CN101623009A - Botanical pesticide for inhibiting vegetable botrytis cinerea and application thereof - Google Patents
Botanical pesticide for inhibiting vegetable botrytis cinerea and application thereof Download PDFInfo
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- CN101623009A CN101623009A CN200910184051A CN200910184051A CN101623009A CN 101623009 A CN101623009 A CN 101623009A CN 200910184051 A CN200910184051 A CN 200910184051A CN 200910184051 A CN200910184051 A CN 200910184051A CN 101623009 A CN101623009 A CN 101623009A
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- botrytis cinerea
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- vegetable botrytis
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Abstract
The invention discloses a botanical pesticide for inhibiting vegetable botrytis cinerea and an application thereof. The botanical pesticide for inhibiting vegetable botrytis cinerea is a novel low-toxic biological product that takes amorpha fruticosa as raw material, used by spraying on leaves, and is applicable to vegetables. The botanical pesticide for inhibiting vegetable botrytis cinerea takes amorpha fruticosa as raw material; alcohol is used for extraction for 2-4h, the extract liquor is concentrated into solid concentrate, and the solvent is recycled; the solid concentrate is extracted with n-butyl alcohol for 2 to 3 times, the extract liquor is concentrated to obtain solid material extracted by n-butyl alcohol; solid material extracted with n-butyl alcohol is dissolved in n-butyl alcohol that is 0.5-2.0 times of the weight of the solid material, emulsifier that is 0.1-2.0 time of the weight of the solid concentrate is added, after fully stirring, water that is 18-25 times of the weight of the solid concentrate is added in to obtain the emulsion in water of the botanical pesticide for inhibiting vegetable botrytis cinerea. The botanical pesticide for inhibiting vegetable botrytis cinerea can effectively inhibit vegetable botrytis cinerea and can enhance the capability of crops on resistance of pest diseases.
Description
Technical field
The present invention relates to a kind of inhibition vegetable botrytis cinerea (Botrytis cinerea) botanical pesticide (transient name), it is a kind of low toxicity, the new high-efficiency biological product of raw material development with shrubby flase indigo (Amorphya fruticos) plant, mode dispenser with foliage spray, be applied to vegetables, fruit, flowers and other crops, effectively suppress vegetable botrytis cinerea, noresidue is polluted, and has important function for development organic farming and green agriculture.
Background technology
Grey mold Pseudomonas Deuteromycotina Botrytis.The botrytis cinerea conidiophore is elongated, grey black, size is (960～1200) μ m * (16～22) μ m, irregular arborizations, the apical cell of branch expands globulate, on it give birth to many stigmas, on the stigma living spore estranged, likeness in form grape spike.The conidium unit cell is colourless, ellipse or oval, and size is (8～14) μ m * (6～9) μ m, the spore collection is given birth to gray.The sclerotium brown, out-of-shape, size are (2～4) mm * (1～3) mm[1].Botrytis cinerea can infect 200 various plants, belongs to fungal disease, the flower of various plants, really, leaf, stem all can fall ill.This sclerotium is survived the winter the summer on soil or invalid body, and germ is low temperature resistant, 7-20 ℃ of a large amount of the generation spore, temperature of shed 15-23 ℃ of seedling stage.The low light level, relative moisture is more than 90% or seedling is surperficial when moisture film is arranged susceptible disease.Florescence is the easiest to be susceptible, borrows air-flow, and irrigation and farming operation are invaded from wound, old and feeble organ.At present domestic agricultural chemicals to the gray mold control is mainly chemical products, mainly contains: the mould prestige of second, procymidone, iprodione, dimethachlon, phonetic mould amine, jinggangmeisu etc.Therefore, research is suitable, and to prevent and treat the gray mold novel biopesticide significant.
Summary of the invention
The purpose of this invention is to provide a kind of botanical pesticide for inhibiting vegetable botrytis cinerea, this novel pesticide is all pollution-free in production and use, and product is easily degraded, and noresidue is polluted, and value of environmental protection height has good inhibition effect to vegetable botrytis cinerea.
Another object of the present invention provides the application of this botanical pesticide aspect the inhibition vegetable botrytis cinerea.
