CN103695294B - Microorganism solid-state culture system - Google Patents
Microorganism solid-state culture system Download PDFInfo
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- CN103695294B CN103695294B CN201310718455.2A CN201310718455A CN103695294B CN 103695294 B CN103695294 B CN 103695294B CN 201310718455 A CN201310718455 A CN 201310718455A CN 103695294 B CN103695294 B CN 103695294B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses a kind of Microorganism solid-state culture system, this Microorganism solid-state culture system comprises microbe in solid state culture apparatus (100) and fluid source (200), this microbe in solid state culture apparatus comprises casing (110), first enters pipe (120) and vent pipe (130), described first enters pipe (120) is communicated with described fluid source (200), wherein, described microbe in solid state culture apparatus also comprises the sieve plate (140) with sieve aperture, described sieve plate (140) is arranged in described casing (110), and the internal space of this casing (110) is divided into two-layer up and down, described first enters pipe (120) communicates with the lower floor of described casing (110), described vent pipe (130) is by described casing (110) and ft connection.By after the bottom of casing arranges sieve plate, sterile air or water vapor is passed in the bottom casing of body, sterile air or water vapor rise and through the material be deposited on sieve plate, thus make to have less temperature head and psychrometric difference between material the upper and lower.
Description
Technical field
The present invention relates to the culture device of microorganism, particularly, relate to a kind of Microorganism solid-state culture system.
Background technology
Solid state fermentation is one of human use's microorganisms producing product technology with the longest history, and the time-honored brewing industry of China is the Typical Representative of solid state fermentation.Solid state fermentation contains water-fast solid, a small amount of moisture and air, the thermal conductance that microorganism generates causes moisture evaporation, fermentation system is made to have the solid uneven three-phase of vapour-liquid, there is serious concentration gradient and heat transfer, mass transfer difficulty, be difficult to control pH like this, water activity, optimal reaction temperature etc., output is declined greatly, the most important condition of especially modern fermentation technique is purebred cultivation, other microorganism natural is not allowed to enter, cause living contaminants, add the requirement of modern industry to extensive intensive manufacture, the production application of solid state fermentation is stayed cool, almost be ostracised outside modern industry.But in recent years, day by day serious due to energy dilemma and environmental problem, solid-state fermentation technology causes the interest of people again, and earth-shaking change has appearred in the research in solid state fermentation field.
Disclose a kind of automatic rotary microbial spore incubator in CN102212472A, this automatic rotary microbial spore incubator mainly comprises incubator, sterile air adds pipe and steam adds pipe.When culturing micro-organisms, material is added in incubator, then add pipe by sterile air heating tube and steam and pass into sterile air and steam in incubator.But in this automatic rotary microbial spore incubator, the bottom of solid accumulation incubator, makes the gradient difference of material bed upper and lower temperature and humidity comparatively large, is unfavorable for the growth of microbial spore.
Therefore, the gradient difference how reducing temperature and humidity up and down material bed in microbe in solid state culture apparatus becomes this area technical problem urgently to be resolved hurrily.
Summary of the invention
The object of this invention is to provide a kind of Microorganism solid-state culture system, the material bed upper and lower temperature in this Microorganism solid-state culture system and humidity more even.
To achieve these goals, the invention provides a kind of Microorganism solid-state culture system, this Microorganism solid-state culture system comprises microbe in solid state culture apparatus and fluid source, this microbe in solid state culture apparatus comprises casing, first enters pipe and vent pipe, described first enters pipe is communicated with described fluid source, wherein, described microbe in solid state culture apparatus also comprises the sieve plate with sieve aperture, described sieve plate is arranged in described casing, and the internal space of this casing is divided into two-layer up and down, described first enters pipe communicates with the lower floor of described casing, described vent pipe is by described casing and ft connection.
Preferably, described casing comprises box main body and chuck, described sieve plate is arranged on the diapire of described box main body, the bottom of described box main body is arranged in described chuck, and there is interval between the outer wall of described box main body and this chuck, described first enter pipe and described interval communicate, the upper end of described chuck and the outside surface of described box main body are tightly connected.
Preferably, described microbe in solid state culture apparatus also comprises heating unit, and this heating unit is arranged in described interval.
Preferably, described sieve plate is formed as tabular.
Preferably, described fluid source comprises vapour source and air source, described microbe in solid state culture apparatus comprises supervisor, vapour pipe and blast main, it is outside that this supervisor is positioned at described casing, described first enters pipe is connected with this supervisor in the downstream of described supervisor, and one end of described vapour pipe is communicated with described vapour source, and the other end is communicated with described supervisor, one end of described blast main is communicated with described air source, and the other end is communicated with supervisor.
Preferably, described Microorganism solid-state culture system also comprises air modifier and air filter, the side of described air filter is communicated with described air source, opposite side is communicated with described air modifier, described blast main comprises dry air pipe, wet air pipe pipe and mixing tube, described dry air pipe is communicated with between described air filter and described air modifier, described wet air pipe is communicated with in described air modifier downstream, described dry air pipe and described wet air pipe are provided with first flow control valve, described dry air pipe and described wet air pipe meet at described mixing tube, this mixing tube is communicated with described supervisor.
Preferably, described mixing tube is provided with second control valve.
Preferably, described microbe in solid state culture apparatus also comprises the first wet bulb thermometer, and this first wet bulb thermometer is arranged on described mixing tube, and is positioned at described steam pipe upstream.
Preferably, this Microorganism solid-state culture system also comprises the first controller, described first flow control valve is magnetic valve, described first controller is electrically connected with described first wet bulb thermometer and described first flow control valve, to pass through first flow control valve described in the humid control measured by described first wet bulb thermometer.
Preferably, described dry air pipe is provided with switch-valve.
Preferably, described first enter pipe and comprise and first enter first of pipe two ends at this and enter tube stub and be connected to these two first and enter and enter flexible pipe between tube stub.
Preferably, this microbe in solid state culture apparatus also comprises second and enters pipe, and this second one end entering pipe is communicated with the upper strata of described casing, and the other end is communicated with described supervisor.
Preferably, one end of described vent pipe is fixed on the sidewall of described casing, and the other end extends to the outside of described casing, and the sidewall of described vent pipe is provided with multiple through hole, and the described the other end is provided with venting port.
Preferably, the outside of described vent pipe is coated with screen cloth.
Preferably, described microbe in solid state culture apparatus comprises discharge extension tube, and this discharge extension tube in the outside of described casing, and is communicated with described vent pipe, and described discharge extension tube is provided with the second wet bulb thermometer.
Preferably, described discharge extension tube is above-mentioned is provided with under meter, and this under meter is electrically connected with described first flow control valve and described first controller.
Preferably, described Microorganism solid-state culture system also comprises power set, and this power set comprises the rotation axis be fixedly connected with the sidewall of described casing, and this rotation axis can around own axis.
Preferably, the described power set second controller that comprises pulse encoder and be electrically connected with this pulse encoder.
Preferably, also comprise temperature sensor, this temperature sensor is arranged on the bottom of described casing.
Preferably, described Microorganism solid-state culture system also comprises the brush be arranged on described rotation axis and the 3rd controller be electrically connected with this brush, described rotation axis can rotate relative to described brush, described temperature sensor is electrically connected with described brush, and described 3rd controller is electrically connected with described heating unit
By after the bottom of casing arranges sieve plate, sterile air or water vapor is passed in the bottom casing of body, sterile air or water vapor rise and through the material be deposited on sieve plate, thus make to have less temperature head and psychrometric difference between material the upper and lower.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and forms a part for specification sheets, is used from explanation the present invention, but is not construed as limiting the invention with embodiment one below.In the accompanying drawings:
Fig. 1 is the schematic diagram of Microorganism solid-state culture system provided by the invention;
Fig. 2 is the schematic diagram of the microbe in solid state culture apparatus in the Microorganism solid-state culture system shown in Fig. 1;
It is the vertical view of the preferred implementation of Microorganism solid-state culture system provided by the present invention with Fig. 3.
Description of reference numerals
100 microbe in solid state culture apparatus 110 casings
111 box main body 112 chucks
113 intervals 120 first enter pipe
121 first enter tube stub 122 enters flexible pipe
123 second enter pipe 130 vent pipe
Extension tube discharged by 131 screen clothes 132
133 second wet bulb thermometer 134 under meters
140 sieve plate 141 temperature sensors
142 brushes 150 are responsible for
151 first wet bulb thermometer 160 vapour pipes
170 blast main 171 dry air pipes
172 wet air pipe 173 first flow control valves
174 mixing tube 175 switch-valves
200 fluid source 210 vapour sources
220 air source 300 air modifiers
400 air filter 500 power set
510 rotation axis 520 pulse encoders
176 second control valves
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
As shown in Figure 1 to Figure 3, the invention provides a kind of Microorganism solid-state culture system, this Microorganism solid-state culture system comprises microbe in solid state culture apparatus 100 and fluid source 200, this microbe in solid state culture apparatus comprises casing 110, first enters pipe 120 and vent pipe 130, first enters pipe 120 is communicated with fluid source 200, wherein, microbe in solid state culture apparatus also comprises the sieve plate 140 with sieve aperture, sieve plate 140 is arranged in casing 110, and the internal space of this casing 110 is divided into two-layer up and down, first enters pipe 120 communicates with the lower floor of casing 110, vent pipe 130 is by casing 110 and ft connection.
Fluid in fluid source 200 comprises sterile air and water vapor, water vapor or sterile air can enter by first the lower floor that pipe 120 enters casing 110, then enter in material by the sieve aperture of sieve plate 140, along with water vapor or sterile air continue pass into, water vapor or sterile air pass from the bottom of material the upper strata that material arrives casing 110, thus all materials can be contacted fully with water vapor or sterile air, reduce temperature head and the psychrometric difference of material upper strata and material lower floor.
When water vapor or sterile air are through sieve plate 140 and material, can be discharged in casing 110 by vent pipe 130 after entering the upper strata of casing 110.
In the present invention, the internal space of casing 110 is divided into two-layer up and down by the inside that sieve plate 140 can be set directly at casing 110.Preferably, casing 110 comprises box main body 111 and chuck 112, sieve plate 140 is arranged on the diapire of box main body 111, the bottom of box main body 111 is arranged in chuck 112, and there is interval 113 between the outer wall of box main body 111 and this chuck 112, first enters pipe 120 and interval 113 communicates, and the upper end of chuck 112 and the outside surface of box main body 111 are tightly connected.Should be understood that, " interval 113 " described herein is above described " lower floor of casing 110 ".
Water vapor that fluid source 200 provides by pipe 120 can be entered or sterile air passes in interval 113 by first.Water vapor or sterile air are entered in material by the sieve aperture of sieve plate 140.The advantage arranging chuck 112 is, after water vapor or sterile air being passed in chuck 112, is conducive to being incubated box main body 111, thus is conducive to the microbial growth in box main body 111.
The advantage that the upper end of chuck 112 and the outside surface of box main body 111 are tightly connected is, pass into the outside that sterile air in chuck 112 or water vapour can not flow to casing 110, and the air of outside also can not enter the inside of chuck 112.
In the present invention, metallic substance (such as, steel) can be utilized to make chuck 112, and chuck 112 can be made into spherical shell shape, cover on the outside of the bottom of box main body 111.The mode of welding can be utilized to be fixed on box main body 111 by chuck 112.
In the present invention, sieve plate 40 can be formed as the diapire of box main body 11, that is, the diapire of box main body 11 is processed multiple sieve aperture, thus makes the diapire of box main body 11 be formed as sieve plate 40.In addition, a through hole can also be processed on the diapire of box main body 11, then the sieve plate 40 with sieve aperture is arranged in described through hole.
In order to the homo(io)thermism in guard box main body 111, preferably, microbe in solid state culture apparatus 100 can also comprise heating unit, and this heating unit is arranged in described interval 113.Described heating unit can be electric heater unit, also can be other forms of heating unit.When temperature is too low in interval 113, described heating unit can be started, when temperature is too high in interval 113, then can stop described heating unit.
Preferably, sieve plate 140 is formed as tabular.Sieve plate 140 is set to tabular and is not only convenient to processing and manufacturing, but also a large amount of materials can be placed on flat sieve plate 140.
As mentioned above, when culturing micro-organisms, need in microbial cultivation device, pass into water vapor and sterile air.The major function passing into water vapor ensures to have sufficient moisture and high-temperature sterilization in material (mainly substratum), the major function passing into sterile air ensures the oxygen required for microorganism growth, and take away the heat and carbonic acid gas that produce in microorganism growth process.Same pipe first backward first can be utilized to enter pipe 20 and to pass into sterile air and water vapor.
For the ease of operation, preferably, fluid source 200 comprises vapour source 210 and air source 220, microbe in solid state culture apparatus 100 comprises supervisor 150, vapour pipe 160 and blast main 170, and it is outside that this supervisor 150 is positioned at casing 110, and first enters pipe 120 is connected with this supervisor 150 in the downstream of supervisor 150, one end of vapour pipe 160 is communicated with vapour source 210, the other end is communicated with supervisor 150, and one end of blast main 170 is communicated with air source 220, and the other end is communicated with supervisor 150.The water vapor that vapour source 210 provides enters in supervisor 150 by vapour pipe 160, and the air that air source 220 provides is entered in supervisor 150 by blast main 170.Water vapor in supervisor 150 or air are passed in interval 113.
One skilled in the art will appreciate that when culturing micro-organisms, and non-concurrent pass into water vapor and sterile air in casing 110.But first in casing 110, pass into water vapor, thus carry out High Temperature Sterilization, then in casing 110, pass into sterile air.
Important factor when humidity in casing 110 is culturing micro-organisms.Casing 110 humidity is too small, and the moisture in material easily evaporates, thus material is become dry and affects microbial growth; If the humidity in casing 110 is excessive, then can affects the oxygen level in casing 110, cause environmental hypoxia, but also material surface can be made because of moisture in air condensation to become wet, affect microbial growth or contaminating microorganisms.
In order to obtain sterile air, first to filtering air, obtaining aseptic dry air, in order to obtain aseptic wet air, also will be humidified to aseptic dry air.Therefore, described Microorganism solid-state culture system also comprises air modifier 300 and air filter 400, and the side of air filter 400 is communicated with air source 220, and opposite side is communicated with air modifier 300.
In order to ensure the humidity of the material in casing 110, need to pass into aseptic wet air in casing 110.The air that air source 220 provides obtains aseptic dry air after air filter 400, is passed in air modifier 300 by aseptic dry air and can obtain aseptic wet air.The atmospheric moisture in casing 110 can be controlled by the humidity controlling aseptic wet air.
For the ease of controlling the atmospheric moisture in casing 110, preferably, blast main 170 can comprise dry air pipe 171, wet air pipe 172 is managed, the empty vapour that air source 220 provides directly enters in casing 110 by dry air pipe 171 through a part for the sterile air that air filter 400 obtains, another part is introduced into air modifier 300 and carries out the aseptic wet air of humidification acquisition, and then enter wet air pipe 172, and entered in casing 110 by wet air pipe 172.
In order to be convenient to control the atmospheric moisture in casing 10 further, blast main 170 can also comprise mixing tube 174, dry air pipe 171 is communicated with between air filter 400 and air modifier 300, wet air pipe 172 is communicated with in air modifier 300 downstream, dry air pipe 171 and wet air pipe 172 are provided with first flow control valve 173, dry air pipe 171 and wet air pipe 172 meet at mixing tube 174, and this mixing tube 174 is communicated with supervisor 150.
Before aseptic dry air and aseptic wet air enter casing 110, first converge in mixing tube 174.By the humidity regulating the first flow control valve 173 on dry air pipe 171 and wet air pipe 172 can control the air passed in mixing tube 174 respectively, and then control to enter the atmospheric moisture passed in casing 110.In the present invention, first flow control valve 173 can be common manual valve, also can be magnetic valve.For the ease of controlling, preferably, first flow control valve 173 is magnetic valve.
Preferably, second control valve 176 can also be provided with on mixing tube 174.The amount of the air entered in casing 110 can be controlled by second control valve 176.
