CA2688174C - Dry composition of reaction compounds with stabilized polymerase - Google Patents
Dry composition of reaction compounds with stabilized polymerase Download PDFInfo
- Publication number
- CA2688174C CA2688174C CA2688174A CA2688174A CA2688174C CA 2688174 C CA2688174 C CA 2688174C CA 2688174 A CA2688174 A CA 2688174A CA 2688174 A CA2688174 A CA 2688174A CA 2688174 C CA2688174 C CA 2688174C
- Authority
- CA
- Canada
- Prior art keywords
- reaction compounds
- dry composition
- aptamer
- liquid mixture
- polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08022082 | 2008-12-19 | ||
| EP08022082.5 | 2008-12-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2688174A1 CA2688174A1 (en) | 2010-06-19 |
| CA2688174C true CA2688174C (en) | 2018-08-07 |
Family
ID=40510578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2688174A Active CA2688174C (en) | 2008-12-19 | 2009-12-11 | Dry composition of reaction compounds with stabilized polymerase |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US8652811B2 (enExample) |
| EP (1) | EP2202302B1 (enExample) |
| JP (2) | JP5731113B2 (enExample) |
| CN (1) | CN101768616B (enExample) |
| CA (1) | CA2688174C (enExample) |
| ES (1) | ES2394754T3 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2011249913B2 (en) * | 2010-05-06 | 2014-09-11 | Ibis Biosciences, Inc. | Integrated sample preparation systems and stabilized enzyme mixtures |
| US9845489B2 (en) | 2010-07-26 | 2017-12-19 | Biomatrica, Inc. | Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
| WO2012018638A2 (en) | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
| JP5798631B2 (ja) | 2010-10-04 | 2015-10-21 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｒｔ−ｐｃｒ反応緩衝液中での細胞溶解のための方法 |
| EP2625285B1 (en) | 2010-10-04 | 2020-04-29 | Roche Diagniostics GmbH | Method for cell lysis and pcr within the same reaction vessel |
| JP2013005796A (ja) * | 2011-05-26 | 2013-01-10 | Arkray Inc | 乾燥試薬、乾燥試薬キット、試薬器、乾燥試薬の製造方法 |
| EP2574931B1 (en) | 2011-09-29 | 2017-03-22 | Qiagen GmbH | Dry composition comprising a control dye |
| EP3663408B1 (en) * | 2011-10-11 | 2024-05-01 | QIAGEN GmbH | Sample processing method and sample processing cartridge |
| EP2776577B1 (en) | 2011-11-07 | 2017-01-11 | Qiagen GmbH | Lysis method and lysis composition |
| EP2730653A1 (en) | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
| CN103849548A (zh) | 2012-12-03 | 2014-06-11 | 三星电子株式会社 | 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统 |
| EP2934572A4 (en) | 2012-12-20 | 2016-11-23 | Biomatrica Inc | FORMULATIONS AND METHODS FOR STABILIZING PCR REAGENTS |
| JP5904153B2 (ja) * | 2013-03-29 | 2016-04-13 | ソニー株式会社 | 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ |
| EP3008177B1 (en) | 2013-06-11 | 2020-06-03 | Biocartis NV | Biomolecule drying process for long-term storage |
| US10036061B2 (en) | 2013-12-20 | 2018-07-31 | Roche Molecular Systems, Inc. | Oligonucleotide inhibitor of DNA polymerases |
| WO2015191632A1 (en) | 2014-06-10 | 2015-12-17 | Biomatrica, Inc. | Stabilization of thrombocytes at ambient temperatures |
| EP3015553A1 (de) * | 2014-10-30 | 2016-05-04 | Biotecon Diagnostics GmbH | Stabilisierte Reaktionsmischung |