The objective of the invention is to realize by following measure:
A kind of botanical pesticide for inhibiting vegetable botrytis cinerea is characterized in that it is a raw material with the shrubby flase indigo, adopts its bright leaf or cured leaf, pulverizes, and puts into apparatus,Soxhlet's, with 0.5-2 doubly heavy alcohol extract 2-4 hour, extract is concentrated into solid shape concentrate, reclaims solvent; Extract 2-3 time respectively with the 3-5 n-butanol that doubly the shape concentrate is heavy admittedly, concentrated extract obtains the decorating film that n-butanol extracts again;
Get the decorating film that n-butanol extracts, be dissolved in the n-butanol for its 0.5-2.0 times of weight, be incorporated as the doubly heavy emulsifier of solid shape concentrate 0.1-0.2, fully stir, be incorporated as the doubly heavy water of solid shape concentrate 18-25 again, promptly make the aqueous emulsion of botanical pesticide for inhibiting vegetable botrytis cinerea.
Described raw material is preferably selected the shrubby flase indigo plant of wild or plantation fresh or that dry for use;
The organic solvent of preparation aqueous emulsion is selected n-butanol (technical grade) nonhazardous for use.
Emulsifier is selected span-20, span-40 or span-60 for use, all is conventional surfactant, all nonhazardouss.
Beneficial effect of the present invention:
Botanical pesticide for inhibiting vegetable botrytis cinerea of the present invention is to take from plant effective component extracting to make the method for product again, and production technology is simple, and flow process is short; Solvent can reclaim repeated use, and production cost is low, easily industrialization, economic benefit height.
Raw material of the present invention is shrubby flase indigo (being rich in multiple desinsection anthelmintic ingredient in the plant), contains the various active composition in the extract, and the multiple damage by disease and insect of vegetables is had certain preventive and therapeutic effect.
Solvent of the present invention is a n-butanol, and the product with n-butanol extract is made has good inhibition effect to vegetable botrytis cinerea.
Inhibition vegetable botrytis cinerea plant resource novel pesticide of the present invention, the raw materials used thing that dries for the bright leaf of shrubby flase indigo and tender stem or bright leaf and tender stem.This plant is perennial shrub, well developed root system, and vitality is extremely strong, is fit to various ambient growth, waits idle place all can plant before deserted mountain barren hill, roadside, the room behind the house, does not need management, can tap 2-3 time in 1 year, and raw material sources are easy, cheap.
About the shrubby flase indigo bioactivity research both at home and abroad since the forties, the having of main report: expelling parasite, mosquito repellent; Reporting that medically shrubby flase indigo element and shrubby flase indigo glucoside have calm and anti-spasm effect; The fruit extract of having reported shrubby flase indigo aspect prevention and elimination of disease and pests has control colorado potato beetle, locust effect, to honeybee safety.The base of leaf extract of also having reported shrubby flase indigo has active function to scarab beetle, cabbage caterpillar, to stimulating plant growth obvious effect is arranged.But the base of leaf extract of not seeing shrubby flase indigo is effectively reported botrytis cinerea.
The shrubby flase indigo aboundresources is shrub plant, easily plucks, but and continued growth after tapping, the north 1 year can borrow 2 times, and the 2-3 defective material can be adopted in south, and raw material is easy to get.The present invention utilizes extract sample preparation from the shrubby flase indigo plant, has utilized the function of multiple active principle in the plant corpus, and botrytis cinerea is had good inhibitory action, has avoided the defective of the single component effect of chemosynthesis.This research product is a biologic product, and raw material is renewable, and product is easily degraded after using, and noresidue is polluted, and meets nuisanceless novel pesticide research direction fully.
Embodiment
Embodiment 1
Raw material: fresh wild or plantation shrubby flase indigo plant.Organic solvent: ethanol, n-butanol (technical grade).Emulsifier: span-20.
To put into apparatus,Soxhlet's after the shrubby flase indigo raw material 100kg disorganize, add 50kg ethanol, heating was extracted 2-4 hour, and extract is concentrated into solid shape concentrate, must consolidate shape concentrate 1.5kg; To consolidate shape concentrate 4.5kg extracting n-butyl alcohol, extract respectively 3 times, combining extraction liquid, obtain the decorating film 1kg that n-butanol extracts again behind the concentrated extract, add the 0.6kg n-butanol again, after dissolving under the 80-95 ℃ of heated and stirred, the span-20 that adds 0.1kg, fully stir, add entry again, promptly make 4% botanical pesticide for inhibiting vegetable botrytis cinerea aqueous emulsion to 25kg.