In order to determine the humidity of the air passed in casing 110, preferably, microbe in solid state culture apparatus 100 also comprises the first wet bulb thermometer 151, and this first wet bulb thermometer 151 is arranged on mixing tube 174, and is positioned at vapour pipe 160 upstream.
First wet bulb thermometer 151 can measure the humidity of the air in mixing tube 174, and the humidity value can measured according to the first wet bulb thermometer 151 regulates the first flow control valve 173 on dry air pipe 171 or wet air pipe 172.
For the ease of realizing Automated condtrol, preferably, described Microorganism solid-state culture system can also comprise the first controller, in this case, first flow control valve 173 is preferably magnetic valve, described first controller is electrically connected with the first wet bulb thermometer 151 and first flow control valve 173, to pass through the humid control first flow control valve 173 measured by the first wet bulb thermometer 151.
First can preset a predetermined moisture angle value according to the kind of the microorganism that will cultivate, if the humidity that the first wet bulb thermometer 151 detects is higher or lower than predetermined moisture angle value, described first controller controls the first flow control valve 173 on dry air pipe 171 or the first flow control valve 173 on wet air pipe 172, till the humidity value detected until the first wet bulb thermometer 151 is consistent with predetermined moisture angle value.Preferably, described first controller is PLC.
To cultivate aspergillus niger spore, during cultivation aspergillus niger spore, required atmospheric moisture is 90% to 99%.Therefore, atmospheric moisture 90% to 99% is set as preset value, if send signal to the first controller after the first wet bulb thermometer 151 records humidity value, if the humidity value recorded is not in above-mentioned scope, first controller then controls the first flow control valve 173 on dry air pipe 171 and/or the first flow control valve 173 on wet air pipe 172, till in the scope that the humidity value recorded until the first wet bulb thermometer 151 drops on 90% to 99%.
In some cases, aseptic wet air can be passed into directly in casing 110, in this case, switch-valve 175 can be set on dry air pipe 171.When only needing to pass into aseptic wet air in casing 110, switch-valve 175 can cut out.Until when needing to pass into aseptic dry air and aseptic wet air in casing 110, switch-valve 175 can be opened simultaneously.
In the present invention, first enters the pipe that pipe 120 can be rigidity, also can be flexible pipe, for the ease of installing and saving installing space, preferably, first enter pipe 120 and comprise and first enter first of pipe 120 two ends at this and enter tube stub 121 and be connected to these two first and enter and enter flexible pipe 122 between tube stub 121.Two first one of entering in tube stub 121 are communicated with fluid source (or being responsible for 150), and the lower floor (interval 113) of two first another and the casings 110 entered in tube stub 121 is communicated with.
In order to make material contact with sterile air or water vapour fully, preferably, described microbe in solid state culture apparatus also comprises second and enters pipe 123, and this second one end entering pipe 123 is communicated with the upper strata of casing 110, and the other end is communicated with supervisor 150.Sterile air in supervisor 150 or a water vapour part enter by first the lower floor (interval 113) that pipe 120 enters casing 110, and another part enters by second the upper strata that pipe 123 enters casing 110.
When utilizing described microbe in solid state culture apparatus culturing micro-organisms, need the gas circulation ensured in casing 110, as noted before, vent pipe 130 by the ft connection of the inside of casing 110 with casing 110, thus by the gas discharge in casing 110.As shown in Figure 1 to Figure 3, preferably, one end of vent pipe 130 is fixed on the sidewall of casing 110, the other end extends to the outside of casing 110, the sidewall of vent pipe 130 is provided with multiple through hole, the other end (that is, being positioned at one end of casing 110 outside) is provided with venting port.
Owing to there is pressure difference inside and outside casing 110, therefore, it is inner that the gas in casing 110 enters vent pipe 130 by the through hole on vent pipe 130 sidewall, and it is outside to flow to casing 110 by venting port.
Owing to mostly being the material that wheat bran etc. easily flies away for the substratum of culturing micro-organisms, in order to prevent the through hole on the feed blocking vent pipe 130 that flies away when passing into sterile air or water vapor to material from the lower floor of casing 110, preferably, can in the outside of vent pipe 130 coated screen cloth 131.
