CN101785440A - Method for improving growth trait of Fugu bimaculatus - Google Patents
Method for improving growth trait of Fugu bimaculatus Download PDFInfo
- Publication number
- CN101785440A CN101785440A CN201010122724A CN201010122724A CN101785440A CN 101785440 A CN101785440 A CN 101785440A CN 201010122724 A CN201010122724 A CN 201010122724A CN 201010122724 A CN201010122724 A CN 201010122724A CN 101785440 A CN101785440 A CN 101785440A
- Authority
- CN
- China
- Prior art keywords
- water
- fish
- fugu
- pond
- density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for improving the growth trait of Fugu bimaculatus, comprising the following steps: (1) selecting female Fugu bimaculatus and male Fugu rubripes at the age of 3 to 5 to carry out parent fish mature-promoting culture annually from Match to April, wherein the quantity ratio is 2:1; (2) using luteotropin releasing hormone d-ala analog (LRH-A) to induce spawn; (3) carrying out artificial insemination on Fugu bimaculatus by semina of the Fugu rubripes; (4) culturing offspring seed; and (5) putting fish fry into pond for culturing when the total length of the fish fry is above 3cm. Adopting the method of the invention, the experimental culturing result shows that in northern areas of China, the average weight of the Fugu bimaculatus cultured for 16 months can reach more than 0.35kg, wherein the weight of a maximum individual is 0.45kg, and the culture cycle is shortened by more than one time, thereby greatly reducing culture cost and increasing the culture benefit of Fugu bimaculatus commercial fish.
Description
Technical field
The present invention relates to a kind of method that improves growth trait of Fugu bimaculatus, belong to aquatic biological genetic breeding technical field.
Background technology
Two spot Fugus (Takifugu bimaculatus) are under the jurisdiction of Tetraodontiformes, Molidae, and east Puffer belongs to.Mainly be distributed in China South Sea, the East Sea, reach southern Yellow Sea, be the temperate zone near sea fishes, generally becoming fish length is 150～400mm.Two spot Fugu delicious meat, nutritious are the good merchantable brands on China's southern area dining table.In recent years, province coastal areas such as Fujian, Jiangsu, Zhejiang, Hebei have all begun two spot Fugus and have propagated artificially, but because the culture-cycle is long, southern area need be cultured 2～3 years, northern area need be cultured and just can be reached marketable fish size (body weight is greater than 300g) in 3～4 years, aquaculture cost height, benefit are low, have limited the development of aquaculture.Adopt the triploid breeding, shorten the culture-cycle, but effect is not remarkable with the yellow Fugu hybridization of chrysanthemum, the method that improves the water temperature of surviving the winter.Therefore, seek effective ways improvement growth trait of Fugu bimaculatus, shorten the culture-cycle, significant to improving two spot Fugu marketable fish culture benefits.
Summary of the invention
The purpose of this invention is to provide a kind of method that improves growth trait of Fugu bimaculatus, improve its growth rate, reduced two spot Fugu marketable fish aquaculture costs, improved culture benefit.
Technical conceive of the present invention is: select in the Fugu genus individual big, the fast Fugu rubripes milter that grows hybridizes with two spot Fugu rauns, and the growth traits of the two spot Fugus of improvement is selected suitable cultivating condition, improve its growth rate thereby reach, shorten the purpose of culture-cycle.
