CN103695294A - Microorganism solid-state culture system - Google Patents
Microorganism solid-state culture system Download PDFInfo
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- CN103695294A CN103695294A CN201310718455.2A CN201310718455A CN103695294A CN 103695294 A CN103695294 A CN 103695294A CN 201310718455 A CN201310718455 A CN 201310718455A CN 103695294 A CN103695294 A CN 103695294A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses a microorganism solid-state culture system. The microorganism solid-state culture system comprises a microorganism solid-state culture device (100) and a fluid source (200), wherein the microorganism solid-state culture device comprises a box body (110), a first inlet pipe (120) and a discharge pipe (130); the first inlet pipe (120) is communicated with the fluid source (200); the microorganism solid-state culture device further comprises a sieve plate (140) with sieve meshes; the sieve plate (140) is arranged in the box body (110) and divides the internal space of the box body (110) into upper and lower layers; the first inlet pipe (120) is communicated with the lower layer of the box body (110); the box body (110) is communicated with the outside via the discharge pipe (130). Since the sieve plate is arranged at the bottom of the box body, sterile air or water vapor is introduced from the box body at the bottom of the system and rises up and passes through materials stacked on the sieve plate, so that temperature difference and humidity difference between the upper and lower layers of the materials are relatively small.
Description
Technical field
The present invention relates to the culture device of microorganism, particularly, relate to a kind of microbe in solid state culture systems.
Background technology
Solid state fermentation is one of human use's microorganisms producing product technology with the longest history, and the time-honored brewing industry of China is the Typical Representative of solid state fermentation.Solid state fermentation contains water-fast solid, a small amount of moisture and air, the thermal conductance that microorganism generates causes moisture evaporation, make fermentation system there is the solid inhomogeneous three-phase of vapour-liquid, there is serious concentration gradient and heat transfer, mass transfer difficulty, be difficult to like this control pH, water activity, optimal reaction temperature etc., output is declined greatly, the most important condition of especially modern fermentation technique is purebred cultivation, do not allow natural other microorganism to enter, cause living contaminants, add the requirement of modern industry to extensive intensive production, the production application of solid state fermentation is stayed cool, almost be ostracised outside modern industry.Yet in recent years, day by day serious due to energy dilemma and environmental problem, solid-state fermentation technology causes people's interest again, there is earth-shaking variation in the research in solid state fermentation field.
In CN102212472A, disclose a kind of automatic rotary microbial spore incubator, this automatic rotary microbial spore incubator mainly comprises that incubator, sterile air add pipe and steam to add pipe.When culturing micro-organisms, material is added in incubator, then by sterile air heating tube and steam, add pipe to pass into sterile air and steam in incubator.But in this automatic rotary microbial spore incubator, the bottom of solid accumulation incubator, makes the gradient difference of material bed upper and lower temperature and humidity larger, is unfavorable for the growth of microbial spore.
Therefore the gradient difference that, how to reduce temperature and humidity up and down material bed in microbe in solid state culture apparatus becomes this area technical problem urgently to be resolved hurrily.
Summary of the invention
The object of this invention is to provide a kind of microbe in solid state culture systems, material bed upper and lower temperature and humidity in this microbe in solid state culture systems are more even.
To achieve these goals, the invention provides a kind of microbe in solid state culture systems, this microbe in solid state culture systems comprises microbe in solid state culture apparatus and fluid source, this microbe in solid state culture apparatus comprises casing, first enters pipe and vent pipe, described first enters pipe is communicated with described fluid source, wherein, described microbe in solid state culture apparatus also comprises the sieve plate with sieve aperture, described sieve plate is arranged in described casing, and the internal space of this casing is divided into two-layer up and down, described first enters pipe communicates with the lower floor of described casing, described vent pipe is communicated with described casing with outside.
Preferably, described casing comprises box main body and chuck, described sieve plate is arranged on the diapire of described box main body, the bottom of described box main body is arranged in described chuck, and there is interval between the outer wall of described box main body and this chuck, described first enters pipe communicates with described interval, and the upper end of described chuck and the outside surface of described box main body are tightly connected.
Preferably, described microbe in solid state culture apparatus also comprises heating unit, and this heating unit is arranged in described interval.
