CN101688204B - 用于制造重组多克隆蛋白质的方法 - Google Patents

用于制造重组多克隆蛋白质的方法 Download PDF

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CN101688204B
CN101688204B CN2008800174383A CN200880017438A CN101688204B CN 101688204 B CN101688204 B CN 101688204B CN 2008800174383 A CN2008800174383 A CN 2008800174383A CN 200880017438 A CN200880017438 A CN 200880017438A CN 101688204 B CN101688204 B CN 101688204B
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polyclone
protein
cell
clone
antibody
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CN101688204A (zh
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拉斯·S·尼尔森
迪特玛·韦尔古尼
安妮·B·托尔斯特拉普
芬恩·威伯格
克里斯琴·穆勒
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Symphogen AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Non-Volatile Memory (AREA)
CN2008800174383A 2007-05-25 2008-05-21 用于制造重组多克隆蛋白质的方法 Expired - Fee Related CN101688204B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
DKPA200700764 2007-05-25
DKPA200701292 2007-09-07
DKPA200701292 2007-09-07
PCT/DK2008/050116 WO2008145133A2 (en) 2007-05-25 2008-05-21 Method for manufacturing a recombinant polyclonal protein

Publications (2)

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CN101688204A CN101688204A (zh) 2010-03-31
CN101688204B true CN101688204B (zh) 2013-07-03

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CN2008800174383A Expired - Fee Related CN101688204B (zh) 2007-05-25 2008-05-21 用于制造重组多克隆蛋白质的方法

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US (1) US7910332B2 (https=)
EP (1) EP2152872B1 (https=)
JP (2) JP5941616B2 (https=)
KR (1) KR101555658B1 (https=)
CN (1) CN101688204B (https=)
AT (1) ATE481483T1 (https=)
AU (1) AU2008255383B2 (https=)
BR (1) BRPI0812091A2 (https=)
CA (1) CA2683800C (https=)
CY (1) CY1110939T1 (https=)
DE (1) DE602008002593D1 (https=)
DK (1) DK2152872T3 (https=)
HR (1) HRP20100640T1 (https=)
IL (1) IL201395A (https=)
MX (1) MX2009012526A (https=)
NZ (1) NZ581316A (https=)
PL (1) PL2152872T3 (https=)
PT (1) PT2152872E (https=)
SI (1) SI2152872T1 (https=)
TW (1) TW200902548A (https=)
WO (1) WO2008145133A2 (https=)
ZA (1) ZA200908021B (https=)

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SI1583830T1 (sl) 2003-01-07 2006-12-31 Symphogen As Postopek za pripravo rekombinantnih poliklonskih proteinov
DK2121920T3 (da) * 2007-03-01 2011-11-21 Symphogen As Fremgangsmåde til kloning af sammenhørende antistoffer
MX2009012526A (es) * 2007-05-25 2009-12-09 Symphogen As Metodo para fabricar una proteina policlonal recombinante.
CA2695309A1 (en) * 2007-09-07 2009-03-12 Symphogen A/S Methods for recombinant manufacturing of anti-rsv antibodies
US20100311610A1 (en) * 2008-02-01 2010-12-09 Chromocell Corporation CELL LINES AND METHODS FOR MAKING AND USING THEM (As Amended)
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
NZ588651A (en) * 2008-04-23 2012-04-27 Symphogen As Methods for manufacturing a polyclonal protein
CA2726289A1 (en) * 2008-05-28 2009-12-23 Bayer Healthcare Llc Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells
CN102137928A (zh) * 2008-08-29 2011-07-27 西福根有限公司 克隆禽源抗体的方法
WO2010089387A1 (en) 2009-02-09 2010-08-12 Morphosys Ag Production of oligoclonal mixtures of immunoglobulins in single cells
MX347981B (es) 2010-11-01 2017-05-22 Symphogen As Composicion de anticuerpos pan-her.
WO2012068317A2 (en) 2010-11-16 2012-05-24 Excelimmune, Inc. Methods for producing recombinant proteins
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
WO2013164689A2 (en) 2012-05-02 2013-11-07 Lantto, Johan Humanized pan-her antibody compositions
US9790269B2 (en) 2013-02-08 2017-10-17 Misfolding Diagnostics, Inc. Transthyretin antibodies and uses thereof
ES2748295T3 (es) 2014-09-16 2020-03-16 Symphogen As Anticuerpos anti-MET y composiciones
US12404552B2 (en) * 2015-07-17 2025-09-02 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
KR20180096793A (ko) * 2016-01-14 2018-08-29 안톤 바우어 생산 세포주를 제조하는 방법
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
UA129904C2 (uk) 2017-04-05 2025-09-10 Ле Лаборатуар Сервьє Комбінація антитіла до tim-3 й антитіла до pd-1 для лікування раку
EP3810649A1 (en) * 2018-06-22 2021-04-28 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CA3118692A1 (en) 2018-11-06 2020-05-14 Alsatech, Inc. Cell-based gene therapy for neurodegenerative diseases
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
CN113728003A (zh) 2019-04-30 2021-11-30 吉加根公司 重组多克隆蛋白及其使用方法
EP4034637A1 (en) * 2019-09-27 2022-08-03 GlaxoSmithKline Intellectual Property Development Limited Method of predicting production stability of clonal cell lines
WO2021253002A1 (en) 2020-06-12 2021-12-16 Gigagen, Inc. Recombinant polyclonal proteins targeting covid-19 and methods of use thereof
EP4713360A1 (en) 2023-05-19 2026-03-25 Les Laboratoires Servier Anti-met antibodies, antibody-drug conjugates, compositions and uses thereof