For the ease of measuring the moisture absorbed in material, preferably, described microbe in solid state culture apparatus can also comprise discharges extension tube 132, and this discharge extension tube 132 is in the outside of casing 110, and be communicated with vent pipe 130, discharge in extension tube 132 and be provided with the second wet bulb thermometer 133.By contrasting humidity value that the first wet bulb thermometer 151 records and the humidity value that the second wet bulb thermometer 133 records, the moisture that material absorbs can be learnt.
As noted before, the contact area of microorganism and oxygen can be increased by the flowing of gas, ensure the demand to oxygen.And flowing air can also take away carbonic acid gas and the heat of microorganism generation.In order to determine the ventilation in described microbe in solid state culture apparatus, preferably, can also extension tube 132 be above-mentioned arranges under meter 134 in discharge, this under meter 134 is electrically connected with first flow control valve 173 and the first controller (that is, under meter 134 and two first flow control valves 173 are all electrically connected with described first controller).
Setting is with individual predetermined amount of flow value, when the flux values that under meter 34 records is higher or lower than predetermined amount of flow value, first controller controls the first flow control valve 173 on dry air pipe 171 or wet air pipe 172, thus control the flow passing into sterile air in casing 110, until measured value and the predetermined amount of flow of under meter 134 be worth consistent till.
In order to mixing of materials is even, need after material being added into casing 110 inside mixing of materials is even.When mixture, need to rotate casing 110.Therefore, described Microorganism solid-state culture system can also comprise power set 500, and this power set comprises the rotation axis 510 be fixedly connected with the sidewall of casing 110, and this rotation axis 510 can around own axis.Usually, as shown in fig. 1, power set 500 reduction box that also comprises motor and be connected with motor output shaft.Rotation axis 510 is connected with the output shaft of reduction box (can be connected by belt transmission system or chain drive system), rotation axis 510 can be driven to rotate, and then drive casing 110 to rotate during the output shaft rotation of motor.
A large amount of heat can be discharged in the process of microorganism growth, prevent material from luming in order to heat radiation in time and avoid burning song, preferably, in the process of microorganism growth, also can control rotation axis 510 and rotate, thus the heat in material distributing removing in time.The growth of the mycelia of destroy microorganisms when moving with casing 110 to prevent material, the rotational angle of casing 110 is unsuitable excessive.In order to realize this purpose, the second controller that power set 500 can also comprise pulse encoder 520 and be electrically connected with this pulse encoder 520.
In the present invention, the housing of pulse encoder 520 can be fixed on actionless object (such as, the support of support rotating axle 510) on, the code-disc of pulse encoder 520 is then connected with rotation axis 510, the circuit card of pulse encoder 520 is electrically connected with second controller, thus can determine the angle that rotation axis 510 turns over.Certainly, the code-disc of pulse encoder 520 can also be arranged on the output shaft of reduction box, thus according to the angle that the transmitting ratio determination rotation axis 510 between the output shaft of reduction box and rotation axis 510 turns over.The turn signal of the rotation axis 510 detected is passed to second controller by pulse encoder 520, thus controls the angle that turns over of rotation axis 510 by second controller.Be understandable that, second controller is electrically connected with motor, and the angle turned over by the output shaft controlling motor can control the angle that rotation axis 510 turns over.
Described Microorganism solid-state culture system also comprises temperature sensor 141, and this temperature sensor 141 is arranged on the bottom of described casing 110.The temperature of material in the lower floor of casing 110 can be learnt by the temperature value of reading temperature sensor 141, and control the inlet of water vapor or the inlet of sterile air according to this temperature value.
As noted before, heating unit can be provided with in interval 113, therefore, for the ease of the temperature in the control interval 113, preferably, described Microorganism solid-state culture system can also comprise brush 142 and the 3rd control device, brush 142 is arranged on rotation axis 510, and rotation axis 510 can rotate relative to brush 142, temperature sensor 141 is electrically connected with brush 142, and described 3rd controller is electrically connected with described heating unit, described 3rd controller is also electrically connected with temperature sensor 141 simultaneously.