Preferably, described sieve plate forms tabular.
Preferably, described fluid source comprises vapour source and air source, described microbe in solid state culture apparatus comprises supervisor, vapour pipe and blast main, it is outside that this supervisor is positioned at described casing, described first enters pipe is connected with this supervisor in described supervisor's downstream, and one end of described vapour pipe is communicated with described vapour source, and the other end is communicated with described supervisor, one end of described blast main is communicated with described air source, and the other end is communicated with supervisor.
Preferably, described microbe in solid state culture systems also comprises air modifier and air filter, one side of described air filter is communicated with described air source, opposite side is communicated with described air modifier, described blast main comprises dry air pipe, wet air pipe pipe and mixing tube, described dry air pipe is communicated with between described air filter and described air modifier, described wet air pipe is communicated with in described air modifier downstream, on described dry air pipe and described wet air pipe, be provided with first flow control valve, described dry air pipe and described wet air pipe meet at described mixing tube, this mixing tube is communicated with described supervisor.
Preferably, on described mixing tube, be provided with second control valve.
Preferably, described microbe in solid state culture apparatus also comprises the first wet bulb thermometer, and this first wet bulb thermometer is arranged on described mixing tube, and is positioned at described vapour pipe upstream.
Preferably, this microbe in solid state culture systems also comprises the first controller, described first flow control valve is magnetic valve, described the first controller is electrically connected to described the first wet bulb thermometer and described first flow control valve, to control described first flow control valve by the measured humidity of described the first wet bulb thermometer.
Preferably, on described dry air pipe, be provided with switch-valve.
Preferably, described first enter pipe and comprise at this and first enter first entering tube stub and be connected to this two first flexible pipes that enter that enter between tube stub of pipe two ends.
Preferably, this microbe in solid state culture apparatus also comprises that second enters pipe, and this second enters one end of pipe and the upper strata of described casing is communicated with, and the other end is communicated with described supervisor.
Preferably, one end of described vent pipe is fixed on the sidewall of described casing, and the other end extends to the outside of described casing, is provided with a plurality of through holes on the sidewall of described vent pipe, on the described the other end, is provided with venting port.
Preferably, the outside of described vent pipe is coated with screen cloth.
Preferably, described microbe in solid state culture apparatus comprises discharge extension tube, and this discharges extension tube in the outside of described casing, and is communicated with described vent pipe, in described discharge extension tube, is provided with the second wet bulb thermometer.
Preferably, the above-mentioned under meter that is provided with of described discharge extension tube, this under meter is electrically connected to described first flow control valve and described the first controller.
Preferably, described microbe in solid state culture systems also comprises power set, and this power set comprises the rotation axis being fixedly connected with the sidewall of described casing, and this rotation axis can rotate around self axis.
Preferably, described power set comprises pulse encoder and the second controller being electrically connected to this pulse encoder.
Preferably, also comprise temperature sensor, this temperature sensor is arranged on the bottom of described casing.
Preferably, described microbe in solid state culture systems also comprises the brush being arranged on described rotation axis and the 3rd controller being electrically connected to this brush, described rotation axis can rotate relative to described brush, described temperature sensor is electrically connected to described brush, and described the 3rd controller is electrically connected to described heating unit
By the bottom at casing, arrange after sieve plate, in the bottom casing of body, pass into sterile air or water vapor, sterile air or water vapor rise and pass the material being deposited on sieve plate, thereby make between material the upper and lower, to have less temperature head and psychrometric difference.
Other features and advantages of the present invention partly in detail are described the embodiment subsequently.
Accompanying drawing explanation
Accompanying drawing is to be used to provide a further understanding of the present invention, and forms a part for specification sheets, is used from explanation the present invention, but is not construed as limiting the invention with embodiment one below.In the accompanying drawings:
Fig. 1 is the schematic diagram of microbe in solid state culture systems provided by the invention;
Fig. 2 is the schematic diagram of the microbe in solid state culture apparatus in the microbe in solid state culture systems shown in Fig. 1;
With Fig. 3 be the vertical view of the preferred implementation of microbe in solid state culture systems provided by the present invention.