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WO2004029284A2 (en) * 2002-09-30 2004-04-08 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
US20050170398A1 (en) * 2002-07-18 2005-08-04 Van Berkel Patrick Hendrikus C. Recombinant production of mixtures of antibodies
CN1723283A (zh) * 2003-01-07 2006-01-18 西福根有限公司 用于生产重组多克隆蛋白质的方法

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SI1583830T1 (sl) * 2003-01-07 2006-12-31 Symphogen As Postopek za pripravo rekombinantnih poliklonskih proteinov
US20060275766A1 (en) 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
EA014182B1 (ru) * 2004-07-20 2010-10-29 Симфоген А/С КОМПОЗИЦИЯ ПОЛИКЛОНАЛЬНЫХ АНТИ-RhD АНТИТЕЛ, СПОСОБ ЕЁ ПОЛУЧЕНИЯ И ПРИМЕНЕНИЕ КОМПОЗИЦИИ
US8288109B2 (en) * 2004-07-20 2012-10-16 Symphogen A/S Procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line
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US20050170398A1 (en) * 2002-07-18 2005-08-04 Van Berkel Patrick Hendrikus C. Recombinant production of mixtures of antibodies
WO2004029284A2 (en) * 2002-09-30 2004-04-08 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
CN1723283A (zh) * 2003-01-07 2006-01-18 西福根有限公司 用于生产重组多克隆蛋白质的方法

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Publication number Publication date
NZ581316A (en) 2012-02-24
SI2152872T1 (sl) 2011-01-31
HRP20100640T1 (hr) 2010-12-31
EP2152872B1 (en) 2010-09-15
JP2011504721A (ja) 2011-02-17
PL2152872T3 (pl) 2011-03-31
BRPI0812091A2 (pt) 2014-10-14
CY1110939T1 (el) 2015-06-10
PT2152872E (pt) 2010-11-17
CA2683800A1 (en) 2008-12-04
CA2683800C (en) 2015-03-17
EP2152872A2 (en) 2010-02-17
HK1134519A1 (en) 2010-04-30
WO2008145133A3 (en) 2009-02-05
DE602008002593D1 (de) 2010-10-28
DK2152872T3 (da) 2010-12-06
CN101688204A (zh) 2010-03-31
AU2008255383B2 (en) 2012-11-01
AU2008255383A1 (en) 2008-12-04
KR101555658B1 (ko) 2015-09-24
JP5941616B2 (ja) 2016-06-29
ZA200908021B (en) 2010-11-24
US20090111142A1 (en) 2009-04-30
US7910332B2 (en) 2011-03-22
KR20100017923A (ko) 2010-02-16
JP2015037411A (ja) 2015-02-26
WO2008145133A2 (en) 2008-12-04
ATE481483T1 (de) 2010-10-15
IL201395A0 (en) 2011-08-01
IL201395A (en) 2015-09-24
MX2009012526A (es) 2009-12-09
TW200902548A (en) 2009-01-16

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