| ES3037826T3 (en) | 2015-12-08 | 2025-10-07 | Biomatrica Inc | Reduction of erythrocyte sedimentation rate |
| GB201716963D0 (en) * | 2017-10-16 | 2017-11-29 | Biofortuna Ltd | Compositions and methods for use in stabilising nucleic acid amplification assays |
| CN109486824B (zh) * | 2018-11-20 | 2021-11-16 | 安徽省昂普拓迈生物科技有限责任公司 | 一种特异性结合Taq酶的核酸适配体及其筛选方法、应用 |
| CN111961709A (zh) * | 2020-01-21 | 2020-11-20 | 上海酷乐生物科技有限公司 | 一种用于口腔拭子直接荧光pcr扩增的试剂和试剂盒 |
| JPWO2024106507A1 (enExample) * | 2022-11-18 | 2024-05-23 | ||
| CN115851889B (zh) * | 2022-11-30 | 2025-12-05 | 北京全式金生物技术股份有限公司 | 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3300474A (en) | 1964-02-12 | 1967-01-24 | Pharmacia Ab | Sucrose ether copolymerizates |
| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| GB8826429D0 (en) | 1988-11-11 | 1988-12-14 | Univ Leeds Ind Service Ltd | Enzyme stabilisation systems |
| GB8903593D0 (en) | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US5475096A (en) | 1990-06-11 | 1995-12-12 | University Research Corporation | Nucleic acid ligands |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5200399A (en) | 1990-09-14 | 1993-04-06 | Boyce Thompson Institute For Plant Research, Inc. | Method of protecting biological materials from destructive reactions in the dry state |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5593824A (en) | 1994-09-02 | 1997-01-14 | Pharmacia Biotech, Inc. | Biological reagent spheres |
| US5565318A (en) | 1994-09-02 | 1996-10-15 | Pharmacia Biotech, Inc. | Room temperature stable reagent semi-spheres |
| EP0912766B2 (en) | 1996-06-04 | 2011-12-14 | University of Utah Research Foundation | Monitoring hybridization during pcr |
| ES2260562T3 (es) | 1996-06-04 | 2006-11-01 | University Of Utah Research Foundation | Sistema y metodo para llevar a cabo y supervisar procesos biologicos. |
| EP1002875A1 (en) * | 1998-11-17 | 2000-05-24 | Mira Diagnostica GmbH | An oligonucleotide inhibitor of thermostable polymerase |
| EP1232502B1 (en) | 1999-11-17 | 2006-01-11 | Roche Diagnostics GmbH | Magnetic glass particles, method for their preparation and uses thereof |
| DE60316660T3 (de) | 2002-01-08 | 2016-01-28 | Roche Diagnostics Gmbh | Verwendung eines silikamaterials bei der amplifikation |
| US7407747B2 (en) * | 2002-10-15 | 2008-08-05 | Applera Corporation | Method for drying dye-terminator sequencing reagents |
| CA2521127C (en) * | 2003-04-01 | 2014-05-27 | Eragen Biosciences, Inc. | Polymerase inhibitor and method of using same |
| EP1502958A1 (en) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | New detection format for hot start real time polymerase chain reaction |
| JPWO2005030958A1 (ja) * | 2003-09-30 | 2007-11-15 | 独立行政法人理化学研究所 | 酵素が固定されている支持体、印刷物、試薬キット、該支持体の製造法、酵素の保存法及び酵素の再生法 |
| US20060099567A1 (en) * | 2004-04-08 | 2006-05-11 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| WO2006131391A1 (en) * | 2005-06-10 | 2006-12-14 | Epigenomics Ag | Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients |
| JP5254949B2 (ja) * | 2006-03-15 | 2013-08-07 | マイクロニクス， インコーポレイテッド | 一体型の核酸アッセイ |
| US20080050737A1 (en) * | 2006-05-23 | 2008-02-28 | Boaz Arieli | Ambient Temperature Stable Kits for Molecular Diagnostics |