Description of reference numerals
100 microbe in solid state culture apparatus 110 casings
111 box main body 112 chucks
113 intervals 120 first enter pipe
121 first enter tube stub 122 enters flexible pipe
123 second enter pipe 130 vent pipes
131 screen clothes 132 are discharged extension tube
133 second wet bulb thermometer 134 under meters
140 sieve plate 141 temperature sensors
142 brush 150 supervisors
151 first wet bulb thermometer 160 vapour pipes
170 blast main 171 dry air pipes
172 wet air pipe 173 first flow control valves
174 mixing tube 175 switch-valves
200 fluid source 210 vapour sources
220 air source 300 air modifiers
400 air filter 500 power set
510 rotation axis 520 pulse encoders
176 second control valves
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is elaborated.Should be understood that, embodiment described herein only, for description and interpretation the present invention, is not limited to the present invention.
As shown in Figure 1 to Figure 3, the invention provides a kind of microbe in solid state culture systems, this microbe in solid state culture systems comprises microbe in solid state culture apparatus 100 and fluid source 200, this microbe in solid state culture apparatus comprises casing 110, first enters pipe 120 and vent pipe 130, first enters pipe 120 is communicated with fluid source 200, wherein, microbe in solid state culture apparatus also comprises the sieve plate 140 with sieve aperture, sieve plate 140 is arranged in casing 110, and the internal space of this casing 110 is divided into two-layer up and down, first enters pipe 120 communicates with the lower floor of casing 110, vent pipe 130 is communicated with casing 110 with outside.
Fluid in fluid source 200 comprises sterile air and water vapor, water vapor or sterile air can enter the lower floor that pipe 120 enters casing 110 by first, then the sieve aperture by sieve plate 140 enters in material, along with water vapor or sterile air continue pass into, water vapor or sterile air arrive the upper strata of casing 110 from the bottom of material through material, thereby all materials can be contacted fully with water vapor or sterile air, reduced temperature head and the psychrometric difference of material upper strata and material lower floor.
When water vapor or sterile air are through sieve plate 140 and material, entering can be by vent pipe 130 from the interior discharge of casing 110 behind the upper strata of casing 110.
In the present invention, the inside that sieve plate 140 can be set directly at casing 110 is divided into the internal space of casing 110 two-layer up and down.Preferably, casing 110 comprises box main body 111 and chuck 112, sieve plate 140 is arranged on the diapire of box main body 111, the bottom of box main body 111 is arranged in chuck 112, and there is interval 113 between the outer wall of box main body 111 and this chuck 112, first enters pipe 120 communicates with interval 113, and the outside surface of the upper end of chuck 112 and box main body 111 is tightly connected.Should be understood that, " interval 113 " described herein is above described " lower floor of casing 110 ".
Can enter pipe 120 water vapors that fluid source 200 is provided or sterile air by first passes in interval 113.Water vapor or sterile air enter in material by the sieve aperture of sieve plate 140.The advantage that chuck 112 is set is, after water vapor or sterile air are passed in chuck 112, is conducive to box main body 111 to be incubated, thereby is conducive to the microbial growth in box main body 111.
The advantage that the outside surface of the upper end of chuck 112 and box main body 111 is tightly connected is, the sterile air or the water vapour that pass in chuck 112 can not flow to the outside of casing 110, and outside air also can not enter the inside of chuck 112.
In the present invention, can utilize metallic substance (for example, steel) to make chuck 112, and chuck 112 can be made into spherical shell shape, cover on the outside of the bottom of box main body 111.Can utilize the mode of welding that chuck 112 is fixed on box main body 111.
In the present invention, sieve plate 40 can form the diapire of box main body 11,, processes a plurality of sieve apertures on the diapire of box main body 11 that is, thereby makes the diapire of box main body 11 form sieve plate 40.In addition, can also on the diapire of box main body 11, process a through hole, then the sieve plate 40 with sieve aperture is arranged in described through hole.
For the homo(io)thermism in guard box main body 111, preferably, microbe in solid state culture apparatus 100 can also comprise heating unit, and this heating unit is arranged in described interval 113.Described heating unit can be electric heater unit, can be also other forms of heating unit.When the interior temperature in interval 113 is too low, can start described heating unit, when the 113 interior excess Temperature of interval, can stop described heating unit.
Preferably, sieve plate 140 forms tabular.Sieve plate 140 is set to tabular and is not only convenient to processing and manufacturing, but also can on flat sieve plate 140, place a large amount of materials.
As mentioned above, when culturing micro-organisms, need in microbial cultivation device, pass into water vapor and sterile air.The major function that passes into water vapor is to guarantee to have sufficient moisture and high-temperature sterilization in material (being mainly substratum), the major function that passes into sterile air is to guarantee the needed oxygen of microorganism growth, and takes away heat and the carbonic acid gas producing in microorganism growth process.Can utilize same pipe first backward first to enter pipe 20 and pass into sterile air and water vapor.
For the ease of operation, preferably, fluid source 200 comprises vapour source 210 and air source 220, microbe in solid state culture apparatus 100 comprises supervisor 150, vapour pipe 160 and blast main 170, and this supervisor 150 is positioned at casing 110 outsides, and first enters pipe 120 is connected with this supervisor 150 in supervisor 150 downstream, one end of vapour pipe 160 is communicated with vapour source 210, the other end is communicated with supervisor 150, and one end of blast main 170 is communicated with air source 220, and the other end is communicated with supervisor 150.The water vapor that vapour source 210 provides enters in supervisor 150 by vapour pipe 160, and the air that air source 220 provides enters in supervisor 150 by blast main 170.Water vapor or air in supervisor 150 are passed in interval 113.
One skilled in the art will appreciate that when culturing micro-organisms, not in casing 110, pass into water vapor and sterile air simultaneously.But first in casing 110, pass into water vapor, thus carry out High Temperature Sterilization, then in casing 110, pass into sterile air.
The important factor when humidity in casing 110 is culturing micro-organisms.The interior humidity of casing 110 is too small, and the moisture in material easily evaporates, thereby material is become dry and affect microbial growth; If the humidity in casing 110 is excessive, can affect the oxygen level in casing 110, cause environmental hypoxia, but also can become wet because moisture in air condensation makes material surface, affect microbial growth or contaminating microorganisms.
In order to obtain sterile air, first to filter air, obtain aseptic dry air, in order to obtain aseptic wet air, also to carry out humidification to aseptic dry air.Therefore, described microbe in solid state culture systems also comprises air modifier 300 and air filter 400, and a side of air filter 400 is communicated with air source 220, and opposite side is communicated with air modifier 300.
In order to guarantee the humidity of the material in casing 110, need in casing 110, pass into aseptic wet air.The air that air source 220 provides obtains aseptic dry air after air filter 400, and aseptic dry air is passed in air modifier 300 and can obtain aseptic wet air.Can control the atmospheric moisture in casing 110 by controlling the humidity of aseptic wet air.
For the ease of controlling the atmospheric moisture in casing 110, preferably, blast main 170 can comprise dry air pipe 171, wet air pipe 172 pipes, a part for the sterile air that the empty vapour that air source 220 provides obtains through air filter 400 directly enters in casing 110 by dry air pipe 171, another part is introduced into air modifier 300 to carry out humidification and obtains aseptic wet air, and then enter wet air pipe 172, and enter in casing 110 by wet air pipe 172.
In order to be further convenient to control the atmospheric moisture in casing 10, blast main 170 can also comprise mixing tube 174, dry air pipe 171 is communicated with between air filter 400 and air modifier 300, wet air pipe 172 is communicated with in air modifier 300 downstreams, on dry air pipe 171 and wet air pipe 172, be provided with first flow control valve 173, dry air pipe 171 and wet air pipe 172 meet at mixing tube 174, and this mixing tube 174 is communicated with supervisor 150.
Before aseptic dry air and aseptic wet air enter casing 110, first in mixing tube 174, converge.By regulating respectively the first flow control valve 173 on dry air pipe 171 and wet air pipe 172 can control the humidity that passes into the air in mixing tube 174, and then control enters the atmospheric moisture passing in casing 110.In the present invention, first flow control valve 173 can be common manual valve, can be also magnetic valve.For the ease of controlling, preferably, first flow control valve 173 is magnetic valve.