Embodiment 2:
Raw material: fresh wild or plantation shrubby flase indigo plant.Organic solvent: ethanol, n-butanol (technical grade).Emulsifier: span-40.
Amorpha fruticosa leaf and stem 100kg are put into apparatus,Soxhlet's, add the ethanol of 100kg, heating was extracted 4-6 hour, and extract is concentrated into solid shape concentrate; Must consolidate shape concentrate 1.8kg; To consolidate 7kg extracting n-butyl alcohol 3 times of shape concentrate, combining extraction liquid, obtain the decorating film 1.1kg that n-butanol extracts again behind the concentrated extract, add the 1.1kg n-butanol again, after dissolving under the 80-95 ℃ of heated and stirred, add the span-40 of 0.15kg, fully stir, add entry again to 22kg, promptly make 5% botanical pesticide for inhibiting vegetable botrytis cinerea aqueous emulsion.
Embodiment 3:
Raw material: the wild or plantation shrubby flase indigo plant of drying.Organic solvent: ethanol, n-butanol (technical grade).Emulsifier: span-60.
After amorpha fruticosa leaf and stem 100kg dried and pulverizing, put into apparatus,Soxhlet's, add the ethanol of 200kg, heating was extracted 6-8 hour, and extract is concentrated into solid shape concentrate, must consolidate shape concentrate 2.0kg; To consolidate 9kg extracting n-butyl alcohol 3 times of shape concentrate, combining extraction liquid, obtain the decorating film 1.0kg that n-butanol extracts again behind the concentrated extract, add the 2kg n-butanol again, after dissolving under the 80-95 ℃ of heated and stirred, add the span-60 of 0.18kg, fully stir, add entry again to 25kg, promptly make 4% botanical pesticide for inhibiting vegetable botrytis cinerea aqueous emulsion.
The main component of vegetable botrytis cinerea botanical pesticide
Shrubby flase indigo is that the mixture 10g that extracts of raw material is through Chemical Decomposition and structure determination, botanical pesticide for inhibiting vegetable botrytis cinerea mainly contains flavonoids, wherein representation compound is: 6a-12a-dehydrogenation-a-bastard indigo phenol 30-50mg, 6a-12a-dehydrogenation-deguelin 80-110mg, (±)-tephrosin 140-180mg, (-)-6-hydroxyl-6a, 12a-dehydrogenation-bastard indigo phenol 80-110mg, general flavone is 150-250mg.
Shrubby flase indigo extract variable concentrations bacteriostatic activity is measured
The colony diameter experiment
Make a certain amount of PDA medium, after sterilization is cooled to uniform temperature, get the aqueous emulsion (product that embodiment 1 makes) of shrubby flase indigo n-butanol extract respectively, add the concentration series (10 that water is made soup
4, 10
3, 10
2, 50.0,10.0mg/L) 2mL and 18mlPDA medium mixing, injecting diameter is the sterile petri dish of 9cm, obtains handling that dull and stereotyped (it is 10 that concentration is
3, 10
2, 10.0,5.0,1.0mg/L).Drying the back is the botrytis cinerea bacterium piece of 6mm at dull and stereotyped central authorities access diameter, every kind of 5 repetitions, and the gained data are as shown in table 1.Make comparisons with the mould prestige of the reasonable second of effect that use present market, the result adds up in table 2.Solvent and sterile water contrast, and replace the shrubby flase indigo extract and the medium mixing of respective amount with the sterile water of same amount.After bacterium colony to be contrasted is covered with culture dish, measure colony diameter, record data.Calculate bacteriostasis rate according to following formula;
Bacteriostasis rate (%)=(contrast colony diameter-processing colony diameter)/(the contrast colony diameter-6mm) * 100%
The bacteriostatic diameter (botrytis cinerea) of table 1 variable concentrations shrubby flase indigo n-butanol extract
The bacteriostatic diameter of the mould prestige of table 2 variable concentrations second
Relatively drawn by table 1～2, it is the highest that the shrubby flase indigo n-butanol extract is made (concentration is 1%) inhibition botrytis cinerea rate, reaches 63.01%, and working concentration is 1g/L.Than the mould prestige bacteriostasis rate of second improvement value be: 63.01%-53.49%=9.52%.