| EP2063866B2 (en) | 2006-09-18 | 2017-08-23 | GE Healthcare Bio-Sciences Corp. | Preparation of glassified biological reagents |
| DE102006056790B3 (de) * | 2006-12-01 | 2008-01-17 | IfP Privates Institut für Produktqualität GmbH | Laboreinmalartikel für Analyse und Diagnosik |
| JP2010529850A (ja) * | 2007-06-16 | 2010-09-02 | エニグマ ディアグノスティックス リミテッド | 組成物 |
-
2009
- 2009-12-11 CA CA2688174A patent/CA2688174C/en active Active
- 2009-12-17 JP JP2009286615A patent/JP5731113B2/ja active Active
- 2009-12-17 ES ES09015595T patent/ES2394754T3/es active Active
- 2009-12-17 EP EP09015595A patent/EP2202302B1/en active Active
- 2009-12-17 CN CN200910263837.4A patent/CN101768616B/zh active Active
- 2009-12-18 US US12/641,747 patent/US8652811B2/en active Active
-
2014
- 2014-01-31 US US14/169,993 patent/US9163286B2/en active Active
-
2015
- 2015-01-09 JP JP2015003548A patent/JP2015091261A/ja active Pending
- 2015-09-16 US US14/855,915 patent/US9631233B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20160237473A1 (en) | 2016-08-18 |
| US8652811B2 (en) | 2014-02-18 |
| EP2202302A1 (en) | 2010-06-30 |
| ES2394754T3 (es) | 2013-02-05 |
| US9163286B2 (en) | 2015-10-20 |
| CN101768616B (zh) | 2014-07-09 |
| EP2202302B1 (en) | 2012-09-12 |
| US20100159529A1 (en) | 2010-06-24 |
| JP2015091261A (ja) | 2015-05-14 |
| JP5731113B2 (ja) | 2015-06-10 |
| JP2010142229A (ja) | 2010-07-01 |
| CN101768616A (zh) | 2010-07-07 |
| US20140162267A1 (en) | 2014-06-12 |
| US9631233B2 (en) | 2017-04-25 |
| CA2688174A1 (en) | 2010-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2688174C (en) | Dry composition of reaction compounds with stabilized polymerase | |
| AU2019368215B2 (en) | Engineered enzymes | |
| CN113584033B (zh) | 一种CRISPR/Cpf1基因编辑系统及其构建方法和在赤霉菌中的应用 | |
| CN104152572B (zh) | 同时检测三种链球菌的三重实时荧光pcr方法及试剂盒 | |
| CN113186140B (zh) | 用于预防和/或治疗宿醉和肝病的基因工程细菌 | |
| CN114959919B (zh) | 一种构建酿酒酵母人工小启动子文库的方法及应用 | |
| CN101775410B (zh) | 一种鸡痘病毒载体穿梭质粒及其应用 | |
| CN107384958B (zh) | 基于反向遗传学构建的rsv反基因组质粒及其应用 | |
| KR101578445B1 (ko) | 구제역 a형 중동 유래 아시아 지역형의 방어항원이 발현되는 재조합 구제역 바이러스 및 그의 제조방법 | |
| CN101302531A (zh) | 一种在大肠杆菌-链霉菌-假单胞菌之间穿梭表达的bac载体及其构建方法 | |
| US6537767B1 (en) | Method for screening antimycotically active substances | |
| CN107151676B (zh) | 高灵敏监测POPs的转荧光蛋白基因鱼的制作与应用 | |
| CN110734480B (zh) | 大肠杆菌分子伴侣GroEL/ES在协助合成植物Rubisco中的应用 | |
| CN111378684B (zh) | 一种热诱导的基因编辑系统CRISPR-Cas12b在陆地棉中的应用 | |
| KR102335524B1 (ko) | 뉴캐슬병 바이러스 벡터 기반 pten 유전자 삽입 재조합 뉴캐슬병 바이러스를 이용한 뇌종양 치료용 암용해바이러스 및 이를 이용한 뇌종양 치료용 조성물 | |
| CN114958838B (zh) | 一组高强度的酿酒酵母人工小启动子 | |
| KR102160215B1 (ko) | 자일로스 이용능이 개선된 야로위아 리폴리티카 균주 및 이의 제조방법 | |
| CN106399373B (zh) | 一种Cas9表达载体 | |
| CN104450768B (zh) | 一种靶向酵母线粒体的穿梭载体及其应用 | |
| CN110747216A (zh) | 一种多基因共表达成套载体及其应用 | |
| CN113684240B (zh) | 利用原位倍增blm基因簇及双报告基因制备博莱霉素高产菌株的方法及其应用 | |
| CN110305873A (zh) | 共编辑标记ben-1sgRNA靶位点及其CRISPR/Cas9共编辑系统和应用 | |
| CN111139210A (zh) | 一种重组甲基营养菌和口服疫苗 | |
| CN117147870B (zh) | 一种基于双细胞系统的抗骨硬化蛋白抗体活性检测方法 | |
| AU2020103156A4 (en) | A TA Cloning Vector Based on Enzyme Digestion and Its Construction Method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20141121 |