CN101945997B - 产生γ-氨基丁酸的方法 - Google Patents

产生γ-氨基丁酸的方法 Download PDF

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Publication number
CN101945997B
CN101945997B CN200980105702.3A CN200980105702A CN101945997B CN 101945997 B CN101945997 B CN 101945997B CN 200980105702 A CN200980105702 A CN 200980105702A CN 101945997 B CN101945997 B CN 101945997B
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decarboxylase
gaba
glutamic
sequence
nucleic acid
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CN101945997A (zh
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O·策尔德尔
W·K·郑
C·克洛普罗格
A·赫罗尔德
H·施罗德
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BASF SE
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01015—Glutamate decarboxylase (4.1.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polyamides (AREA)
CN200980105702.3A 2008-02-21 2009-02-20 产生γ-氨基丁酸的方法 Expired - Fee Related CN101945997B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08151744.3 2008-02-21
EP08151744 2008-02-21
PCT/EP2009/001225 WO2009103547A1 (en) 2008-02-21 2009-02-20 Process for the production of gamma-aminobutyric acid

Related Child Applications (1)

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CN201410601958.6A Division CN104357431A (zh) 2008-02-21 2009-02-20 产生γ-氨基丁酸的方法

Publications (2)

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CN101945997A CN101945997A (zh) 2011-01-12
CN101945997B true CN101945997B (zh) 2014-12-03

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CN201410601958.6A Pending CN104357431A (zh) 2008-02-21 2009-02-20 产生γ-氨基丁酸的方法

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US (2) US8771998B2 (enExample)
EP (1) EP2247724A1 (enExample)
JP (2) JP2011512144A (enExample)
KR (1) KR20110004368A (enExample)
CN (2) CN101945997B (enExample)
WO (1) WO2009103547A1 (enExample)

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CN101945997B (zh) * 2008-02-21 2014-12-03 巴斯夫欧洲公司 产生γ-氨基丁酸的方法
EP2204443B1 (en) * 2008-12-23 2015-11-25 Basf Se Bacterial cells exhibiting formate dehydrogenase activity for the manufacture of suc-cinic acid
ES2559385T3 (es) * 2008-12-23 2016-02-11 Basf Se Células bacterianas que tienen una derivación de glioxilato para la fabricación de ácido succínico
CN102317438A (zh) 2009-02-16 2012-01-11 巴斯夫欧洲公司 琥珀酸的新型微生物生产者和琥珀酸的纯化
WO2011137369A1 (en) * 2010-04-29 2011-11-03 The Regents Of The University Of California Production of gamma-aminobutyric acid by recombinant microorganisms
US8728775B2 (en) * 2010-09-30 2014-05-20 Daesang Corporation Method for preparing 2-pyrrolidone using a microorganism containing glutamate decarboxylase
CN102154393A (zh) * 2011-01-18 2011-08-17 江南大学 一种γ-氨基丁酸的生产方法及其生产菌株
PE20141569A1 (es) 2011-11-11 2014-11-08 Ajinomoto Kk Metodo para producir una sustancia diana mediante fermentacion
CN102864190A (zh) * 2012-10-10 2013-01-09 山东金城钟化生物药业有限公司 γ-氨基丁酸的生产方法
CN103555779B (zh) * 2013-08-01 2015-08-19 江南大学 一种发酵生产γ-氨基丁酸的方法
CN103555647B (zh) * 2013-11-06 2015-05-27 南京工业大学 一种高产γ-氨基丁酸的重组谷氨酸棒杆菌及构建方法和应用
WO2015092599A1 (en) * 2013-12-17 2015-06-25 Basf Se Improved process for the production of gamma-aminobutyric acid (gaba)
WO2015092576A1 (en) * 2013-12-17 2015-06-25 Basf Se Improved process for the production of gamma-aminobutyric acid (gaba)
CN104099366B (zh) * 2014-07-11 2017-01-18 苏州凯祥生物科技有限公司 一种谷氨酸脱羧酶重组质粒及其构建方法和应用
CN105647912B (zh) * 2014-12-01 2025-09-19 清华大学 抗酸表达盒及其筛选方法
KR20160077272A (ko) 2014-12-22 2016-07-04 한국광기술원 Led를 이용한 안구 피로회복 장치
CN104558589A (zh) 2015-01-08 2015-04-29 华东理工大学 一种绿色尼龙聚丁内酰胺的制备方法
CN104830886B (zh) * 2015-05-07 2017-09-22 福建师范大学 产γ‑氨基丁酸的重组菌及其构建方法与应用
CN105296456B (zh) * 2015-11-23 2018-08-07 江南大学 一种pH稳定性提高的谷氨酸脱羧酶突变体及其应用
JP7113720B2 (ja) * 2018-10-19 2022-08-05 水澤化学工業株式会社 γ－アミノ酪酸捕集剤
CN111378611B (zh) * 2018-12-29 2022-02-22 杭州唯铂莱生物科技有限公司 一种谷氨酸脱羧酶重组菌及其构建方法和应用
CN110951664B (zh) * 2019-12-27 2021-09-24 江南大学 一种重组谷氨酸棒杆菌及其在生产2-吡咯烷酮中的应用
CN111635898B (zh) * 2020-06-17 2022-04-29 中国科学院天津工业生物技术研究所 谷氨酸脱羧酶突变体及其在制备γ-氨基丁酸中的应用
CN114752544B (zh) * 2022-06-16 2022-09-06 森瑞斯生物科技(深圳)有限公司 一步法生产γ-氨基丁酸的方法及其菌株构建
CN115927278B (zh) * 2022-12-23 2024-05-03 山西农业大学 一种与甘薯γ-氨基丁酸代谢相关的蛋白及其编码基因和应用
CN116286578B (zh) * 2023-03-06 2024-12-20 华南理工大学 一种能够合成gaba的酪丁酸梭菌基因工程菌及其构建方法与应用

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Publication number Publication date
US20140256005A1 (en) 2014-09-11
WO2009103547A1 (en) 2009-08-27
US20100324258A1 (en) 2010-12-23
EP2247724A1 (en) 2010-11-10
KR20110004368A (ko) 2011-01-13
CN101945997A (zh) 2011-01-12
CN104357431A (zh) 2015-02-18
JP2011512144A (ja) 2011-04-21
US8771998B2 (en) 2014-07-08
JP2015119707A (ja) 2015-07-02

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