Particularly, method of the present invention may further comprise the steps:
(1) select the breeding parent population:
The annual 3-4 month is to select the two spot Fugu rauns in 3-5 age and the Fugu rubripes milter in 3-5 age at 2: 1 with the quantity ratio;
(2) the parent population accelerating is cultivated and is hastened parturition:
Divide the pond to cultivate two spot Fugus and Fugu rubripes parent population, water temperature is controlled at 18 ± 1 ℃, volume according to water calculates, change water 1/3～1/2 every day, throw something and feed clam worm once by 8% of parent population body weight, after cultivating through 30～40 days acceleratings, inject the dosage of 50 μ g by fish body weight per kilogram and give two spot Fugu injection luteotropin releasing hormone d-ala analogs (LRH-A); Inject the dosage of 30 μ g by fish body weight per kilogram and inject luteotropin releasing hormone d-ala analog (LRH-A) to Fugu rubripes;
(3) artificial insemination and hatching:
The oxytocic drug injection is after 72 hours, select the two spot Fugus of ovum fully-developed to carry out ovum artificial collection, carry out the dry method insemination with the Fugu rubripes seminal fluid, fertilized egg is put into the hatching cylinder after washing ovum through 3-5 time, the hatching water temperature is controlled at 19～20 ℃, and every day is changed water twice in salinity 28 ‰～32 ‰, volume according to water calculates, and changes water 100% at every turn;
(4) seed rearing:
Newly hatched larvae is cultivated density 10,000 tails/m
3～2 ten thousand tails/m
3Newly hatched larvae 2 ages in days begin to add the wheel animalcule through the chlorella reinforced cultivating in cultivating water body, in time replenish wheel animalcule according to the prelarva situation of ingesting, and make wheel animalcule density remain on 15/ml, divide the pond to reduce after 10 days and cultivate density to 0.5 ten thousand tails/m
3～1 ten thousand tails/m
3, begin the artemia nauplii of throwing something and feeding, throw something and feed every day 3～4 times, keep 1～3/ml of artemia nauplii quantity in the water body, during prelarva total length 8～10mm, divide the pond to reduce and cultivate density to 0.2 ten thousand tails/m
3～0.5 ten thousand tails/m
3, changing and throw little halogen worm, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable, when long this of body, divides the pond to reduce and cultivate density to 0.1 ten thousand tails/m when above to 10mm
3～0.2 ten thousand tails/m
3, changing and throw halogen worm adult, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable.Use the sand filtration seawater during seed rearing, water temperature is controlled at 22～25 ℃, and every day is changed water twice in salinity 25 ‰～35 ‰, according to the volume calculating of water, each 1/3～1/2.
(5) culture:
When the fry specification reaches total length 3cm when above, put into the pond and culture, breeding density 1000 tail/mus, 12 ℃～35 ℃ of water temperatures, salinity 15 ‰～35 ‰, body length throw something and feed during less than 5cm big halogen worm, fish gruel, feeding volume is had enough no longer to ingest with 80% juvenile fish and is advisable; The small fish of after fry grows into 5cm, throwing something and feeding, feeding volume is 5%～8% of a fish body weight, changes water once in per 5～7 days, calculates according to the volume of water, changes water 30%～50% at every turn; After fry grows into 0.01kg, carry out the branch pond, reduce cultivation density to 100 tail/mus; Through 13-16 month breed, the fish body weight just can be gone on the market more than reaching 0.3kg.
In described (5) breeding process, in the northern area of China, when the pond water temperature drops to 12 ℃ or when being lower than 12 ℃, fish need be moved into indoor wintering, the phase breeding density of surviving the winter is 30 tails/m
2, the water temperature of surviving the winter is controlled at 12～18 ℃, salinity 15 ‰～35 ‰, and bait is small fish, feeding volume calculates by 3%～5% of fish body weight, changes water once, and changes water 70% at every turn in per 3～5 days; When the pond water temperature is higher than 12 ℃, culturing in the fish immigration pond of surviving the winter.
The beneficial effect that the present invention obtains is:
Adopt the present invention to try to support the result and show, culture 16 monthly average body weight in the northern area of China and reach 0.35kg, maximum individual 0.45kg, minimum individual 0.25kg; Culture-cycle has shortened more than one times, and particularly the northern area of China only needs indoor wintering once, has reduced aquaculture cost significantly, and aquaculture cost drops to 40 yuan/kg by 80 yuan original/kg, has improved culture benefit.
Embodiment
Following examples are used to illustrate the present invention, but not as a limitation of the invention.
Place: the new opening king in Changli County, Qinhuangdao, the Hebei province plant of regenerating
Embodiment 1:
Present embodiment is taked following steps:
(1) selects the breeding parent population
Selected two spot Fugu raun 20 tails in 3～4 ages on April 15th, 2008, Fugu rubripes milter 10 tails.