Preferably, can also on mixing tube 174, be provided with second control valve 176.By second control valve 176, can control the amount that enters the air in casing 110.
In order to determine the humidity that passes into the air in casing 110, preferably, microbe in solid state culture apparatus 100 also comprises the first wet bulb thermometer 151, and this first wet bulb thermometer 151 is arranged on mixing tube 174, and is positioned at vapour pipe 160 upstreams.
The first wet bulb thermometer 151 can be measured the humidity of the air in mixing tube 174, and the humidity value that can measure according to the first wet bulb thermometer 151 regulates the first flow control valve 173 on dry air pipe 171 or wet air pipe 172.
For the ease of realizing automatization, control, preferably, described microbe in solid state culture systems can also comprise the first controller, in this case, first flow control valve 173 is preferably magnetic valve, described the first controller is electrically connected to the first wet bulb thermometer 151 and first flow control valve 173, to control first flow control valve 173 by the measured humidity of the first wet bulb thermometer 151.
Can first according to the kind of the microorganism that will cultivate, preset a predetermined moisture value, if the humidity that the first wet bulb thermometer 151 detects is higher or lower than predetermined moisture value, first flow control valve 173 on described the first controller control dry air pipe 171 or the first flow control valve 173 on wet air pipe 172, until the humidity value that the first wet bulb thermometer 151 detects is consistent with predetermined moisture value.Preferably, described the first controller is PLC controller.
The cultivation aspergillus niger spore of take is example, and while cultivating aspergillus niger spore, required atmospheric moisture is 90% to 99%.Therefore, atmospheric moisture 90% is set as to preset value to 99%, if the first wet bulb thermometer 151 records humidity value, backward the first controller sends signal, if the humidity value recording is not in above-mentioned scope, the first controller is controlled first flow control valve 173 on dry air pipe 171 and/or the first flow control valve 173 on wet air pipe 172, until the humidity value that the first wet bulb thermometer 151 records drops in 90% to 99% scope.
In some cases, can directly in casing 110, pass into aseptic wet air, in this case, switch-valve 175 can be set on dry air pipe 171.Only need to when passing into aseptic wet air in casing 110, switch-valve 175 can cut out.Until while needing simultaneously to pass into aseptic dry air and aseptic wet air in casing 110, switch-valve 175 can be opened.
In the present invention, first to enter pipe 120 can be the pipe of rigidity, also can be flexible pipe, for the ease of installing and save installing space, preferably, first enter pipe 120 and comprise at this and first enter first of pipe 120 two ends and enter tube stub 121 and be connected to these two first and enter and enter flexible pipe 122 between tube stub 121.Two first enter in tube stub 121 one and are communicated with fluid source (or supervisor 150), and two first enter another in tube stub 121 and are communicated with the lower floor (interval 113) of casing 110.
For material is contacted with sterile air or water vapour fully, preferably, described microbe in solid state culture apparatus also comprises that second enters pipe 123, and this second one end that enters pipe 123 is communicated with the upper strata of casing 110, and the other end is communicated with supervisor 150.Sterile air in supervisor 150 or a water vapour part enter by first the lower floor (interval 113) that pipe 120 enters casing 110, and another part enters by second the upper strata that pipe 123 enters casing 110.
When utilizing described microbe in solid state culture apparatus culturing micro-organisms, need to guarantee the gas communication in casing 110, as noted before, vent pipe 130 is communicated with the inside of casing 110 with the outside of casing 110, thereby the gas in casing 110 is discharged.As shown in Figure 1 to Figure 3, preferably, one end of vent pipe 130 is fixed on the sidewall of casing 110, the other end extends to the outside of casing 110, on the sidewall of vent pipe 130, be provided with a plurality of through holes, on the other end (that is, being positioned at one end of casing 110 outsides), be provided with venting port.
Owing to there is pressure difference inside and outside casing 110, therefore, the gas in casing 110 enters vent pipe 130 inside by the through hole on vent pipe 130 sidewalls, and flows to casing 110 outsides by venting port.
Because the substratum for culturing micro-organisms mostly is the material that wheat bran etc. easily flies away, through hole the feed blocking vent pipe 130 flying away in order to prevent from passing into sterile air or water vapor in from the lower floor from casing 110 to material, preferably, can be coated screen cloth 131 in the outside of vent pipe 130.
For the ease of measuring the moisture absorbing in material, preferably, described microbe in solid state culture apparatus can also comprise discharges extension tube 132, and this discharges extension tube 132 in the outside of casing 110, and be communicated with vent pipe 130, discharge in extension tube 132 and be provided with the second wet bulb thermometer 133.The humidity value recording by contrasting humidity value that the first wet bulb thermometer 151 records and the second wet bulb thermometer 133, can learn the moisture that material absorbs.
As noted before, the mobile contact area that can increase microorganism and oxygen by gas, guarantees the demand to oxygen.And flowing air can also be taken away carbonic acid gas and the heat of microorganisms.In order to determine the ventilation in described microbe in solid state culture apparatus, preferably, the above-mentioned under meter 134 that arranges of extension tube 132 can also discharged, this under meter 134 is electrically connected to (that is, under meter 134 and two first flow control valves 173 are all electrically connected to described the first controller) with first flow control valve 173 and the first controller.
Setting is with individual predetermined amount of flow value, the flux values recording when under meter 34 is during higher or lower than predetermined amount of flow value, the first controller is controlled the first flow control valve 173 on dry air pipe 171 or wet air pipe 172, thereby control the flow that passes into the sterile air in casing 110, until the measured value of under meter 134 is consistent with predetermined amount of flow value.
For mixing of materials is even, need to be after material being added into casing 110 inside, mixing of materials is even.When mixture, need to rotate casing 110.Therefore, described microbe in solid state culture systems can also comprise power set 500, and this power set comprises the rotation axis 510 being fixedly connected with the sidewall of casing 110, and this rotation axis 510 can rotate around self axis.Conventionally, as shown in fig. 1, power set 500 also comprises motor and the reduction box being connected with motor output shaft.Rotation axis 510 is connected with the output shaft of reduction box (can be connected by belt transmission system or chain drive system), can drive rotation axis 510 to rotate, and then drive casing 110 to rotate during the output shaft rotation of motor.
In the process of microorganism growth, can discharge a large amount of heat, for heat radiation in time prevents material, lump and avoid burning bent, preferably, in the process of microorganism growth, also can control rotation axis 510 and rotate, thereby the heat in material distribute and removes in time.The growth of the mycelia of destroy microorganisms when preventing material with casing 110 motion, the rotational angle of casing 110 is unsuitable excessive.In order to realize this purpose, power set 500 can also comprise pulse encoder 520 and the second controller being electrically connected to this pulse encoder 520.
In the present invention, the housing of pulse encoder 520 (for example can be fixed on actionless object, the support of support rotating axle 510) on, the code-disc of pulse encoder 520 is connected with rotation axis 510, the circuit card of pulse encoder 520 is electrically connected to second controller, thereby can determine the angle that rotation axis 510 turns over.Certainly, the code-disc of pulse encoder 520 can also be arranged on the output shaft of reduction box, thereby determine according to the transmitting ratio between the output shaft of reduction box and rotation axis 510 angle that rotation axis 510 turns over.Pulse encoder 520 passes to second controller by the turn signal of the rotation axis detecting 510, thereby controls by second controller the angle that rotation axis 510 turns over.Be understandable that, second controller is electrically connected to motor, by controlling the angle that the output shaft of motor turns over, can control the angle that rotation axis 510 turns over.
Described microbe in solid state culture systems also comprises temperature sensor 141, and this temperature sensor 141 is arranged on the bottom of described casing 110.By the temperature value of reading temperature sensor 141, can learn the temperature of material in the lower floor of casing 110, and control the inlet of water vapor or the inlet of sterile air according to this temperature value.
As noted before, in interval 113, can be provided with heating unit, therefore, for the ease of the temperature in the control interval 113, preferably, described microbe in solid state culture systems can also comprise brush 142 and the 3rd control device, brush 142 is arranged on rotation axis 510, and rotation axis 510 can rotate with respect to brush 142, temperature sensor 141 is electrically connected to brush 142, and described the 3rd controller is electrically connected to described heating unit, described the 3rd controller is also electrically connected to temperature sensor 141 simultaneously.
