CN101502269A - Compositional main pesticide containing Verticillium lecanii and Acetamiprid and pesticide - Google Patents
Compositional main pesticide containing Verticillium lecanii and Acetamiprid and pesticide Download PDFInfo
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- CN101502269A CN101502269A CNA2009100379981A CN200910037998A CN101502269A CN 101502269 A CN101502269 A CN 101502269A CN A2009100379981 A CNA2009100379981 A CN A2009100379981A CN 200910037998 A CN200910037998 A CN 200910037998A CN 101502269 A CN101502269 A CN 101502269A
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- acetamiprid
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Abstract
The present invention discloses an insect disinfestation main agent compounded by verticillum lecanii and acetamiprid and an insecticide thereof. The main agent is the mixture of verticillum lecanii and acetamiprid. The mass ratio between verticillum lecanii and acetamiprid is 100: 1.5-12. The insecticide is composed of the main agent and assistant agent. According to the weight percentage, the main agent is 22-61%, and the assistant agent is 39%-78%. After long-time infection biology research and indoor biological assay, the verticillum lecanii has strong infection insect killing effect to a plurality of insects, especially the piercing-sucking mouthparts insects. The insecticide compounded by verticillum lecanii and acetamiprid has excellent control efficiency to insects of whitefly, aphids, diamondback moth, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant the Verticillium lecanii and the composite desinsection host and the insecticide of Acetamiprid of a kind of environmental protection, pollution-free, noresidue especially.
Background technology
Verticillium lecanii (Verticillium lecanii (Zimmermann) Viegas) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Verticillium lecanii has important economic implications.Verticillium lecanii distributes extensively, Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, thrips etc. is had good control efficiency, has broad application prospects.
The novel nicotinamide insecticide of insecticidal actions such as Acetamiprid (acetamiprid) is that having of using at present tagged, stomach toxicity, infiltration and interior suction, act on the nAChR of insect nervous system postsynaptic membrane, and nerve on every side, make insect keep excited, paralysis death then.Be mainly used in suckings pest such as control homoptera pest such as leafhopper, aleyrodid, aphid, lepidoptera pest such as diamond-back moth, peach fruit borer etc., coleopteran pest such as longicorn, thrips insect such as thrips etc.; Have and good kill ovum, larvicidal activity, but that Acetamiprid shows as activity to non-target pest is not high, it is certain residual to use the back can detect on fruits and vegetables simultaneously.
Adopting the agriculture chemical compounding technology or mixing application technique is that to overcome insecticidal spectrum narrow or delay a drug-fast important method, and adopting the agricultural chemicals of sub-doses to mix with biopesticide is to improve the quick-acting of microorganism insecticide and an important method of minimizing residue of pesticide.Therefore, the Compositional type insecticide of exploitation Acetamiprid is significant.Especially utilize the composite living body biological agricultural chemicals of insect pathogenic fungus as Acetamiprid, the brand-new mechanism of action, environmentally safe, the noresidue that are different from existing chemical insecticide be can realize having, the relevant Verticillium lecanii and the compounded pesticides of Acetamiprid still found no at present.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, the Verticillium lecanii and the composite desinsection host of Acetamiprid of a kind of environmental protection, pollution-free, noresidue is provided.
Another object of the present invention provides the insecticide that is prepared by described desinsection host.
Purpose of the present invention is achieved through the following technical solutions:
Provide a kind of Verticillium lecanii and Acetamiprid composite desinsection host, described host is the mixture of Verticillium lecanii and Acetamiprid, and the mass ratio of described Verticillium lecanii and Acetamiprid is 100: 1.5～12.
In order to realize the present invention better, the mass ratio of described Verticillium lecanii and Acetamiprid is preferably 100: 1.67～and 10.
Described Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas bacterial strain VL-N6, belong to hyphomycetales, Verticillium, be located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou, bacterial strain preserving number: CCTCC No:M-208086, on June 10th, 2008 is in China's typical culture collection center preservation, in application number is 200810029163.7 patent application Verticillium lecanii had detailed description.Described Verticillium lecanii bacteria colony white is to cream-colored, and is thin cotton-shaped, and the back side is colourless to deep mixed yellow.Conidiophore is undeveloped, like nutrient hypha, Dan Sheng is arranged on it, to the bottle stalk of giving birth to or 3～4 verticillate, and the bottle stalk is very thin, and size is because of bacterial strain and cell age is different differs greatly 8.5-40 * 0.8-2.2 μ m; Conidium is oval to cylindrical, two terminal circle, and Dan Sheng is gathered into head at bottle stalk top, and size makes a variation bigger because of bacterial strain reaches, 2.3-10 * 1-2.6 μ m.
As a kind of preferred, described Verticillium lecanii conidial powder or Verticillium lecanii spore suspension.
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, as preferred scheme, the present invention provides a kind of insecticide that is prepared by described desinsection host simultaneously, described insecticide is made up of host and assistant agent, host is 22～61% by mass percentage, and assistant agent is 39～78%.
Described assistant agent is stabilizing agent, auxiliary agent and filler, and as preferably, by mass percentage, stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for compounded pesticides 18～33%.
Phosphate buffer or the paraffin oil of described stabilizing agent preferably glycerine, 0.21M; One or more mixtures among the preferred Tween-80 of described auxiliary agent, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
The compounded pesticides of described Verticillium lecanii and Acetamiprid can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art has following advantage and beneficial effect:
(1) the bacterial strain of the present invention Bemisia tabaci polypide of being infected from the area, GuangZhou, Guangdong Province separates and obtains, and is China domestic bacterial strain, is not from external introduction, can adapt to the natural environment of this locality.
(2) the present invention adopts the agricultural chemicals of sub-doses and biopesticide to be mixed, determine to obtain the mixed ratio of good insecticidal effect, reduced the consumption and the persticide residue of Acetamiprid, adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.
(3) its control efficiency of compounded pesticides of the present invention is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, Verticillium lecanii has the very strong insecticidal effect that infects to various pests especially sucking pest.The compounded pesticides of Verticillium lecanii of the present invention and Acetamiprid has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Embodiment
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, the present invention is described in further detail below in conjunction with preferred embodiment, but therefore do not limit scope of the present invention.
Be mass percent described in following examples, described Verticillium lecanii is the VL-N6 bacterial strain, the bacterial strain preserving number: CCTCC No:M-208086, preservation date are on June 10th, 2008, specifically describe to see that application number is in 200810029163.7 the patent application.
Embodiment 1:20% Verticillium lecanii acetamiprid suspending agent (dilution is 2000 times during use)
A partly is 40% Verticillium lecanii conidial suspension: 40% Verticillium lecanii conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, transfers pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: Acetamiprid (commercial) 4%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Verticillium lecanii acetamiprid suspending agent, Verticillium lecanii: the Acetamiprid mass ratio is 100: 10.The virulence test result is as shown in table 1.
Embodiment 2:50% Verticillium lecanii Acetamiprid finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, Acetamiprid 2% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Paecilomyces varioti Acetamiprid finish.Wherein, Verticillium lecanii bacterium powder: the Acetamiprid mass ratio is 100: 4.The virulence test result is as shown in table 1.
Embodiment 3:60% Verticillium lecanii Acetamiprid wetting powder (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 60%, Acetamiprid 1% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, makes 60% Verticillium lecanii Acetamiprid wetting powder.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Acetamiprid is 100: 1.67.The virulence test result is as shown in table 1.
Embodiment 4:50% Verticillium lecanii Acetamiprid finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, Acetamiprid 6% (commercial), methyl green 2%, Tween-80 10%, paraffin oil 23%, diatomite is mended to 100%, makes 50% Verticillium lecanii Acetamiprid wetting powder.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Acetamiprid is 100: 12.The virulence test result is as shown in table 1.