(2) the parent population accelerating is cultivated and is hastened parturition
Divide the pond to cultivate two spot Fugus and Fugu rubripes parent population, water temperature is controlled at 18～19 ℃, volume according to water calculates, change water 1/3～1/2 every day, throw something and feed clam worm once by 8% of parent population body weight, after accelerating cultivation in 30 days, the dosage that May 15 was injected 50 μ g by fish body weight per kilogram is given two spot Fugus injection luteotropin releasing hormone d-ala analogs (LRH-A); Inject the dosage of 30 μ g by fish body weight per kilogram and inject luteotropin releasing hormone d-ala analog (LRH-A) to Fugu rubripes;
(3) artificial insemination and hatching
The oxytocic drug injection was carried out ovum artificial collection to the two spot Fugu rauns of 3 tails of maturation after 72 hours, carried out the dry method insemination with the Fugu rubripes seminal fluid, obtain fertilized egg 680g altogether, fertilized egg is put into the hatching cylinder after washing ovum through 3 times, and the hatching water is the sand filtration seawater, and water temperature is 19～20 ℃, salinity 31 ‰, change water every day 8 times,, change water 100% at every turn according to the volume calculating of water, May 26, hatching finished, and hatched prelarva 120,000 tails.
(4) seed rearing
It is 1.2 ten thousand tails/m that newly hatched larvae is cultivated density
3Newly hatched larvae 2 ages in days begin to add the wheel animalcule through the chlorella reinforced cultivating in cultivating water body, in time replenish wheel animalcule according to the prelarva situation of ingesting, and make wheel animalcule density remain on 15/ml, divide the pond to reduce after 10 days and cultivate density to 0.5 ten thousand tails/m
3, begin the artemia nauplii of throwing something and feeding, throw something and feed every day 4 times, keep 1～2/ml of artemia nauplii quantity in the water body, during prelarva total length 8～10mm, divide the pond to reduce and cultivate density to 0.3 ten thousand tails/m
3, changing and throw little halogen worm, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable, and when body reaches 10mm when above, divides the pond to reduce and cultivates density to 0.2 ten thousand tails/m
3, changing and throw halogen worm adult, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable.Use the sand filtration seawater during seed rearing, water temperature is controlled at 22 ℃, and salinity 30 ‰ is changed water twice every day, according to the volume calculating of water, each 1/3～1/2.Through 45 days seed rearing, July 1, the fry total length on average reached 3.5cm, cultivated fry 80,000 tails altogether.
(5) culture
July 1 was put into 80 mu of ponds with 80,000 tail fries, in earlier stage (body length is less than 5cm) throw something and feed big halogen worm, fish gruel, feeding volume is 8% of a fish body weight, the later stage small fish of throwing something and feeding, feeding volume is 7%～8% of a fish body weight, 18 ℃～35 ℃ of water temperatures during the breed, salinity 28 ‰ was changed water once in per 5～7 days, volume according to water calculates, change water 30%～50% at every turn, through culturing more than four months, October 10 daily weight average out to 0.08kg.October 15 moved into 2500m with the 6.5 ten thousand tail fries that survive in the pond
3In the indoor wintering pond, breeding density is 30 tails/m
3At overwintering period, water temperature is controlled at 15～17 ℃, salinity 25 ‰, and bait is small fish, feeding volume is 3% of a fish body weight, changes water once in per 3～5 days, according to the volume calculating of water, changes water 50%～70% at every turn.Survival 5.8 ten thousand tails of will surviving the winter on April 25th, 2009 are put into 600 mu of ponds and are formed by the fish that survives the winter.Between mature stage, 15 ℃-35 ℃ of water temperatures, salinity 30 ‰, bait feeding sand lance and anchovy, feeding volume are 5% of fish body weight, when gathering in the crops to October 16, average weight reaches 0.35kg, wherein maximum individual 0.45kg, minimum individual 0.25kg gathers in the crops marketable fish 5.2 ten thousand tails altogether, amount to 18200kg, 70 yuan/kg of price, 127.4 ten thousand yuan of the output values, 54.6 ten thousand yuan of profits.
Buy and execute example 2:
(1) selects the breeding parent population
Be 2: 1 to select two spot Fugu rauns and the Fugu rubripes milter in 3 ages with the quantity ratio annual March, and quantity decide according to the amount of growing seedlings, and generally every pair of spot Fugu rauns can produce about seed 30,000 tails.