Advantage of the present invention:
One, little toxicity, noresidue.The vegetable botrytis cinerea botanical pesticide derives from the extract in nonhazardous plant-shrubby flase indigo, and formulated, sintetics non-chemically, after measured, rat acute per os: LD
50＞5000mg/kg belongs to the micro-virus kind agricultural chemicals, and all pollution-free in production and the use, product is easily degraded, and noresidue is polluted, value of environmental protection height.
Two, inhibition vegetable botrytis cinerea effect is remarkable.Suppressing vegetable botrytis cinerea is major function, also is principal character.Test shows mainly and shows: 1% botanical pesticide for inhibiting vegetable botrytis cinerea is 63.01% to the botrytis cinerea inhibiting rate.
Three, regulate growth, raising quality.The foliage spray botanical pesticide for inhibiting vegetable botrytis cinerea.Also can increase chlorophyll and plant dry matter content, improve output and integrated quality 5-10% simultaneously.Has higher commodity value.
Four, other diseases prevention, insect protected function.Product has various pests such as aphid, fusarium wilt, leaf class diseases necessarily prevents and treats function, makes it be difficult for forming the large tracts of land insect pest.
Five, the comparison of like product
Because the present domestic similar biological product that do not have, the product that has only chemosynthesis, no comparativity, but show by table 1～2 comparative experiments results: the shrubby flase indigo n-butanol extract is to the bacteriostasis rate 63.01% of botrytis cinerea, the bacteriostasis rate 53.49% of the mould prestige of second improves 9.52%.
Claims (4)
1, a kind of botanical pesticide for inhibiting vegetable botrytis cinerea is characterized in that it is raw material with the shrubby flase indigo, adopts its bright leaf or cured leaf, pulverize, put into apparatus,Soxhlet's, with 0.5-2 doubly heavy alcohol extract 2-4 hour, extract is concentrated into solid shape concentrate, reclaims solvent; Extract 2-3 time respectively with the 3-5 n-butanol that doubly the shape concentrate is heavy admittedly, concentrated extract obtains the decorating film that n-butanol extracts again;
Get the decorating film that n-butanol extracts, be dissolved in the n-butanol for its 0.5-2.0 times of weight, be incorporated as the doubly heavy emulsifier of solid shape concentrate 0.1-0.2, fully stir, be incorporated as the doubly heavy water of solid shape concentrate 18-25 again, promptly make the aqueous emulsion of botanical pesticide for inhibiting vegetable botrytis cinerea.
2, botanical pesticide for inhibiting vegetable botrytis cinerea according to claim 1 and 2 is characterized in that described emulsifier selects span-20, span-40 or span-60 for use.
3, botanical pesticide for inhibiting vegetable botrytis cinerea according to claim 1 is characterized in that described raw material selects for use the leaf of shrubby flase indigo plant of wild or plantation and the aquatic foods material or the bright material of tender stem to dry thing.
4, claim 1, the application of 2 or 3 described botanical pesticides aspect the inhibition vegetable botrytis cinerea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910184051A CN101623009A (en) | 2009-08-12 | 2009-08-12 | Botanical pesticide for inhibiting vegetable botrytis cinerea and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910184051A CN101623009A (en) | 2009-08-12 | 2009-08-12 | Botanical pesticide for inhibiting vegetable botrytis cinerea and application thereof |
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| CN101623009A true CN101623009A (en) | 2010-01-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910184051A Pending CN101623009A (en) | 2009-08-12 | 2009-08-12 | Botanical pesticide for inhibiting vegetable botrytis cinerea and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613082A (en) * | 2016-09-30 | 2017-05-10 | 遵义仙人岩种养殖有限公司 | Method for planting peppers |
| CN107736386A (en) * | 2017-10-17 | 2018-02-27 | 张锡林 | A kind of composition pesticide |
-
2009
- 2009-08-12 CN CN200910184051A patent/CN101623009A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 于平儒: "苦豆子等植物样品抑菌活性筛选研究", 《西北农林科技大学硕士学位论文》 * |
| 曹艳萍等: "紫穗槐叶中杀虫化学成分的分离和结构鉴定", 《化学研究与应用》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613082A (en) * | 2016-09-30 | 2017-05-10 | 遵义仙人岩种养殖有限公司 | Method for planting peppers |
| CN107736386A (en) * | 2017-10-17 | 2018-02-27 | 张锡林 | A kind of composition pesticide |
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Application publication date: 20100113 |