CN101898907A - Compound microbial preparation and application thereof in microbial degradation of crop straw - Google Patents
Compound microbial preparation and application thereof in microbial degradation of crop straw Download PDFInfo
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- CN101898907A CN101898907A CN201010146397.7A CN201010146397A CN101898907A CN 101898907 A CN101898907 A CN 101898907A CN 201010146397 A CN201010146397 A CN 201010146397A CN 101898907 A CN101898907 A CN 101898907A
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Abstract
The invention relates to a compound microbial preparation and an application thereof. The compound microbial preparation of the invention is prepared from cellulose decomposition bacteria and surfactants, wherein the cellulose decomposition bacteria belong to trichoderma. The compound microbial preparation is added into fresh straw to increase the degradation efficiency of the straw by degrading cellulose, lignin and hemicellulose in the straw, thereby shortening the degradation time of the straw, returning the straw into the field and restoring the degraded soil.
Description
The method field
The present invention relates to a kind of complex microorganism preparations, be specifically related to a kind of complex micro organism fungicide of forming by cellulose-decomposing bacterium and tensio-active agent, also relate to the application of this complex microorganism preparations in the microbiological deterioration of crop material.
Background method
Strengthen Energy resources saving and ecological environmental protection, strengthen the capability of sustainable development, be related to the vital interests of the people and Chinese nation's survival and development.Along with countries in the world to the attention of the variation of global climate and the appearance of energy dilemma, various countries unite discusses the dirty needs that reduce discharging of joint, stalk all has big prospect at aspects such as energy, fertilizerization, feedizations under this background.
Stalk is a kind of biomass energy resource, is the fourth-largest energy that is only second to coal, oil and natural gas in the world today, is unique reproducible energy.But China's stalk resource 45%～47% is as rural area fuel, and 15% burns with the form of burning the grass on waste land, and has only seldom a part to cross abdomen field or direct returning to farmland.Straw directly burns not only contaminate environment, and causes the wasting of resources.
Utilizing the microbial fermentation degrading straw is the good approach of fertilizerization, but straw directly returning to field, there are a lot of particular problems, low as the straw utilization ratio, not perishable, influence following batch of sowing, easily emersion soil face wafts is etc. everywhere, also has report to show easier mashed root of crop and the disease of causing of straw directly returning to field.Stalk indirectly also the field technology be crop material to be piled corruption macerate also field, system back, the forms such as agent heap corruption that comprise that the stalk heap is rotten, high temperature nuclear reactor is rotten, stalk becomes thoroughly decomposed; Stalk is made base-material and is produced edible mushrooms, again with the waste residue fertile also field in field and natural pond etc. also.
These technology exist various particular problems and deficiency equally, and for example technology is immature, and efficient is not high, and bacterial classification is ungood etc.Therefore,, develop a circular economy, realize Sustainable development in order to repair the soil of degeneration, must a kind of efficient good microbiobacterial agent of development.
Summary of the invention
In order to overcome the problems of the prior art, the objective of the invention is to develop a kind of compound microbiobacterial agent, improve the degradation efficiency of stalk, promote straw-returning, to scheme to repair the soil of degenerating.
In order to achieve the above object, the present invention adopts following technical scheme:
The invention provides a kind of compound microbial preparation, is formulated by 1: 2～2: 1 volume ratio by cellulose-decomposing bacterium and tensio-active agent.
Wherein, described cellulose-decomposing bacterium belongs to wood mould (Trichoderma sp.) and belongs to filamentous fungus, this bacterial strain on March 11st, 2010 in China Committee for Culture Collection of Microorganisms's preservation, deposit number is CGMCC No.3662, prepare by the following method: stalk is rotted to approaching with soil or compost dipping, with alcohol to after carrying out surface sterilization near the stalk that rots, be cut into the long small segment of 1～3cm, the straw small segment that will partly become thoroughly decomposed changes over to sterilization metal tweezers and is equipped with in the 25mL 95% alcoholic acid flat board, taking-up changes new sterilization plate inboard over to after soaking 90s, treat that surperficial alcohol dries that (5～10min) backs change PDA solid medium (potato glucose agar medium over to the metal tweezers of sterilizing, finished product 32-34g, agar powder 8-10g) in the plate, seal plate with plastics film, plate is inverted cultivation 5～10d after putting cultivation 8d, at growth fungal colony edge picking fritter bacterium colony, change in the PDA solid medium plate and cultivate, if find growth assorted bacterium is arranged, the picking colony edge changes in the new plate that the PDA solid medium is housed and continues purifying, obtains cellulose-decomposing bacterium.
