DNA quality, adjust input mass, and improve next-generation sequencing assay performance | |
CN109097479A (zh) | Cyp2c19基因多态性检测试剂盒 | |
CN110846409A (zh) | 用于检测tnni3k基因突变的引物组合及其应用 | |
US20210292829A1 (en) | High throughput assays for detecting infectious diseases using capillary electrophoresis | |
CN105543370A (zh) | 新的综合性耳聋相关基因突变检测体系及试剂盒 | |
CN112342303A (zh) | 一种基于ngs的人类y染色体str和snp遗传标记联合检测体系及检测方法 | |
CN102399900B (zh) | 基因多态检测方法及试剂盒 | |
Crawford et al. | Reproducible gene expression measurement among multiple laboratories obtained in a blinded study using standardized RT (StaRT)-PCR | |
KR20200081318A (ko) | 멀티플렉스 시스템을 이용한 인간 객체의 y str 유전좌위 분석방법 및 이를 이용한 분석 키트 | |
CN101548010A (zh) | 测定多等位基因单倍型的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Kit and method for detecting copy number of multiple genes Effective date of registration: 20170908 Granted publication date: 20130828 Pledgee: Pudong Shanghai technology financing Company limited by guarantee Pledgor: SHANGHAI GENESKY BIO-TECH Co.,Ltd. Registration number: 2017310000050 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PP01 | Preservation of patent right | ||
PP01 | Preservation of patent right |
Effective date of registration: 20190613 Granted publication date: 20130828 |
|
PD01 | Discharge of preservation of patent |
Date of cancellation: 20190816 Granted publication date: 20130828 |
|
PD01 | Discharge of preservation of patent | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210208 Granted publication date: 20130828 Pledgee: Pudong Shanghai technology financing Company limited by guarantee Pledgor: SHANGHAI GENESKY BIO-TECH Co.,Ltd. Registration number: 2017310000050 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Multiple gene copy number detection kit and method Effective date of registration: 20210208 Granted publication date: 20130828 Pledgee: Pudong Shanghai technology financing Company limited by guarantee Pledgor: SHANGHAI GENESKY BIO-TECH Co.,Ltd. Registration number: Y2021310000006 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230816 Granted publication date: 20130828 Pledgee: Pudong Shanghai technology financing Company limited by guarantee Pledgor: SHANGHAI GENESKY BIO-TECH Co.,Ltd. Registration number: Y2021310000006 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |