CN101496574A - Closed type solid fermentation reaction device - Google Patents

Closed type solid fermentation reaction device Download PDF

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Publication number
CN101496574A
CN101496574A CNA2009100471297A CN200910047129A CN101496574A CN 101496574 A CN101496574 A CN 101496574A CN A2009100471297 A CNA2009100471297 A CN A2009100471297A CN 200910047129 A CN200910047129 A CN 200910047129A CN 101496574 A CN101496574 A CN 101496574A
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China
Prior art keywords
closed container
humidifier
gas
temperature
solid fermentation
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CNA2009100471297A
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CN101496574B (en
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张晞
许学书
谢静莉
余东辉
夏月兰
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East China University of Science and Technology
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East China University of Science and Technology
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Abstract

The invention relates to a closed type solid fermentation reactor, which belongs to the field of solid state fermentation reactors and comprises a closed container and an air intake system, wherein the air intake system is connected with the closed container, adds water and introduces moisture-keeping gas into the closed container to perform solid fermentation in the process of fermentation. The air intake system comprises a humidifier and a water storage bottle to introduce moisture-keeping air and moisture into the closed container respectively, and a control system to perform time control on the reactor. The closed container is a cylindrical container of which the bottom is provided with an air chamber. The air chamber is separated from the main part of the container by an air distribution plate; and the inside of the closed container is provided with a stirring device of which the top is provided with a moisture atomizing device. The humidifier can well control the temperature and the humidity of the gas passed into the reactor. The reactor is operated closely and controls the temperature therein in the process of the fermentation through the temperature and the flow of the gas passed therein. With the stirring device, the moisture can be added in the process of the fermentation, the fermentation parameters can be controlled well, and the quality and the yield of the fermentation can be improved.