Wherein said PDA solid medium is: potato glucose agar medium, finished product 32-34g, agar powder 8-10g.
Described tensio-active agent can adopt synthetic tensio-active agent or bio-surfactant.Described synthetic tensio-active agent comprises cetyl trimethylammonium bromide, sodium laurylsulfonate, PEG6000, Sodium dodecylbenzene sulfonate, tween etc.Described bio-surfactant is meant the self-faced active substance that is produced by microorganism, plant or animal, and as glycolipid, saccharose lipid, lipopeptid or neutral quasi-grease derivative etc., for example pseudomonas aeruginosa produces the glycolipid that contains rhamnosyl.The effect that described tensio-active agent plays is the utilizability that has improved stalk.
In the preferred embodiment of the invention, used bio-surfactant is to have an active amphiprotic substance of certain surface by microorganisms, be the compost of soil sample below the various stalks heap of long-term accumulation and cattle farm, under the low temperature culture condition (5～10 ℃), through bacterium enrichment culture, blood agar line primary dcreening operation, the blue gel flat band method is sieved again and the Pseudomonas aeruginosa (Pseudomonas aeruginosa) that obtains produces, and belongs to sophorolipid.Described pseudomonas aeruginosa strains on March 11st, 2010 in China Committee for Culture Collection of Microorganisms's preservation, deposit number is CGMCC No.3661.
The preparation method of complex microorganism preparations of the present invention is to form by the suitable proportion mixed preparing separating the cellulose-decomposing bacterium and the tensio-active agent that obtain by described method.Described ratio is the volume ratio preparation by 1: 2～2: 1.
The present invention also provides the application of described complex microorganism preparations as straw degradative, is that complex micro organism fungicide is executed equably on the fresh straw surface, and the consumption of complex microorganism preparations is 4～5% of a fresh straw weight.This complex microorganism preparations has increased the degradation efficiency of stalk, shortens the straw degradative time, thereby promotes stalk to go back the field fast, is beneficial to repair the soil of degeneration.
Complex microorganism preparations and in the microbiological deterioration of crop material be applied as stalk fast also the field a kind of pattern is provided, improved the transformation efficiency that agricultural wastes are produced fertilizer, be beneficial to the reparation of degenerated soil, increased soil fertility.Through experimental test, complex microorganism preparations of the present invention imposes on the bright stalk, do not compare with the stalk contrast with adding complex microorganism preparations, soil moisture content exceeds 15～20%, and soil bacteria quantity exceeds 2～3 times, the quantity of soil phosphorus-solubilizing bacteria improves 40～44%, the soil nitrification number of bacteria improves 3～4 times, and crop root length improves 25～30%, and stem of plant minister degree improves 30～34%, crop weight improves 32～37%, and seedling stage, ground biomass improved 45～55%.
Description of drawings
Fig. 1 identifies figure for the infrared spectra of the bio-surfactant that the psychrotroph bacterial strain produces.
Cellulose-decomposing bacterium of the present invention belongs to wood mould (Trichoderma sp.) and belongs to filamentous fungus, this bacterial strain on March 11st, 2010 in China Committee for Culture Collection of Microorganisms's preservation, deposit number is CGMCC No.3662.Described Pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain on March 11st, 2010 in China Committee for Culture Collection of Microorganisms's preservation, deposit number is CGMCC No.3661
Embodiment
Be described in further detail the present invention below.
The invention provides a kind of compound microbial preparation, is formulated by 1: 2～2: 1 volume ratio by cellulose-decomposing bacterium and tensio-active agent.
Wherein, described cellulose-decomposing bacterium belongs to the Trichoderma filamentous fungus, this bacterial strain on March 11st, 2010 in China Committee for Culture Collection of Microorganisms's preservation, deposit number is CGMCC No.3662, prepare by the following method: stalk is rotted to approaching with soil or compost dipping, with alcohol to after carrying out surface sterilization near the stalk that rots, be cut into the long small segment of 1～3cm, the straw small segment that will partly become thoroughly decomposed changes over to sterilization metal tweezers and is equipped with in the 25mL 95% alcoholic acid flat board, taking-up changes new sterilization plate inboard over to after soaking 90s, treat that surperficial alcohol dries that (5～10min) backs change PDA solid medium (potato glucose agar medium over to the metal tweezers of sterilizing, finished product 32-34g, agar powder 8-10g) in the plate, seal plate with plastics film, plate is inverted cultivation 5～10d after putting cultivation 8d, at growth fungal colony edge picking fritter bacterium colony, change in the PDA solid medium plate and cultivate, if find growth assorted bacterium is arranged, the picking colony edge changes in the new plate that the PDA solid medium is housed and continues purifying, obtains cellulose-decomposing bacterium.
Described tensio-active agent can adopt synthetic tensio-active agent or bio-surfactant.Described synthetic tensio-active agent comprises cetyl trimethylammonium bromide, sodium laurylsulfonate, PEG6000, Sodium dodecylbenzene sulfonate, tween etc.Described bio-surfactant is meant the self-faced active substance that is produced by microorganism, plant or animal.The effect that described tensio-active agent plays is the utilizability that has improved stalk.
The invention provides a kind of bio-surfactant, be the compost of soil sample below the various stalks heap of long-term accumulation and cattle farm, under the low temperature culture condition (5～10 ℃), sieve again through bacterium enrichment culture, blood agar line primary dcreening operation, blue gel flat band method, filter out and have that surface-active bacterial strain produces, belong to sophorolipid.
The screening of bacterial strain
1. bacterium enrichment culture
Get the various concentration (10 of 5ml respectively
-2, 10
-3, 10
-4, 10
-5, 10
-6, 10
-7, 10
-8, 10
-9G/ml) soil supension is inoculated in the bacterium enrichment medium, and 1～2d is cultivated in 24 ℃ of concussions, and rotating speed 200r/min obtains pregnant solution.
Described bacterium enrichment medium (XF) is: vegetables oil 4g, (NH
4)
2SO
410g, KCl 1.1g, FeSO
47H
2O 2.5 * 10
-5G, KH
2PO
43.4g, K
2HPO
43H
2O 5g, MgSO
40.5g, EDTA1g, yeast extract 0.5g is settled to 1L with distilled water, and the pH value is 7.0.
2. blood agar substratum primary dcreening operation
Above-mentioned pregnant solution is streak culture on the blood agar substratum, to select the bigger bacterial strain of generation haemolysis circle and carry out separation and purification, single bacterium colony of separation and purification is connected on the slant medium, cultivates 1～2d for 30 ℃.
Described blood agar substratum (g/L) is: sheep blood 8～10%, and extractum carnis 0.3%, peptone 1%, yeast powder 0.01%, NaCl 0.5%, agar powder 1.7%, pH 6～7,115 ℃ of sterilization 15min.
Described slant medium: glucose 16g, peptone 4g, yeast extract 0.2g, agar 20g is settled to 1L with distilled water, and the pH value is 6.5.
3. the blue gel substratum sieves again
Get the various concentration (10 of 5ml respectively
-2,10
-3, 10
-4, 10
-5, 10
-6, 10
-7, 10
-8, 10
-9G/ml) soil supension is inoculated in aforementioned enrichment medium (XF), and 1～2d, rotating speed 200r/min are cultivated in 24 ℃ of concussions.The blue gel plate culture medium is coated in the pregnant solution dilution, the bacterial strain that produces blue circle around the choosing colony carries out separation and purification, single bacterium colony of separation and purification is connected on the aforementioned slant medium, cultivate 1～2d for 30 ℃, with the bacterial classification of slant culture transfer in seed culture medium cultivate 16h after, be inoculated in the shake-flask culture liquid 30 ℃ of shaking culture 2～3d, rotating speed 200r/min, thus described Pseudomonas aeruginosa obtained.
Described blue gel plate culture medium (g/L) is: extractum carnis 1, glucose 20, peptone 5, yeast extract paste 0.2, agar 18, cetyl trimethyl bromination 0.2, methylene blue 0.005.
Described seed culture medium is: peptone 10g, extractum carnis 3g, NaCl 5g, be settled to 1L with distilled water, the pH value is 7.2.
4. arranging butyrous measures
Above-mentioned isolated single strain is carried out liquid fermentation and culture, and (fermention medium is glucose 20g/L, yeast extract paste 1g/L, NH
4NO
32.5g/L, KH
2PO
41g/L, Na
2HPO
41g/L, MgSO
47H
2O 0.02g/L, inorganic salt solution 3mL/L, n-hexadecane 2mL/L, pH7.0, wherein inorganic salt solution is zinc sulfate 0.029g/L, calcium chloride 0.024g/L, copper sulfate 0.025g/L, sal epsom 0.017g/L), with its fermented liquid respectively under normal temperature (25 ℃) and low temperature environment (0～10 ℃) do oil extraction mensuration.The distilled water that promptly in a glass dish (D=9cm), adds 60mL, the n-dodecane that adds 8mL then, after treating that n-dodecane is uniformly distributed on the water surface, the fermented liquid that adds 1mL on its surface, if produce the oil extraction circle, then prove to have bio-surfactant in the fermented liquid, otherwise, lifeless matter tensio-active agent in this fermented liquid then proved.The oil extraction circle is big more, illustrates that oil extraction is good more.The bacterial strain of choosing oil extraction good (being that its fermented liquid oil extraction diameter is greater than 3cm) keeps, and proceeds next step the capillary determination test of fermented liquid.
5. the capillary mensuration of fermented liquid
The bacterial strain that oil extraction is good was cultivated in aforementioned fermention medium 4～5 days, surface tension to its fermented liquid is measured, measure its surface tension value with FD-NST-I Measurement of liquid surface tension coefficient instrument, the bacterial strain that the fermented liquid surface tension value is lower than 40mN/m is preserved, and does the bacterium Physiology and biochemistry and identifies.
Observe and the evaluation of bacterium Physiology and biochemistry through colonial morphology, identify from nine indexs respectively: colonial morphology is observed, the gramstaining test, the oxidation/fermentation type, methyl red (MR) test, gelatin liquification test, the starch hydrolysis experiment, indoles (indole), citrate utilization test (macromethod), the test catalase test, final definite bacterial strain that is filtered out belongs to Pseudomonas aeruginosa (Pseudomonas aeruginosa), this bacterial strain on March 11st, 2010 in China Committee for Culture Collection of Microorganisms's preservation, deposit number is CGMCC No.3661, also be psychrotroph simultaneously, its fermented liquid (0～10 ℃) under low temperature environment still has lower surface tension (all less than 35mN/m).
The result shows, by measuring its fermented liquid surface tension value, and be optimized back medium component and culture condition, medium component is: sucrose 30-31g/L, yeast extract paste 1-1.1g/L, NH
4NO
31-1.1g/L, Na
2HPO
41-1.1g/L, whiteruss 5-6ml, inorganic salt solution volume by volume concentration (v/v) is 3-3.2%, and inoculum size is 5～7%, and pH is controlled at 6.8～7.2,35～37 ℃ of temperature, incubation time is 1-4d.
Separation and Extraction
From the fermentation culture of above-mentioned Pseudomonas aeruginosa, just can separation and Extraction make sophorolipid through some appropriate means, these methods are often to be used in the method for extracting metabolite, for example can utilize sophorolipid and impurity to extract in the difference of aspects such as solubleness, ionic bond power, absorption avidity and molecular weight.For example, centrifugal with 35～37 ℃ of fermented liquids of cultivating 24h, collect flocks, obtain crude product, suspend again with methylene dichloride again, filter, extracting, centrifugal, collecting precipitation is the tensio-active agent behind the purifying.
The bio-surfactant that the psychrotroph bacterial strain is produced carries out infrared spectra evaluation (as shown in Figure 1), and qualification result is that the meta-bolites of psychrotroph bacterial strain is a sophorolipid.
This bio-surfactant can directly be used aforementioned fermented liquid, perhaps fermented liquid is used after separation and purification.
The invention will be further described below in conjunction with embodiment, it should be understood that these embodiment only are used for the purpose of illustration, never limit protection scope of the present invention.
Stalk is rotted to approaching with soil or compost dipping, with alcohol to after carrying out surface sterilization near the stalk that rots, be cut into the long small segment of 1～3cm, the straw small segment that will partly become thoroughly decomposed changes over to sterilization metal tweezers and is equipped with in the 25mL 95% alcoholic acid flat board, taking-up changes new sterilization plate inboard over to after soaking 90s, treat that surperficial alcohol dries that (5～10min) backs change PDA solid medium (potato glucose agar medium over to the metal tweezers of sterilizing, finished product 32-34g, agar powder 8-10g) in the plate, seal plate with plastics film, plate is inverted cultivation 5～10d after putting cultivation 8d, at growth fungal colony edge picking fritter bacterium colony, change in the PDA solid medium plate and cultivate, if find that growth has assorted bacterium, picking colony edge to change in the new plate that the PDA solid medium is housed and continues purifying, obtain cellulose-decomposing bacterium.
Nonionic surface active agent PEG6000 and cellulose-decomposing bacterium are made into complex micro organism fungicide in 1: 1 ratio, complex micro organism fungicide is imposed on the bright stalk, the consumption of complex microorganism preparations is 4% of a fresh straw weight.The bright stalk that will be mixed with complex micro organism fungicide when gather in the crops autumn imposes in the ground, is contrast not execute microbial inoculum and stalk.Crop on the 1 year spring kind carries out farm work routinely.
Through experimental test, complex microorganism preparations of the present invention imposes on the bright stalk, do not compare with the stalk contrast with adding complex microorganism preparations, soil moisture content exceeds 15%, and soil bacteria quantity exceeds 2 times, the quantity of soil phosphorus-solubilizing bacteria improves 40%, the soil nitrification number of bacteria improves 3 times, and crop root length improves 25%, and stem of plant minister degree improves 30%, crop weight improves 32%, and seedling stage, ground biomass improved 45%.
Embodiment 2
Method according to embodiment 1 obtains cellulose-decomposing bacterium.
Pseudomonas aeruginosa is produced the glycolipid and the cellulose-decomposing bacterium that contain rhamnosyl be made into complex micro organism fungicide in 2: 1 ratios, complex micro organism fungicide is imposed on the bright stalk, the consumption of complex microorganism preparations is 4.5% of a fresh straw weight.The bright stalk that will be mixed with complex micro organism fungicide when gather in the crops autumn imposes in the ground, is contrast not execute microbial inoculum and stalk.Crop on the 1 year spring kind carries out farm work routinely.
Through experimental test, complex microorganism preparations of the present invention imposes on the bright stalk, do not compare with the stalk contrast with adding complex microorganism preparations, soil moisture content exceeds 18%, and soil bacteria quantity exceeds 2.5 times, the quantity of soil phosphorus-solubilizing bacteria improves 42%, the soil nitrification number of bacteria improves 3.5 times, and crop root length improves 27%, and stem of plant minister degree improves 32%, crop weight improves 34%, and seedling stage, ground biomass improved 50%.
Embodiment 3
Method according to embodiment 1 obtains cellulose-decomposing bacterium.
To carry out fermentation culture according to the Pseudomonas aeruginosa (deposit number is CGMCC No.3661) that preceding method filters out, medium component is: sucrose 30-31g/L, yeast extract paste 1-1.1g/L, NH
4NO
31-1.1g/L, Na
2HPO
41-1.1g/L, whiteruss 5-6ml, inorganic salt solution volume by volume concentration (v/v) is 3-3.2%, and inoculum size is 5-7%, and pH is controlled at 6.8-7.2,35～37 ℃ of temperature, incubation time is 1-4d, obtains described bio-surfactant fermented liquid.
Above-mentioned bio-surfactant fermented liquid and cellulose-decomposing bacterium are made into complex micro organism fungicide by 1: 2 volume ratio, complex micro organism fungicide is imposed on the bright stalk, the consumption of complex microorganism preparations is 5% of a fresh straw weight.The bright stalk that will be mixed with complex micro organism fungicide when gather in the crops autumn imposes in the ground, is contrast not execute microbial inoculum and stalk.Crop on the 1 year spring kind carries out farm work routinely.
Through experimental test, complex microorganism preparations of the present invention imposes on the bright stalk, do not compare with the stalk contrast with adding complex microorganism preparations, soil moisture content exceeds 20%, and soil bacteria quantity exceeds 3 times, the quantity of soil phosphorus-solubilizing bacteria improves 44%, the soil nitrification number of bacteria improves 4 times, and crop root length improves 30%, and stem of plant minister degree improves 34%, crop weight improves 37%, and seedling stage, ground biomass improved 55%.
Claims (10)
1. complex microorganism preparations, be formulated by 1: 2～2: 1 volume ratio by cellulose-decomposing bacterium and tensio-active agent, described cellulose-decomposing bacterium belongs to Trichoderma filamentous fungus (deposit number is CGMCC No.3662), prepare by the following method: at first stalk is rotted to approaching with soil or compost dipping, with alcohol to after carrying out surface sterilization near the stalk that rots, be cut into the long small segment of 1～3cm, the straw small segment that will partly become thoroughly decomposed changes over to sterilization metal tweezers and is equipped with in the 25mL 95% alcoholic acid flat board, taking-up changes new sterilization plate inboard over to after soaking 90s, treat that surperficial alcohol dries that (5～10min) backs change in the PDA solid medium plate with the metal tweezers of sterilizing, seal plate with plastics film, plate is inverted cultivation 5～10d after putting cultivation 8d, at growth fungal colony edge picking fritter bacterium colony, change in the PDA solid medium plate and cultivate, if find growth assorted bacterium is arranged, the picking colony edge changes in the new plate that the PDA solid medium is housed and continues purifying, obtains cellulose-decomposing bacterium.
2. complex microorganism preparations according to claim 1 is characterized in that described PDA solid medium is: potato glucose agar medium, finished product 32-34g, agar powder 8-10g.
3. complex microorganism preparations according to claim 1 is characterized in that described tensio-active agent is synthetic tensio-active agent or bio-surfactant.
4. complex microorganism preparations according to claim 3 is characterized in that the synthetic tensio-active agent comprises cetyl trimethylammonium bromide, sodium laurylsulfonate, PEG6000, Sodium dodecylbenzene sulfonate, tween etc.
5. complex microorganism preparations according to claim 3, it is characterized in that described bio-surfactant is meant the self-faced active substance that is produced by microorganism, plant or animal, as glycolipid, saccharose lipid, lipopeptid or neutral quasi-grease derivative etc., for example pseudomonas aeruginosa produces the glycolipid that contains rhamnosyl.
6. complex microorganism preparations according to claim 5, wherein said bio-surfactant is the compost of soil sample below the various stalks heap of long-term accumulation and cattle farm, under the low temperature culture condition (5～10 ℃), through the bacterium enrichment culture, blood agar line primary dcreening operation, the blue gel flat band method is sieved again and the Pseudomonas aeruginosa (Pseudomonas aeruginosa) that obtains produces, belong to sophorolipid, described pseudomonas aeruginosa strains on March 11st, 2010 in China Committee for Culture Collection of Microorganisms's preservation, deposit number is CGMCC No.3661, under cold condition, its fermented liquid oil extraction diameter is all greater than 3cm, and the fermented liquid surface tension is lower than 40mN/m.
7. complex microorganism preparations according to claim 6, it is characterized in that described bio-surfactant is the fermented liquid of described Pseudomonas aeruginosa, its fermentation culture is 35～37 ℃ of temperature, carry out under the condition of pH value 6.8～7.2, inoculum size is 5～7%, substratum is: sucrose 30-31g/L, yeast extract paste 1-1.1g/L, NH
4NO
31-1.1g/L, Na
2HPO
41-1.1g/L, whiteruss 5-6ml, inorganic salt solution volume by volume concentration (v/v) is 3-3.2%.
