CN101506354B - 用胚胎干细胞制备转基因禽类的方法 - Google Patents

用胚胎干细胞制备转基因禽类的方法 Download PDF

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CN101506354B
CN101506354B CN200780031230.2A CN200780031230A CN101506354B CN 101506354 B CN101506354 B CN 101506354B CN 200780031230 A CN200780031230 A CN 200780031230A CN 101506354 B CN101506354 B CN 101506354B
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cell
stem cell
embryonic stem
chicken
embryo
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CN101506354A (zh
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I·瓦拉尔谢
L·巴塔尔
M·梅泰利
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CN200780031230.2A 2006-08-09 2007-08-09 用胚胎干细胞制备转基因禽类的方法 Expired - Fee Related CN101506354B (zh)

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Application Number Priority Date Filing Date Title
US83637806P 2006-08-09 2006-08-09
US60/836,378 2006-08-09
PCT/EP2007/058263 WO2008017704A1 (en) 2006-08-09 2007-08-09 Method of production of transgenic avian using embryonic stem cells

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CN101506354A CN101506354A (zh) 2009-08-12
CN101506354B true CN101506354B (zh) 2014-05-07

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US (2) US8962311B2 (ru)
EP (2) EP2054507A1 (ru)
JP (1) JP5405302B2 (ru)
KR (1) KR101567095B1 (ru)
CN (1) CN101506354B (ru)
AU (1) AU2007283564B2 (ru)
BR (1) BRPI0714257A8 (ru)
CA (1) CA2660035A1 (ru)
MX (1) MX2009001477A (ru)
NZ (1) NZ574678A (ru)
RU (1) RU2473688C2 (ru)
WO (1) WO2008017704A1 (ru)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2836924B1 (fr) * 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
EP1985305A1 (en) 2007-04-24 2008-10-29 Vivalis Duck embryonic derived stem cell lines for the production of viral vaccines
EP2100954A1 (en) * 2008-03-10 2009-09-16 Assistance Publique - Hopitaux de Paris Method for generating primate cardiac progenitor cells for clinical use from primate embryonic stem cells, and their applications
CA2800534C (en) 2010-06-11 2019-01-22 Regeneron Pharmaceuticals, Inc. Production of fertile xy female animals from xy es cells
EP2883951B1 (en) * 2012-06-15 2018-01-17 Kyokuto Pharmaceutical Industrial Co. Ltd Stem cell preservation medium, stem cell preservation method, and stem cell preservation system
US20140255447A1 (en) * 2013-03-05 2014-09-11 Biomune Company Production of avian embryo cells
KR101588109B1 (ko) * 2014-03-06 2016-01-25 건국대학교 산학협력단 키메라를 이용한 유도만능줄기세포 유래 체내 신경줄기세포 생산 방법
MX2016017317A (es) 2014-06-26 2017-08-24 Regeneron Pharma Metodos y composiciones para modificaciones geneticas dirigidas y metodos de uso.
US20200224156A1 (en) * 2015-09-09 2020-07-16 Cryogenetics As Method for removal of yolk
US10893666B2 (en) 2015-09-17 2021-01-19 Regeneron Pharmaceuticals, Inc. Production of fertile XY female animals by silencing of genes on the Y chromosome
US20170258495A1 (en) * 2016-02-29 2017-09-14 The Curators Of The University Of Missouri Medium supplement to increase the efficiency of oocyte maturation and embryo culture in vitro
CN108004197B (zh) * 2017-12-14 2021-06-08 浙江海洋大学 一种乌贼胚胎细胞的原代培养方法及专有试剂
CN108782458B (zh) * 2018-07-02 2022-02-11 江苏威特凯鸽业有限公司 一种改良泰深鸽的培育方法
CN110283779A (zh) * 2019-07-17 2019-09-27 吉林省农业科学院 一种鸡胚胎干细胞的分离培养方法及培养基
CN112251399B (zh) * 2020-10-21 2022-12-09 中国科学院水生生物研究所 一种用于黄鳝生殖干细胞的分离方法及培养基
CN114350599B (zh) * 2022-01-14 2024-01-19 内蒙古大学 一种增强牛胚胎干细胞的多能性和代谢活力的培养体系和培养方法
CN115088679A (zh) * 2022-07-20 2022-09-23 扬州大学 一种利用性腺细胞构建嵌合体鸡保种的方法
CN116210647A (zh) * 2023-02-03 2023-06-06 威奥福生物科技(宁波)有限公司 一种使用原始生殖细胞干细胞系扩繁白羽肉鸡的方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU623922B2 (en) 1988-08-04 1992-05-28 Amrad Operations Pty. Limited In vitro propagation of embryonic stem cells