First according to microorganism kind, set a needed predetermined temperature value of microorganism growth, when rotation axis 510 rotates with respect to brush 142, brush 142 generation currents, and be temperature sensor 141 power supplies, temperature sensor 141 detects backward the 3rd controller transmission of signal of temperature in interval 113.Temperature value and predetermined temperature value that the 3rd controller detects temperature sensor 141 compare, if the temperature value recording is lower than predetermined temperature value, the 3rd controller is controlled and is started described heating unit, if record temperature higher than predetermined temperature value, the 3rd controller controls and cuts out described heating unit.
In the present invention, the first controller, second controller and the 3rd controller can exist simultaneously, also can Individual existence.When the first controller, second controller and the 3rd controller exist simultaneously, microbe in solid state culture systems of the present invention can also comprise master controller, and described the first controller, second controller and the 3rd controller are integrated in master controller.And in the present invention, at least one in described the first controller, second controller and the 3rd controller can be PLC controller.
By the bottom at casing, arrange after sieve plate, in the bottom casing of body, pass into sterile air or water vapor, sterile air or water vapor rise and pass the material being deposited on sieve plate, thereby make between material the upper and lower, to have less temperature head and psychrometric difference.
Below describe by reference to the accompanying drawings the preferred embodiment of the present invention in detail; but; the present invention is not limited to the detail in above-mentioned embodiment; within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition each the concrete technical characterictic described in above-mentioned embodiment, in reconcilable situation, can combine by any suitable mode.For fear of unnecessary repetition, the present invention is to the explanation no longer separately of various possible array modes.
In addition, between various embodiment of the present invention, also can carry out arbitrary combination, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (20)
1. a microbe in solid state culture systems, this microbe in solid state culture systems comprises microbe in solid state culture apparatus (100) and fluid source (200), this microbe in solid state culture apparatus comprises casing (110), first enters pipe (120) and vent pipe (130), described first enters pipe (120) is communicated with described fluid source (200), it is characterized in that, described microbe in solid state culture apparatus also comprises the sieve plate (140) with sieve aperture, described sieve plate (140) is arranged in described casing (110), and the internal space of this casing (110) is divided into two-layer up and down, described first enters pipe (120) communicates with the lower floor of described casing (110), described vent pipe (130) is communicated with described casing (110) with outside.
2. microbe in solid state culture systems according to claim 1, it is characterized in that, described casing comprises box main body (111) and chuck (112), described sieve plate (140) is arranged on the diapire of described box main body (111), the bottom of described box main body (111) is arranged in described chuck (112), and there is interval (113) between the outer wall of described box main body (111) and this chuck (112), described first enters pipe (120) communicates with described interval (113), the outside surface of the upper end of described chuck (112) and described box main body (111) is tightly connected.
3. microbe in solid state culture systems according to claim 2, is characterized in that, described microbe in solid state culture apparatus (100) also comprises heating unit, and this heating unit is arranged in described interval (113).
4. microbe in solid state culture systems according to claim 2, is characterized in that, described sieve plate (140) forms tabular.
5. microbe in solid state culture systems according to claim 1, it is characterized in that, described fluid source (200) comprises vapour source (210) and air source (220), described microbe in solid state culture apparatus (100) comprises supervisor (150), vapour pipe (160) and blast main (170), this supervisor (150) is positioned at described casing (110) outside, described first enters pipe (120) is connected with this supervisor (150) in described supervisor's (150) downstream, one end of described vapour pipe (160) is communicated with described vapour source (210), the other end is communicated with described supervisor (150), one end of described blast main (170) is communicated with described air source (220), the other end is communicated with supervisor (150).
6. microbe in solid state culture systems according to claim 5, it is characterized in that, described microbe in solid state culture systems also comprises air modifier (300) and air filter (400), one side of described air filter (400) is communicated with described air source, opposite side is communicated with described air modifier (300), described blast main (170) comprises dry air pipe (171), wet air pipe (172) pipe and mixing tube (174), described dry air pipe (171) is communicated with between described air filter (400) and described air modifier (300), described wet air pipe (172) is communicated with in described air modifier (300) downstream, on described dry air pipe (171) and described wet air pipe (172), be provided with first flow control valve (173), described dry air pipe (171) and described wet air pipe (172) meet at described mixing tube (174), this mixing tube (174) is communicated with described supervisor (150).
