CN106381267A - Simple efficient solid fermentation device - Google Patents
Simple efficient solid fermentation device Download PDFInfo
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- CN106381267A CN106381267A CN201610789997.2A CN201610789997A CN106381267A CN 106381267 A CN106381267 A CN 106381267A CN 201610789997 A CN201610789997 A CN 201610789997A CN 106381267 A CN106381267 A CN 106381267A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses a simple efficient solid fermentation device including a discharge port, a breathable material-supporting turnover plate, compressed air control valves, cloth material scraping paddles, a one-way exhaust valve, a motor, a top seat, a feed inlet, an outer cylinder body, an inner cylinder body, a stirring shaft, a first temperature measurement port, a second temperature measurement port, a thermal control device, a humidity instrument, an ultrasonic steam atomizing device and a disinfection device, wherein the motor is arranged at the upper side of the fermentation device through the top seat; the feed inlet is arranged at one side of the upper part of the fermentation device; the first temperature measurement port and the second temperature measurement port are respectively arranged at a middle part and a lower part of the fermentation device; the electric thermal control device is arranged below the first temperature measurement port; the humidity instrument is arranged at the lower part of the fermentation device; the ultrasonic steam atomizing device is arranged below the humidity instrument; the disinfection device is arranged at one side, opposite to the ultrasonic steam atomizing device, of the lower part of the fermentation device; porous regulating ventilation of compressed pure air is achieved through a plurality of compressed air control valves; the discharge port is arranged at the bottom of the fermentation device.
Description
Technical field
The invention belongs to technical field of biological fermentation, it is related to a kind of simple and efficient solid fermentation apparatus.
Background technology
Fermentation industry is that the new technology such as traditional zymotic technology and modern DNA restructuring, cell fusion combines and grows up
Modern biotechnology, and pass through modern chemistry engineering technology, produce utility or one kind of being directly used in industrialized production
Big industrial system, fermentation is divided into according to culture medium state difference：Solid fermentation and liquid fermentation.Fermentation technique is in medicine, food
The application of the aspects such as industry, environmental science, the chemical industry energy, agricultural gets more and more, and the technology application of Zymolysis Equipment is also more next therewith
More extensive.Zymolysis Equipment refers to that microbe produces the equipment of purpose product, and it must possess suitable growth of microorganism and shape
Becoming the various conditions of product, thus promoting bacteria metabolism, being allowed to obtain larger yield.
Solid fermentation method is by the use of the agronomic crop fibre crude granule such as wheat bran, rice bran, rice husk as carbon source and the energy, has
The process producing is bred in the solid state substrate top fermentation not having free water of uniform temperature and humidity.Compared with liquid state fermentation, solid-state
Fermentation has the advantage that：Wastewater treatment is not needed, the pollution to environment is little after reaction；Do not need dissolved oxygen energy resource consumption low,
Equipment is simple, small investment；Raw material sources are wide, are mostly natural substrates or waste residue, cheap；Fermentation time is short；Sweat
Gravity-flow ventilation is only needed to, energy consumption is low on the premise of controlling temperature and humidity.But solid fermentation equally there is also and sends out
Ferment occupation area of equipment is big, high labor intensive, and mass-and heat-transfer is difficult, and fermentation parameter is difficult the problems such as control, so in order to guarantee
The advantage playing solid fermentation to greatest extent, the shortcoming overcoming presence, good Zymolysis Equipment is particularly critical, how to develop one kind
Convenient use, has the solid fermentation apparatus that can improve fermentation efficiency to be exactly present invention problem to be studied.
Content of the invention
It is an object of the invention to provide a kind of simple and efficient solid fermentation apparatus, use for biofermentation.