8. a method for preparing the described complex microorganism preparations of claim 1-7 is to form by the suitable proportion mixed preparing by described cellulose-decomposing bacterium and tensio-active agent.Described ratio is the volume ratio preparation by 1: 2～2: 1.
9. the application of a complex microorganism preparations as claimed in claim 1 is characterized in that described complex micro organism fungicide is executed equably on the fresh straw surface.
10. application according to claim 9, the consumption that it is characterized in that described complex microorganism preparations is 4～5% of a fresh straw weight.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580996A (en) * | 2012-01-16 | 2012-07-18 | 扬州工业职业技术学院 | Method for preparing soil composite biological repairing agent by using maize straws and application of repairing agent in soil property improvement |
| CN103230917A (en) * | 2013-03-14 | 2013-08-07 | 东北林业大学 | Forest floor combustible biodegradation agent and use thereof |
| CN104876701A (en) * | 2015-05-14 | 2015-09-02 | 广西大学 | Method for treating agricultural waste generated by mandarin orange cultivation through microbiological method |
| CN106244479A (en) * | 2016-03-02 | 2016-12-21 | 西安华诺环保股份有限公司 | A kind of composite bacteria agent capable for deep-seated oil contaminated soil biological restoration technique |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1657613A (en) * | 2005-01-31 | 2005-08-24 | 湖南大学 | Method for raising green trichoderma cellulase active by biological surfae active agent |
| CN101538604A (en) * | 2009-04-09 | 2009-09-23 | 上海交通大学 | Online production method of rhamnolipid biosurfactant in cellulose hydrolyzation |
-
2010
- 2010-04-13 CN CN201010146397.7A patent/CN101898907B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1657613A (en) * | 2005-01-31 | 2005-08-24 | 湖南大学 | Method for raising green trichoderma cellulase active by biological surfae active agent |
| CN101538604A (en) * | 2009-04-09 | 2009-09-23 | 上海交通大学 | Online production method of rhamnolipid biosurfactant in cellulose hydrolyzation |
Non-Patent Citations (1)
| Title |
|---|
| 《农业环境科学学报》 20091231 刘保平等 一种降解秸秆的丝状真菌的分离和定性方法 559-563 1-10 第28卷, 第3期 2 * |
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| CN102580996A (en) * | 2012-01-16 | 2012-07-18 | 扬州工业职业技术学院 | Method for preparing soil composite biological repairing agent by using maize straws and application of repairing agent in soil property improvement |
| CN102580996B (en) * | 2012-01-16 | 2013-09-11 | 扬州工业职业技术学院 | Method for preparing soil composite biological repairing agent by using maize straws and application of repairing agent in soil property improvement |
| CN103230917A (en) * | 2013-03-14 | 2013-08-07 | 东北林业大学 | Forest floor combustible biodegradation agent and use thereof |
| CN104876701A (en) * | 2015-05-14 | 2015-09-02 | 广西大学 | Method for treating agricultural waste generated by mandarin orange cultivation through microbiological method |
| CN106244479A (en) * | 2016-03-02 | 2016-12-21 | 西安华诺环保股份有限公司 | A kind of composite bacteria agent capable for deep-seated oil contaminated soil biological restoration technique |
| CN106588417A (en) * | 2016-12-20 | 2017-04-26 | 罗永志 | Special organic fertilizer for hawthorn trees, and preparation method thereof |
| CN107502578A (en) * | 2017-09-28 | 2017-12-22 | 林伟 | A kind of decomposed preparation and its purposes in rice straw also Tanaka |
| CN108271776A (en) * | 2017-12-25 | 2018-07-13 | 武汉科诺生物科技股份有限公司 | Bacillus lipopeptid class metabolite is used as the purposes of microbial bacteria agent aid |
| CN108264377A (en) * | 2018-03-12 | 2018-07-10 | 江苏新亿源环保科技有限公司 | A kind of special preparation method for promoting rotten agent of Composting |
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