7. microbe in solid state culture systems according to claim 6, is characterized in that, is provided with second control valve (176) on described mixing tube (174).
8. microbe in solid state culture systems according to claim 6, it is characterized in that, described microbe in solid state culture apparatus (100) also comprises the first wet bulb thermometer (151), and it is upper that this first wet bulb thermometer (151) is arranged on described mixing tube (174), and be positioned at described vapour pipe (160) upstream.
9. microbe in solid state culture systems according to claim 8, it is characterized in that, this microbe in solid state culture systems also comprises the first controller, described first flow control valve (173) is magnetic valve, described the first controller is electrically connected to described the first wet bulb thermometer (151) and described first flow control valve (173), to control described first flow control valve (173) by the measured humidity of described the first wet bulb thermometer (151).
10. microbe in solid state culture systems according to claim 6, is characterized in that, is provided with switch-valve (175) on described dry air pipe (171).
11. microbe in solid state culture systems according to claim 5, it is characterized in that, described first enters pipe (120) comprises that being positioned at this first enters first of pipe (120) two ends and enter tube stub (121) and be connected to these two first and enter and enter flexible pipe (122) between tube stub (121).
12. microbe in solid state culture systems according to claim 5, it is characterized in that, this microbe in solid state culture apparatus also comprises that second enters pipe (123), and this second one end that enters pipe (123) is communicated with the upper strata of described casing (110), and the other end is communicated with described supervisor (150).
13. according to the microbe in solid state culture systems described in any one in claim 1 to 12, it is characterized in that, one end of described vent pipe (130) is fixed on the sidewall of described casing (110), the other end extends to the outside of described casing (110), on the sidewall of described vent pipe (130), be provided with a plurality of through holes, on the described the other end, be provided with venting port.
14. microbe in solid state culture systems according to claim 13, is characterized in that, the outside of described vent pipe (130) is coated with screen cloth (131).
15. microbe in solid state culture systems according to claim 13, it is characterized in that, this microbe in solid state culture apparatus (100) comprises discharges extension tube (132), this discharges extension tube (132) in the outside of described casing (110), and be communicated with described vent pipe (130), in described discharge extension tube (132), be provided with the second wet bulb thermometer (133).
16. microbe in solid state culture systems according to claim 13, it is characterized in that, the above-mentioned under meter (134) that is provided with of described discharge extension tube (132), this under meter (134) is electrically connected to described first flow control valve (173) and described the first controller.
17. according to the microbe in solid state culture systems described in any one in claim 1 to 12, it is characterized in that, this microbe in solid state culture systems also comprises power set (500), this power set comprises the rotation axis (510) being fixedly connected with the sidewall of described casing (110), and this rotation axis (510) can rotate around self axis.
18. microbe in solid state culture systems according to claim 17, is characterized in that, described power set comprises pulse encoder (520) and the second controller being electrically connected to this pulse encoder (520).
19. microbe in solid state culture systems according to claim 17, is characterized in that, this microbe in solid state culture systems also comprises temperature sensor (141), and this temperature sensor (141) is arranged on the bottom of described casing (110).
20. microbe in solid state culture systems according to claim 19, it is characterized in that, this microbe in solid state culture systems also comprises the brush (142) being arranged on described rotation axis and the 3rd controller being electrically connected to this brush, described rotation axis (510) can rotate relative to described brush (142), described temperature sensor (141) is electrically connected to described brush (142), and described the 3rd controller is electrically connected to described heating unit and described temperature sensor (141).
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| CN106723225A (en) * | 2016-12-29 | 2017-05-31 | 尹氏（天津）生物科技有限公司 | A kind of straw feed fermentation tank |
| CN112391270A (en) * | 2020-10-13 | 2021-02-23 | 嘉兴学院 | Rotary solid-state fermentation device with water vapor circulation and use method thereof |
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| CN103695294B (en) | 2015-08-19 |
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