(2) the parent population accelerating is cultivated and is hastened parturition
Divide the pond to cultivate two spot Fugus and Fugu rubripes parent population, water temperature is controlled at 18 ℃, volume according to water calculates, change water 1/3 every day, throw something and feed clam worm once, and feeding volume calculates by 8% of parent population body weight, after cultivating through 30 days acceleratings, select sexual gland fully-developed parent population to carry out artificial induced spawning, inject the dosage of 50 μ g by fish body weight per kilogram and give two spot Fugu injection luteotropin releasing hormone d-ala analogs (LRH-A); Inject the dosage of 30 μ g by fish body weight per kilogram and inject luteotropin releasing hormone d-ala analog (LRH-A) to Fugu rubripes;
(3) artificial insemination and hatching
The oxytocic drug injection is after 72 hours, carry out two spot Fugu ovum artificial collections, carry out the dry method insemination with the Fugu rubripes seminal fluid, fertilized egg is put into the hatching cylinder after washing ovum through 5 times, and the sand filtration seawater is used in hatching, and water temperature is controlled at 19 ℃, salinity 28 ‰, change water every day 8 times,, change water 100% at every turn according to the volume calculating of water.
(4) seed rearing
Newly hatched larvae is cultivated density 20,000 tails/m
3Newly hatched larvae 2 ages in days begin to add the wheel animalcule through the chlorella reinforced cultivating in cultivating water body, in time replenish wheel animalcule according to the prelarva situation of ingesting, and make wheel animalcule density remain on 15/ml, divide the pond to reduce after 10 days and cultivate density to 1 ten thousand tails/m
3, begin the artemia nauplii of throwing something and feeding, throw something and feed every day 4 times, keep 3/ml of artemia nauplii quantity in the water body, during prelarva total length 8～10mm, divide the pond to reduce and cultivate density to 0.5 ten thousand tails/m
3, changing and throw little halogen worm, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable, and when body reaches 10mm when above, divides the pond to reduce and cultivates density to 0.2 ten thousand tails/m
3, changing and throw halogen worm adult, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable.Use the sand filtration seawater during seed rearing, water temperature is controlled at 24 ℃, and salinity 25 ‰ is changed water twice every day, according to the volume calculating of water, each 1/2.
(5) culture
When the fry total length reaches 3cm when above, put into the pond and culture, breeding density 1000 tail/mus, water temperature 28-35 ℃, salinity 35 ‰.In earlier stage (body length is less than 5cm) throw something and feed big halogen worm, fish gruel, feeding volume is had enough no longer to ingest with 80% juvenile fish and is advisable.The small fish of throwing something and feeding after fry grows into 5cm, feeding volume is 8% of a fish body weight, changes water once in per 5 days, calculates according to the volume of water, changes water 50% at every turn.After fry grows into 0.01kg, carry out the branch pond, reduce cultivation density to 100 tail/mus.Through 15 months breed, the fish body weight just can be gone on the market more than reaching 0.3kg.
In the northern area of China, when the pond water temperature drops to below 12 ℃, fish need be moved into indoor wintering, the phase breeding density of surviving the winter is 30 tails/m
2, the water temperature of surviving the winter is controlled at 18 ℃, salinity 35 ‰, and bait is small fish, feeding volume calculates by 5% of fish body weight, changes water once in per 5 days, according to the volume calculating of water, changes water 70% at every turn.When the pond water temperature was higher than 12 ℃, fish moved in the piscina surviving the winter.
Embodiment 3:
(1) selects the breeding parent population
Be 2: 1 to select two spot Fugu rauns and the Fugu rubripes milter in 5 ages with the quantity ratio annual April, and quantity decide according to the amount of growing seedlings, and generally every pair of spot Fugu rauns can produce about seed 30,000 tails.
(2) the parent population accelerating is cultivated and is hastened parturition
Divide the pond to cultivate two spot Fugus and Fugu rubripes parent population, water temperature is controlled at 19 ℃, change water 1/2 every day, throw something and feed clam worm once, feeding volume calculates by 8% of parent population body weight, after accelerating cultivation in 40 days, select sexual gland fully-developed parent population to carry out artificial induced spawning, inject the dosage of 50 μ g by fish body weight per kilogram and give two spot Fugus injection luteotropin releasing hormone d-ala analogs (LRH-A); Inject the dosage of 30 μ g by fish body weight per kilogram and inject luteotropin releasing hormone d-ala analog (LRH-A) to Fugu rubripes;
(3) artificial insemination and hatching
The oxytocic drug injection is after 72 hours, carry out two spot Fugu ovum artificial collections, carry out the dry method insemination with the Fugu rubripes seminal fluid, fertilized egg is put into the hatching cylinder after washing ovum through 5 times, and the sand filtration seawater is used in hatching, and water temperature is controlled at 20 ℃, salinity 32 ‰, change water every day 8 times,, change water 100% at every turn according to the volume calculating of water.