First the predetermined temperature value required for a microorganism growth is set according to microorganism kind, when rotation axis 510 rotates relative to brush 142, brush 142 generation current, and power for temperature sensor 141, temperature sensor 141 detects after the temperature in interval 113 to the 3rd controller transmission of signal.The temperature value that temperature sensor 141 detects by the 3rd controller and predetermined temperature value compare, if the temperature value recorded is lower than predetermined temperature value, then the 3rd controller controls to start described heating unit, if record temperature higher than predetermined temperature value, then the 3rd controller controls to cut out described heating unit.
In the present invention, the first controller, second controller and the 3rd controller can exist simultaneously, also can Individual existence.When the first controller, second controller and the 3rd controller exist simultaneously, Microorganism solid-state culture system of the present invention can also comprise master controller, and described first controller, second controller and the 3rd controller are integrated in master controller.Further, in the present invention, at least one in described first controller, second controller and the 3rd controller can be PLC.
By after the bottom of casing arranges sieve plate, sterile air or water vapor is passed in the bottom casing of body, sterile air or water vapor rise and through the material be deposited on sieve plate, thus make to have less temperature head and psychrometric difference between material the upper and lower.
Below the preferred embodiment of the present invention is described in detail by reference to the accompanying drawings; but; the present invention is not limited to the detail in above-mentioned embodiment; within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each the concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode.In order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible array mode.
In addition, also can carry out arbitrary combination between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (20)
1. a Microorganism solid-state culture system, this Microorganism solid-state culture system comprises microbe in solid state culture apparatus (100) and fluid source (200), fluid in described fluid source (200) comprises sterile air and water vapor, described microbe in solid state culture apparatus comprises casing (110), first enters pipe (120) and vent pipe (130), described first enters pipe (120) is communicated with described fluid source (200), it is characterized in that, described microbe in solid state culture apparatus also comprises the sieve plate (140) with sieve aperture, described sieve plate (140) is arranged in described casing (110), and the internal space of this casing (110) is divided into two-layer up and down, described first enters pipe (120) communicates with the lower floor of described casing (110), described vent pipe (130) is by described casing (110) and ft connection, described sterile air or water vapor rise and through the material be deposited on described sieve plate, thus make, between the upper and lower of material, there is less temperature head and psychrometric difference.
2. Microorganism solid-state culture system according to claim 1, it is characterized in that, described casing comprises box main body (111) and chuck (112), described sieve plate (140) is arranged on the diapire of described box main body (111), the bottom of described box main body (111) is arranged in described chuck (112), and there is interval (113) between the outer wall of described box main body (111) and this chuck (112), described first enters pipe (120) and described interval (113) communicate, the upper end of described chuck (112) and the outside surface of described box main body (111) are tightly connected.
3. Microorganism solid-state culture system according to claim 2, is characterized in that, described microbe in solid state culture apparatus (100) also comprises heating unit, and this heating unit is arranged in described interval (113).
4. Microorganism solid-state culture system according to claim 2, is characterized in that, described sieve plate (140) is formed as tabular.
5. Microorganism solid-state culture system according to claim 1, it is characterized in that, described fluid source (200) comprises vapour source (210) and air source (220), described microbe in solid state culture apparatus (100) comprises supervisor (150), vapour pipe (160) and blast main (170), it is outside that this supervisor (150) is positioned at described casing (110), described first enters pipe (120) is connected with this supervisor (150) in the downstream of described supervisor (150), one end of described vapour pipe (160) is communicated with described vapour source (210), the other end is communicated with described supervisor (150), one end of described blast main (170) is communicated with described air source (220), the other end is communicated with supervisor (150).
6. Microorganism solid-state culture system according to claim 5, it is characterized in that, described Microorganism solid-state culture system also comprises air modifier (300) and air filter (400), the side of described air filter (400) is communicated with described air source, opposite side is communicated with described air modifier (300), described blast main (170) comprises dry air pipe (171), wet air pipe (172) and mixing tube (174), described dry air pipe (171) is communicated with between described air filter (400) and described air modifier (300), described wet air pipe (172) is communicated with in described air modifier (300) downstream, described dry air pipe (171) and described wet air pipe (172) are provided with first flow control valve, described dry air pipe (171) and described wet air pipe (172) meet at described mixing tube (174), this mixing tube (174) is communicated with described supervisor (150).