For achieving the above object, a kind of simple and efficient solid fermentation apparatus, including discharging opening, ventilative retainer turnover panel, compression
Air shut-off valve, cloth strike off slurry, one-way exhaust valve, motor, footstock, charging aperture, outer cylinder body, inner barrel, shaft, temperature-measuring port
First, temperature-measuring port two, thermal controls apparatus, hygronom, ultrasonic steam atomising device, chlorination equipment, described motor is arranged on by footstock
On the upside of installation for fermenting, described charging aperture is arranged on installation for fermenting top side, and described temperature-measuring port one and temperature-measuring port two are respectively provided with
In middle part and the bottom of Zymolysis Equipment, described electronic thermal controls apparatus are arranged on below temperature-measuring port one, and described hygronom is arranged on to be sent out
Ferment equipment bottom, described ultrasonic steam atomising device is arranged on below hygronom, and described chlorination equipment is arranged under Zymolysis Equipment
The portion side relative with ultrasonic steam atomising device, described compression pure air is realized by multiple compressed air control valves
Porous adjusts ventilation, and described discharging opening is arranged on the bottom of Zymolysis Equipment.
Preferably, described ultrasonic steam atomising device is communicated with the interlayer of outer cylinder body and inner barrel, and described inner barrel is equal
Even porous breathable structure, can make atomizing steam enter in bucket, thus uniformly effectively adjusting fermentation humidity.
Preferably, on inner barrel outer wall at described compressed air inlet, one layer of aperture of covering is 0.05 μm of filter membrane, can
The miscellaneous bacteria of effectively catching in the air, reduces the probability of living contaminants.
Preferably, described thermal controls apparatus to realize the control of temperature, institute by way of electronic and solar energy mixing are adjusted
State thermal controls apparatus and be internally provided with temperature bound sensor, to realize the required suitable temperature ranges that ferment.
Preferably, described chlorination equipment Interior Disinfection liquid, to realize cylinder sterilization, sterilization effect by way of electric heating evaporation
Really good, overcome sterilization dead angle residue problem.
Preferably, described thimerosal is citric acid and Chinese medicine extract with 1:The thimerosal that 4 mass ratio mixes, institute
State the main component of Chinese medicine extract and extracting method is：Take Chinese medicine frutus cnidii 50-85 part, blue gum 42-78 part, gingko 37-
72 parts, smilax 30-63 part, honeysuckle 26-56 part, Radix Glycyrrhizae 20-48 part, be dried co-grinding after process, with 10-12 times
The mass concentration of amount is 70% ethanol solution refluxing extraction 3 times, and each extraction time is 30min, adopts while alcohol extract
Use microwave irradiation assisted extraction, described microwave power is 280MPa, each microwave radiation technology irradiation time is 5min, uses milipore filter mistake
Obtain filtrate after filter, described filtrate is condensed into the solution that water content is 35%, after sterilization treatment, be described Chinese medicine extract, will
Citric acid adds deionized water and dilutes in one times of addition mixer, then is added thereto Chinese medicine extract in described ratio, with 850r/
The rotating speed stirring 15min of min, as described thimerosal, no chemical residues pollute, and Long-Time Service does not interfere with fermentation condition.
Preferably, in described Fig. 1, bed of cloth thickness is 200mm, and the reaction of this thickness bottom fermentation is uniformly.
Preferably, described ventilative retainer turnover panel has 7, in FIG label 1. -7..
It is an advantage of the current invention that：A kind of present invention simple and efficient solid fermentation apparatus, structure is simple, reasonable in design, work(
Can complete, the optimization design of achievable solid fermentation, raising fermented quality and fermentation efficiency.The present invention arranges attemperating unit, can
To adjust fermentation temperature in suitable range intervals, hygronom and ultrasonic steam atomising device can ensure suitable wet of sweat
Degree, self-configuring chlorination equipment, pasteurised completely can be carried out to device, thimerosal adopts natural traditional Chinese medicine extract proportioning, decreasing
Learn the impact to fermentation condition for the residual, at compressed air inlet, adopt the harmful substance of membrane filtration in the air, it is to avoid to sending out
The impact of ferment condition.
