M350554 七、指定代表囷： (一） 本案指定代表圖為：第（1 )圖。 (二) 本代表圖之元件符號簡單說明： 第1圖係顯示摘作之賴驗溫_發酵槽之概視圖 侧為冷凝器，為溫度感測器、(3)為删器， 為P里測為、（5)為夾綠溫冷水循環、（6)為控 水出口、⑻為不銹鋼細網、（9)為 ）為控咖 空氣入口的流量。 ⑽為空氣過濾器 '⑼為 八、新型說明： 【新型所屬之技術領域】 上材發酵槽是改良氣舉式發酵槽，將發酵槽的底端加 2堅硬的不_細網和無菌水，此可通人濕空氣，且 方有冷顧，周圍有央層恒溫冷水猶環，故此 可以維持發酵過程中，槽 _過”，_生物生長而產生的發酵:::成：:: 嶋她崎_供和二ί 度的排除，彻輪⑽哪娜細溫度和滋 【先前技術】 長固發酵疋指微生物在低水分含量或低水活性的固態培養基上生 '、、代_發酵過程。將_發酵與液_發酵相比，固態 M350554 酵廢水和廢棄物排放'且耗能低、收成率高，並可 =:乾燥等操作費用。天然或合成的物質都可作為固 I酵的土貝’顆粒狀的穀類如米、小麥、大麥、玉求、大豆與農業 培養的殘餘物，均含有豐富_、_ ϋ 1及木_ «體作輕源_。_發酵工_彻細可用棚態廢棄物處 理、保健用品、醫痒藥。4私曲雄»L· W摩樂口口、生物辰樂、生物燃料、動物飼料藥劑等。 衫響固恶發酵培翻素包括下列幾點： 1.基質的特性：固態基質顆粒的尺寸大小影響到單位體積反應表面 積進而办響菌絲的生長、氧氣的供給、二氧化碳的移出與發酵熱 的移除。過小的顆粒則會造成基質的麵，基質與基質間空隙也變 小，此不利於熱傳與質傳。 2. 基質的水分含量：職發酵基f的水分含量會影響微生物的生長， 典型的基質水分含量約為3〇_8〇%(w/w)，當基質水分含量過高會造 成固態基質堆聚’造成發酵時通氣不良，甚至形成缺氧環境。 3, 溫度：發酵時會產生發酵熱而導致發酵基質溫度升冑，發酵熱的移 除對於熱傳導困難的多孔性固態基質是主要的限制，可搭配間歇性 的噴灑冷卻水。 雖然固發酵技術發展時間甚長，但由於目態發酵參數監測、控制 較困難，基質的翻L較困難’而造成微生物細胞、養分、溫度、水含 量分布不均，較難細於大量的純種培養，使得_猶被隔離於現 代發酵工業之外。_發酵過程巾常會發生基質溫度過高和水分含量 過少的情形’故Hi態發酵錄缝式培養，以利發酵綱的補水、翻 M350554 動降溫’綺不利於_、發_量產，此有賴於_、發酵_設計和 改良，方可使固祕酵能有齡和雜鱗在工料有相同的發展。 固悉發酵設備有大池式、韻式、塔柱式、床式、轉筒式和轉轴式。 【新型内容】 通濕氣恒溫固態發酵槽是改良氣舉式發酵槽，在發酵槽的底端加上 材質堅硬的獨鋼細網’此可防止_歸壓住通氣α，並可在不錢 鋼網下方加人錢水’連接空壓機及空氣磁^，並可調控通入無菌 空氣的流量，此可使通入的空氣濕潤，此濕空氣可進入基質間隙中， 不僅僅是提供飽和水氣触a，也可使二氧魏和熱的移除。且發酵 槽的^方備有HTC的冷卻水，以確保冷凝由射因發_喊發出的 JC、氣而不致於發生因為水分散失而造成基質表層太過乾燥，方可 保持發酵過射基質的Μ。通疏恒溫固態發酵槽並配合夹層恒溫 冷水循環，來㈣發__溫度可將發酵熱移除，避免過多基質造 成發酵熱不錄發而提升發酵溫度。故此通減恒溫_發酵槽可以 、、、寺I酵過私中’基貝的溫度、濕度和氧氣’一併解決固態發酵過程 中’ β因為微生物生長而產生的發酵熱，而造成基f的升溫和水分蒸發 的問題’且此可增加固態基質的深度和氧氣的提供和二氧化碳的排 除’可以省去發酵過程中加水、翻拌而調整基質的溫度和濕度。 【實施方式] 以冬S*夏草於此通濕氣恒溫固態發酵槽進行固態發酵為例： 接種冬蟲夏草預活化菌液於lkg的已殺g冷卻的薏仁基質掉均 後’裝於通職恒溫_發酵槽，於攻下調整通分別為每分鐘 M350554 W 一 8公升進行嶋發酵為例，並於 絲體生成量。當鞠^氣量達 =財刀析制 ，故増加職量村使整翻菌絲快速 不同通氣量對薏仁基質發酵菌 国、糸的表—結果得知，當通氣量為0 LPM發酵7天僅得菌絲量24.46 通孔里巧M350554 VII. Designated representative: (1) The representative representative of the case is: (1). (2) A brief description of the symbol of the representative figure: Figure 1 shows the temperature of the extraction. The overview side of the fermentation tank is the condenser, the temperature sensor, and (3) the eraser. The measurement is (5) for the green warm water circulation, (6) for the water control outlet, (8) for the stainless steel fine mesh, and (9) for the coffee inlet of the coffee control. (10) is the air filter '(9) is eight, the new description: [New technical field] The top fermentation tank is an improved gas lift fermentation tank, adding 2 hard non-fine mesh and sterile water to the bottom end of the fermentation tank. This can pass people's wet air, and there is a cold look, there is a central layer of constant temperature cold water around the ring, so it can maintain the fermentation process, the trough _ over, _ biological growth and fermentation::: into::: 嶋 her Kawasaki _ supply and two degrees of elimination, the full round (10) Nina fine temperature and nourishment [previous technology] long solid fermentation 疋 refers to microorganisms in the low moisture content or low water activity of the solid medium on the ', generation _ fermentation process. Compared with liquid fermentation, solid M350554 fermentation wastewater and waste discharge 'has low energy consumption, high yield, and can be = dry operation costs. Natural or synthetic substances can be used as soil for solidification. Bay's granular cereals such as rice, wheat, barley, jade, soybeans and agricultural culture residues are rich in _, _ ϋ 1 and wood _ «body is light source _. _ fermenter _ carefully available shed Waste treatment, health care products, medical itching medicine. 4 private music male » L · W Morlot , Bio-Chen Le, bio-fuels, animal feed medicines, etc. The following factors are included: 1. The characteristics of the matrix: the size of the solid matrix particles affects the reaction surface area per unit volume and then the hyphae Growth, supply of oxygen, removal of carbon dioxide and removal of heat of fermentation. Too small particles will cause the surface of the matrix, and the space between the matrix and the matrix will also become smaller, which is not conducive to heat transfer and mass transfer. The moisture content of the fermented base f will affect the growth of microorganisms. The typical matrix moisture content is about 3〇_8〇% (w/w). When the moisture content of the matrix is too high, the solid matrix will be piled up to cause fermentation. Poor, even forming an oxygen-deficient environment. 3. Temperature: Fermentation heat is generated during fermentation, which causes the temperature of the fermentation substrate to rise. The removal of fermentation heat is a major limitation for porous solid substrates with difficult heat conduction, and can be combined with intermittent spraying. Cooling water. Although the solid fermentation technology develops for a long time, it is difficult to monitor and control the fermentation parameters of the target, and the matrix L is difficult to make 'microorganisms The distribution of cells, nutrients, temperature and water content is uneven, and it is difficult to be finer than a large number of pure seed cultures, so that it is isolated from the modern fermentation industry. _ Fermentation process often occurs when the substrate temperature is too high and the moisture content is too low. 'So Hi state fermentation recording and cultivating, to facilitate the fermentation of the hydrating, turn M350554 to cool down '绮 is not conducive to _, hair _ mass production, which depends on _, fermentation _ design and improvement, can make solid yeast Ageing and weeds have the same development in the materials. The fermentation equipment has large pool, rhyme, tower, bed, drum and shaft. [New content] The modified gas lift fermentation tank is equipped with a hard steel sole mesh at the bottom end of the fermenter. This prevents _ returning to the ventilation α, and can add money to the bottom of the steel mesh. And air magnetic ^, and can regulate the flow of sterile air, which can humidify the incoming air, this humid air can enter the interstitial space, not only provide saturated water gas contact a, but also dioxetane Hot removal. And the cooling water of the fermenting tank is provided with HTC cooling water to ensure that the condensation is caused by the JC and gas emitted by the injection, so that the surface layer of the substrate is too dry due to the loss of water dispersion, so as to maintain the fermentation matrix. Hey. The temperature-controlled solid-state fermentation tank is combined with the constant temperature cold water circulation of the interlayer, and the heat of the fermentation can be removed by the __ temperature, so as to avoid the excessive heat of the substrate and the fermentation temperature. Therefore, the constant temperature _ fermenting tank can,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, The problem of temperature rise and water evaporation 'and this can increase the depth of the solid matrix and the supply of oxygen and the elimination of carbon dioxide' can eliminate the temperature and humidity of the substrate by adding water and mixing during the fermentation process. [Embodiment] Taking S* Xia Cao in this humidified thermostatic solid-state fermentation tank for solid-state fermentation as an example: Inoculation of the Cordyceps sinensis pre-activated bacterial liquid in the lkg of the g-cooled coix seed matrix after the average is 'installed in the constant temperature _ Fermentation tank, the adjustment of the pass is M350554 W per minute, 8 liters per minute for the fermentation of sputum, and the amount of silk production. When the amount of gas is up to the value of the knife, so the village has increased the amount of ventilation of the whole mycelium to the table of the fermenting bacteria of the coix seed, and the results show that when the aeration is 0 LPM fermentation for 7 days only The amount of hyphae is 24.46