7. Microorganism solid-state culture system according to claim 6, is characterized in that, described mixing tube (174) is provided with second control valve (176).
8. Microorganism solid-state culture system according to claim 6, it is characterized in that, described microbe in solid state culture apparatus (100) also comprises the first wet bulb thermometer (151), this first wet bulb thermometer (151) is arranged on described mixing tube (174), and is positioned at described vapour pipe (160) upstream.
9. Microorganism solid-state culture system according to claim 8, it is characterized in that, this Microorganism solid-state culture system also comprises the first controller, described first flow control valve is magnetic valve, described first controller is electrically connected with described first wet bulb thermometer (151) and described first flow control valve, to pass through first flow control valve described in the humid control measured by described first wet bulb thermometer (151).
10. Microorganism solid-state culture system according to claim 6, is characterized in that, described dry air pipe (171) is provided with switch-valve (175).
11. Microorganism solid-state culture system according to claim 5, it is characterized in that, described first enters pipe (120) comprises and is positioned at this and first enters first of pipe (120) two ends and enter tube stub and be connected to these two first and enter and enter flexible pipe (122) between tube stub.
12. Microorganism solid-state culture system according to claim 5, it is characterized in that, this microbe in solid state culture apparatus also comprises second and enters pipe (123), this second one end entering pipe (123) is communicated with the upper strata of described casing (110), and the other end is communicated with described supervisor (150).
13. according to the Microorganism solid-state culture system in claim 1 to 12 described in any one, it is characterized in that, one end of described vent pipe (130) is fixed on the sidewall of described casing (110), the other end extends to the outside of described casing (110), the sidewall of described vent pipe (130) is provided with multiple through hole, the described the other end is provided with venting port.
14. Microorganism solid-state culture system according to claim 13, is characterized in that, the outside of described vent pipe (130) is coated with screen cloth (131).
15. Microorganism solid-state culture system according to claim 9, it is characterized in that, one end of described vent pipe (130) is fixed on the sidewall of described casing (110), the other end extends to the outside of described casing (110), the sidewall of described vent pipe (130) is provided with multiple through hole, the described the other end is provided with venting port, this microbe in solid state culture apparatus (100) comprises discharges extension tube (132), this discharge extension tube (132) is in the outside of described casing (110), and be communicated with described vent pipe (130), described discharge extension tube (132) is provided with the second wet bulb thermometer (133).
16. Microorganism solid-state culture system according to claim 15, it is characterized in that, described discharge extension tube (132) is above-mentioned is provided with under meter (134), and described under meter (134) and described first flow control valve are electrically connected with described first controller respectively.
17. according to the Microorganism solid-state culture system in claim 1 to 12 described in any one, it is characterized in that, this Microorganism solid-state culture system also comprises power set (500), this power set comprises the rotation axis (510) be fixedly connected with the sidewall of described casing (110), and this rotation axis (510) can around own axis.
18. Microorganism solid-state culture system according to claim 17, is characterized in that, the second controller that described power set comprises pulse encoder (520) and is electrically connected with this pulse encoder (520).
19. Microorganism solid-state culture system according to claim 17, it is characterized in that, this Microorganism solid-state culture system also comprises temperature sensor (141), and this temperature sensor (141) is arranged on the bottom of described casing (110).
20. Microorganism solid-state culture system according to claim 19, it is characterized in that, this Microorganism solid-state culture system also comprises the brush (142) be arranged on described rotation axis and the 3rd controller be electrically connected with this brush, described rotation axis (510) can rotate relative to described brush (142), described temperature sensor (141) is electrically connected with described brush (142), and described 3rd controller is electrically connected with described heating unit and described temperature sensor (141).
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| CN106723225A (en) * | 2016-12-29 | 2017-05-31 | 尹氏（天津）生物科技有限公司 | A kind of straw feed fermentation tank |
| CN112391270B (en) * | 2020-10-13 | 2023-06-09 | 嘉兴学院 | Rotary solid-state fermentation device with water vapor circulation and application method thereof |
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