Embodiment 5:60% Verticillium lecanii Acetamiprid finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 60%, Acetamiprid 0.9% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Verticillium lecanii Avermectin finish.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Acetamiprid is 100: 1.5.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 Verticillium lecanii Acetamiprid is to the Toxicity Determination of Bemisia tabaci
(10
4Spore/ml, μ g/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Verticillium lecanii | y＝0.3744x+3.3240 | 0.9814 | 2.99，0.98-9.16 | 100.00 |
| Acetamiprid (behind the medicine 3 days) | y＝1.6004x+3.3185 | 0.9700 | 11.92，9.14-13.81 | |
| Example 1: Verticillium lecanii: Acetamiprid (100: 10) | y＝0.2890x+4.0537 | 0.9952 | 1.88，0.02-16.68 | 159.04 |
| Example 2: Verticillium lecanii: Acetamiprid (100: 4) | y＝0.2656x+4.1918 | 0.9996 | 1.10，0.08-13.90 | 271.82 |
| Example 3: Verticillium lecanii: Acetamiprid (100: 1.67) | y＝0.2637x+4.2023 | 0.9843 | 1.06，0.08-13.48 | 282.21 |
| Example 4: Verticillium lecanii: Acetamiprid (100: 12) | y＝0.2583x+4.2249 | 0.9933 | 1.00，0.07-13.67 | 299.00 |
| Example 5: Verticillium lecanii: Acetamiprid (100: 1.5) | y＝0.2877x+4.0387 | 0.9884 | 2.19，0.03-18.47 | 136.53 |
R
2: determination coefficient; TI: relative virus force index.
Claims (7)
1, the composite desinsection host of a kind of Verticillium lecanii and Acetamiprid is characterized in that described host is the mixture of Verticillium lecanii and Imidacloprid, and the mass ratio of described Verticillium lecanii and Imidacloprid is 100: 1.5～12.
2, according to described Verticillium lecanii of claim 1 and the composite desinsection host of Acetamiprid, the mass ratio that it is characterized in that described Verticillium lecanii and Imidacloprid is 100: 1.67～10.
3, according to claim 1 or 2 described Verticillium lecaniis and the composite desinsection host of Acetamiprid, it is characterized in that described Verticillium lecanii conidial powder or Verticillium lecanii spore suspension.
4, a kind of insecticide that is prepared by claim 1 or 2 described desinsection hosts is characterized in that being made up of host and assistant agent, and host is 22～61% by mass percentage, and assistant agent is 39～78%.
5,, it is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler according to the described insecticide of claim 4; By mass percentage, stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for compounded pesticides 18～33%.
6,, it is characterized in that described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M according to the described insecticide of claim 5; Described auxiliary agent is one or more mixtures that select among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
7,, it is characterized in that described insecticide is wetting powder, suspending agent or finish according to claim 4,5 or 6 described insecticides.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100379981A CN101502269B (en) | 2009-03-17 | 2009-03-17 | Compositional main pesticide containing Verticillium lecanii and Acetamiprid and pesticide |
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| CN2009100379981A CN101502269B (en) | 2009-03-17 | 2009-03-17 | Compositional main pesticide containing Verticillium lecanii and Acetamiprid and pesticide |
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| CN101502269A true CN101502269A (en) | 2009-08-12 |
| CN101502269B CN101502269B (en) | 2011-08-17 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532582A (en) * | 2015-12-28 | 2016-05-04 | 北京市农林科学院 | Indoor bioassay method for bemisia tabaci imagoes |
| CN105901034A (en) * | 2016-04-29 | 2016-08-31 | 山东胜伟园林科技有限公司 | Verticillium containing biological pesticide for prevention and control of peanut diseases and pests and preparation method of verticillium containing biological pesticide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100569080C (en) * | 2007-11-20 | 2009-12-16 | 华南农业大学 | The compounded pesticides of paecilomyces fumosoroseus and Imidacloprid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532582A (en) * | 2015-12-28 | 2016-05-04 | 北京市农林科学院 | Indoor bioassay method for bemisia tabaci imagoes |
| CN105901034A (en) * | 2016-04-29 | 2016-08-31 | 山东胜伟园林科技有限公司 | Verticillium containing biological pesticide for prevention and control of peanut diseases and pests and preparation method of verticillium containing biological pesticide |
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Granted publication date: 20110817 Termination date: 20170317 |