US5162215A (en) * 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
JPH05227947A (ja) 1992-01-14 1993-09-07 Rikagaku Kenkyusho 鳥類原始生殖細胞の分離方法
AU3597093A (en) 1992-01-27 1993-09-01 Embrex Inc. Gene transfer in poultry by introduction of embryo cells (in ovo)
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
WO1994003585A1 (en) 1992-08-04 1994-02-17 Commonwealth Scientific And Industrial Research Organisation A method for maintaining embryonic stem cells and avian factor useful for same
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5589458A (en) * 1992-11-13 1996-12-31 Thomas Jefferson University Compounds that inhibit T cell proliferation and methods for using the same
FR2726003B1 (fr) 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
JPH0924997A (ja) 1995-07-11 1997-01-28 Tokico Ltd 出荷装置
US6103529A (en) 1996-10-10 2000-08-15 Life Technologies, Inc. Animal cell culture media comprising peptides derived from rice
PT841392E (pt) 1996-11-12 2004-11-30 Univ Auburn Vacina viva atenuada de neospora
CA2300336A1 (en) 1997-08-04 1999-02-11 University Of Massachusetts A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of avian embryonic germ (eg) cell lines by prolonged culturing of pgcs, use thereof for cloning and chimerization
BR9811830A (pt) 1997-08-04 2000-08-15 Univ Massachusetts Processo de cultivo que proporciona manutenção de células germinais primitivas de ave por perìodos prolongados, processo de produção de aves quiméricas, e, linhagem celular de pgc de ave
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
DE60037107T2 (de) 1999-02-11 2008-09-25 Hanmi Pharm. Co., Ltd. Pluripotente embryonale keimzellinie aus vögeln
DE19947407C2 (de) 1999-10-01 2002-08-01 Bayerische Motoren Werke Ag Datenbussystem für Kraftfahrzeuge
WO2003043414A1 (en) 2001-11-16 2003-05-30 Australian Water Management Pty Ltd A method of treating a target species of fish within a general fish population
FR2836924B1 (fr) * 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
JP2005532057A (ja) * 2002-07-09 2005-10-27 バクスター・インターナショナル・インコーポレイテッド 細胞を培養するための動物性タンパク質非含有培地
EP1500699A1 (en) 2003-07-22 2005-01-26 Vivalis Production of vaccinia virus with adherent or non adherent avian cell lines
EP1646715B1 (en) * 2003-07-22 2010-05-12 Vivalis Production of poxviruses with adherent or non adherent avian cell lines
FR2884255B1 (fr) * 2005-04-11 2010-11-05 Vivalis Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe
WO2007135133A1 (en) 2006-05-19 2007-11-29 Vivalis Avian cell lines derived from primordial germ cells useful for the production of substances of interest
EP1985305A1 (en) * 2007-04-24 2008-10-29 Vivalis Duck embryonic derived stem cell lines for the production of viral vaccines

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
B.Pain.long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morohogenetic potentialities.《development》.1996,第122卷2339-2348. *
pierrich thoraval,等.germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors.《transgenic research》.1995,第4卷369-376. *

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CA2660035A1 (en) 2008-02-14
RU2009107854A (ru) 2010-09-27
MX2009001477A (es) 2009-02-18
EP2500417A1 (en) 2012-09-19
BRPI0714257A8 (pt) 2017-05-09
JP2010500015A (ja) 2010-01-07
AU2007283564A1 (en) 2008-02-14
AU2007283564B2 (en) 2013-07-25
KR101567095B1 (ko) 2015-11-06
US8962311B2 (en) 2015-02-24
KR20090038464A (ko) 2009-04-20
RU2473688C2 (ru) 2013-01-27
NZ574678A (en) 2012-03-30
US20150225695A1 (en) 2015-08-13
WO2008017704A1 (en) 2008-02-14
JP5405302B2 (ja) 2014-02-05
EP2054507A1 (en) 2009-05-06
US20100235937A1 (en) 2010-09-16
BRPI0714257A2 (pt) 2013-12-03
CN101506354A (zh) 2009-08-12

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