Description

Closed type solid fermentation reaction device
Technical field
The invention belongs to the solid state fermentation field, particularly a kind of systematization solid-state fermentation reactor.
Background technology
Leaven cultivating equipment plays a part very important in making leaven of soy sauce technology.Making leaven of soy sauce still mainly adopts bamboo plaque mode at present, and this mode mechanization degree is low, and labour intensity is big, and can not reach sterile working, particularly runs into the yellow plum sky, and the sauce song is easy to microbiological contamination.Therefore the soy sauce brewing industry is badly in need of a kind of mechanization intensity height, the equipment of closed-loop operation.Occurred some brewing equipments now, but these equipment manufacturing cost costlinesses are difficult to large-scale promotion.A topmost problem is exactly a heat dissipation problem in solid fermentation, because the solid substrate thermal conductance is poor, causes heat convection current and heat transfer efficiency low, on the heat production peak, bed temperature sharply rises, and thermograde occurs, must reduce the appearance of thermograde during the fermentation as far as possible.General the mode of evaporation is controlled temperature by cross-ventilation, but must consider the effect of cross-ventilation to water evaporates, prevents the moisture excessive loss.
Summary of the invention
The object of the present invention is to provide a kind of closed type solid fermentation reaction device, this device is airtight fully, can realize sterile working, guarantees the security of fermented product.
For reaching above purpose, solution of the present invention is:
A kind of solid fermentation reaction device, it comprises closed container, gas handling system, and gas handling system connects closed container, and gas handling system is added moisture and the feeding gas of preserving moisture to closed container during the fermentation and is carried out solid fermentation.
Further, it also comprises control system, and control system is carried out time control to reaction unit.
Described closed container comprises agitating device, atomizer, dispersion plate, temperature electrode, humidity electrode, central shaft, central shaft is connected by belt with the motor of closed container outside, central shaft is provided with agitating device makes motor rotate drive agitating device rotation stirring, atomizer is located at the closed container top, closed container is separated out an air chamber by dispersion plate in its bottom, and air chamber is provided with air inlet.
Described closed container is the stainless steel column-shaped barrel, and a side also is provided with the visor of glass.
Described closed container outer wall can also be established insulation water-bath or annular heating tape.
Described temperature electrode is three, lays respectively at the top and measures round top gas phase temperature, solid material temperature and be positioned at air chamber mensuration intake air temperature; The humidity electrode then is positioned at the tank body top and measures gas phase humidity.
Described agitating device is the helical ribbon blade, is welded by two width annulus identical with diameter, and the ribbon width is 1/10 of a barrel diameter, is fixed on central shaft by a head screw.
Described gas handling system, comprise air compressor, filter membrane, gas bleeder valve, gas flowmeter, operated pneumatic valve, water storage bottle and humidifier, air compressor connects gas flowmeter and connects humidifier and water storage bottle respectively by operated pneumatic valve, the humidifier air inlet that is connected with the air chamber of closed container, the water storage bottle into water that is connected with the atomizer of closed container; Described filter membrane is two, and first filter membrane and second filter membrane are located at the gas flowmeter both sides respectively, and gas bleeder valve is two also, is located between filter membrane and the gas flowmeter.
Described humidifier top is provided with cover plate, is provided with the thermocouple, air inlet pipe, gas outlet, the temperature electrode that pass cover plate in the humidifier.The air inlet pipe bottom is a ring-type, and has aperture downwards; Humidifier is provided with filler and discharge outlet, and the filler is connected with the water storage bottle that adds water; Humidifier also is provided with the liquid level gauge of measuring inner liquid level.
Described humidifier is the cylindrical stainless steel cylindrical shell that certain liquid level is housed, described liquid is water, water is provided by the liquid level difference by the water storage bottle, its water temperature is stabilized in setting value by the two-way temperature controller of heating cooling, and humidifier top cover plate seals by flange and by ring-type tetrafluoroethene band.
In the closed container, central shaft with adopt mechanical dynamic seal on upper cover plate is connected, in needs sealing place the tetrafluoroethene material is arranged all.After tank body and material sterilization, use liquid state fermentation pyrosphere method commonly used during inoculation, insert the solid seed.Adopt the sterilization of humidifier wet-hot steam method for the sterilization of humidifier and pipeline.In the time of need adding moisture to material, adopt after filtration that the aseptic gas of membrane filtration is pressed into tank body with the sterilized water in the water storage bottle, and be sprayed on the material, open simultaneously and stir, moisture is mixed.In whole solid material sweat, this device can be realized sterile working, guarantees fermented quality.
Owing to adopted such scheme, the present invention has following characteristics: the temperature of the present invention's energy better controlled sweat, by inlet air control bed temperature, owing to added the distinctive stirring system in the solid environment, can add moisture during the fermentation voluntarily, prevent because the moisture excessive loss that forced convertion ventilation heat radiation causes.This reactor closed-loop operation can be with the temperature of solid fermentation, moisture, and ventilation well is coupled together, and reaches good ferment effect.
Description of drawings
Fig. 1 is the solid fermentation reaction device schematic diagram.
Fig. 2 is a solid fermentation reactor air-channel system schematic diagram.
Fig. 3 is the humidifier structural representation.
The specific embodiment
The present invention is further illustrated below in conjunction with the accompanying drawing illustrated embodiment.
This invention solid fermentation reactor comprises closed container, gas handling system.
Belt 1 couples together tank body central shaft 13 and motor 14, adds that electric current passes through speed change, and potentiometer makes speed of agitator be controlled at 0-15rpm after regulating, and satisfies the slow-revving requirement of der Pilz solid fermentation.
Paddle in the closed container is a helical ribbon blade 6, and it is to be welded by two width annulus identical with diameter.The ribbon width is 1/10 of a barrel diameter, is fixed on the central shaft 13 by a head screw, and when motor 14 rotated, stirrer paddle rotated, and solid material is overturn from the bottom up along blade, and falls from blade centre and tank skin, and material can well be mixed.
Closed container is the stainless steel column-shaped barrel, and a side is provided with the visor 7 of glass.Visor is mainly in order to observe the microbial growth situation during the fermentation, the growth of mycelia and the formation of spore.Closed container makes the bottom form an air chamber 11 by dispersion plate 8.Gas enters in air chamber 11 from bottom air inlet 10 and enters the fermentation tank main part through behind the dispersion plate 8, and then the gas vent 5 from top flows out.Being equipped with three temperature electrodes in the closed container, is respectively the gas phase temperature electrode 2 that is used to measure round top gas phase temperature, measures the solid-state temperature electrode 4 of solid material temperature and the gas phase temperature electrode 9 of the air chamber temperature of measuring air inlet.A humidity electrode 3 is positioned at the tank body top and measures gas phase humidity.The closed container outer wall can add insulation water-bath or annular heating tape, at the fermentation initial stage, can promote spore germination by insulation water-bath or heat that annular heating tape provided.
The closed container top also has an atomizer 12.When needs when tank body adds moisture, second operated pneumatic valve 20 that leads to one tunnel gas of humidifier 23 is closed, simultaneously, open first operated pneumatic valve 19 of water injector, gas leads to the first water storage bottle 22 presses to fermentation tank with the sterilized water in the bottle, by atomizer 12, sprays moisture.Meanwhile open and stir, make mixing of materials even.
In the gas handling system, gas filters by first filter membrane 16 after being compressed by air compressor 15, regulate flow velocity through mass air flow sensor 18 again, further filter through second filter membrane 17 again, through behind humidifier 23 heating (cooling) humidifications, enter closed container bottom air chamber 11 then.When not needing to add moisture, second operated pneumatic valve 20 of humidifier one tunnel often is in to be opened the stage.Be in security consideration, respectively be provided with gas bleeder valve between gas flowmeter 18 and first filter membrane 16, second filter membrane 17, in whole fermentation process, air compressor machine 15 is under the operating state always, when needing to stop to ventilate when sampling or on the technology, at this moment need to open air-outlet valve and lose heart.Humidifier 23 is for being equipped with the cylindrical stainless steel cylindrical shell of certain liquid level.Water temperature in the humidifier 23 is stabilized in setting value by the two-way temperature controller of heating cooling.Thermocouple 24 heating are arranged in the humidifier 23, when temperature surpasses setting value, open cooling, colling end is connected with a fan.The gas that enters humidifier 23 enters the humidifier bottom by air inlet pipe 25, and the bottom inflow pipe is a ring-type, and has aperture downwards.Gas is flowed out by the gas outlet 26 at top by the liquid level of humidifier certain altitude, has a temperature electrode 27 to be used to measure the temperature of humidifier internal water in the middle of the humidifier 23.Humidifier 23 top cover plates seal by flange 28 and by ring-type tetrafluoroethene band.Water is to be provided by the liquid level difference by the second water storage bottle 21 in the humidifier 23, flows to from humidifier water inlet 30.When fermentation ends, humidifier 23 internal water should in time be discharged by discharge outlet 31.Two liquid level gauges 29 can be observed inner liquid level about humidifier 23 1 sides were provided with in addition, prevented that water level is low excessively, influences the humidification effect.The humidifier temperature can be controlled with two-way control temperature controller, mainly is for enough temperature and humidities are provided to gas, by regulating water temperature in the humidifier, hydrothermal exchange when gas flows through humidifier and in the humidifier.Make air obtain heating or cooling, thereby the temperature of air is controlled.In addition, when air passed through humidifier, the moisture of air was able to saturated, becomes the gas of preserving moisture.Forced Air Convection was to the evaporation of the moisture of solid matrix in the reactor when this helped reducing fermentation, and the increase of bed pressure drop and bed shrink, and prevent the appearance in tangible circulation of air slit, influence growth of microorganism.
Time and cycle that control system control is stirred, the time of water spray and cycle.This realizes by the time relay, generally pre-sets duration and interval that the time relay is opened, controls the running of motor, thereby can control the opening time and the interval of stirring.Water spraying time and interval also are like this.
The temperature and the flow-control fermentation tank body temperature degree of the gas of reactor of the present invention by entering closed container.When growth of microorganism is vigorous in the tank body, can produce a large amount of heats.The heat that removes by tank skin cooling is limited, therefore considers the main mode that convection current and water evaporates by air remove as heat.By temperature and the flow-control temperature that enters tank body gas.When but air mass flow was big, the water evaporates of solid material was serious, and is very limited by the initial moisture that strengthens solid material.Therefore the present invention adopts in the way method of adding moisture to tank body to add moisture, and has added stirring system and make moisture distribution even.
This device is airtight fully, after the device sterilization, is difficult for microbiological contamination.Reach airtight aseptic purpose.
Example
At first the fermentation solid material is placed in the solid reactor, with reactor and sterilized water water storage bottle in 121 ℃ of sterilizations of autoclave 30min.Add sterilized water to certain altitude to humidifier.Open the humidifier heater switch, sterilized water is heated to boiling and produces steam, continue heating 15min, make humidifier and gas pipeline reach sterilization effect.Behind the 15min, close heater switch, regulate the humidifier temperature to fermentation temperature, the humidifier colling end is connected with fan, and the humidifier cooling is opened, and opens air compressor simultaneously, makes gas pass through humidifier, quickens the cooling of humidifier.Treat the humidifier temperature stabilization when fermentation temperature, the humidifier gas outlet be connected with the closed container air inlet that gas enters the solid fermentation reactor.Open the inoculation mouth, under aseptic technique, add the solid seed.Open and stir, seed and solid material are mixed.Behind the 15min, close stirring.Begin fermentation.
In the microbial fermentation stage, show the fermentation situation by the temperature and humidity electrode.Prescribe a time limit when temperature surpasses on the microbial fermentation, strengthen ventilation flow rate, reduce the humidifier temperature,, can open stirring and reduce temperature if thalline self allows stirring.When humidity is reduced to certain value, open water injector.Close a road the gas operated pneumatic valve that leads to humidifier, simultaneously, open the operated pneumatic valve of water injector, gas leads to the water storage bottle presses to tank body with the sterilized water in the bottle, by atomizer, sprays moisture.Meanwhile open agitating device, make mixing of materials even.
Time of stirring and spraying water and cycle can the manual or controls automatically by the time relay.
The above-mentioned description to embodiment is can understand and apply the invention for ease of those skilled in the art.The person skilled in the art obviously can easily make various modifications to these embodiment, and needn't pass through performing creative labour being applied in the General Principle of this explanation among other embodiment.Therefore, the invention is not restricted to the embodiment here, those skilled in the art should be within protection scope of the present invention for improvement and modification that the present invention makes according to announcement of the present invention.

Claims (10)

1, a kind of solid fermentation reaction device is characterized in that: it comprises closed container, gas handling system, and gas handling system connects closed container, and gas handling system is added moisture and the feeding gas of preserving moisture to closed container during the fermentation and carried out solid fermentation.
2, solid fermentation reaction device as claimed in claim 1 is characterized in that: it also comprises control system, and control system is carried out time control to reaction unit.
3, solid fermentation reaction device as claimed in claim 1, it is characterized in that: described closed container comprises agitating device, atomizer, dispersion plate, temperature electrode, humidity electrode, central shaft, central shaft is connected by belt with the motor of closed container outside, central shaft is provided with agitating device makes motor rotate drive agitating device rotation stirring, atomizer is located at the closed container top, closed container is separated out an air chamber by dispersion plate in its bottom, and air chamber is provided with air inlet.
4, solid fermentation reaction device as claimed in claim 2 is characterized in that: described closed container is the stainless steel column-shaped barrel, and a side also is provided with the visor of glass.
5, solid fermentation reaction device as claimed in claim 2 is characterized in that: described closed container outer wall also is provided with insulation water-bath or annular heating tape.
6, solid fermentation reaction device as claimed in claim 2 is characterized in that: described temperature electrode is three, lays respectively at the top and measures round top gas phase temperature, solid material temperature and be positioned at air chamber mensuration intake air temperature; The humidity electrode then is positioned at the tank body top and measures gas phase humidity.
7, solid fermentation reaction device as claimed in claim 2, it is characterized in that: described agitating device is the helical ribbon blade, be welded by two width annulus identical with diameter, the ribbon width is 1/10 of a barrel diameter, is fixed on central shaft by a head screw.
8, solid fermentation reaction device as claimed in claim 1, it is characterized in that: described gas handling system, comprise air compressor, filter membrane, gas bleeder valve, gas flowmeter, operated pneumatic valve, water storage bottle and humidifier, air compressor connects gas flowmeter and the operated pneumatic valve by separately connects humidifier and water storage bottle respectively, the humidifier air inlet that is connected with the air chamber of closed container, the water storage bottle into water that is connected with the atomizer of closed container; Described filter membrane is two, and first filter membrane and second filter membrane are located at the gas flowmeter both sides respectively, and gas bleeder valve is two also, is located between filter membrane and the gas flowmeter.
9, solid fermentation reaction device as claimed in claim 8, it is characterized in that: described humidifier top is provided with cover plate, be provided with the thermocouple, air inlet pipe, gas outlet, the temperature electrode that pass cover plate in the humidifier, the air inlet pipe bottom is a ring-type, and has aperture downwards; Humidifier is provided with filler and discharge outlet, and the filler is connected with the water storage bottle that adds water; Humidifier also is provided with the liquid level gauge of measuring inner liquid level.
10, solid fermentation reaction device as claimed in claim 9, it is characterized in that: described humidifier is the cylindrical stainless steel cylindrical shell that liquid level is housed, described liquid is water, water is provided by the liquid level difference by the water storage bottle, its water temperature is stabilized in setting value by the two-way temperature controller of heating cooling, and humidifier top cover plate seals by flange and by ring-type tetrafluoroethene band.
CN2009100471297A 2009-03-06 2009-03-06 Closed type solid fermentation reaction device Expired - Fee Related CN101496574B (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102199521A (en) * 2011-04-07 2011-09-28 华东理工大学 Enclosed solid state fermentation bioreactor
CN102220223A (en) * 2011-03-25 2011-10-19 重庆良酩科技发展有限公司 Solid-state fermentation material processing device
CN102499190A (en) * 2011-11-23 2012-06-20 广东省昆虫研究所 Ventilating and stirring type Hermetia illucens bioprocessor
CN102925336A (en) * 2012-10-31 2013-02-13 中国科学院过程工程研究所 Chained lifting solid fermentation tank and fermentation method
CN103695294A (en) * 2013-12-20 2014-04-02 中粮生物化学(安徽)股份有限公司 Microorganism solid-state culture system
CN105505771A (en) * 2016-02-04 2016-04-20 山东百德生物科技有限公司 Multifunctional solid-phase saccharification and fermentation equipment
CN106190831A (en) * 2016-08-17 2016-12-07 金乐仙 A kind of normal pressure pure oxygen reactor and method
CN107935654A (en) * 2017-11-30 2018-04-20 重庆蕊福农食用菌种植有限公司 Zymolysis Equipment for bacteria residue manufacture organic fertilizer
CN114106988A (en) * 2012-10-26 2022-03-01 麻省理工学院 Humidity control in chemical reactors

Family Cites Families (3)

* Cited by examiner, † Cited by third party
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CN2229458Y (en) * 1995-03-31 1996-06-19 清华紫光(集团)总公司 Belt solid fermentation reaction apparatus
CN1142533A (en) * 1995-08-08 1997-02-12 山东省科学院山科饲料酵母厂 Automatic closed solid fermentation reactor
CN100558876C (en) * 2006-12-26 2009-11-11 沈阳农业大学 Automotically feeding-discharging sealing type solid fermenting tank and zymotechnique thereof

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102220223B (en) * 2011-03-25 2014-08-06 重庆良酩科技发展有限公司 Solid-state fermentation material processing device
CN102220223A (en) * 2011-03-25 2011-10-19 重庆良酩科技发展有限公司 Solid-state fermentation material processing device
CN102199521A (en) * 2011-04-07 2011-09-28 华东理工大学 Enclosed solid state fermentation bioreactor
CN102499190A (en) * 2011-11-23 2012-06-20 广东省昆虫研究所 Ventilating and stirring type Hermetia illucens bioprocessor
CN114106988A (en) * 2012-10-26 2022-03-01 麻省理工学院 Humidity control in chemical reactors
CN102925336A (en) * 2012-10-31 2013-02-13 中国科学院过程工程研究所 Chained lifting solid fermentation tank and fermentation method
CN102925336B (en) * 2012-10-31 2014-06-04 中国科学院过程工程研究所 Chained lifting solid fermentation tank and fermentation method
CN103695294B (en) * 2013-12-20 2015-08-19 中粮生物化学(安徽)股份有限公司 Microorganism solid-state culture system
CN103695294A (en) * 2013-12-20 2014-04-02 中粮生物化学(安徽)股份有限公司 Microorganism solid-state culture system
CN105505771A (en) * 2016-02-04 2016-04-20 山东百德生物科技有限公司 Multifunctional solid-phase saccharification and fermentation equipment
CN106190831A (en) * 2016-08-17 2016-12-07 金乐仙 A kind of normal pressure pure oxygen reactor and method
CN107935654A (en) * 2017-11-30 2018-04-20 重庆蕊福农食用菌种植有限公司 Zymolysis Equipment for bacteria residue manufacture organic fertilizer
CN107935654B (en) * 2017-11-30 2020-09-29 重庆蕊福农食用菌种植有限公司 Fermentation equipment for producing organic fertilizer by using mushroom dregs

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