Brief description
Fig. 1 is the positive structure schematic of simple and efficient solid fermentation apparatus；
Wherein, 1- discharging opening；2- breathes freely retainer turnover panel；3- compressed air control valve；4- cloth strikes off slurry；5- is unidirectional
Air bleeding valve；6- motor；7- footstock；8- charging aperture；9- outer cylinder body；10- inner barrel；11- shaft；12- temperature-measuring port one；13- heat
Control device；14- hygronom；15- ultrasonic steam atomising device；16- chlorination equipment；17- temperature-measuring port two.
Specific embodiment
Hereinafter, in conjunction with accompanying drawing 1 and embodiment, the specific embodiment of the present invention is described in detail：
As Fig. 1 illustrates a kind of simple and efficient solid fermentation apparatus of the present invention, including discharging opening 1, ventilative retainer turnover panel 2,
Compressed air control valve 3, cloth strike off slurry 4, one-way exhaust valve 5, motor 6, footstock 7, charging aperture 8, outer cylinder body 9, inner barrel 10,
Shaft 11, temperature-measuring port 1, temperature-measuring port 2 17, thermal controls apparatus 13, hygronom 14, ultrasonic steam atomising device 15, sterilization dress
Put 16, described motor 6 is arranged on the upside of installation for fermenting by footstock 7, described charging aperture 8 is arranged on installation for fermenting top side,
Described temperature-measuring port 1 and temperature-measuring port 2 17 are separately positioned on middle part and the bottom of Zymolysis Equipment, and described electronic thermal controls apparatus 13 set
Put below temperature-measuring port 1, described hygronom 14 is arranged on Zymolysis Equipment bottom, described ultrasonic steam atomising device 15 is arranged
Below hygronom 14, described chlorination equipment 16 is arranged on Zymolysis Equipment bottom one relative with ultrasonic steam atomising device 15
Side, described compression pure air is realized porous by multiple compressed air control valves 3 and is adjusted ventilation, and described discharging opening 1 sets
Put the bottom in Zymolysis Equipment；Described ultrasonic steam atomising device 15 is communicated with the interlayer of outer cylinder body 9 and inner barrel 10, described interior
Cylinder 10 is even porous ventilating structure, and atomizing steam can be made to enter in bucket, thus uniformly effectively adjusting fermentation humidity；Described
The filter membrane that one layer of aperture is 0.05 μm is covered on inner barrel 10 outer wall at compressed air inlet, can effectively catching in the air miscellaneous
Bacterium, reduces the probability of living contaminants；Described thermal controls apparatus 13 to realize temperature by way of electronic and solar energy mixing are adjusted
The control of degree, described thermal controls apparatus 13 are internally provided with temperature bound sensor, to realize the required suitable temperature model that ferments
Enclose；Described chlorination equipment 16 Interior Disinfection liquid, to realize cylinder sterilization by way of electric heating evaporation, and Disinfection Effect is good, overcomes
Sterilization dead angle residue problem；Described thimerosal is citric acid and Chinese medicine extract with 1:The sterilization that 4 mass ratio mixes
Liquid, the main component of described Chinese medicine extract and extracting method are：Take Chinese medicine frutus cnidii 50-85 part, blue gum 42-78 part, white
Fruit 37-72 part, smilax 30-63 part, honeysuckle 26-56 part, Radix Glycyrrhizae 20-48 part, are dried co-grinding after process, use
The mass concentration of 10-12 times amount is 70% ethanol solution refluxing extraction 3 times, and each extraction time is 30min, alcohol extract
Adopt microwave irradiation assisted extraction, described microwave power is 280MPa, each microwave radiation technology irradiation time is 5min, with surpassing simultaneously
Obtain filtrate after membrane filtration, described filtrate is condensed into the solution that water content is 35%, be described Chinese medicine after sterilization treatment and carry
Take liquid, citric acid plus deionized water diluted in one times of addition mixer, then in described ratio, Chinese medicine extract is added thereto,
15min, as described thimerosal are stirred with the rotating speed of 850r/min, no chemical residues pollute, Long-Time Service does not interfere with fermentation
Condition, no chemical residues pollute, and Long-Time Service does not interfere with fermentation condition；In described Fig. 1, bed of cloth thickness is 200mm, and this is thick
Degree bottom fermentation reaction is uniformly；Described ventilative retainer turnover panel 2 has 7, in FIG label 1. -7..
