TW200902548A - Method for manufacturing a recombinant polyclonal protein - Google Patents

Method for manufacturing a recombinant polyclonal protein Download PDF

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Publication number
TW200902548A
TW200902548A TW097119118A TW97119118A TW200902548A TW 200902548 A TW200902548 A TW 200902548A TW 097119118 A TW097119118 A TW 097119118A TW 97119118 A TW97119118 A TW 97119118A TW 200902548 A TW200902548 A TW 200902548A
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TW
Taiwan
Prior art keywords
cell
cells
protein
expression
cell line
Prior art date
Application number
TW097119118A
Other languages
English (en)
Chinese (zh)
Inventor
Lars Soegaard Nielsen
Dietmar Weilguny
Anne Bondgaard Tolstrup
Finn C Wiberg
Christian Muller
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Publication of TW200902548A publication Critical patent/TW200902548A/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Non-Volatile Memory (AREA)
TW097119118A 2007-05-25 2008-05-23 Method for manufacturing a recombinant polyclonal protein TW200902548A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
DKPA200701292 2007-09-07

Publications (1)

Publication Number Publication Date
TW200902548A true TW200902548A (en) 2009-01-16

Family

ID=39739965

Family Applications (1)

Application Number Title Priority Date Filing Date
TW097119118A TW200902548A (en) 2007-05-25 2008-05-23 Method for manufacturing a recombinant polyclonal protein

Country Status (22)

Country Link
US (1) US7910332B2 (https=)
EP (1) EP2152872B1 (https=)
JP (2) JP5941616B2 (https=)
KR (1) KR101555658B1 (https=)
CN (1) CN101688204B (https=)
AT (1) ATE481483T1 (https=)
AU (1) AU2008255383B2 (https=)
BR (1) BRPI0812091A2 (https=)
CA (1) CA2683800C (https=)
CY (1) CY1110939T1 (https=)
DE (1) DE602008002593D1 (https=)
DK (1) DK2152872T3 (https=)
HR (1) HRP20100640T1 (https=)
IL (1) IL201395A (https=)
MX (1) MX2009012526A (https=)
NZ (1) NZ581316A (https=)
PL (1) PL2152872T3 (https=)
PT (1) PT2152872E (https=)
SI (1) SI2152872T1 (https=)
TW (1) TW200902548A (https=)
WO (1) WO2008145133A2 (https=)
ZA (1) ZA200908021B (https=)

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Publication number Priority date Publication date Assignee Title
SI1583830T1 (sl) 2003-01-07 2006-12-31 Symphogen As Postopek za pripravo rekombinantnih poliklonskih proteinov
DK2121920T3 (da) * 2007-03-01 2011-11-21 Symphogen As Fremgangsmåde til kloning af sammenhørende antistoffer
MX2009012526A (es) * 2007-05-25 2009-12-09 Symphogen As Metodo para fabricar una proteina policlonal recombinante.
CA2695309A1 (en) * 2007-09-07 2009-03-12 Symphogen A/S Methods for recombinant manufacturing of anti-rsv antibodies
US20100311610A1 (en) * 2008-02-01 2010-12-09 Chromocell Corporation CELL LINES AND METHODS FOR MAKING AND USING THEM (As Amended)
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
NZ588651A (en) * 2008-04-23 2012-04-27 Symphogen As Methods for manufacturing a polyclonal protein
CA2726289A1 (en) * 2008-05-28 2009-12-23 Bayer Healthcare Llc Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells
CN102137928A (zh) * 2008-08-29 2011-07-27 西福根有限公司 克隆禽源抗体的方法
WO2010089387A1 (en) 2009-02-09 2010-08-12 Morphosys Ag Production of oligoclonal mixtures of immunoglobulins in single cells
MX347981B (es) 2010-11-01 2017-05-22 Symphogen As Composicion de anticuerpos pan-her.
WO2012068317A2 (en) 2010-11-16 2012-05-24 Excelimmune, Inc. Methods for producing recombinant proteins
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
WO2013164689A2 (en) 2012-05-02 2013-11-07 Lantto, Johan Humanized pan-her antibody compositions
US9790269B2 (en) 2013-02-08 2017-10-17 Misfolding Diagnostics, Inc. Transthyretin antibodies and uses thereof
ES2748295T3 (es) 2014-09-16 2020-03-16 Symphogen As Anticuerpos anti-MET y composiciones
US12404552B2 (en) * 2015-07-17 2025-09-02 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
KR20180096793A (ko) * 2016-01-14 2018-08-29 안톤 바우어 생산 세포주를 제조하는 방법
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
UA129904C2 (uk) 2017-04-05 2025-09-10 Ле Лаборатуар Сервьє Комбінація антитіла до tim-3 й антитіла до pd-1 для лікування раку
EP3810649A1 (en) * 2018-06-22 2021-04-28 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CA3118692A1 (en) 2018-11-06 2020-05-14 Alsatech, Inc. Cell-based gene therapy for neurodegenerative diseases
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
CN113728003A (zh) 2019-04-30 2021-11-30 吉加根公司 重组多克隆蛋白及其使用方法
EP4034637A1 (en) * 2019-09-27 2022-08-03 GlaxoSmithKline Intellectual Property Development Limited Method of predicting production stability of clonal cell lines
WO2021253002A1 (en) 2020-06-12 2021-12-16 Gigagen, Inc. Recombinant polyclonal proteins targeting covid-19 and methods of use thereof
EP4713360A1 (en) 2023-05-19 2026-03-25 Les Laboratoires Servier Anti-met antibodies, antibody-drug conjugates, compositions and uses thereof