(4) seed rearing
Newly hatched larvae is cultivated density 10,000 tails/m
3Newly hatched larvae 2 ages in days begin to add the wheel animalcule through the chlorella reinforced cultivating in cultivating water body, in time replenish wheel animalcule according to the prelarva situation of ingesting, and make wheel animalcule density remain on 15/ml, divide the pond to reduce after 10 days and cultivate density to 0.7 ten thousand tails/m
3, begin the artemia nauplii of throwing something and feeding, throw something and feed every day 4 times, keep 2/ml of artemia nauplii quantity in the water body, during prelarva total length 8～10mm, divide the pond to reduce and cultivate density to 0.4 ten thousand tails/m
3, changing and throw little halogen worm, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable, and when body reaches 10mm when above, divides the pond to reduce and cultivates density to 0.2 ten thousand tails/m
3, changing and throw halogen worm adult, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable.Use the sand filtration seawater during seed rearing, water temperature is controlled at 25 ℃, and salinity 35 ‰ is changed water twice every day, according to the volume calculating of water, each 1/2.
(5) culture
When the fry total length reaches 3cm when above, put into the pond and culture, breeding density 1000 tail/mus, 12 ℃ of water temperatures, salinity 15 ‰.In earlier stage (body length is less than 5cm) throw something and feed big halogen worm, fish gruel, feeding volume is had enough no longer to ingest with 80% juvenile fish and is advisable.The small fish of throwing something and feeding after fry grows into 5cm, feeding volume is 5% of a fish body weight, changes water once in per 5 days, calculates according to the volume of water, changes water 30% at every turn.After fry grows into 0.01kg, carry out the branch pond, reduce cultivation density to 100 tail/mus.Generally through 13 months breed, the fish body weight just can be gone on the market more than reaching 0.3kg.
In the northern area of China, when the pond water temperature drops to 12 ℃, fish need be moved into indoor wintering, the phase breeding density of surviving the winter is 30 tails/m
2, the water temperature of surviving the winter is controlled at 12-15 ℃, salinity 15 ‰, and bait is small fish, feeding volume calculates by 3% of fish body weight, changes water once in per 3 days, according to the volume calculating of water, changes water 70% at every turn.When the pond water temperature was higher than 12 ℃, fish moved in the piscina surviving the winter.
Embodiment 4:
(1) selects the breeding parent population
The annual 3-4 month is to select two spot Fugu rauns and the Fugu rubripes milter in 3～5 ages at 2: 1 with the quantity ratio, and quantity decide according to the amount of growing seedlings, and generally every pair of spot Fugu rauns can produce about seed 30,000 tails.
(2) the parent population accelerating is cultivated and is hastened parturition
Divide the pond to cultivate two spot Fugus and Fugu rubripes parent population, water temperature is controlled at 18 ± 1 ℃, volume according to water calculates, change water 1/3～1/2 every day, throw something and feed clam worm once, and feeding volume calculates by 8% of parent population body weight, after cultivating through 30～40 days acceleratings, select sexual gland fully-developed parent population to carry out artificial induced spawning, inject the dosage of 50 μ g by fish body weight per kilogram and give two spot Fugu injection luteotropin releasing hormone d-ala analogs (LRH-A); Inject the dosage of 30 μ g by fish body weight per kilogram and inject luteotropin releasing hormone d-ala analog (LRH-A) to Fugu rubripes;
(3) artificial insemination and hatching
The oxytocic drug injection is after 72 hours, carry out two spot Fugu ovum artificial collections, carry out the dry method insemination with the Fugu rubripes seminal fluid, fertilized egg is put into the hatching cylinder after washing ovum through 3～5 times, the hatching water temperature is controlled at 19～20 ℃, and every day is changed water 8 times in salinity 28 ‰～32 ‰, volume according to water calculates, and changes water 100% at every turn.Use the sand filtration seawater between the incubation period.