The present invention is not limited to above-mentioned embodiment, in the case of the flesh and blood without departing substantially from the present invention, this area skill
Art personnel it is conceivable that any deformation, improvement, replace each fall within the scope of the present invention.
Claims (8)
1. a kind of simple and efficient solid fermentation apparatus it is characterised in that include discharging opening (1), ventilative retainer turnover panel (2),
Compressed air control valve (3), cloth strike off slurry (4), one-way exhaust valve (5), motor (6), footstock (7), charging aperture (8), outer tube
Body (9), inner barrel (10), shaft (11), temperature-measuring port one (12), temperature-measuring port two (17), thermal controls apparatus (13), hygronom
(14), ultrasonic steam atomising device (15), chlorination equipment (16), described motor (6) is arranged on installation for fermenting by footstock (7)
Side, described charging aperture (8) is arranged on installation for fermenting top side, and described temperature-measuring port one (12) and temperature-measuring port two (17) are respectively provided with
In middle part and the bottom of Zymolysis Equipment, described electronic thermal controls apparatus (13) are arranged on below temperature-measuring port one (12), described hygronom
(14) it is arranged on Zymolysis Equipment bottom, described ultrasonic steam atomising device (15) is arranged on below hygronom (14), described sterilization
Device (16) is arranged on the Zymolysis Equipment bottom side relative with ultrasonic steam atomising device (15), the pure sky of described compression
Gas is realized porous by multiple compressed air control valves (3) and is adjusted ventilation, and described discharging opening (1) is arranged on the bottom of Zymolysis Equipment
Portion.
2. as claimed in claim 1 a kind of simple and efficient solid fermentation apparatus it is characterised in that described ultrasonic steam atomization dress
The interlayer putting (15) with outer cylinder body (9) and inner barrel (10) communicates, and described inner barrel (10) is even porous ventilating structure, can make
Atomizing steam enters in bucket.
3. as claimed in claim 1 a kind of simple and efficient solid fermentation apparatus it is characterised in that at described compressed air inlet
On inner barrel (10) outer wall, one layer of aperture of covering is 0.05 μm of filter membrane.
4. as claimed in claim 1 a kind of simple and efficient solid fermentation apparatus it is characterised in that described thermal controls apparatus (13) in
Portion is provided with temperature bound sensor.
5. a kind of simple and efficient solid fermentation apparatus described in claim 1 are it is characterised in that described chlorination equipment (16) is built-in
Thimerosal.
6. as claimed in claim 1 a kind of simple and efficient solid fermentation apparatus it is characterised in that described thimerosal be citric acid
With Chinese medicine extract with 1:The thimerosal that 4 mass ratio mixes, the main component of described Chinese medicine extract is：Frutus cnidii
50-85 part, blue gum 42-78 part, gingko 37-72 part, smilax 30-63 part, honeysuckle 26-56 part, Radix Glycyrrhizae 20-48 part.
7. as claimed in claim 1 a kind of simple and efficient solid fermentation apparatus it is characterised in that cloth thickness in described Fig. 1
Spend for 200mm.