Sg且菌絲僅生長在基質上層，需發 酵第14天_體方可長滿整罐:射，所得到的菌絲含量約為猶2 mg/g ’通氣量為2 LPM、4 LPM和6 LPM發酵7天後的菌絲含量分別為 31.04 mg/g、32.72 mg/g及107.49 mg/g ’當通氣量達到8 LpM僅需發酵心 天即可獲得115.56 mg/g ’隨著通入濕空氣量，可顯著增加菌絲生成量， 並大幅縮短量產製程的發酵時間。 表一、不同通氣量對薏仁基質發酵菌絲的影響 濕空氣流量（L/min) 發酵時間（day) 菌絲量（mg/g) 0 7 24.46士 0.44 2 7 31.04±0.19 4 7 32.72土 0.48 6 7 107.49±〇-〇3 8 6 115.56 士 0.43 M350554 【圖式簡單說明】 態發酵槽之概視圖。 第1圖係顯示本_之魏氣,陵溫固 【主要元件符號說明】 第1圖中的代號(1)為冷凝写 .^ 〇 (2)為溫度感測器、（3)為濕度偵測器、（4) 為pH監測器、（5)為夾層择 ，義令水循環、（6)為控溫水入口、（7)為押㈤ 水出口、⑻為不銹鋼細網 Μ控/皿 空氣入口的流量。 ）為曝氣頭、⑽為空氣過濾、器、⑴)為 九、申請專利範圍： 1. 一種通濕氣恒溫固態發赌抽 口用以投入讎和陶;;發酵槽設有掀式上蓋和進料口，進料 溫固態發酵觀配合絲酵獅均财冷翻，通濕氣恒 Μ⑽“ + 恤冷水循環；發酵槽的底端加上材質堅硬 的不銹鋼細網，在不銹鋼锢 4貝父 謂卿方加入無菌水，並調控 透過底部曝氣頭，以通入濕空氣。 、八工巩的抓里 2. 如申請專利範圍第1項 ΠΛ、衫⑴、職、發酵槽，其中掀蓋頂部上 方汉有冷如⑴，亚有_ D可裝設溫度感測 】 和pH監測器(4)。 （、、、度偵測為(3) 3. 如申明專利⑱圍弟1項之通濕氣恒溫固態發酵槽，發酵 冷水循環⑶糾___溫度，⑹為控溫权Λ日日•一观 4·如申請W_ 1歡顧紐溫_發_，_水出 銹鋼細網⑻以防止_基純住曝氣口，如㈣沒有 ^卜罈綱細網的下方加入Sg and hyphae grow only in the upper layer of the substrate, and it takes 14 days to ferment _ body can grow up to the whole tank: shot, the obtained hyphae content is about 2 mg / g 'ventilation is 2 LPM, 4 LPM and 6 The mycelial content after 7 days of LPM fermentation was 31.04 mg/g, 32.72 mg/g and 107.49 mg/g, respectively. When the aeration reached 8 LpM, only 115.56 mg/g of fermented heart was obtained. The amount of air can significantly increase the amount of mycelial production and significantly shorten the fermentation time of the mass production process. Table 1. Effect of different aeration on the fermentation of mycelium of coix seed. Wet air flow (L/min) Fermentation time (day) Mycelial amount (mg/g) 0 7 24.46 ± 0.44 2 7 31.04 ± 0.19 4 7 32.72 0.48 6 7 107.49±〇-〇3 8 6 115.56 ± 0.43 M350554 [Simple description of the diagram] Overview of the fermentation tank. The first picture shows the Wei gas of this _, Ling Wengu [main component symbol description] The code (1) in the first figure is written for condensation. ^ 〇 (2) is the temperature sensor, (3) is the humidity detector Detector, (4) is the pH monitor, (5) is the interlayer selection, the water is ordered, (6) is the temperature control water inlet, (7) is the (5) water outlet, and (8) is the stainless steel fine mesh Μ control / dish air The flow of the entrance. ) is the aeration head, (10) is the air filter, the device, (1)) is nine, the scope of the patent application: 1. a constant temperature solid-state gambling mouth is used to put the pottery and pottery; the fermentation tank is provided with a squat top cover and Feeding port, feed temperature, solid state fermentation view with silky lions, all cold, turn moisture, constant moisture (10) "+ shirt cold water circulation; the bottom of the fermentation tank is made of hard stainless steel fine mesh, in stainless steel 锢 4 shellfish It is said that Qingfang adds sterile water and regulates the passage of the aeration head through the bottom to pass the humid air. The grasp of the eight Gonggong 2. If the patent application scope is the first item, the shirt (1), the job, the fermentation tank, and the cover At the top of the top, there are cold as (1), sub- _ D can be installed with temperature sensing] and pH monitor (4). (,, and degree detection is (3) 3. If the patent is 18 Moisture-temperature solid-state fermentation tank, fermentation cold water circulation (3) Correction ___temperature, (6) Temperature control right Λ Day • One view 4 · If applying W_ 1 Welcome to New York _ hair _, _ water rust steel fine mesh (8) In order to prevent _ base from ablation of the aeration port, such as (4) did not join the bottom of the fine mesh