CN101768616B - 含稳定聚合酶的反应化合物的干组合物 - Google Patents

含稳定聚合酶的反应化合物的干组合物 Download PDF

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Publication number
CN101768616B
CN101768616B CN200910263837.4A CN200910263837A CN101768616B CN 101768616 B CN101768616 B CN 101768616B CN 200910263837 A CN200910263837 A CN 200910263837A CN 101768616 B CN101768616 B CN 101768616B
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liquid mixture
dry composition
polymerase
aptamer
pcr
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CN101768616A (zh
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T·哈斯林格
T·梅茨勒
A·佩塞尼
H·索贝克
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F Hoffmann La Roche AG
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200910263837.4A 2008-12-19 2009-12-17 含稳定聚合酶的反应化合物的干组合物 Active CN101768616B (zh)

Applications Claiming Priority (2)

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EP08022082 2008-12-19
EP08022082.5 2008-12-19

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CN101768616A CN101768616A (zh) 2010-07-07
CN101768616B true CN101768616B (zh) 2014-07-09

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US (3) US8652811B2 (enExample)
EP (1) EP2202302B1 (enExample)
JP (2) JP5731113B2 (enExample)
CN (1) CN101768616B (enExample)
CA (1) CA2688174C (enExample)
ES (1) ES2394754T3 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2798635A1 (en) * 2010-05-06 2011-11-10 Ibis Biosciences, Inc. Integrated sample preparation systems and stabilized enzyme mixtures
EP2598661B1 (en) 2010-07-26 2017-09-27 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012045668A1 (en) 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis in a rt-pcr reaction buffer
CA2810316A1 (en) 2010-10-04 2012-04-12 F. Hoffmann-La Roche Ag Method for cell lysis and pcr within the same reaction vessel
JP2013005796A (ja) * 2011-05-26 2013-01-10 Arkray Inc 乾燥試薬、乾燥試薬キット、試薬器、乾燥試薬の製造方法
EP2574931B1 (en) 2011-09-29 2017-03-22 Qiagen GmbH Dry composition comprising a control dye
US9994891B2 (en) * 2011-10-11 2018-06-12 Qiagen Gmbh Sample processing method and sample processing cartridge
WO2013068107A1 (en) 2011-11-07 2013-05-16 Qiagen Gmbh Lysis method and lysis composition
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
CN103849548A (zh) 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
EP3249054A1 (en) 2012-12-20 2017-11-29 Biomatrica, INC. Formulations and methods for stabilizing pcr reagents
JP5904153B2 (ja) * 2013-03-29 2016-04-13 ソニー株式会社 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ
CA2912307C (en) 2013-06-11 2022-01-11 Biocartis Nv Biomolecule drying process for long-term storage
US10036061B2 (en) * 2013-12-20 2018-07-31 Roche Molecular Systems, Inc. Oligonucleotide inhibitor of DNA polymerases
EP3942931A1 (en) 2014-06-10 2022-01-26 Biomatrica, INC. Stabilization of thrombocytes at ambient temperatures
EP3015553A1 (de) * 2014-10-30 2016-05-04 Biotecon Diagnostics GmbH Stabilisierte Reaktionsmischung
KR20250047404A (ko) 2015-12-08 2025-04-03 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
GB201716963D0 (en) * 2017-10-16 2017-11-29 Biofortuna Ltd Compositions and methods for use in stabilising nucleic acid amplification assays
CN109486824B (zh) * 2018-11-20 2021-11-16 安徽省昂普拓迈生物科技有限责任公司 一种特异性结合Taq酶的核酸适配体及其筛选方法、应用
CN111961709A (zh) * 2020-01-21 2020-11-20 上海酷乐生物科技有限公司 一种用于口腔拭子直接荧光pcr扩增的试剂和试剂盒
CN120202305A (zh) * 2022-11-18 2025-06-24 日油株式会社 核酸扩增用的干燥组合物以及使用其的核酸扩增法
CN115851889B (zh) * 2022-11-30 2025-12-05 北京全式金生物技术股份有限公司 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3300474A (en) 1964-02-12 1967-01-24 Pharmacia Ab Sucrose ether copolymerizates
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
GB8826429D0 (en) 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
KR970002255B1 (ko) 1990-06-11 1997-02-26 넥스스타 파아마슈티컬드, 인크. 핵산 리간드
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5200399A (en) 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5593824A (en) 1994-09-02 1997-01-14 Pharmacia Biotech, Inc. Biological reagent spheres
US5565318A (en) 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
AU726501B2 (en) 1996-06-04 2000-11-09 University Of Utah Research Foundation Monitoring hybridization during PCR
ATE390491T1 (de) 1996-06-04 2008-04-15 Univ Utah Res Found Behälter zum durchführen und beobachten biologischer prozesse
EP1002875A1 (en) * 1998-11-17 2000-05-24 Mira Diagnostica GmbH An oligonucleotide inhibitor of thermostable polymerase
DK1232502T3 (da) 1999-11-17 2006-05-22 Roche Diagnostics Gmbh Magnetiske glaspartikler, fremgangsmåde til deres fremstilling samt anvendelser deraf
ES2294287T5 (es) 2002-01-08 2015-10-27 F. Hoffmann-La Roche Ag Empleo de un material de sílice en una reacción de amplificación
US7407747B2 (en) * 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
CA2521127C (en) * 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20070117094A1 (en) * 2003-09-30 2007-05-24 Yoshihide Hayashizaki Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
WO2007106580A2 (en) * 2006-03-15 2007-09-20 Micronics, Inc. Rapid magnetic flow assays
ATE555216T1 (de) * 2006-05-23 2012-05-15 Molecular Detection Inc Umgebungstemperaturstabile kits für die molekulare diagnostik
AU2007297484B2 (en) 2006-09-18 2013-07-18 Global Life Sciences Solutions Usa Llc Preparation of glassified biological reagents
DE102006056790B3 (de) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Laboreinmalartikel für Analyse und Diagnosik
KR20100049019A (ko) * 2007-06-16 2010-05-11 이니그마 다이아그노스틱스 리미티드 조성물

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients

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US20100159529A1 (en) 2010-06-24
JP5731113B2 (ja) 2015-06-10
JP2015091261A (ja) 2015-05-14
US9163286B2 (en) 2015-10-20
CN101768616A (zh) 2010-07-07
US20140162267A1 (en) 2014-06-12
US20160237473A1 (en) 2016-08-18
US9631233B2 (en) 2017-04-25
US8652811B2 (en) 2014-02-18
EP2202302A1 (en) 2010-06-30
EP2202302B1 (en) 2012-09-12
ES2394754T3 (es) 2013-02-05
JP2010142229A (ja) 2010-07-01
CA2688174C (en) 2018-08-07
CA2688174A1 (en) 2010-06-19

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