8. as claimed in claim 1 a kind of simple and efficient solid fermentation apparatus it is characterised in that described ventilative retainer turnover panel
(2) there are 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789997.2A CN106381267B (en) | 2016-08-31 | 2016-08-31 | A kind of simple and efficient solid fermentation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789997.2A CN106381267B (en) | 2016-08-31 | 2016-08-31 | A kind of simple and efficient solid fermentation apparatus |
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| Publication Number | Publication Date |
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| CN106381267A true CN106381267A (en) | 2017-02-08 |
| CN106381267B CN106381267B (en) | 2018-01-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610789997.2A Expired - Fee Related CN106381267B (en) | 2016-08-31 | 2016-08-31 | A kind of simple and efficient solid fermentation apparatus |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102895A (en) * | 2017-12-13 | 2018-06-01 | 广西南宁秀珀生物科技有限公司 | Multiple field solid material installation for fermenting |
| CN108440043A (en) * | 2018-04-19 | 2018-08-24 | 姚永强 | A kind of bucket-tipping device |
| CN108456012A (en) * | 2018-04-19 | 2018-08-28 | 姚永强 | A kind of animal waste dystopy fermentation machine |
| CN113801782A (en) * | 2021-09-23 | 2021-12-17 | 郑州智拓生物科技有限公司 | System and method for realizing rapid culture of microbial agent |
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| CN2567225Y (en) * | 2002-08-09 | 2003-08-20 | 中国科学院过程工程研究所 | Device for maitaining high water activity in solid state fermentation process |
| CN201420071Y (en) * | 2009-05-08 | 2010-03-10 | 安徽丰原发酵技术工程研究有限公司 | Solid state fermentation device |
| CN102358883A (en) * | 2011-10-31 | 2012-02-22 | 江苏海华生物科技有限公司 | Rotary shaft type solid state fermentation tank |
| CN102732417A (en) * | 2012-06-15 | 2012-10-17 | 卢行娥 | Emulsifying-type aerobic fermentation tank |
| CN204569936U (en) * | 2015-04-24 | 2015-08-19 | 江南大学 | A kind of small-sized fermentation tank group system |
-
2016
- 2016-08-31 CN CN201610789997.2A patent/CN106381267B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2567225Y (en) * | 2002-08-09 | 2003-08-20 | 中国科学院过程工程研究所 | Device for maitaining high water activity in solid state fermentation process |
| CN201420071Y (en) * | 2009-05-08 | 2010-03-10 | 安徽丰原发酵技术工程研究有限公司 | Solid state fermentation device |
| CN102358883A (en) * | 2011-10-31 | 2012-02-22 | 江苏海华生物科技有限公司 | Rotary shaft type solid state fermentation tank |
| CN102732417A (en) * | 2012-06-15 | 2012-10-17 | 卢行娥 | Emulsifying-type aerobic fermentation tank |
| CN204569936U (en) * | 2015-04-24 | 2015-08-19 | 江南大学 | A kind of small-sized fermentation tank group system |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102895A (en) * | 2017-12-13 | 2018-06-01 | 广西南宁秀珀生物科技有限公司 | Multiple field solid material installation for fermenting |
| CN108102895B (en) * | 2017-12-13 | 2021-06-04 | 广西南宁秀珀生物科技有限公司 | Multilayer solid material fermentation device |
| CN108440043A (en) * | 2018-04-19 | 2018-08-24 | 姚永强 | A kind of bucket-tipping device |
| CN108456012A (en) * | 2018-04-19 | 2018-08-28 | 姚永强 | A kind of animal waste dystopy fermentation machine |
| CN108440043B (en) * | 2018-04-19 | 2023-07-11 | 姚永强 | Tipping bucket device |
| CN113801782A (en) * | 2021-09-23 | 2021-12-17 | 郑州智拓生物科技有限公司 | System and method for realizing rapid culture of microbial agent |
| CN113801782B (en) * | 2021-09-23 | 2023-09-01 | 郑州智拓生物科技有限公司 | System and method for realizing rapid culture of microbial agent |
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| CN106381267B (en) | 2018-01-09 |
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Granted publication date: 20180109 Termination date: 20180831 |