(4) seed rearing
Newly hatched larvae is cultivated density 10,000 tails/m
3～2 ten thousand tails/m
3Newly hatched larvae 2 ages in days begin to add the wheel animalcule through the chlorella reinforced cultivating in cultivating water body, in time replenish wheel animalcule according to the prelarva situation of ingesting, and make wheel animalcule density remain on 15/ml, divide the pond to reduce after 10 days and cultivate density to 0.5 ten thousand tails/m
3～1 ten thousand tails/m
3, begin the artemia nauplii of throwing something and feeding, throw something and feed every day 3～4 times, keep 1～3/ml of artemia nauplii quantity in the water body, during prelarva total length 8～10mm, divide the pond to reduce and cultivate density to 0.2 ten thousand tails/m
3～0.5 ten thousand tails/m
3, changing and throw little halogen worm, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable, and when body reaches 10mm when above, divides the pond to reduce and cultivates density to 0.1 ten thousand tails/m
3～0.2 ten thousand tails/m
3, changing and throw halogen worm adult, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable.Use the sand filtration seawater during seed rearing, water temperature is controlled at 22～25 ℃, and every day is changed water twice in salinity 25 ‰～35 ‰, according to the volume calculating of water, each 1/3～1/2.
(5) culture
When the fry total length reaches 3cm when above, put into the pond and culture, breeding density 1000 tail/mus, 12 ℃～35 ℃ of water temperatures, salinity 15 ‰～35 ‰.In earlier stage (body length is less than 5cm) throw something and feed big halogen worm, fish gruel, feeding volume is had enough no longer to ingest with 80% juvenile fish and is advisable.The small fish of throwing something and feeding after fry grows into 5cm, feeding volume is 5%～8% of a fish body weight, changes water once in per 5～7 days, calculates according to the volume of water, changes water 30%～50% at every turn.After fry grows into 0.01kg, carry out the branch pond, reduce cultivation density to 100 tail/mus.General through individual month breed of 13-16, the fish body weight just can be gone on the market more than reaching 0.3kg.
In the northern area of China, when the pond water temperature drops to below 12 ℃ or 12 ℃, fish need be moved into indoor wintering, the phase breeding density of surviving the winter is 30 tails/m
2, the water temperature of surviving the winter is controlled at 12～18 ℃, salinity 15 ‰～35 ‰, and bait is small fish, feeding volume calculates by 3%～5% of fish body weight, changes water once, and changes water 70% at every turn in per 3～5 days.When the pond water temperature was higher than 12 ℃, fish moved in the piscina surviving the winter.
Claims (2)
1. method that improves growth trait of Fugu bimaculatus is characterized in that may further comprise the steps:
(2) select the breeding parent population:
The annual 3-4 month is to select the two spot Fugu rauns in 3-5 age and the Fugu rubripes milter in 3-5 age at 2: 1 with the quantity ratio;
(2) the parent population accelerating is cultivated and is hastened parturition:
Divide the pond to cultivate two spot Fugus and Fugu rubripes parent population, water temperature is controlled at 18 ± 1 ℃, volume according to water calculates, change water 1/3～1/2 every day, throw something and feed clam worm once by 8% of parent population body weight, after accelerating cultivation in 30～40 days, inject the dosage of 50 μ g by fish body weight per kilogram and give two spot Fugus injection luteotropin releasing hormone d-ala analogs (LRH-A); Inject the dosage of 30 μ g by fish body weight per kilogram and inject luteotropin releasing hormone d-ala analog (LRH-A) to Fugu rubripes;
(3) artificial insemination and hatching:
The oxytocic drug injection is after 72 hours, select the two spot Fugus of ovum fully-developed to carry out ovum artificial collection, carry out the dry method insemination with the Fugu rubripes seminal fluid, fertilized egg is put into the hatching cylinder after washing ovum through 3-5 time, the hatching water temperature is controlled at 19～20 ℃, and every day is changed water twice in salinity 28 ‰～32 ‰, volume according to water calculates, and changes water 100% at every turn;
(4) seed rearing:
Newly hatched larvae is cultivated density 10,000 tails/m
3～2 ten thousand tails/m
3Newly hatched larvae 2 ages in days begin to add the wheel animalcule through the chlorella reinforced cultivating in cultivating water body, in time replenish wheel animalcule according to the prelarva situation of ingesting, and make wheel animalcule density remain on 15/ml, divide the pond to reduce after 10 days and cultivate density to 0.5 ten thousand tails/m
3～1 ten thousand tails/m
3, begin the artemia nauplii of throwing something and feeding, throw something and feed every day 3～4 times, keep 1～3/ml of artemia nauplii quantity in the water body, during prelarva total length 8～10mm, divide the pond to reduce and cultivate density to 0.2 ten thousand tails/m
3～0.5 ten thousand tails/m
3, changing and throw little halogen worm, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable, and when body reaches 10mm when above, divides the pond to reduce and cultivates density to 0.1 ten thousand tails/m
3～0.2 ten thousand tails/m
3, changing and throw halogen worm adult, daily ration, feeding quantity is had enough no longer to ingest with 80% juvenile fish and is advisable.Use the sand filtration seawater during seed rearing, water temperature is controlled at 22～25 ℃, and every day is changed water twice in salinity 25 ‰～35 ‰, according to the volume calculating of water, each 1/3～1/2; (5) culture:
When the fry specification reaches total length 3cm when above, put into the pond and culture, breeding density 1000 tail/mus, 12 ℃～35 ℃ of water temperatures, salinity 15 ‰～35 ‰, body length throw something and feed during less than 5cm big halogen worm, fish gruel, feeding volume is had enough no longer to ingest with 80% juvenile fish and is advisable; The small fish of after fry grows into 5cm, throwing something and feeding, feeding volume is 5%～8% of a fish body weight, changes water once in per 5～7 days, calculates according to the volume of water, changes water 30%～50% at every turn; After fry grows into 0.01kg, carry out the branch pond, reduce cultivation density to 100 tail/mus; Through 13-16 month breed, the fish body weight just can be gone on the market more than reaching 0.3kg.