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
EP0530216A4 (en) 1990-04-19 1993-06-30 Medimmune, Inc. Administration of monoclonal antibodies against respiratory viruses
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
EP1293564A4 (en) * 2000-06-08 2005-08-17 Mitsubishi Pharma Corp EXPRESSION VECTOR FOR SCREENING CELLS HIGH EXPRESSION OF RECOMBINANT PROTEINS, TRANSFORMING HIGH EXPRESSION OF A RECOMBINANT PROTEIN AND USE THEREOF
US6849259B2 (en) 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
DK2314629T4 (da) * 2002-07-18 2023-02-06 Merus Nv Rekombinant produktion af blandinger af antistoffer
US20040115814A1 (en) * 2002-09-30 2004-06-17 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
SI1583830T1 (sl) * 2003-01-07 2006-12-31 Symphogen As Postopek za pripravo rekombinantnih poliklonskih proteinov
US20060275766A1 (en) 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
EA014182B1 (ru) * 2004-07-20 2010-10-29 Симфоген А/С КОМПОЗИЦИЯ ПОЛИКЛОНАЛЬНЫХ АНТИ-RhD АНТИТЕЛ, СПОСОБ ЕЁ ПОЛУЧЕНИЯ И ПРИМЕНЕНИЕ КОМПОЗИЦИИ
US8288109B2 (en) * 2004-07-20 2012-10-16 Symphogen A/S Procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line
US7850965B2 (en) 2005-12-05 2010-12-14 Symphogen A/S Anti-orthopoxvirus recombinant polyclonal antibody
WO2007101441A1 (en) 2006-03-06 2007-09-13 Symphogen A/S Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections
MX2009012526A (es) * 2007-05-25 2009-12-09 Symphogen As Metodo para fabricar una proteina policlonal recombinante.

Also Published As

Publication number Publication date
NZ581316A (en) 2012-02-24
SI2152872T1 (sl) 2011-01-31
HRP20100640T1 (hr) 2010-12-31
EP2152872B1 (en) 2010-09-15
JP2011504721A (ja) 2011-02-17
PL2152872T3 (pl) 2011-03-31
BRPI0812091A2 (pt) 2014-10-14
CY1110939T1 (el) 2015-06-10
PT2152872E (pt) 2010-11-17
CA2683800A1 (en) 2008-12-04
CA2683800C (en) 2015-03-17
EP2152872A2 (en) 2010-02-17
HK1134519A1 (en) 2010-04-30
WO2008145133A3 (en) 2009-02-05
DE602008002593D1 (de) 2010-10-28
DK2152872T3 (da) 2010-12-06
CN101688204A (zh) 2010-03-31
AU2008255383B2 (en) 2012-11-01
AU2008255383A1 (en) 2008-12-04
KR101555658B1 (ko) 2015-09-24
JP5941616B2 (ja) 2016-06-29
ZA200908021B (en) 2010-11-24
US20090111142A1 (en) 2009-04-30
US7910332B2 (en) 2011-03-22
KR20100017923A (ko) 2010-02-16
JP2015037411A (ja) 2015-02-26
WO2008145133A2 (en) 2008-12-04
ATE481483T1 (de) 2010-10-15
IL201395A0 (en) 2011-08-01
IL201395A (en) 2015-09-24
MX2009012526A (es) 2009-12-09
CN101688204B (zh) 2013-07-03

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