2. a kind of method that improves growth trait of Fugu bimaculatus according to claim 1, it is characterized in that in described (5) breeding process, in the northern area of China, when the pond water temperature drops to 12 ℃ or when being lower than 12 ℃, fish need be moved into indoor wintering, the phase breeding density of surviving the winter is 30 tails/m
2, the water temperature of surviving the winter is controlled at 12～18 ℃, salinity 15 ‰～35 ‰, and bait is small fish, feeding volume calculates by 3%～5% of fish body weight, changes water once in per 3～5 days, according to the volume calculating of water, changes water 70% at every turn; When the pond water temperature is higher than 12 ℃, culturing in the fish immigration pond of surviving the winter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010122724A CN101785440A (en) | 2010-03-12 | 2010-03-12 | Method for improving growth trait of Fugu bimaculatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010122724A CN101785440A (en) | 2010-03-12 | 2010-03-12 | Method for improving growth trait of Fugu bimaculatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101785440A true CN101785440A (en) | 2010-07-28 |
Family
ID=42528944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010122724A Pending CN101785440A (en) | 2010-03-12 | 2010-03-12 | Method for improving growth trait of Fugu bimaculatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101785440A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613123A (en) * | 2012-04-23 | 2012-08-01 | 浙江省海洋水产研究所 | Overwintering farming method of spotted maigre |
| CN105075936A (en) * | 2015-06-19 | 2015-11-25 | 福建省水产研究所 | Method for quickly cultivating Takifugu bimaculatus |
| CN106172118A (en) * | 2016-07-14 | 2016-12-07 | 天津农学院 | A kind of river fish Seedling mating system |
| CN106852283A (en) * | 2016-12-28 | 2017-06-16 | 大连富泰水产养殖有限公司 | A kind of Fugu rubripes breeding method |
| CN107624682A (en) * | 2017-08-22 | 2018-01-26 | 大连富泰水产养殖有限公司 | A kind of Fugu rubripes cultural method |
| CN109303023A (en) * | 2018-11-20 | 2019-02-05 | 南通龙洋水产有限公司 | A kind of fugu obscurus raun maturation method synchronous with Fugu rubripes milter sexual gland |
| CN111919799A (en) * | 2020-07-13 | 2020-11-13 | 浙江省海洋水产养殖研究所 | Method for debonding viscous fertilized eggs of marine fishes |
| CN115886159A (en) * | 2023-03-09 | 2023-04-04 | 海南热带海洋学院 | Sexual gland development strengthening bait and method for hybridizing puffer fish with puffer fish |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032231A (en) * | 2006-03-09 | 2007-09-12 | 上海能正渔业科技开发有限公司 | Techniques for the artificial propagation and larval rearing of fugu obscurus |
| CN101248770A (en) * | 2008-03-28 | 2008-08-27 | 河北省水产研究所 | Method for shortening cultivation period of Fugu flavidus |
-
2010
- 2010-03-12 CN CN201010122724A patent/CN101785440A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032231A (en) * | 2006-03-09 | 2007-09-12 | 上海能正渔业科技开发有限公司 | Techniques for the artificial propagation and larval rearing of fugu obscurus |
| CN101248770A (en) * | 2008-03-28 | 2008-08-27 | 河北省水产研究所 | Method for shortening cultivation period of Fugu flavidus |
Non-Patent Citations (4)
| Title |
|---|
| 王玉堂: "《海水水产新品种养殖技术》", 31 December 2003, 中国农业出版社 * |
| 蔡志全等: "双斑东方鲀人工繁殖及苗种培育技术的研究", 《水利渔业》 * |
| 钟建兴等: "双斑东方鲀人工繁殖及鱼苗技术", 《台湾海峡》 * |
| 黄建辉: "虾塘大面积培育双斑东方鲀鱼种技术", 《水产养殖》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613123A (en) * | 2012-04-23 | 2012-08-01 | 浙江省海洋水产研究所 | Overwintering farming method of spotted maigre |
| CN105075936A (en) * | 2015-06-19 | 2015-11-25 | 福建省水产研究所 | Method for quickly cultivating Takifugu bimaculatus |
| CN106172118A (en) * | 2016-07-14 | 2016-12-07 | 天津农学院 | A kind of river fish Seedling mating system |
| CN106852283A (en) * | 2016-12-28 | 2017-06-16 | 大连富泰水产养殖有限公司 | A kind of Fugu rubripes breeding method |
| CN107624682A (en) * | 2017-08-22 | 2018-01-26 | 大连富泰水产养殖有限公司 | A kind of Fugu rubripes cultural method |
| CN109303023A (en) * | 2018-11-20 | 2019-02-05 | 南通龙洋水产有限公司 | A kind of fugu obscurus raun maturation method synchronous with Fugu rubripes milter sexual gland |
| CN111919799A (en) * | 2020-07-13 | 2020-11-13 | 浙江省海洋水产养殖研究所 | Method for debonding viscous fertilized eggs of marine fishes |
| CN115886159A (en) * | 2023-03-09 | 2023-04-04 | 海南热带海洋学院 | Sexual gland development strengthening bait and method for hybridizing puffer fish with puffer fish |
| CN115886159B (en) * | 2023-03-09 | 2023-10-20 | 海南热带海洋学院 | Gonad development strengthening bait and hybridization method for puffer fish and puffer fish hexaflumuron |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102763615B (en) | Full-manual breeding method for megalonibea fusca | |
| CN101692791B (en) | Totally artificial breeding method for Coilia ectenes | |
| CN101785440A (en) | Method for improving growth trait of Fugu bimaculatus | |
| CN100464633C (en) | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method | |
| CN103355230B (en) | Schizothorax intensified and quick breeding method | |
| CN101248770B (en) | Method for shortening cultivation period of Fugu flavidus | |
| CN102106326B (en) | Method for three-dimensional and artificial seedlings cultivation of perinereis aibuhitensis | |
| CN104521813A (en) | Carp aquaculture method | |
| CN105613374A (en) | Male and female river crab separated pond culture method | |
| CN104161000B (en) | A kind of cross breeding method of hybridizing carp and Pengzhe fish | |
| CN104604759A (en) | Grass carp culturing method | |
| CN102823533A (en) | Low-salt overwintering and breeding method of portunus trituberculatus | |
| CN103493767B (en) | Odontobulis mpotamophila and Odontobutis yaluensis cross breeding method | |
| CN103004658A (en) | Stichopus japonicus-growing method | |
| CN101707986B (en) | Pelteobagrus vachelli and pseudobagrus ussuriensis cross breeding method | |
| CN102334454B (en) | Method for breeding one-year double-season seedlings of Procambarus clarkii cultivated in ponds | |
| CN105724296A (en) | Method for promoting mating and oogenesis of freshwater shrimps | |
| CN102100195A (en) | Method for breeding apostichopus japonicus selenka | |
| CN102860273B (en) | Crossbreeding method of mud crab | |
| CN108323462B (en) | Cultivation method of low-salt-resistant Arca inflata Reeve seedlings | |
| CN110432190A (en) | A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method | |
| CN101401555B (en) | Industrialized fingerling cultivation method for late cod croaker shaped spotted maigre and special clean fish hardening agent thereof | |
| CN105660486A (en) | Breeding method for intercropping mullet parents and fugu obscurus | |
| CN104304136A (en) | Artificial cultivation method for loach larvae | |
| CN104145868B (en) | A kind of method improving pond loach fry survival rate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20100728 |